An investigation into the neuroprotective properties of curcumin
- Authors: Daniel, Sheril
- Date: 2003
- Subjects: Turmeric -- Therapeutic use , Nervous system -- Degeneration -- Prevention
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3753 , http://hdl.handle.net/10962/d1003231 , Turmeric -- Therapeutic use , Nervous system -- Degeneration -- Prevention
- Description: An increasing number of studies show that nutritional antioxidants such as vitamin E and polyphenols are capable of blocking neuronal death in vitro and may have therapeutic properties in animal models of neurodegenerative diseases including Alzheimer’s and Parkinson’s diseases. In the present study, the neuroprotective ability of one such polyphenolic antioxidant, curcumin, was investigated. Curcumin is the yellow curry spice derived from turmeric, and is widely used as a dietary component and herbal medicine in India. Most neurological disorders are postulated to have an oxidative or excitototoxic basis. Thus the effects of curcumin on oxidative stress in the rat brain were investigated. Curcumin, administered to the rat in vivo and in vitro, was able to exert protective effects on oxidative damage in the brain, induced by cyanide, a mitochondrial inhibitor. Curcumin also offered protection against quinolinic acid induced lipid peroxidation, and this protection was extended to lipid peroxidation induced by metals such as lead and cadmium in the rat brain. Experiments conducted on the pineal gland revealed an increased production of the neuroprotective hormone melatonin in presence of curcumin in vivo. The hippocampus is functionally related to vital behaviour and intellectual activities and is known to be a primary target for neuronal degeneration in the brains of patients with Alzheimer’s disease. Histological studies were undertaken to assess the effects of curcumin on lead induced toxicity on the rat hippocampus, the results of which show that curcumin affords significant protection to the hippocampus of the lead treated rats. This study also sought to elucidate possible mechanisms by which curcumin exerts its neuroprotective capabilities. Curcumin was found to inhibit the action of cyanide on the mitochondrial electron transport chain, one of the most common sources of free radicals. Electrochemical, UV/VIS and Infrared spectroscopy were used to characterise interactions between curcumin and the metals lead, cadmium, iron (II) and iron (III). Curcumin was shown to directly chelate these metals with the formation and isolation of two new curcumin complexes with lead, and one complex each with cadmium and iron (III). These results suggest chelation of toxic metals as a mechanism of neuroprotection afforded by curcumin. The need for neuroprotective agents is urgent considering the rapid rise in the elderly population and the proportionate increase in neurological disorders. The findings of this study indicate that curcumin, a well-established dietary antioxidant, is capable of playing a bigger role in neuroprotection, which needs to be further explored and exploited.
- Full Text:
- Date Issued: 2003
- Authors: Daniel, Sheril
- Date: 2003
- Subjects: Turmeric -- Therapeutic use , Nervous system -- Degeneration -- Prevention
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3753 , http://hdl.handle.net/10962/d1003231 , Turmeric -- Therapeutic use , Nervous system -- Degeneration -- Prevention
- Description: An increasing number of studies show that nutritional antioxidants such as vitamin E and polyphenols are capable of blocking neuronal death in vitro and may have therapeutic properties in animal models of neurodegenerative diseases including Alzheimer’s and Parkinson’s diseases. In the present study, the neuroprotective ability of one such polyphenolic antioxidant, curcumin, was investigated. Curcumin is the yellow curry spice derived from turmeric, and is widely used as a dietary component and herbal medicine in India. Most neurological disorders are postulated to have an oxidative or excitototoxic basis. Thus the effects of curcumin on oxidative stress in the rat brain were investigated. Curcumin, administered to the rat in vivo and in vitro, was able to exert protective effects on oxidative damage in the brain, induced by cyanide, a mitochondrial inhibitor. Curcumin also offered protection against quinolinic acid induced lipid peroxidation, and this protection was extended to lipid peroxidation induced by metals such as lead and cadmium in the rat brain. Experiments conducted on the pineal gland revealed an increased production of the neuroprotective hormone melatonin in presence of curcumin in vivo. The hippocampus is functionally related to vital behaviour and intellectual activities and is known to be a primary target for neuronal degeneration in the brains of patients with Alzheimer’s disease. Histological studies were undertaken to assess the effects of curcumin on lead induced toxicity on the rat hippocampus, the results of which show that curcumin affords significant protection to the hippocampus of the lead treated rats. This study also sought to elucidate possible mechanisms by which curcumin exerts its neuroprotective capabilities. Curcumin was found to inhibit the action of cyanide on the mitochondrial electron transport chain, one of the most common sources of free radicals. Electrochemical, UV/VIS and Infrared spectroscopy were used to characterise interactions between curcumin and the metals lead, cadmium, iron (II) and iron (III). Curcumin was shown to directly chelate these metals with the formation and isolation of two new curcumin complexes with lead, and one complex each with cadmium and iron (III). These results suggest chelation of toxic metals as a mechanism of neuroprotection afforded by curcumin. The need for neuroprotective agents is urgent considering the rapid rise in the elderly population and the proportionate increase in neurological disorders. The findings of this study indicate that curcumin, a well-established dietary antioxidant, is capable of playing a bigger role in neuroprotection, which needs to be further explored and exploited.
- Full Text:
- Date Issued: 2003
An investigation into the occurrence of major abscess causing bacteria at the law meat exporting abattoir in the Northern Cape province of South Africa
- Authors: Delport, Riëtte
- Date: 2003
- Subjects: Abscess , Meat -- Microbiology , Slaughtering and slaughter-houses -- South Africa -- Northern Cape
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10971 , http://hdl.handle.net/10948/249 , Abscess , Meat -- Microbiology , Slaughtering and slaughter-houses -- South Africa -- Northern Cape
- Description: The main objectives of this study can be divided into four categories: a) The determination of the presence of abscesses in carcasses. b) The determination whether the abscesses are area bound. c) The determination of the causative bacteria which is responsible for abscess formation. d) The determination of the economic impact due to carcass rejections and trimmings. The research area consists of the biggest part of the Northern Cape province, which can further be divided into three main production areas, namely Groblershoop, Gordonia and Hantam. The Groblershoop area includes Groblershoop and the surrounding districts. The Gordonia area includes Upington and the Kalahari. The Hantam area includes Calvinia, Springbok and the other districts bordering the areas. All the carcasses were slaughtered at an export abattoir, namely LAW, which is situated in Groblershoop. The Upington veterinary laboratory bacteriologically cultured the abscess material and the results were processed and noted. The following results were prominent: a) It was determined that abscesses did occur in carcasses. b) It was determined that in the Hantam main production area the prevalence of abscesses is the highest, although the slaughter numbers are the lowest. c) It was determined that the bacterium that was responsible for most of the abscesses was Escherichia coli. d) It was determined that the total occurrence of bacteria did not have a significant economic impact, because there were not many carcass rejections due to abscesses. The economic impact, however, of the value of the total rejections, might become a problem.
- Full Text:
- Date Issued: 2003
- Authors: Delport, Riëtte
- Date: 2003
- Subjects: Abscess , Meat -- Microbiology , Slaughtering and slaughter-houses -- South Africa -- Northern Cape
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10971 , http://hdl.handle.net/10948/249 , Abscess , Meat -- Microbiology , Slaughtering and slaughter-houses -- South Africa -- Northern Cape
- Description: The main objectives of this study can be divided into four categories: a) The determination of the presence of abscesses in carcasses. b) The determination whether the abscesses are area bound. c) The determination of the causative bacteria which is responsible for abscess formation. d) The determination of the economic impact due to carcass rejections and trimmings. The research area consists of the biggest part of the Northern Cape province, which can further be divided into three main production areas, namely Groblershoop, Gordonia and Hantam. The Groblershoop area includes Groblershoop and the surrounding districts. The Gordonia area includes Upington and the Kalahari. The Hantam area includes Calvinia, Springbok and the other districts bordering the areas. All the carcasses were slaughtered at an export abattoir, namely LAW, which is situated in Groblershoop. The Upington veterinary laboratory bacteriologically cultured the abscess material and the results were processed and noted. The following results were prominent: a) It was determined that abscesses did occur in carcasses. b) It was determined that in the Hantam main production area the prevalence of abscesses is the highest, although the slaughter numbers are the lowest. c) It was determined that the bacterium that was responsible for most of the abscesses was Escherichia coli. d) It was determined that the total occurrence of bacteria did not have a significant economic impact, because there were not many carcass rejections due to abscesses. The economic impact, however, of the value of the total rejections, might become a problem.
- Full Text:
- Date Issued: 2003
An investigation into the physico-chemical and neuroprotective properties of melatonin and 6-hydroxymelatonin
- Authors: Maharaj, Deepa Sukhdev
- Date: 2003
- Subjects: Melatonin Nervous system -- Degeneration -- Treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3768 , http://hdl.handle.net/10962/d1003246
- Description: Until the beginning of this decade the antioxidant, melatonin, had been considered as little more than a tranquilizing hormone, responsible for regulating certain circadian and circannual rhythms. However, it is the discovery of melatonin as a free radical scavenger that has generated the most interest in recent years. The reduction of melatonin with age has been associated with neurodegenerative diseases such as Alzheimer’s disease (AD)and therefore, melatonin has been implicated to have an important clinical role in neuroprotection. Thus, for several years melatonin has attracted increasing attention from the general press with many advertisements touting this indoleamine to act as an aphrodisiac, rejuvenator, protector against diseases and a general wonder drug. However, melatonin formulations appear with no labelling for the correct storage conditions, dosage and side effects, as well as no control for purity and self-medicating with an unregulated product. In addition, there is much controversy surrounding the antioxidative properties of the indolemaine, 6-hydroxymelatonin (6-OHM). Therefore, the first part of this study aims to elucidate the physico-chemical and various stability characteristics of the pineal antioxidant, melatonin, while the second part is devoted to investigating the neuroprotective properties of the primary hepatic metabolite of melatonin, 6-OHM. The physical properties of melatonin were determined using various chemical techniques. This information served to both characterize and confirm the identity of melatonin raw material used in this study. In addition, this information serves to be essential as the physical properties of melatonin have not been reported in detail in literature, to date. Thereafter, using a validated high performance liquid chromatography (HPLC) method, the various physico-chemical and stability characteristics of melatonin were determined. Melatonin was shown to be extremely lipophilic, while the hygroscopic study indicates that melatonin raw material is extremely hygroscopic at temperatures above 40°C, whereas melatonin tablets are hygroscopic when left out of the original container. This study highlights the need for consumers to be aware of the proper storage of melatonin tablets to improve the stability and ensure long term integrity of the compound. Since, melatonin is most often administered orally, thus exposing it to a large variations in pH, within the gastrointestinal tract, it was decided to investigate the stability of melatonin over a range of pH’s and temperatures. The findings imply that melatonin is relatively stable at body temperature when ingested orally and that orally administered slow release preparations of melatonin should be relatively stable and therefore exhibit favourable bioavailability. However melatonin was shown to be unstable in solution. This provides important information and a challenge to the formulators of this drug substance in a liquid dosage form. An assessment of the photostability of melatonin dosage forms using International Committee on Harmonization (ICH) conditions revealed melatonin to be light sensitive and thus indicates a need for careful consideration of the packaging of these drug products. In addition a detailed assessment of the photochemistry and photoproducts formed during the UV photodegradation of melatonin is reported. Melatonin is shown to rapidly degrade in the presence of UV light, with the presence of oxygen accelerating the photodegradation. N1-acetyl-N2-formyl-5-methoxykynurenamine(AFMK) and 6-OHM were identified as the major photoproducts formed and these agents have been shown previously to retain antioxidant activity. One of the concerns of using melatonin in sunscreens is its photostability. However, it is reported in this study that the degraded solution of melatonin still possesses equipotent free radical scavenging ability as melatonin, despite the absence of melatonin in solution. In addition, melatonin is shown to reduce UV-induced oxidative stress in rat skin homogenate. Thus, these results make melatonin a likely candidate for inclusion in sunscreen preparations. Neuronal damage due to oxidative stress has been implicated in several neurodegenerative disorders. 6-OHM is not only formed as the major hepatic metabolite of melatonin, but also when melatonin reacts with toxic radicals as well as UV light. Thus the second part of the study aims to elucidate and further characterize the mechanism behind 6-OHM’s neuroprotection. The results show 6-OHM to be a more potent singlet oxygen and superoxide anion scavenger than melatonin. In addition, the results show 6-OHM to offer protection against, oxidative stress and lipid peroxidation induced by several neurotoxins in the rat brain and hippocampus. The hippocampus is an important region of the brain responsible for the formation of memory and any agent that induces stress in this area has detrimental effects and could lead to various types of dementia. Such agents include quinolinic acid (QA) and iron (II). Histological studies undertaken reveal that 6-OHM is able to protect hippocampal neurons against QA and iron (II) induced necrotic cell death. Immunohistochemical investigations showed that QA moderately induces apoptotic cell death in the hippocampus which is inhibited by both melatonin and 6-OHM. The study sought to elucidate possible mechanisms by which 6-OHM exerts its neuroprotective capabilities and the results show 6-OHM to inhibit the action of cyanide on the mitochondrial electron transport chain (ETC), one of the most common sources of free radicals. In addition, 6-OHM treatment alone, increased ETC activity above basal control levels and the results show 6-OHM to increase complex I activity in the mitochondrial ETC. Electrochemical, ultraviolet/visible spectroscopy (UV/Vis) and HPLC assessment show that an interaction exists between 6-OHM and iron (III) and 6-OHM is able to reduce iron (III) to a more biologically usable form viz. iron (II) which can be incorporated into important biomolecules such as heme. One dire consequence of this interaction is the ready provision of iron (II) to drive the Fenton reaction. However the biological and histological assessments show 6-OHM to prevent iron (II)-induced lipid peroxidation and necrotic cell death and thus, provide evidence of its antioxidant properties. The results also show 6-OHM to promote Hsp70 induction in the hippocampus. Heat shock proteins, especially Hsp 70 plays a role in cytoprotection by capturing denatured proteins and facilitating the refolding of these proteins once the stress has been relieved. 6-OHM treatment alone and together with QA was shown to increase the level of expression of Hsp70, both inducible and cognate forms of the protein. This suggests that 6-OHM helps to protect against cellular protein damage induced by any form of stress the cell may encounter. Melatonin treatment alone and in combination with QA was shown to prevent increases in the level of Hsp70 in the hippocampus, indicating that melatonin was able to reduce oxidative stress induced by QA such that Hsp70 expression was not required. The discovery of neuroprotective agents, such as melatonin and 6-OHM, is becoming important considering the rapid rise in the elderly population and the proportionate increase in neurological disorders. The findings of this study indicate the need for important information regarding the correct storage conditions and stability characteristics of melatonin dosage forms. In addition, the results indicate that 6-OHM has a definite role to play as an antioxidant. Thus further research may favour the use of these agents in the treatment of several neurodegenerative disorders.
- Full Text:
- Date Issued: 2003
- Authors: Maharaj, Deepa Sukhdev
- Date: 2003
- Subjects: Melatonin Nervous system -- Degeneration -- Treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3768 , http://hdl.handle.net/10962/d1003246
- Description: Until the beginning of this decade the antioxidant, melatonin, had been considered as little more than a tranquilizing hormone, responsible for regulating certain circadian and circannual rhythms. However, it is the discovery of melatonin as a free radical scavenger that has generated the most interest in recent years. The reduction of melatonin with age has been associated with neurodegenerative diseases such as Alzheimer’s disease (AD)and therefore, melatonin has been implicated to have an important clinical role in neuroprotection. Thus, for several years melatonin has attracted increasing attention from the general press with many advertisements touting this indoleamine to act as an aphrodisiac, rejuvenator, protector against diseases and a general wonder drug. However, melatonin formulations appear with no labelling for the correct storage conditions, dosage and side effects, as well as no control for purity and self-medicating with an unregulated product. In addition, there is much controversy surrounding the antioxidative properties of the indolemaine, 6-hydroxymelatonin (6-OHM). Therefore, the first part of this study aims to elucidate the physico-chemical and various stability characteristics of the pineal antioxidant, melatonin, while the second part is devoted to investigating the neuroprotective properties of the primary hepatic metabolite of melatonin, 6-OHM. The physical properties of melatonin were determined using various chemical techniques. This information served to both characterize and confirm the identity of melatonin raw material used in this study. In addition, this information serves to be essential as the physical properties of melatonin have not been reported in detail in literature, to date. Thereafter, using a validated high performance liquid chromatography (HPLC) method, the various physico-chemical and stability characteristics of melatonin were determined. Melatonin was shown to be extremely lipophilic, while the hygroscopic study indicates that melatonin raw material is extremely hygroscopic at temperatures above 40°C, whereas melatonin tablets are hygroscopic when left out of the original container. This study highlights the need for consumers to be aware of the proper storage of melatonin tablets to improve the stability and ensure long term integrity of the compound. Since, melatonin is most often administered orally, thus exposing it to a large variations in pH, within the gastrointestinal tract, it was decided to investigate the stability of melatonin over a range of pH’s and temperatures. The findings imply that melatonin is relatively stable at body temperature when ingested orally and that orally administered slow release preparations of melatonin should be relatively stable and therefore exhibit favourable bioavailability. However melatonin was shown to be unstable in solution. This provides important information and a challenge to the formulators of this drug substance in a liquid dosage form. An assessment of the photostability of melatonin dosage forms using International Committee on Harmonization (ICH) conditions revealed melatonin to be light sensitive and thus indicates a need for careful consideration of the packaging of these drug products. In addition a detailed assessment of the photochemistry and photoproducts formed during the UV photodegradation of melatonin is reported. Melatonin is shown to rapidly degrade in the presence of UV light, with the presence of oxygen accelerating the photodegradation. N1-acetyl-N2-formyl-5-methoxykynurenamine(AFMK) and 6-OHM were identified as the major photoproducts formed and these agents have been shown previously to retain antioxidant activity. One of the concerns of using melatonin in sunscreens is its photostability. However, it is reported in this study that the degraded solution of melatonin still possesses equipotent free radical scavenging ability as melatonin, despite the absence of melatonin in solution. In addition, melatonin is shown to reduce UV-induced oxidative stress in rat skin homogenate. Thus, these results make melatonin a likely candidate for inclusion in sunscreen preparations. Neuronal damage due to oxidative stress has been implicated in several neurodegenerative disorders. 6-OHM is not only formed as the major hepatic metabolite of melatonin, but also when melatonin reacts with toxic radicals as well as UV light. Thus the second part of the study aims to elucidate and further characterize the mechanism behind 6-OHM’s neuroprotection. The results show 6-OHM to be a more potent singlet oxygen and superoxide anion scavenger than melatonin. In addition, the results show 6-OHM to offer protection against, oxidative stress and lipid peroxidation induced by several neurotoxins in the rat brain and hippocampus. The hippocampus is an important region of the brain responsible for the formation of memory and any agent that induces stress in this area has detrimental effects and could lead to various types of dementia. Such agents include quinolinic acid (QA) and iron (II). Histological studies undertaken reveal that 6-OHM is able to protect hippocampal neurons against QA and iron (II) induced necrotic cell death. Immunohistochemical investigations showed that QA moderately induces apoptotic cell death in the hippocampus which is inhibited by both melatonin and 6-OHM. The study sought to elucidate possible mechanisms by which 6-OHM exerts its neuroprotective capabilities and the results show 6-OHM to inhibit the action of cyanide on the mitochondrial electron transport chain (ETC), one of the most common sources of free radicals. In addition, 6-OHM treatment alone, increased ETC activity above basal control levels and the results show 6-OHM to increase complex I activity in the mitochondrial ETC. Electrochemical, ultraviolet/visible spectroscopy (UV/Vis) and HPLC assessment show that an interaction exists between 6-OHM and iron (III) and 6-OHM is able to reduce iron (III) to a more biologically usable form viz. iron (II) which can be incorporated into important biomolecules such as heme. One dire consequence of this interaction is the ready provision of iron (II) to drive the Fenton reaction. However the biological and histological assessments show 6-OHM to prevent iron (II)-induced lipid peroxidation and necrotic cell death and thus, provide evidence of its antioxidant properties. The results also show 6-OHM to promote Hsp70 induction in the hippocampus. Heat shock proteins, especially Hsp 70 plays a role in cytoprotection by capturing denatured proteins and facilitating the refolding of these proteins once the stress has been relieved. 6-OHM treatment alone and together with QA was shown to increase the level of expression of Hsp70, both inducible and cognate forms of the protein. This suggests that 6-OHM helps to protect against cellular protein damage induced by any form of stress the cell may encounter. Melatonin treatment alone and in combination with QA was shown to prevent increases in the level of Hsp70 in the hippocampus, indicating that melatonin was able to reduce oxidative stress induced by QA such that Hsp70 expression was not required. The discovery of neuroprotective agents, such as melatonin and 6-OHM, is becoming important considering the rapid rise in the elderly population and the proportionate increase in neurological disorders. The findings of this study indicate the need for important information regarding the correct storage conditions and stability characteristics of melatonin dosage forms. In addition, the results indicate that 6-OHM has a definite role to play as an antioxidant. Thus further research may favour the use of these agents in the treatment of several neurodegenerative disorders.
- Full Text:
- Date Issued: 2003
An investigation into the relationship marketing strategies applied by Eastern Cape based travel agents
- Authors: Fisher, Gary
- Date: 2003
- Subjects: Relationship marketing -- South Africa -- Eastern Cape , Travel agents -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MTech (Marketing)
- Identifier: vital:10766 , http://hdl.handle.net/10948/251 , Relationship marketing -- South Africa -- Eastern Cape , Travel agents -- South Africa -- Eastern Cape
- Description: Relationship marketing has grown in importance. This growth affects all industries, including the tourism, hospitality and travel industry. Of particular importance is the bond of relationship marketing between suppliers (principals) and retailers (travel agents) in the distribution channel. The research was undertaken to: determine whether Eastern Cape travel agents perceive relationship marketing as important; establish, what the key elements of relationship marketing strategies are,according to literature; determine what marketing strategies are currently applied by Eastern Cape travel agents; provide guidelines to Eastern Cape travel agents to implement or improve their relationship marketing strategies. Fifty self-administered questionnaires were distributed to the entire population of travel agents in the Eastern Cape. A total of 30 travel agents responded. The empirical findings showed that: Most Eastern Cape travel agents perceived relationship marketing as important; Travel agents realized the importance of the management of such relationship marketing strategies; Most travel agents were aware of or used retention strategies to enhance relationship marketing strategies; Travel agents realized that to survive relationship marketing should be part of their future planning; All respondents believed that to successfully implement relationship marketing, all staff, from front-line to top-management, need to be committed to the programme. In conclusion, relationship marketing is growing in importance and all role players need to acknowledge and manage this trend. However, this research has shown that there is a paradigm shift as more travel agents are moving away from the traditional marketing to relationship marketing.
- Full Text:
- Date Issued: 2003
- Authors: Fisher, Gary
- Date: 2003
- Subjects: Relationship marketing -- South Africa -- Eastern Cape , Travel agents -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MTech (Marketing)
- Identifier: vital:10766 , http://hdl.handle.net/10948/251 , Relationship marketing -- South Africa -- Eastern Cape , Travel agents -- South Africa -- Eastern Cape
- Description: Relationship marketing has grown in importance. This growth affects all industries, including the tourism, hospitality and travel industry. Of particular importance is the bond of relationship marketing between suppliers (principals) and retailers (travel agents) in the distribution channel. The research was undertaken to: determine whether Eastern Cape travel agents perceive relationship marketing as important; establish, what the key elements of relationship marketing strategies are,according to literature; determine what marketing strategies are currently applied by Eastern Cape travel agents; provide guidelines to Eastern Cape travel agents to implement or improve their relationship marketing strategies. Fifty self-administered questionnaires were distributed to the entire population of travel agents in the Eastern Cape. A total of 30 travel agents responded. The empirical findings showed that: Most Eastern Cape travel agents perceived relationship marketing as important; Travel agents realized the importance of the management of such relationship marketing strategies; Most travel agents were aware of or used retention strategies to enhance relationship marketing strategies; Travel agents realized that to survive relationship marketing should be part of their future planning; All respondents believed that to successfully implement relationship marketing, all staff, from front-line to top-management, need to be committed to the programme. In conclusion, relationship marketing is growing in importance and all role players need to acknowledge and manage this trend. However, this research has shown that there is a paradigm shift as more travel agents are moving away from the traditional marketing to relationship marketing.
- Full Text:
- Date Issued: 2003
An investigation into whether a leadership and management development project changed the perceptions of the educators in a black, disadvantaged school
- Authors: Jaftha, A P
- Date: 2003
- Subjects: University of Port Elizabeth -- Institute for Development Planning and Research Delta Foundation -- Centres of Learning School management and organization -- South Africa Educational leadership -- South Africa Educational change -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1620 , http://hdl.handle.net/10962/d1003502
- Description: This study investigates whether a leadership and management development programme changed the management perceptions of the teachers of one of the schools that participated in the programme. The programme seeks to change the organisational culture of schools and develop effective management. The development philosophy of the programme is informed by literature on school effectiveness, organisation development, whole school development and systemic change. My research is conducted in the interpretive paradigm. Interpretive researchers view reality a construct of the human mind, and therefore the way people perceive the world is not necessarily the same since there can be different understandings of what is real. I employ convenience sampling to select the school and purposive sampling to identify my research participants. Whilst overall impression that the data conveys is that much of the programme does not seem to have had a lasting effect on the culture of the school, it nonetheless made an impression on the teachers’ perceptions of management. There clearly seems to be a greater appreciation of participative management processes. The reintroduction of proper prior planning played a decisive role in re-establishing sound work ethics at the school and contributes to a more structured approach to events at the school. Some sort of organisational learning has also been introduced at the school. The realisation on the part of the staff that they now have their destiny in their own hands is an especially critical development. And whilst the vision is not a compelling force in the organisation, it has nevertheless produced some strategic focus at the school. The insights and skills they acquired concerning resource management will also stand them in good stead.
- Full Text:
- Date Issued: 2003
- Authors: Jaftha, A P
- Date: 2003
- Subjects: University of Port Elizabeth -- Institute for Development Planning and Research Delta Foundation -- Centres of Learning School management and organization -- South Africa Educational leadership -- South Africa Educational change -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1620 , http://hdl.handle.net/10962/d1003502
- Description: This study investigates whether a leadership and management development programme changed the management perceptions of the teachers of one of the schools that participated in the programme. The programme seeks to change the organisational culture of schools and develop effective management. The development philosophy of the programme is informed by literature on school effectiveness, organisation development, whole school development and systemic change. My research is conducted in the interpretive paradigm. Interpretive researchers view reality a construct of the human mind, and therefore the way people perceive the world is not necessarily the same since there can be different understandings of what is real. I employ convenience sampling to select the school and purposive sampling to identify my research participants. Whilst overall impression that the data conveys is that much of the programme does not seem to have had a lasting effect on the culture of the school, it nonetheless made an impression on the teachers’ perceptions of management. There clearly seems to be a greater appreciation of participative management processes. The reintroduction of proper prior planning played a decisive role in re-establishing sound work ethics at the school and contributes to a more structured approach to events at the school. Some sort of organisational learning has also been introduced at the school. The realisation on the part of the staff that they now have their destiny in their own hands is an especially critical development. And whilst the vision is not a compelling force in the organisation, it has nevertheless produced some strategic focus at the school. The insights and skills they acquired concerning resource management will also stand them in good stead.
- Full Text:
- Date Issued: 2003
An investigation of code switching in different learning areas in a grade seven class
- Authors: Ndabeni, Bulelwa
- Date: 2003
- Subjects: Code switching (Linguistics) -- South Africa Language and education -- South Africa Education, Bilingual -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1621 , http://hdl.handle.net/10962/d1003503
- Description: The research described in this thesis is a case study of the communicative repertoires of teachers and learners of a grade seven class in a small rural primary school. The aim of the investigation was to find out if code switching is different in content-subject classes than in an English language class. The study accomplishes this by looking at code switching in different learning areas. Different research techniques such as observations, video. recording, taking of field notes and interviews are used as the means of data collection. The study does not attempt a full linguistic description of the switches made by the research subjects. For example, it does not deal with linguistic constraints on code switching, but instead it focuses on the functional aspects of code switching. The study reveals that there are various functions of code switching in the classroom. The following are the major findings noted in this study: • Code switching is a communicative resource, which enables the teachers and learners to accomplish a considerable number and range of social and educational objectives. • Code switching is used to negotiate and renegotiate meaning. • How teachers code switch in class depends on how difficult the lesson is for the learners. • The study reveals no major differences between code switching In content subjects than in English as a subject. • Although tbe subjects engage in code switching, they seem to feel guilty about their behaviour.
- Full Text:
- Date Issued: 2003
- Authors: Ndabeni, Bulelwa
- Date: 2003
- Subjects: Code switching (Linguistics) -- South Africa Language and education -- South Africa Education, Bilingual -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1621 , http://hdl.handle.net/10962/d1003503
- Description: The research described in this thesis is a case study of the communicative repertoires of teachers and learners of a grade seven class in a small rural primary school. The aim of the investigation was to find out if code switching is different in content-subject classes than in an English language class. The study accomplishes this by looking at code switching in different learning areas. Different research techniques such as observations, video. recording, taking of field notes and interviews are used as the means of data collection. The study does not attempt a full linguistic description of the switches made by the research subjects. For example, it does not deal with linguistic constraints on code switching, but instead it focuses on the functional aspects of code switching. The study reveals that there are various functions of code switching in the classroom. The following are the major findings noted in this study: • Code switching is a communicative resource, which enables the teachers and learners to accomplish a considerable number and range of social and educational objectives. • Code switching is used to negotiate and renegotiate meaning. • How teachers code switch in class depends on how difficult the lesson is for the learners. • The study reveals no major differences between code switching In content subjects than in English as a subject. • Although tbe subjects engage in code switching, they seem to feel guilty about their behaviour.
- Full Text:
- Date Issued: 2003
An investigation of female leaders' perceptions of themselves and their roles as leaders in a Catholic School
- Authors: Kauaria, Vejanda
- Date: 2003
- Subjects: Catholic schools -- Namibia -- Administration Women school administrators -- Namibia School management and organization -- Namibia Educational leadership -- Namibia Women in public life -- Namibia Sex discrimination against women -- Namibia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1626 , http://hdl.handle.net/10962/d1003508
- Description: The question of gender in leadership continues to be a contentious and poorly understood phenomenon. There seems general agreement that women do lead differently from men, and this study begins from that premise, focusing on a case in which leadership has traditionally been the domain of females. The case was chosen for its uniqueness, the assumption being that in these circumstances leadership may have developed particular characteristics. Following a qualitative approach (drawing on phenomenology), the study seeks to investigate how women leaders experience their roles as leaders. In-depth interviews made it possible for me to capture the perceptions and experiences of the three women leaders I interviewed. The study reveals that women are more inclined to use interactive styles of leadership. Women use leadership that is more participative, negotiative, cooperative, shared and collaborative. These characteristics are in line with the features of transformational leadership which differs from the more traditional transactional leadership that is more controlled and directive. The study has also shown that leadership develops from within the person of the leader as the leader is the one who spearheads the organization through vision, ideas, beliefs andassumptions. The findings of this study suggest thus that unless women are given chances to prove how they can lead, this new approach of leadership within them and that is required by modern organizations would be lost and leadership would remain relatively unchanged and undesirable. In the context of Namibia, this study should be of potential significance because of the rapid change that is taking place in the inclusion of women in leadership and management positions in education.
- Full Text:
- Date Issued: 2003
- Authors: Kauaria, Vejanda
- Date: 2003
- Subjects: Catholic schools -- Namibia -- Administration Women school administrators -- Namibia School management and organization -- Namibia Educational leadership -- Namibia Women in public life -- Namibia Sex discrimination against women -- Namibia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1626 , http://hdl.handle.net/10962/d1003508
- Description: The question of gender in leadership continues to be a contentious and poorly understood phenomenon. There seems general agreement that women do lead differently from men, and this study begins from that premise, focusing on a case in which leadership has traditionally been the domain of females. The case was chosen for its uniqueness, the assumption being that in these circumstances leadership may have developed particular characteristics. Following a qualitative approach (drawing on phenomenology), the study seeks to investigate how women leaders experience their roles as leaders. In-depth interviews made it possible for me to capture the perceptions and experiences of the three women leaders I interviewed. The study reveals that women are more inclined to use interactive styles of leadership. Women use leadership that is more participative, negotiative, cooperative, shared and collaborative. These characteristics are in line with the features of transformational leadership which differs from the more traditional transactional leadership that is more controlled and directive. The study has also shown that leadership develops from within the person of the leader as the leader is the one who spearheads the organization through vision, ideas, beliefs andassumptions. The findings of this study suggest thus that unless women are given chances to prove how they can lead, this new approach of leadership within them and that is required by modern organizations would be lost and leadership would remain relatively unchanged and undesirable. In the context of Namibia, this study should be of potential significance because of the rapid change that is taking place in the inclusion of women in leadership and management positions in education.
- Full Text:
- Date Issued: 2003
An investigation of knowledge management implementation strategies
- Authors: Sunassee, Nakkiran N
- Date: 2003
- Subjects: Knowledge management , Strategic planning , Automobile industry and trade -- South Africa -- Management
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:1141 , http://hdl.handle.net/10962/d1002770 , Knowledge management , Strategic planning , Automobile industry and trade -- South Africa -- Management
- Description: The world is experiencing an era which has been termed the “knowledge age” or the “knowledge economy”. In this new context, knowledge is the primary commodity, and knowledge flows are regarded as the most important factors in the economy. The management of knowledge flows within organisations has become a crucial activity because many of the activities of organisations and the broader economic and social life today are knowledge-driven. In recent years, this managerial activity has become known as Knowledge Management. Equally important are the associated Knowledge Management implementation strategies. Driven by a need expressed by the South African Motor Vehicle Industry for a knowledge management implementation strategy tailored to their needs, this research aimed to develop a knowledge management implementation strategy suited to the needs of this industry. Following an extensive literature survey and a study of the target industry using Duffy’s Knowledge Benchmarking Questionnaire, a model of knowledge management implementation is proposed that is suited to the needs of the South African Motor Vehicle Industry. The model consists of three main interlinked components: Knowledge Management of the Organisation, People, and Infrastructure and Processes. Furthermore, the model recommends a holistic approach to managing knowledge. The critical success factors of the model were tested by means of a survey of industry opinions that validated certain aspects of the model and motivated for changes in others. Additionally, despite the focus of the model on the target industry, it is considered sufficiently appropriate for use by other organisations.
- Full Text:
- Date Issued: 2003
- Authors: Sunassee, Nakkiran N
- Date: 2003
- Subjects: Knowledge management , Strategic planning , Automobile industry and trade -- South Africa -- Management
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:1141 , http://hdl.handle.net/10962/d1002770 , Knowledge management , Strategic planning , Automobile industry and trade -- South Africa -- Management
- Description: The world is experiencing an era which has been termed the “knowledge age” or the “knowledge economy”. In this new context, knowledge is the primary commodity, and knowledge flows are regarded as the most important factors in the economy. The management of knowledge flows within organisations has become a crucial activity because many of the activities of organisations and the broader economic and social life today are knowledge-driven. In recent years, this managerial activity has become known as Knowledge Management. Equally important are the associated Knowledge Management implementation strategies. Driven by a need expressed by the South African Motor Vehicle Industry for a knowledge management implementation strategy tailored to their needs, this research aimed to develop a knowledge management implementation strategy suited to the needs of this industry. Following an extensive literature survey and a study of the target industry using Duffy’s Knowledge Benchmarking Questionnaire, a model of knowledge management implementation is proposed that is suited to the needs of the South African Motor Vehicle Industry. The model consists of three main interlinked components: Knowledge Management of the Organisation, People, and Infrastructure and Processes. Furthermore, the model recommends a holistic approach to managing knowledge. The critical success factors of the model were tested by means of a survey of industry opinions that validated certain aspects of the model and motivated for changes in others. Additionally, despite the focus of the model on the target industry, it is considered sufficiently appropriate for use by other organisations.
- Full Text:
- Date Issued: 2003
An investigation of knowledge management practices in the Old Mutual, Buffalo City Metropole branch
- Authors: Siqoko, Kolisa
- Date: 2003
- Subjects: Knowledge management , Insurance companies -- South Africa -- East London
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10879 , http://hdl.handle.net/10948/252 , Knowledge management , Insurance companies -- South Africa -- East London
- Description: The study has sought to examine knowledge management practice in the Old Mutual, Buffalo City Metropole. The study has been conducted against the backdrop of measuring how far South African companies are in applying Knowledge Management (KM) tools and techniques to improve their competitive advantage. The study was conducted based on the assumption that insurance companies in South Africa are at the forefront in the use and application of technology. Various concepts are defined throughout the study. In reviewing literature for example, the study introduces the reader to various theories, views and approaches to KM. The most significant of these are the cultural and social systems approach on one hand and the product or process approach on the other. The study relied on the use of a variety of data collection methods such as questionnaires, interviews and participant observation for the purposes of collecting primary data. From the primary data collected, it was discovered that the largest number of responses came from marketing, sales and customer services departments. The employees have limited flexibility in terms of decision making and team work, due to the rigidity of tasks. The findings revealed that the company has a loosely structured infrastructure, where each strategic business unit (SBU) manages its own IT tools and documents. All these underlying aspects are not conducive to the promotion of and growth of KM in any company. The final chapter concludes with a model for KM implementation and the procedures to be followed to ensure that the practice adds value to the company.
- Full Text:
- Date Issued: 2003
- Authors: Siqoko, Kolisa
- Date: 2003
- Subjects: Knowledge management , Insurance companies -- South Africa -- East London
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10879 , http://hdl.handle.net/10948/252 , Knowledge management , Insurance companies -- South Africa -- East London
- Description: The study has sought to examine knowledge management practice in the Old Mutual, Buffalo City Metropole. The study has been conducted against the backdrop of measuring how far South African companies are in applying Knowledge Management (KM) tools and techniques to improve their competitive advantage. The study was conducted based on the assumption that insurance companies in South Africa are at the forefront in the use and application of technology. Various concepts are defined throughout the study. In reviewing literature for example, the study introduces the reader to various theories, views and approaches to KM. The most significant of these are the cultural and social systems approach on one hand and the product or process approach on the other. The study relied on the use of a variety of data collection methods such as questionnaires, interviews and participant observation for the purposes of collecting primary data. From the primary data collected, it was discovered that the largest number of responses came from marketing, sales and customer services departments. The employees have limited flexibility in terms of decision making and team work, due to the rigidity of tasks. The findings revealed that the company has a loosely structured infrastructure, where each strategic business unit (SBU) manages its own IT tools and documents. All these underlying aspects are not conducive to the promotion of and growth of KM in any company. The final chapter concludes with a model for KM implementation and the procedures to be followed to ensure that the practice adds value to the company.
- Full Text:
- Date Issued: 2003
An investigation of the isolation, characterisation and application of hydantoinases for the industrial production of amino acids
- Authors: Kirchmann, Shaun
- Date: 2003
- Subjects: Hydantoin Amino acids Hydrolysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3969 , http://hdl.handle.net/10962/d1004028
- Description: This thesis describes a series of investigations into the hydantoin-hydrolysing activity of bacterial strains RU-KM1 and RU-OR, which were previously isolated for their ability to hydrolyse hydantoins to amino acids. The main aim of the study was to develop biotransformations with potential application in the production of enantiomerically pure amino acids using a bioreactor based system utilising the hydantoin hydrolysing enzymes of the two isolated microorganisms. Different substituted hydantoins may be used as substrates by these enzymes for the production of a variety of amino acids. These are not only important for amino acid production, but they may be used for production of other industrially important compounds, such as semisynthetic penicillin/ampicillin, L-aspartame (sweetener), Fluvalinate (insecticide), Enalapril (ACE inhibitor). Thus, the ability of the above-mentioned strains to hydrolyse these substrates was investigated, with the view to utilizing the maximum potential of these biocatalysts. Hydantoin conversion involves a two-step hydrolysis reaction which yields, initially, an N-carbamylamino acid intermediate, and subsequently, an amino acid. The hydantoin-hydrolysing enzymes of a Pseudomonas sp. RU-KM1, and an Agrobacterium sp. RU-OR were characterised as whole cells and in a crude extract preparation, and reaction conditions for its biocatalytic application were optimised. The optimum conditions for conversion of hydantoin to glycine were found to be 1 hour at 40 °C, with conversion yields greater than 30 % achieved. The enzymes of RU-KM1 demonstrated considerable stability, retaining 80 % of their activity after storage for 2 weeks at 4 °C. The activities of the enzymes were increased by the addition of a detergent to the extraction medium, suggesting that the enzymes might be membrane-bound. The results of the determination of the metal-dependence of the hydantoinase and N-carbamoylase of RU-KM1 suggested that these enzymes required metal ions for activity, with metal ions such as Cu[superscript (2+)], Fe[superscript (2+)], and Co[superscript (2+)] resulting in no significant change in enzyme activity, however there was an activation of the enzymes when Mn[superscript (2+)] was added to the enzymes. The stereoselectivity of the enzymes was investigated, and the results suggested that the hydantoinase was D-selective, whereas the N-carbamoylase was shown to be L-selective by other researchers. The hydantoin substrate selectivity of RU-KM1 and RU-OR was investigated, and the organisms were shown to be able to hydrolyse all of the seven substrates tested. However, there was a difference in activity levels between crude extract preparations and whole cells, with crude extracts generally showing slightly lower activity than whole cells in RU-KM1, and the whole cells or RU-OR showing the lower activity than its crude extract. Some difference was also observed in the order of preference of substrates between whole cells and crude extracts. The preferred substrate for RU-KM1 whole cells was isopropylhydantoin, whereas the crude extract preparation preferentially hydrolysed p-hydroxyphenylhydantoin, achieving 57 % and 52 % conversions respectively. RU-OR whole cells preferred methylhydantoin where as the crude extract preferred isopropylhydantoin, and showed 49 % and 51 % conversions respectively. The enzymes were characterised in terms of their temperature and pH optima, inducer requirements, and product inhibition studies. The hydantoinase of RU-KM1 was shown to be inducible with low levels of hydantoin, and thermostable upto 75 °C with its optima between 60 and 70 °C. The N-carbamoylase was shown to have its optima at 50 °C. The addition of ATP (0.5 mM), DTT (1 mM) and a protease inhibitor (2 mg.mL[superscript (-1)]) all increased the hydantoinase activity of RU-KM1 crude extract, however they had very little effect on the N-carbamoylase activity. The hydantoinase enzyme from extracts of RU-KM1 was partially purified by development of cell disruption methods using mechanical and lysing enzymes, followed by precipitation and chromatographic resolution. The results obtained showed a hydantoinase enzyme of between 48 and 66 kDa. RU-KM1 was grown under fermentation conditions using different minimal media. The activity and yields under these conditions were low. Previous attempt to grow the organism in a rich medium had resulted in an increase in biomass but no hydantoinase activity. A rich medium was developed by carbon and nitrogen optimisation and yielded biomass up to 30 g.L[superscript (-1)] dry cell weight. The hydantoinase activity was restored by nitrogen starvation in stationary phase. This resulted in high biomass with increased activity. This data is currently in press. Crude extract and whole cells were immobilised on flat sheet membranes, hollow fibre membranes and in alginate beads. Low hydantoinase activity was measured in bioreactors using membranes in different configurations. A significant increase in hydantoinase activity was measured when the crude extract was immobilised in sodium alginate, as a result of stabilisation of the N-carbamoylase. Temperature and pH optima were unaffected by the immobilisation procedure, however the durability of the enzymes increased 2-fold. Different configurations of the bioreactor were investigated, as well as a hydroxyphenylhydantoin as an alternative substrate in this study. The bioreactors showed a near 95 % conversion of the hydantoin to glycine, and a 99 % conversion using HPG. In conclusion, the hydantoin-hydrolysing enzymes of RU-KM1 have been shown to be possibly membrane associated, which is a novel finding. This study has shown that the hydantoinase of RU-KM1 is D-stereoselective, with high temperature stability. A growth medium was developed for the rapid production of active biomass. A bioreactor was developed using a single and a dual biocatalyst configuration, which was capable of hydrolysing hydantoin and monosubstituted hydantoins to produce amino acids. To our knowledge this system is the first such dual biocatalyst system reported for the production of amino acids.
- Full Text:
- Date Issued: 2003
- Authors: Kirchmann, Shaun
- Date: 2003
- Subjects: Hydantoin Amino acids Hydrolysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3969 , http://hdl.handle.net/10962/d1004028
- Description: This thesis describes a series of investigations into the hydantoin-hydrolysing activity of bacterial strains RU-KM1 and RU-OR, which were previously isolated for their ability to hydrolyse hydantoins to amino acids. The main aim of the study was to develop biotransformations with potential application in the production of enantiomerically pure amino acids using a bioreactor based system utilising the hydantoin hydrolysing enzymes of the two isolated microorganisms. Different substituted hydantoins may be used as substrates by these enzymes for the production of a variety of amino acids. These are not only important for amino acid production, but they may be used for production of other industrially important compounds, such as semisynthetic penicillin/ampicillin, L-aspartame (sweetener), Fluvalinate (insecticide), Enalapril (ACE inhibitor). Thus, the ability of the above-mentioned strains to hydrolyse these substrates was investigated, with the view to utilizing the maximum potential of these biocatalysts. Hydantoin conversion involves a two-step hydrolysis reaction which yields, initially, an N-carbamylamino acid intermediate, and subsequently, an amino acid. The hydantoin-hydrolysing enzymes of a Pseudomonas sp. RU-KM1, and an Agrobacterium sp. RU-OR were characterised as whole cells and in a crude extract preparation, and reaction conditions for its biocatalytic application were optimised. The optimum conditions for conversion of hydantoin to glycine were found to be 1 hour at 40 °C, with conversion yields greater than 30 % achieved. The enzymes of RU-KM1 demonstrated considerable stability, retaining 80 % of their activity after storage for 2 weeks at 4 °C. The activities of the enzymes were increased by the addition of a detergent to the extraction medium, suggesting that the enzymes might be membrane-bound. The results of the determination of the metal-dependence of the hydantoinase and N-carbamoylase of RU-KM1 suggested that these enzymes required metal ions for activity, with metal ions such as Cu[superscript (2+)], Fe[superscript (2+)], and Co[superscript (2+)] resulting in no significant change in enzyme activity, however there was an activation of the enzymes when Mn[superscript (2+)] was added to the enzymes. The stereoselectivity of the enzymes was investigated, and the results suggested that the hydantoinase was D-selective, whereas the N-carbamoylase was shown to be L-selective by other researchers. The hydantoin substrate selectivity of RU-KM1 and RU-OR was investigated, and the organisms were shown to be able to hydrolyse all of the seven substrates tested. However, there was a difference in activity levels between crude extract preparations and whole cells, with crude extracts generally showing slightly lower activity than whole cells in RU-KM1, and the whole cells or RU-OR showing the lower activity than its crude extract. Some difference was also observed in the order of preference of substrates between whole cells and crude extracts. The preferred substrate for RU-KM1 whole cells was isopropylhydantoin, whereas the crude extract preparation preferentially hydrolysed p-hydroxyphenylhydantoin, achieving 57 % and 52 % conversions respectively. RU-OR whole cells preferred methylhydantoin where as the crude extract preferred isopropylhydantoin, and showed 49 % and 51 % conversions respectively. The enzymes were characterised in terms of their temperature and pH optima, inducer requirements, and product inhibition studies. The hydantoinase of RU-KM1 was shown to be inducible with low levels of hydantoin, and thermostable upto 75 °C with its optima between 60 and 70 °C. The N-carbamoylase was shown to have its optima at 50 °C. The addition of ATP (0.5 mM), DTT (1 mM) and a protease inhibitor (2 mg.mL[superscript (-1)]) all increased the hydantoinase activity of RU-KM1 crude extract, however they had very little effect on the N-carbamoylase activity. The hydantoinase enzyme from extracts of RU-KM1 was partially purified by development of cell disruption methods using mechanical and lysing enzymes, followed by precipitation and chromatographic resolution. The results obtained showed a hydantoinase enzyme of between 48 and 66 kDa. RU-KM1 was grown under fermentation conditions using different minimal media. The activity and yields under these conditions were low. Previous attempt to grow the organism in a rich medium had resulted in an increase in biomass but no hydantoinase activity. A rich medium was developed by carbon and nitrogen optimisation and yielded biomass up to 30 g.L[superscript (-1)] dry cell weight. The hydantoinase activity was restored by nitrogen starvation in stationary phase. This resulted in high biomass with increased activity. This data is currently in press. Crude extract and whole cells were immobilised on flat sheet membranes, hollow fibre membranes and in alginate beads. Low hydantoinase activity was measured in bioreactors using membranes in different configurations. A significant increase in hydantoinase activity was measured when the crude extract was immobilised in sodium alginate, as a result of stabilisation of the N-carbamoylase. Temperature and pH optima were unaffected by the immobilisation procedure, however the durability of the enzymes increased 2-fold. Different configurations of the bioreactor were investigated, as well as a hydroxyphenylhydantoin as an alternative substrate in this study. The bioreactors showed a near 95 % conversion of the hydantoin to glycine, and a 99 % conversion using HPG. In conclusion, the hydantoin-hydrolysing enzymes of RU-KM1 have been shown to be possibly membrane associated, which is a novel finding. This study has shown that the hydantoinase of RU-KM1 is D-stereoselective, with high temperature stability. A growth medium was developed for the rapid production of active biomass. A bioreactor was developed using a single and a dual biocatalyst configuration, which was capable of hydrolysing hydantoin and monosubstituted hydantoins to produce amino acids. To our knowledge this system is the first such dual biocatalyst system reported for the production of amino acids.
- Full Text:
- Date Issued: 2003
An investigation of the relative importance of the media in influencing the voting behaviour of Evelyn Hone College students in Lusaka during the December, 2001 presidential elections in Zambia
- Authors: Jere, Caesar
- Date: 2003
- Subjects: Voting -- Zambia Mass media -- Political aspects -- Zambia Presidents -- Zambia -- Election -- 2001
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3516 , http://hdl.handle.net/10962/d1007803
- Description: This study explores the relative importance of the media in influencing the electoral choices of a small group of Zambians, namely Evelyn Hone College students in Lusaka who participated in the December 2001 elections in Zambia. The study investigates the interplay of mediation between the lived cultural experiences of the sample of students and the impact of media messages. Students at Evelyn Hone College come from different ethnic groupings, social classes, religions and regions. They are exposed to both the state and the privately owned media, which represent a range of political viewpoints. The outcome of the December 2001 elections in Zambia gave the impression that people in urban areas in Zambia were less likely to vote on ethnic lines because they had easy access to different media, ranging from print to electronic, which provided them with diverse information about the political scene, and probably shaped their electoral choices. Conversely, their counterparts in the rural areas appeared to vote along ethnic lines, seemingly because of lack of exposure to the media. It was also assumed that most people in rural areas were not as modernized as their urban counterparts who were more exposed to enlightened sources of information such as professional groups and other elite social institutions which helped them to shape their political opinions than most rural people. The study attempts to establish to what extent the media impacted on the selected sample of urban students in the choice of their presidential candidates in the December 2001 elections in Zambia. The study further investigates to what extent other factors such as ethnic inclinations and other social predispositions influenced them in their choice of the candidates. The interviewees for this study were randomly drawn from a population of Evelyn Hone College students that voted in the December 2001 presidential elections in Zambia. The sample consisted of 30 randomly selected students who were purposely stratified in three focus groups of ten each. Each stratum represented the approximate ethnic equivalence of one of the presidential candidates who contested the December 2001 elections.
- Full Text:
- Date Issued: 2003
- Authors: Jere, Caesar
- Date: 2003
- Subjects: Voting -- Zambia Mass media -- Political aspects -- Zambia Presidents -- Zambia -- Election -- 2001
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3516 , http://hdl.handle.net/10962/d1007803
- Description: This study explores the relative importance of the media in influencing the electoral choices of a small group of Zambians, namely Evelyn Hone College students in Lusaka who participated in the December 2001 elections in Zambia. The study investigates the interplay of mediation between the lived cultural experiences of the sample of students and the impact of media messages. Students at Evelyn Hone College come from different ethnic groupings, social classes, religions and regions. They are exposed to both the state and the privately owned media, which represent a range of political viewpoints. The outcome of the December 2001 elections in Zambia gave the impression that people in urban areas in Zambia were less likely to vote on ethnic lines because they had easy access to different media, ranging from print to electronic, which provided them with diverse information about the political scene, and probably shaped their electoral choices. Conversely, their counterparts in the rural areas appeared to vote along ethnic lines, seemingly because of lack of exposure to the media. It was also assumed that most people in rural areas were not as modernized as their urban counterparts who were more exposed to enlightened sources of information such as professional groups and other elite social institutions which helped them to shape their political opinions than most rural people. The study attempts to establish to what extent the media impacted on the selected sample of urban students in the choice of their presidential candidates in the December 2001 elections in Zambia. The study further investigates to what extent other factors such as ethnic inclinations and other social predispositions influenced them in their choice of the candidates. The interviewees for this study were randomly drawn from a population of Evelyn Hone College students that voted in the December 2001 presidential elections in Zambia. The sample consisted of 30 randomly selected students who were purposely stratified in three focus groups of ten each. Each stratum represented the approximate ethnic equivalence of one of the presidential candidates who contested the December 2001 elections.
- Full Text:
- Date Issued: 2003
An object relational psychoanalysis of selected Tennessee Williams play texts
- Authors: Tosio, Paul
- Date: 2003
- Subjects: Williams, Tennessee, 1911-1983 Williams, Tennessee, 1911-1983 -- Knowledge -- Psychology Object relations (Psychoanalysis) Psychoanalysis Drama -- Psychological aspects
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2150 , http://hdl.handle.net/10962/d1002382
- Description: Tennessee Williams is a playwright of great psychological depth. This thesis probes some of the complexities of his work through the use of Object Relational Psychoanalysis, specifically employing the theories of Melanie Klein, W.R.D. Fairbairn and Donald Winnicott. The Glass Menagerie, A Streetcar Named Desire, Cat On A Hot Tin Roof and The Night of The Iguana are analysed from this theoretical stance. All of these plays display great perceptiveness into the human condition, accurately portraying many psychological relational themes. Certain Object Relational themes become very apparent in these analyses. These themes include, Dependency (especially in The Glass Menagerie), Reparation (particularly in A Streetcar Named Desire), Falsehood (notably in Cat on a Hot Tin Roof), Idealisation (evident in The Night of The Iguana), Honest Empathetic Relations (apparent in Cat on a Hot Tin Roof and The Night of The Iguana) as well as Guilt, Object Loss, Sexual Guilt, and Obligation (recurring throughout these plays). It is advanced that Williams’ plays posses an honest and insightful understanding of human relations and, as such, are of contemporary value. This Thesis is not only an academic study, but also has practical applications for dramatists. With an increased understanding of the intrinsic tensions and motivations within such plays, offered by such psychoanalytic strategy, performance and staging of such work may be enhanced valuably.
- Full Text:
- Date Issued: 2003
- Authors: Tosio, Paul
- Date: 2003
- Subjects: Williams, Tennessee, 1911-1983 Williams, Tennessee, 1911-1983 -- Knowledge -- Psychology Object relations (Psychoanalysis) Psychoanalysis Drama -- Psychological aspects
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2150 , http://hdl.handle.net/10962/d1002382
- Description: Tennessee Williams is a playwright of great psychological depth. This thesis probes some of the complexities of his work through the use of Object Relational Psychoanalysis, specifically employing the theories of Melanie Klein, W.R.D. Fairbairn and Donald Winnicott. The Glass Menagerie, A Streetcar Named Desire, Cat On A Hot Tin Roof and The Night of The Iguana are analysed from this theoretical stance. All of these plays display great perceptiveness into the human condition, accurately portraying many psychological relational themes. Certain Object Relational themes become very apparent in these analyses. These themes include, Dependency (especially in The Glass Menagerie), Reparation (particularly in A Streetcar Named Desire), Falsehood (notably in Cat on a Hot Tin Roof), Idealisation (evident in The Night of The Iguana), Honest Empathetic Relations (apparent in Cat on a Hot Tin Roof and The Night of The Iguana) as well as Guilt, Object Loss, Sexual Guilt, and Obligation (recurring throughout these plays). It is advanced that Williams’ plays posses an honest and insightful understanding of human relations and, as such, are of contemporary value. This Thesis is not only an academic study, but also has practical applications for dramatists. With an increased understanding of the intrinsic tensions and motivations within such plays, offered by such psychoanalytic strategy, performance and staging of such work may be enhanced valuably.
- Full Text:
- Date Issued: 2003
Analysis of material flow around a retractable pin in a friction stir weld
- Authors: Georgeou, Zacharias
- Date: 2003
- Subjects: Friction welding , Aluminum alloys -- Welding
- Language: English
- Type: Thesis , Masters , MTech (Mechanical Engineering)
- Identifier: vital:10812 , http://hdl.handle.net/10948/196 , Friction welding , Aluminum alloys -- Welding
- Description: Friction StirWelding (FSW) has been researched for a number of years since its inception in 1991. The work thus far has been based on understanding the material and thermal flow using the standard fixed pin tool. The keyhole resulting during tool extraction in a FSW weld, is a disadvantage and a current limiting factor. Eliminating this effect from a weld using a movable pin tools would make FSW more commercially viable. This dissertation focuses on the design of a novel retractable pin tool, and highlights the problems encountered during the welding of Aluminum plates, Al2024 and Al5083. Previously studied techniques of material and thermal flow were used, to investigate the effect of the tool during extraction in a FSW weld. A prototype retractable tool was designed using parametric and axiomatic design theory, and implementing a pneumatic muscle actuation system. The resulting problems in the calibration of the retractable pin tool and the resulting welds are presented, these results confirming previous studies. The movable pin produced discrepancies the heat generation around the shoulder during a FSW weld. The failure of this tool to produce a reasonable weld showed that previous ideas into the workings of a retractable pin tool requires further investigation, furthermore a fresh approach to the interpretation and understanding of the FSW weld process needs consideration.
- Full Text:
- Date Issued: 2003
- Authors: Georgeou, Zacharias
- Date: 2003
- Subjects: Friction welding , Aluminum alloys -- Welding
- Language: English
- Type: Thesis , Masters , MTech (Mechanical Engineering)
- Identifier: vital:10812 , http://hdl.handle.net/10948/196 , Friction welding , Aluminum alloys -- Welding
- Description: Friction StirWelding (FSW) has been researched for a number of years since its inception in 1991. The work thus far has been based on understanding the material and thermal flow using the standard fixed pin tool. The keyhole resulting during tool extraction in a FSW weld, is a disadvantage and a current limiting factor. Eliminating this effect from a weld using a movable pin tools would make FSW more commercially viable. This dissertation focuses on the design of a novel retractable pin tool, and highlights the problems encountered during the welding of Aluminum plates, Al2024 and Al5083. Previously studied techniques of material and thermal flow were used, to investigate the effect of the tool during extraction in a FSW weld. A prototype retractable tool was designed using parametric and axiomatic design theory, and implementing a pneumatic muscle actuation system. The resulting problems in the calibration of the retractable pin tool and the resulting welds are presented, these results confirming previous studies. The movable pin produced discrepancies the heat generation around the shoulder during a FSW weld. The failure of this tool to produce a reasonable weld showed that previous ideas into the workings of a retractable pin tool requires further investigation, furthermore a fresh approach to the interpretation and understanding of the FSW weld process needs consideration.
- Full Text:
- Date Issued: 2003
Aplousobranch ascidians (Tunicata: Ascidiacea) from Southern Africa
- Authors: Parker-Nance, Shirley
- Date: 2003
- Subjects: Sea squirts -- South Africa -- Classification , Aplousobranchia -- South Africa -- Classification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:11078 , http://hdl.handle.net/10948/311 , Sea squirts -- South Africa -- Classification , Aplousobranchia -- South Africa -- Classification
- Description: The ascidian (subphylum Tunicata: class Ascidiacea) fauna along the southern African coast constitutes an important component of the sessile benthic reef fauna. Little is known of the species composition, biodiversity and distribution of ascidians on southern African intertidal and subtidal reefs. Past research on the ascidian fauna of South Africa was sporadic and limited, with only about one hundred and seventy species recorded during the last one hundred and twenty years. This is the first taxonomic study undertaken by a South African resident scientist. The study focused on six genera in four families (Euherdmaniidae, Polyclinidae, Pseudodistomidae and Didemnidae) belonging to the class Ascidiacea and suborder Aplousobranchia. Five species new to science, two Polyclinum, two Pseudodistoma and one Polysyncraton species are described. New additional information on the distribution of five species previously known to science, including one recorded for the first time along the South African coast, is presented. A literature review and comparison of the taxonomic important characteristics is made of all species known globally for the six genera. These six genera comprise twelve Euherdmania, fifteen Aplidiopsis, forty Polyclinum thirty-one Pseudodistoma, six Atriolum and sixty-eight Polysyncraton species.
- Full Text:
- Date Issued: 2003
- Authors: Parker-Nance, Shirley
- Date: 2003
- Subjects: Sea squirts -- South Africa -- Classification , Aplousobranchia -- South Africa -- Classification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:11078 , http://hdl.handle.net/10948/311 , Sea squirts -- South Africa -- Classification , Aplousobranchia -- South Africa -- Classification
- Description: The ascidian (subphylum Tunicata: class Ascidiacea) fauna along the southern African coast constitutes an important component of the sessile benthic reef fauna. Little is known of the species composition, biodiversity and distribution of ascidians on southern African intertidal and subtidal reefs. Past research on the ascidian fauna of South Africa was sporadic and limited, with only about one hundred and seventy species recorded during the last one hundred and twenty years. This is the first taxonomic study undertaken by a South African resident scientist. The study focused on six genera in four families (Euherdmaniidae, Polyclinidae, Pseudodistomidae and Didemnidae) belonging to the class Ascidiacea and suborder Aplousobranchia. Five species new to science, two Polyclinum, two Pseudodistoma and one Polysyncraton species are described. New additional information on the distribution of five species previously known to science, including one recorded for the first time along the South African coast, is presented. A literature review and comparison of the taxonomic important characteristics is made of all species known globally for the six genera. These six genera comprise twelve Euherdmania, fifteen Aplidiopsis, forty Polyclinum thirty-one Pseudodistoma, six Atriolum and sixty-eight Polysyncraton species.
- Full Text:
- Date Issued: 2003
Apollo, Dionysus, dialectical reason and critical cinema
- Authors: Konik, Adrian
- Date: 2003
- Subjects: Motion pictures and language , Philosophy in motion pictures , Dionysus (Greek deity) in literature , Apollo (Greek deity) in literature
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:10990 , http://hdl.handle.net/10948/295 , Motion pictures and language , Philosophy in motion pictures , Dionysus (Greek deity) in literature , Apollo (Greek deity) in literature
- Description: The contemporary era is dominated by an Apollonian visual language, i.e. the visual language of mainstream cinema and the mass media, and this study concerns the role that critical cinema, as Dionysian subverter, plays under such conditions. I argue that critical cinema should not be viewed as something completely ‘new’ but rather as a new, or at least the latest, manifestation of an older subversive ‘Dionysian’ voice that has made its presence felt since the dawn of the hegemony of an Apollonian disposition in Homeric epic. (I maintain that the history of western culture can be understood in terms of the persistent tension between Apollonian and Dionysian dispositions, and I use the distinction Derrida makes in Différance, between restricted and general economies, to distinguish between them, respectively.) I begin by considering the Dionysian echoes within Homer’s Iliad and then consider the way in which they became a ‘roar’ in the tragedies of Aeschylus. After Aeschylus a predominantly Apollonian voice asserted itself once again (to various degrees) through the work of Sophocles and Euripides. This was in keeping with the trend towards a more (Apollonian) restricted economy that is reflected in the writings of Homer’s literary successors, and which reached a crucial stage in Plato’s valorisation of ‘dialectics’, or what I term ‘dialecticis m’, which saw the birth of ‘dialectical language’. Through Plato dialecticism, or dialectical language, became instantiated as the ‘language’ of western philosophy and this predisposed western culture to develop along predominantly Apollonian lines. This continued from Plato, through the Middle Ages, until in the 17th century this Apollonian trend became manifest in the concept of the stable, integral, autonomous and self -transparent Cartesian ego, which is inextricably linked to dialectical language that promises certainty of ‘truth’ and maintains the possibility of representing the world in its entirety (as a system). In the contemporary ‘age of a world picture’, the hegemonic (Apollonian) visual language of mainstream cinema and the mass media propagates and perpetuates the belief in the possibility of representing the world in its entirety through the image, and insofar as it caters to audiences’ needs for stability and certainty (of ‘truth’) through providing such ‘complete’ representations, shapes their subjectivity along the lines of the Cartesian ego. According to Baudrillard, in contemporary society and culture the hyperreal realm of visual language has become far more significant for individuals than their immediate, empirical experiences, and that, as a result, they are far less predisposed to discussion and reflection and far more prone to passive ‘watching’. Also, Adorno maintains that it is impossible to have a form of critical cinema because of the way in which features inherent to cinema predispose it towards being an ideological apparatus. However, if both Baudrillard and Adorno are correct then the future appears increasingly bleak as it involves nothing other than the continuation and propagation of the hegemony of the visual language of mainstream cinema and the mass media, with no possibility for critical resistance. I argue instead that critical cinema is possible because the move towards a more restricted economy, motivated by an Apollonian disposition, did not develop from Homer to the contemporary era without meeting Dionysian resistance. I trace the presence of a subversive Dionysian voice through Homer’s Iliad, through Aeschylus’ Prometheus Bound, and through Plato’s Dialogues, where it echoes in the sentiments of some of Plato’s interlocutors, such as Callicles. In addition, I maintain that a ‘Dionysian’ voice resonates through both Nietzsche’s and Heidegger’s respective criticisms of ‘dialectical language’ and the ‘validity’ of the Cartesian ego. I argue that critical cinema, particularly Aronofsky’s postmodern critical cinema, parallels their similar epistemological and ontological perspectives in the way in which it engages with the (Apollonian) visual language of mainstream cinema and the mass media, and thereby, potentially, facilitates a more porous and protean subjectivity.
- Full Text:
- Date Issued: 2003
- Authors: Konik, Adrian
- Date: 2003
- Subjects: Motion pictures and language , Philosophy in motion pictures , Dionysus (Greek deity) in literature , Apollo (Greek deity) in literature
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:10990 , http://hdl.handle.net/10948/295 , Motion pictures and language , Philosophy in motion pictures , Dionysus (Greek deity) in literature , Apollo (Greek deity) in literature
- Description: The contemporary era is dominated by an Apollonian visual language, i.e. the visual language of mainstream cinema and the mass media, and this study concerns the role that critical cinema, as Dionysian subverter, plays under such conditions. I argue that critical cinema should not be viewed as something completely ‘new’ but rather as a new, or at least the latest, manifestation of an older subversive ‘Dionysian’ voice that has made its presence felt since the dawn of the hegemony of an Apollonian disposition in Homeric epic. (I maintain that the history of western culture can be understood in terms of the persistent tension between Apollonian and Dionysian dispositions, and I use the distinction Derrida makes in Différance, between restricted and general economies, to distinguish between them, respectively.) I begin by considering the Dionysian echoes within Homer’s Iliad and then consider the way in which they became a ‘roar’ in the tragedies of Aeschylus. After Aeschylus a predominantly Apollonian voice asserted itself once again (to various degrees) through the work of Sophocles and Euripides. This was in keeping with the trend towards a more (Apollonian) restricted economy that is reflected in the writings of Homer’s literary successors, and which reached a crucial stage in Plato’s valorisation of ‘dialectics’, or what I term ‘dialecticis m’, which saw the birth of ‘dialectical language’. Through Plato dialecticism, or dialectical language, became instantiated as the ‘language’ of western philosophy and this predisposed western culture to develop along predominantly Apollonian lines. This continued from Plato, through the Middle Ages, until in the 17th century this Apollonian trend became manifest in the concept of the stable, integral, autonomous and self -transparent Cartesian ego, which is inextricably linked to dialectical language that promises certainty of ‘truth’ and maintains the possibility of representing the world in its entirety (as a system). In the contemporary ‘age of a world picture’, the hegemonic (Apollonian) visual language of mainstream cinema and the mass media propagates and perpetuates the belief in the possibility of representing the world in its entirety through the image, and insofar as it caters to audiences’ needs for stability and certainty (of ‘truth’) through providing such ‘complete’ representations, shapes their subjectivity along the lines of the Cartesian ego. According to Baudrillard, in contemporary society and culture the hyperreal realm of visual language has become far more significant for individuals than their immediate, empirical experiences, and that, as a result, they are far less predisposed to discussion and reflection and far more prone to passive ‘watching’. Also, Adorno maintains that it is impossible to have a form of critical cinema because of the way in which features inherent to cinema predispose it towards being an ideological apparatus. However, if both Baudrillard and Adorno are correct then the future appears increasingly bleak as it involves nothing other than the continuation and propagation of the hegemony of the visual language of mainstream cinema and the mass media, with no possibility for critical resistance. I argue instead that critical cinema is possible because the move towards a more restricted economy, motivated by an Apollonian disposition, did not develop from Homer to the contemporary era without meeting Dionysian resistance. I trace the presence of a subversive Dionysian voice through Homer’s Iliad, through Aeschylus’ Prometheus Bound, and through Plato’s Dialogues, where it echoes in the sentiments of some of Plato’s interlocutors, such as Callicles. In addition, I maintain that a ‘Dionysian’ voice resonates through both Nietzsche’s and Heidegger’s respective criticisms of ‘dialectical language’ and the ‘validity’ of the Cartesian ego. I argue that critical cinema, particularly Aronofsky’s postmodern critical cinema, parallels their similar epistemological and ontological perspectives in the way in which it engages with the (Apollonian) visual language of mainstream cinema and the mass media, and thereby, potentially, facilitates a more porous and protean subjectivity.
- Full Text:
- Date Issued: 2003
Applications of the Baylis-Hillman reaction in the synthesis of coumarin derivatives
- Authors: Musa, Musiliyu Ayodele
- Date: 2003
- Subjects: Coumarins Heterocyclic compounds -- Derivatives
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4403 , http://hdl.handle.net/10962/d1006705
- Description: The reaction of specially prepared salicylaldehyde benzyl ethers with the activated alkenes, methyl acrylate or acrylonitrile, in the presence of the catalyst, DABCO, has afforded Baylis-Hillman products, which have been subjected to conjugate addition with either piperidine or benzylamine. Hydrogenolysis of these conjugate addition products in the presence of a palladium-on-carbon catalyst has been shown to afford the corresponding 3-substituted coumarins, while treatment of O-benzylated Baylis-Hillman adducts with HCl or HI afforded the corresponding 3-(halomethyl)coumarins directly, in up to 94%. The 3-(halomethyl)coumarins have also been obtained in excellent yields (up to 98%) and even more conveniently, by treating the unprotected Baylis-Hillman products with HCl in a mixture of AcOH and Ac₂O, obtained from tert-butyl acrylate and various salicylaldehydes. The generality of an established route to the synthesis of coumarins via an intramolecular Baylis-Hillman reaction, involving the use of salicylaldehyde acrylate esters in the presence of DABCO, has also been demonstrated. Reactions between the 3-(halomethyl)coumarins and various nitrogen and carbon nucleophiles have been shown to proceed with a high degree of regioselectivity at the exocyclic allylic centre to afford 3-substituted coumarin products. The electronimpact mass spectra of selected coumarin derivatives have been investigated using high-resolution and B/E linked scan data. Fragmentation pathways have been proposed and fragmentation modes associated with different coumarin-containing analogues have been compared. A series of coumarin-containing analogues of ritonavir (a clinically useful HIV-1 protease inhibitor) have been prepared and characterized. The synthetic approach has involved the coupling of coumarin derivatives with a hydroxyethylene dipeptide isostere to afford ritonavir analogues containing coumarin termini. An interactive docking procedure has been used to explore the docking of ritonavir and a coumarincontaining analogue into the enzyme active site.
- Full Text:
- Date Issued: 2003
- Authors: Musa, Musiliyu Ayodele
- Date: 2003
- Subjects: Coumarins Heterocyclic compounds -- Derivatives
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4403 , http://hdl.handle.net/10962/d1006705
- Description: The reaction of specially prepared salicylaldehyde benzyl ethers with the activated alkenes, methyl acrylate or acrylonitrile, in the presence of the catalyst, DABCO, has afforded Baylis-Hillman products, which have been subjected to conjugate addition with either piperidine or benzylamine. Hydrogenolysis of these conjugate addition products in the presence of a palladium-on-carbon catalyst has been shown to afford the corresponding 3-substituted coumarins, while treatment of O-benzylated Baylis-Hillman adducts with HCl or HI afforded the corresponding 3-(halomethyl)coumarins directly, in up to 94%. The 3-(halomethyl)coumarins have also been obtained in excellent yields (up to 98%) and even more conveniently, by treating the unprotected Baylis-Hillman products with HCl in a mixture of AcOH and Ac₂O, obtained from tert-butyl acrylate and various salicylaldehydes. The generality of an established route to the synthesis of coumarins via an intramolecular Baylis-Hillman reaction, involving the use of salicylaldehyde acrylate esters in the presence of DABCO, has also been demonstrated. Reactions between the 3-(halomethyl)coumarins and various nitrogen and carbon nucleophiles have been shown to proceed with a high degree of regioselectivity at the exocyclic allylic centre to afford 3-substituted coumarin products. The electronimpact mass spectra of selected coumarin derivatives have been investigated using high-resolution and B/E linked scan data. Fragmentation pathways have been proposed and fragmentation modes associated with different coumarin-containing analogues have been compared. A series of coumarin-containing analogues of ritonavir (a clinically useful HIV-1 protease inhibitor) have been prepared and characterized. The synthetic approach has involved the coupling of coumarin derivatives with a hydroxyethylene dipeptide isostere to afford ritonavir analogues containing coumarin termini. An interactive docking procedure has been used to explore the docking of ritonavir and a coumarincontaining analogue into the enzyme active site.
- Full Text:
- Date Issued: 2003
Applied studies of some Southern African blowflies (Diptera: Calliphoridae) of forensic importance
- Authors: Lunt, Nicola
- Date: 2003
- Subjects: Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5803 , http://hdl.handle.net/10962/d1006202 , Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Description: Three major aspects of blowfly (Diptera: Calliphoridae) research were the focus of this study. Firstly, the phylogenetic relationships of 40 oestroid species from a variety of geographical localities were investigated using Cytochrome Oxidase b subunit I (COl) gene sequences. Maximum parsimony (MP) and Jukes-Cantor neighbor-joining (NJ) analyses both extracted a paraphyletic Calliphoridae, with the Calliphorinae-Luciliinae clade being sister to the Sarcophagidae. Short branch lengths within Chrysomya indicate a recent rapid radiation of this genus. Phormia and Protophormia either formed a sister clade to Chrysomya, or were embedded in this genus. Tree topologies were comparable between MP and NJ trees, but the positions of some genera were ambiguous. Secondly, developmental parameters and behaviour were investigated for four southern African species of forensically important blowflies viz. Chrysomya chloropyga, C. putoria, C. megacephala and Lucilia sericata, and ad hoc observations were made for Calliphora croceipalpis, Chrysomya marginalis and the predatory C. albiceps. Choice of oviposition substrate differed between species, mirroring substrate preferences in the field. Sexual dimorphism and dwarfism within a cohort complicated ageing maggots using size, but the use of developmental events (e.g. ecdysis) allowed ages to be determined unambiguously. Separate species status was supported for the previously synonymised C. chloropyga and C. putoria, by differences in maggot behaviour, larval growth rates and temperature optima. The proportion of total development time assigned to each larval instar and pupariation was variable among temperatures, but similar between congeneric species. Thirdly, since a negative linear relationship was found to occur between the developmental constant (K) and developmental zero (D₀) for both Calliphoridae and Sarcophagidae, the potential for predicting physiological parameters of unstudied taxa was investigated. Species and genera of Palaearctic origin generally had high K's and low D₀'s, and the reverse was true for the tropical taxa. It was found that both K and D₀ can be estimated for "unknown" taxa using the Felsenstein's Independent Contrasts (FIC) method of PDTree (Garland et al. 200 I), provided that branch lengths are relatively short and the phylogenetic position of the estimated taxon is unambiguous.
- Full Text:
- Date Issued: 2003
- Authors: Lunt, Nicola
- Date: 2003
- Subjects: Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5803 , http://hdl.handle.net/10962/d1006202 , Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Description: Three major aspects of blowfly (Diptera: Calliphoridae) research were the focus of this study. Firstly, the phylogenetic relationships of 40 oestroid species from a variety of geographical localities were investigated using Cytochrome Oxidase b subunit I (COl) gene sequences. Maximum parsimony (MP) and Jukes-Cantor neighbor-joining (NJ) analyses both extracted a paraphyletic Calliphoridae, with the Calliphorinae-Luciliinae clade being sister to the Sarcophagidae. Short branch lengths within Chrysomya indicate a recent rapid radiation of this genus. Phormia and Protophormia either formed a sister clade to Chrysomya, or were embedded in this genus. Tree topologies were comparable between MP and NJ trees, but the positions of some genera were ambiguous. Secondly, developmental parameters and behaviour were investigated for four southern African species of forensically important blowflies viz. Chrysomya chloropyga, C. putoria, C. megacephala and Lucilia sericata, and ad hoc observations were made for Calliphora croceipalpis, Chrysomya marginalis and the predatory C. albiceps. Choice of oviposition substrate differed between species, mirroring substrate preferences in the field. Sexual dimorphism and dwarfism within a cohort complicated ageing maggots using size, but the use of developmental events (e.g. ecdysis) allowed ages to be determined unambiguously. Separate species status was supported for the previously synonymised C. chloropyga and C. putoria, by differences in maggot behaviour, larval growth rates and temperature optima. The proportion of total development time assigned to each larval instar and pupariation was variable among temperatures, but similar between congeneric species. Thirdly, since a negative linear relationship was found to occur between the developmental constant (K) and developmental zero (D₀) for both Calliphoridae and Sarcophagidae, the potential for predicting physiological parameters of unstudied taxa was investigated. Species and genera of Palaearctic origin generally had high K's and low D₀'s, and the reverse was true for the tropical taxa. It was found that both K and D₀ can be estimated for "unknown" taxa using the Felsenstein's Independent Contrasts (FIC) method of PDTree (Garland et al. 200 I), provided that branch lengths are relatively short and the phylogenetic position of the estimated taxon is unambiguous.
- Full Text:
- Date Issued: 2003
Aspekte van die prosa van André P Brink met spesifieke verwysing na Sandkastele (1995)
- Authors: Vermeulen, Liezel
- Date: 2003
- Subjects: Brink, André P (André Philippus), 1935-2015 -- Sandkastele Brink, André P (André Philippus), 1935-2015
- Language: Afrikaans
- Type: Thesis , Masters , MA
- Identifier: vital:3605 , http://hdl.handle.net/10962/d1003809
- Description: Hierdie studie ondersoek aspekte van die prosa-oeuvre van die Suid-Afrikaanse outeur André P. Brink, met spesifieke verwysing na sy eerste post-apartheid roman Sandkastele (1995). Hierdie teks se feministiese hersiening van en alternatief tot die manlikgedomineerde en -gedokumenteerde amptelike geskiedenis van Suider-Afrika staan sentraal in die postmodernistiese, postkoloniale fase waarin die Brink prosa-oeuvre die verlede heroorweeg. Die pre-koloniale en koloniale verlede van die landstreek word herkaart deur die vertelling van stories van vroue van die verlede in 'n unieke vertelsituasie wat 'n her-verbeelde, tekstuele vrouegeskiedenis registreer. Die konsep "reimagination" wat ontwikkel in Brink se kritiese en literêr-teoretiese werke vanaf die publikasie van die essay "Imagining the real" in Mapmakers (1983) en Elleke Boehmer se konseptualisering van die tekstuele gevolge van dekolonisasie (1995) word ontgin. Spore van die magiese realisme, die postkolonialisme, die poststrukturalistiese postmodernisme en leeswyses en denkrigtings wat in die feminisme en genderstudies aandag geniet, word in Sandkastele se vertelling, narratiewe figure en ruimte ondersoek. Hierdie leesstrategie akkommodeer die Brink-oeuvre se voortdurende heroorweging en ontdekking van nuwe tekstuele moontlikhede, 'n proses wat gekenmerk word deur 'n reeks tematiese of konseptuele ontwikkelinge en die uit-en deurwerk van belangstellings van vorige Brinktekste. ABSTRACT: This study investigates aspects of the prose oeuvre of the South African author André P. Brink, with specific reference to his first post-apartheid novel Imaginings of Sand (1995). The feminist revisioning of the text as an alternative to the male-dominated and -documented official history of Southern Africa is central to the postmodernist, postcolonial phase of the Brink oeuvre in which the past is reconsidered. The pre-colonial and colonial past of the country is remapped through the stories of women of the past in a narrative construction which registers a re-imagined textual women's history. The concept "reimagination" which develops in Brink's critical and literary theoretical works from the publication of the essay "Imagining the real" in Mapmakers (1983) and Elleke Boehmer's conceptualisation of the textual effects of decolonisation (1995) is utilised. Traces of the magical realism, postcolonialism, poststructuralist postmodernism and approaches which are explored in feminism and gender studies are explored in Sandkastele's narrative construction, characters and space. This reading strategy accommodates the Brink oeuvre's continual re-evaluation and uncovering of new textual possibilities, a process that is characterised by a series of thematic or conceptual developments and the deployment of themes from previous Brink prose texts.
- Full Text:
- Date Issued: 2003
- Authors: Vermeulen, Liezel
- Date: 2003
- Subjects: Brink, André P (André Philippus), 1935-2015 -- Sandkastele Brink, André P (André Philippus), 1935-2015
- Language: Afrikaans
- Type: Thesis , Masters , MA
- Identifier: vital:3605 , http://hdl.handle.net/10962/d1003809
- Description: Hierdie studie ondersoek aspekte van die prosa-oeuvre van die Suid-Afrikaanse outeur André P. Brink, met spesifieke verwysing na sy eerste post-apartheid roman Sandkastele (1995). Hierdie teks se feministiese hersiening van en alternatief tot die manlikgedomineerde en -gedokumenteerde amptelike geskiedenis van Suider-Afrika staan sentraal in die postmodernistiese, postkoloniale fase waarin die Brink prosa-oeuvre die verlede heroorweeg. Die pre-koloniale en koloniale verlede van die landstreek word herkaart deur die vertelling van stories van vroue van die verlede in 'n unieke vertelsituasie wat 'n her-verbeelde, tekstuele vrouegeskiedenis registreer. Die konsep "reimagination" wat ontwikkel in Brink se kritiese en literêr-teoretiese werke vanaf die publikasie van die essay "Imagining the real" in Mapmakers (1983) en Elleke Boehmer se konseptualisering van die tekstuele gevolge van dekolonisasie (1995) word ontgin. Spore van die magiese realisme, die postkolonialisme, die poststrukturalistiese postmodernisme en leeswyses en denkrigtings wat in die feminisme en genderstudies aandag geniet, word in Sandkastele se vertelling, narratiewe figure en ruimte ondersoek. Hierdie leesstrategie akkommodeer die Brink-oeuvre se voortdurende heroorweging en ontdekking van nuwe tekstuele moontlikhede, 'n proses wat gekenmerk word deur 'n reeks tematiese of konseptuele ontwikkelinge en die uit-en deurwerk van belangstellings van vorige Brinktekste. ABSTRACT: This study investigates aspects of the prose oeuvre of the South African author André P. Brink, with specific reference to his first post-apartheid novel Imaginings of Sand (1995). The feminist revisioning of the text as an alternative to the male-dominated and -documented official history of Southern Africa is central to the postmodernist, postcolonial phase of the Brink oeuvre in which the past is reconsidered. The pre-colonial and colonial past of the country is remapped through the stories of women of the past in a narrative construction which registers a re-imagined textual women's history. The concept "reimagination" which develops in Brink's critical and literary theoretical works from the publication of the essay "Imagining the real" in Mapmakers (1983) and Elleke Boehmer's conceptualisation of the textual effects of decolonisation (1995) is utilised. Traces of the magical realism, postcolonialism, poststructuralist postmodernism and approaches which are explored in feminism and gender studies are explored in Sandkastele's narrative construction, characters and space. This reading strategy accommodates the Brink oeuvre's continual re-evaluation and uncovering of new textual possibilities, a process that is characterised by a series of thematic or conceptual developments and the deployment of themes from previous Brink prose texts.
- Full Text:
- Date Issued: 2003
Binding and transcriptional activation by Uga3p, a zinc binuclear cluster protein of Saccharomyces cerevisiae redefining the UAS [subscript GABA] and the Uga3p binding site
- Authors: Idicula, Anu Mary
- Date: 2003
- Subjects: Saccharomyces cerevisiae GABA
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3933 , http://hdl.handle.net/10962/d1003992
- Description: Uga3p, a member of the zinc binuclear cluster transcription factor family, is required for [gamma]-aminobutyric acid-dependent transcription of the UGA genes in Saccharomyces cerevisiae. Crystallographic data of some of the protein-DNA complexes of this family reveal that members of this family bind to CGG triplets. A conserved 19-nucleotide activation element in certain UGA gene promoter regions contains a CCG-N4-CGG everted repeat, proposed to be the binding site of Uga3p, UAS[subscript GABA]. The spacer region (N4) between the CGG triplets has been suggested to be the specificity determinant for binding to UAS[subscript GABA]. The data available from the Saccharomyces genome database indicates that there are multiple repeats of -CCG-N4-CGG- regions within the genome. These transcription factors are involved in the activation of specific pathways and the question arises as to how their specificity of binding is determined. The aim of this study was to understand the binding characteristics of Uga3p to UAS[subscript GABA] and to determine the affinity and specificity of this interaction. In this study, full-length (tagged and untagged) and truncated (1-124 a.a.) Uga3p was produced in a heterologous expression system (E. coli). The interaction of Uga3p with UAS[subscript GABA] in Saccharomyces cerevisiae was characterized in terms of binding in vitro and the transcriptional activation of lacZ reporter genes in vivo. The Uga3p was capable of binding to these sites in vitro independent of exogenous GABA. Electrophoretic mobility shift assays (EMSA) of the full-length Uga3p with the wild type UAS[subscript GABA] sequences produced two distinct mobility complexes. The complexes formed in the EMSA of the full-length Uga3p were those specific to the interaction of the Uga3p to UAS[subscript GABA]. The truncated Uga3p(1-124 a.a.), which has the DNA-binding zinc cluster domain, the linker region and the putative coiled-coil domain was not functionally equivalent to the full-length protein with respect to binding in vitro because the EMSAs of the UAS[subscript GABA] with the truncated Uga3p produced indistinct complexes. EMSAs using mutant UAS[subscript GABA] sequences and heterologously-produced full-length Uga3p, demonstrated that UAS[subscript GABA] consists of two, independent Uga3p-binding sites. This work presents evidence that the two Uga3p molecules bound to UAS[subscript GABA] most likely interact with each other. Unlike other zinc cluster binding sites the Uga3p-binding site is an asymmetric site of 5’-SGCGGNWWT-3’ (S= G or C, W = A or T and N = no nucleotide or G or C). UAS[subscript GABA] is a palindrome containing the two asymmetric Uga3p-binding sites. The two-site consensus sequence required for the binding of Uga3p to the UAS[subscript GABA] is present upstream of UGA1 (region -387 to -370) and UGA4 (region -403 to -387). Furthermore, a single Uga3p-binding site was identified in the 5’ untranslated regions of UGA2 (region -219 to -211). GABA-dependent transcriptional activation by UAS[subscript GABA] in vivo could be directly correlated to a high affinity, specific interaction of two Uga3p molecules to this UAS. Binding with high affinity required the conserved sequences flanking the everted repeat. This study provided evidence that the binding pattern of Uga3p is novel compared to other zinc cluster motifs investigated, as the sequences flanking the everted repeat are important regions for recognition by Uga3p. The studies with the truncated Uga3p (1 –124 a.a.), also suggested that the regions C-terminal to the DNA-binding motif and putative coiled-coil area of this protein are important for Uga3p-specific interactions with UAS[subscript GABA]. Investigation of regions C-terminal to the zinc cluster, linker and putative coiledcoil revealed an eight-motif regulatory region similar to that in other zinc cluster proteins. This indicated that the regions C-terminal to these domains are important for the regulation and activity of these proteins. A putative seven repeat WD40-like motif was identified within this region. This putative domain has been speculated to be important for protein-protein interactions. Phosphorylation and dephosphorylation in other proteins of this class have been indicated to be important for the regulation of the activity of these proteins. The bioinformatic analysis of Uga3p revealed two possible cAMP/cGMP-dependent protein kinase phosphorylation sites, four putative protein kinase C phosphorylation motifs and four putative casein kinase II phosphorylation motifs. This study has contributed to the understanding of the nature of interactions between Uga3p and its specific UAS [subscript GABA] and how the regions flanking the everted repeat determine its specificity. The comparison of the nature of the binding of truncated and full-length Uga3p in vitro provided evidence for the role played by the full-length protein in determining this specific interaction. This evidence suggested that the in vitro binding evidence for other proteins of this family, using truncated peptides that carry the DNA-binding domain, might not reflect the true nature of interactions between the proteins of this class and their specific UASs in vivo.
- Full Text:
- Date Issued: 2003
- Authors: Idicula, Anu Mary
- Date: 2003
- Subjects: Saccharomyces cerevisiae GABA
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3933 , http://hdl.handle.net/10962/d1003992
- Description: Uga3p, a member of the zinc binuclear cluster transcription factor family, is required for [gamma]-aminobutyric acid-dependent transcription of the UGA genes in Saccharomyces cerevisiae. Crystallographic data of some of the protein-DNA complexes of this family reveal that members of this family bind to CGG triplets. A conserved 19-nucleotide activation element in certain UGA gene promoter regions contains a CCG-N4-CGG everted repeat, proposed to be the binding site of Uga3p, UAS[subscript GABA]. The spacer region (N4) between the CGG triplets has been suggested to be the specificity determinant for binding to UAS[subscript GABA]. The data available from the Saccharomyces genome database indicates that there are multiple repeats of -CCG-N4-CGG- regions within the genome. These transcription factors are involved in the activation of specific pathways and the question arises as to how their specificity of binding is determined. The aim of this study was to understand the binding characteristics of Uga3p to UAS[subscript GABA] and to determine the affinity and specificity of this interaction. In this study, full-length (tagged and untagged) and truncated (1-124 a.a.) Uga3p was produced in a heterologous expression system (E. coli). The interaction of Uga3p with UAS[subscript GABA] in Saccharomyces cerevisiae was characterized in terms of binding in vitro and the transcriptional activation of lacZ reporter genes in vivo. The Uga3p was capable of binding to these sites in vitro independent of exogenous GABA. Electrophoretic mobility shift assays (EMSA) of the full-length Uga3p with the wild type UAS[subscript GABA] sequences produced two distinct mobility complexes. The complexes formed in the EMSA of the full-length Uga3p were those specific to the interaction of the Uga3p to UAS[subscript GABA]. The truncated Uga3p(1-124 a.a.), which has the DNA-binding zinc cluster domain, the linker region and the putative coiled-coil domain was not functionally equivalent to the full-length protein with respect to binding in vitro because the EMSAs of the UAS[subscript GABA] with the truncated Uga3p produced indistinct complexes. EMSAs using mutant UAS[subscript GABA] sequences and heterologously-produced full-length Uga3p, demonstrated that UAS[subscript GABA] consists of two, independent Uga3p-binding sites. This work presents evidence that the two Uga3p molecules bound to UAS[subscript GABA] most likely interact with each other. Unlike other zinc cluster binding sites the Uga3p-binding site is an asymmetric site of 5’-SGCGGNWWT-3’ (S= G or C, W = A or T and N = no nucleotide or G or C). UAS[subscript GABA] is a palindrome containing the two asymmetric Uga3p-binding sites. The two-site consensus sequence required for the binding of Uga3p to the UAS[subscript GABA] is present upstream of UGA1 (region -387 to -370) and UGA4 (region -403 to -387). Furthermore, a single Uga3p-binding site was identified in the 5’ untranslated regions of UGA2 (region -219 to -211). GABA-dependent transcriptional activation by UAS[subscript GABA] in vivo could be directly correlated to a high affinity, specific interaction of two Uga3p molecules to this UAS. Binding with high affinity required the conserved sequences flanking the everted repeat. This study provided evidence that the binding pattern of Uga3p is novel compared to other zinc cluster motifs investigated, as the sequences flanking the everted repeat are important regions for recognition by Uga3p. The studies with the truncated Uga3p (1 –124 a.a.), also suggested that the regions C-terminal to the DNA-binding motif and putative coiled-coil area of this protein are important for Uga3p-specific interactions with UAS[subscript GABA]. Investigation of regions C-terminal to the zinc cluster, linker and putative coiledcoil revealed an eight-motif regulatory region similar to that in other zinc cluster proteins. This indicated that the regions C-terminal to these domains are important for the regulation and activity of these proteins. A putative seven repeat WD40-like motif was identified within this region. This putative domain has been speculated to be important for protein-protein interactions. Phosphorylation and dephosphorylation in other proteins of this class have been indicated to be important for the regulation of the activity of these proteins. The bioinformatic analysis of Uga3p revealed two possible cAMP/cGMP-dependent protein kinase phosphorylation sites, four putative protein kinase C phosphorylation motifs and four putative casein kinase II phosphorylation motifs. This study has contributed to the understanding of the nature of interactions between Uga3p and its specific UAS [subscript GABA] and how the regions flanking the everted repeat determine its specificity. The comparison of the nature of the binding of truncated and full-length Uga3p in vitro provided evidence for the role played by the full-length protein in determining this specific interaction. This evidence suggested that the in vitro binding evidence for other proteins of this family, using truncated peptides that carry the DNA-binding domain, might not reflect the true nature of interactions between the proteins of this class and their specific UASs in vivo.
- Full Text:
- Date Issued: 2003
Bioaccumulation and histopathology of copper in Oreochromis mossambicus
- Authors: Naigaga, Irene
- Date: 2003
- Subjects: Mozambique tilapia , Copper , Marine toxins , Fishes -- Effect of water pollution on , Water -- Pollution -- Environmental aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5234 , http://hdl.handle.net/10962/d1005077 , Mozambique tilapia , Copper , Marine toxins , Fishes -- Effect of water pollution on , Water -- Pollution -- Environmental aspects
- Description: Cu is one of the most toxic elements that affect fish populations when the fish are exposed to concentrations exceeding their tolerance. To investigate the effects of elementary Cu on aspects of bioconcentration, histology and behaviour, O. mossambicus were exposed to 0 and 0.75 ± 0.20 mg/l of Cu for 96 hours (short-term study), and 0, 0.11 ± 0.02, 0.29 ± 0.02, and 0.47 ± 0.04 mg/l of Cu for 64 days (longterm study) under controlled conditions in the laboratory. For the long-term study fish were sampled for gills, liver, and kidney Cu accumulation analysis after 1, 32 and 64 days of exposure and after 1, 2, 4, 16, 32, and 64 days for gills, liver and spleen histology analysis. Cu accumulation was concentration-duration dependent with the highest accumulation capacity in the liver. A multifactor linear model was developed for the relationship between exposure dose, exposure duration and Cu accumulation in the organs with the liver model: Log L = 3.35 + 0.85W + 0.31T (r² = 0.892) giving a better fit than the gills: G = −35.09 + 10.58W + 17.58T (r² = 0.632). Where L = Cu accumulation values in the liver, G = Cu accumulation values in the gills (both in μg/g dry mass); W = exposure dose in water (mg/l); and T = exposure time (days). Using this model Cu accumulation in organs can be estimated when exposure concentration and duration is known. This model should be tested under different conditions to determine the potential of the model in monitoring Cu toxicity in the environment. Lesions were observed in the liver, gills and spleen in all Cu treatments at all exposure concentration and exposure durations. However, the incidence and the degree of alteration was related to the concentration of Cu and duration of exposure. The sequential appearance of lesions in the order of, hepatic vacuolar degeneration, fatty degeneration and necrosis indicated a gradual increase in liver damage with larger duration of exposure time and increasing Cu concentration. The initial lesions in the gills were manifested as hypertrophy and hyperplasia of the gill epithelium causing increase in the thickness of the secondary lamellae, mucous cell hypertrophy and proliferation, mucous hypersecretion, proliferation of eosinophilic granule cells and hyperplasia of interlamellar cells. With increase in exposure time, necrosis of the eosinophilic granule cells, lamellar oedema, epithelial desquamation and increase in severity of lamellar hyperplasia were observed. These lesions indicated an initial defence mechanism of the fish against Cu toxicity followed by advanced histological changes that were related to Cu concentration and duration of exposure. Changes in the spleen were haemosiderosis, increase in the white pulp and macrophage centres, reduction in the red pulp, and necrosis suggesting that fish exposed to environmentally relevant levels of Cu may be histopathologically altered leading to anaemia and immunosuppression. Regression analysis was used to quantify the relationship between the total activity of the fish, and duration of exposure. There was a gradual decline in fish activity related to Cu concentration and duration of exposure before introducing food into the tanks. There was a constant activity after introducing food in the tanks at the control and 0.11 ± 0.02 mg/l Cu exposure levels irrespective of exposure time. Analysis of covariance (ANCOVA) was used to test for the difference in slopes between treatments. There was no significant difference (p > 0.05) between slopes of the control and 0.11 ± 0.02 mg/l Cu, and between 0.29 ± 0.02 and 47 ± 0.04 mg/l Cu before and after introducing food in the tanks. The slopes of both the control and 0.11 ± 0.02 mg/l Cu were significantly different from those of 0.29 ± 0.02 and 0.47 ± 0.04 mg/l Cu (p < 0.05). There were significant differences in the mean opercular movements per minute between treatments (p < 0.05). There was hyperventilation at 0.11 ± 0.02 mg/l Cu i.e. 87 ± 18 opercular movements per minute (mean ± standard deviation) and hypoventilation at 0.29 ± 0.02 and 0.47 ± 0.04 mg/l Cu i.e. 37 ± 34 and 13 ± 6 opercular movements per minute compared to the control. Hypo- and hyperventilation were related to the lesser and greater gill damage, respectively. In conclusion Cu accumulation and effects on histology of the liver, gills and were related to the concentration of Cu in the water and duration of exposure showing a gradual increase in incidence and intensity with larger duration of exposure time and increasing Cu concentration. The fish were initially able to homeostatically regulate and detoxify Cu. However, as the exposure continued, the homeostatic mechanism appears to have failed to cope with the increasing metal burden causing advanced histological changes.
- Full Text:
- Date Issued: 2003
- Authors: Naigaga, Irene
- Date: 2003
- Subjects: Mozambique tilapia , Copper , Marine toxins , Fishes -- Effect of water pollution on , Water -- Pollution -- Environmental aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5234 , http://hdl.handle.net/10962/d1005077 , Mozambique tilapia , Copper , Marine toxins , Fishes -- Effect of water pollution on , Water -- Pollution -- Environmental aspects
- Description: Cu is one of the most toxic elements that affect fish populations when the fish are exposed to concentrations exceeding their tolerance. To investigate the effects of elementary Cu on aspects of bioconcentration, histology and behaviour, O. mossambicus were exposed to 0 and 0.75 ± 0.20 mg/l of Cu for 96 hours (short-term study), and 0, 0.11 ± 0.02, 0.29 ± 0.02, and 0.47 ± 0.04 mg/l of Cu for 64 days (longterm study) under controlled conditions in the laboratory. For the long-term study fish were sampled for gills, liver, and kidney Cu accumulation analysis after 1, 32 and 64 days of exposure and after 1, 2, 4, 16, 32, and 64 days for gills, liver and spleen histology analysis. Cu accumulation was concentration-duration dependent with the highest accumulation capacity in the liver. A multifactor linear model was developed for the relationship between exposure dose, exposure duration and Cu accumulation in the organs with the liver model: Log L = 3.35 + 0.85W + 0.31T (r² = 0.892) giving a better fit than the gills: G = −35.09 + 10.58W + 17.58T (r² = 0.632). Where L = Cu accumulation values in the liver, G = Cu accumulation values in the gills (both in μg/g dry mass); W = exposure dose in water (mg/l); and T = exposure time (days). Using this model Cu accumulation in organs can be estimated when exposure concentration and duration is known. This model should be tested under different conditions to determine the potential of the model in monitoring Cu toxicity in the environment. Lesions were observed in the liver, gills and spleen in all Cu treatments at all exposure concentration and exposure durations. However, the incidence and the degree of alteration was related to the concentration of Cu and duration of exposure. The sequential appearance of lesions in the order of, hepatic vacuolar degeneration, fatty degeneration and necrosis indicated a gradual increase in liver damage with larger duration of exposure time and increasing Cu concentration. The initial lesions in the gills were manifested as hypertrophy and hyperplasia of the gill epithelium causing increase in the thickness of the secondary lamellae, mucous cell hypertrophy and proliferation, mucous hypersecretion, proliferation of eosinophilic granule cells and hyperplasia of interlamellar cells. With increase in exposure time, necrosis of the eosinophilic granule cells, lamellar oedema, epithelial desquamation and increase in severity of lamellar hyperplasia were observed. These lesions indicated an initial defence mechanism of the fish against Cu toxicity followed by advanced histological changes that were related to Cu concentration and duration of exposure. Changes in the spleen were haemosiderosis, increase in the white pulp and macrophage centres, reduction in the red pulp, and necrosis suggesting that fish exposed to environmentally relevant levels of Cu may be histopathologically altered leading to anaemia and immunosuppression. Regression analysis was used to quantify the relationship between the total activity of the fish, and duration of exposure. There was a gradual decline in fish activity related to Cu concentration and duration of exposure before introducing food into the tanks. There was a constant activity after introducing food in the tanks at the control and 0.11 ± 0.02 mg/l Cu exposure levels irrespective of exposure time. Analysis of covariance (ANCOVA) was used to test for the difference in slopes between treatments. There was no significant difference (p > 0.05) between slopes of the control and 0.11 ± 0.02 mg/l Cu, and between 0.29 ± 0.02 and 47 ± 0.04 mg/l Cu before and after introducing food in the tanks. The slopes of both the control and 0.11 ± 0.02 mg/l Cu were significantly different from those of 0.29 ± 0.02 and 0.47 ± 0.04 mg/l Cu (p < 0.05). There were significant differences in the mean opercular movements per minute between treatments (p < 0.05). There was hyperventilation at 0.11 ± 0.02 mg/l Cu i.e. 87 ± 18 opercular movements per minute (mean ± standard deviation) and hypoventilation at 0.29 ± 0.02 and 0.47 ± 0.04 mg/l Cu i.e. 37 ± 34 and 13 ± 6 opercular movements per minute compared to the control. Hypo- and hyperventilation were related to the lesser and greater gill damage, respectively. In conclusion Cu accumulation and effects on histology of the liver, gills and were related to the concentration of Cu in the water and duration of exposure showing a gradual increase in incidence and intensity with larger duration of exposure time and increasing Cu concentration. The fish were initially able to homeostatically regulate and detoxify Cu. However, as the exposure continued, the homeostatic mechanism appears to have failed to cope with the increasing metal burden causing advanced histological changes.
- Full Text:
- Date Issued: 2003