What are the pertinent intersections in the lives of black women at Rhodes University?
- Authors: Gushman, Lutho Phinda
- Date: 2021-10-29
- Subjects: Women, Black South Africa Makhanda , Student movements South Africa Makhanda , Intersectionality (Sociology) , Pluralism , Matrix organization South Africa Makhanda , Women, Black Education (Higher) South Africa Makhanda , Social action South Africa Makhanda , Rhodes University
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190990 , vital:45047
- Description: After the 2016 #FeesMustFall protest(s), higher education institutions were dramatically altered with respect to their institutional cultures; the narratives of those who were historically side-lined and marginalised took centre stage. At Rhodes University social activism was constitutive of three components; a ‘revolt’ against the fee increment; a contestation of the rape culture; and a rejection of the colonial curriculum. These concerns, in their varied articulations, made up different social and academic realities that define(d) Rhodes University and affected how individuals experienced institutional culture. According to Ndlovu (2017) while these expressed acts (in the form of protests and institutional shutdowns) of resistance against the system of higher education subsided after the fees must fall campaign, these served to centre the narratives of the marginalised. Keeping with this thinking, the argument presented in this thesis explores the experiences of black women in higher education after the call towards coordinated resistance. Using qualitative data in the form of narrative interviews, the thesis documents how the participants continued their academic and social life post-resistance. This rupture of resistance created a complex matrix of individual subjectivity where participants engaged with traditional social academic norms in new spaces of resistance; a phenomenon that enlivened the intersectionality that came to define the higher education landscape of the country. This thesis explores the stories of the participant’s as they engage(d) with what is becoming a new institution—that is the University in South Africa, with a case-in-point being Rhodes University—and to understand the power relations and intersections that define their lived experiences. This study found that the reality of existing within the confines of power—with its fluidity—meant that black women operate both within spaces of privilege and oppression simultaneously. As such, and following Vivian May’s (2015) argument, this study concludes that black women are situated and simultaneously constrained by power. Thus spaces of resistance are constantly in flux and determined by their relations within power. , Thesis (MA) -- Faculty of Humanities, Politics and International Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Gushman, Lutho Phinda
- Date: 2021-10-29
- Subjects: Women, Black South Africa Makhanda , Student movements South Africa Makhanda , Intersectionality (Sociology) , Pluralism , Matrix organization South Africa Makhanda , Women, Black Education (Higher) South Africa Makhanda , Social action South Africa Makhanda , Rhodes University
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190990 , vital:45047
- Description: After the 2016 #FeesMustFall protest(s), higher education institutions were dramatically altered with respect to their institutional cultures; the narratives of those who were historically side-lined and marginalised took centre stage. At Rhodes University social activism was constitutive of three components; a ‘revolt’ against the fee increment; a contestation of the rape culture; and a rejection of the colonial curriculum. These concerns, in their varied articulations, made up different social and academic realities that define(d) Rhodes University and affected how individuals experienced institutional culture. According to Ndlovu (2017) while these expressed acts (in the form of protests and institutional shutdowns) of resistance against the system of higher education subsided after the fees must fall campaign, these served to centre the narratives of the marginalised. Keeping with this thinking, the argument presented in this thesis explores the experiences of black women in higher education after the call towards coordinated resistance. Using qualitative data in the form of narrative interviews, the thesis documents how the participants continued their academic and social life post-resistance. This rupture of resistance created a complex matrix of individual subjectivity where participants engaged with traditional social academic norms in new spaces of resistance; a phenomenon that enlivened the intersectionality that came to define the higher education landscape of the country. This thesis explores the stories of the participant’s as they engage(d) with what is becoming a new institution—that is the University in South Africa, with a case-in-point being Rhodes University—and to understand the power relations and intersections that define their lived experiences. This study found that the reality of existing within the confines of power—with its fluidity—meant that black women operate both within spaces of privilege and oppression simultaneously. As such, and following Vivian May’s (2015) argument, this study concludes that black women are situated and simultaneously constrained by power. Thus spaces of resistance are constantly in flux and determined by their relations within power. , Thesis (MA) -- Faculty of Humanities, Politics and International Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
Biton’s Lost Siege Engine: Experimental archaeology in Classical Studies
- Authors: Rademan, David John
- Date: 2021-10-29
- Subjects: Pergamum (Extinct city) , Dimensional analysis , Engineering History To 168 B.C. , Fluid mechanics History To 168 B.C. , Byzantine literature History and criticism , Siege warfare History To 168 B.C. , Biton of Pergamon , Isidorus of Abydos , Poliorcetics
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192330 , vital:45216
- Description: This thesis entails an examination of several problems inherent in placing a technical treatise by the Hellenistic Greek engineer, Biton of Pergamon, at a siege of 156-154BCE, with a view to galvanising the existing case of previous scholarship through a combined approach of literary, textual, geographical, and technical analysis. Particular focus is given to the following problems: technical errors in current translations of the treatise of Biton; technical considerations in scholars’ reproductions of a particular engine in the treatise; an assessment of the practical implications of the treatise in situ at the physical site of the ancient city of Pergamon in the second century BCE, as evidenced by archaeological findings and surveys; assessment of those implications by way of historical records of similar conflicts from the Hellenistic period; and suggesting a procedure of dimensional analysis for testing a hypothesis regarding the feasibility of the ancient engineer’s recommended engines as a stand-in for the city’s original defenses, in a manner that harmonises the methodologies of historicism and experimental archaeology with sound and appropriate modern engineering practice from the field of Fluid Mechanics. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Rademan, David John
- Date: 2021-10-29
- Subjects: Pergamum (Extinct city) , Dimensional analysis , Engineering History To 168 B.C. , Fluid mechanics History To 168 B.C. , Byzantine literature History and criticism , Siege warfare History To 168 B.C. , Biton of Pergamon , Isidorus of Abydos , Poliorcetics
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192330 , vital:45216
- Description: This thesis entails an examination of several problems inherent in placing a technical treatise by the Hellenistic Greek engineer, Biton of Pergamon, at a siege of 156-154BCE, with a view to galvanising the existing case of previous scholarship through a combined approach of literary, textual, geographical, and technical analysis. Particular focus is given to the following problems: technical errors in current translations of the treatise of Biton; technical considerations in scholars’ reproductions of a particular engine in the treatise; an assessment of the practical implications of the treatise in situ at the physical site of the ancient city of Pergamon in the second century BCE, as evidenced by archaeological findings and surveys; assessment of those implications by way of historical records of similar conflicts from the Hellenistic period; and suggesting a procedure of dimensional analysis for testing a hypothesis regarding the feasibility of the ancient engineer’s recommended engines as a stand-in for the city’s original defenses, in a manner that harmonises the methodologies of historicism and experimental archaeology with sound and appropriate modern engineering practice from the field of Fluid Mechanics. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2021
- Full Text:
- Date Issued: 2021-10-29
An ethnographic investigation of the implementation of the bilingual-bicultural approach for educating deaf learners focusing on South African sign language teaching at FET level
- Authors: Tunzelana, Nomava Mercy
- Date: 2021-10-29
- Subjects: South African Sign Language Study and teaching (Higher) , Sign language acquisition South Africa , Education, Bilingual South Africa , Biculturalism South Africa , Ethnology South Africa , Culturally relevant pedagogy South Africa , Deaf students South Africa , South Africa. Department of Basic Education , Curriculum-based assessment South Africa , South African Curriculum Assessment and Policy Statement (CAPS)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192353 , vital:45218
- Description: This half-thesis reports on an ethnographic investigation of the implementation of the Bilingual-Bicultural Approach for educating Deaf learners, focusing on South African Sign Language (SASL) teaching at the Further Education and Training (FET) level in a South African School for the Deaf in the Eastern Cape. The investigation occurs within the context of the introduction, in 2015, of the SASL Curriculum and Assessment Policy Statement (CAPS) by the Department of Basic Education (DBE). The SASL CAPS introduces an approach to teaching the Deaf known as the Bilingual-Bicultural Approach. This is an approach in which natural sign language (such as SASL) is taught first and used to learn a spoken language such as English for reading and writing. Previous research on a contrastive analysis of South African English and SASL reveals that SASL is a Topic-Comment language. It is sometimes Object-Subject-Verb (OSV) or Subject-Object-Verb (SOV) while the word order of South African English is Subject-Verb-Object (SVO). Semi-structured interviews of hearing and Deaf participants reveal serious tensions between the staff and the hearing staff because Deaf culture is not adhered to by some of the hearing staff. These tensions have a negative impact on the culture of learning and teaching at the school. Observations of four lessons at an FET class taught by an SASL FET teacher show that in her teaching, SASL syntax is used in keeping with the principles of the Bilingual-Bicultural Approach. However, her signing is accompanied by unvoiced spoken language due to the influence of a previously used approach called Total Communication (TC). One of the learners, Lulu, who contributes considerably more often than other learners in the lesson, also shows the same influence of TC. Other learners are either withdrawn or copy signs from Lulu. The study concludes with the recommendation that SASL be used for initiating newcomers to school as opposed to Signed English because research in bilingualism suggests that second language learners need one natural language established first before attempting to learn a second language. Teachers are recommended to immerse themselves into Deaf culture to acquire fluency. Comments from some participants suggest that teachers require vigorous training in the Bilingual-Bicultural Approach on a continuous basis. , Thesis (MA) -- Faculty of Humanities, Linguistics and Applied Languages Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Tunzelana, Nomava Mercy
- Date: 2021-10-29
- Subjects: South African Sign Language Study and teaching (Higher) , Sign language acquisition South Africa , Education, Bilingual South Africa , Biculturalism South Africa , Ethnology South Africa , Culturally relevant pedagogy South Africa , Deaf students South Africa , South Africa. Department of Basic Education , Curriculum-based assessment South Africa , South African Curriculum Assessment and Policy Statement (CAPS)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192353 , vital:45218
- Description: This half-thesis reports on an ethnographic investigation of the implementation of the Bilingual-Bicultural Approach for educating Deaf learners, focusing on South African Sign Language (SASL) teaching at the Further Education and Training (FET) level in a South African School for the Deaf in the Eastern Cape. The investigation occurs within the context of the introduction, in 2015, of the SASL Curriculum and Assessment Policy Statement (CAPS) by the Department of Basic Education (DBE). The SASL CAPS introduces an approach to teaching the Deaf known as the Bilingual-Bicultural Approach. This is an approach in which natural sign language (such as SASL) is taught first and used to learn a spoken language such as English for reading and writing. Previous research on a contrastive analysis of South African English and SASL reveals that SASL is a Topic-Comment language. It is sometimes Object-Subject-Verb (OSV) or Subject-Object-Verb (SOV) while the word order of South African English is Subject-Verb-Object (SVO). Semi-structured interviews of hearing and Deaf participants reveal serious tensions between the staff and the hearing staff because Deaf culture is not adhered to by some of the hearing staff. These tensions have a negative impact on the culture of learning and teaching at the school. Observations of four lessons at an FET class taught by an SASL FET teacher show that in her teaching, SASL syntax is used in keeping with the principles of the Bilingual-Bicultural Approach. However, her signing is accompanied by unvoiced spoken language due to the influence of a previously used approach called Total Communication (TC). One of the learners, Lulu, who contributes considerably more often than other learners in the lesson, also shows the same influence of TC. Other learners are either withdrawn or copy signs from Lulu. The study concludes with the recommendation that SASL be used for initiating newcomers to school as opposed to Signed English because research in bilingualism suggests that second language learners need one natural language established first before attempting to learn a second language. Teachers are recommended to immerse themselves into Deaf culture to acquire fluency. Comments from some participants suggest that teachers require vigorous training in the Bilingual-Bicultural Approach on a continuous basis. , Thesis (MA) -- Faculty of Humanities, Linguistics and Applied Languages Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
Media consumption and identity formation: the consumption of Latin American telenovelas in two neighbourhoods of Maputo in Mozambique
- Authors: Ofumane, Alvo Naftal
- Date: 2021-10-29
- Subjects: Television soap operas Mozambique Maputo , Identity (Psychology) and mass media Mozambique Maputo , Television soap operas History and criticism , Visual reception theory Mozambique Maputo , Mozambique Politics and government , Mozambique Social conditions , Mozambique Social life and customs
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192287 , vital:45212
- Description: This study explores how the residents of two neighbourhoods in Maputo, KaMaxakeni and KaMpfumo interact with and make meanings of (Latin American) telenovelas, as part of their identity formation process. It is guided by questions on how the residents of these two neighbourhoods interact with telenovelas in their daily lives; how this shapes their own values and understanding of themselves and the world in which they live; what aspects of telenovelas they value, and what meanings they make from them; and, finally, what determines their choices of media consumption. The study adopted reception theory, rooted in qualitative methodology. This approach explores the ‘insider’s’ perspective of the research subjects, taking the actors’ perspective as the empirical point of departure. Using a purposive theoretical sampling procedure, targeting those families who are avid viewers of telenovelas, the data were generated through participant observation, focus groups discussions, and individual interviews. To understand and interpret the interface between the telenovela (medium) and its viewers (audience) in Maputo, the study used qualitative thematic content analysis of the telenovelas viewing process by the residents of KaMpfumo and KaMaxakeni. The data show that the residents of KaMpfumo and KaMaxakeni in Maputo interact and make sense of the telenovelas in various ways. Telenovelas are used as an educational tool; they are used to reinforce daily life world practices; they s shape people’s personal character; they become another family presence; they are used to build or strengthen physical and virtual viewing networks, and, “Pure” telenovela viewing is a relatively rare occurrence. , Thesis (MA) -- Faculty of Humanities, Journalism and Media Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Ofumane, Alvo Naftal
- Date: 2021-10-29
- Subjects: Television soap operas Mozambique Maputo , Identity (Psychology) and mass media Mozambique Maputo , Television soap operas History and criticism , Visual reception theory Mozambique Maputo , Mozambique Politics and government , Mozambique Social conditions , Mozambique Social life and customs
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192287 , vital:45212
- Description: This study explores how the residents of two neighbourhoods in Maputo, KaMaxakeni and KaMpfumo interact with and make meanings of (Latin American) telenovelas, as part of their identity formation process. It is guided by questions on how the residents of these two neighbourhoods interact with telenovelas in their daily lives; how this shapes their own values and understanding of themselves and the world in which they live; what aspects of telenovelas they value, and what meanings they make from them; and, finally, what determines their choices of media consumption. The study adopted reception theory, rooted in qualitative methodology. This approach explores the ‘insider’s’ perspective of the research subjects, taking the actors’ perspective as the empirical point of departure. Using a purposive theoretical sampling procedure, targeting those families who are avid viewers of telenovelas, the data were generated through participant observation, focus groups discussions, and individual interviews. To understand and interpret the interface between the telenovela (medium) and its viewers (audience) in Maputo, the study used qualitative thematic content analysis of the telenovelas viewing process by the residents of KaMpfumo and KaMaxakeni. The data show that the residents of KaMpfumo and KaMaxakeni in Maputo interact and make sense of the telenovelas in various ways. Telenovelas are used as an educational tool; they are used to reinforce daily life world practices; they s shape people’s personal character; they become another family presence; they are used to build or strengthen physical and virtual viewing networks, and, “Pure” telenovela viewing is a relatively rare occurrence. , Thesis (MA) -- Faculty of Humanities, Journalism and Media Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
Tense and aspect in Xhosa
- Authors: Savić, Stefan
- Date: 2021-10-29
- Subjects: Xhosa language , Xhosa language Grammar , Xhosa language Semantics , Xhosa language Tense , Xhosa language Aspect , Xhosa language Syntax , Xhosa language Morphology , Xhosa language Grammar, Comparative , Information structure
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/192897 , vital:45277 , 10.21504/10962/192897
- Description: This dissertation investigates the semantics of each tense and aspect in Xhosa. Since tense and aspect perform important pragmatic functions, the analysis takes into account the correlation between the verb and the wider discourse in which it is embedded. Tense reflects the temporal relation between the time of the utterance (speech time) and an interval the speaker makes the assertion about (reference time). The Remote Past and the Remote Future tenses differ from their Recent/Immediate counterparts in that they denote events which occurred in a significantly different situation than the speech time and/or events in the surrounding discourse. Aspect does not only indicate the relation between the time occupied by the real world event and the reference time chosen by the speaker. The Perfective aspect represents an event as a unique change-of-state that pertains to a single point on the timeline which at the same time functions as the reference time. By contrast, for the Imperfective aspect temporally links the event to a contextually provided reference time, e.g. the utterance time, a time adverbial, a period of time previously introduced in the preceding discourse, or the interlocutors’ shared experience. At the pragmatic level, the Perfective aspect tends to introduce an event’s resulting state into the discourse, whereas the Imperfective aspect tends to rule it out. Like the Imperfective aspect, the Anterior and the Prospective aspects assert an event’s occurrence from a contextually defined reference time. They refer to the consequent and the preparatory states of an event, respectively. On the pragmatic level, the Anterior aspect may also indicate that the truth-conditionality of the event’s resulting state is contradicted in the immediate discourse. This study shows that tense and aspect temporally represent different means of temporally assigning an event to a particular portion of the timeline. I further argue that aspect indicates whether the reference time is provided in the context (Imperfective, Anterior, Prospective) or whether it is introduced by the verb itself (Perfective). Furthermore, this study shows that aspect exhibits a pragmatic function by laying focus on different parts of the event that are relevant in the upcoming discourse. , Thesis (PhD) -- Faculty of Humanities, Linguistics and Applied Languages Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Savić, Stefan
- Date: 2021-10-29
- Subjects: Xhosa language , Xhosa language Grammar , Xhosa language Semantics , Xhosa language Tense , Xhosa language Aspect , Xhosa language Syntax , Xhosa language Morphology , Xhosa language Grammar, Comparative , Information structure
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/192897 , vital:45277 , 10.21504/10962/192897
- Description: This dissertation investigates the semantics of each tense and aspect in Xhosa. Since tense and aspect perform important pragmatic functions, the analysis takes into account the correlation between the verb and the wider discourse in which it is embedded. Tense reflects the temporal relation between the time of the utterance (speech time) and an interval the speaker makes the assertion about (reference time). The Remote Past and the Remote Future tenses differ from their Recent/Immediate counterparts in that they denote events which occurred in a significantly different situation than the speech time and/or events in the surrounding discourse. Aspect does not only indicate the relation between the time occupied by the real world event and the reference time chosen by the speaker. The Perfective aspect represents an event as a unique change-of-state that pertains to a single point on the timeline which at the same time functions as the reference time. By contrast, for the Imperfective aspect temporally links the event to a contextually provided reference time, e.g. the utterance time, a time adverbial, a period of time previously introduced in the preceding discourse, or the interlocutors’ shared experience. At the pragmatic level, the Perfective aspect tends to introduce an event’s resulting state into the discourse, whereas the Imperfective aspect tends to rule it out. Like the Imperfective aspect, the Anterior and the Prospective aspects assert an event’s occurrence from a contextually defined reference time. They refer to the consequent and the preparatory states of an event, respectively. On the pragmatic level, the Anterior aspect may also indicate that the truth-conditionality of the event’s resulting state is contradicted in the immediate discourse. This study shows that tense and aspect temporally represent different means of temporally assigning an event to a particular portion of the timeline. I further argue that aspect indicates whether the reference time is provided in the context (Imperfective, Anterior, Prospective) or whether it is introduced by the verb itself (Perfective). Furthermore, this study shows that aspect exhibits a pragmatic function by laying focus on different parts of the event that are relevant in the upcoming discourse. , Thesis (PhD) -- Faculty of Humanities, Linguistics and Applied Languages Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
Exploring the political economy of the broadcast and distribution of the Mzansi Super League in South Africa
- Authors: Pettitt, Stephen John
- Date: 2021-10-29
- Subjects: Cricket Political aspects South Africa , Discrimination in sports South Africa , South Africa Social conditions , South Africa Economic conditions , Broadcasting South Africa , Cricket South Africa (Organization) , Television broadcasting of sports , Free-to-air broadcasting (FTA) South Africa , Mzansi Super League
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192308 , vital:45215
- Description: This thesis is a critical political economy analysis of the role played by the broadcast and distribution of the Mzansi Super League (MSL), a T20 cricket tournament in South Africa, in the transformation project of Cricket South Africa (CSA). The case study methodology is based on the four-leaf clover framework developed by Ruth Teer-Tomaselli. Cricket in South Africa is often viewed as a white and middle-class dominated sport, and CSA has been mandated to redress the historical racially segregated sport through a transformation agenda that focuses on changing the demographics of cricket. This paper investigates how the broadcast of a cricket tournament like the MSL on a Public Service Broadcaster (PSB) can have a positive effect on transformation due to the increased access and wider distribution. The broadcast regulations acknowledge this, as is seen in the introduction of ICASA draft regulations seeking to make more sport available on PSBs. The South African sports broadcast landscape is dominated by MultiChoice’s SuperSport, which has acquired a monopoly on sports broadcasting. The South African Broadcast Corporation (SABC) cannot compete, given its financial difficulties. A fall-out between CSA and SuperSport meant the SABC broadcast the MSL tournament, which lost CSA revenue but gained it a bigger audience. This thesis uses lived experiences and examples to illustrate that despite a financial loss, the wider media distribution of cricket will have positive effects on transformation that could counter the financial loss, especially if CSA creates new streams of income so that it relies less on broadcast revenue. In the current climate, however, compounded by internal financial and management issues, CSA cannot afford to forego the revenue from a private broadcaster and thus has no choice but to pursue a deal with a private broadcaster and lose out on the potential benefits of a wider audience. , Thesis (MA) -- Faculty of Humanities, Journalism and Media Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Pettitt, Stephen John
- Date: 2021-10-29
- Subjects: Cricket Political aspects South Africa , Discrimination in sports South Africa , South Africa Social conditions , South Africa Economic conditions , Broadcasting South Africa , Cricket South Africa (Organization) , Television broadcasting of sports , Free-to-air broadcasting (FTA) South Africa , Mzansi Super League
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192308 , vital:45215
- Description: This thesis is a critical political economy analysis of the role played by the broadcast and distribution of the Mzansi Super League (MSL), a T20 cricket tournament in South Africa, in the transformation project of Cricket South Africa (CSA). The case study methodology is based on the four-leaf clover framework developed by Ruth Teer-Tomaselli. Cricket in South Africa is often viewed as a white and middle-class dominated sport, and CSA has been mandated to redress the historical racially segregated sport through a transformation agenda that focuses on changing the demographics of cricket. This paper investigates how the broadcast of a cricket tournament like the MSL on a Public Service Broadcaster (PSB) can have a positive effect on transformation due to the increased access and wider distribution. The broadcast regulations acknowledge this, as is seen in the introduction of ICASA draft regulations seeking to make more sport available on PSBs. The South African sports broadcast landscape is dominated by MultiChoice’s SuperSport, which has acquired a monopoly on sports broadcasting. The South African Broadcast Corporation (SABC) cannot compete, given its financial difficulties. A fall-out between CSA and SuperSport meant the SABC broadcast the MSL tournament, which lost CSA revenue but gained it a bigger audience. This thesis uses lived experiences and examples to illustrate that despite a financial loss, the wider media distribution of cricket will have positive effects on transformation that could counter the financial loss, especially if CSA creates new streams of income so that it relies less on broadcast revenue. In the current climate, however, compounded by internal financial and management issues, CSA cannot afford to forego the revenue from a private broadcaster and thus has no choice but to pursue a deal with a private broadcaster and lose out on the potential benefits of a wider audience. , Thesis (MA) -- Faculty of Humanities, Journalism and Media Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
In silico identification of natural inhibitory compounds against the Mycobacterium tuberculosis Enzyme Pyrazinamidase using high-throughput virtual screening techniques
- Authors: Kenyon, Thomas
- Date: 2021-10-29
- Subjects: Mycobacterium tuberculosis , Pyrazinamide , Molecular dynamics , High throughput screening (Drug development) , Mutagenesis , South African Natural Compounds database (SANCDB)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192074 , vital:45193
- Description: Tuberculosis (TB) is most commonly a pulmonary infection caused by the bacterium Mycobacterium tuberculosis. With the exception of the COVID-19 pandemic, TB was the most common cause of death due to an infectious disease for a number of years up until 2020. In 2019, 10 million people fell ill with TB worldwide and 1.4 million people died (WHO, 2020a). Additionally, multidrug-resistant TB (MDR-TB) remains a public health crisis and a health security threat. A global total of 206 030 people with multidrug- or rifampicin-resistant TB (MDR/RR-TB) were reported in 2019, a 10% increase from 186 883 in 2018. South Africa is ranked among the 48 high TB burden countries, with an estimated 360 000 people falling ill in 2019, resulting in 58 000 deaths, the majority of which being among people living with HIV. Unlike HIV, however, TB is a curable disease when managed correctly with long durations of antitubercular chemotherapy. Pyrazinamide (PZA) is an important first-line tuberculosis drug unique for its activity against latent TB. PZA is a prodrug, being converted into its active form, pyrazinoic acid (POA) by the Mtb gene pncA, coding for the pyrazinamidase enzyme (PZase). TB resistance to first-line drugs such as PZA is commonly associated with mutations in the pncA/PZase enzyme. This study aimed to identify potential novel inhibitors that bind to the active site of PZase. By making use of molecular docking studies and molecular dynamics (MD) simulations, high throughput virtual screening was performed on 623 compounds from the South African Natural Compounds database (SANCDB; https://sancdb.rubi.ru.ac.za). Ligands that selectively bound to the PZase active site were identified using docking studies, followed by MD simulations to assess ligand-PZase complex stability, Finally, hit compounds identified from the first round of MD simulations were screened again against PZase structures with high confidence point mutations known to infer PZA resistance in order to identify any novel compounds which had inhibitory potential against both WT and mutant forms of the PZase enzyme. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Kenyon, Thomas
- Date: 2021-10-29
- Subjects: Mycobacterium tuberculosis , Pyrazinamide , Molecular dynamics , High throughput screening (Drug development) , Mutagenesis , South African Natural Compounds database (SANCDB)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192074 , vital:45193
- Description: Tuberculosis (TB) is most commonly a pulmonary infection caused by the bacterium Mycobacterium tuberculosis. With the exception of the COVID-19 pandemic, TB was the most common cause of death due to an infectious disease for a number of years up until 2020. In 2019, 10 million people fell ill with TB worldwide and 1.4 million people died (WHO, 2020a). Additionally, multidrug-resistant TB (MDR-TB) remains a public health crisis and a health security threat. A global total of 206 030 people with multidrug- or rifampicin-resistant TB (MDR/RR-TB) were reported in 2019, a 10% increase from 186 883 in 2018. South Africa is ranked among the 48 high TB burden countries, with an estimated 360 000 people falling ill in 2019, resulting in 58 000 deaths, the majority of which being among people living with HIV. Unlike HIV, however, TB is a curable disease when managed correctly with long durations of antitubercular chemotherapy. Pyrazinamide (PZA) is an important first-line tuberculosis drug unique for its activity against latent TB. PZA is a prodrug, being converted into its active form, pyrazinoic acid (POA) by the Mtb gene pncA, coding for the pyrazinamidase enzyme (PZase). TB resistance to first-line drugs such as PZA is commonly associated with mutations in the pncA/PZase enzyme. This study aimed to identify potential novel inhibitors that bind to the active site of PZase. By making use of molecular docking studies and molecular dynamics (MD) simulations, high throughput virtual screening was performed on 623 compounds from the South African Natural Compounds database (SANCDB; https://sancdb.rubi.ru.ac.za). Ligands that selectively bound to the PZase active site were identified using docking studies, followed by MD simulations to assess ligand-PZase complex stability, Finally, hit compounds identified from the first round of MD simulations were screened again against PZase structures with high confidence point mutations known to infer PZA resistance in order to identify any novel compounds which had inhibitory potential against both WT and mutant forms of the PZase enzyme. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Date Issued: 2021-10-29
The de novo biosynthesis of biotin is required for the optimal growth of Salmonella enterica serovar Typhimurium in the intracellular environment
- Authors: McLaughlin, Claire
- Date: 2021-10-29
- Subjects: Salmonella , Biotin , Biosynthesis , Salmonella typhimurium , Antibacterial agents , Anti-infective agents , Pathogenic bacteria , Salmonella food poisoning
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192097 , vital:45195
- Description: Salmonella enterica serovar Typhimurium (S. Typhimurium) is a foodborne pathogen infecting humans and animals, contributing to significant morbidity and mortality worldwide each year. The increase in antibiotic-resistant S. Typhimurium infections in recent years has highlighted the need for new antibacterial drugs and drug targets. S. Typhimurium can acquire biotin through de novo biosynthesis or via transport from its extracellular environment. The importance of the vitamin for bacterial survival, coupled with the absence of the biotin biosynthetic pathway in humans, makes the biotin biosynthetic enzymes attractive targets for drug discovery. The study's primary aim was to determine the relative importance of the biotin biosynthesis and transport pathways for the in vitro and ex vivo growth and survival of S. Typhimurium, with the goal of validating the pathways as valid targets for antimicrobial drug development. In order to achieve this aim, we generated S. Typhimurium mutant strains harbouring deletions in either the biotin biosynthetic gene, bioB, or putative high-affinity biotin transporter, yigM (ΔbioB and ΔyigM, respectively), as well as a double mutant in which the two mutations were combined (ΔbioB ΔyigM). Since the simultaneous disruption of biotin biosynthesis and transport in the double mutant may form a synthetic lethal combination, preventing further analysis of the strain, we also constructed a conditional mutant in which the promoter of the yigM gene was replaced by the arabinose-regulatable, PBAD promoter in the ΔbioB background (ΔbioB PBAD::yigM). Since the expression of the YigM in this strain is arabinose-regulatable, its role as a biotin transporter can be evaluated by altering the arabinose concentration in the growth media. Once the mutant strains were isolated and verified genetically, their growth and that of their genetically complemented counterparts were analysed in liquid and/or solid M9 minimal medium in the absence of biotin. Consistent with previous observations, the ΔbioB auxotrophic mutant's growth was severely compromised in minimal media in the absence of biotin. The growth of the strain could, however, be restored by supplementation with exogenous biotin or expression of the wild type bioB gene from an episomal plasmid. The ability of biotin to reverse the growth defect of the ΔbioB mutant strain was, however, dependent on the presence of a functional YigM, since biotin supplementation did not affect the growth of the ΔbioB ΔyigM double mutant strain. The introduction of a second copy of the yigM gene in the ΔbioB ΔyigM background, however, restored the growth of the strain in the presence, but not absence, of biotin. The dependence of the double mutant on both YigM and biotin for growth supports the idea that the protein functions as the sole or primary biotin transporter in S. Typhimurium, as it has recently been shown for E. coli (Ringsletter, 2010; Finkenwirth et al, 2013). The essentiality of YigM for biotin transport was subsequently verified by two independent means. Firstly, the growth of the ΔbioB PBAD::yigM promoter-replacement mutant was strictly dependent on the inclusion of arabinose in biotin-supplemented M9 minimal media supplemented, indicating that the expression of YigM from the PBAD promoter is essential for biotin transport. Secondly, following treatment with a known small-molecule inhibitor of the biotin biosynthesis, MAC-13772, exogenous biotin was capable of restoring the growth defect of the YigM+ wild type S. Typhimurium strain, but not the YigM− ΔyigM mutant. Taken together, these findings confirm that YigM serves as the biotin transporter for S. Typhimurium and that the corresponding ΔyigM mutant is, as a result, defective for biotin transport. Having confirmed the genotypes and phenotypes of the ΔbioB, ΔyigM, and ΔbioB ΔyigM mutants, we next analysed the importance of the biotin biosynthesis and transport pathways for the growth and survival of S. Typhimurium within the intracellular environment. To this end, we determined the proliferation of each of the mutant strains following infection of HeLa epithelial and RAW264.7 macrophage-like cell lines. Our results revealed that the de novo biosynthesis of biotin is required for the optimal growth of S. Typhimurium following infection of both epithelial and macrophage-like cell lines. Disruption of biotin transport, by contrast, had no significant effect on the intracellular proliferation of S. Typhimurium when a functional pathway for the biosynthesis of biotin was present. The simultaneous disruption of biotin biosynthesis and transport, however, resulted in significant attenuation of S. Typhimurium in epithelial cells, while bacterial survival in macrophages decreased to below the limit of detection. Overall, our results suggest the S. Typhimurium relies primarily on biotin produced by the de novo biosynthesis pathway to support its growth in the intracellular environment. While YigM-mediated biotin transport is essential for sustaining the viability of intracellular S. Typhimurium in the absence of de novo biosynthesis, it appears to play a relatively minor role in the acquisition of biotin during growth in the nutrient-limited Salmonella containing vacuole. Our findings suggest that inhibiting biotin biosynthesis may be a viable strategy for combating systemic infections caused by Salmonella, as has been recently proposed for other medically important bacterial pathogens (Carfrae et al., 2020). , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: McLaughlin, Claire
- Date: 2021-10-29
- Subjects: Salmonella , Biotin , Biosynthesis , Salmonella typhimurium , Antibacterial agents , Anti-infective agents , Pathogenic bacteria , Salmonella food poisoning
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192097 , vital:45195
- Description: Salmonella enterica serovar Typhimurium (S. Typhimurium) is a foodborne pathogen infecting humans and animals, contributing to significant morbidity and mortality worldwide each year. The increase in antibiotic-resistant S. Typhimurium infections in recent years has highlighted the need for new antibacterial drugs and drug targets. S. Typhimurium can acquire biotin through de novo biosynthesis or via transport from its extracellular environment. The importance of the vitamin for bacterial survival, coupled with the absence of the biotin biosynthetic pathway in humans, makes the biotin biosynthetic enzymes attractive targets for drug discovery. The study's primary aim was to determine the relative importance of the biotin biosynthesis and transport pathways for the in vitro and ex vivo growth and survival of S. Typhimurium, with the goal of validating the pathways as valid targets for antimicrobial drug development. In order to achieve this aim, we generated S. Typhimurium mutant strains harbouring deletions in either the biotin biosynthetic gene, bioB, or putative high-affinity biotin transporter, yigM (ΔbioB and ΔyigM, respectively), as well as a double mutant in which the two mutations were combined (ΔbioB ΔyigM). Since the simultaneous disruption of biotin biosynthesis and transport in the double mutant may form a synthetic lethal combination, preventing further analysis of the strain, we also constructed a conditional mutant in which the promoter of the yigM gene was replaced by the arabinose-regulatable, PBAD promoter in the ΔbioB background (ΔbioB PBAD::yigM). Since the expression of the YigM in this strain is arabinose-regulatable, its role as a biotin transporter can be evaluated by altering the arabinose concentration in the growth media. Once the mutant strains were isolated and verified genetically, their growth and that of their genetically complemented counterparts were analysed in liquid and/or solid M9 minimal medium in the absence of biotin. Consistent with previous observations, the ΔbioB auxotrophic mutant's growth was severely compromised in minimal media in the absence of biotin. The growth of the strain could, however, be restored by supplementation with exogenous biotin or expression of the wild type bioB gene from an episomal plasmid. The ability of biotin to reverse the growth defect of the ΔbioB mutant strain was, however, dependent on the presence of a functional YigM, since biotin supplementation did not affect the growth of the ΔbioB ΔyigM double mutant strain. The introduction of a second copy of the yigM gene in the ΔbioB ΔyigM background, however, restored the growth of the strain in the presence, but not absence, of biotin. The dependence of the double mutant on both YigM and biotin for growth supports the idea that the protein functions as the sole or primary biotin transporter in S. Typhimurium, as it has recently been shown for E. coli (Ringsletter, 2010; Finkenwirth et al, 2013). The essentiality of YigM for biotin transport was subsequently verified by two independent means. Firstly, the growth of the ΔbioB PBAD::yigM promoter-replacement mutant was strictly dependent on the inclusion of arabinose in biotin-supplemented M9 minimal media supplemented, indicating that the expression of YigM from the PBAD promoter is essential for biotin transport. Secondly, following treatment with a known small-molecule inhibitor of the biotin biosynthesis, MAC-13772, exogenous biotin was capable of restoring the growth defect of the YigM+ wild type S. Typhimurium strain, but not the YigM− ΔyigM mutant. Taken together, these findings confirm that YigM serves as the biotin transporter for S. Typhimurium and that the corresponding ΔyigM mutant is, as a result, defective for biotin transport. Having confirmed the genotypes and phenotypes of the ΔbioB, ΔyigM, and ΔbioB ΔyigM mutants, we next analysed the importance of the biotin biosynthesis and transport pathways for the growth and survival of S. Typhimurium within the intracellular environment. To this end, we determined the proliferation of each of the mutant strains following infection of HeLa epithelial and RAW264.7 macrophage-like cell lines. Our results revealed that the de novo biosynthesis of biotin is required for the optimal growth of S. Typhimurium following infection of both epithelial and macrophage-like cell lines. Disruption of biotin transport, by contrast, had no significant effect on the intracellular proliferation of S. Typhimurium when a functional pathway for the biosynthesis of biotin was present. The simultaneous disruption of biotin biosynthesis and transport, however, resulted in significant attenuation of S. Typhimurium in epithelial cells, while bacterial survival in macrophages decreased to below the limit of detection. Overall, our results suggest the S. Typhimurium relies primarily on biotin produced by the de novo biosynthesis pathway to support its growth in the intracellular environment. While YigM-mediated biotin transport is essential for sustaining the viability of intracellular S. Typhimurium in the absence of de novo biosynthesis, it appears to play a relatively minor role in the acquisition of biotin during growth in the nutrient-limited Salmonella containing vacuole. Our findings suggest that inhibiting biotin biosynthesis may be a viable strategy for combating systemic infections caused by Salmonella, as has been recently proposed for other medically important bacterial pathogens (Carfrae et al., 2020). , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Date Issued: 2021-10-29
Curriculum adjustment and adaptive leadership in two service-learning courses at Rhodes University as a consequence of the COVID-19 pandemic
- Authors: Khuhlane, Heide Nozuko
- Date: 2021-10-29
- Subjects: COVID-19 (Disease) , Curriculum planning South Africa Makhanda , Rhodes University , Service learning South Africa Makhanda , Educational leadership South Africa Makhanda , Educational change South Africa Makhanda , Adaptive leadership
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191192 , vital:45069
- Description: The COVID-19 global pandemic altered many aspects of learning. Learning through service, a component of community engagement in higher education linking academic learning and the community was no exception. Informed by Experiential Learning Theory, this study investigated the curriculum adjustment of two service-learning courses at Rhodes University and the leadership development of those who lead the courses as a consequence of COVID-19. The study’s initial goal was to highlight the position of service-learning as a component of academic learning. With the onset of the COVID-19 pandemic the goal was extended to understanding the impact of the pandemic not only on service-learning, but on leadership as well. Furthermore, the study sought to determine the responsiveness of service-learning policies at Rhodes University at a time of crisis. The study was designed as an interpretivist case study with four participants and one secondary participant. The study employed document analysis, individual interviews and a focus group interview to collect data. Data analysis took the form of content analysis and coding, through the lens of Experiential Learning Theory and an alternative service-based model. The study findings revealed that as a result of the COVID-19 global pandemic both service-learning courses had to be adapted to ensure successful completion. The adaptations included attention to scaffolded learning, assessment and course outcomes; in one course the service engagement aspect with the community was lost entirely to ensure the saftey of students through adherence to COVID-19 safety regulations. The study also found that the participants developed adaptive leadership competencies and skills, technological and collaboration skills as well as a heightened regard for pastoral care and social justice. However, it was evident that the pandemic revealed gaps in the conceptual understanding of service-learning in the context of the two courses, a need for responsive policy, and practical strategies to implement those policies in smaller units in the institution. The study thus recommends an alternative service-based model approach to service-learning, increased policy responsiveness to issues posed by the ‘new normal’ to support adaptive leadership development, re-defining of the university-community partnership and the identification of opportunities for innovation and collaboration intra-departmentally through service-learning. , Thesis (MEd) -- Faculty of Education, Education, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Khuhlane, Heide Nozuko
- Date: 2021-10-29
- Subjects: COVID-19 (Disease) , Curriculum planning South Africa Makhanda , Rhodes University , Service learning South Africa Makhanda , Educational leadership South Africa Makhanda , Educational change South Africa Makhanda , Adaptive leadership
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191192 , vital:45069
- Description: The COVID-19 global pandemic altered many aspects of learning. Learning through service, a component of community engagement in higher education linking academic learning and the community was no exception. Informed by Experiential Learning Theory, this study investigated the curriculum adjustment of two service-learning courses at Rhodes University and the leadership development of those who lead the courses as a consequence of COVID-19. The study’s initial goal was to highlight the position of service-learning as a component of academic learning. With the onset of the COVID-19 pandemic the goal was extended to understanding the impact of the pandemic not only on service-learning, but on leadership as well. Furthermore, the study sought to determine the responsiveness of service-learning policies at Rhodes University at a time of crisis. The study was designed as an interpretivist case study with four participants and one secondary participant. The study employed document analysis, individual interviews and a focus group interview to collect data. Data analysis took the form of content analysis and coding, through the lens of Experiential Learning Theory and an alternative service-based model. The study findings revealed that as a result of the COVID-19 global pandemic both service-learning courses had to be adapted to ensure successful completion. The adaptations included attention to scaffolded learning, assessment and course outcomes; in one course the service engagement aspect with the community was lost entirely to ensure the saftey of students through adherence to COVID-19 safety regulations. The study also found that the participants developed adaptive leadership competencies and skills, technological and collaboration skills as well as a heightened regard for pastoral care and social justice. However, it was evident that the pandemic revealed gaps in the conceptual understanding of service-learning in the context of the two courses, a need for responsive policy, and practical strategies to implement those policies in smaller units in the institution. The study thus recommends an alternative service-based model approach to service-learning, increased policy responsiveness to issues posed by the ‘new normal’ to support adaptive leadership development, re-defining of the university-community partnership and the identification of opportunities for innovation and collaboration intra-departmentally through service-learning. , Thesis (MEd) -- Faculty of Education, Education, 2021
- Full Text:
- Date Issued: 2021-10-29
Climate change and small-scale farmer livelihood adaptation in rural border communities in Southern Africa: A case study of Ezondweni Village in Mchinji, Malawi
- Authors: Simango, Kennedy Khuzwayo
- Date: 2021-10-29
- Subjects: Farms, Small Malawi Mchinji , Climatic changes Economic aspects Malawi Mchinji , Climatic changes Risk management Malawi Mchinji , Crops and climate Malawi Mchinji , Sustainable agriculture Malawi Mchinji , Farmers Social networks Malawi Mchinji , Farm income Malawi Mchinji
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190008 , vital:44955
- Description: Southern Africa is a region increasingly experiencing the detrimental effects of climate change and variability. The rural areas in particular face unprecedented climate change-induced challenges in relation to small-scale farmers being able to pursue household-based livelihoods, and these farmers seek ways of adapting to climate change and variability in doing so. Some rural communities exist along territorial borders and their attempts at adapting to climate change entails cross-border networks and activities. In this context, this thesis examines small-scale farmer adaptation to climate change and variability in the small village of Ezondweni in Malawi near the Zambian border. Analytically, the study draws upon the Sustainable Livelihoods Framework as well as social network theory and the theory of reflexive agency put forward by Margaret Archer. A qualitative research design entailed informal interviews with a small number of Ezondweni villagers, focusing on their perceptions of climate change and their practices of climate change adaptation. The study demonstrates the diverse activities pursued by villagers, both within Malawi and beyond, in seeking to maintain household-based livelihoods, as well as the significance of mutually-supportive social networks which facilitate these livelihood processes. , Thesis (MSocSci) -- Faculty of Humanities, Social Science, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Simango, Kennedy Khuzwayo
- Date: 2021-10-29
- Subjects: Farms, Small Malawi Mchinji , Climatic changes Economic aspects Malawi Mchinji , Climatic changes Risk management Malawi Mchinji , Crops and climate Malawi Mchinji , Sustainable agriculture Malawi Mchinji , Farmers Social networks Malawi Mchinji , Farm income Malawi Mchinji
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190008 , vital:44955
- Description: Southern Africa is a region increasingly experiencing the detrimental effects of climate change and variability. The rural areas in particular face unprecedented climate change-induced challenges in relation to small-scale farmers being able to pursue household-based livelihoods, and these farmers seek ways of adapting to climate change and variability in doing so. Some rural communities exist along territorial borders and their attempts at adapting to climate change entails cross-border networks and activities. In this context, this thesis examines small-scale farmer adaptation to climate change and variability in the small village of Ezondweni in Malawi near the Zambian border. Analytically, the study draws upon the Sustainable Livelihoods Framework as well as social network theory and the theory of reflexive agency put forward by Margaret Archer. A qualitative research design entailed informal interviews with a small number of Ezondweni villagers, focusing on their perceptions of climate change and their practices of climate change adaptation. The study demonstrates the diverse activities pursued by villagers, both within Malawi and beyond, in seeking to maintain household-based livelihoods, as well as the significance of mutually-supportive social networks which facilitate these livelihood processes. , Thesis (MSocSci) -- Faculty of Humanities, Social Science, 2021
- Full Text:
- Date Issued: 2021-10-29
Pessimism and the secular problem of evil
- Authors: Ruiters, Siphamandla
- Date: 2021-10-29
- Subjects: Good and evil , Pessimism , Optimism , Secularism , Quality of life
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190111 , vital:44964
- Description: How should we respond to the prevalence of ubiquitous evil? In this thesis, I present two responses to the secular problem of evil. The secular problem of evil exists because we want to live good lives, but we are all existentially vulnerable to evil. Consequently, we can think of various ways in which evil's existence poses a problem for us. When faced with such evil, I distinguish two responses that we may adopt, namely, optimistic or pessimistic responses to the secular problem of evil. These responses may be understood as stances that we may adopt about the relative evil and goodness in the world. Once I have made a thorough evaluation of the discussion around the secular problem of evil and the responses, I will suggest that pessimism is an appealing and appropriate response that we should adopt. To achieve the aims of this thesis, I will first clarify the kind of evil at stake in this thesis and then explain how philosophers have historically responded to this problem. Secondly, I will focus on the contemporary discussion on the secular problem of evil and show the responses are optimistic responses. Finally, I will provide a case against optimism and then show why pessimism is the most appealing and appropriate response. , Thesis (MA) -- Faculty of Humanities, Philosophy, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Ruiters, Siphamandla
- Date: 2021-10-29
- Subjects: Good and evil , Pessimism , Optimism , Secularism , Quality of life
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190111 , vital:44964
- Description: How should we respond to the prevalence of ubiquitous evil? In this thesis, I present two responses to the secular problem of evil. The secular problem of evil exists because we want to live good lives, but we are all existentially vulnerable to evil. Consequently, we can think of various ways in which evil's existence poses a problem for us. When faced with such evil, I distinguish two responses that we may adopt, namely, optimistic or pessimistic responses to the secular problem of evil. These responses may be understood as stances that we may adopt about the relative evil and goodness in the world. Once I have made a thorough evaluation of the discussion around the secular problem of evil and the responses, I will suggest that pessimism is an appealing and appropriate response that we should adopt. To achieve the aims of this thesis, I will first clarify the kind of evil at stake in this thesis and then explain how philosophers have historically responded to this problem. Secondly, I will focus on the contemporary discussion on the secular problem of evil and show the responses are optimistic responses. Finally, I will provide a case against optimism and then show why pessimism is the most appealing and appropriate response. , Thesis (MA) -- Faculty of Humanities, Philosophy, 2021
- Full Text:
- Date Issued: 2021-10-29
A critical assessment of the relationship between the AfCFTA and World Trade Organisation dispute settlement mechanisms, in light of the Conflict of Jurisdiction
- Nhemachena, Tichakunda Charles
- Authors: Nhemachena, Tichakunda Charles
- Date: 2021-10-29
- Subjects: African Continental Free Trade Area , World Trade Organization , Dispute resolution (Law) , Conflict of judicial decisions , Good faith (Law)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192052 , vital:45191
- Description: This thesis interrogates the relationship between the Dispute Settlement Understanding and Africa Continental Free Trade Area dispute settlement mechanism, in light of the conflict of jurisdiction. The conflict of jurisdiction is an adverse effect of the fragmentation of international law. The uncoordinated proliferation of international treaties has increased occurrences of overlapping memberships and overlapping subject matter regulation amongst treaties. Whenever the overlaps mentioned above exist, and a dispute arises concerning matters of overlap, that dispute can be heard in more than one tribunal, giving rise to a conflict of jurisdiction. Jurisdictional conflicts are a problem because they breed uncertainty in the adjudication of disputes; they increase the risk of forum shopping, conflict of rulings, protracted litigation, and waste resources. There is a significant risk for jurisdictional conflicts between the World Trade Organisation and Africa Continental Free Trade Area agreements, because of membership and subject matter overlaps. To mitigate the problems caused by jurisdictional conflicts, the Africa Continental Free Trade Area agreement has incorporated a fork-in-the-road clause. Fork-in-the-road provisions allow parties to choose their preferred forum, and once the forum is chosen, the parties are prohibited from bringing the same dispute to another tribunal. Unfortunately, fork-in-the-road clauses are insufficient in resolving jurisdictional conflicts because they do not bind the Dispute Settlement Understanding. It is only bound to enforce World Trade Organisation obligations and not non-World Trade Organisation obligations. The extent to which non-World Trade Organisation norms apply in the Dispute Settlement Understanding is unsettled, making it difficult to conclude whether a fork-in-the-road provision will be effective an effective solution to potential jurisdictional conflicts. In this thesis, the researcher investigates the prospects of the World Trade Organisation applying the AfCFTA fork-in-the-road clause, directly, as a potential solution to the conflict of jurisdiction. In addition, the researcher will also investigate an alternative means of applying the AfCFTA fork-in-the-road provision, indirectly, using the World Trade Organisation procedural good faith provisions. In conclusion, the researcher provides recommendations on how the World Trade Organisation and the AfCFTA agreement can facilitate the application of fork-in-the-road clauses in the Dispute Settlement Understanding to resolve the conflict of jurisdiction. , Thesis (LLM) -- Faculty of Law, Law, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Nhemachena, Tichakunda Charles
- Date: 2021-10-29
- Subjects: African Continental Free Trade Area , World Trade Organization , Dispute resolution (Law) , Conflict of judicial decisions , Good faith (Law)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192052 , vital:45191
- Description: This thesis interrogates the relationship between the Dispute Settlement Understanding and Africa Continental Free Trade Area dispute settlement mechanism, in light of the conflict of jurisdiction. The conflict of jurisdiction is an adverse effect of the fragmentation of international law. The uncoordinated proliferation of international treaties has increased occurrences of overlapping memberships and overlapping subject matter regulation amongst treaties. Whenever the overlaps mentioned above exist, and a dispute arises concerning matters of overlap, that dispute can be heard in more than one tribunal, giving rise to a conflict of jurisdiction. Jurisdictional conflicts are a problem because they breed uncertainty in the adjudication of disputes; they increase the risk of forum shopping, conflict of rulings, protracted litigation, and waste resources. There is a significant risk for jurisdictional conflicts between the World Trade Organisation and Africa Continental Free Trade Area agreements, because of membership and subject matter overlaps. To mitigate the problems caused by jurisdictional conflicts, the Africa Continental Free Trade Area agreement has incorporated a fork-in-the-road clause. Fork-in-the-road provisions allow parties to choose their preferred forum, and once the forum is chosen, the parties are prohibited from bringing the same dispute to another tribunal. Unfortunately, fork-in-the-road clauses are insufficient in resolving jurisdictional conflicts because they do not bind the Dispute Settlement Understanding. It is only bound to enforce World Trade Organisation obligations and not non-World Trade Organisation obligations. The extent to which non-World Trade Organisation norms apply in the Dispute Settlement Understanding is unsettled, making it difficult to conclude whether a fork-in-the-road provision will be effective an effective solution to potential jurisdictional conflicts. In this thesis, the researcher investigates the prospects of the World Trade Organisation applying the AfCFTA fork-in-the-road clause, directly, as a potential solution to the conflict of jurisdiction. In addition, the researcher will also investigate an alternative means of applying the AfCFTA fork-in-the-road provision, indirectly, using the World Trade Organisation procedural good faith provisions. In conclusion, the researcher provides recommendations on how the World Trade Organisation and the AfCFTA agreement can facilitate the application of fork-in-the-road clauses in the Dispute Settlement Understanding to resolve the conflict of jurisdiction. , Thesis (LLM) -- Faculty of Law, Law, 2021
- Full Text:
- Date Issued: 2021-10-29
Application of computer-aided drug design for identification of P. falciparum inhibitors
- Authors: Diallo, Bakary N’tji
- Date: 2021-10-29
- Subjects: Plasmodium falciparum , Malaria -- Chemotherapy , Molecular dynamics , Antimalarials , Cheminformatics , Drug development , Ligand binding (Biochemistry) , Plasmodium falciparum1-deoxy-D-xylulose-5-phosphate reductoisomerase (PfDXR) , South African Natural Compounds Database
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/192798 , vital:45265 , 10.21504/10962/192798
- Description: Malaria is a millennia-old disease with the first recorded cases dating back to 2700 BC found in Chinese medical records, and later in other civilizations. It has claimed human lives to such an extent that there are a notable associated socio-economic consequences. Currently, according to the World Health Organization (WHO), Africa holds the highest disease burden with 94% of deaths and 82% of cases with P. falciparum having ~100% prevalence. Chemotherapy, such as artemisinin combination therapy, has been and continues to be the work horse in the fight against the disease, together with seasonal malaria chemoprevention and the use of insecticides. Natural products such as quinine and artemisinin are particularly important in terms of their antimalarial activity. The emphasis in current chemotherapy research is the need for time and cost-effective workflows focussed on new mechanisms of action (MoAs) covering the target candidate profiles (TCPs). Despite a decline in cases over the past decades with, countries increasingly becoming certified malaria free, a stalling trend has been observed in the past five years resulting in missing the 2020 Global Technical Strategy (GTS) milestones. With no effective vaccine, a reduction in funding, slower drug approval than resistance emergence from resistant and invasive vectors, and threats in diagnosis with the pfhrp2/3 gene deletion, malaria remains a major health concern. Motivated by these reasons, the primary aim of this work was a contribution to the antimalarial pipeline through in silico approaches focusing on P. falciparum. We first intended an exploration of malarial targets through a proteome scale screening on 36 targets using multiple metrics to account for the multi-objective nature of drug discovery. The continuous growth of structural data offers the ideal scenario for mining new MoAs covering antimalarials TCPs. This was combined with a repurposing strategy using a set of orally available FDA approved drugs. Further, use was made of time- and cost-effective strategies combining QVina-W efficiency metrics that integrate molecular properties, GRIM rescoring for molecular interactions and a hydrogen mass repartitioning (HMR) molecular dynamics (MD) scheme for accelerated development of antimalarials in the context of resistance. This pipeline further integrates a complex ranking for better drug-target selectivity, and normalization strategies to overcome docking scoring function bias. The different metrics, ranking, normalization strategies and their combinations were first assessed using their mean ranking error (MRE). A version combining all metrics was used to select 36 unique protein-ligand complexes, assessed in MD, with the final retention of 25. From the 16 in vitro tested hits of the 25, fingolimod, abiraterone, prazosin, and terazosin showed antiplasmodial activity with IC50 2.21, 3.37, 16.67 and 34.72 μM respectively and of these, only fingolimod was found to be not safe with respect to human cell viability. These compounds were predicted active on different molecular targets, abiraterone was predicted to interact with a putative liver-stage essential target, hence promising as a transmission-blocking agent. The pipeline had a promising 25% hit rate considering the proteome-scale and use of cost-effective approaches. Secondly, we focused on Plasmodium falciparum 1-deoxy-D-xylulose-5-phosphate reductoisomerase (PfDXR) using a more extensive screening pipeline to overcome some of the current in silico screening limitations. Starting from the ZINC lead-like library of ~3M, hierarchical ligand-based virtual screening (LBVS) and structure-based virtual screening (SBVS) approaches with molecular docking and re-scoring using eleven scoring functions (SFs) were used. Later ranking with an exponential consensus strategy was included. Selected hits were further assessed through Molecular Mechanics Poisson-Boltzmann Surface Area (MM-PBSA), advanced MD sampling in a ligand pulling simulations and (Weighted Histogram Analysis Method) WHAM analysis for umbrella sampling (US) to derive binding free energies. Four leads had better predicted affinities in US than LC5, a 280 nM potent PfDXR inhibitor with ZINC000050633276 showing a promising binding of -20.43 kcal/mol. As shown with fosmidomycin, DXR inhibition offers fast acting compounds fulfilling antimalarials TCP1. Yet, fosmidomycin has a high polarity causing its short half-life and hampering its clinical use. These leads scaffolds are different from fosmidomycin and hence may offer better pharmacokinetic and pharmacodynamic properties and may also be promising for lead optimization. A combined analysis of residues’ contributions to the free energy of binding in MM-PBSA and to steered molecular dynamics (SMD) Fmax indicated GLU233, CYS268, SER270, TRP296, and HIS341 as exploitable for compound optimization. Finally, we updated the SANCDB library with new NPs and their commercially available analogs as a solution to NP availability. The library is extended to 1005 compounds from its initial 600 compounds and the database is integrated to Mcule and Molport APIs for analogs automatic update. The new set may contribute to virtual screening and to antimalarials as the most effective ones have NP origin. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Diallo, Bakary N’tji
- Date: 2021-10-29
- Subjects: Plasmodium falciparum , Malaria -- Chemotherapy , Molecular dynamics , Antimalarials , Cheminformatics , Drug development , Ligand binding (Biochemistry) , Plasmodium falciparum1-deoxy-D-xylulose-5-phosphate reductoisomerase (PfDXR) , South African Natural Compounds Database
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/192798 , vital:45265 , 10.21504/10962/192798
- Description: Malaria is a millennia-old disease with the first recorded cases dating back to 2700 BC found in Chinese medical records, and later in other civilizations. It has claimed human lives to such an extent that there are a notable associated socio-economic consequences. Currently, according to the World Health Organization (WHO), Africa holds the highest disease burden with 94% of deaths and 82% of cases with P. falciparum having ~100% prevalence. Chemotherapy, such as artemisinin combination therapy, has been and continues to be the work horse in the fight against the disease, together with seasonal malaria chemoprevention and the use of insecticides. Natural products such as quinine and artemisinin are particularly important in terms of their antimalarial activity. The emphasis in current chemotherapy research is the need for time and cost-effective workflows focussed on new mechanisms of action (MoAs) covering the target candidate profiles (TCPs). Despite a decline in cases over the past decades with, countries increasingly becoming certified malaria free, a stalling trend has been observed in the past five years resulting in missing the 2020 Global Technical Strategy (GTS) milestones. With no effective vaccine, a reduction in funding, slower drug approval than resistance emergence from resistant and invasive vectors, and threats in diagnosis with the pfhrp2/3 gene deletion, malaria remains a major health concern. Motivated by these reasons, the primary aim of this work was a contribution to the antimalarial pipeline through in silico approaches focusing on P. falciparum. We first intended an exploration of malarial targets through a proteome scale screening on 36 targets using multiple metrics to account for the multi-objective nature of drug discovery. The continuous growth of structural data offers the ideal scenario for mining new MoAs covering antimalarials TCPs. This was combined with a repurposing strategy using a set of orally available FDA approved drugs. Further, use was made of time- and cost-effective strategies combining QVina-W efficiency metrics that integrate molecular properties, GRIM rescoring for molecular interactions and a hydrogen mass repartitioning (HMR) molecular dynamics (MD) scheme for accelerated development of antimalarials in the context of resistance. This pipeline further integrates a complex ranking for better drug-target selectivity, and normalization strategies to overcome docking scoring function bias. The different metrics, ranking, normalization strategies and their combinations were first assessed using their mean ranking error (MRE). A version combining all metrics was used to select 36 unique protein-ligand complexes, assessed in MD, with the final retention of 25. From the 16 in vitro tested hits of the 25, fingolimod, abiraterone, prazosin, and terazosin showed antiplasmodial activity with IC50 2.21, 3.37, 16.67 and 34.72 μM respectively and of these, only fingolimod was found to be not safe with respect to human cell viability. These compounds were predicted active on different molecular targets, abiraterone was predicted to interact with a putative liver-stage essential target, hence promising as a transmission-blocking agent. The pipeline had a promising 25% hit rate considering the proteome-scale and use of cost-effective approaches. Secondly, we focused on Plasmodium falciparum 1-deoxy-D-xylulose-5-phosphate reductoisomerase (PfDXR) using a more extensive screening pipeline to overcome some of the current in silico screening limitations. Starting from the ZINC lead-like library of ~3M, hierarchical ligand-based virtual screening (LBVS) and structure-based virtual screening (SBVS) approaches with molecular docking and re-scoring using eleven scoring functions (SFs) were used. Later ranking with an exponential consensus strategy was included. Selected hits were further assessed through Molecular Mechanics Poisson-Boltzmann Surface Area (MM-PBSA), advanced MD sampling in a ligand pulling simulations and (Weighted Histogram Analysis Method) WHAM analysis for umbrella sampling (US) to derive binding free energies. Four leads had better predicted affinities in US than LC5, a 280 nM potent PfDXR inhibitor with ZINC000050633276 showing a promising binding of -20.43 kcal/mol. As shown with fosmidomycin, DXR inhibition offers fast acting compounds fulfilling antimalarials TCP1. Yet, fosmidomycin has a high polarity causing its short half-life and hampering its clinical use. These leads scaffolds are different from fosmidomycin and hence may offer better pharmacokinetic and pharmacodynamic properties and may also be promising for lead optimization. A combined analysis of residues’ contributions to the free energy of binding in MM-PBSA and to steered molecular dynamics (SMD) Fmax indicated GLU233, CYS268, SER270, TRP296, and HIS341 as exploitable for compound optimization. Finally, we updated the SANCDB library with new NPs and their commercially available analogs as a solution to NP availability. The library is extended to 1005 compounds from its initial 600 compounds and the database is integrated to Mcule and Molport APIs for analogs automatic update. The new set may contribute to virtual screening and to antimalarials as the most effective ones have NP origin. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Date Issued: 2021-10-29
The construction of phthalocyanine- carbon nanoparticle conjugates for applications in photodynamic therapy and non-linear optics
- Matshitse, Refilwe Manyama Stephina
- Authors: Matshitse, Refilwe Manyama Stephina
- Date: 2021-10-29
- Subjects: Phthalocyanines , Nanodiamonds , Photochemotherapy , Nonlinear optics , Quantum dots
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/188397 , vital:44750 , 10.21504/10962/188397
- Description: The syntheses and characterization of symmetric and asymmetric Pcs functionalized at the peripheral position and sometimes positively charged are reported. The Pcs had either H2, zinc or silicon as central metals and have pyridyloxy, benzothiozole phenoxy, and respective cationic analogues as ring substituents. The Pcs were linked to carbon based nanoparticles such as graphene quantum dots, carbon dots, and detonation nanodiamonds (DNDs) via an ester, amide bond and/or π - π stacking. The physicochemical characteristics of the Pcs were assessed when alone and when in a conjugated system. Both symmetrically and asymmetrically substituted benzothiozole Pcs when quaternised displayed higher triplet and singlet oxygen quantum yields than their unquaternised counterparts. Linkage to carbon nanoparticles (especially to detonation nanodiamonds) had an increasing effect on triplet and singlet oxygen quantum yield. However, a general decrease in singlet oxygen quantum yield on linkage to doped detonation nanodiamonds was associated with the screening effect of DNDs. Heteroatom doped DNDs-Pc nanohybrids have less singlet oxygen than Pcs alone due to molecular structural stability associated with strain that is relatively reduced upon linking Pcs. The In vitro dark cytotoxicity and photodynamic therapy of selected Pc complexes and conjugates against MCF-7 cells was tested. All studied Pc complexes and conjugates showed minimum dark toxicity making them applicable for PDT. When Pc complexes are alone, there is less phototoxicity with >22% cell viability at concentrations ≤ 50 μg/mL relative to conjugates with <22% cell viability at concentrations ≤ 50 μg/mL. There was no direct relationship between PDT and singlet oxygen quantum yields. Nonlinear optical characteristics of complexes was improved upon conjugation of DNDs. Absorbance, input energy, percentage loading, central metal, substituent of Pc and nature of interaction (covalent, noncovalent) are amongst some of the factors that influence nonlinear absorption properties of materials used in this study. All materials followed reverse saturable absorption through two photon absorption mechanism at the excitation wavelength of 532 nm. Aggregates reduce excited state lifetime and Beff under high concentrations/absorbance. A direct relationship between absorbance and Beff of DNDs nanoconjugated systems at low concentrations result in increased optical limiting characteristics of materials. The findings from this work show the importance of linking (nonlinear optics and photodynamic therapy) and doping (photodynamic therapy) photosensitisers such as phthalocyanines and sometimes boron dipyrromethenes onto carbon based nanoparticles for the enhanced characteristics in variable applications. , Thesis (PhD) -- Faculty of Science, Chemistry, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Matshitse, Refilwe Manyama Stephina
- Date: 2021-10-29
- Subjects: Phthalocyanines , Nanodiamonds , Photochemotherapy , Nonlinear optics , Quantum dots
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/188397 , vital:44750 , 10.21504/10962/188397
- Description: The syntheses and characterization of symmetric and asymmetric Pcs functionalized at the peripheral position and sometimes positively charged are reported. The Pcs had either H2, zinc or silicon as central metals and have pyridyloxy, benzothiozole phenoxy, and respective cationic analogues as ring substituents. The Pcs were linked to carbon based nanoparticles such as graphene quantum dots, carbon dots, and detonation nanodiamonds (DNDs) via an ester, amide bond and/or π - π stacking. The physicochemical characteristics of the Pcs were assessed when alone and when in a conjugated system. Both symmetrically and asymmetrically substituted benzothiozole Pcs when quaternised displayed higher triplet and singlet oxygen quantum yields than their unquaternised counterparts. Linkage to carbon nanoparticles (especially to detonation nanodiamonds) had an increasing effect on triplet and singlet oxygen quantum yield. However, a general decrease in singlet oxygen quantum yield on linkage to doped detonation nanodiamonds was associated with the screening effect of DNDs. Heteroatom doped DNDs-Pc nanohybrids have less singlet oxygen than Pcs alone due to molecular structural stability associated with strain that is relatively reduced upon linking Pcs. The In vitro dark cytotoxicity and photodynamic therapy of selected Pc complexes and conjugates against MCF-7 cells was tested. All studied Pc complexes and conjugates showed minimum dark toxicity making them applicable for PDT. When Pc complexes are alone, there is less phototoxicity with >22% cell viability at concentrations ≤ 50 μg/mL relative to conjugates with <22% cell viability at concentrations ≤ 50 μg/mL. There was no direct relationship between PDT and singlet oxygen quantum yields. Nonlinear optical characteristics of complexes was improved upon conjugation of DNDs. Absorbance, input energy, percentage loading, central metal, substituent of Pc and nature of interaction (covalent, noncovalent) are amongst some of the factors that influence nonlinear absorption properties of materials used in this study. All materials followed reverse saturable absorption through two photon absorption mechanism at the excitation wavelength of 532 nm. Aggregates reduce excited state lifetime and Beff under high concentrations/absorbance. A direct relationship between absorbance and Beff of DNDs nanoconjugated systems at low concentrations result in increased optical limiting characteristics of materials. The findings from this work show the importance of linking (nonlinear optics and photodynamic therapy) and doping (photodynamic therapy) photosensitisers such as phthalocyanines and sometimes boron dipyrromethenes onto carbon based nanoparticles for the enhanced characteristics in variable applications. , Thesis (PhD) -- Faculty of Science, Chemistry, 2021
- Full Text:
- Date Issued: 2021-10-29
An interpretative phenomenological analysis of mothers’ experiences raising an autistic child
- Authors: Hewson, Aimee Jessica
- Date: 2021-10-29
- Subjects: Autism spectrum disorders South Africa , Autism spectrum disorders in children South Africa , Mothers of children with disabilities South Africa , Phenomenological psychology , Qualitative research , Interpretative phenomenological analysis
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190725 , vital:45022
- Description: There has been an increasing research focus on Autism Spectrum Disorder (ASD) and the experiences of those affected by autism. Previous research has demonstrated that mothers experience difficulties in all spheres related to their autistic child. Mothers were focused on exclusively in this study as previous research found that it is mostly mothers who take on the primary caring role for their children. As a result, this research aimed to explore the perceptions and experience of six South African mothers raising their ASD child. This research used a qualitative research approach and an interpretative phenomenological analysis methodology. Two semi-structured interviews were held with each participant, with interviews being audio recorded. After analysis, five themes were found, namely: (1) learning their child has a condition or diagnosis, (2) learning and sharing about ASD, (3) the mothering experience, (4) interventions and cost and (5) supportive resources. Careful anonymisation choices were made in this research to ensure the anonymity of participants as best as possible, due to the recruitment through a gatekeeper organisation and snowball sampling. The main findings of this study were that mothers experienced difficulties and challenges as they navigated the medical world in search of a diagnosis for their child. Mothers also reported following their intuition when it came to their children’s care, interventions, and schooling. The passage of time was a significant issue to mothers, as they described several delays and waiting periods. Mothers also described their concerns regarding their children’s future and the fact that their child would be dependent on them and would not be able to form autonomous mature relationships with others. They also reported various thoughts on disclosing their child’s diagnosis to their children. This research, therefore, added to and expanded upon South African literature on the experiences of mothers raising their ASD child. Concurring with previous research, this project found that mothers experience many challenges in the raising of their ASD child. , Thesis (MSocSci) -- Faculty of Humanities, Psychology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Hewson, Aimee Jessica
- Date: 2021-10-29
- Subjects: Autism spectrum disorders South Africa , Autism spectrum disorders in children South Africa , Mothers of children with disabilities South Africa , Phenomenological psychology , Qualitative research , Interpretative phenomenological analysis
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190725 , vital:45022
- Description: There has been an increasing research focus on Autism Spectrum Disorder (ASD) and the experiences of those affected by autism. Previous research has demonstrated that mothers experience difficulties in all spheres related to their autistic child. Mothers were focused on exclusively in this study as previous research found that it is mostly mothers who take on the primary caring role for their children. As a result, this research aimed to explore the perceptions and experience of six South African mothers raising their ASD child. This research used a qualitative research approach and an interpretative phenomenological analysis methodology. Two semi-structured interviews were held with each participant, with interviews being audio recorded. After analysis, five themes were found, namely: (1) learning their child has a condition or diagnosis, (2) learning and sharing about ASD, (3) the mothering experience, (4) interventions and cost and (5) supportive resources. Careful anonymisation choices were made in this research to ensure the anonymity of participants as best as possible, due to the recruitment through a gatekeeper organisation and snowball sampling. The main findings of this study were that mothers experienced difficulties and challenges as they navigated the medical world in search of a diagnosis for their child. Mothers also reported following their intuition when it came to their children’s care, interventions, and schooling. The passage of time was a significant issue to mothers, as they described several delays and waiting periods. Mothers also described their concerns regarding their children’s future and the fact that their child would be dependent on them and would not be able to form autonomous mature relationships with others. They also reported various thoughts on disclosing their child’s diagnosis to their children. This research, therefore, added to and expanded upon South African literature on the experiences of mothers raising their ASD child. Concurring with previous research, this project found that mothers experience many challenges in the raising of their ASD child. , Thesis (MSocSci) -- Faculty of Humanities, Psychology, 2021
- Full Text:
- Date Issued: 2021-10-29
The influence of selected classes of surfactants on microbial fuel cell performance: extensive analysis of microbial activity, bioremediation and power generation for wastewater treatment
- Authors: Lipali, Molopo Johannes
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192493 , vital:45231
- Description: Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Lipali, Molopo Johannes
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192493 , vital:45231
- Description: Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
The roots run deep?: Understanding branch politics within the African National Congress
- Authors: Mkhutle, Nhlanhla Handsome
- Date: 2021-10-29
- Subjects: African National Congress , Manipulative behavior Political aspects South Africa , Cartels South Africa , Power (Social sciences) South Africa , Patronage, Political South Africa , Political party organization South Africa , Branch politics
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190261 , vital:44978
- Description: This thesis explores the functioning of branch politics within the African National Congress (ANC) and the influence of branch politics on the organization and its structures. The ANC has many of the characteristics of a mass party. In mass parties, mass participation is supposed to be promoted through the activities of party branches. This thesis documents the weakening of the ANC’s local level structures and their political control over the organization’s decisions. ANC branches are investigated as democratic vanguards of ANC’s mass party persona, in relation to their functioning as the most basic units of the organization. The thesis reflects on branch members’ experiences in different ANC branches to understand the interplay between branch politics, political power, and patronage politics within the ANC. This research project also explores the possibility of manipulation of ANC branches by senior leaders and would-be-leaders of the ANC. The thesis then concludes by summarising the state of branch politics within the ANC and giving recommendations on further study with regards to branch politics of the ANC. , Thesis (MA) -- Faculty of Humanities, Political and International Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Mkhutle, Nhlanhla Handsome
- Date: 2021-10-29
- Subjects: African National Congress , Manipulative behavior Political aspects South Africa , Cartels South Africa , Power (Social sciences) South Africa , Patronage, Political South Africa , Political party organization South Africa , Branch politics
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190261 , vital:44978
- Description: This thesis explores the functioning of branch politics within the African National Congress (ANC) and the influence of branch politics on the organization and its structures. The ANC has many of the characteristics of a mass party. In mass parties, mass participation is supposed to be promoted through the activities of party branches. This thesis documents the weakening of the ANC’s local level structures and their political control over the organization’s decisions. ANC branches are investigated as democratic vanguards of ANC’s mass party persona, in relation to their functioning as the most basic units of the organization. The thesis reflects on branch members’ experiences in different ANC branches to understand the interplay between branch politics, political power, and patronage politics within the ANC. This research project also explores the possibility of manipulation of ANC branches by senior leaders and would-be-leaders of the ANC. The thesis then concludes by summarising the state of branch politics within the ANC and giving recommendations on further study with regards to branch politics of the ANC. , Thesis (MA) -- Faculty of Humanities, Political and International Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
Fabrication of gold-nanoparticle/conductive polymer composite materials: application to aptamer-based impedimetric biosensors for detection of histamine
- Authors: Ojo, Dupe Ruth
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192550 , vital:45236
- Description: Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Ojo, Dupe Ruth
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192550 , vital:45236
- Description: Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
Photosynthetic microbial fuel cells and fabricated photobioreactors applied with halotolerant microorganisms for nutrient and metal remediation under adverse hypersaline
- Authors: Smith, Galad Gilbert
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192561 , vital:45237
- Description: Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Smith, Galad Gilbert
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192561 , vital:45237
- Description: Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
Genetic connectivity of the roundjaw bonefish (Albula glossodonta) in the Southwest Indian Ocean
- Talma, Sheena Claudia Aisa Lydie
- Authors: Talma, Sheena Claudia Aisa Lydie
- Date: 2021-10-29
- Subjects: Bonefish Mauritius , Bonefish Seychelles , Bonefish Genetics , Bonefish Habitat , Bonefish Geographical distribution , Bonefish Larvae Dispersal , Genetic markers , Cytochrome b , Fish populations Mauritius , Fish populations Seychelles , Marine ecotourism , Saltwater fly fishing , Bonefish fisheries Catch effort
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192174 , vital:45202
- Description: The Southwest Indian Ocean (SWIO) islands of Mauritius and Seychelles are both highly dependent on tourism and fisheries for their economies. One of the growing ecotourism sectors is saltwater fly fishing, an industry based on catch-and-release fishing for a host of species, including bonefishes. Bonefishes (Albula spp.) have received significant research attention in the Pacific and Atlantic Oceans, with only sporadic research conducted in the Indian Ocean. My project aimed to investigate the genetic connectivity of the roundjaw bonefish (Albula glossodonta) in two island states (Seychelles and Mauritius) within the SWIO using a mitochondrial genetic marker (cyt-b) and next generation sequencing (ddRADseq). Samples collected were grouped based on their spatial distribution. The Seychelles consisted of four island groups (Inner Island Group, Aldabra Group, Amirantes and Alphonse Group, and Farquhar Group) whereas Mauritius was represented by one island group (Saint Brandon). Genetic analyses were undertaken between and within each of these groups. Mitochondrial cytochrome-b identified two species of bonefish: Albula glossodonta and Albula oligolepis; the latter was only genetically identified from the Inner Island Group. I hypothesise that this is due to habitat partitioning, with A. oligolepis being a deeper dwelling bonefish species compared to A. glossodonta, which occupies shallow water habitats such as sand flats, atoll lagoons and reef flats. Neutral SNP loci revealed a panmictic pattern of distribution for A. glossodonta throughout the Seychelles Island groups but showed a pattern of weak structure between Seychelles and Mauritius. Genetic diversity indices such as allelic richness, showed low diversity across the sampling sites (AR range: 1.761-1.889). Population structure tests such as pairwise FST showed low but significant population structure. The highest FST indices were recorded between the Aldabra and Farquhar Groups, as well as the Aldabra and Saint Brandon Groups (0.044 ± 0.000 and 0.040 ± 0.000, respectively). Descriptive tests such as PCA and DAPC showed similar trends, whereby Saint Brandon clustered separately from the other samples from the Seychelles Island groups. However, these trends were Abstract seen at very low variations (PCA axes 1 and 2 accounted for only 2.0 and 1.9 % of the total variation, respectively). A population assignment test grouped the individuals as one ancestral population. A spatial principal component analysis showed that Saint Brandon was dissimilar to the Seychelles Island groups. Like other Elopomorph species, bonefishes have leptocephalus larvae capable of long-distance dispersal which may explain the well-mixed genetic population observed within the Seychelles islands. Although currents within the Indian Ocean, especially on a mesoscale, are not well understood, the South Equatorial Current likely facilitates connectivity between the Seychelles islands while also limiting gene flow between Seychelles and Mauritius. Understanding population structure is important for informing the appropriate management and conservation strategies, especially in oceanic nations where data informing important industries like tourism and fisheries are often limited. The bonefish fly fishing industry is well-known to be a lucrative sector, generating, for example US$ 1.4 million a year in the Bahamas. This study recognised that there are numerous knowledge gaps relevant to the bonefish industry that need to be addressed, including: 1) understanding the socio-economic importance of fly fishing to island states like Seychelles, 2) estimating the abundance and species distribution of bonefishes within Seychelles, 3) understanding effectiveness of MPAs for recreational fishery species like bonefish and, lastly, 4) generating more fishery-relevant biological information on the heavily targeted fly fishing species within Seychelles. These needs must be met to inform management plans and to better manage the fly fishing ventures that target species like bonefish. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Talma, Sheena Claudia Aisa Lydie
- Date: 2021-10-29
- Subjects: Bonefish Mauritius , Bonefish Seychelles , Bonefish Genetics , Bonefish Habitat , Bonefish Geographical distribution , Bonefish Larvae Dispersal , Genetic markers , Cytochrome b , Fish populations Mauritius , Fish populations Seychelles , Marine ecotourism , Saltwater fly fishing , Bonefish fisheries Catch effort
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192174 , vital:45202
- Description: The Southwest Indian Ocean (SWIO) islands of Mauritius and Seychelles are both highly dependent on tourism and fisheries for their economies. One of the growing ecotourism sectors is saltwater fly fishing, an industry based on catch-and-release fishing for a host of species, including bonefishes. Bonefishes (Albula spp.) have received significant research attention in the Pacific and Atlantic Oceans, with only sporadic research conducted in the Indian Ocean. My project aimed to investigate the genetic connectivity of the roundjaw bonefish (Albula glossodonta) in two island states (Seychelles and Mauritius) within the SWIO using a mitochondrial genetic marker (cyt-b) and next generation sequencing (ddRADseq). Samples collected were grouped based on their spatial distribution. The Seychelles consisted of four island groups (Inner Island Group, Aldabra Group, Amirantes and Alphonse Group, and Farquhar Group) whereas Mauritius was represented by one island group (Saint Brandon). Genetic analyses were undertaken between and within each of these groups. Mitochondrial cytochrome-b identified two species of bonefish: Albula glossodonta and Albula oligolepis; the latter was only genetically identified from the Inner Island Group. I hypothesise that this is due to habitat partitioning, with A. oligolepis being a deeper dwelling bonefish species compared to A. glossodonta, which occupies shallow water habitats such as sand flats, atoll lagoons and reef flats. Neutral SNP loci revealed a panmictic pattern of distribution for A. glossodonta throughout the Seychelles Island groups but showed a pattern of weak structure between Seychelles and Mauritius. Genetic diversity indices such as allelic richness, showed low diversity across the sampling sites (AR range: 1.761-1.889). Population structure tests such as pairwise FST showed low but significant population structure. The highest FST indices were recorded between the Aldabra and Farquhar Groups, as well as the Aldabra and Saint Brandon Groups (0.044 ± 0.000 and 0.040 ± 0.000, respectively). Descriptive tests such as PCA and DAPC showed similar trends, whereby Saint Brandon clustered separately from the other samples from the Seychelles Island groups. However, these trends were Abstract seen at very low variations (PCA axes 1 and 2 accounted for only 2.0 and 1.9 % of the total variation, respectively). A population assignment test grouped the individuals as one ancestral population. A spatial principal component analysis showed that Saint Brandon was dissimilar to the Seychelles Island groups. Like other Elopomorph species, bonefishes have leptocephalus larvae capable of long-distance dispersal which may explain the well-mixed genetic population observed within the Seychelles islands. Although currents within the Indian Ocean, especially on a mesoscale, are not well understood, the South Equatorial Current likely facilitates connectivity between the Seychelles islands while also limiting gene flow between Seychelles and Mauritius. Understanding population structure is important for informing the appropriate management and conservation strategies, especially in oceanic nations where data informing important industries like tourism and fisheries are often limited. The bonefish fly fishing industry is well-known to be a lucrative sector, generating, for example US$ 1.4 million a year in the Bahamas. This study recognised that there are numerous knowledge gaps relevant to the bonefish industry that need to be addressed, including: 1) understanding the socio-economic importance of fly fishing to island states like Seychelles, 2) estimating the abundance and species distribution of bonefishes within Seychelles, 3) understanding effectiveness of MPAs for recreational fishery species like bonefish and, lastly, 4) generating more fishery-relevant biological information on the heavily targeted fly fishing species within Seychelles. These needs must be met to inform management plans and to better manage the fly fishing ventures that target species like bonefish. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2021
- Full Text:
- Date Issued: 2021-10-29