Photosynthetic and growth response of C₃ and C₄ subspecies of Alloteropsis semialata to nitrogen-supply
- Authors: Abraham, Trevor Ian
- Date: 2008
- Subjects: Photosynthesis , Plants -- Effect of nitrogen on , Growth (Plants) , Plant ecology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4182 , http://hdl.handle.net/10962/d1003750 , Photosynthesis , Plants -- Effect of nitrogen on , Growth (Plants) , Plant ecology
- Description: The greater photosynthetic nitrogen use efficiency (PNUE) of C4 compared with C3 plants may explain the relative success of C4 grasses in nutrient poor environments. This study compared the responses in photosynthetic parameters, leaf nitrogen and biomass allocation between the C3 and C4 subspecies of Alloteropsis semialata supplied soil nitrogen at three levels. Photosynthesis was assessed by means of CO2 response curves and the leaf nitrogen content assayed. Plants were destructively harvested, leaf areas determined and the dry biomass of functional plant components was measured. Results confirmed that the higher PNUE of C4 plants allowed them to accumulate more biomass than C3 plants at the high nitrogen level, despite smaller leaf areas. The greater productivity of C4 plants enabled them to invest more in storage and sexual reproduction than in leaves when compared to the C3 plants. In contrast the C3 plants invested biomass in less efficient and more nitrogen demanding leaves and bigger root systems. PNUE and photosynthetic rates were not significantly affected by nitrogen-limitation in either subspecies and the major response was a decrease in biomass accumulation and an increase in biomass allocation to roots. This altered root to shoot ratio was accompanied by a lowered allocation to sexual reproduction in the C4 subspecies, but an unaltered allocation to leaves, while in the C3 subspecies there was a decrease in leaf allocation. In a further experiment, the C4 subspecies was supplied three levels of nitrogen provided as nitrate, or alternatively as ammonium plus nitrate, and leaves were excised to within 5 cm of the ground at the start of treatment. Prior to flowering, photosynthesis was assessed by means of CO2 response curves and the plants were destructively harvested. Leaf areas and the dry biomass of functional plant components were determined, and at levels of nitrogen supply higher than those found in savanna soils the rate of photosynthesis was increased. Leaf re-growth was reduced by severe nitrogen limitation and co-provision of nitrate and ammonium had no significant effect other than increased tillering. Both subspecies of Alloteropsis semialata are adapted to nutrient poor environments and maintain photosynthetic rates by reducing leaf area. The C4 subspecies is likely to show greater resilience in disturbance-prone environments by exploiting its higher PNUE to allocate greater resources to storage and sexual reproduction, while the C3 subspecies is usually found in environments with closed canopies which favour vegetative growth, and allocate greater resources to leaves and roots.
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Isolation and characterization of antiplasmodial metabolites from South African marine alga
- Authors: Afolayan, Anthonia Folake
- Date: 2008
- Subjects: Malaria -- Africa Antimalarials -- Therapeutic use Malaria -- Prevention Malaria -- Drug therapy Marine algae -- Therapeutic use Natural products -- Therapeutic use Plasmodium
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3739 , http://hdl.handle.net/10962/d1003063
- Description: Malaria is one of the three most deadly diseases in Africa. Although there are available treatments, their efficacy has been greatly reduced over the past two decades due to the development of resistance to currently available drugs. This has necessitated the search for new and effective antimalarial agents. This project approached the search for new antimalarial compounds in two ways: (i) by screening natural products isolated from marine algae against the Plasmodium parasite and (ii) by modification of selected isolated active compounds to target 1-deoxY-đ-xylulose 5-phosphate reductoisomerase (DXR), an enzyme found in the nonmevalonate isoprenoid biosynthetic pathway of Plasmodium Jalciparum. It was envisaged that such a compound would exhibit dual action on the Plasmodium parasite. Extracts obtained from 22 marine algae were prefractionated by solvent partitioning and were screened for anti plasmodial activity against the chloroquine sensitive (CQS) P. Jalciparum D 10 strain. Overall, 50% of the algae screened produced at least one crude fraction with activity against P. Jalciparum. Extracts of the algae Sargassum heterophyllum, Plocamium cornutum, Amphiroa ephedrea and Pterosiphonia cloiophylla gave the most promising results. Fractionation of S. heterophyllum afforded three tetraprenyltoluquinols (3.1, 3.2 and 3.5) and an all-trans-fucoxanthin (3.6). Three new compounds (4.5, 4.6 and 4.7) and two known halogenated monoterpenes (4.1 and 4.4) were isolated from P. cornutum. Each of the isolated compounds from both S. heterophyllum and P. cornutum showed antiplasmodial activity with IC₅₀ values ranging from 2.0 - 15.3 μM for S. heterophyllum and 13 - 230 μM for P. cornutum. Attempts to synthetically modify halogenated monoterpene 4.4 by dihydroxylation and phosphorylation in order to inhibit the DXR enzyme was unsuccessful. However, the hemiterpene analogue (5.42) of the halogenated monoterpenes was successfully phosphorylated and dihydroxylated to give compound 5.45 which showed promising activity against DXR. The result obtained indicated that the proposed phosphorylation and dihydroxylation of the halogenated monoterpene 4.4 would result in the synthesis of a potent DXR inhibitor and therefore a potential antimalarial agent with dual mode of action on the Plasmodium parasite.
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Ecological role of free-living bacteria in the microbial food web of the temporarily open/closed East Kleinemonde Estuary, South Africa
- Authors: Allan, Elizabeth Louise
- Date: 2008
- Subjects: Bacterial growth -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape , Estuaries -- South Africa -- Eastern Cape , Microbial ecology -- South Africa -- Eastern Cape , Nutrient cycles -- South Africa -- Eastern Cape , Food chains (Ecology)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5666 , http://hdl.handle.net/10962/d1005351 , Bacterial growth -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape , Estuaries -- South Africa -- Eastern Cape , Microbial ecology -- South Africa -- Eastern Cape , Nutrient cycles -- South Africa -- Eastern Cape , Food chains (Ecology)
- Description: The main aim of this study was to assess the “top-down” and “bottom-up” control of bacterial production in the small temporarily open/closed East Kleinemonde Estuary, situated on the south-eastern coastline of southern Africa. Spatial and temporal patterns in bacterial abundance, biomass and production and the importance of abiotic and biotic factors were investigated over the period May 2006 to April 2007. The trophic interactions between bacteria, phytoplankton, nanoflagellates (< 20 μm), microzooplankton (< 200 μm) and mesozooplankton (< 2 000 μm) were investigated during winter and summer. Bacterial abundance, biomass and production ranged between 1.00 × 10⁹ and 4.93 × 10⁹ cells 1⁻¹, 32.4 and 109 μg C 1⁻¹ and 0.01 and 1.99 μg C 1⁻¹ h⁻¹, respectively. With a few exceptions there were no spatial patterns in the values. Bacterial abundance, biomass and production, however, demonstrated a distinct temporal pattern with the lowest values consistently recorded during the winter months. Nanoflagellate and bacterial abundances were significantly correlated to one another (lower reaches: r = 0.818, p < 0.001; middle reaches: r = 0.628, p < 0.001; upper reaches: r = 0.484, p < 0.05) suggesting a strong predator-prey relationship. The frequency of visibly infected bacterial cells and the mean number of virus particles within each bacterial cell during this study demonstrated no temporal or spatial patterns and ranged from 0.5 to 6.1 % and 12.0 to 37.5 virus particles per bacterium, respectively. Viral infection and lysis was thus a constant source of bacterial mortality throughout the year. The estimated percentage of bacterial production removed by viral lysis ranged between 7.8 and 88.9% of the total which suggests that viral lysis represented a very important source of bacterial mortality during this study. The biological interactions between the selected components of the plankton community demonstrated that among the heterotrophic components of the plankton, the nanoflagellates were identified as the most important consumers of bacteria and small phytoplankton cells (< 20 μm). In the presence of microzooplankton the impact of the nanoflagellates on both the bacteria and phytoplankton was reduced, indicating that larger heterotrophs were preying upon the nanoflagellates. Mesozooplankton, however, appeared to exert the greatest impact on nanoflagellates. In the cascading experiments, the data suggest that mesozooplankton consume nanoflagellates, which resulted in a decrease in the predation impact of these organisms on the bacteria. This result is consistent with predator-prey cascades. The presence of the larger heterotrophs therefore, mediates the interactions between the primary bacterivores, the nanoflagellates, and the bacteria within the temporarily open/closed East Kleinemonde Estuary.
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Optimisation of a sampling protocol for long-term monitoring of temperate reef fishes
- Authors: Bennett, Rhett Hamilton
- Date: 2008
- Subjects: Marine parks and reserves -- South Africa , Coastal zone management -- South Africa , Fishery management -- South Africa , Reef fishes -- South Africa , Fish communities -- South Africa , Fish stock assessment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5309 , http://hdl.handle.net/10962/d1005154 , Marine parks and reserves -- South Africa , Coastal zone management -- South Africa , Fishery management -- South Africa , Reef fishes -- South Africa , Fish communities -- South Africa , Fish stock assessment
- Description: Marine Protected Areas (MPAs), the Ecosystem Approach to Fisheries management (EAF) and Integrated Coastal Management (ICM) have been identified as possible alternatives to traditional linefish management measures, which have largely failed. Monitoring and assessment of fish communities on a long-term basis is necessary, and will provide a means to evaluate the effectiveness of such management measures. Therefore, standardised protocols and optimal sampling methods for long-term monitoring (LTM) and assessment of coastal fish communities are essential. This study aimed to identify suitable methods and develop a protocol for assessment of inshore reef fish communities. A suitable location for evaluation of proposed methods was identified in the warm temperate biogeographical region of South Africa, encompassing the well-established Tsitsikamma Coastal National Park MPA and an adjacent exploited area. Chrysoblephus laticeps (roman) was identified as an indicator species for the study, as it has been well-studied and is well represented in the area. Underwater visual census (UVC) and controlled fishing were identified as suitable methods. UVC transects were found to be superior to point counts, in terms of sampling efficiency, variability, bias and required sample size. An effort of two angler hours per fishing station was shown to provide low catch variability, while at the same time a representative catch and low overall cost and required time. The methods were incorporated in a proposed sampling protocol, and evaluated. The methods were able to detect known differences between protected and exploited communities. It is recommended that LTM within protected areas, for detection of natural change, be focused on community-level indicators, while LTM in exploited areas, aimed at detection of anthropogenic change, be focused on species-level indicators. The proposed protocol with standardised methods will allow for comparisons across a network of LTM sites and provide the opportunity for a broad-scale assessment of the effects of environmental variables on reef fish stocks. The protocol developed in this study has application in other biogeographical regions in South Africa, and other parts of the world. Shift in the focus of much marine research, in South Africa and elsewhere, to LTM, highlights the relevance and timeous nature of this study.
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An investigation into the synergistic association between the major Clostridium cellulovorans cellulosomal endoglucanase and two hemicellulases on plant cell wall degradation
- Authors: Beukes, Natasha
- Date: 2008
- Subjects: Clostridium , Cellulose , Hemicellulose , Cellulase , Biomass conversion , Biomass energy -- South Africa , Energy crops -- South Africa , Bagasse -- Biodegradation , Pineapple -- Biodegradation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3968 , http://hdl.handle.net/10962/d1004027 , Clostridium , Cellulose , Hemicellulose , Cellulase , Biomass conversion , Biomass energy -- South Africa , Energy crops -- South Africa , Bagasse -- Biodegradation , Pineapple -- Biodegradation
- Description: The cellulosome is a multimeric enzyme complex that has the ability to metabolise a wide variety of carbonaceous compounds. Cellulosomal composition may vary according to the microbe’s nutritional requirement and allows for the anaerobic degradation of complex substrates. The complex substrates of interest in this research study were sugarcane bagasse and pineapple fibre waste, as they represent two important lignocellulosic, South African agricultural crops. The effective degradation of complex plant biomass wastes may present a valuable source of renewable compounds for the production of a variety of biofuels, for example bioethanol, and a variety of biocomposites of industrial importance. The identification of renewable energy sources for the production of biofuels is becoming increasingly important, as a result of the rapid depletion of the fossil fuels that are traditionally used as energy sources. An effective means of completely degrading lignocellulose biomass still remains elusive due to the complex heterogeneity of the substrate structure, and the fact that the effective degradation of the substrate requires a consortium of enzymes. The cellulosome not only provides a variety of enzymes with varying specificities, but also promote a close proximity between the catalytic components (enzymes). The close proximity between the enzymes promotes the synergistic degradation of complex plant biomass for the production of valuable energy products. Previous synergy studies have focused predominantly on the synergistic associations between cellulases; however, the synergy between hemicellulases has occasionally been documented. This research project established the synergistic associations between two Clostridium cellulovorans hemicellulases that may be incorporated into the cellulosome and a cellulosomal endoglucanase that is conserved in all cellulosomes. This research study indicated that there was indeed a synergistic degradation of the complex plant biomass (sugarcane bagasse and pineapple fibre). The degrees of synergy and the ratio of the enzymes varied between the two complex substrates. The initial degradation of the bagasse required the presence of all the enzymes and proceeded at an enhanced rate under sulphidogenic conditions; however, there was a low production of fermentable sugars. The low quantity of fermentable sugars produced by the degradation of the bagasse may be related to the chemical composition of the substrate. The sugarcane contains a high percentage of lignin forming a protective layer around the holocellulose, thus the glycosidic bonds are shielded extensively from enzymatic attack. In comparison, the initial degradation of the pineapple fibre required the action of hemicellulases, and proceeded at an enhanced rate under sulphidogenic conditions. The initial degradation of the pineapple fibre produced a substantially larger quantity of fermentable sugars in comparison to the bagasse. The higher production of fermentable sugars from the degradation of the pineapple fibre may be explained by the fact that this substrate may have a lower percentage of lignin than the bagasse, thus allowing a larger percentage of the glycosidic bonds to be exposed to enzymatic attack. The data obtained also indicated that the glycosidic bonds from the hemicellulosic components of the pineapple fibre shielded the glycosidic bonds of the cellulose component. The identification of the chemical components of the different substrates may allow for the initial development of an ideal enzyme complex (designer cellulosome) with enzymes in an ideal ratio with optimal synergy that will effectively degrade the complex plant biomass substrate.
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Synthesis, photochemical and photophysical properties of gallium and indium phthalocyanine derivatives
- Authors: Chauke, Vongani Portia
- Date: 2008
- Subjects: Phthalocyanines , Photochemotherapy , Electrochemistry , Gallium , Indium
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4375 , http://hdl.handle.net/10962/d1005040 , Phthalocyanines , Photochemotherapy , Electrochemistry , Gallium , Indium
- Description: The syntheses of octasubstituted and unsusbstitituted Gallium(III) chloride and indium(III) chloride phthalocyanines (GaPc and InPc), their photophysical, photochemical and nonlinear optical parameters are hereby presented. The photocatalytic oxidation of 1-hexene using the synthesized GaPc and InPc complexes as well as electrochemical characterization is also presented in this thesis. Fluorescence quantum yields do not vary much among the four Ga complexes, except for complex 21c; therefore it was concluded that the effect of substituents is not significant among them. Solvents however, had an effect on the results. Lower Φ[subscript F] values were obtained in low viscosity solvents like toluene, relative to highly viscous solvents, such as DMSO. The triplet quantum yields were found to be lower in DMSO than in DMF and toluene. The rate constants for fluorescence, intersystem crossing and internal conversion as well as fluorescence and triplet lifetimes are reported. Photodegradation and singlet oxygen quantum yields have also been reported. There was no clear correlation between the latter parameters. It was however established that the four gallium MPcs were stable, within the allowed stability range for phthalocyanines. High quantum yields of triplet state (Φ[subscript T] ranging from 0.70 to 0.91 in dimethysulfoxide, DMSO) and singlet oxygen generation (Φ[subscript greek capital letter delta], ranging from 0.61 to 0.79 in DMSO) were obtained. Short triplet lifetimes 50 to 60 μs were obtained in DMSO). Calculated non-linear parameters of these complexes are compared with those of the corresponding GaPc derivatives and tetrasubstituted GaPc and InPc complexes. The optical limiting threshold intensity (I[subscript lim]) values for the InPc and GaPc derivatives were calculated and compared with those of corresponding tetrasubstituted InPc and GaPc complexes. The octasubstituted were found to be better optical limiters. Photocatalytic oxidation of 1-hexene by GaPc (21a-c) and InPc (22a-c) derivatives is also presented. The photocatalytic oxidation products for 1-hexene were 1,2- epoxyhexane and 1-hexen-3-ol. The % conversion values of 1-hexene and % selectivity of 1,2-epoxyhexane were generally higher for InPc derivatives. Even though InPc derivatives showed better photocatalytic results than GaPc derivatives, the former were less stable relative to the latter. Both type I and type II mechanism were implicated in the photocatalysis mechanism.
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The variability and predictability of the IRI shape parameters over Grahamstown, South Africa
- Authors: Chimidza, Oyapo
- Date: 2008
- Subjects: Ionosphere -- Mathematical models -- South Africa , Atmosphere, Upper -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5496 , http://hdl.handle.net/10962/d1005282 , Ionosphere -- Mathematical models -- South Africa , Atmosphere, Upper -- South Africa
- Description: The International Reference Ionosphere (IRI) shape parameters B0, B1, and D1 provide a representation of the shape of the F2 layer, the thickness of the F2 layer and the shape of the F1 layer of the ionosphere respectively. The aim of this study was to examine the variability of these parameters using Grahamstown, South Africa (33.3±S, 26.5±E) ionosonde data and determine their predictability by the IRI-2001 model. A further aim of this study was to investigate developing an alternative model for predicting these parameters. These parameters can be determined from electron density profiles that are inverted from ionograms recorded with an ionosonde. Data representing the B0, B1 and D1 parameters, with half hourly or hourly intervals, were scaled and deduced from the digital pulse sounder (DPS) ionosonde for the period April 1996 to December 2006. An analysis of the diurnal, seasonal, and solar variations of the behaviour of these parameters was undertaken for the years 2000, 2004 and 2005 using monthly medians. Comparisons between the observational results and that of the IRI model (IRI 2001 version) indicate that the IRI-2001 model does not accurately represent the diurnal and seasonal variation of the parameters. A preliminary model was thus developed using the technique of Neural Networks (NNs). All available data from the Grahamstown ionosonde from 1996 to 2006 were used in the training of the NNs and the prediction of the variation of the shape parameters. Inputs to the model were the day number, the hour of day, the solar activity and the magnetic index. Comparisons between the preliminary NN model and the IRI-2001 model indicated that the preliminary model was more accurate at the prediction of the parameters than the IRI-2001 model. This analysis showed the need to improve the existing IRI model or develop a new model for the South African region. This thesis describes the results from this feasibility study which show the variability and predictability of the IRI shape parameters.
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Toward the development of a rearing protocol for juvenile dusky kob, Argyrosomus japonicus (Pisces: Sciaenidae)
- Authors: Collett, Paul David
- Date: 2008
- Subjects: Argyrosomus -- South Africa , Mariculture -- South Africa , Fishes -- Nutrition -- South Africa , Fishes -- Growth -- South Africa , Fish trade -- South Africa , Sciaenidae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5325 , http://hdl.handle.net/10962/d1005171 , Argyrosomus -- South Africa , Mariculture -- South Africa , Fishes -- Nutrition -- South Africa , Fishes -- Growth -- South Africa , Fish trade -- South Africa , Sciaenidae
- Description: The South African mariculture industry is developing the rearing technology of indigenous fish species. Dusky kob (Argyrosomus japonicus) has high-quality flesh and a good market demand. Research is needed to determine the environmental requirements under which growth of dusky kob is optimised. This study assessed the effect of temperature, light intensity, feeding frequency and stocking density, respectively, on growth, feed conversion ratio (FCR) and survival of juvenile dusky kob within a weight range of 10-60 g fish⁻¹) in a series of four growth trials. The effect of temperature on growth and FCR was assessed at 14 temperatures from 17 to 28°C. The temperature for best growth was estimated to be 25.3 °C, while 21.4 °C was the temperature at which the best FCR was achieved. A growth trial testing the effect of light intensity on growth showed that light intensity did not affect growth in the range of 23 – 315 lx. Fish fed a restricted ration of 3.6% body weight per day (chapter 2) or 3.41% body weight per day (chapter 4) had a better FCR than fish fed to satiation. A trial to assess the effect of feeding frequency on growth and FCR showed that fish fed both two or three times daily grew better than those fed once daily. FCR was best in fish that were fed once or twice daily. Preliminary analysis of the results from a stocking density trial showed that stocking density in the range of 10 – 50 kg m⁻³ did not affect growth of juvenile dusky kob. It is recommended to culture dusky kob at a temperature of 25.3 - 21.4 °C at stocking densities up 50 kg m⁻³ to maximise growth. However, a study is needed to determine the environmental conditions needed to maximise profit under commercial conditions. The results allow the South African industry the opportunity to assess the culture potential of this species. In addition, the results will help develop protocols that can be used in other South African candidate aquaculture species such as silver kob, yellowtail, white-stumpnose and sole.
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Temperature dependence of the HartRAO pointing model
- Authors: Copley, Charles Judd
- Date: 2008
- Subjects: Astronomical instruments , Observatories -- South Africa , Telescopes , Astronomical observatories , Astronomy -- Data processing , Antennas (Electronics)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5491 , http://hdl.handle.net/10962/d1005277 , Astronomical instruments , Observatories -- South Africa , Telescopes , Astronomical observatories , Astronomy -- Data processing , Antennas (Electronics)
- Description: This thesis investigates control aspects of the Hartebeeshoek Radio Astronomy Observatory (HartRAO) antenna. The installation of a new 22 GHz receiver has required the pointing accuracy to be improved to less than 4 mdeg. The effect of thermal conditions on the the HartRAO antenna pointing offset is investigated using a variety of modelling techniques including simple geometric modelling, neural networks and Principal Component Analysis (PCA). Convincing results were obtained for the Declination pointing offset, where applying certain model predictions to observations resulted in an improvement in Declination pointing offset from 5.5 mdeg to 3.2 mdeg (≈50%). The Right Ascension pointing model was considerably less convincing with an improvement of approximately from 5.5 mdeg to 4.5 mdeg (≈20%) in the Right Ascension pointing offset. The Declination pointing offset can be modelled sufficiently well to reduce the pointing offset to less than 4 mdeg, however further investigation of the underlying causes is required for the Right Ascension pointing offset.
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Lattice-valued uniform convergence spaces the case of enriched lattices
- Authors: Craig, Andrew Philip Knott
- Date: 2008
- Subjects: Lattice theory , Uniform spaces , Convergence
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5411 , http://hdl.handle.net/10962/d1005225 , Lattice theory , Uniform spaces , Convergence
- Description: Using a pseudo-bisymmetric enriched cl-premonoid as the underlying lattice, we examine different categories of lattice-valued spaces. Lattice-valued topological spaces, uniform spaces and limit spaces are described, and we produce a new definition of stratified lattice-valued uniform convergence spaces in this generalised lattice context. We show that the category of stratified L-uniform convergence spaces is topological, and that the forgetful functor preserves initial constructions for the underlying stratified L-limit space. For the case of L a complete Heyting algebra, it is shown that the category of stratified L-uniform convergence spaces is cartesian closed.
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The status and prognosis of the smoothhound shark (mustelus mustelus) fishery in the Southeastern and Southwestern Cape coasts, South Africa
- Authors: Da Silva, Charlene
- Date: 2008
- Subjects: Osteichthyes -- South Africa Mustelus -- South Africa Sharks -- South Africa Fishery management -- South Africa Fisheries -- South Africa Chondrichthyes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5200 , http://hdl.handle.net/10962/d1003287
- Description: Global trends in commercially valuable teleost fisheries point to substantial deterioration in population size, offering limited potential for increased harvests. Consequently a shift in focus towards alternative fisheries has increased fishing effort towards targeting chondrichthyans as a possible solution to meet global demands. The life-history traits of chondrichthyans make them poor candidates for resolving economic and nutritional security as these make them particularly vulnerable to anthromorphic influences. Current fisheries management approaches based on centralized government intervention have proved inadequate. This failure of current management approaches is often linked with poor co-operation by industry with government when collecting fishery-dependent data. As management decisions are based on quantitative estimates from fishery assessment modes data collected are often of poor quality. Co-management with its implied power-sharing arrangement between government and fishing communities has been proposed as a more realistic alternative. The motivation within industry to collect high quality data can only be created with a feeling of ownership. The decline in linefish species in South Africa has led to increased exploitation of demersal sharks such as Mustelus mustelus. Their status as one of the target and by-catch species of South Africa's shark fisheries necessitated resource assessment. Age, growth, maturity and mortality calculations for M mustelus were made from data collected from 1983-2006. The maximum observed age for M mustelus was 25 years. Estimated von Bertalanffy growth parameters from observed length-at-age for combined sexes, females and males were L∞ = 1946.16 mm TL, K = 0.08 year⁻' , to = -3.63 year⁻'; L∞ = 2202.21mm, K = 0.05 year⁻', to = 4.67 years; and L∞ = 1713.19 mm TL, K = 0.08 year⁻' and to = -4.36 years, respectively. Instantaneous total mortality (Z) was estimated at 0.16 y⁻', whilst natural mortality (M) for M mustelus was estimated at 0.05 y⁻'. The age and length at 50% maturity was determined for combined sexes, females and males at 1216 mm TL corresponding to an age of 9.93 years, 1234 mm (TL) and 10.75 years, and 1106 mm TL and 9.1 years respectively. The smoothhound shark resource off the south-eastern and south-western Cape coast was assessed by three dynamic pool models; yield per recruit, spawner biomass per recruit and an extended yield and spawner biomass per recruit. Due to the longevity of elasmobranches the per-recruit model was extended over a 20 year time-frame to simulate resource responses to management options. Fo.1 was estimated as 0.034 year⁻' and F MAX was estimated as 0.045 year⁻'. FsB50 was estimated as 0.031. The extended per-recruit model tested the outcome of different management scenarios, Size and effort control showed the least probability of pristine biomass falling below 20% of current levels in 20 years (where selectivity was set at 3 years). The replacement yield model showed that the average catches over the past decade are 2.5 times higher than the replacement yield is on the South Coast and 1.30 on the West Coast. A more realistic level optimising yield would be at 0.05 with a selection pattern at 3 years, where the probability of the biomass falling to below 20% of pristine pre-exploited levels in twenty years becomes negligible. A comparison of the models showed that current catches need to be halved for exploitation of smoothhound sharks to be sustainable. The results of this study indicate a need for a management intervention for M mustelus in South Africa with regards to potential overexploitation and collection of quality data for further assessments. A demersal identification key was developed as part of this study, which should aid monitoring officials in accurately identifying shark logs for collecting data. This study showed how adopting a management plan with the inclusion of comanagement concepts would improve the quality of data collected and increase monitoring of fishing activities. The inclusion of co-management is possible due to the unique bottle neck created by few demersal shark processing facilities actively exporting shark. A fishery management plan was compiled proposing several management options including size and effort controls.
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Network-layer reservation TDM for ad-hoc 802.11 networks
- Authors: Duff, Kevin Craig
- Date: 2008
- Subjects: Computer networks -- Access control , Computers -- Access control , Computer networks -- Management , Time division multiple access , Ad hoc networks (Computer networks)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4574 , http://hdl.handle.net/10962/d1002773 , Computer networks -- Access control , Computers -- Access control , Computer networks -- Management , Time division multiple access , Ad hoc networks (Computer networks)
- Description: Ad-Hoc mesh networks offer great promise. Low-cost ad-hoc mesh networks can be built using popular IEEE 802.11 equipment, but such networks are unable to guarantee each node a fair share of bandwidth. Furthermore, hidden node problems cause collisions which can cripple the throughput of a network. This research proposes a novel mechanism which is able to overcome hidden node problems and provide fair bandwidth sharing among nodes on ad-hoc 802.11 networks, and can be implemented on existing network devices. The scheme uses TDM (time division multiplexing) with slot reservation. A distributed beacon packet latency measurement mechanism is used to achieve node synchronisation. The distributed nature of the mechanism makes it applicable to ad-hoc 802.11 networks, which can either grow or fragment dynamically.
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Risk assessment and the effects of overhead work - an automotive industry example
- Authors: Elliott, Andrew Brent
- Date: 2008
- Subjects: Human engineering -- South Africa , Industrial safety -- South Africa , Automobile industry workers -- South Africa -- Health risk assessment , Automobile industry and trade -- South Africa -- Safety measures , Musculoskeletal system -- Wounds and injuries -- Prevention , Lifting and carrying -- Safety measures , Work measurement , Posture
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5132 , http://hdl.handle.net/10962/d1005211 , Human engineering -- South Africa , Industrial safety -- South Africa , Automobile industry workers -- South Africa -- Health risk assessment , Automobile industry and trade -- South Africa -- Safety measures , Musculoskeletal system -- Wounds and injuries -- Prevention , Lifting and carrying -- Safety measures , Work measurement , Posture
- Description: The focus of this investigation was an analysis of the work demands being placed on South African automotive industry workers as there is a recognised problem with regard to the prevalence of musculoskeletal disorders (MSDs). Preliminary work was conducted to highlight the dominant risks and areas which elicited higher numbers of MSDs within the chosen automotive plant. An area of concern was highlighted through medical record analysis and the use of risk assessment tools, thereby prioritising the need for ergonomic intervention. In particular, the effects of varying restricted and overhead work heights on the biomechanical, physiological and psychophysical responses of an individual were investigated. Twenty-eight subjects were required to complete sixteen conditions. The conditions consisted of the adoption of restricted and upright overhead static postures, with half requiring the holding of four kilograms of weight in the hands and the remaining eight conditions having no weight. Testing was carried out using an electromyography unit, ergospirometer and a perceptual Body Discomfort Map and Scale. This involved a habituation and testing session. The results of the testing revealed the biomechanical and physiological responses were dependant on the change in height. Body discomfort was also shown to be variable over the changing height conditions. This indicates that there is a significant effect of height on an individual’s responses during overhead work. The extreme restricted (-200mm and -100mm) and upright (+300mm and +400mm) overhead conditions within this study were limiting, as they elicited the highest muscle activation, physiological responses and body discomfort ratings. Positions that are preferable to adopt, which were identified from the results in this study, indicate conditions closer to head height (0mm and +100mm) were favourable. The results therefore illustrate how awkward working postures during work are likely to elicit higher demands from an individual, which could lead to an increased risk for the development of a musculoskeletal disorder. The added factor of weight elicited significant results over all variables, excluding a respiratory The focus of this investigation was an analysis of the work demands being placed on South African automotive industry workers as there is a recognised problem with regard to the prevalence of musculoskeletal disorders (MSDs). Preliminary work was conducted to highlight the dominant risks and areas which elicited higher numbers of MSDs within the chosen automotive plant. An area of concern was highlighted through medical record analysis and the use of risk assessment tools, thereby prioritising the need for ergonomic intervention. In particular, the effects of varying restricted and overhead work heights on the biomechanical, physiological and psychophysical responses of an individual were investigated. Twenty-eight subjects were required to complete sixteen conditions. The conditions consisted of the adoption of restricted and upright overhead static postures, with half requiring the holding of four kilograms of weight in the hands and the remaining eight conditions having no weight. Testing was carried out using an electromyography unit, ergospirometer and a perceptual Body Discomfort Map and Scale. This involved a habituation and testing session. The results of the testing revealed the biomechanical and physiological responses were dependant on the change in height. Body discomfort was also shown to be variable over the changing height conditions. This indicates that there is a significant effect of height on an individual’s responses during overhead work. The extreme restricted (-200mm and -100mm) and upright (+300mm and +400mm) overhead conditions within this study were limiting, as they elicited the highest muscle activation, physiological responses and body discomfort ratings. Positions that are preferable to adopt, which were identified from the results in this study, indicate conditions closer to head height (0mm and +100mm) were favourable. The results therefore illustrate how awkward working postures during work are likely to elicit higher demands from an individual, which could lead to an increased risk for the development of a musculoskeletal disorder. The added factor of weight elicited significant results over all variables, excluding a respiratory individual.
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Cranial ontogeny of the sole, dagetichthys marginatus (soleidae), with considerations on the feeding ability of larvae and early juveniles
- Authors: Ende, Stephan Siegfried Werner
- Date: 2008
- Subjects: Soleidae , Fishes -- Larvae -- Food , Flatfishes -- Nutrition , Artemia
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5250 , http://hdl.handle.net/10962/d1005093 , Soleidae , Fishes -- Larvae -- Food , Flatfishes -- Nutrition , Artemia
- Description: The overall aim of this study was to gain a better understanding of the feeding mechanisms of the larvae and early juveniles of the sole, Dagetichthys marginatus (Soleidae), with which to assess the suitability of current feeding protocols and to facilitate the development of an appropriate pelleted feed. This was achieved by examining the ontogeny of the cranium of laboratory reared sole, with particular emphasis on those elements associated with feeding and by comparing the cranium of juvenile fish with that of adult fish. At 4 dah (days after hatch) Dagetichthys marginatus larvae develop the first rudimentary branchial arches that facilitates the capture and ingestion of food items. Subsequent development of cranial structures, such as the oral jaws, suspensorium, neurocranium, hyoid and branchial arches and the opercular apparatus enables the larvae, at 16 dah, to switch from ram feeding to suction feeding on live prey. The use of live Artemia nauplii from 4 to 16 dah is therefore appropriate. The first morphological asymmetries developed at 16 dah in the dentaries and at 22 dah the maxillae and the premaxillae began to show asymmetries. Teeth were present only on the blind side of the oral jaw elements and during this period (16 to 22 dah) the existing elements began to ossify. From 16 to 35 dah the standard feeding protocol consists of a combination of pelagic (Artemia metanauplii) and benthic prey (dead, frozen Artemia nauplii) and from 25 dah onwards a sinking pellet is provided. The time (dah) at which frozen Artemia and sinking pellets were provided, appropriately corresponded to the initiation of benthic feeding behaviour. However at this stage the use of pelagic Artemia metanauplii is inappropriate and unnecessary. At 31 dah the cranial morphology resembled that of adult fish. Adult D. marginatus display extreme asymmetries among the elements of the oral jaws, the suspensorium and certain elements of the neurocranium. Elements on the blind side are larger and more robust than those on the ocular side and are adapted for feeding, while those on the ocular side appear to have a respiratory function. From 31 dah the cranial elements are identical to those of adult fish, suggesting that no further feeding behavioural changes occurred and that a sinking pellet, of which the nutrient composition meets the requirements of the fish, would be appropriate for ongrowing.
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Drought responses of C3 and C4 (NADP-ME) Panicoid grasses
- Authors: Frole, Kristen Marie
- Date: 2008
- Subjects: Botany -- Research , Grasses -- Physiology -- South Africa , Grasses -- Effect of drought on , Grasses -- Drought tolerance , Plant-water relationships
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4193 , http://hdl.handle.net/10962/d1003762 , Botany -- Research , Grasses -- Physiology -- South Africa , Grasses -- Effect of drought on , Grasses -- Drought tolerance , Plant-water relationships
- Description: The success of C₄ plants lies in their ability to concentrate CO₂ at the site of Rubisco thereby conferring greater efficiencies of light, water and nitrogen. Such characteristics should advantage C₄ plants in arid, hot environments. However, not all C₄ subtypes are drought tolerant. The relative abundance of NADP-ME species declines with increasing aridity. Furthermore, selected species have been demonstrated as being susceptible to severe drought showing metabolic limitations of photosynthesis. However there is a lack of phylogenetic control with many of these studies. The aims of this study were to determine whether the NADP-ME subtype was inherently susceptible to drought by comparing six closely related C₃ and C₄ (NADP-ME) Panicoid grasses. Gas exchange measurements were made during a natural rainless period and a controlled drought / rewatering event. Prior to water stress, the C₄ species had higher assimilation rates (A), and water use efficiencies (WUE[subscript leaf]) than the C₃ species, while transpiration rates (E) and stomatal conductances (g[subscript s]) were similar. At low soil water content, the C₃ species reduced gs by a greater extent than the C₄ species, which maintained higher E during the driest periods. The C₄ species showed proportionally greater reductions in A than the C₃ species and hence lost their WUE[subscript leaf] and photosynthetic advantage. CO₂ response curves showed that metabolic limitation was responsible for a greater decrease in A in the C₄ type than the C₃ type during progressive drought. Upon re-watering, photosynthetic recovery was quicker in the C species than the C₄ species. Results from whole plant measurements showed that the C₄ type had a significant whole plant water use efficiency advantage over the C₃ type under well-watered conditions that was lost during severe drought due to a greater loss of leaf area through leaf mortality rather than reductions in plant level transpiration rates. The C₃ type had xylem characteristics that enhanced water-conducting efficiency, but made them vulnerable to drought. This is in contrast to the safer xylem qualities of the C₄ type, which permitted the endurance of more negative leaf water potentials than the C₃ type during low soil water content. Thus, the vulnerability of photosynthesis to severe drought in NADP-ME species potentially explains why NADP-ME species abundance around the world decreases with decreasing rainfall.
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Agathis bishopi (Nixon) (Hymenoptera: braconidae) its biology and usefulness as a biological control agent for false codling moth (FCM), Thaumatotibia leucotreta (Meyrick) (Lepidoptera: tortricidae), on citrus bishopi (Nixon) (Hymenoptera: braconidae) its biology and usefulness as a biological control agent for false codling moth (FCM), Thaumatotibia leucotreta (Meyrick) (Lepidoptera: tortricidae), on citrus
- Authors: Gendall, Kierryn Leigh
- Date: 2008
- Subjects: Cryptophlebia leucotreta -- Parasites , Cryptophlebia leucotreta -- Control , Pests -- Biological control , Tortricidae , Braconidae , Citrus -- Diseases and pests
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5635 , http://hdl.handle.net/10962/d1005317 , Cryptophlebia leucotreta -- Parasites , Cryptophlebia leucotreta -- Control , Pests -- Biological control , Tortricidae , Braconidae , Citrus -- Diseases and pests
- Description: The false codling moth, Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae), is one of the major pests of citrus in South Africa, the others being mealybug, Mediterranean fruit fly, bollworm and some mites. Due to problems such as the expense of pesticides, insects evolving pesticide resistance (Hogsette 1999), chemical residue on the skin of export fruit and the negative impact of pesticides on the environment, it became necessary to find alternative methods for pest control (Viggiani 2000). Agathis bishopi (Nixon) (Hymenoptera: Braconidae), a larval parasitoid of false codling moth known only from the Sundays River Valley area (Sishuba 2003), offers a means of control for the pest. A total of 11 389 navel oranges were collected from various orchards in the Addo/Kirkwood area, and false codling moth larvae infested 36.09% of the fruit. A single parasitoid species, A. bishopi, was reared from these larvae. In 2006 the highest parasitism rate, 11.43%, was recorded in May and in 2007, the highest parasitism rate, 13.27%, was in April. Agathis bishopi parasitizes larvae in instars 2 and 3, possibly due to the accessibility of these younger instars to the female parasitoid and possibly due to the length of the life cycle of this koinobiont. Second instar hosts yielded the highest number of parasitoids, and there was no emergence of parasitoids from fifth instar larvae. Females of A. bishopi live for 18.5 days (n = 20; S.E. = 3.1) and males for 8.25 days (n = 20; S.E. = 1.23). Females produce an average of 23 offspring in a lifetime, while female false codling moths produce about 800 eggs each. A high number of parasitoids will be required per hectare to reduce the population of false codling moth. Captive rearing of A. bishopi proved difficult due to viral and fungal contamination. Agathis bishopi has potential for use in an integrated pest management programme once the hurdle of mass-rearing has been overcome.
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Isolation, purification and characterization of a 'factor' from Fusarium oxysporum responsible for platinum nanoparticle formation
- Authors: Govender, Yageshni
- Date: 2008
- Subjects: Nanoparticles , Platinum , Fusarium oxysporum , Fungi , Hydragenase
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3923 , http://hdl.handle.net/10962/d1003982 , Nanoparticles , Platinum , Fusarium oxysporum , Fungi , Hydragenase
- Description: Nanoparticles are microscopic particles in the nanometre range of between 1-100 nm. A wide variety of metal nanoparticles have been found to be produced by prokaryotic and eukaryotic organisms including several fungal species, when exposed to solutions containing metal salts. Previous studies have suggested that this bioreduction of metal particles may occur via an active reductase/hydrogenase enzyme process where H2 is the electron donor and positively charged platinum species act as the electron acceptors becoming reduced to a neutral metal nanoparticle. In view of this on going research, the current study investigated the “factors” in the fungus Fusarium oxysporum which were responsible for platinum nanoparticle formation. The fungus F.oxysporum was used in this study as it has been previously shown to produce a variety of nanoparticles including gold and silver. During exposure of the biomass to H2PtCl6 the initial response to the platinum salts was metal internalisation and subsequent reduction of H2PtCI6 to produce platinum nanoparticles. The observed localization and distribution of platinum precipitates provided some evidence for a hydrogenase mediated bioreduction of platinum salts to produce nanoparticles. Factors secreted by the fungus into the extracellular fluids, were shown to be responsible for platinum nanoparticle formation. From the identification, purification and characterisation studies it was concluded that a hydrogenase and other “factors” were responsible for platinum nanoparticle formation in F.oxysporum. Purification of the hydrogenase by freeze-drying and Sephacryl S200 size exclusion- ion exchange chromatography revealed the enzyme to be a dimer with a 29.4 and 44.5 kDa when analysed by a 10 % SDS-PAGE. Characterisation of the enzyme revealed optimal activity at a pH of 7.5 and temperature of 38 °C while it exhibited a poor thermal stability with a half life of 36 minutes. The kinetic parameters Vmax and Km were 3.16 U ml-1 and 3.64 mM respectively. The purified hydrogenase was used in subsequent experiments for the reduction of platinum salts, H2PtCl6 and PtCl2. the results indicated an over 90 % reduction of the platinum salts and TEM micrographs indicated the production of platinum nanoparticles under the various experimental conditions.
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An assessment of the companion modelling approach in a context of negotiating water allocation strategies : the case of the Kat River Valley, Eastern Cape, South Africa
- Authors: Gumede, Felicity Hlengiwe
- Date: 2008
- Subjects: Water supply -- South Africa -- Kat River Valley -- Management , Water resources development -- South Africa -- Kat River Valley , Integrated water development -- South Africa -- Kat River Valley
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4820 , http://hdl.handle.net/10962/d1005494 , Water supply -- South Africa -- Kat River Valley -- Management , Water resources development -- South Africa -- Kat River Valley , Integrated water development -- South Africa -- Kat River Valley
- Description: This Masters research took place in the Kat River Valley in the Eastern Cape, South Africa. The Kat River Valley is a semi-rural catchment that covers an area of approximately 1700km² and is characterized by a complicated history of dispossession and resettlement. Farming is the main activity that is practiced in the area. This includes the farming of citrus at a commercial scale, rangeland stock farming and small-scale vegetable farming. The economy of the catchment is enhanced mostly by commercial citrus farming, which consumes by far the largest amount of water in the river through irrigation. Water allocation is a burning issue among water users in the catchment and needs to be negotiated taking into consideration social, economic and environment impacts. The aim of this study is to describe, discuss and evaluate the Companion Modelling (ComMod) approach, which used a simulation model and a role-playing game related to the model in order to facilitate and develop negotiating skills as well as build capacity in decision-making amongst local stakeholders for water resource management in the Kat River Valley. The ComMod approach, developed by a group of Companion Modellers, is a community-based science approach that emerged in the 1990s. The ComMod approach is used in order to facilitate collective learning, negotiation and institutional innovation in dealing with resource management complexities faced by rural communities. Through ComMod, the model (KatAWARE) and its related role-playing game was developed by having the contact with local stakeholders. The information to feed the model and the role-playing game came from informal interviews, surveys, geographic information systems (GIS), workshops and focus groups. The use of workshops in the implementation of ComMod was a success. Results show that (1) new knowledge was acquired, which allowed stakeholders to have a broad understanding of a catchment system. (2) Awareness was created about complex systems and enabled stakeholders to see an individual action into to the broader system. (3) Strong interrelationships were fostered amongst different water users, which allowed stakeholders to share their view points. The ComMod process was however associated with a number of limitations, many of which resulted from the constraints that were imposed by the socio-economic background of the study area. Nevertheless, the outcome of the study shows that the ComMod process was useful in helping the Kat River Water Users Association (KRWUA) stakeholders develop negotiating skills regarding water allocation strategies for the development of the Catchment Management Plan.
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The development of a putative microbial product for use in crop production
- Authors: Gumede, Halalisani
- Date: 2008
- Subjects: Agricultural productivity , Agriculture -- Economic aspects , Microbial products , Bacterial diseases of plants , Biological pest control agents , Lettuce -- Diseases and pests , Crops -- Nutrition , Bacillus (Bacteria) , Phytopathogenic microorganisms -- Control
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3945 , http://hdl.handle.net/10962/d1004004 , Agricultural productivity , Agriculture -- Economic aspects , Microbial products , Bacterial diseases of plants , Biological pest control agents , Lettuce -- Diseases and pests , Crops -- Nutrition , Bacillus (Bacteria) , Phytopathogenic microorganisms -- Control
- Description: The challenges faced by the agricultural sector especially around improving production yields using environmentally friendly solutions have received market attention. Biological intervention can range from application of biological products to enhance the nutritional value of crops or to control plant pathogens. Biostart, a biological product that demonstrated growth enhancement when applied in lettuce crops is currently in the market. The product is comprised of a consortium of bacterial isolates (Bacillus licheniformis, Brevibacillus laterosporus and Bacillus laterosporus) but the contribution of the individual isolates to growth enhancement had not been elucidated. Green house experiments on lettuce seedlings with individual and mixed treatments were commissioned to determine such contribution. There was either no or marginal growth enhancement observed in the experiments. The results showed that the product was effective as a consortium and not as individual isolates. Further isolation and screening for potential Bacilli with antifungal properties was undertaken. An isolate identified as Bacillus subtilis that demonstrated inhibition against a wide spectrum of fungi, and especially the phytopathogenic Verticillium dahliae and Fusarium oxysporum, was successfully identified. The isolate was cryo-preserved and cultivated to significant levels at bench scale. A characterized comparison of different putative products with known systematic fungicide showed potential application even of heat treated products. The product showed control V. dahliae when tested in green houses with potatoes and tomatoes as test crops. This isolate has been targeted for further development as a biological control product.
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A feasibility study into total electron content prediction using neural networks
- Authors: Habarulema, John Bosco
- Date: 2008
- Subjects: Electrons , Neural networks (Computer science) , Global Positioning System , Ionosphere , Ionospheric electron density
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5466 , http://hdl.handle.net/10962/d1005251 , Electrons , Neural networks (Computer science) , Global Positioning System , Ionosphere , Ionospheric electron density
- Description: Global Positioning System (GPS) networks provide an opportunity to study the dynamics and continuous changes in the ionosphere by supplementing ionospheric measurements which are usually obtained by various techniques such as ionosondes, incoherent scatter radars and satellites. Total electron content (TEC) is one of the physical quantities that can be derived from GPS data, and provides an indication of ionospheric variability. This thesis presents a feasibility study for the development of a Neural Network (NN) based model for the prediction of South African GPS derived TEC. The South African GPS receiver network is operated and maintained by the Chief Directorate Surveys and Mapping (CDSM) in Cape Town, South Africa. Three South African locations were identified and used in the development of an input space and NN architecture for the model. The input space includes the day number (seasonal variation), hour (diurnal variation), sunspot number (measure of the solar activity), and magnetic index(measure of the magnetic activity). An attempt to study the effects of solar wind on TEC variability was carried out using the Advanced Composition Explorer (ACE) data and it is recommended that more study be done using low altitude satellite data. An analysis was done by comparing predicted NN TEC with TEC values from the IRI2001 version of the International Reference Ionosphere (IRI), validating GPS TEC with ionosonde TEC (ITEC) and assessing the performance of the NN model during equinoxes and solstices. Results show that NNs predict GPS TEC more accurately than the IRI at South African GPS locations, but that more good quality GPS data is required before a truly representative empirical GPS TEC model can be released.
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