- Title
- Forced degradation studies of clobetasol 17‐propionate in methanol, propylene glycol, as bulk drug and cream formulations by RP‐HPLC
- Creator
- Fauzee, Ayesha F, Walker, Roderick B
- Subject
- To be catalogued
- Date
- 2013
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/184005
- Identifier
- vital:44154
- Identifier
- xlink:href="https://doi.org/10.1002/jssc.201200969"
- Description
- A rapid, simple, stability-indicating forced degradation study of clobetasol 17-propionate was conducted using RP-HPLC. The method was used to analyze clobetasol 17-propionate in methanol, propylene glycol, and a cream formulation. Isocratic elution of clobetasol and its degradation products was achieved using a Nova-Pak® 4 μm C18 150 mm × 3.9 mm id cartridge column and a mobile phase of methanol: water (68:32 v/v) at a flow rate of 0.9 mL min−1. Quantitation was achieved with UV detection at 239 nm. Nondegraded clobetasol was eluted at a retention time of 6.0 min. Clobetasol 17-propionate was subjected to different stress conditions viz., acidic, basic, heat, oxidation, light, and neutral hydrolysis. The greatest degradation occurred under strong base and oxidative conditions. Strong base-degraded clobetasol produced additional peaks at retention times of 1.8, 4.0, 5.0, and 8.0 min and clobetasol oxidation degradation peaks eluted at 2.2 and 24 min. Complete validation was performed for linearity, accuracy, and precision over the concentration range 0.15–15 μg mL−1. All data were analyzed statistically and this RP-HPLC method proved to be accurate, precise, linear, and stability indicating for the quantitation of clobetasol 17-propionate in methanol, propylene glycol, and cream formulations.
- Format
- computer, online resource, application/pdf, 1 online resource (8 pages), pdf
- Publisher
- Wiley Library Online
- Language
- English
- Relation
- Journal of Separation Science, Fauzee, A.F.B. and Walker, R.B., 2013. Forced degradation studies of clobetasol 17‐propionate in methanol, propylene glycol, as bulk drug and cream formulations by RP‐HPLC. Journal of separation science, 36(5), pp.849-856, Journal of Separation Science volume 36 number 5 p.849 2013 1615-9314
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Wiley Library Online Terms of Use Statement (https://onlinelibrary.wiley.com/terms-and-conditions)
- Rights
- Closed Access
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Collections
Walker, Roderick Bryan (Prof)
RU Department of Pharmacy
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| View Details Download | SOURCE1 | Forced degradation studies of clobetasol 17‐propionate in methanol, propylene glycol, as bulk drug and cream formulations by RP‐HPLC.pdf | 688 KB | Adobe Acrobat PDF | View Details Download |