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Studies on the neuro-physiology of a free-living Platyhelminth

  • Authors: Boyle, Sheila A
  • Date: 1961
  • Subjects: Platyhelminthes
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5902 , http://hdl.handle.net/10962/d1013541
  • Description: From Resumé: The general picture of the physiology of invertebrate neuro-muscular systems as contrasted with the classical vertebrate system is presented and it is clear that one of the groups least investigated is the Phylum Platyhelminthes. An examination of the properties of the myo-neural system of a platyhelminth should be of interest, not only in itself, but also because of a possible relationship with the coelenterates, whose neuro-muscular system has been fairly extensively investigated. The aim of the present work was to determine some of the properties of the myo-neural system of a platyhelminth, and more especially those which would make possible a comparison with the myo-neural systems of other invertebrate phyla, and most particularly with the coelenterates. A preparation consisting of an entire decerebrate animal was used. The general anatomy of the musculature and basic plan of the nervous system are presented to facilitate an understanding of the experiments described, but a detailed examination of the relationships of nerves and muscles or of the innervation of the latter was not attempted. The spontaneous activity of such preparations was recorded kymographically and the characteristics of this activity under 'normal' conditions are described, together with observations on the effects on the normal spontaneous activity of treatments with sea waters of different ionic composition and with a number of drugs. The responses of the preparation to controlled electrical stimulation are also reported. The results are analysed and compared with the results of similar investigations on other invertebrate preparations, in particular the Anthozoa, and an interpretation of these results in terms of platyhelminth-coelenterate relations is discussed.
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Investigating the role of Hsp90 and LRP1 in FN matrix dynamics

  • Authors: Boël, Natasha Marie-Eraine
  • Date: 2016
  • Subjects: Extracellular matrix , Molecular chaperones , Heat shock proteins , Cancer , Fibronectins
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10962/2713 , vital:20319
  • Description: Fibronectin (FN), a matrix protein responsible for regulating processes including migration and differentiation, is secreted as a soluble dimer which is assembled into an insoluble extracellular matrix. The dynamics of FN matrix assembly and degradation play a large role in cell migration and invasion contributing to the metastatic potential of cancer cells. Previous studies from our group have shown the direct binding of Hsp90 and FN in vitro and that inhibition of Hsp90 with novobiocin (NOV) caused internalisation of the FN matrix. However, the receptor mediating this internalisation is currently unknown. Low density lipoprotein 1 (LRP1) is a likely candidate as it is a ubiquitous receptor responsible for regulating internalisation of diverse ligands and is known to bind both Hsp90 and FN. We used wild type and knockout LRP1 cell lines to study the endocytosis of FN via this receptor. Here, we demonstrate that LRP1-deficient cells accumulated greatly increased levels of FN and were found to be less sensitive to pharmacological inhibition of Hsp90 by NOV. LRP1-expressing MEF-1 and Hs578T breast cancer cells experienced an increase in total FN in response to NOV, at concentrations below the EC50 value, followed by a dose-dependent loss of FN. We attributed greater FN levels to a loss of extracellular FN matrix coupled with increased internalisation of FN. Cell-surface biotinylation and DOC assays showed that loss of extracellular FN was specific to LRP1-expressing MEF-1 cells. Furthermore, we demonstrate that the loss of extracellular FN is not affected by changes in FN mRNA levels as determined by qRT-PCR, and that treatment with NOV resulted in the accelerated degradation of FN in the presence of cycloheximide. Immunoprecipitation studies reveal a putative complex exists between FN, Hsp90 and LRP1 in both cancer and non-cancer cells which is not perturbed by NOV. Western analyses revealed increased proteolytic processing of LRP1 in response to NOV which we proposed, based on literature, to modulate signalling pathways as a potential mechanism for regulating FN turnover. Moreover, using wound healing assays we identified increased migration to be one of the consequences associated with loss of extracellular FN by Hsp90 inhibition but only in cells containing LRP1. In summary, this study provides new insights into the Hsp90-LRP1 mediated loss of FN matrix and also reveals for the first time the functional consequence related to FN turnover by NOV was an increase in migration in LRP1-expressing cells.
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The comparative bioavailability and in vitro assessment of solid oral dosage forms of paracetamol

  • Authors: Braae, Karen
  • Date: 1981 , 2013-04-02
  • Subjects: Acetaminophen , Bioavailability , Drugs -- Bioavailability , Drugs -- Dosage forms , Analysis of variance
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:3827 , http://hdl.handle.net/10962/d1006288 , Acetaminophen , Bioavailability , Drugs -- Bioavailability , Drugs -- Dosage forms , Analysis of variance
  • Description: The dissolution profiles of eight lots of paracetamol tablets representing seven different tablet brands are determined in a USP rotating basket assembly and a stationary basket-rotating paddle apparatus. The in vitro data are expressed in terms of dissolution parameters and inter-tablet differences are assessed statistically using analysis of variance (ANOVA) and the Scheffe test. Highly significant differences are observed between a number of the tablets at the 95% confidence level. Representative tablets from the dissolution rate study and a control dose of paracetamol dissolved in water are subsequently investigated in a 4 x 4 latin square design bioavailability trial. Serum and urine samples are collected and assayed for paracetamol alone (serum) and together with its metabolites (urine) by means of high pressure liquid chromatography. The in vivo data are expressed in terms of bioavailability parameters and differences between the test doses are assessed by means of ANOVA. No significant differences are observed between the dosage forms at the 95% confidence level.
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The morphology and biology of Anomala vetula Wied : an arthropod pest of turf in South Africa

  • Authors: Bradford, B
  • Date: 1946
  • Subjects: Anomala
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5903 , http://hdl.handle.net/10962/d1013545
  • Description: From Introduction: For the past eight years, the Zoology Department of Rhodes University College, in co-operation with African Explosives & Chemical Industries Ltd., has been studying certain entomological problems relating to turf on the golf course along the coastal belt of the Eastern Cape Province. The position is briefly as follows:- On the golf courses at Mossel Bay, Humewood (Port Elizabeth), Port Alfred and East London, vast damage has been done to the greens and fairways by "white grubs", the larval stages of Scarabaeidae. It would appear that these beetles had been present in the environs of the courses for many years, but it was only when large areas of the natural veld were converted into fairways, with a more or less uniform cover of grass, with Cynodon dactylon Pers. predominating, that conditions were inadvertently created which favoured the development of the beetles. It was not long after the establishment of these golf courses, that the beetles assumed the proportions of a pest, and the larvae began destroying the root system of the grasses covering the greens and fairways.
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The P.R.O. expert system shell

  • Authors: Bradshaw, John
  • Date: 1987 , 2013-04-03
  • Subjects: Expert systems (Computer science)
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4617 , http://hdl.handle.net/10962/d1006302 , Expert systems (Computer science)
  • Description: This thesis reports the research which led to the development of the P.R .O. Expert System Shell. The P.R.O . System is primarily, though not exclusively , designed for use in ecological domains. In the light of two specific expert systems, The RCS (River Conservation Status) and the Aquaculture Systems, which were developed as part of this research, a number of areas of importance have been identified. The most significant of these is the need to handle uncertainty effectively. The style of knowledge representation to be implemented also plays an important role. After consulting the relevant literature and the available microcomputer expert system shells, a number of ideas have been included in the P.R.O. System. The P.R.O . System is a backward chaining, production system based expert system shell. It embodies a simple but effective method of handling uncertainty. An important feature of this method is that it takes cognizance of the different relative importances of the conditions which need to be satisfied before a conclusion can be reached. The knowledge base consists of more than rules and questions. It also contains meta-knowledge, which is used by the inference engine. The P.R.O. System has been designed to be of practical use. Its strongest recommendations are therefore, that the two non-trivial systems which have been implemented in it, have been accepted by the experts and their peers as systems which produce good, accurate answers . , KMBT_363 , Adobe Acrobat 9.53 Paper Capture Plug-in
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A possible mechanism for enzymic depilation of skins

  • Authors: Brady, Dean
  • Date: 1989
  • Subjects: Chemistry, Technical , Tanning
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:3877 , http://hdl.handle.net/10962/d1001611
  • Description: Streptomyces fradiae is a bacterium which has been previously found to produce extracellular enzymes which are capable of wool degradation and skin depilation. Streptomyces fradiae 3739 and other strains of Streptomyces were found in this study to be able to degrade a keratin source (wool) to a considerable degree. However according to the evidence of SEM micrographs presented here the highly keratinised spindle cells of the paracortex are fairly resistant to protease attack, and it is the cementation material which binds these cells together which is initially degraded by the proteases. A large degree of correlation was found with the strains of Streptomyces studied, between the ability of the individual strains to degrade wool and the ability of their extracellular proteases to reduce the depilation load of sheepskins. With further analysis S. fradiae 3739 was found to produce at least one amylase and four or more proteases. The proteases as a group had maximal proteolytic activity in the 8.0-9.0 pH unit range, and were considerably thermostabilised by the inclusion of calcium ions into the reaction solution. The protease group was found to cause depilation of merino sheepskins. For comparative purposes a protease produced by a strain of Proteus vulgaris isolated from a staling hide with hair slip (natural depilation) was studied. The protease activity was maximal in the alkaline region between 8.0-9.0 pH units. Tbe protease appeared to be a single enzyme with a molecular mass of approximately 44 000 daltons. The protease was maximally active at 40°C, although it was only thermostable to 30°C. The enzyme was ineffectual as a depilant except when the skin was pre-treated with a strong alkali, preferably including sodium sulphite in the protease preparation. One of the most important differences between the extracellular proteases of S. fradiae and P. vulgaris was that the former were greater in variety and caused a greater decrease in the depilation load of sheepskins than the latter. Further research with mixtures of commercial proteases provided evidence that a synergistic depilatory effect occurs when proteases of complementary bond specificities are used in conjunction in enzymic depilatory preparations. Some form of strong alkali treatment of skins was found to be necessary to produce leather of the prerequisite quality when the skin was depilated by proteases, otherwise the skin was found to be depleted and stiff. Calcium hydroxide alone was found to be inadequate for this task, probably owing to the fact that it is less alkaline than the lime-sulphide mixture. The calcium hydroxide (lime) must therefore be used in conjunction with sodium hydroxide (which makes the solution as alkaline as that of the lime-sulphide solution) to produce leather comparable to that produced by the lime sulphide treatment. A combination of the information provided by the present research and that gleaned from the relevent literature allows for the construction of a model to represent the possible mechanism of enzymic depilation of skins, in which depilation is caused by the disruption of the basement membrane at the dermal-epidermal junction by the degradation of its constituent molecular components by general proteases, resulting in the removal of the epidermis and its associated wool or hair
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The effect of energy and protein nutritional levels on production of breeding ostriches

  • Authors: Brand, Zanell
  • Date: 2002
  • Subjects: Ostriches -- Nutrition , Ostriches -- Breeding--South Africa
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5805 , http://hdl.handle.net/10962/d1006312 , Ostriches -- Nutrition , Ostriches -- Breeding--South Africa
  • Description: Adequate and appropriate nutrition is essential for the production of high quality commercially farmed animal species. Although South Africa has had a well-established ostrich industry for over a century, little information on ostrich nutrition, in particular the specific nutritional requirements at different stages of production, is available. The industry has consequently relied heavily on data derived from poultry and pigs, but this has often proved to be unsatisfactory for ostriches and has resulted in serious nutritional-related problems. Recent studies on the metabolisable energy of specific components of diet formulations and balanced diets have indicated that ostriches have enhanced digestibility compared with poultry and pigs. At the same time, in the present economic climate, it is necessary to find cost-effective diets for breeding birds without compromising egg and chick production. This study primarily assessed the effect of different dietary protein and energy levels on production parameters and body condition of breeding female and male ostriches. The energy content of the diet appears to be the main constraint to egg production and breeders on low energy diets laid fewer eggs and lost more body condition compared with breeders fed higher energy diets. A diet with 8.5 MJ/kg DM and 10% protein with well balanced amino acids appears to be sufficient for female breeders without compromising production and a diet with 7.5 MJ/kg DM and 10% protein appears suitable for breeding male ostriches. Different dietary energy and protein levels similarly had little or no effect on egg quality and composition. This study also assessed the carry-over effect of the nutritional regime in one year on the production in the following breeding season. Females fed diets as low as 7.5 MJ/kg ME in the previous breeding season produced significantly fewer eggs in the next breeding season, which resulted in lower chick production. Energy had no effect on the percentage infertile eggs or on the initial egg weight. Different levels of dietary protein had no effect on egg production, egg weight, fertility, hatchability and initial chick weight.
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Models of internet connectivity for secondary schools in the Grahamstown circuit

The spectrographic determination of trace elements in citrus leaves

  • Authors: Brandt, Peter Jürgen
  • Date: 1962
  • Subjects: Citrus , Trace elements in plant nutrition
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4516 , http://hdl.handle.net/10962/d1013552
  • Description: From Introduction: With the rapidly growing knowledge on trace elements and their influence on plant nutrition the need for accurate and rapid methods for their determination arose. The essential plant nutrients are usually divided into two groups. The major or macronutrient elements, necessary in comparatively large amounts, and the trace or micro-nutrient elements. In the case of plants, the first group includes Sulphur, Phosphorus, Potassium, Magnesium, Calcium and Nitrogen. The essential trace elements are Iron, manganese, Boron, Copper, Zinc, Molybdenum and Chlorine. Cobalt is essential for animal nutrition as a constituent of Vitamin B₁₂, but its essentiality for plants has not yet been proved. The latter group consists of metals which are catalysts in enzyme reactions and whose presence in the plant in minute amount determines whether the plant will be able to complete the vegetative or reproductive stage of its life cycle. Molybdenum may be quoted as an example of an essential trace element. It is generally recognised to be the catalyst responsible for the fixation of Nitrogen.
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A review of archean orogenic gold deposits in greenstone belts and the Slave Province : exploration in the Yellowknife domain, NWT, Canada

  • Authors: Branson, Thomas Keegan
  • Date: 2014
  • Subjects: Gold ores -- Northwest Territories -- Yellowknife , Gold ores -- Geology , Gold mines and mining -- Northwest Territories -- Yellowknife , Greenstone belts -- Northwest Territories -- Yellowknife , Orogenic belts -- Northwest Territories -- Yellowknife , Prospecting -- Northwest Territories -- Yellowknife , Mining geology , Slave Province (N.W.T. and Nunavut)
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5055 , http://hdl.handle.net/10962/d1012142 , Gold ores -- Northwest Territories -- Yellowknife , Gold ores -- Geology , Gold mines and mining -- Northwest Territories -- Yellowknife , Greenstone belts -- Northwest Territories -- Yellowknife , Orogenic belts -- Northwest Territories -- Yellowknife , Prospecting -- Northwest Territories -- Yellowknife , Mining geology , Slave Province (N.W.T. and Nunavut)
  • Description: A review of Archean granite-greenstone terranes, orogenic gold deposits, the Slave Province and modern exploration tools, techniques and methods was conducted to identify prospective areas in the Yellowknife domain for hosting orogenic gold deposits and illustrate the best exploration methods for delineating this deposit type. This study identifies Archean granite-greenstone terranes as economically important hosts to quartz-carbonate vein-hosted orogenic gold deposits. These deposits occur at convergent plate margins, but can also be related to local extensional tectonics within a convergent setting. Heat generated from tectonic processes can trigger hydrothermal fluid movement along first-order faults and shear zones. Precipitation of gold-bearing quartz-carbonate veins from the hydrothermal fluids occurs in second- and third-order faults and shear zones related to the first-order structures. This study also identifies the Archean Slave Province in northern Canada as a well-endowed craton with numerous orogenic gold deposits, diamondiferous kimberlites, VMS deposits and several other mineralization styles. In particular, three greenstone belts (Yellowknife, Cameron River and Beaulieu River) associated with likely first-order structures are comprised of prospective rocks for hosting orogenic gold and VMS mineralization. The Yellowknife greenstone belt hosts the past-producing and former world-class Con and Giant orogenic gold deposits, but has been little explored with modern exploration techniques. The Cameron River and Beaulieu River greenstone belts host numerous base and precious metal VMS and BIF-hosted orogenic gold prospects and deposits, indicating mineralization is present. There is considerable potential for significant discoveries to be made using modern exploration techniques in the greenstone belts; however, exploration in the region has been hindered over the past decade by ongoing political negotiations. Once the political negotiations are finalized, application of modern exploration methods and techniques in the prospective greenstone belts should be carried out. Regional scale methodologies should be applied to generate targets using predictive modelling, implicit 3D modelling, 3D geochemistry and exploration targeting so decisions defining a businesses strategy for ground acquisition of high priority targets are made using quantitative analysis. Once ground is acquired, field-based exploration for orogenic gold and VMS deposits should include geological mapping with a focus on structural geology, geochemical sampling and airborne magnetic, radiometric and EM geophysical surveys. Prior to reconnaissance drilling, integration of all data layers and interpretation within a common 3D earth model should be conducted. Following successful reconnaissance drilling, definition drilling along strike and down dip of intersected mineralization, combined with borehole geophysics, should be carried out to delineate the extent of mineralization.
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The cheetahs of the Northern Tuli Game Reserve, Botswana: population estimates, monitoring techniques and human-predator conflict

  • Authors: Brassine, Eleanor I
  • Date: 2015
  • Subjects: Cheetah -- Botswana , Northern Tuli Game Reserve , Animal populations -- Botswana
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5922 , http://hdl.handle.net/10962/d1017800
  • Description: Remaining viable cheetah (Acinonyx jubatus) populations in Africa are threatened by direct persecution through conflict with farmers and habitat degradation and fragmentation. Botswana is considered a stronghold for free roaming cheetahs in Africa, yet the country has had relatively limited research on its cheetahs, and information from the east of the country is lacking. Data on the current status of populations is thus required to make informed management decisions. My study provides estimates of population density, abundance, distribution and status for the demographically open cheetah population of the Northern Tuli Game Reserve (NOTUGRE) in Botswana. The effectiveness of two population monitoring methods, namely camera trapping and a photographic survey, were also investigated. Moreover, I report on the level of conflict between livestock farmers and predators on rural communal farmlands within and adjacent to NOTUGRE. Data were collected between May 2012 and November 2013. Results indicate a low population density of 0.61 ± 0.18 adult cheetahs per 100 km² and a minimum population size of 10 individuals (nine adults and one cub). Camera traps placed at cheetah scent-marking posts increased detection rates and provided ideal set up locations. This approach, together with Spatial Explicit Capture- Recapture (SECR) models, is recommended for future studies. The long-term studies that are required to better understand the status of cheetahs in Botswana do not exist. Thus, photographic surveys may provide an alternative method for providing baseline data on population numbers, distribution and demography. The third aspect of my study gathered information on levels of livestock loss and human tolerance of predators through the use of interviews (n = 80). Conflict with subsistence farmers is a concern as livestock depredation is relatively high (9.1% of total livestock owned) and farmers had an overall negative attitude towards conservation of large predators. My results suggest that human-predator conflict in this area is more complex than the direct financial loss from depredation. Hence, reducing depredation rates alone is unlikely to change farmer tolerance of wildlife on farmlands. Improved, responsible farm management, including self-responsibility for livestock rearing, and positive appreciation for wildlife are necessary. The NOTUGRE cheetah population requires further research to understand possible threats to the population. Furthermore, a better understanding of the connectivity between cheetahs of NOTUGRE, South Africa and Zimbabwe is required. The number of cheetahs within NOTUGRE is too small to sustain a viable population, hence conserving cheetahs outside of the protected area should be a priority for the conservation of the population. This can only be achieved through assistance and involvement from national authorities, local people and conservation organisations.
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The diet and ecological role of black-backed jackals, Canis Mesomelas, in two conservation areas in the Eastern Cape Province, South Africa

  • Authors: Brassine, Mathilde Chloé
  • Date: 2012
  • Subjects: Black-backed jackal -- South Africa -- Eastern Cape , Jackals -- South Africa -- Eastern Cape , Jackals -- Food , Wildlife conservation
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5660 , http://hdl.handle.net/10962/d1005344 , Black-backed jackal -- South Africa -- Eastern Cape , Jackals -- South Africa -- Eastern Cape , Jackals -- Food , Wildlife conservation
  • Description: Mammalian predators are keystone species and can have disproportionately large impacts, relative to their abundance, on the communities and ecosystems they inhabit. In addition, predator-prey interactions (including bottom-up and top-down effects) are the fundamental linkage among species in food webs. Thus, understanding the food requirements of predators is imperative. There have been no comprehensive studies on the diet of black-backed jackals (Canis mesomelas) across a full seasonal cycle in the Albany Thicket Biome of the Eastern Cape Province. This biome is rich in plant diversity, is highly nutritious for herbivores and supports a large number of mammalian species, yet it is poorly conserved. The aim of the present study was to establish the diet of black-backed jackals on two conservation areas where thicket vegetation dominates (Great Fish River Reserve and Kwandwe Private Game Reserve). The diet was assessed using scats collected monthly from June 2009 to May 2010 and a sample of 25 stomachs over the same period. At both sites and across all seasons, mammalian hair was encountered most often (93% of all scats) and a total of 23 mammal species were consumed. Of the mammals recorded in the scats, hair from several ungulate species was encountered most frequently and these were mostly hider species (offspring hidden in tall vegetation just after birth). On a seasonal basis, the proportion of mammalian hair was lowest in autumn. However, the contribution of fruits (Carissa haematocarpa, Diospyros lycioides and Grewia robusta) to the diet was significantly higher during this season. This suggests that black-backed jackals may employ diet switching in the Eastern Cape as a mechanism to capitalise on seasonally abundant food sources. Based on the stomach content analysis, invertebrates were consumed as frequently as mammalian prey throughout the year but mammalian prey was recorded in greater quantity (i.e. volume and mass). Thus, scat analysis may have overestimated mammalian prey and fruits and underestimated the importance of invertebrates, particularly highly digestible species (e.g. termites). In spite of these differences, scat analysis demonstrated similar general and seasonal trends as the stomach content analysis. Thus, scat analysis can provide an efficient and relatively accurate source of information on the diet of blackbacked jackals in the Eastern Cape. Both stomach content and scat collection methods depicted the black-backed jackal as a generalist and opportunistic predator, mainly relying on mammals and invertebrates. Although the results of my study suggest that black-backed jackal may be important predators of certain ungulate species, further study on their prey preferences, actual biomass ingested and the effects on the population viability of prey species is required in order to develop a robust management plan for the species in the protected areas of the Eastern Cape.
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Gold exploration in tropical and sub-tropical terrains with special emphasis on Central and Western Africa

  • Authors: Breedt, Machiel Christoffel
  • Date: 1996
  • Subjects: Geochemical prospecting -- Tropics , Geochemical prospecting -- Africa , Gold ores -- Geology -- Tropics , Gold ores -- Geology -- Africa
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4966 , http://hdl.handle.net/10962/d1005578 , Geochemical prospecting -- Tropics , Geochemical prospecting -- Africa , Gold ores -- Geology -- Tropics , Gold ores -- Geology -- Africa
  • Description: The aim of this dissertation is an attempt to' provide a general guide for future gold exploration in tropical and sub-tropical terrains. The dissertation includes a brief discussion of the various exploration techniques used in regional and local exploration. This provide the necessary background knowledge to discriminate between the constraints and applications and to be able to select the techniques which are more suitable for gold exploration in tropical and sub-tropical terrains. Weathering, gold geochemistry and soil formation, fields often neglected, are emphasized to illustrate the importance of the mobility and dispersion of gold in the weathering of the lateritic soil profile. A sound knowledge and experience in regolith mapping is to the advantage of the explorationist. Case studies with special emphasis on Central- and Western Africa are included to illustrate the effectiveness of some of the gold exploration techniques in tropical and sub-tropical terrains. Gold exploration is a highly complex and demanding science and to be successfull involves the full intergration of all geological, geochemical and geophysical information available. An intergrated exploration method and strategy would enhance the possibility of making viable discoveries in this highly competative environment where our mineral resources become more depleted every day. Where applicable, the reader is refered to various recommended literature sources to provide the necessary background knowledge which form an integral part of gold exploration.
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The effect of varying heavy metal balances in the nutrient medium, on the growth and development of Aspergillus Sp. and Penicillium Sp.

  • Authors: Breen, C M
  • Date: 1965
  • Subjects: Heavy metals -- Absorption and adsorption , Aspergillus -- Growth , Penicillium -- Growth , Fungi -- Nutrition
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4268 , http://hdl.handle.net/10962/d1014597
  • Description: The study was conducted to investigate the effects of: (i) varying the level of supply of the heavy metals iron, manganese, copper and zinc. (ii) varying the ratio between different pairs of metals in the medium. In particular the iron:manganese and zinc:copper ratios were studied. Initially the two fungi Aspergillus niger van Tieghem, (variety and strain) and Penicillium notatum Westling, were used. Penicillium notatum Westling was subsequently discarded, in favour of Penicillium glancum Link, because it did not sporulate freely in liquid culture. The fungi were grown in controlled nutrient solutions, and during the course of tho growth and development, the form and sporulation of the felts was noted. After a period of growth, the felts were removed, dried and weighed. The pH of the liquor was measured. the results were studied to determine the effect of varying levels of supply of the heavy metals, and of the varying heavy metal ratios in the culture solution. In the investigation of the effect of varying the level of supply of individual heavy metals, optimum concentrations were demonstrated for copper and manganese. Increaning the concentration of pairs of heavy metals cimultaneously was found to influence the appearance and degree of symptoms of toxicity. Cultures of Aspergillus and Penicillium were found to be able to tolerate concentrations of copper, in particular, considerably greater than the observed optima, when zinc was present in equal concentration. Citric acid, and subsequently ethylene- diaminetetra-acetic acid, were used as chelating agents, in order to prevent the precipitation of the metals in the culture solution during autoclaving. It was found that the use of chelating agents markedly reduced symptoms of toxicity. There was no conclusive evidence that the iron:manganese ratio in the culture medium was an important factor in the growth and development of cultures of Aspergillus and Penicillium. However there is considerable evidence that in cultures of Penicillium, the zinc:copper ratio in the medium is of some importance in the determination of the dry weight yield trends. This effect was not demonstrated in cultures of Aspergillus.
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The genesis of ilmenite-rich heavy mineral deposits in the Bothaville/Delmas area, and an economic analysis of titanium, with particular reference to the Dwarsfontein deposit, Delmas district

  • Authors: Brennan, Michael Brendan
  • Date: 1991
  • Subjects: Heavy minerals -- South Africa -- Transvaal , Titanium , Geology -- South Africa -- Transvaal
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4949 , http://hdl.handle.net/10962/d1005561 , Heavy minerals -- South Africa -- Transvaal , Titanium , Geology -- South Africa -- Transvaal
  • Description: A number of ilmenite-rich heavy mineral deposits occur along the northern margin of the intracratonic Karoo basin, and are hosted by the fluvio-deltaic Middle Ecca Group. Coastal reworking of delta front sands within a regressive, microtidal shoreline may be considered as a depositional model for the development of the heavy mineral deposits. An economic analysis of titanium suggests long term positive demand, and sustained high prices for this commodity. An evaluation of the Dwarsfontein ilmenite-rich heavy mineral deposit, using available data, indicates how important it is for deposits of this type to be situated close to an upgrading plant or export harbour.
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Voltammetric analysis of pesticides and their degradation: A case study of Amitraz and its degradants

  • Authors: Brimecombe, Rory Dennis
  • Date: 2006
  • Subjects: Hydrolysis , Biodegradation , Voltammetry , Pesticides -- Biodegradation , Pesticides -- Environmental aspects , Acaricides , Acaricides -- Physiological effect
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4131 , http://hdl.handle.net/10962/d1015724
  • Description: Amitraz is a formamide acaricide used predominantly in the control of ectoparasites in livestock and honeybees. Amitraz hydrolysis is rapid and occurs under acidic conditions, exposure to sunlight and biodegradation by microorganisms. The main hydrolysis product of amitraz, 2,4-dimethylaniline, is recalcitrant in the environment and toxic to humans. An electrochemical method for the determination of total amitraz residues and its final breakdown product, 2,4-dimethylaniline, in spent cattle dip, is presented. Cyclic voltammetry at a glassy carbon electrode showed the irreversible oxidation of amitraz and 2,4-dimethylaniline. A limit of detection in the range of 8.5 x 10⁻⁸ M for amitraz and 2 x 10⁻⁸ M for 2,4-dimethylaniline was determined using differential pulse voltammetry. Feasibility studies in which the effect of supporting electrolyte type and pH had on electroanalysis of amitraz and its degradants, showed that pH affects current response as well as the potential at which amitraz and its degradants are oxidised. Britton-Robinson buffer was found to be the most suitable supporting electrolyte for detection of amitraz and its degradants in terms of sensitivity and reproducibility. Studies performed using environmental samples showed that the sensitivity and reproducibility of amitraz and 2,4-dimethylaniline analyses in spent cattle dip were comparable to analyses of amitraz and 2,4-dimethylaniline performed in Britton-Robinson buffer. In addition, the feasibility qf measuring amitraz and 2,4-dimethylaniline in environmental samples was assessed and compared to amitraz and 2,4-dimethylaniline analyses in Britton-Robinson buffer. Amitraz and 2,4-dimethylaniline were readily detectable in milk and honey. Furthermore, it was elucidated that 2,4-dimethylaniline can be metabolised to 3-methylcatechol by Pseudomonas species and the proposed breakdown pathway is presented. The biological degradation of amitraz and subsequent formation of 2,4-dimethylaniline was readily monitored in spent cattle dip. The breakdown of amitraz to 2,4-dimethylaniline and then to 3-MC was monitored using cyclic voltammetry.
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The selection and evaluation of grey-level thresholds applied to digital images

  • Authors: Brink, Anton David
  • Date: 1988
  • Subjects: Image processing -- Digital techniques
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5443 , http://hdl.handle.net/10962/d1001996
  • Description: Many applications of image processing require the initial segmentation of the image by means of grey-level thresholding. In this thesis, the problems of automatic threshold selection and evaluation are addressed in order to find a universally applicable thresholding method. Three previously proposed threshold selection techniques are investigated, and two new methods are introduced. The results of applying these methods to several different images are evaluated using two threshold evaluation techniques, one subjective and one quantitative. It is found that no threshold selection technique is universally acceptable, as different methods work best with different images and applications
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Remarks on formalized arithmetic and subsystems thereof

  • Authors: Brink, C
  • Date: 1975
  • Subjects: Gödel, Kurt , Logic, Symbolic and mathematical , Semantics (Philosophy) , Arithmetic -- Foundations , Number theory
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5424 , http://hdl.handle.net/10962/d1009752 , Gödel, Kurt , Logic, Symbolic and mathematical , Semantics (Philosophy) , Arithmetic -- Foundations , Number theory
  • Description: In a famous paper of 1931, Gödel proved that any formalization of elementary Arithmetic is incomplete, in the sense that it contains statements which are neither provable nor disprovable. Some two years before this, Presburger proved that a mutilated system of Arithmetic, employing only addition but not multiplication, is complete. This essay is partly an exposition of a system such as Presburger's, and partly an attempt to gain insight into the source of the incompleteness of Arithmetic, by linking Presburger's result with Gödel's.
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Re-surveying the insectivorous bats of northern Kruger National Park, South Africa

  • Authors: Brinkley, Erin Reed
  • Date: 2019
  • Subjects: Bats -- South Africa , Bats -- Behavior , Bats -- South Africa -- Ecology
  • Language: English
  • Type: text , Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10962/115697 , vital:34216
  • Description: With over 1,300 known species, the order Chiroptera makes up about 20 percent of all mammalian species. Due to its sub-tropical climate, the northern part of Kruger National Park (KNP) in South Africa is believed to have the highest bat species richness in the country. However, the last comprehensive assessment of bat diversity in the region was conducted more than 30 years ago. In 2017 and 2018, I undertook the first detailed re-assessment of the bat communities of the northern KNP since the early 1980’s. I used both live-capture (harp traps and mist-netting) and acoustic technology (SM2 and SM4 Songmeters, Wildlife Acoustics. MA, USA) to sample bats at 24 sites across the northern region of KNP. Through live-capture (336 trapping hours), 155 bats representing 13 species from five families were recorded including Cloetis pervicali, which has never been recorded within the borders of the KNP before. The echolocation calls of all captured bats were recorded to develop a site-specific call reference library that was used (in combination with existing reference calls) as a guide for the identification of bat calls recorded using the acoustic detectors set across 24 sites (278 sampling nights). The acoustic monitoring identified 22 species from six families and two unknown sets of calls. Compared to the historical data of 40 documented species (collected over a 30-year period), the current survey (27 species) resulted in a lower species richness. However, this is likely due to the lower overall sampling effort during my survey. By re-surveying the bats of northern KNP, I have contributed towards an overall bat species inventory for this region. In addition, I have generated an important baseline dataset for the future monitoring of bat diversity across the KNP. Due to bats being important biological indicators, increased research on the various species and their behaviours is essential for improving our understanding of climate change effects as well as the overall health of the environment, especially in protected areas.
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Proportional distribution of predominant rumen bacteria between the solid and the liquid portions of ruminal ingesta

  • Authors: Brinkman, Paul A
  • Date: 1966
  • Subjects: Rumen -- Microbiology , Bacteria -- Rumen
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4100 , http://hdl.handle.net/10962/d1009715 , Rumen -- Microbiology , Bacteria -- Rumen
  • Description: That certain bacteria in the rumen of sheep and cattle are attached to solid particles in the ruminal ingesta has been known for many years. In 1942 Baker published direct microscopical evidence that bacteria were attached to cellulose food particles and to starch granules in the rumen. The sites of attachment of these bacteria corresponded to sites of disintegration of the particles when viewed by polarised light. This indicated that at least bacteria attacking solid substrates such as cellulose and starch were attached to particles of ruminal ingesta. Van der Wath (1942) found rumen bacteria attached to particles of chemically pure cellulose and of crushed maize which he suspended in separate compartments of a pure silk bag inside the rumen of sheep. The bacteria associated with the particles of cellulose were mainly Gram negative rods , while clusters of iodophilic cocci were observed in most instances around the maize kernels . The latter organisms were isolated in pure culture and found to be heat-tolerant, short-chain, Gram positive cocci fermenting glucose, maltose, and other soluble sugars as well as starch. It was thus not surprising that many years later Schwartz et al (1964) obtained evidence which suggested that bacteria metabolising soluble substrates such as glucose also showed marked attachment to solid particles of ingesta.
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