The association between HIV tri-therapy with the development of Type-2 Diabetes Mellitus in a rural South African District: A case-control study
- Nokwanda E. Bam, , Sikhumbuzo A. Mabunda, Jafta Ntsaba1, Teke Apalata, Sibusiso C. Nomatshila4, Wezile Chitha
- Authors: Nokwanda E. Bam , , Sikhumbuzo A. Mabunda , Jafta Ntsaba1 , Teke Apalata , Sibusiso C. Nomatshila4 , Wezile Chitha
- Date: 2020
- Language: English
- Type: Journal article
- Identifier: http://hdl.handle.net/11260/4008 , vital:43983
- Full Text:
- Authors: Nokwanda E. Bam , , Sikhumbuzo A. Mabunda , Jafta Ntsaba1 , Teke Apalata , Sibusiso C. Nomatshila4 , Wezile Chitha
- Date: 2020
- Language: English
- Type: Journal article
- Identifier: http://hdl.handle.net/11260/4008 , vital:43983
- Full Text:
The association between HIV tri-therapy with the development of Type-2 Diabetes Mellitus in a rural South African District: A case-control study
- Nokwanda E. Bam, Teke Apalata, Sikhumbuzo A. Mabunda, Jafta Ntsaba, Sibusiso C. Nomatshila, Wezile Chitha
- Authors: Nokwanda E. Bam , Teke Apalata , Sikhumbuzo A. Mabunda , Jafta Ntsaba , Sibusiso C. Nomatshila , Wezile Chitha
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4667 , vital:44155
- Full Text:
- Authors: Nokwanda E. Bam , Teke Apalata , Sikhumbuzo A. Mabunda , Jafta Ntsaba , Sibusiso C. Nomatshila , Wezile Chitha
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4667 , vital:44155
- Full Text:
Socio-demographic associations of HIV among women attending antenatal care in selected rural primary care facilities in South Africa’s Eastern Cape province
- Sikhumbuzo A. Mabunda, Khuthala Sigovana, Wezile Chitha, Teke Apalata, Sibusiso Nomatshila
- Authors: Sikhumbuzo A. Mabunda , Khuthala Sigovana , Wezile Chitha , Teke Apalata , Sibusiso Nomatshila
- Date: 2021
- Language: English
- Type: Journal article
- Identifier: http://hdl.handle.net/11260/4112 , vital:44025
- Full Text:
- Authors: Sikhumbuzo A. Mabunda , Khuthala Sigovana , Wezile Chitha , Teke Apalata , Sibusiso Nomatshila
- Date: 2021
- Language: English
- Type: Journal article
- Identifier: http://hdl.handle.net/11260/4112 , vital:44025
- Full Text:
In vitro antimicrobial photodynamic inactivation of multidrugresistant Acinetobacter baumannii biofilm using Protoporphyrin IX and Methylene blue
- Yaw Adjei Anane, Teke Apalata, Sandeep Vasaikar, Grace Emily Okuthe, Sandile Phinda Songca
- Authors: Yaw Adjei Anane , Teke Apalata , Sandeep Vasaikar , Grace Emily Okuthe , Sandile Phinda Songca
- Date: 2020
- Language: English
- Identifier: http://hdl.handle.net/11260/2273 , vital:41315
- Full Text:
- Authors: Yaw Adjei Anane , Teke Apalata , Sandeep Vasaikar , Grace Emily Okuthe , Sandile Phinda Songca
- Date: 2020
- Language: English
- Identifier: http://hdl.handle.net/11260/2273 , vital:41315
- Full Text:
Molecular Detection of Carbapenemase-Encoding Genes in Multidrug-Resistant Acinetobacter baumannii Clinical Isolates in South Africa
- Yaw Adjei Anane, Teke Apalata, Sandeep Vasaikar, Grace Emily Okuthe, Sandile Songca
- Authors: Yaw Adjei Anane , Teke Apalata , Sandeep Vasaikar , Grace Emily Okuthe , Sandile Songca
- Date: 2020
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2263 , vital:41314
- Description: https://www.hindawi.com/journals/ijmicro/2020/7380740/
- Full Text:
- Authors: Yaw Adjei Anane , Teke Apalata , Sandeep Vasaikar , Grace Emily Okuthe , Sandile Songca
- Date: 2020
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2263 , vital:41314
- Description: https://www.hindawi.com/journals/ijmicro/2020/7380740/
- Full Text:
Detection of extended spectrum beta‑lactamase genes in Pseudomonas aeruginosa isolated from patients in rural Eastern Cape Province, South Africa
- Mojisola C. Hosu, Sandeep D. Vasaikar,, Grace E. Okuthe, Teke Apalata
- Authors: Mojisola C. Hosu , Sandeep D. Vasaikar, , Grace E. Okuthe , Teke Apalata
- Date: 2021
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4509 , vital:44128
- Description: The proliferation of extended spectrum beta-lactamase (ESBL) producing Pseudomonas aeruginosa represent a major public health threat. In this study, we evaluated the antimicrobial resistance patterns of P. aeruginosa strains and characterized the ESBLs and Metallo- β-lactamases (MBL) produced. Strains of P. aeruginosa cultured from patients who attended Nelson Mandela Academic Hospital and other clinics in the four district municipalities of the Eastern Cape between August 2017 and May 2019 were identified; antimicrobial susceptibility testing was carried out against thirteen clinically relevant antibiotics using the BioMérieux VITEK 2 and confirmed by Beckman autoSCAN-4 System. Real-time PCR was done using Roche Light Cycler 2.0 to detect the presence of ESBLs; blaSHV, blaTEM and blaCTX-M genes; and MBLs; blaIMP, blaVIM. Strains of P. aeruginosa demonstrated resistance to wide-ranging clinically relevant antibiotics including piperacillin (64.2%), followed by aztreonam (57.8%), cefepime (51.5%), ceftazidime (51.0%), piperacillin/tazobactam (50.5%), and imipenem (46.6%). A total of 75 (36.8%) multidrug-resistant (MDR) strains were observed of the total pool of isolates. The blaTEM, blaSHV and blaCTX-M was detected in 79.3%, 69.5% and 31.7% isolates (n = 82), respectively. The blaIMP was detected in 1.25% while no blaVIM was detected in any of the strains tested. The study showed a high rate of MDR P. aeruginosa in our setting. The vast majority of these resistant strains carried blaTEM and blaSHV genes. Continuous monitoring of antimicrobial resistance and strict compliance towards infection prevention and control practices are the best defence against spread of MDR P. aeruginosa.
- Full Text:
- Authors: Mojisola C. Hosu , Sandeep D. Vasaikar, , Grace E. Okuthe , Teke Apalata
- Date: 2021
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4509 , vital:44128
- Description: The proliferation of extended spectrum beta-lactamase (ESBL) producing Pseudomonas aeruginosa represent a major public health threat. In this study, we evaluated the antimicrobial resistance patterns of P. aeruginosa strains and characterized the ESBLs and Metallo- β-lactamases (MBL) produced. Strains of P. aeruginosa cultured from patients who attended Nelson Mandela Academic Hospital and other clinics in the four district municipalities of the Eastern Cape between August 2017 and May 2019 were identified; antimicrobial susceptibility testing was carried out against thirteen clinically relevant antibiotics using the BioMérieux VITEK 2 and confirmed by Beckman autoSCAN-4 System. Real-time PCR was done using Roche Light Cycler 2.0 to detect the presence of ESBLs; blaSHV, blaTEM and blaCTX-M genes; and MBLs; blaIMP, blaVIM. Strains of P. aeruginosa demonstrated resistance to wide-ranging clinically relevant antibiotics including piperacillin (64.2%), followed by aztreonam (57.8%), cefepime (51.5%), ceftazidime (51.0%), piperacillin/tazobactam (50.5%), and imipenem (46.6%). A total of 75 (36.8%) multidrug-resistant (MDR) strains were observed of the total pool of isolates. The blaTEM, blaSHV and blaCTX-M was detected in 79.3%, 69.5% and 31.7% isolates (n = 82), respectively. The blaIMP was detected in 1.25% while no blaVIM was detected in any of the strains tested. The study showed a high rate of MDR P. aeruginosa in our setting. The vast majority of these resistant strains carried blaTEM and blaSHV genes. Continuous monitoring of antimicrobial resistance and strict compliance towards infection prevention and control practices are the best defence against spread of MDR P. aeruginosa.
- Full Text:
Prevalence and molecular analysis of multidrug-resistant Acinetobacter baumannii in the extra-hospital environment in Mthatha, South Africa
- Yaw Anane A, Teke Apalata, Sandeep Vasaikar, Grace Emily Okuthe, Sandile Songca
- Authors: Yaw Anane A , Teke Apalata , Sandeep Vasaikar , Grace Emily Okuthe , Sandile Songca
- Date: 2019
- Subjects: Acinetobacter baumannii Carbapenemase-encoding genes Extra-hospital Multidrug-resistance ISAba1 intI1
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2409 , vital:41457
- Description: Introduction: The presence of Acinetobacter baumannii outside hospitals remains unclear. This study aimed to determine the prevalence of multidrug-resistance (MDR) A. baumannii in the extra-hospital environment in Mthatha, South Africa and to investigate the frequency of carbapenemase-encoding genes. Material and Methods: From August 2016 to July 2017 a total of 598 abattoir samples and 689 aquatic samples were collected and analyzed presumptively by cultural methods for the presence of A. baumannii using CHROMagarTM Acinetobacter medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL) and confirmed by the detection of their intrinsic blaOXA-51 gene. Confirmed MDR A. baumannii isolates were screened for the presence of carbapenemase-encoding genes, ISAba1 insertion sequence and integrase intI1. Results: In total, 248 (19.3%) Acinetobacter species were isolated. Acinetobacter. baumannii was detected in 183 (73.8%) of which 85 (46.4%) and 98 (53.6%) were recovered from abattoir and aquatic respectively. MDR A. baumannii was detected in 56.5% (48/85) abattoir isolates and 53.1% (52/98) aquatic isolates. Isolates showed high resistance to antimicrobials most frequently used to treat Acinetobacter infections such as piperacillin/tazobactam; abattoir (98% of isolates resistant), aquatic (94% of isolates resistant), ceftazidime (84%, 83%), ciprofloxacin (71%, 70%), amikacin (41%, 42%), imipenem (75%, 73%), and meropenem (74%, 71%). All the isolates were susceptible to tigecycline and colistin. All the isolates carried blaOXA-51-like. The blaOXA-23 was detected in 32 (66.7%) abattoir isolates and 11 (21.2%) aquatic isolates. The blaOXA-58-like was positive in 7 (14.6%) and 4 (7.7%) abattoir and aquatic isolates, respectively. Both groups of isolates lacked blaOXA-24-like, blaIMP-type, blaVIM-type, blaNDM-1, blaSIM, blaAmpC, ISAba1 and inI1. Isolates showed high level of Multiple Antibiotic Resistance Index (MARI) ranging from 0.20-0.52. Conclusion: Extra-hospital sources such as abattoir and aquatic environments may be a vehicle of spread of MDR A. baumannii strains in the community and hospital settings
- Full Text:
- Authors: Yaw Anane A , Teke Apalata , Sandeep Vasaikar , Grace Emily Okuthe , Sandile Songca
- Date: 2019
- Subjects: Acinetobacter baumannii Carbapenemase-encoding genes Extra-hospital Multidrug-resistance ISAba1 intI1
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2409 , vital:41457
- Description: Introduction: The presence of Acinetobacter baumannii outside hospitals remains unclear. This study aimed to determine the prevalence of multidrug-resistance (MDR) A. baumannii in the extra-hospital environment in Mthatha, South Africa and to investigate the frequency of carbapenemase-encoding genes. Material and Methods: From August 2016 to July 2017 a total of 598 abattoir samples and 689 aquatic samples were collected and analyzed presumptively by cultural methods for the presence of A. baumannii using CHROMagarTM Acinetobacter medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL) and confirmed by the detection of their intrinsic blaOXA-51 gene. Confirmed MDR A. baumannii isolates were screened for the presence of carbapenemase-encoding genes, ISAba1 insertion sequence and integrase intI1. Results: In total, 248 (19.3%) Acinetobacter species were isolated. Acinetobacter. baumannii was detected in 183 (73.8%) of which 85 (46.4%) and 98 (53.6%) were recovered from abattoir and aquatic respectively. MDR A. baumannii was detected in 56.5% (48/85) abattoir isolates and 53.1% (52/98) aquatic isolates. Isolates showed high resistance to antimicrobials most frequently used to treat Acinetobacter infections such as piperacillin/tazobactam; abattoir (98% of isolates resistant), aquatic (94% of isolates resistant), ceftazidime (84%, 83%), ciprofloxacin (71%, 70%), amikacin (41%, 42%), imipenem (75%, 73%), and meropenem (74%, 71%). All the isolates were susceptible to tigecycline and colistin. All the isolates carried blaOXA-51-like. The blaOXA-23 was detected in 32 (66.7%) abattoir isolates and 11 (21.2%) aquatic isolates. The blaOXA-58-like was positive in 7 (14.6%) and 4 (7.7%) abattoir and aquatic isolates, respectively. Both groups of isolates lacked blaOXA-24-like, blaIMP-type, blaVIM-type, blaNDM-1, blaSIM, blaAmpC, ISAba1 and inI1. Isolates showed high level of Multiple Antibiotic Resistance Index (MARI) ranging from 0.20-0.52. Conclusion: Extra-hospital sources such as abattoir and aquatic environments may be a vehicle of spread of MDR A. baumannii strains in the community and hospital settings
- Full Text:
Molecular Detection of Carbapenemase-Encoding Genes in Multidrug-Resistant Acinetobacter baumannii Clinical Isolates in South Africa
- Yaw Adjei Anane, Grace Emily Okuthe, Teke Apalata, Sandeep Vasaikar, Sandile Songca
- Authors: Yaw Adjei Anane , Grace Emily Okuthe , Teke Apalata , Sandeep Vasaikar , Sandile Songca
- Date: 2020
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4351 , vital:44108
- Full Text:
- Authors: Yaw Adjei Anane , Grace Emily Okuthe , Teke Apalata , Sandeep Vasaikar , Sandile Songca
- Date: 2020
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4351 , vital:44108
- Full Text:
Molecular Detection of Antibiotic-Resistant Genes in Pseudomonas aeruginosa from Nonclinical Environment: Public Health Implications in Mthatha, Eastern Cape Province, South Africa
- Mojisola Clara Hosu, Sandeep Vasaikar, Grace Emily Okuthe, Teke Apalata
- Authors: Mojisola Clara Hosu , Sandeep Vasaikar , Grace Emily Okuthe , Teke Apalata
- Date: 5 January 2021
- Subjects: Microbiology
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2417 , vital:41877
- Description: Evaluation of resistant profiles and detection of antimicrobial-resistant genes of bacterial pathogens in the nonclinical milieu is imperative to assess the probable risk of dissemination of resistant genes in the environment. .is paper sought to identify antibiotic-resistant genes in Pseudomonas aeruginosa from nonclinical sources in Mthatha, Eastern Cape, and evaluate its public health implications. Samples collected from abattoir wastewater and aquatic environment were processed by membrane filtration and cultured on CHROMagarTM Pseudomonas medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL). Molecular characterization of the isolates was confirmed using real-time polymerase chain reaction (rPCR) and selected isolates were further screened for the possibility of harboring antimicrobial resistance genes. Fifty-one Pseudomonas species were recovered from abattoir wastewater and surface water samples, out of which thirty-six strains were Pseudomonas aeruginosa (70.6%). .e P. aeruginosa isolates demonstrated resistance to aztreonam (86.1%), ceftazidime (63.9%), piperacillin (58.3%), cefepime (55.6%), imipenem (50%), piperacillin/tazobactam (47.2%), meropenem (41.7%), and levofloxacin (30.6%). Twenty out of thirty-six P. aeruginosa displayed multidrug resistance profiles and were classified as multidrug-resistant (MDR) (55.6%). Most of the bacterial isolates exhibited a high Multiple Antibiotic Resistance (MAR) Index ranging from 0.08 to 0.69 with a mean MAR index of 0.38. In the rPCR analysis of fifteen P. aeruginosa isolates, 14 isolates (93.3%) were detected harboring blaSHV, six isolates (40%) harbored blaTEM, and three isolates (20%) harbored blaCTX-M, being the least occurring ESBL. Results of the current study revealed that P. aeruginosa isolates recovered from nonclinical milieu are resistant to frontline clinically relevant antipseudomonal drugs. .is is concerning as it poses a risk to the environment and constitutes a public health threat. Given the public health relevance, the paper recommends monitoring of multidrug-resistant pathogens in effluent environments.
- Full Text:
- Authors: Mojisola Clara Hosu , Sandeep Vasaikar , Grace Emily Okuthe , Teke Apalata
- Date: 5 January 2021
- Subjects: Microbiology
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2417 , vital:41877
- Description: Evaluation of resistant profiles and detection of antimicrobial-resistant genes of bacterial pathogens in the nonclinical milieu is imperative to assess the probable risk of dissemination of resistant genes in the environment. .is paper sought to identify antibiotic-resistant genes in Pseudomonas aeruginosa from nonclinical sources in Mthatha, Eastern Cape, and evaluate its public health implications. Samples collected from abattoir wastewater and aquatic environment were processed by membrane filtration and cultured on CHROMagarTM Pseudomonas medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL). Molecular characterization of the isolates was confirmed using real-time polymerase chain reaction (rPCR) and selected isolates were further screened for the possibility of harboring antimicrobial resistance genes. Fifty-one Pseudomonas species were recovered from abattoir wastewater and surface water samples, out of which thirty-six strains were Pseudomonas aeruginosa (70.6%). .e P. aeruginosa isolates demonstrated resistance to aztreonam (86.1%), ceftazidime (63.9%), piperacillin (58.3%), cefepime (55.6%), imipenem (50%), piperacillin/tazobactam (47.2%), meropenem (41.7%), and levofloxacin (30.6%). Twenty out of thirty-six P. aeruginosa displayed multidrug resistance profiles and were classified as multidrug-resistant (MDR) (55.6%). Most of the bacterial isolates exhibited a high Multiple Antibiotic Resistance (MAR) Index ranging from 0.08 to 0.69 with a mean MAR index of 0.38. In the rPCR analysis of fifteen P. aeruginosa isolates, 14 isolates (93.3%) were detected harboring blaSHV, six isolates (40%) harbored blaTEM, and three isolates (20%) harbored blaCTX-M, being the least occurring ESBL. Results of the current study revealed that P. aeruginosa isolates recovered from nonclinical milieu are resistant to frontline clinically relevant antipseudomonal drugs. .is is concerning as it poses a risk to the environment and constitutes a public health threat. Given the public health relevance, the paper recommends monitoring of multidrug-resistant pathogens in effluent environments.
- Full Text:
Prevalence of Hypertension and Its Associated Risk Factors in a Rural Black Population of Mthatha Town, South Africa
- Jyoti Rajan Sharma, Sihle E. Mabhida, Bronwyn Myers 3, Teke Apalata, Edward Nicol, Mongi Benjeddou, Christo Muller, Rabia Johnson
- Authors: Jyoti Rajan Sharma , Sihle E. Mabhida , Bronwyn Myers 3 , Teke Apalata , Edward Nicol , Mongi Benjeddou , Christo Muller , Rabia Johnson
- Date: 2021
- Language: English
- Type: Journal article
- Identifier: http://hdl.handle.net/11260/3980 , vital:43979
- Full Text:
- Authors: Jyoti Rajan Sharma , Sihle E. Mabhida , Bronwyn Myers 3 , Teke Apalata , Edward Nicol , Mongi Benjeddou , Christo Muller , Rabia Johnson
- Date: 2021
- Language: English
- Type: Journal article
- Identifier: http://hdl.handle.net/11260/3980 , vital:43979
- Full Text:
The Role of T Helper 17 (Th17) and Regulatory T Cells (Treg) in the Pathogenesis of Vulvovaginal Candidiasis among HIV-Infected Women
- Teke Apalata, Benjamin Longo-Mbenza
- Authors: Teke Apalata , Benjamin Longo-Mbenza
- Date: 2020
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4363 , vital:44109
- Full Text:
- Authors: Teke Apalata , Benjamin Longo-Mbenza
- Date: 2020
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4363 , vital:44109
- Full Text:
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