A possible mechanism for enzymic depilation of skins
- Authors: Brady, Dean
- Date: 1989
- Subjects: Chemistry, Technical , Tanning
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3877 , http://hdl.handle.net/10962/d1001611
- Description: Streptomyces fradiae is a bacterium which has been previously found to produce extracellular enzymes which are capable of wool degradation and skin depilation. Streptomyces fradiae 3739 and other strains of Streptomyces were found in this study to be able to degrade a keratin source (wool) to a considerable degree. However according to the evidence of SEM micrographs presented here the highly keratinised spindle cells of the paracortex are fairly resistant to protease attack, and it is the cementation material which binds these cells together which is initially degraded by the proteases. A large degree of correlation was found with the strains of Streptomyces studied, between the ability of the individual strains to degrade wool and the ability of their extracellular proteases to reduce the depilation load of sheepskins. With further analysis S. fradiae 3739 was found to produce at least one amylase and four or more proteases. The proteases as a group had maximal proteolytic activity in the 8.0-9.0 pH unit range, and were considerably thermostabilised by the inclusion of calcium ions into the reaction solution. The protease group was found to cause depilation of merino sheepskins. For comparative purposes a protease produced by a strain of Proteus vulgaris isolated from a staling hide with hair slip (natural depilation) was studied. The protease activity was maximal in the alkaline region between 8.0-9.0 pH units. Tbe protease appeared to be a single enzyme with a molecular mass of approximately 44 000 daltons. The protease was maximally active at 40°C, although it was only thermostable to 30°C. The enzyme was ineffectual as a depilant except when the skin was pre-treated with a strong alkali, preferably including sodium sulphite in the protease preparation. One of the most important differences between the extracellular proteases of S. fradiae and P. vulgaris was that the former were greater in variety and caused a greater decrease in the depilation load of sheepskins than the latter. Further research with mixtures of commercial proteases provided evidence that a synergistic depilatory effect occurs when proteases of complementary bond specificities are used in conjunction in enzymic depilatory preparations. Some form of strong alkali treatment of skins was found to be necessary to produce leather of the prerequisite quality when the skin was depilated by proteases, otherwise the skin was found to be depleted and stiff. Calcium hydroxide alone was found to be inadequate for this task, probably owing to the fact that it is less alkaline than the lime-sulphide mixture. The calcium hydroxide (lime) must therefore be used in conjunction with sodium hydroxide (which makes the solution as alkaline as that of the lime-sulphide solution) to produce leather comparable to that produced by the lime sulphide treatment. A combination of the information provided by the present research and that gleaned from the relevent literature allows for the construction of a model to represent the possible mechanism of enzymic depilation of skins, in which depilation is caused by the disruption of the basement membrane at the dermal-epidermal junction by the degradation of its constituent molecular components by general proteases, resulting in the removal of the epidermis and its associated wool or hair
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- Date Issued: 1989
A study on white leather tannage
- Authors: Williams-Wynn, D A
- Date: 1950
- Subjects: Leather , Tanning , Aluminum sulfate , Mineral tanning
- Language: English
- Type: Diploma theses , text
- Identifier: http://hdl.handle.net/10962/193713 , vital:45389
- Description: As has been noted in the previous section little or no complex formation occurs with either formate or acetate except possibly above pH 9*0 or so. These anions do not stabilise the aluminium solutions to precipitation - they may* however, be of value in controlling pH, as solutions of these salts buffer strongly in the region pH 4 to 5. The other salts, lactate, tartrate and citrate all show tendencies to complex formation though the degree to which this occurs varies with the anion. The complexes formed by lactate at high pH must be very unstable because on the addition of acid the pH corresponds to the calculated blank. Below pH 9.0 however, the curves deviate quite considerably showing that more acid must be added to reach a certain pH value than is required by the blank. This means complex formation has occurred with loss of -COGH groups. In this system too, buffering is apparent. The same is true of tartrate but this salt is not as efficient a buffering agent. This is probably due to the fact that the excess tartrate is not available as a buffer as it is precipitated as the acid salt which is only sparingly soluble. Citrate on the other hand must form very stable complexes, the effect of which is apparent at even very high pH values. In other words, addition of strong acid liberates free -GOGH groups which are immediately incorporated in the complex. This evidently does not occur with lactate or tartrate until lower pH values are reached. The ratio of lactate and citrate to aluminium appears to have some influence on the degree of masking as greater complex formation is found to occur at the higher ratio levels. Tartrate on the other hand apparently has a fixed complex forming capacity between 1 and 4 mols/mol aluminium, since the same deviation from the blank was observed irrespective of which ratio of the masking agent to aluminium, was employed. However, at ratios of less than 1 mol tartrate/mol aluminium, deviations from the blank were reduced and less stabilisation occurs as evidenced by the formation of precipitates containing aluminium under these conditions. In view of these facts it may be suggested that 1 mol tartrate combines with 1 mol aluminium to form a complex containing equimolar proportions of aluminium and tartrate. The effect of boiling apart from one or two exceptions always has the same effect, i.e. a shift to the acid side of the curve. Free acid must have been liberated which is an indication that olation might have occurred. In the lactate and citrate series dilution appears to reduce the complex forming capacity of these ions but tartrate is unaffected. Thus it would appear that the tartrate complex is more stable than those formed by lactate. Citrate stabilises aluminium solutions with fewer equivalents than tartrate, but the complex does not contain a constant ratio of masking agent to aluminium further quantities of tartrate cannot enter the complex even in the presence of large excess of the anion probably due to the effect of steric hindrance. , Thesis (Dip) -- Leather Industries Research Institute, 1950
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- Date Issued: 1950
Accelerated carbon dioxide deliming of cattle hides and sheepskins
- Authors: Flowers, Karl Bernard
- Date: 2002
- Subjects: Tanning , Hides and skins , Carbon dioxide
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3918 , http://hdl.handle.net/10962/d1003977 , Tanning , Hides and skins , Carbon dioxide
- Description: To avoid environmental pressure from water authorities, specifically regarding nitrogen and sulfate limits in tannery wastewater, modifications to existing deliming processes have been made. Conventional ammonium salt deliming methods contribute to Total Kjeldahl Nitrogen values in the region of 0.5 – 1.0g/L (33-67% of total TKN). Sulfate levels are increased with the use of organic deliming and ammonium sulfate deliming to the extent of 0.9g/L (27% of total sulfate). To understand the dynamics and kinetics of carbon dioxide equilibrium, the movement of carbon dioxide into deliming water, through carbonic acid, bicarbonate and ultimately into carbonates at liming or early deliming pH was studied. It was shown in this study that effective lime removal, at optimum conditions, resulted in fully delimed pelts at highly comparable quality and times compared to conventional ammonium salt deliming
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- Date Issued: 2002