Effects of supply chain disruptions on alcohol consumption behaviour in a South African township
- Authors: Sipamla, Noluthando
- Date: 2024-12
- Subjects: Business logistics , Inventory control , Alcoholism
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/69913 , vital:78172
- Description: This study investigated the effect of supply chain disruptions on alcohol consumer consumption behaviour in the Eastern Cape Province, but with the specific reference to Mdantsane Township in the Buffalo City Municipality. A quantitative methodology was employed, and self-administered questionnaires were used to collect data. The results of the study revealed that: disruptions increase the risk of the company; disruptions have a significant negative impact on profitability; disruptions have a crippling effect on performance because firms take longer to recover from disruptions; and disruptions have a negative impact on stock price, profitability, and share price volatility across the board. The study recommends diversification of alcohol supply chains to mitigate the impact of disruptions, establish robust monitoring and surveillance systems to track changes in alcohol consumption patterns following supply chain disruptions and consider implementing price regulation and taxation policies to moderate alcohol consumption, particularly during periods of supply chain disruptions. , Thesis (MBA) -- Faculty of Business and Economic Sciences, Business School, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Sipamla, Noluthando
- Date: 2024-12
- Subjects: Business logistics , Inventory control , Alcoholism
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/69913 , vital:78172
- Description: This study investigated the effect of supply chain disruptions on alcohol consumer consumption behaviour in the Eastern Cape Province, but with the specific reference to Mdantsane Township in the Buffalo City Municipality. A quantitative methodology was employed, and self-administered questionnaires were used to collect data. The results of the study revealed that: disruptions increase the risk of the company; disruptions have a significant negative impact on profitability; disruptions have a crippling effect on performance because firms take longer to recover from disruptions; and disruptions have a negative impact on stock price, profitability, and share price volatility across the board. The study recommends diversification of alcohol supply chains to mitigate the impact of disruptions, establish robust monitoring and surveillance systems to track changes in alcohol consumption patterns following supply chain disruptions and consider implementing price regulation and taxation policies to moderate alcohol consumption, particularly during periods of supply chain disruptions. , Thesis (MBA) -- Faculty of Business and Economic Sciences, Business School, 2024
- Full Text:
- Date Issued: 2024-12
Comparative study of the effect of iloprost on neuroinflammatory changes in c8-b4 microglial cells and murine model of trypanosomiasis
- Authors: Jacobs, Ashleigh
- Date: 2024-12
- Subjects: Neuroimmunology , DNA -- Methylation , Nervous system -- Degeneration
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68895 , vital:77160
- Description: Neurodegenerative conditions significantly impact well-being and quality of life in individuals with major symptoms including mood disorders, cognitive decline, and psychiatric disturbances, often resulting from neuroinflammation triggered by immune responses to bacterial or parasitic infections such as gram-negative bacteria or Human African Trypanosomiasis. Microglia play a crucial role in both neurotoxicity and cellular processes involved in restoring the neural health. Exploring the therapeutic potential of prostacyclin and its analogues in regulating microglia responses to inflammatory insult and treating Trypanosoma brucei (T.b) infection remains an unexplored area. The aim of this study was to assess the potential neuroprotective effects of Iloprost through comparative analysis of neuroinflammatory responses in both microglial cells exposed to lipopolysaccharide (LPS) and mouse brains infected with T.b brucei. In phase I of this study both resting and LPS treated C8-B4 microglial cells were exposed to varying concentrations of Iloprost. The effects of Iloprost on LPS-induced inflammation were analysed using immunofluorescence to detect microglial activation and differentiate between pro and anti-inflammatory phenotypes. Furthermore, pro and anti-inflammatory cytokine secretion was determined using an ELISA, in addition gene expression analysis was carried out using quantitative polymerase chain reaction (qPCR). Also, DNA methylation status of C8-B4 cells exposed to LPS challenge alone or in combination with various concentrations of Iloprost were determined using bisulfite sequencing technique followed by qPCR. In phase II of the study, a total of twenty-four Albino Swiss male mice (8-10 weeks old) were divided into four treatment groups with 6 mice in each group. All treatment groups except the non-infected control were inoculated with the T.b brucei parasite. One group received a single intraperitoneal injection of Diminazene aceturate (4 mg kg-1)while the remaining group received repeated intraperitoneal injections of Iloprost (200 μg kg-1). On day ten of the study, mouse brains were removed on ice using forceps. The hippocampal tissues were dissected out and processed for quantification of gene expression changes in pro and anti-inflammatory cytokines. Overall, the findings of this study indicate that LPS-induced pro-inflammatory cytokine, TNF-α and IL-1β, secretion and gene expression is down-regulated in C8-B4 microglial cells treated with Iloprost. Furthermore, there was a significant up-regulation in the expression of anti-inflammatory genes, particularly ARG-1, CD206, BDNF and CREB in response to Iloprost treatment following LPS-induced inflammation. This study is also the first to confirm M2 microglial polarization with Iloprost treatment in both resting and LPS treated cells. However, hypermethylation at CREB and BDNF promoter regions was observed 24 hours after Iloprost treatment. Additionally, Iloprost reversed hypomethylation at the BDNF promoter region that had been induced by LPS treatment. The rodent model also indicated a downregulation in the pro-inflammatory cytokine, IL-1β, expression and upregulation of BDNF transcription in T.b brucei infected mice treated with repeated doses of Iloprost. In conclusion, determining the immunomodulatory roles of Iloprost in both in vitro and in vivo models of neuroinflammation could assist in the development of alternative therapy for neurodegenerative disease. , Thesis (MSc) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Jacobs, Ashleigh
- Date: 2024-12
- Subjects: Neuroimmunology , DNA -- Methylation , Nervous system -- Degeneration
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68895 , vital:77160
- Description: Neurodegenerative conditions significantly impact well-being and quality of life in individuals with major symptoms including mood disorders, cognitive decline, and psychiatric disturbances, often resulting from neuroinflammation triggered by immune responses to bacterial or parasitic infections such as gram-negative bacteria or Human African Trypanosomiasis. Microglia play a crucial role in both neurotoxicity and cellular processes involved in restoring the neural health. Exploring the therapeutic potential of prostacyclin and its analogues in regulating microglia responses to inflammatory insult and treating Trypanosoma brucei (T.b) infection remains an unexplored area. The aim of this study was to assess the potential neuroprotective effects of Iloprost through comparative analysis of neuroinflammatory responses in both microglial cells exposed to lipopolysaccharide (LPS) and mouse brains infected with T.b brucei. In phase I of this study both resting and LPS treated C8-B4 microglial cells were exposed to varying concentrations of Iloprost. The effects of Iloprost on LPS-induced inflammation were analysed using immunofluorescence to detect microglial activation and differentiate between pro and anti-inflammatory phenotypes. Furthermore, pro and anti-inflammatory cytokine secretion was determined using an ELISA, in addition gene expression analysis was carried out using quantitative polymerase chain reaction (qPCR). Also, DNA methylation status of C8-B4 cells exposed to LPS challenge alone or in combination with various concentrations of Iloprost were determined using bisulfite sequencing technique followed by qPCR. In phase II of the study, a total of twenty-four Albino Swiss male mice (8-10 weeks old) were divided into four treatment groups with 6 mice in each group. All treatment groups except the non-infected control were inoculated with the T.b brucei parasite. One group received a single intraperitoneal injection of Diminazene aceturate (4 mg kg-1)while the remaining group received repeated intraperitoneal injections of Iloprost (200 μg kg-1). On day ten of the study, mouse brains were removed on ice using forceps. The hippocampal tissues were dissected out and processed for quantification of gene expression changes in pro and anti-inflammatory cytokines. Overall, the findings of this study indicate that LPS-induced pro-inflammatory cytokine, TNF-α and IL-1β, secretion and gene expression is down-regulated in C8-B4 microglial cells treated with Iloprost. Furthermore, there was a significant up-regulation in the expression of anti-inflammatory genes, particularly ARG-1, CD206, BDNF and CREB in response to Iloprost treatment following LPS-induced inflammation. This study is also the first to confirm M2 microglial polarization with Iloprost treatment in both resting and LPS treated cells. However, hypermethylation at CREB and BDNF promoter regions was observed 24 hours after Iloprost treatment. Additionally, Iloprost reversed hypomethylation at the BDNF promoter region that had been induced by LPS treatment. The rodent model also indicated a downregulation in the pro-inflammatory cytokine, IL-1β, expression and upregulation of BDNF transcription in T.b brucei infected mice treated with repeated doses of Iloprost. In conclusion, determining the immunomodulatory roles of Iloprost in both in vitro and in vivo models of neuroinflammation could assist in the development of alternative therapy for neurodegenerative disease. , Thesis (MSc) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2024
- Full Text:
- Date Issued: 2024-12
Criteria for effective short-term rental business venture property selection in Nelson Mandela Bay
- Authors: Theunissen, Melanie
- Date: 2024-12
- Subjects: Real estate investment -- Rental property -- South Africa , Property investment -- Real estate business -- South Africa , Small business -- Management
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/69890 , vital:78143
- Description: Short-term rental (STR) accommodations thrive in the online sharing economy and are influenced by market trends and regulations and vary in location. Success hinges on careful planning, continuous management and fulfilling guest expectations. There is limited research on the types of properties that succeed in the STR business. Additionally, STR’s are regulated, competitive and entail financial planning which can offer both lucrative income potential and high expenses. This treatise is an effort to evaluate the criteria for property selection for STR’s as a business in Nelson Mandela Bay. The research approach for this study is positivistic. A literature review has been conducted to determine the existing status of STR studies relating to the business industry. The literature review allowed the formation of criteria which was tested with quantitative methods consisting of data collection, a questionnaire and an analysis of the statistics. This assessment clearly supported STR as a viable business venture. Based on the research study, criteria were created to help STR owners select the right property for STR. The four main considerations in STR accommodation business in this criteria framework included location, type and size of the property, features and amenities and safety and security. The research confirmed that in the STR market, these variables are proposed as key focal point for business owners to focus on and to positively affect the types of property that are purchased to grow the STR business. As a result, create favourable bottom line returns. In agreement with the literature that is reviewed and the outcomes of this treatise, by effectively addressing the criteria, the business owners of STR’s will create a criteria model that is helpful to purchase the most suitable property for a STR business. Certain key findings from the research found it to be true that you can improve property selection decision making through the identification of relevant criteria that can assist STR business investors and managers. It is proposed that the criteria is examined to provide further advancement to the STR industry. The recommendations include further improving the criteria by providing additional information about the STR business behaviours connected to each variable and increasing the scope of the study to more than just one sample from mainly the Eastern Cape in South Africa. , Thesis (MBA) -- Faculty Business and Economic Sciences, Business School, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Theunissen, Melanie
- Date: 2024-12
- Subjects: Real estate investment -- Rental property -- South Africa , Property investment -- Real estate business -- South Africa , Small business -- Management
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/69890 , vital:78143
- Description: Short-term rental (STR) accommodations thrive in the online sharing economy and are influenced by market trends and regulations and vary in location. Success hinges on careful planning, continuous management and fulfilling guest expectations. There is limited research on the types of properties that succeed in the STR business. Additionally, STR’s are regulated, competitive and entail financial planning which can offer both lucrative income potential and high expenses. This treatise is an effort to evaluate the criteria for property selection for STR’s as a business in Nelson Mandela Bay. The research approach for this study is positivistic. A literature review has been conducted to determine the existing status of STR studies relating to the business industry. The literature review allowed the formation of criteria which was tested with quantitative methods consisting of data collection, a questionnaire and an analysis of the statistics. This assessment clearly supported STR as a viable business venture. Based on the research study, criteria were created to help STR owners select the right property for STR. The four main considerations in STR accommodation business in this criteria framework included location, type and size of the property, features and amenities and safety and security. The research confirmed that in the STR market, these variables are proposed as key focal point for business owners to focus on and to positively affect the types of property that are purchased to grow the STR business. As a result, create favourable bottom line returns. In agreement with the literature that is reviewed and the outcomes of this treatise, by effectively addressing the criteria, the business owners of STR’s will create a criteria model that is helpful to purchase the most suitable property for a STR business. Certain key findings from the research found it to be true that you can improve property selection decision making through the identification of relevant criteria that can assist STR business investors and managers. It is proposed that the criteria is examined to provide further advancement to the STR industry. The recommendations include further improving the criteria by providing additional information about the STR business behaviours connected to each variable and increasing the scope of the study to more than just one sample from mainly the Eastern Cape in South Africa. , Thesis (MBA) -- Faculty Business and Economic Sciences, Business School, 2024
- Full Text:
- Date Issued: 2024-12
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