A study of four-dimensional oscillator groups and the associated left-invariant control affine systems
- Authors: Biggs, Rory
- Date: 2012
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/164941 , vital:41186
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Department of Mathematics, 2012
- Full Text:
- Date Issued: 2012
- Authors: Biggs, Rory
- Date: 2012
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/164941 , vital:41186
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Department of Mathematics, 2012
- Full Text:
- Date Issued: 2012
Ritualised discourse practices of feedback in a university foundation programme: a critical investigation
- Authors: Richardson, Amy
- Date: 2012
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MA
- Identifier: http://hdl.handle.net/10962/164955 , vital:41187
- Description: In order for students to become true members of academic communities of practice they need epistemological access, including guidance on central institutional knowledge-producing processes and mastery of key academic literacy practices. A powerful source of guidance is marker feedback. Drawing on key insights from the New Literacy Studies and taking up the mandate of Critical Ethnography to improve the status quo, this thesis reports on the feedback practices in a university foundation programme. The findings are based on three micro-case studies compiled and analysed by means of methods drawn from Ethnography, Sociolinguistics and Critical Analysis in conjunction with an expanded, multimodal, APPRAISAL analysis, including adjusted categories and the author’s own feedback typologies. Two major arguments emerge: the feedback provided amounts to a set of ritualised discourse practices and its effects can be likened to the product of the children’s game of Head-body-tail. Consequently, feedback conventions are opaque and, potentially, impede epistemological access. They further entrench five sets of ideologies: (1) Students must master basic English literacy before they are coached in more complex issues such as argumentation; an assumption which leads to differential socialisation. (2) There is a single set of literacy practices that is rewarded. (3) Students have different levels of authorial authority depending on their language abilities. (4) ‘Middle students’ may benefit the most from feedback. (5) Specific comments are preferred over general ones. Analysis of feedback, furthermore, shows that markers’ frames of reference shape their pedagogy and that draft and final versions of work may be framed differently by markers. These findings require a response and, in order to facilitate epistemological access, suggestions are made for improved marker training based on the problematisation of the ritual involved in marking students’ work in this university foundation programme. , Thesis (MA)--Rhodes University, Faculty of Humanities, Linguistics, 2012
- Full Text:
- Date Issued: 2012
- Authors: Richardson, Amy
- Date: 2012
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MA
- Identifier: http://hdl.handle.net/10962/164955 , vital:41187
- Description: In order for students to become true members of academic communities of practice they need epistemological access, including guidance on central institutional knowledge-producing processes and mastery of key academic literacy practices. A powerful source of guidance is marker feedback. Drawing on key insights from the New Literacy Studies and taking up the mandate of Critical Ethnography to improve the status quo, this thesis reports on the feedback practices in a university foundation programme. The findings are based on three micro-case studies compiled and analysed by means of methods drawn from Ethnography, Sociolinguistics and Critical Analysis in conjunction with an expanded, multimodal, APPRAISAL analysis, including adjusted categories and the author’s own feedback typologies. Two major arguments emerge: the feedback provided amounts to a set of ritualised discourse practices and its effects can be likened to the product of the children’s game of Head-body-tail. Consequently, feedback conventions are opaque and, potentially, impede epistemological access. They further entrench five sets of ideologies: (1) Students must master basic English literacy before they are coached in more complex issues such as argumentation; an assumption which leads to differential socialisation. (2) There is a single set of literacy practices that is rewarded. (3) Students have different levels of authorial authority depending on their language abilities. (4) ‘Middle students’ may benefit the most from feedback. (5) Specific comments are preferred over general ones. Analysis of feedback, furthermore, shows that markers’ frames of reference shape their pedagogy and that draft and final versions of work may be framed differently by markers. These findings require a response and, in order to facilitate epistemological access, suggestions are made for improved marker training based on the problematisation of the ritual involved in marking students’ work in this university foundation programme. , Thesis (MA)--Rhodes University, Faculty of Humanities, Linguistics, 2012
- Full Text:
- Date Issued: 2012
Homecoming
- Authors: Mazwai, Nontsikelelo
- Date: 2017
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MA
- Identifier: http://hdl.handle.net/10962/164930 , vital:41185
- Description: Thesis (MA)--Rhodes University, Faculty of Humanities, School of Languages, 2017
- Full Text:
- Date Issued: 2017
- Authors: Mazwai, Nontsikelelo
- Date: 2017
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MA
- Identifier: http://hdl.handle.net/10962/164930 , vital:41185
- Description: Thesis (MA)--Rhodes University, Faculty of Humanities, School of Languages, 2017
- Full Text:
- Date Issued: 2017
A social realist study of employability development in engineering education
- Authors: Nudelman, Gabrielle
- Date: 2018
- Subjects: Critical realism , Electrical engineering -- Study and teaching (Higher) -- South Africa -- Cape Town , Employability , Career education -- South Africa -- Cape Town , School-to-work transition -- South Africa -- Cape Town
- Language: English
- Type: thesis , text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62884 , vital:28307
- Description: This qualitative case study of a course pairing offered to final-year electrical engineering students at the University of Cape Town in 2015 was undertaken in order to better understand the ways in which participation in undergraduate courses can prepare engineering students for the workplace. The course pairing consisted of New Venture Planning and Professional Communication Studies. While the former aimed to expose students to the knowledge relating to starting a new business, the latter focused on teaching students how to create written and oral texts to support such an endeavour. Using Roy Bhaskar’s critical realism as a theoretical underlabourer, the study develops understandings regarding the generative mechanisms at work during the two courses. In support of this, the study posits an understanding of employability that moves beyond the acquisition of discrete workplace skills. Rather, employability is conceptualised as discursive transformation, with students being deemed “work-ready” when they develop discursive identities as engineers. Data generation took place by means of focus group and individual interviews, ethnographic observation and documentary research. Margaret Archer’s social realist tools – in particular, analytical dualism and the morphogenetic framework were used to trace the students’ transformations over the course pairing. It was argued that those students who developed discursive identities of engineers were those who, in Archer’s terms, emerged as social actors at the end of the course pairing. Two characteristics of the courses were found to enable this transformation: those parts that promoted deepened understanding of what the role of “engineer” entailed and the parts that provided spaces for students to develop their own personal identities. The findings of the study indicated that discursive identities as engineers were more likely to be developed through the group work and spaces for reflection engendered by the courses than as a result of the formal curriculum. The implications of the research are that, while a focus on employability in engineering education is valid and productive, this needs to be supported by opportunities for authentic learning experiences which afford students the opportunity to engage in learning that promotes real-life application of knowledge. , Thesis (PhD)--Rhodes University, Faculty of Education, Education, 2018
- Full Text:
- Date Issued: 2018
- Authors: Nudelman, Gabrielle
- Date: 2018
- Subjects: Critical realism , Electrical engineering -- Study and teaching (Higher) -- South Africa -- Cape Town , Employability , Career education -- South Africa -- Cape Town , School-to-work transition -- South Africa -- Cape Town
- Language: English
- Type: thesis , text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62884 , vital:28307
- Description: This qualitative case study of a course pairing offered to final-year electrical engineering students at the University of Cape Town in 2015 was undertaken in order to better understand the ways in which participation in undergraduate courses can prepare engineering students for the workplace. The course pairing consisted of New Venture Planning and Professional Communication Studies. While the former aimed to expose students to the knowledge relating to starting a new business, the latter focused on teaching students how to create written and oral texts to support such an endeavour. Using Roy Bhaskar’s critical realism as a theoretical underlabourer, the study develops understandings regarding the generative mechanisms at work during the two courses. In support of this, the study posits an understanding of employability that moves beyond the acquisition of discrete workplace skills. Rather, employability is conceptualised as discursive transformation, with students being deemed “work-ready” when they develop discursive identities as engineers. Data generation took place by means of focus group and individual interviews, ethnographic observation and documentary research. Margaret Archer’s social realist tools – in particular, analytical dualism and the morphogenetic framework were used to trace the students’ transformations over the course pairing. It was argued that those students who developed discursive identities of engineers were those who, in Archer’s terms, emerged as social actors at the end of the course pairing. Two characteristics of the courses were found to enable this transformation: those parts that promoted deepened understanding of what the role of “engineer” entailed and the parts that provided spaces for students to develop their own personal identities. The findings of the study indicated that discursive identities as engineers were more likely to be developed through the group work and spaces for reflection engendered by the courses than as a result of the formal curriculum. The implications of the research are that, while a focus on employability in engineering education is valid and productive, this needs to be supported by opportunities for authentic learning experiences which afford students the opportunity to engage in learning that promotes real-life application of knowledge. , Thesis (PhD)--Rhodes University, Faculty of Education, Education, 2018
- Full Text:
- Date Issued: 2018
Exploration of challenges in bringing traditional medicine into SA’s healthcare system, using medicinal plants for treatment of waterborne diarrhoeal diseases as a case study
- Authors: Keche, Priscilla
- Date: 2019
- Subjects: Traditional medicine -- South Africa , Waterborne infection -- South Africa , Diarrhea -- South Africa , Healers -- South Africa , Medical care -- Quality control , Medicinal plants -- South Africa , Diarrhea in children -- South Africa , World Health Organization
- Language: English
- Type: thesis , text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/118035 , vital:34588
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Biotechnology Innovation Centre (RUBIC), 2019.
- Full Text:
- Date Issued: 2019
- Authors: Keche, Priscilla
- Date: 2019
- Subjects: Traditional medicine -- South Africa , Waterborne infection -- South Africa , Diarrhea -- South Africa , Healers -- South Africa , Medical care -- Quality control , Medicinal plants -- South Africa , Diarrhea in children -- South Africa , World Health Organization
- Language: English
- Type: thesis , text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/118035 , vital:34588
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Biotechnology Innovation Centre (RUBIC), 2019.
- Full Text:
- Date Issued: 2019
The Trope of the Child: Rereading Trauma, Subjectivity and Embodiment in Contemporary Child-Centred African Narratives by Ahmadou Kourouma, Chris Abani, K. Sello Duiker and Yvonne Vera
- Authors: Njovane, Thandokazi
- Date: 2019
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164557 , vital:41134 , doi:10.21504/10962/164557
- Description: Thesis (PhD)--Rhodes University, Humanities, Literary Studies in English, 2019
- Full Text:
- Date Issued: 2019
- Authors: Njovane, Thandokazi
- Date: 2019
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164557 , vital:41134 , doi:10.21504/10962/164557
- Description: Thesis (PhD)--Rhodes University, Humanities, Literary Studies in English, 2019
- Full Text:
- Date Issued: 2019
An evaluation of the cytotoxic activities of novel artemisinin derivatives: towards targeted therapies for triple-negative breast cancers (TNBC)
- Authors: Kajewole, Deborah Ifeoluwa
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/163329 , vital:41029 , doi:10.21504/10962/163329
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020.
- Full Text:
- Date Issued: 2020
- Authors: Kajewole, Deborah Ifeoluwa
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/163329 , vital:41029 , doi:10.21504/10962/163329
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020.
- Full Text:
- Date Issued: 2020
An investigation into the application of Distributed Endpoint Processing to 3D Immersive Audio Rendering
- Authors: Devonport, Robin Sean
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/163258 , vital:41022
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Computer Science, 2020.
- Full Text:
- Date Issued: 2020
- Authors: Devonport, Robin Sean
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/163258 , vital:41022
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Computer Science, 2020.
- Full Text:
- Date Issued: 2020
Analysis of the regulation of HSP90α expression upon differentiation of C2C12 cells
- Authors: Holm, Nathan Christopher
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/163318 , vital:41028
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020.
- Full Text:
- Date Issued: 2020
- Authors: Holm, Nathan Christopher
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/163318 , vital:41028
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020.
- Full Text:
- Date Issued: 2020
Application of In Vitro Release Testing (IVRT) and a dermatopharmacokinetic approach (tape stripping) for the assessment of Metronidazole topical formulations
- Rath, Seeprarani Prabirkumar
- Authors: Rath, Seeprarani Prabirkumar
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164617 , vital:41148 , doi:10.21504/10962/164617
- Description: Thesis (PhD)--Rhodes University, Faculty of Pharmacy, Pharmaceutics, 2020
- Full Text:
- Date Issued: 2020
- Authors: Rath, Seeprarani Prabirkumar
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164617 , vital:41148 , doi:10.21504/10962/164617
- Description: Thesis (PhD)--Rhodes University, Faculty of Pharmacy, Pharmaceutics, 2020
- Full Text:
- Date Issued: 2020
Assessment of the microbial quality of various domestic rainwater harvesting systems and the suitability of a nano based treatment method
- Authors: Malema, Mokaba Shirley
- Date: 2020
- Language: English
- Type: thesis , text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/110218 , vital:33249 , https://dx.doi.org/10.21504/10962/110218
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Chemistry, 2020.
- Full Text: false
- Date Issued: 2020
- Authors: Malema, Mokaba Shirley
- Date: 2020
- Language: English
- Type: thesis , text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/110218 , vital:33249 , https://dx.doi.org/10.21504/10962/110218
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Chemistry, 2020.
- Full Text: false
- Date Issued: 2020
Bioinformatic analysis of Aminoacyl tRNA Synthetases as potential antimalarial drug targets
- Authors: Nyamai, Dorothy Wavinya
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164579 , vital:41142 , doi:10.21504/10962/164579
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020
- Full Text:
- Date Issued: 2020
- Authors: Nyamai, Dorothy Wavinya
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164579 , vital:41142 , doi:10.21504/10962/164579
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020
- Full Text:
- Date Issued: 2020
BODIPY dyes for use in nonlinear optics and optical sensing
- Authors: Kubheka, Gugu Patience
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/163352 , vital:41032 , doi:10.21504/10962/163352
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Department of Chemistry, 2020.
- Full Text:
- Date Issued: 2020
- Authors: Kubheka, Gugu Patience
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/163352 , vital:41032 , doi:10.21504/10962/163352
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Department of Chemistry, 2020.
- Full Text:
- Date Issued: 2020
CO2-induced woody thickening depends strongly on interacting abiotic and biotic factors
- Authors: Raubenheimer, Sarah Lynn
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164646 , vital:41151 , doi:10.21504/10962/164646
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Botany, 2020
- Full Text:
- Date Issued: 2020
- Authors: Raubenheimer, Sarah Lynn
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164646 , vital:41151 , doi:10.21504/10962/164646
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Botany, 2020
- Full Text:
- Date Issued: 2020
Comparative analysis of the known Hop1b and the novel Hop1a isoforms of the Hop gene
- Authors: Makhubu, Portia
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164311 , vital:41108 , doi:10.21504/10962/164311
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020
- Full Text:
- Date Issued: 2020
- Authors: Makhubu, Portia
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164311 , vital:41108 , doi:10.21504/10962/164311
- Description: Thesis (PhD)--Rhodes University, Faculty of Science, Biochemistry and Microbiology, 2020
- Full Text:
- Date Issued: 2020
Design of Immunobiosensors for Detection of Tumor-Associated Anti-P53 Autoantibodies: Method Development
- Authors: Adeniyi, Omotayo Kayode
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/162988 , vital:41002 , 10.21504/10962/162988
- Description: Introduction -- Experimental -- Label-Free Impedimetric Sensing of Anti-P53ab... -- Fluorescent detection of Anti-P53ab -- Peroxidase-like activity of Fe3O4@SiNP-APTES-Au@Pd... -- Colorimetric detection of Anti-P53ab , Thesis (PhD)--Rhodes University, Science Faculty, Department of Chemistry, 2020. , Detection and profiling of circulating tumor-associated autoantibodies (TAAbs) are useful for screening and early-stage diagnosis of asymptomatic lung cancer. Immunobiosensor technologies aimed to accomplish the highly sensitive, rapid and low-cost detection of TAAbs can improve the early-stage detection of lung cancer. Immunobiosensors for the detection of anti-P53-tumour associated autoantibodies have been developed in this work. The design of sensing interfaces with immobilized P53 protein (P53ag) as a sensing element layer on a solid interface was investigated. Several methods of detecting anti-P53-antibodies (anti-P53ab) were investigated. These methods are label-free detection using electrochemical impedance spectroscopy (EIS) and two label techniques. The label-free electrochemical techniques utilize gold electrode pre-modified with a conducting layer of electrochemically grafted phenylethylamine for covalent immobilization of P53ag. The limit of anti-P53ab detection with the label-free EIS was 103.0 pg.ml-1. The labeled technique developed utilizes fluorescent, and peroxidase-like nanomaterial labeled antibody as a detection probe. For the fluorescence detection, fluorescent silica nanoparticles were synthesized by overloading FITC into the silica matrix and conjugated to detection antibody (anti-IgG). The detection of the anti-P53ab was based on the dissolution of the silica nanoparticles to release the loaded dye as a signal amplification strategy. The fluorescence detection was carried out on a microplate, and magnetic bead modified P53-antigen platforms and limit of detection (LoD) were 42.0 fg.ml-1 and 3.3 fg.ml-1 for anti-P53ab; respectively. Fe3O4@SiNP-APTES-Au@Pd hybrid nanoparticles were synthesized, and their peroxidase-like activity and colorimetric detection were evaluated. The Fe3O4@SiNP-APTES-Au@Pd exhibited comparable activity to HRP. The Fe3O4@SiNP-APTES-Au@Pd was conjugated to protein-G-anti-IgG for the detection of anti-P53ab on a microplate and cellulose paper platforms. The LoD was 20.0 fg.ml-1 and 63.0 fg.ml-1 for the microplate and cellulose paper platform; respectively. The potential application of the designed immunobiosensor was evaluated in simulated serum samples. The developed sensors showed higher detection sensitivity, stability and had a lower detection limit for anti-P53ab when compared with the ELISA based detection. The results have provided alternative and effective quantification approaches to ELISA and a promising future for multiplexed detection of tumor-associated autoantibodies. The developed methodologies in this thesis could be applied for the detection of other autoantibodies in other cancer types and auto-immune diseases.
- Full Text:
- Date Issued: 2020
- Authors: Adeniyi, Omotayo Kayode
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/162988 , vital:41002 , 10.21504/10962/162988
- Description: Introduction -- Experimental -- Label-Free Impedimetric Sensing of Anti-P53ab... -- Fluorescent detection of Anti-P53ab -- Peroxidase-like activity of Fe3O4@SiNP-APTES-Au@Pd... -- Colorimetric detection of Anti-P53ab , Thesis (PhD)--Rhodes University, Science Faculty, Department of Chemistry, 2020. , Detection and profiling of circulating tumor-associated autoantibodies (TAAbs) are useful for screening and early-stage diagnosis of asymptomatic lung cancer. Immunobiosensor technologies aimed to accomplish the highly sensitive, rapid and low-cost detection of TAAbs can improve the early-stage detection of lung cancer. Immunobiosensors for the detection of anti-P53-tumour associated autoantibodies have been developed in this work. The design of sensing interfaces with immobilized P53 protein (P53ag) as a sensing element layer on a solid interface was investigated. Several methods of detecting anti-P53-antibodies (anti-P53ab) were investigated. These methods are label-free detection using electrochemical impedance spectroscopy (EIS) and two label techniques. The label-free electrochemical techniques utilize gold electrode pre-modified with a conducting layer of electrochemically grafted phenylethylamine for covalent immobilization of P53ag. The limit of anti-P53ab detection with the label-free EIS was 103.0 pg.ml-1. The labeled technique developed utilizes fluorescent, and peroxidase-like nanomaterial labeled antibody as a detection probe. For the fluorescence detection, fluorescent silica nanoparticles were synthesized by overloading FITC into the silica matrix and conjugated to detection antibody (anti-IgG). The detection of the anti-P53ab was based on the dissolution of the silica nanoparticles to release the loaded dye as a signal amplification strategy. The fluorescence detection was carried out on a microplate, and magnetic bead modified P53-antigen platforms and limit of detection (LoD) were 42.0 fg.ml-1 and 3.3 fg.ml-1 for anti-P53ab; respectively. Fe3O4@SiNP-APTES-Au@Pd hybrid nanoparticles were synthesized, and their peroxidase-like activity and colorimetric detection were evaluated. The Fe3O4@SiNP-APTES-Au@Pd exhibited comparable activity to HRP. The Fe3O4@SiNP-APTES-Au@Pd was conjugated to protein-G-anti-IgG for the detection of anti-P53ab on a microplate and cellulose paper platforms. The LoD was 20.0 fg.ml-1 and 63.0 fg.ml-1 for the microplate and cellulose paper platform; respectively. The potential application of the designed immunobiosensor was evaluated in simulated serum samples. The developed sensors showed higher detection sensitivity, stability and had a lower detection limit for anti-P53ab when compared with the ELISA based detection. The results have provided alternative and effective quantification approaches to ELISA and a promising future for multiplexed detection of tumor-associated autoantibodies. The developed methodologies in this thesis could be applied for the detection of other autoantibodies in other cancer types and auto-immune diseases.
- Full Text:
- Date Issued: 2020
Design of immunosensor for the detection of C-reactive protein using oriented antibody immobilization
- Authors: Adesina, Abiola Olanike
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/163080 , vital:41010 , https://dx.doi.org/10.21504/10962/163080
- Description: Thesis (PhD)--Rhodes University, Science Faculty, Department of Chemistry, 2020.
- Full Text:
- Date Issued: 2020
- Authors: Adesina, Abiola Olanike
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/163080 , vital:41010 , https://dx.doi.org/10.21504/10962/163080
- Description: Thesis (PhD)--Rhodes University, Science Faculty, Department of Chemistry, 2020.
- Full Text:
- Date Issued: 2020
Development and assessment of a fixed dose combination of perindopril arginine and indapamide loaded microparticles
- Authors: Mandava, Tavonga Tyomai
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/164413 , vital:41116
- Description: Thesis (MSc)--Rhodes University, Faculty of Pharmacy, Pharmaceutics, 2020
- Full Text:
- Date Issued: 2020
- Authors: Mandava, Tavonga Tyomai
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/164413 , vital:41116
- Description: Thesis (MSc)--Rhodes University, Faculty of Pharmacy, Pharmaceutics, 2020
- Full Text:
- Date Issued: 2020
Development of a low-cost bioprinting system for engineering of Human Tumour Models
- Authors: Fanucci, Sidne
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/163295 , vital:41026
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Biotechnology Innovation Centre, 2020.
- Full Text:
- Date Issued: 2020
- Authors: Fanucci, Sidne
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/163295 , vital:41026
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Biotechnology Innovation Centre, 2020.
- Full Text:
- Date Issued: 2020
Development of a protocol for extracting and quantifying the concentration of thiafentanil in blesbok (Damaliscus pygargus phillipsi) matrices 72-74 hours post administration
- Authors: Webber, Judith Tracy
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/164738 , vital:41159
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Chemistry, 2020
- Full Text:
- Date Issued: 2020
- Authors: Webber, Judith Tracy
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/164738 , vital:41159
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Chemistry, 2020
- Full Text:
- Date Issued: 2020