Depressive disorders and chronic comorbid disease states: a pharmacoepidemiological evaluation
- Authors: Kritiotis, Lia Costas
- Date: 2007
- Subjects: Depression, Mental , Child psychopathology , Adolescent psychopathology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10151 , http://hdl.handle.net/10948/653 , Depression, Mental , Child psychopathology , Adolescent psychopathology
- Description: The treatment of Depressive Disorders in patients with chronic comorbid disease states warrants careful consideration of the risk-benefit ratio pertaining to the pharmacokinetic and pharmacodynamic characteristics of the antidepressant being considered, against the physiological susceptibilities of the patient; potential drug-drug interactions and depressive symptoms. The primary aim of this study was to investigate the relationship between Depressive Disorders and the most frequently diagnosed chronic comorbid disease states in a depressed South African study population; and to comment on the appropriateness of antidepressants prescribed to these patients. This retrospective drug utilisation study consisted of two parts: the first part focused on the prevalence of the most frequently diagnosed chronic comorbidities in a depressed South African population (N = 21 171). The three most prevalent chronic comorbid disease states were Hypertension (52.87 percent), Lipid Disorders (20.40 percent) and Arthritis (16.70 percent). The second part of the study included an assessment of the antidepressants prescribed to depressed patients in 2004 (N = 6 150). Emphasis was placed on the suitability of antidepressants selected for depressed adult patients (18 years of age and older) with comorbid Hypertension, Lipid Disorders or Arthritis. SSRIs were prescribed most frequently to the depressed patients during 2004 (59.67 percent). SSRIs are the suggested first-line treatments for depressed patients with multiple chronic comorbid disease states. However, of the SSRIs, fluoxetine has the least favourable pharmacokinetic profile and was found to be the antidepressant prescribed most often. Amitriptyline, which was the TCA prescribed most frequently, produces the greatest degree of anticholinergic, sedative and hypotensive effects, relative to other agents in the same antidepressant class. Thus, it is not recommended as a first-line antidepressant, especially in depressed patients with comorbidities. This study identifies potential areas of intervention regarding antidepressant prescribing in depressed individuals with chronic comorbid disease states and offers recommendations to promote the selection of optimal, individualised drug treatment strategies for these patients.
- Full Text:
- Date Issued: 2007
- Authors: Kritiotis, Lia Costas
- Date: 2007
- Subjects: Depression, Mental , Child psychopathology , Adolescent psychopathology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10151 , http://hdl.handle.net/10948/653 , Depression, Mental , Child psychopathology , Adolescent psychopathology
- Description: The treatment of Depressive Disorders in patients with chronic comorbid disease states warrants careful consideration of the risk-benefit ratio pertaining to the pharmacokinetic and pharmacodynamic characteristics of the antidepressant being considered, against the physiological susceptibilities of the patient; potential drug-drug interactions and depressive symptoms. The primary aim of this study was to investigate the relationship between Depressive Disorders and the most frequently diagnosed chronic comorbid disease states in a depressed South African study population; and to comment on the appropriateness of antidepressants prescribed to these patients. This retrospective drug utilisation study consisted of two parts: the first part focused on the prevalence of the most frequently diagnosed chronic comorbidities in a depressed South African population (N = 21 171). The three most prevalent chronic comorbid disease states were Hypertension (52.87 percent), Lipid Disorders (20.40 percent) and Arthritis (16.70 percent). The second part of the study included an assessment of the antidepressants prescribed to depressed patients in 2004 (N = 6 150). Emphasis was placed on the suitability of antidepressants selected for depressed adult patients (18 years of age and older) with comorbid Hypertension, Lipid Disorders or Arthritis. SSRIs were prescribed most frequently to the depressed patients during 2004 (59.67 percent). SSRIs are the suggested first-line treatments for depressed patients with multiple chronic comorbid disease states. However, of the SSRIs, fluoxetine has the least favourable pharmacokinetic profile and was found to be the antidepressant prescribed most often. Amitriptyline, which was the TCA prescribed most frequently, produces the greatest degree of anticholinergic, sedative and hypotensive effects, relative to other agents in the same antidepressant class. Thus, it is not recommended as a first-line antidepressant, especially in depressed patients with comorbidities. This study identifies potential areas of intervention regarding antidepressant prescribing in depressed individuals with chronic comorbid disease states and offers recommendations to promote the selection of optimal, individualised drug treatment strategies for these patients.
- Full Text:
- Date Issued: 2007
Photodynamic antimicrobial chemotherapy activities of porphyrin- and phthalocyanine-platinum nanoparticle conjugates
- Authors: Managa, Muthumuni Elizabeth
- Date: 2015
- Subjects: Photochemotherapy , Anti-infective agents , Porphyrins , Phthalocyanines , Platinum , Nanoparticles , Bioconjugates , Electrospinning
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4539 , http://hdl.handle.net/10962/d1017919
- Description: This work reports on the conjugation of differently shaped Pt nanoparticles (PtNPs) with ClGa(III) 5,10,15,20-tetrakis-(4-carboxyphenyl) porphyrin (1) as well as chloro - (5,10,15,20-tetrakis (4- (4- carboxy phenycarbonoimidoyl) phenyl) porphyrinato) gallium(III) (2) The work also reports on platination of dihydroxosilicon octacarboxyphthalocyanine (OH)₂SiOCPc (3) to give dihydroxosilicontris(diaquaplatinum)octacarboxyphthalocyanine (OH)₂SiOCPc(Pt)₃ (4). The resulting conjugates were used for photodynamic antimicrobial chemotherapy against S. aureus, E. coli and C. albicans. The degree of photo-inactivation is dependent on concentration of the conjugates, light dose (fluence) and illumination time. The log reduction obtained for 1 when conjugated to cubic PtNPs was 4.64 log (which indicate 99.99 percent of the bacteria have been killed), which is much higher than 3.94 log unit for 1-hexagonal PtNPs and 3.31 log units for 1-unshaped PtNPs. Complex 2 conjugated to hexagonal PtNPs showed 18 nm red shift in the Soret band when compared to 2 alone. Complex 2 and 2-hexagonal PtNPs as well showed promising photodynamic antimicrobial chemotherapy (PACT) activity against S. aureus, E. coli and C. albicans in solution where the log reduction obtained was 4.92, 3.76, and 3.95 respectively for 2-hexagonal PtNPs. The singlet oxygen quantum yields obtained were higher at 0.56 for 2-hexagonl PtNPs in DMF while that of 2 was 0.52 in the same solvent. This resulted in improved PACT activity for 2-hexagonal PtNPs compared to 2. Complex 4 showed slight blue shifting of the absorption spectrum when compared to complex 3 The antimicrobial activity of 4 were promising as the highest log reduction value was observed when compared to the porphyrin conjugates.
- Full Text:
- Date Issued: 2015
- Authors: Managa, Muthumuni Elizabeth
- Date: 2015
- Subjects: Photochemotherapy , Anti-infective agents , Porphyrins , Phthalocyanines , Platinum , Nanoparticles , Bioconjugates , Electrospinning
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4539 , http://hdl.handle.net/10962/d1017919
- Description: This work reports on the conjugation of differently shaped Pt nanoparticles (PtNPs) with ClGa(III) 5,10,15,20-tetrakis-(4-carboxyphenyl) porphyrin (1) as well as chloro - (5,10,15,20-tetrakis (4- (4- carboxy phenycarbonoimidoyl) phenyl) porphyrinato) gallium(III) (2) The work also reports on platination of dihydroxosilicon octacarboxyphthalocyanine (OH)₂SiOCPc (3) to give dihydroxosilicontris(diaquaplatinum)octacarboxyphthalocyanine (OH)₂SiOCPc(Pt)₃ (4). The resulting conjugates were used for photodynamic antimicrobial chemotherapy against S. aureus, E. coli and C. albicans. The degree of photo-inactivation is dependent on concentration of the conjugates, light dose (fluence) and illumination time. The log reduction obtained for 1 when conjugated to cubic PtNPs was 4.64 log (which indicate 99.99 percent of the bacteria have been killed), which is much higher than 3.94 log unit for 1-hexagonal PtNPs and 3.31 log units for 1-unshaped PtNPs. Complex 2 conjugated to hexagonal PtNPs showed 18 nm red shift in the Soret band when compared to 2 alone. Complex 2 and 2-hexagonal PtNPs as well showed promising photodynamic antimicrobial chemotherapy (PACT) activity against S. aureus, E. coli and C. albicans in solution where the log reduction obtained was 4.92, 3.76, and 3.95 respectively for 2-hexagonal PtNPs. The singlet oxygen quantum yields obtained were higher at 0.56 for 2-hexagonl PtNPs in DMF while that of 2 was 0.52 in the same solvent. This resulted in improved PACT activity for 2-hexagonal PtNPs compared to 2. Complex 4 showed slight blue shifting of the absorption spectrum when compared to complex 3 The antimicrobial activity of 4 were promising as the highest log reduction value was observed when compared to the porphyrin conjugates.
- Full Text:
- Date Issued: 2015
Effects of treatment on Lantana camara (L.) and the restoration potential of riparian seed banks in cleared areas of the Victoria Falls World Heritage Site, Livingstone, Zambia
- Nang'alelwa, Michael Mubitelela
- Authors: Nang'alelwa, Michael Mubitelela
- Date: 2010
- Subjects: Lantana camara -- Victoria Falls (Zambia and Zimbabwe) , Lantana camara -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Invasive plants -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Riparian restoration -- Victoria Falls (Zambia and Zimbabwe) , Riparian ecology -- Victoria Falls (Zambia and Zimbabwe)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4217 , http://hdl.handle.net/10962/d1003786 , Lantana camara -- Victoria Falls (Zambia and Zimbabwe) , Lantana camara -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Invasive plants -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Riparian restoration -- Victoria Falls (Zambia and Zimbabwe) , Riparian ecology -- Victoria Falls (Zambia and Zimbabwe)
- Description: The exotic plant Lantana camara L. has invaded the riparian areas of the Victoria Falls World Heritage Site in Livingstone, southern Zambia, threatening native plant communities which support populations of species of special concern. I trialled the mechanical control method of manual uprooting and 3 different herbicides applied through paint brushing of an imazapyr concentrate at 250g. l¯¹, spraying on cut stumps with metsulfron methyl at 600g.l¯¹, and foliar spraying on re-emergent lantana foliage with glyphosate at a dosage of 166g. l¯¹ in July 2008 in 20 100m2 treatment plots, 5 invaded control plots and 5 uninvaded controls. Follow-up treatments for re-sprouting lantana stumps and emerging seedlings were undertaken in June 2009. I measured effectiveness of the methods using adult lantana mortality in June 2009 and lantana seedling density in the different treatment plots during the follow-up exercise. The cost of the various methods and human labour applied were compared across the four treatments at initial clear and at follow-up. All treatments recorded a high adult lantana mortality rate, though there were no significant differences in lantana adult mortality amongst the treatments. Overall, uprooting had the highest adult mortality, followed by imazapyr, metsulfron and lastly glyphosate. Germination of lantana seedlings after clearing was high for all treatments but with no significant differences occurring between the treatments. Both adult lantana mortality and seedling density were however significantly different from the control. With labour included, chemical costs were far higher relative to uprooting, though uprooting costs were the highest when it came to the follow-up because of the emerging seedlings and some resprouting stumps. The effects of mechanical and chemical treatments on vegetation composition in the cleared areas were also assessed in order to detect any non-target and medium term effects of treatments. Contrary to expectation, none of the chemicals showed any significant effects on vegetation composition in the short and medium-term and no significant differences were found in plant species richness, diversity and seedling density between invaded and uninvaded plots at baseline, in October 2008 and in September 2009. In order to determine potential for unaided vegetation recovery in the riparian areas of the study site after lantana clearing, I conducted an investigation of soil seed banks and seed rain using 60 seed bank samples measuring 1800m³ collected from 30 invaded and uninvaded plots. Using the seedling emergence method, 1, 991 seedlings belonging to 66 species representing 27 families germinated from the seed bank. Sedges (Cyperaceae family) were the most abundant taxa in the seed banks from invaded areas, followed by Ageratum conyzoides, lantana, Triumfetta annua and Achyranthes aspera which also occurred in the uninvaded soil seed banks. The seed banks from uninvaded plots were dominated by the grass Oplismenus hirtellus. Overall, species richness, diversity and seedling density from seed banks in invaded areas did not differ significantly from seed bank in uninvaded areas and there was a low similarity in species composition when above ground vegetation was compared to seed banks from invaded and uninvaded areas. It would appear if natural regeneration occured from the current seed bank in disturbed areas, future vegetation would largely comprise of short lived, early successional species in the short term as the seed bank is dominated by non-native herbaceous weedy species. From the seed traps investigating seed rain, a total of 27 species numbering 623 individual seeds were found in the thirty 1m² seedtraps distributed in invaded and uninvaded areas at the five sites, over an intermittent period of three months. Lantana had the highest monthly arrival rate in the seed traps followed by Phoenix reclinata and Ricinus communis. The number of species with invasive potential found in the seed traps located in invaded areas was more than that found in seed traps under native vegetation cover by far. Considerable forest remnants still occur around the invaded sites, and these could serve as an important source for long-term natural re-establishment of native vegetation if seed availability by animals and wind dispersal continues, while the re-invasion of lantana is prevented by ongoing follow-ups and futher clearing of lantana invaded areas. It is concluded that while uprooting and other treatments are effective in the control of lantana, its successful control in the Victoria Falls World Heritage Site will require extensive clearing to keep it from reinvading infested areas after clearing as shown by the seed rain data. The high seedling density of lantana in the seed banks and in the cleared areas shows the need for ongoing follow-up in order to deplete soil stored seed banks. There is need for longer term research to establish what the exact follow-up requirements are in order to contain lantana re-infestation and create favourable micro-sites for native species to establish. It is predicted that ongoing lantana control in the cleared plots will most likely initiate long-term community recovery.
- Full Text:
- Date Issued: 2010
- Authors: Nang'alelwa, Michael Mubitelela
- Date: 2010
- Subjects: Lantana camara -- Victoria Falls (Zambia and Zimbabwe) , Lantana camara -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Invasive plants -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Riparian restoration -- Victoria Falls (Zambia and Zimbabwe) , Riparian ecology -- Victoria Falls (Zambia and Zimbabwe)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4217 , http://hdl.handle.net/10962/d1003786 , Lantana camara -- Victoria Falls (Zambia and Zimbabwe) , Lantana camara -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Invasive plants -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Riparian restoration -- Victoria Falls (Zambia and Zimbabwe) , Riparian ecology -- Victoria Falls (Zambia and Zimbabwe)
- Description: The exotic plant Lantana camara L. has invaded the riparian areas of the Victoria Falls World Heritage Site in Livingstone, southern Zambia, threatening native plant communities which support populations of species of special concern. I trialled the mechanical control method of manual uprooting and 3 different herbicides applied through paint brushing of an imazapyr concentrate at 250g. l¯¹, spraying on cut stumps with metsulfron methyl at 600g.l¯¹, and foliar spraying on re-emergent lantana foliage with glyphosate at a dosage of 166g. l¯¹ in July 2008 in 20 100m2 treatment plots, 5 invaded control plots and 5 uninvaded controls. Follow-up treatments for re-sprouting lantana stumps and emerging seedlings were undertaken in June 2009. I measured effectiveness of the methods using adult lantana mortality in June 2009 and lantana seedling density in the different treatment plots during the follow-up exercise. The cost of the various methods and human labour applied were compared across the four treatments at initial clear and at follow-up. All treatments recorded a high adult lantana mortality rate, though there were no significant differences in lantana adult mortality amongst the treatments. Overall, uprooting had the highest adult mortality, followed by imazapyr, metsulfron and lastly glyphosate. Germination of lantana seedlings after clearing was high for all treatments but with no significant differences occurring between the treatments. Both adult lantana mortality and seedling density were however significantly different from the control. With labour included, chemical costs were far higher relative to uprooting, though uprooting costs were the highest when it came to the follow-up because of the emerging seedlings and some resprouting stumps. The effects of mechanical and chemical treatments on vegetation composition in the cleared areas were also assessed in order to detect any non-target and medium term effects of treatments. Contrary to expectation, none of the chemicals showed any significant effects on vegetation composition in the short and medium-term and no significant differences were found in plant species richness, diversity and seedling density between invaded and uninvaded plots at baseline, in October 2008 and in September 2009. In order to determine potential for unaided vegetation recovery in the riparian areas of the study site after lantana clearing, I conducted an investigation of soil seed banks and seed rain using 60 seed bank samples measuring 1800m³ collected from 30 invaded and uninvaded plots. Using the seedling emergence method, 1, 991 seedlings belonging to 66 species representing 27 families germinated from the seed bank. Sedges (Cyperaceae family) were the most abundant taxa in the seed banks from invaded areas, followed by Ageratum conyzoides, lantana, Triumfetta annua and Achyranthes aspera which also occurred in the uninvaded soil seed banks. The seed banks from uninvaded plots were dominated by the grass Oplismenus hirtellus. Overall, species richness, diversity and seedling density from seed banks in invaded areas did not differ significantly from seed bank in uninvaded areas and there was a low similarity in species composition when above ground vegetation was compared to seed banks from invaded and uninvaded areas. It would appear if natural regeneration occured from the current seed bank in disturbed areas, future vegetation would largely comprise of short lived, early successional species in the short term as the seed bank is dominated by non-native herbaceous weedy species. From the seed traps investigating seed rain, a total of 27 species numbering 623 individual seeds were found in the thirty 1m² seedtraps distributed in invaded and uninvaded areas at the five sites, over an intermittent period of three months. Lantana had the highest monthly arrival rate in the seed traps followed by Phoenix reclinata and Ricinus communis. The number of species with invasive potential found in the seed traps located in invaded areas was more than that found in seed traps under native vegetation cover by far. Considerable forest remnants still occur around the invaded sites, and these could serve as an important source for long-term natural re-establishment of native vegetation if seed availability by animals and wind dispersal continues, while the re-invasion of lantana is prevented by ongoing follow-ups and futher clearing of lantana invaded areas. It is concluded that while uprooting and other treatments are effective in the control of lantana, its successful control in the Victoria Falls World Heritage Site will require extensive clearing to keep it from reinvading infested areas after clearing as shown by the seed rain data. The high seedling density of lantana in the seed banks and in the cleared areas shows the need for ongoing follow-up in order to deplete soil stored seed banks. There is need for longer term research to establish what the exact follow-up requirements are in order to contain lantana re-infestation and create favourable micro-sites for native species to establish. It is predicted that ongoing lantana control in the cleared plots will most likely initiate long-term community recovery.
- Full Text:
- Date Issued: 2010
Deploying DNSSEC in islands of security
- Authors: Murisa, Wesley Vengayi
- Date: 2013 , 2013-03-31
- Subjects: Internet domain names , Computer security , Computer network protocols , Computer security -- Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4577 , http://hdl.handle.net/10962/d1003053 , Internet domain names , Computer security , Computer network protocols , Computer security -- Africa
- Description: The Domain Name System (DNS), a name resolution protocol is one of the vulnerable network protocols that has been subjected to many security attacks such as cache poisoning, denial of service and the 'Kaminsky' spoofing attack. When DNS was designed, security was not incorporated into its design. The DNS Security Extensions (DNSSEC) provides security to the name resolution process by using public key cryptosystems. Although DNSSEC has backward compatibility with unsecured zones, it only offers security to clients when communicating with security aware zones. Widespread deployment of DNSSEC is therefore necessary to secure the name resolution process and provide security to the Internet. Only a few Top Level Domains (TLD's) have deployed DNSSEC, this inherently makes it difficult for their sub-domains to implement the security extensions to the DNS. This study analyses mechanisms that can be used by domains in islands of security to deploy DNSSEC so that the name resolution process can be secured in two specific cases where either the TLD is not signed or the domain registrar is not able to support signed domains. The DNS client side mechanisms evaluated in this study include web browser plug-ins, local validating resolvers and domain look-aside validation. The results of the study show that web browser plug-ins cannot work on their own without local validating resolvers. The web browser validators, however, proved to be useful in indicating to the user whether a domain has been validated or not. Local resolvers present a more secure option for Internet users who cannot trust the communication channel between their stub resolvers and remote name servers. However, they do not provide a way of showing the user whether a domain name has been correctly validated or not. Based on the results of the tests conducted, it is recommended that local validators be used with browser validators for visibility and improved security. On the DNS server side, Domain Look-aside Validation (DLV) presents a viable alternative for organizations in islands of security like most countries in Africa where only two country code Top Level Domains (ccTLD) have deployed DNSSEC. This research recommends use of DLV by corporates to provide DNS security to both internal and external users accessing their web based services. , LaTeX with hyperref package , pdfTeX-1.40.10
- Full Text:
- Date Issued: 2013
- Authors: Murisa, Wesley Vengayi
- Date: 2013 , 2013-03-31
- Subjects: Internet domain names , Computer security , Computer network protocols , Computer security -- Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4577 , http://hdl.handle.net/10962/d1003053 , Internet domain names , Computer security , Computer network protocols , Computer security -- Africa
- Description: The Domain Name System (DNS), a name resolution protocol is one of the vulnerable network protocols that has been subjected to many security attacks such as cache poisoning, denial of service and the 'Kaminsky' spoofing attack. When DNS was designed, security was not incorporated into its design. The DNS Security Extensions (DNSSEC) provides security to the name resolution process by using public key cryptosystems. Although DNSSEC has backward compatibility with unsecured zones, it only offers security to clients when communicating with security aware zones. Widespread deployment of DNSSEC is therefore necessary to secure the name resolution process and provide security to the Internet. Only a few Top Level Domains (TLD's) have deployed DNSSEC, this inherently makes it difficult for their sub-domains to implement the security extensions to the DNS. This study analyses mechanisms that can be used by domains in islands of security to deploy DNSSEC so that the name resolution process can be secured in two specific cases where either the TLD is not signed or the domain registrar is not able to support signed domains. The DNS client side mechanisms evaluated in this study include web browser plug-ins, local validating resolvers and domain look-aside validation. The results of the study show that web browser plug-ins cannot work on their own without local validating resolvers. The web browser validators, however, proved to be useful in indicating to the user whether a domain has been validated or not. Local resolvers present a more secure option for Internet users who cannot trust the communication channel between their stub resolvers and remote name servers. However, they do not provide a way of showing the user whether a domain name has been correctly validated or not. Based on the results of the tests conducted, it is recommended that local validators be used with browser validators for visibility and improved security. On the DNS server side, Domain Look-aside Validation (DLV) presents a viable alternative for organizations in islands of security like most countries in Africa where only two country code Top Level Domains (ccTLD) have deployed DNSSEC. This research recommends use of DLV by corporates to provide DNS security to both internal and external users accessing their web based services. , LaTeX with hyperref package , pdfTeX-1.40.10
- Full Text:
- Date Issued: 2013
An investigation of the polarization of solar radio noise
- Authors: Verschuur, Gerrit L.,1937-
- Date: 1961
- Subjects: Solar radio emission , Polarimetry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5547 , http://hdl.handle.net/10962/d1013441
- Description: CHAPTER I A description of the sun and the type of radio radiation it emits is given. The relation that exists between this and other events occurring on the sun's surface is studied. CHAPTER II The literature dealing with the origin of solar radio noise is reviewed. CHAPTER III The method of specifying polarized radiation and the effect of a magneto- ionic medium on such radiation is discussed. The possible origin of the polarization of solar radio noise is examined and the literature relating to this and to the observations of polarization of solar noise is reviewed. A short outline of the methods used in measuring polarization is given. CHAPTER IV A detailed outline of the construction of a polarimeter is given together with full circuit diagrams and illustrative photographs. CHAPTER V A brief discussion of the operation of the polarimeter, the results obtained and suggestions for its future operation is given.
- Full Text:
- Date Issued: 1961
- Authors: Verschuur, Gerrit L.,1937-
- Date: 1961
- Subjects: Solar radio emission , Polarimetry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5547 , http://hdl.handle.net/10962/d1013441
- Description: CHAPTER I A description of the sun and the type of radio radiation it emits is given. The relation that exists between this and other events occurring on the sun's surface is studied. CHAPTER II The literature dealing with the origin of solar radio noise is reviewed. CHAPTER III The method of specifying polarized radiation and the effect of a magneto- ionic medium on such radiation is discussed. The possible origin of the polarization of solar radio noise is examined and the literature relating to this and to the observations of polarization of solar noise is reviewed. A short outline of the methods used in measuring polarization is given. CHAPTER IV A detailed outline of the construction of a polarimeter is given together with full circuit diagrams and illustrative photographs. CHAPTER V A brief discussion of the operation of the polarimeter, the results obtained and suggestions for its future operation is given.
- Full Text:
- Date Issued: 1961
The mode of action of the acyl-diethylamide molluscicides
- Authors: Solomon, Keith Ross
- Date: 1971
- Subjects: Pesticides , Molluscicides
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5821 , http://hdl.handle.net/10962/d1007406
- Description: From Introduction: That bilharziasis an important public health, social and economic problem in the tropical and subtropical countries of the world is well appreciated. (WHO, 1965) Bilharsiasis is the disease caused by parasitic worms of the genus Schistosoma having certain fresh water snails as the intermediate host. In South Africa the more important snails are Bulinus africanus and B. globosus, the secondary hosts of Schistosom haematobium. (WHO, 1965) One method of controling the disease is to reduce the numbers of the intermediate host snails to such a low level that transmission cannot occur (WHO, 1965 and Macdonald, 1965) This has led to a strong interest in those substances that kill snails. The first recorded use of a molluscicide, copper sulphate, was during the first world War in Egypt. (De Villiers, 1965) More recently, the more or less random testing of large numbers of compounds led to the discovery of a large number of molluscicides. These were the heavy metal compounds such as the salts of mercury and arsenic, the substituted phenols such as pentachlorophenol (PCP) and the 2-substituted 4:6-dinitrophenols. (De Villiers, 1965) These compounds are good molluscicides but suffer from a number of disadvantages. The use of copper compounds in hard waters is impractical as the copper precipitates out as the less effective carbonate. The heavy metal salts are expensive and biocidal thus making the treated water unsuitable for human or agricultural use. The phenolic compounds are unpleasant and dangerous to handle and the hydrolysis of PCP is catalysed by sunlight in clear waters. High concentrations of these compounds are required, thus increasing transport and handling costs, particularly in inaccessible areas.
- Full Text:
- Date Issued: 1971
- Authors: Solomon, Keith Ross
- Date: 1971
- Subjects: Pesticides , Molluscicides
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5821 , http://hdl.handle.net/10962/d1007406
- Description: From Introduction: That bilharziasis an important public health, social and economic problem in the tropical and subtropical countries of the world is well appreciated. (WHO, 1965) Bilharsiasis is the disease caused by parasitic worms of the genus Schistosoma having certain fresh water snails as the intermediate host. In South Africa the more important snails are Bulinus africanus and B. globosus, the secondary hosts of Schistosom haematobium. (WHO, 1965) One method of controling the disease is to reduce the numbers of the intermediate host snails to such a low level that transmission cannot occur (WHO, 1965 and Macdonald, 1965) This has led to a strong interest in those substances that kill snails. The first recorded use of a molluscicide, copper sulphate, was during the first world War in Egypt. (De Villiers, 1965) More recently, the more or less random testing of large numbers of compounds led to the discovery of a large number of molluscicides. These were the heavy metal compounds such as the salts of mercury and arsenic, the substituted phenols such as pentachlorophenol (PCP) and the 2-substituted 4:6-dinitrophenols. (De Villiers, 1965) These compounds are good molluscicides but suffer from a number of disadvantages. The use of copper compounds in hard waters is impractical as the copper precipitates out as the less effective carbonate. The heavy metal salts are expensive and biocidal thus making the treated water unsuitable for human or agricultural use. The phenolic compounds are unpleasant and dangerous to handle and the hydrolysis of PCP is catalysed by sunlight in clear waters. High concentrations of these compounds are required, thus increasing transport and handling costs, particularly in inaccessible areas.
- Full Text:
- Date Issued: 1971
Characterization of chromatic dispersion in single mode fibre
- Authors: Wassin, Shukree
- Date: 2014
- Subjects: Fiber optics Electromagnetic waves , Electromagnetic waves -- Transmission
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/47807 , vital:40379
- Description: In this dissertation, an investigation of chromatic dispersion is presented. The Pulse delay and phase Shift chromatic dispersion characterization techniques were used for conducting the measurements. The experiments were performed in a modern optical fibre research laboratory and chromatic dispersion measurements were carried out on several lengths of G.652 and G.655 single mode fibres. The pulse delay characterization technique measures the time of flight between two modulated optical signals whilst propagating along the fibre under test. During phase shift experiments, the group delay is obtained by measuring the relative phase difference as a function of wavelength, between adjacent sinusoidal light signals. The pulse delay and phase shift characterization techniques illustrated excellent agreement in the measured! chromatic Dispersion coefficients along the G.652 standard single mode! fibre as well as the G.655 positive and negative non-zero dispersion shifted fibre. It was found that the measurement accuracy improved as the fibre length increased. A periodic shift between the modulated optical signals, propagating along the fibre was experimentally observed. It is to be remarked that the longer wavelength signals propagated faster along the G.655 positive non-zero dispersion shifted fibre in comparison to its transmission within the G.655 negative non-zero dispersion shifted fibre. Furthermore, it was found that the sinusoidal signal shifted towards the left along the G.655 negative NZDSF fibre whilst the shift occurred towards the right along the G.655 positive NZDSF fibre. Generally, the shift arising along the G.655 fibres was found to be smaller than the shift seen throughout the G.652 fibres. Towards the end of this study, a chromatic dispersion compensation system was designed and tested. Once characterization of the compensation link was completed, it was experimentally illustrated that the chromatic dispersion across the system was successfully reduced. Finally, a sum of squares of error statistical test showed that the phase shift technique is more accurate in comparison to the pulse delay method. This result was found to be in good agreement with published work found in literature.
- Full Text:
- Date Issued: 2014
- Authors: Wassin, Shukree
- Date: 2014
- Subjects: Fiber optics Electromagnetic waves , Electromagnetic waves -- Transmission
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/47807 , vital:40379
- Description: In this dissertation, an investigation of chromatic dispersion is presented. The Pulse delay and phase Shift chromatic dispersion characterization techniques were used for conducting the measurements. The experiments were performed in a modern optical fibre research laboratory and chromatic dispersion measurements were carried out on several lengths of G.652 and G.655 single mode fibres. The pulse delay characterization technique measures the time of flight between two modulated optical signals whilst propagating along the fibre under test. During phase shift experiments, the group delay is obtained by measuring the relative phase difference as a function of wavelength, between adjacent sinusoidal light signals. The pulse delay and phase shift characterization techniques illustrated excellent agreement in the measured! chromatic Dispersion coefficients along the G.652 standard single mode! fibre as well as the G.655 positive and negative non-zero dispersion shifted fibre. It was found that the measurement accuracy improved as the fibre length increased. A periodic shift between the modulated optical signals, propagating along the fibre was experimentally observed. It is to be remarked that the longer wavelength signals propagated faster along the G.655 positive non-zero dispersion shifted fibre in comparison to its transmission within the G.655 negative non-zero dispersion shifted fibre. Furthermore, it was found that the sinusoidal signal shifted towards the left along the G.655 negative NZDSF fibre whilst the shift occurred towards the right along the G.655 positive NZDSF fibre. Generally, the shift arising along the G.655 fibres was found to be smaller than the shift seen throughout the G.652 fibres. Towards the end of this study, a chromatic dispersion compensation system was designed and tested. Once characterization of the compensation link was completed, it was experimentally illustrated that the chromatic dispersion across the system was successfully reduced. Finally, a sum of squares of error statistical test showed that the phase shift technique is more accurate in comparison to the pulse delay method. This result was found to be in good agreement with published work found in literature.
- Full Text:
- Date Issued: 2014
Template-assisted sol-gel synthesis of carbon doped titanium dioxide nanotubes and their characterization
- Authors: Takata, Nwabisa
- Date: 2017
- Subjects: Nanotechnology Titanium dioxide Nanostructures
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/13194 , vital:39473
- Description: This study reveals the effects of doping on the morphological, structural and optical properties of titanium dioxide (TiO2) nanotubes (TNTs), synthesized by sol-gel template-assisted sol-gel technique. The nanotubes (TNTs) were prepared in anodic alumina membranes (AAM) with a pore diameter range of 110-210 nm by using titanium tetra butoxide as a sol-gel precursor and oxalic acid dihydrate as a dopant source. The synthesized nanotubes were evaluated using scanning electron microscope (SEM), energy dispersive spectroscopy (EDX), fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), confocal Raman spectroscopy (CRS) and photoluminescence spectroscopy (PL). SEM analysis has revealed the presence of closely-packed TNTs, with a modal external tube diameters of 150, 170, 210,190 and 210 nm for the un-doped TNTs, 9 mM carbon doped-TNTs (C-TNTs), 27 mM C-TNTs, 45 mM C-TNTs and 75 mM C-TNTs respectively. The diameters are consistent with the AAM diameter range. EDX spectra revealed the presence of Ti peaks at 0.45 and 4.9 keV corresponding to Kα1 and Kβ1 emission lines respectively. Oxygen exhibits a signal at 0.5 keV corresponding to Kα1 emission line. The occurrence of these peaks in the EDX spectra endorses the existence of Ti and O atoms in the prepared titanium dioxide nanotubes. FTIR spectroscopy has revealed the presence of vibration modes at 580-660 cm-1 indicating the presence of Ti-O bonds and additional vibration modes at 2324 cm-1 resulting from C-O stretching in the C-TNTs. The XRD analysis has revealed the presence of a mixed anatase-brookite phase with diffraction peaks at 2θ angles of 25.49⁰, 38.11⁰, 40.60º, 48.14⁰, 54.58⁰, 63.00⁰, 70.11⁰ and 75.66⁰. Additionally, XRD analysis has revealed elongation of lattice parameter “c” from 9.143 to 9.830 Å with carbon concentration increase. Lattice expansion indicates the possibility of carbon substituting oxygen sites. Raman large area scan has revealed the presence of rutile, brookite and anatase for the undoped samples. On doping the rutile phase of TiO2 has shown to be suppressed by the presence of carbon atoms such that the doped samples consist of brookite and anatase phases only. The Eg1 mode of anatase of the undoped TNTs at 153. 78 cm-1 was red shifted by Δ9.78 cm-1 relative to the bulk anatase TiO2. This was attributed to decrease in particle size, presence of brookite and phonon confinement. Upon doping, the 9 mM C-TNTs, 27 mM TNTs and 75 mM TNTs have shown a red shift of Δ0. 09 cm-1, Δ1. 39 cm-1 and 1.81 cm-1 respectively, suggesting the incorporation of carbon in the TiO2 matrix. CRS depth profiling in the XZ direction has also validated the presence of a mixed anatase-brookite phase at Raman active modes 153.19 cm-1, 208.87 cm-1, 404.55 cm-1, 523.26 cm-1 and 648.55 cm-1. Photoluminescence spectra of carbon doped TiO2 showed two emission peaks at 398 nm attributed to annihilation of excitons while the broad peak at 400-460 nm was attributed to the presence of oxygen vacancies. The peak intensity of the 45 mM C-TNTs has shown a lower PL intensity suggesting that efficiency of charge separation was higher and recombination rate was lower than other carbon containing samples.
- Full Text:
- Date Issued: 2017
- Authors: Takata, Nwabisa
- Date: 2017
- Subjects: Nanotechnology Titanium dioxide Nanostructures
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/13194 , vital:39473
- Description: This study reveals the effects of doping on the morphological, structural and optical properties of titanium dioxide (TiO2) nanotubes (TNTs), synthesized by sol-gel template-assisted sol-gel technique. The nanotubes (TNTs) were prepared in anodic alumina membranes (AAM) with a pore diameter range of 110-210 nm by using titanium tetra butoxide as a sol-gel precursor and oxalic acid dihydrate as a dopant source. The synthesized nanotubes were evaluated using scanning electron microscope (SEM), energy dispersive spectroscopy (EDX), fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), confocal Raman spectroscopy (CRS) and photoluminescence spectroscopy (PL). SEM analysis has revealed the presence of closely-packed TNTs, with a modal external tube diameters of 150, 170, 210,190 and 210 nm for the un-doped TNTs, 9 mM carbon doped-TNTs (C-TNTs), 27 mM C-TNTs, 45 mM C-TNTs and 75 mM C-TNTs respectively. The diameters are consistent with the AAM diameter range. EDX spectra revealed the presence of Ti peaks at 0.45 and 4.9 keV corresponding to Kα1 and Kβ1 emission lines respectively. Oxygen exhibits a signal at 0.5 keV corresponding to Kα1 emission line. The occurrence of these peaks in the EDX spectra endorses the existence of Ti and O atoms in the prepared titanium dioxide nanotubes. FTIR spectroscopy has revealed the presence of vibration modes at 580-660 cm-1 indicating the presence of Ti-O bonds and additional vibration modes at 2324 cm-1 resulting from C-O stretching in the C-TNTs. The XRD analysis has revealed the presence of a mixed anatase-brookite phase with diffraction peaks at 2θ angles of 25.49⁰, 38.11⁰, 40.60º, 48.14⁰, 54.58⁰, 63.00⁰, 70.11⁰ and 75.66⁰. Additionally, XRD analysis has revealed elongation of lattice parameter “c” from 9.143 to 9.830 Å with carbon concentration increase. Lattice expansion indicates the possibility of carbon substituting oxygen sites. Raman large area scan has revealed the presence of rutile, brookite and anatase for the undoped samples. On doping the rutile phase of TiO2 has shown to be suppressed by the presence of carbon atoms such that the doped samples consist of brookite and anatase phases only. The Eg1 mode of anatase of the undoped TNTs at 153. 78 cm-1 was red shifted by Δ9.78 cm-1 relative to the bulk anatase TiO2. This was attributed to decrease in particle size, presence of brookite and phonon confinement. Upon doping, the 9 mM C-TNTs, 27 mM TNTs and 75 mM TNTs have shown a red shift of Δ0. 09 cm-1, Δ1. 39 cm-1 and 1.81 cm-1 respectively, suggesting the incorporation of carbon in the TiO2 matrix. CRS depth profiling in the XZ direction has also validated the presence of a mixed anatase-brookite phase at Raman active modes 153.19 cm-1, 208.87 cm-1, 404.55 cm-1, 523.26 cm-1 and 648.55 cm-1. Photoluminescence spectra of carbon doped TiO2 showed two emission peaks at 398 nm attributed to annihilation of excitons while the broad peak at 400-460 nm was attributed to the presence of oxygen vacancies. The peak intensity of the 45 mM C-TNTs has shown a lower PL intensity suggesting that efficiency of charge separation was higher and recombination rate was lower than other carbon containing samples.
- Full Text:
- Date Issued: 2017
Photosynthetic and growth responses of C3 and C4 grasses to short- duration sunflecks and resultant consequences for their performance in understory environments
- Authors: Adams, Claire Elizabeth
- Date: 2017
- Subjects: Understory plants -- Effect of light on , Grasses -- Effect of light on -- South Africa , Erharta -- Effect of light on , Brachiara -- Effect of light on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/5016 , vital:20753
- Description: Low C4 grass species abundance in understory environments is thought to be as a result of their high-light requirements, lack of photosynthetic advantage relative to C3 species in cooler environments, and an inability to adequately utilise sunflecks. This study sets out to investigate this theory, hypothesizing that C3 grass species outperform C4 grass species under the canopy, not as a result of quantum efficiency temperature effects, but as a result of C4 species inability to utilize short-duration sunflecks. Short sunflecks could result in a breakdown in assimilate movement between the mesophyll (MSC) and bundle sheath (BSC) cells. The role of BSC leakiness, stomata and PSII efficiency on the ability of C3 and C4 Alloteropsis semialata to utilize short-duration sunflecks was investigated using gaseous exchange and chlorophyll fluorescence techniques, while the growth of both subspecies under a simulated flecking-light environment tested whether these measured responses translated into effects on growth. As C3 grasses are known to possess higher levels of stomatal conductance in relation to C4 species, results showed that C3 A. semialata was able to utilize short-duration sunflecks as a result of increased stomatal conductance and an ability to induce photosynthesis under various light flecking conditions. In contrast, C4 A. semialata was unable to utilize sunflecks possibly as a result of energetic limitations of the carboxylation mechanism (PSII) and not because of increased bundle sheath leakiness. These photosynthetic responses translated into growth differences when both types were grown in an artificially flecking light environment. The photosynthetic differences noted for C3 and C4 A.semialata were also evident in shade adapted understory grasses; Erharta erecta (C3), Dactylotenium australe (C4) and Brachiaria chusqueoides (C4). As photosynthetic induction was marginally more rapid in all species relative to A. semialata it suggests some degree of adaptation in shade grasses, however the inability of the C4 species to utilise short sunflecks remained. As a result, it was hypothesized that C3 shade adapted E. erecta and C4 B. chusqueoides, co-inhabiting the same forest understory, are able to do so because of differences in light micro-environments associated with each species. However, canopy openness and light profiles determined for theses micro-climates showed no differences and that both species have persisted within a relatively stable understory environment. The coexistence was possible as the understory was supplied with a significant proportion of its daily light in flecks sufficiently long as to not compromise C4 productivity. A survey of 10 species of grasses under various canopy densities and in the open showed a strong negative linear relationship between canopy openness and the rate at which photosynthesis was induced by flecking light, which has not been shown before. This did not result from the phylogenetic relationship between species and could be shown for a single species (E. erecta) growing in a range of light environments. This demonstrates that C4 grasses, despite adaptation, would be limited from sunfleck environments if a significant proportion of the daily light available consists of flecks of short-duration. The generation of such environments may occur as a result of woody thickening and could help explain the observed decline in C4 grasses under these conditions.
- Full Text:
- Date Issued: 2017
- Authors: Adams, Claire Elizabeth
- Date: 2017
- Subjects: Understory plants -- Effect of light on , Grasses -- Effect of light on -- South Africa , Erharta -- Effect of light on , Brachiara -- Effect of light on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/5016 , vital:20753
- Description: Low C4 grass species abundance in understory environments is thought to be as a result of their high-light requirements, lack of photosynthetic advantage relative to C3 species in cooler environments, and an inability to adequately utilise sunflecks. This study sets out to investigate this theory, hypothesizing that C3 grass species outperform C4 grass species under the canopy, not as a result of quantum efficiency temperature effects, but as a result of C4 species inability to utilize short-duration sunflecks. Short sunflecks could result in a breakdown in assimilate movement between the mesophyll (MSC) and bundle sheath (BSC) cells. The role of BSC leakiness, stomata and PSII efficiency on the ability of C3 and C4 Alloteropsis semialata to utilize short-duration sunflecks was investigated using gaseous exchange and chlorophyll fluorescence techniques, while the growth of both subspecies under a simulated flecking-light environment tested whether these measured responses translated into effects on growth. As C3 grasses are known to possess higher levels of stomatal conductance in relation to C4 species, results showed that C3 A. semialata was able to utilize short-duration sunflecks as a result of increased stomatal conductance and an ability to induce photosynthesis under various light flecking conditions. In contrast, C4 A. semialata was unable to utilize sunflecks possibly as a result of energetic limitations of the carboxylation mechanism (PSII) and not because of increased bundle sheath leakiness. These photosynthetic responses translated into growth differences when both types were grown in an artificially flecking light environment. The photosynthetic differences noted for C3 and C4 A.semialata were also evident in shade adapted understory grasses; Erharta erecta (C3), Dactylotenium australe (C4) and Brachiaria chusqueoides (C4). As photosynthetic induction was marginally more rapid in all species relative to A. semialata it suggests some degree of adaptation in shade grasses, however the inability of the C4 species to utilise short sunflecks remained. As a result, it was hypothesized that C3 shade adapted E. erecta and C4 B. chusqueoides, co-inhabiting the same forest understory, are able to do so because of differences in light micro-environments associated with each species. However, canopy openness and light profiles determined for theses micro-climates showed no differences and that both species have persisted within a relatively stable understory environment. The coexistence was possible as the understory was supplied with a significant proportion of its daily light in flecks sufficiently long as to not compromise C4 productivity. A survey of 10 species of grasses under various canopy densities and in the open showed a strong negative linear relationship between canopy openness and the rate at which photosynthesis was induced by flecking light, which has not been shown before. This did not result from the phylogenetic relationship between species and could be shown for a single species (E. erecta) growing in a range of light environments. This demonstrates that C4 grasses, despite adaptation, would be limited from sunfleck environments if a significant proportion of the daily light available consists of flecks of short-duration. The generation of such environments may occur as a result of woody thickening and could help explain the observed decline in C4 grasses under these conditions.
- Full Text:
- Date Issued: 2017
The effect of appetite suppressants on pineal function
- Authors: Mchunu, Bongani Isaac
- Date: 1994
- Subjects: Pineal gland -- Research , Pineal gland -- Secretions , Appetite depressants -- Physiological effect
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4038 , http://hdl.handle.net/10962/d1004098 , Pineal gland -- Research , Pineal gland -- Secretions , Appetite depressants -- Physiological effect
- Description: The pineal gland has become the subject of considerable investigation as it provides a productive experimental model for studying circadian rhythms and regulation of end organs. In the rat, the pineal gland provides a convenient model for investigating the noradrenergic receptor system and the effects of various drugs on this system. The effect of appetite suppressants on the rat pineal gland function is described. Appetite suppressants increase melatonin synthesis in organ cultures of rat pineal glands. This effect appears to be mediated by noradrenaline acting on β-adrenoceptors on the pinealocyte membrane. When β-adrenoceptors are blocked, the appetite suppressant-induced rise in melatonin synthesis is prevented. Depletion of noradrenaline in sympathetic nerve terminals also prevented the appetite suppressant-induced rise in melatonin synthesis. Activation of β-adrenoceptors is followed by a rise in N-acetyltransferase activity via a cyclic adenosine monophosphate second messenger system. The effect of appetite suppressants on the activity of liver tryptophan pyrrolase was also investigated. The activity of this enzyme is an important determinant of tryptophan availability to the brain and consequently of brain serotonin levels. The results show that appetite suppressants inhibit both holoenzyme and total enzyme activities of tryptophan pyrrolase. This finding suggests that appetite suppressants may act by inhibiting tryptophan pyrrolase activity thereby increasing brain serotonin, a phenomenon known to be associated with anorexia. There are two possible mechanisms by which appetite suppressants inhibit tryptophan pyrrolase activity. Firstly, these agents, being drugs of dependence, may increase liver NADPH concentrations which inhibit pyrrolase activity. Secondly, appetite suppressants may act on the pineal gland to stimulate melatonin synthesis. Melatonin inhibits pyrrolase activity in a dose-dependent manner. This inhibition will elevate plasma tryptophan levels which result in a rise in brain serotonin synthesis. The present study suggests a possible relationship between the pineal gland and appetite centres in the hypothalamus. Melatonin may have a direct effect on appetite centres since food restriction is associated with an increased melatonin binding in the hypothalamus. If this possible relationship can be extended, melatonin can open new possibilities for the control of food intake and consequently, of pathological obesity.
- Full Text:
- Date Issued: 1994
- Authors: Mchunu, Bongani Isaac
- Date: 1994
- Subjects: Pineal gland -- Research , Pineal gland -- Secretions , Appetite depressants -- Physiological effect
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4038 , http://hdl.handle.net/10962/d1004098 , Pineal gland -- Research , Pineal gland -- Secretions , Appetite depressants -- Physiological effect
- Description: The pineal gland has become the subject of considerable investigation as it provides a productive experimental model for studying circadian rhythms and regulation of end organs. In the rat, the pineal gland provides a convenient model for investigating the noradrenergic receptor system and the effects of various drugs on this system. The effect of appetite suppressants on the rat pineal gland function is described. Appetite suppressants increase melatonin synthesis in organ cultures of rat pineal glands. This effect appears to be mediated by noradrenaline acting on β-adrenoceptors on the pinealocyte membrane. When β-adrenoceptors are blocked, the appetite suppressant-induced rise in melatonin synthesis is prevented. Depletion of noradrenaline in sympathetic nerve terminals also prevented the appetite suppressant-induced rise in melatonin synthesis. Activation of β-adrenoceptors is followed by a rise in N-acetyltransferase activity via a cyclic adenosine monophosphate second messenger system. The effect of appetite suppressants on the activity of liver tryptophan pyrrolase was also investigated. The activity of this enzyme is an important determinant of tryptophan availability to the brain and consequently of brain serotonin levels. The results show that appetite suppressants inhibit both holoenzyme and total enzyme activities of tryptophan pyrrolase. This finding suggests that appetite suppressants may act by inhibiting tryptophan pyrrolase activity thereby increasing brain serotonin, a phenomenon known to be associated with anorexia. There are two possible mechanisms by which appetite suppressants inhibit tryptophan pyrrolase activity. Firstly, these agents, being drugs of dependence, may increase liver NADPH concentrations which inhibit pyrrolase activity. Secondly, appetite suppressants may act on the pineal gland to stimulate melatonin synthesis. Melatonin inhibits pyrrolase activity in a dose-dependent manner. This inhibition will elevate plasma tryptophan levels which result in a rise in brain serotonin synthesis. The present study suggests a possible relationship between the pineal gland and appetite centres in the hypothalamus. Melatonin may have a direct effect on appetite centres since food restriction is associated with an increased melatonin binding in the hypothalamus. If this possible relationship can be extended, melatonin can open new possibilities for the control of food intake and consequently, of pathological obesity.
- Full Text:
- Date Issued: 1994
The exploration for and possible genesis of, some Archaean granite/gneiss-hosted gold deposits in the Pietersburg granite-greenstone terrane
- Linklater, Michael Anthony Leonard Flanders
- Authors: Linklater, Michael Anthony Leonard Flanders
- Date: 1992
- Subjects: Gold ores -- Geology -- South Africa , Gneiss , Granite
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4945 , http://hdl.handle.net/10962/d1005557 , Gold ores -- Geology -- South Africa , Gneiss , Granite
- Description: Abstract The gold mineralization event within Archaean granite-greenstone terranes occurred during the late Archaean, and followed the intrusion of syn- to late-tectonic granitic plutons into previously deformed greenstone belts. An Archaean granite/gneiss-hosted gold deposit, in terms of this project, is classified as having a gold-assay cutoff of 1g/metric ton over widths of at least several metres, or higher grades over narrower widths and/or verbal descriptions that indicate such values. Fluid inclusion studies and isotopic data identify two possible origins for the auriferous fluids; namely magmatic and metamorphic. The exploration target according to the magmatic model, is a late-Archaean, hydrothermally altered, mineralized and fractured granitic intrusion preferably with a granodioritic or quartz-dioritic composition. The exploration target according to the metamorphic replacement model is a granitic stock that has intruded a zone of crustal weakness such as a shear zone, active during the late Archaean. Alternatively, the granitic intrusion should be affected by regionally extensive late-Archaean shearing. It should be hydrothermally altered, deformed and mineralized. Five areas within the Pietersburg granite-greenstone terrane were selected for the 'Regional Area Selection' phase of exploration for Archaean granite/gneiss-hosted gold deposits; namely Roodepoort, Waterval, Ramagoep, Moletsie and Matlala. Roodepoort contains a known granodiorite-hosted gold deposit; the Knight's Pluton, and served as an orientation survey for this project. The use and interpretation of LANDSAT images formed an integral part of exploration techniques; to assess their usefulness in the exploration of Archaean granite/gneiss-hosted gold deposits. Area selection criteria for granite/gneiss-hosted gold mineralization at Roodepoort are the major ENE-trending shear zone, the NNW-trending lineament and hydrothermal alteration, shearing, quartz-stockworks and sulphide mineralization within the Knight's Pluton. The origin of the gold within the Knight's Pluton is uncertain; both magmatic and metamorphic models are possibilities. Ongoing exploration is in progress at Roodepoort. The only area selection criterion for granite/gneiss-hosted gold mineralization at Waterval is the sericitized, subcropping granites located within trenches. Gold mineralization is insignificant. No area selection criteria for Archaean granite/gneiss-hosted gold mineralization were located at Ramagoep, Matlala and Moletsie. No further exploration is recommended for all these areas. The MES image interpretations were successful in identifying lineaments, granitic outcrops, greenstones, vegetation and soil cover. The Clay-iron images adequately differentiated betweeen iron-rich and clay-bearing areas. However, not all clay-bearing areas were associated with hydrothermal alteratian; field checks were necessary to discriminate between weathered granites and hydrothermally altered granites. The Wallis images served to locally enhance the contrasts of the MES and Clay-iron images.
- Full Text:
- Date Issued: 1992
- Authors: Linklater, Michael Anthony Leonard Flanders
- Date: 1992
- Subjects: Gold ores -- Geology -- South Africa , Gneiss , Granite
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4945 , http://hdl.handle.net/10962/d1005557 , Gold ores -- Geology -- South Africa , Gneiss , Granite
- Description: Abstract The gold mineralization event within Archaean granite-greenstone terranes occurred during the late Archaean, and followed the intrusion of syn- to late-tectonic granitic plutons into previously deformed greenstone belts. An Archaean granite/gneiss-hosted gold deposit, in terms of this project, is classified as having a gold-assay cutoff of 1g/metric ton over widths of at least several metres, or higher grades over narrower widths and/or verbal descriptions that indicate such values. Fluid inclusion studies and isotopic data identify two possible origins for the auriferous fluids; namely magmatic and metamorphic. The exploration target according to the magmatic model, is a late-Archaean, hydrothermally altered, mineralized and fractured granitic intrusion preferably with a granodioritic or quartz-dioritic composition. The exploration target according to the metamorphic replacement model is a granitic stock that has intruded a zone of crustal weakness such as a shear zone, active during the late Archaean. Alternatively, the granitic intrusion should be affected by regionally extensive late-Archaean shearing. It should be hydrothermally altered, deformed and mineralized. Five areas within the Pietersburg granite-greenstone terrane were selected for the 'Regional Area Selection' phase of exploration for Archaean granite/gneiss-hosted gold deposits; namely Roodepoort, Waterval, Ramagoep, Moletsie and Matlala. Roodepoort contains a known granodiorite-hosted gold deposit; the Knight's Pluton, and served as an orientation survey for this project. The use and interpretation of LANDSAT images formed an integral part of exploration techniques; to assess their usefulness in the exploration of Archaean granite/gneiss-hosted gold deposits. Area selection criteria for granite/gneiss-hosted gold mineralization at Roodepoort are the major ENE-trending shear zone, the NNW-trending lineament and hydrothermal alteration, shearing, quartz-stockworks and sulphide mineralization within the Knight's Pluton. The origin of the gold within the Knight's Pluton is uncertain; both magmatic and metamorphic models are possibilities. Ongoing exploration is in progress at Roodepoort. The only area selection criterion for granite/gneiss-hosted gold mineralization at Waterval is the sericitized, subcropping granites located within trenches. Gold mineralization is insignificant. No area selection criteria for Archaean granite/gneiss-hosted gold mineralization were located at Ramagoep, Matlala and Moletsie. No further exploration is recommended for all these areas. The MES image interpretations were successful in identifying lineaments, granitic outcrops, greenstones, vegetation and soil cover. The Clay-iron images adequately differentiated betweeen iron-rich and clay-bearing areas. However, not all clay-bearing areas were associated with hydrothermal alteratian; field checks were necessary to discriminate between weathered granites and hydrothermally altered granites. The Wallis images served to locally enhance the contrasts of the MES and Clay-iron images.
- Full Text:
- Date Issued: 1992
The digestive mechanisms of an intertidal grazer, the sea urchin Parechinus angulosus
- Authors: Sweijd, Neville Anthony
- Date: 1991
- Subjects: Sea urchins -- Nutrition , Sea urchins , Echinoida
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5662 , http://hdl.handle.net/10962/d1005346 , Sea urchins -- Nutrition , Sea urchins , Echinoida
- Description: Echinoids are important grazers in the near-shore ecosystem and can significantly effect their ecology. The sea urchin Parechinus angulosus occurs inter- and subtidally along the southern African coast. Within this range it consumes an extremely wide variety of algae. Since algal cell walls have an almost species specific chemical composition, the question arises as to how it can digest the algae that it consumes. In order to investigate the digestive mechanisms employed by P. angulosus, an ultrastructural study of the gut was undertaken to characterize the tissue and identify functional regions in the gut. Ten structural and storage polysaccharides commonly found in macroalgae were used as substrates to assay the digestive polysaccharidases of the sea urchin. The enteric bacteria of the sea urchin were isolated and tested separately for polysaccharidase activity using the same substrates. The results shown that the gut of Parechinus angulosus is regionally specialized, with the foregut primarily responsible for the production of hydrolytic enzymes, while the hindgut is primarily absorptive. The occurrence of lamellar bodies, heterolysosomes, cytoplasmic blebs and paddle cilia among other characteristic features of the digestive epithelium are described and discussed. Two levels of enzyme activity are apparent. Generally the urchin could hydrolyze the reserve polysaccharides, but only partially hydrolyze the structural polysaccharides, of red and green algae. P.angulosus was unable to digest alginic acid, the main structural polysaccharide of brown algae. Mixed cultures of bacteria utilized only the reserve polysaccharides of red and green algae. Significantly, the bacteria were able to hydrolyze alginic acid. Enteric bacteria also showed agarolytic activity. Parechinus angulosus has the ability to digest red and green algae. No lysozyme activity was detected. The enteric bacteria can digest the same algal reserve polysaccharides and so may compete for carbon in the gut. However, in the case of brown algae, bacteria have a potentially important endosymbiotic role as agents of digestion. These results correspond with food preference studies which have shown that, although P.angulosus consumes the kelp Ecklonia maxima, in the western Cape, it is amongst its least preferred food species. The reasons for this are its unpalatability and the urchin's inability to digest brown algae. The digestibility of algal material can be an important factor in determining algal-herbivore interactions.
- Full Text:
- Date Issued: 1991
- Authors: Sweijd, Neville Anthony
- Date: 1991
- Subjects: Sea urchins -- Nutrition , Sea urchins , Echinoida
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5662 , http://hdl.handle.net/10962/d1005346 , Sea urchins -- Nutrition , Sea urchins , Echinoida
- Description: Echinoids are important grazers in the near-shore ecosystem and can significantly effect their ecology. The sea urchin Parechinus angulosus occurs inter- and subtidally along the southern African coast. Within this range it consumes an extremely wide variety of algae. Since algal cell walls have an almost species specific chemical composition, the question arises as to how it can digest the algae that it consumes. In order to investigate the digestive mechanisms employed by P. angulosus, an ultrastructural study of the gut was undertaken to characterize the tissue and identify functional regions in the gut. Ten structural and storage polysaccharides commonly found in macroalgae were used as substrates to assay the digestive polysaccharidases of the sea urchin. The enteric bacteria of the sea urchin were isolated and tested separately for polysaccharidase activity using the same substrates. The results shown that the gut of Parechinus angulosus is regionally specialized, with the foregut primarily responsible for the production of hydrolytic enzymes, while the hindgut is primarily absorptive. The occurrence of lamellar bodies, heterolysosomes, cytoplasmic blebs and paddle cilia among other characteristic features of the digestive epithelium are described and discussed. Two levels of enzyme activity are apparent. Generally the urchin could hydrolyze the reserve polysaccharides, but only partially hydrolyze the structural polysaccharides, of red and green algae. P.angulosus was unable to digest alginic acid, the main structural polysaccharide of brown algae. Mixed cultures of bacteria utilized only the reserve polysaccharides of red and green algae. Significantly, the bacteria were able to hydrolyze alginic acid. Enteric bacteria also showed agarolytic activity. Parechinus angulosus has the ability to digest red and green algae. No lysozyme activity was detected. The enteric bacteria can digest the same algal reserve polysaccharides and so may compete for carbon in the gut. However, in the case of brown algae, bacteria have a potentially important endosymbiotic role as agents of digestion. These results correspond with food preference studies which have shown that, although P.angulosus consumes the kelp Ecklonia maxima, in the western Cape, it is amongst its least preferred food species. The reasons for this are its unpalatability and the urchin's inability to digest brown algae. The digestibility of algal material can be an important factor in determining algal-herbivore interactions.
- Full Text:
- Date Issued: 1991
An investigation of the role of mitochondrial STAT3 and modulation of Reactive Oxygen Species in adipocyte differentiation
- Authors: Kramer, Adam Hildyard
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/54632 , vital:26595
- Description: Stem cells have the ability to differentiate into a myriad of different cell types. The understanding of the differentiation process is of paramount importance if we are to use these cells in the lab as well as in therapeutics. Here, the levels and localization of the signal transducer and activator of transcription 3 (STAT3), with particular attention focused on the mitochondrial serine 727 phosphorylated form of STAT3 (pSTAT3S727) during differentiation, was investigated. Using the murine preadipocyte progenitor cell line 3T3-L1, as well as adipose derived human mesenchymal stem cells (HMSC-ad) as differentiation models, the relative levels of Reactive Oxygen Species (ROS) and the levels and localization of STAT3 were investigated during the differentiation process. ROS is known to play an important signalling role during differentiation and is well reported during the events of adipogenesis. ROS are generated as a by-product in the Electron Transport Chain (ETC), and it has recently been reported that pSTAT3S727 plays an important role at complex I of the ETC. Various techniques including fluorescence confocal microscopy, flow cytometry and Western blots were utilized to investigate the non-canonical role STAT3 plays during adipogenesis. Mitochondrial isolations were performed to investigate the levels of STAT3 in the mitochondria during differentiation. Further to this, an impedance based real time differentiation assay was developed using the xCELLigence Real Time Cell Analyser to monitor differentiation and the affects various compounds, including a STAT3 inhibitor, have on differentiation. Results indicate that upon induction of differentiation, levels of mitochondrial pSTAT3S727 dramatically decrease and leave the mitochondria. This corresponds to increasing levels of ROS. The canonical active form of STAT3 following phosphorylation on tyrosine 705 (pSTAT3Y705) was found to decrease and lose its nuclear localization. These initial results indicate that STAT3 plays an important non-canonical role in the mitochondria during differentiation.
- Full Text:
- Date Issued: 2014
- Authors: Kramer, Adam Hildyard
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/54632 , vital:26595
- Description: Stem cells have the ability to differentiate into a myriad of different cell types. The understanding of the differentiation process is of paramount importance if we are to use these cells in the lab as well as in therapeutics. Here, the levels and localization of the signal transducer and activator of transcription 3 (STAT3), with particular attention focused on the mitochondrial serine 727 phosphorylated form of STAT3 (pSTAT3S727) during differentiation, was investigated. Using the murine preadipocyte progenitor cell line 3T3-L1, as well as adipose derived human mesenchymal stem cells (HMSC-ad) as differentiation models, the relative levels of Reactive Oxygen Species (ROS) and the levels and localization of STAT3 were investigated during the differentiation process. ROS is known to play an important signalling role during differentiation and is well reported during the events of adipogenesis. ROS are generated as a by-product in the Electron Transport Chain (ETC), and it has recently been reported that pSTAT3S727 plays an important role at complex I of the ETC. Various techniques including fluorescence confocal microscopy, flow cytometry and Western blots were utilized to investigate the non-canonical role STAT3 plays during adipogenesis. Mitochondrial isolations were performed to investigate the levels of STAT3 in the mitochondria during differentiation. Further to this, an impedance based real time differentiation assay was developed using the xCELLigence Real Time Cell Analyser to monitor differentiation and the affects various compounds, including a STAT3 inhibitor, have on differentiation. Results indicate that upon induction of differentiation, levels of mitochondrial pSTAT3S727 dramatically decrease and leave the mitochondria. This corresponds to increasing levels of ROS. The canonical active form of STAT3 following phosphorylation on tyrosine 705 (pSTAT3Y705) was found to decrease and lose its nuclear localization. These initial results indicate that STAT3 plays an important non-canonical role in the mitochondria during differentiation.
- Full Text:
- Date Issued: 2014
Genetic algorithm for Artificial Neural Network training for the purpose of Automated Part Recognition
- Authors: Buys, Stefan
- Date: 2012
- Subjects: Genetic algorithms , Software architecture
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9648 , http://hdl.handle.net/10948/d1008356 , Genetic algorithms , Software architecture
- Description: Object or part recognition is of major interest in industrial environments. Current methods implement expensive camera based solutions. There is a need for a cost effective alternative to be developed. One of the proposed methods is to overcome the hardware, camera, problem by implementing a software solution. Artificial Neural Networks (ANN) are to be used as the underlying intelligent software as they have high tolerance for noise and have the ability to generalize. A colleague has implemented a basic ANN based system comprising of an ANN and three cost effective laser distance sensors. However, the system is only able to identify 3 different parts and needed hard coding changes made by trial and error. This is not practical for industrial use in a production environment where there are a large quantity of different parts to be identified that change relatively regularly. The ability to easily train more parts is required. Difficulties associated with traditional mathematically guided training methods are discussed, which leads to the development of a Genetic Algorithm (GA) based evolutionary training method that overcomes these difficulties and makes accurate part recognition possible. An ANN hybridised with GA training is introduced and a general solution encoding scheme which is used to encode the required ANN connection weights. Experimental tests were performed in order to determine the ideal GA performance and control parameters as studies have indicated that different GA control parameters can lead to large differences in training accuracy. After performing these tests, the training accuracy was analyzed by investigation into GA performance as well as hardware based part recognition performance. This analysis identified the ideal GA control parameters when training an ANN for the purpose of part recognition and showed that the ANN generally trained well and could generalize well on data not presented to it during training.
- Full Text:
- Date Issued: 2012
- Authors: Buys, Stefan
- Date: 2012
- Subjects: Genetic algorithms , Software architecture
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9648 , http://hdl.handle.net/10948/d1008356 , Genetic algorithms , Software architecture
- Description: Object or part recognition is of major interest in industrial environments. Current methods implement expensive camera based solutions. There is a need for a cost effective alternative to be developed. One of the proposed methods is to overcome the hardware, camera, problem by implementing a software solution. Artificial Neural Networks (ANN) are to be used as the underlying intelligent software as they have high tolerance for noise and have the ability to generalize. A colleague has implemented a basic ANN based system comprising of an ANN and three cost effective laser distance sensors. However, the system is only able to identify 3 different parts and needed hard coding changes made by trial and error. This is not practical for industrial use in a production environment where there are a large quantity of different parts to be identified that change relatively regularly. The ability to easily train more parts is required. Difficulties associated with traditional mathematically guided training methods are discussed, which leads to the development of a Genetic Algorithm (GA) based evolutionary training method that overcomes these difficulties and makes accurate part recognition possible. An ANN hybridised with GA training is introduced and a general solution encoding scheme which is used to encode the required ANN connection weights. Experimental tests were performed in order to determine the ideal GA performance and control parameters as studies have indicated that different GA control parameters can lead to large differences in training accuracy. After performing these tests, the training accuracy was analyzed by investigation into GA performance as well as hardware based part recognition performance. This analysis identified the ideal GA control parameters when training an ANN for the purpose of part recognition and showed that the ANN generally trained well and could generalize well on data not presented to it during training.
- Full Text:
- Date Issued: 2012
The genetic integrity of Labeo capensis and L. umbratus (Cyprinidae) in South Africa in relation to inter-basin water transfer schemes
- Authors: Ramoejane, Mpho
- Date: 2011
- Subjects: Cyprinidae -- South Africa -- Darlington Dam , Cyprinidae -- Namibia -- Hardap Dam , Labeo -- South Africa -- Darlington Dam , Labeo -- Namibia -- Hardap Dam , Fishes -- Hybridisation -- South Africa -- Darlington Dam , Fishes -- Hybridisation -- Namibia -- Hardap Dam , Fishes -- Anatomy -- South Africa -- Darlington Dam , Fishes -- Anatomy -- Namibia -- Hardap Dam , Water transfer -- South Africa-- Darlington Dam , Water transfer -- Namibia -- Hardap Dam
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5367 , http://hdl.handle.net/10962/d1013049
- Description: The Orange-Fish and Cookhouse tunnels that are part of a major inter-basin water transfer scheme (IBT) act as a pathway for several fish species from the Orange River system to enter the Great Fish and Sundays River systems in South Africa. These include Labeo capensis and L. umbratus. Labeo capensis was restricted to the Orange River system before the inter-basin water transfer scheme. Labeo umbratus occurred naturally in the Orange River and in southern flowing river systems. Previous studies showed that the two species hybridise in Hardap Dam, located in a tributary of the Orange River system in Namibia. There are also unconfirmed reports of hybrids from Darlington Dam on the Sundays River system. The aim of the thesis was to confirm hybridisation in Hardap Dam, assess whether hybridisation between L. capensis and L. umbratus has occurred in Darlington Dam and to gain a better understanding of the diversity of these two species. Morphology (morphometrics and meristics), a nuclear S7 intron and the mitochondrial cytochrome ♭ gene were used to assess for hybridisation. A total of 275 specimens were analysed from across the geographical range of the two species. The two species could be distinguished using morphometrics (dorsal fin base, interorbital width and operculum to eye distance) and meristics (lateral line, origin of the dorsal fin to lateral line, origin of the pelvic fin to lateral line and caudal peduncle scale counts) characters. Hybrids from Hardap and Darlington dams were placed between the two species clusters. Labeo umbratus from the Orange River and southern flowing rivers formed a single cluster. The two species could also be distinguished from each other with six nuclear DNA mutations and hybrids were heterozygous at such sites in both dams. Labeo umbratus populations from the Orange River and southern flowing rivers (Gouritz, Gamtoos, Sundays, Bushmans, Great Fish and Nahoon) formed a single lineage. Analysis of mitochondrial DNA, however, revealed that L. umbratus populations from the Orange River and southern flowing rivers were two lineages that differ from each other by 5 mutations. Labeo capensis could be differentiated from both these lineages. Being maternally inherited, mitochondrial DNA did not reveal hybridisation, but ten specimens with L. capensis haplotypes were found in the Darlington Dam. In Hardap Dam, however, it appears that only L. capensis mitochondrial DNA haplotypes persist, despite morphological and nuclear DNA analysis suggesting that both morphs and hybrids of the two species occur. The genetic integrity of these Labeo species has therefore been compromised in at least Hardap and Darlington dams. The Great Fish and Sundays populations are considered to be under threat of complete introgression. The Kat River and Slagboom Dam populations that were isolated before the IBTs have to remain isolated to protect the genetic integrity of the southern lineage of L. umbratus in these two systems.
- Full Text:
- Date Issued: 2011
- Authors: Ramoejane, Mpho
- Date: 2011
- Subjects: Cyprinidae -- South Africa -- Darlington Dam , Cyprinidae -- Namibia -- Hardap Dam , Labeo -- South Africa -- Darlington Dam , Labeo -- Namibia -- Hardap Dam , Fishes -- Hybridisation -- South Africa -- Darlington Dam , Fishes -- Hybridisation -- Namibia -- Hardap Dam , Fishes -- Anatomy -- South Africa -- Darlington Dam , Fishes -- Anatomy -- Namibia -- Hardap Dam , Water transfer -- South Africa-- Darlington Dam , Water transfer -- Namibia -- Hardap Dam
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5367 , http://hdl.handle.net/10962/d1013049
- Description: The Orange-Fish and Cookhouse tunnels that are part of a major inter-basin water transfer scheme (IBT) act as a pathway for several fish species from the Orange River system to enter the Great Fish and Sundays River systems in South Africa. These include Labeo capensis and L. umbratus. Labeo capensis was restricted to the Orange River system before the inter-basin water transfer scheme. Labeo umbratus occurred naturally in the Orange River and in southern flowing river systems. Previous studies showed that the two species hybridise in Hardap Dam, located in a tributary of the Orange River system in Namibia. There are also unconfirmed reports of hybrids from Darlington Dam on the Sundays River system. The aim of the thesis was to confirm hybridisation in Hardap Dam, assess whether hybridisation between L. capensis and L. umbratus has occurred in Darlington Dam and to gain a better understanding of the diversity of these two species. Morphology (morphometrics and meristics), a nuclear S7 intron and the mitochondrial cytochrome ♭ gene were used to assess for hybridisation. A total of 275 specimens were analysed from across the geographical range of the two species. The two species could be distinguished using morphometrics (dorsal fin base, interorbital width and operculum to eye distance) and meristics (lateral line, origin of the dorsal fin to lateral line, origin of the pelvic fin to lateral line and caudal peduncle scale counts) characters. Hybrids from Hardap and Darlington dams were placed between the two species clusters. Labeo umbratus from the Orange River and southern flowing rivers formed a single cluster. The two species could also be distinguished from each other with six nuclear DNA mutations and hybrids were heterozygous at such sites in both dams. Labeo umbratus populations from the Orange River and southern flowing rivers (Gouritz, Gamtoos, Sundays, Bushmans, Great Fish and Nahoon) formed a single lineage. Analysis of mitochondrial DNA, however, revealed that L. umbratus populations from the Orange River and southern flowing rivers were two lineages that differ from each other by 5 mutations. Labeo capensis could be differentiated from both these lineages. Being maternally inherited, mitochondrial DNA did not reveal hybridisation, but ten specimens with L. capensis haplotypes were found in the Darlington Dam. In Hardap Dam, however, it appears that only L. capensis mitochondrial DNA haplotypes persist, despite morphological and nuclear DNA analysis suggesting that both morphs and hybrids of the two species occur. The genetic integrity of these Labeo species has therefore been compromised in at least Hardap and Darlington dams. The Great Fish and Sundays populations are considered to be under threat of complete introgression. The Kat River and Slagboom Dam populations that were isolated before the IBTs have to remain isolated to protect the genetic integrity of the southern lineage of L. umbratus in these two systems.
- Full Text:
- Date Issued: 2011
Predation by alien largemouth bass, Micropterus salmoides Lacepéde 1802 (Centrarchidae: Perciformes), on indigenous marine fish species in the Kowie System, South Africa
- Authors: Magoro, Mandla Leon
- Date: 2014
- Subjects: Largemouth bass -- South Africa -- Port Alfred , Largemouth bass -- Food -- South Africa -- Port Alfred , Estuarine fishes -- South Africa -- Port Alfred , Estuarine fishes -- Predators of , Centrarchidae , Fishes -- Effect of human beings on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5851 , http://hdl.handle.net/10962/d1011939 , Largemouth bass -- South Africa -- Port Alfred , Largemouth bass -- Food -- South Africa -- Port Alfred , Estuarine fishes -- South Africa -- Port Alfred , Estuarine fishes -- Predators of , Centrarchidae , Fishes -- Effect of human beings on
- Description: Estuaries serve as nursery areas for a large number of estuary-associated fish species. Some of these taxa also use river catchments as nursery areas. During the upstream migration of this latter group, the juveniles are prone to predation by native and alien predatory fish inhabiting the system. The rate of invasion of ecosystems by alien organisms can be directly linked to anthropogenic influences, including both intentional and unintentional introductions by alien organisms into new regions. The largemouth bass, Micropterus salmoides, is a facultative piscivorous fish that has been successfully introduced worldwide for the main purpose of sport fishing. Where introduced, it has been found to negatively impact native fish and invertebrate species through predation, competitive exclusion and displacement of indigenous fish species. The aim of this thesis was to investigate the predatory impact of largemouth bass on the estuary-associated Cape moony Monodactylus falciformis, Cape stumpnose Rhabdosargus holubi and freshwater mullet Myxus capensis in the lower Kowie River of the Eastern Cape Province, South Africa. A combination of approaches was employed during this study. Stomach contents, stable isotopes and fatty acid analyses were employed for the reconstruction of the diet of largemouth bass. Acoustic telemetry was used to elucidate largemouth bass movements, particularly their ability to enter the upper reaches of the Kowie Estuary. Stomach contents and stable isotopes analyses showed that M. salmoides exhibit an ontogenic shift in diet, with small and medium sized individuals consuming the higher proportion of fish prey, while large sized individuals mostly consumed invertebrates such as crabs (Potamonautes sidneyi) and Odonata larvae, while consuming only a small proportion of estuary-associated fish. Fatty acid analysis only showed a direct connection between the fatty acid profiles of largemouth bass and those of M. capensis and M. falciformis. The acoustic telemetry results indicated that some M. salmoides individuals periodically move into the upper reaches of the estuary following river flood events. The results obtained from all these approaches highlight the risk posed by largemouth bass introductions on indigenous fish species, particularly those that enter the areas occupied by these top predators.
- Full Text:
- Date Issued: 2014
- Authors: Magoro, Mandla Leon
- Date: 2014
- Subjects: Largemouth bass -- South Africa -- Port Alfred , Largemouth bass -- Food -- South Africa -- Port Alfred , Estuarine fishes -- South Africa -- Port Alfred , Estuarine fishes -- Predators of , Centrarchidae , Fishes -- Effect of human beings on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5851 , http://hdl.handle.net/10962/d1011939 , Largemouth bass -- South Africa -- Port Alfred , Largemouth bass -- Food -- South Africa -- Port Alfred , Estuarine fishes -- South Africa -- Port Alfred , Estuarine fishes -- Predators of , Centrarchidae , Fishes -- Effect of human beings on
- Description: Estuaries serve as nursery areas for a large number of estuary-associated fish species. Some of these taxa also use river catchments as nursery areas. During the upstream migration of this latter group, the juveniles are prone to predation by native and alien predatory fish inhabiting the system. The rate of invasion of ecosystems by alien organisms can be directly linked to anthropogenic influences, including both intentional and unintentional introductions by alien organisms into new regions. The largemouth bass, Micropterus salmoides, is a facultative piscivorous fish that has been successfully introduced worldwide for the main purpose of sport fishing. Where introduced, it has been found to negatively impact native fish and invertebrate species through predation, competitive exclusion and displacement of indigenous fish species. The aim of this thesis was to investigate the predatory impact of largemouth bass on the estuary-associated Cape moony Monodactylus falciformis, Cape stumpnose Rhabdosargus holubi and freshwater mullet Myxus capensis in the lower Kowie River of the Eastern Cape Province, South Africa. A combination of approaches was employed during this study. Stomach contents, stable isotopes and fatty acid analyses were employed for the reconstruction of the diet of largemouth bass. Acoustic telemetry was used to elucidate largemouth bass movements, particularly their ability to enter the upper reaches of the Kowie Estuary. Stomach contents and stable isotopes analyses showed that M. salmoides exhibit an ontogenic shift in diet, with small and medium sized individuals consuming the higher proportion of fish prey, while large sized individuals mostly consumed invertebrates such as crabs (Potamonautes sidneyi) and Odonata larvae, while consuming only a small proportion of estuary-associated fish. Fatty acid analysis only showed a direct connection between the fatty acid profiles of largemouth bass and those of M. capensis and M. falciformis. The acoustic telemetry results indicated that some M. salmoides individuals periodically move into the upper reaches of the estuary following river flood events. The results obtained from all these approaches highlight the risk posed by largemouth bass introductions on indigenous fish species, particularly those that enter the areas occupied by these top predators.
- Full Text:
- Date Issued: 2014
The socio-economic importance of indigenous vegetables to the Ntuze smallholder farming community in KwaZulu-Natal, South Africa
- Authors: Qwabe, Qinisani Nhlakanipho
- Date: 2019
- Subjects: Indigenous crops -- Economic aspects -- South Africa -- KwaZulu Natal , Vegetables -- South Africa -- KwaZulu Natal Farmers -- Economic aspects -- South Africa -- KwaZulu Natal , Farms, Small -- South Africa -- KwaZulu Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/30287 , vital:30927
- Description: South Africa continues to face multiple socio-economic challenges – one of the direst being food insecurity, especially in rural areas. Compounding the challenges is the impact of changing weather patterns on agriculture. The current study presents evidence indicating that indigenous vegetables provide a sustainable yet inexpensive answer to several of these challenges. The research was aimed at determining the socio-economic impact of indigenous vegetables in the Ntuze community of northern KwaZulu-Natal. A mixed-methods methodology was employed to achieve a holistic understanding of the relationship between the use of indigenous vegetables and socio-economic influences. The study discovered that indigenous vegetables play an important role in the livelihood strategy of this rural farming community. Findings revealed that the utilisation of indigenous vegetables makes an essential contribution to the livelihoods and well-being of the Ntuze people, especially in terms of curbing food poverty, income generation and medicinal benefits. However, the value of these vegetables was found to be appreciated mostly by the elderly. Conclusions therefore indicate a possible decline in future production and use of indigenous vegetables if indigenous knowledge is not passed on to the next generation. Integration of indigenous knowledge in agricultural technology transfer programmes is therefore vital to promote production of indigenous vegetables as a sustainable food resource. Actively addressing the stigma attached to indigenous vegetables as being “low-status food” is also crucial to enhance perception and understanding of the value of these vegetables. This would contribute to both preserving cultural heritage and conserving valuable indigenous flora. Such intervention would safeguard this sustainable and renewable resource in its pivotal socio-economic role in terms of rural food security as is substantiated by this study.
- Full Text:
- Date Issued: 2019
- Authors: Qwabe, Qinisani Nhlakanipho
- Date: 2019
- Subjects: Indigenous crops -- Economic aspects -- South Africa -- KwaZulu Natal , Vegetables -- South Africa -- KwaZulu Natal Farmers -- Economic aspects -- South Africa -- KwaZulu Natal , Farms, Small -- South Africa -- KwaZulu Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/30287 , vital:30927
- Description: South Africa continues to face multiple socio-economic challenges – one of the direst being food insecurity, especially in rural areas. Compounding the challenges is the impact of changing weather patterns on agriculture. The current study presents evidence indicating that indigenous vegetables provide a sustainable yet inexpensive answer to several of these challenges. The research was aimed at determining the socio-economic impact of indigenous vegetables in the Ntuze community of northern KwaZulu-Natal. A mixed-methods methodology was employed to achieve a holistic understanding of the relationship between the use of indigenous vegetables and socio-economic influences. The study discovered that indigenous vegetables play an important role in the livelihood strategy of this rural farming community. Findings revealed that the utilisation of indigenous vegetables makes an essential contribution to the livelihoods and well-being of the Ntuze people, especially in terms of curbing food poverty, income generation and medicinal benefits. However, the value of these vegetables was found to be appreciated mostly by the elderly. Conclusions therefore indicate a possible decline in future production and use of indigenous vegetables if indigenous knowledge is not passed on to the next generation. Integration of indigenous knowledge in agricultural technology transfer programmes is therefore vital to promote production of indigenous vegetables as a sustainable food resource. Actively addressing the stigma attached to indigenous vegetables as being “low-status food” is also crucial to enhance perception and understanding of the value of these vegetables. This would contribute to both preserving cultural heritage and conserving valuable indigenous flora. Such intervention would safeguard this sustainable and renewable resource in its pivotal socio-economic role in terms of rural food security as is substantiated by this study.
- Full Text:
- Date Issued: 2019
Problems experienced by project managers on housing projects
- Mtshekexe, Ntombesibini Octavia
- Authors: Mtshekexe, Ntombesibini Octavia
- Date: 2014
- Subjects: Project management , Housing rehabilitation , Public housing -- South Africa , Building -- Superintendence
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9675 , http://hdl.handle.net/10948/d1020930
- Description: Housing projects are amongst the South African (SA) Government’s major focus points with reference to budgeting for its financial years. The delivery of these housing projects is taking place at a slower rate than anticipated due to the problems experienced in the processes involved in their management. The project management shortcomings are a major setback with reference to the delivery of houses to the communities. It is, however, stated by the Constitution of the Republic of South Africa (RSA) that every South African citizen has a right to housing and that the Local Government, as the closest entity to communities, must do whatever it possibly can to ensure timeous service delivery to its communities. The objective of this research is to ascertain the nature and extent of project management problems experienced by municipal project managers, the impact thereof and potential solutions to the identified problems. The study is intended to advise on possible ways to address project management problems arising during the implementation of housing projects in the Nelson Mandela Bay Municipality (NMBM) through investigation and analysis of information gathered in both theory and practice. Project management is a process of controlling the achievement of project objectives through the application of knowledge, skills, tools and techniques. For a housing project the main objective is to deliver safe, quality houses on time and within budget. Problems which hinder the delivery of housing projects relate to cost, time and the quality of housing projects delivered. The fact that these key project objectives are not met needs to be addressed urgently in order to improve the housing delivery. The literature reviewed and the feedback from the surveys; indicate that these problems have a vast impact on housing projects. The survey also reveals that it is not only housing Project Managers (PMs) that are unable to perform well, but also other parties involved in the project management of housing projects and which play a great role in project management failures of housing projects. Some of these parties are consultants and contractors. The lack of performance by project managers affects the delivery of houses negatively in the NMBM.
- Full Text:
- Date Issued: 2014
- Authors: Mtshekexe, Ntombesibini Octavia
- Date: 2014
- Subjects: Project management , Housing rehabilitation , Public housing -- South Africa , Building -- Superintendence
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9675 , http://hdl.handle.net/10948/d1020930
- Description: Housing projects are amongst the South African (SA) Government’s major focus points with reference to budgeting for its financial years. The delivery of these housing projects is taking place at a slower rate than anticipated due to the problems experienced in the processes involved in their management. The project management shortcomings are a major setback with reference to the delivery of houses to the communities. It is, however, stated by the Constitution of the Republic of South Africa (RSA) that every South African citizen has a right to housing and that the Local Government, as the closest entity to communities, must do whatever it possibly can to ensure timeous service delivery to its communities. The objective of this research is to ascertain the nature and extent of project management problems experienced by municipal project managers, the impact thereof and potential solutions to the identified problems. The study is intended to advise on possible ways to address project management problems arising during the implementation of housing projects in the Nelson Mandela Bay Municipality (NMBM) through investigation and analysis of information gathered in both theory and practice. Project management is a process of controlling the achievement of project objectives through the application of knowledge, skills, tools and techniques. For a housing project the main objective is to deliver safe, quality houses on time and within budget. Problems which hinder the delivery of housing projects relate to cost, time and the quality of housing projects delivered. The fact that these key project objectives are not met needs to be addressed urgently in order to improve the housing delivery. The literature reviewed and the feedback from the surveys; indicate that these problems have a vast impact on housing projects. The survey also reveals that it is not only housing Project Managers (PMs) that are unable to perform well, but also other parties involved in the project management of housing projects and which play a great role in project management failures of housing projects. Some of these parties are consultants and contractors. The lack of performance by project managers affects the delivery of houses negatively in the NMBM.
- Full Text:
- Date Issued: 2014
The thermal physiology of Stenopelmus rufinasus and Neohydronomus affinis (Coleoptera: Curculionidae), biological control agents for the invasive alien aquatic weeds Azolla filiculoides and Pistia stratiotes respectively
- Authors: Mvandaba, Sisanda F
- Date: 2018
- Subjects: Beetles -- South Africa , Curculionidae -- South Africa , Azolla filiculoides -- South Africa , Water lettuce -- South Africa , Aquatic weeds -- Biological control -- South Africa , Stenopelmus rufinasus , Neohydronomus affinis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62362 , vital:28158
- Description: Water lettuce, Pistia stratiotes L. (Araceae), and red water fern, Azolla filiculoides Lam. (Azollaceae), are floating aquatic macrophytes that have become problematic invaders in numerous South African waterbodies. Two weevils, Neohydronomus affinis Hustache 1926 (Coleoptera: Curculionidae) and Stenopelmus rufinasus Gyllenhal 1936 (Coleoptera: Curculionidae), are successful biological control agents of these two species, respectively, in South Africa. However, nothing is known about the thermal physiology of these two species Therefore, the aim of this study was to investigate the thermal physiologies of these two species to explain their establishment, distribution and impact in the field. Laboratory based thermal physiology trials showed that both weevils were widely tolerant of cold and warm temperatures. The CTmin of N. affinis was determined to be 5.5 ± 0.312°C and the CTmax was 44 ± 0.697°C, while the CTmin of S. rufinasus was 5.4 ± 0.333°C and the CTmax was 44.5 ± 0.168°C. In addition, the lower lethal temperatures were -9.8 ± 0.053°C and -7.2 ± 0.19°C, and the upper lethal temperatures were 42.8 ± 0.053°C and 41.9 ± 0.19°C respectively. These results suggest that both species should not be limited by cold winter temperatures, as previously thought. This is evident in the field, where S. rufinasus has established widely on A. filiculoides, despite local cold climates in some areas of the plant’s distribution. Even though N. affinis has a similar thermal range, and should therefore theoretically reflect a similar distribution to S. rufinasus throughout South Africa, its distribution is limited by the range of its host, which is restricted to the warmer regions of the country, as is its biocontrol agent. Using the reduced major axis regression method, the development for N. affinis was described using the formulay=12.976x+435.24, while the development of S. rufinasus was described by y=13.6x+222.45. These results showed that S. rufinasus develops much faster, in fact almost twice as quickly, than N. affinis. Using these formulae and temperature data obtained from the South African Weather Service, N. affinis was predicted to complete between 4 and 9 generations per year in South Africa, while S. rufinasus was predicted to complete between 5 and 14 generations per year around the country. This study showed that although the native range of these two species is warm temperate to tropical, they possess sufficient thermal plasticity to not only establish, but also damage their respective host plants in far cooler climates. Thus, in South Africa N. affinis and S. rufinasus are limited by the distribution of their target weeds and not climate.
- Full Text:
- Date Issued: 2018
- Authors: Mvandaba, Sisanda F
- Date: 2018
- Subjects: Beetles -- South Africa , Curculionidae -- South Africa , Azolla filiculoides -- South Africa , Water lettuce -- South Africa , Aquatic weeds -- Biological control -- South Africa , Stenopelmus rufinasus , Neohydronomus affinis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62362 , vital:28158
- Description: Water lettuce, Pistia stratiotes L. (Araceae), and red water fern, Azolla filiculoides Lam. (Azollaceae), are floating aquatic macrophytes that have become problematic invaders in numerous South African waterbodies. Two weevils, Neohydronomus affinis Hustache 1926 (Coleoptera: Curculionidae) and Stenopelmus rufinasus Gyllenhal 1936 (Coleoptera: Curculionidae), are successful biological control agents of these two species, respectively, in South Africa. However, nothing is known about the thermal physiology of these two species Therefore, the aim of this study was to investigate the thermal physiologies of these two species to explain their establishment, distribution and impact in the field. Laboratory based thermal physiology trials showed that both weevils were widely tolerant of cold and warm temperatures. The CTmin of N. affinis was determined to be 5.5 ± 0.312°C and the CTmax was 44 ± 0.697°C, while the CTmin of S. rufinasus was 5.4 ± 0.333°C and the CTmax was 44.5 ± 0.168°C. In addition, the lower lethal temperatures were -9.8 ± 0.053°C and -7.2 ± 0.19°C, and the upper lethal temperatures were 42.8 ± 0.053°C and 41.9 ± 0.19°C respectively. These results suggest that both species should not be limited by cold winter temperatures, as previously thought. This is evident in the field, where S. rufinasus has established widely on A. filiculoides, despite local cold climates in some areas of the plant’s distribution. Even though N. affinis has a similar thermal range, and should therefore theoretically reflect a similar distribution to S. rufinasus throughout South Africa, its distribution is limited by the range of its host, which is restricted to the warmer regions of the country, as is its biocontrol agent. Using the reduced major axis regression method, the development for N. affinis was described using the formulay=12.976x+435.24, while the development of S. rufinasus was described by y=13.6x+222.45. These results showed that S. rufinasus develops much faster, in fact almost twice as quickly, than N. affinis. Using these formulae and temperature data obtained from the South African Weather Service, N. affinis was predicted to complete between 4 and 9 generations per year in South Africa, while S. rufinasus was predicted to complete between 5 and 14 generations per year around the country. This study showed that although the native range of these two species is warm temperate to tropical, they possess sufficient thermal plasticity to not only establish, but also damage their respective host plants in far cooler climates. Thus, in South Africa N. affinis and S. rufinasus are limited by the distribution of their target weeds and not climate.
- Full Text:
- Date Issued: 2018
Seasonal changes in the physiological and hormonal aspects of reproduction in the male long-fingered bat, Miniopterus schreibersii (Mammalia : Chiroptera)
- Authors: Paton, Joy Carol
- Date: 1989
- Subjects: Bats -- Reproduction
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5590 , http://hdl.handle.net/10962/d1002040
- Description: The reproductive cycle of Miniopterus schreibersii is modified as a consequence of a period of torpor/hibernation over the winter months. This bat is one of three genera which employ the reproductive strategy of delayed implantation in which the blastocyst remains free in the uterine lumen during the winter months. Spermatogenesis is initiated in February, with spermiogenesis occuring in March. Copulation, ovulation and fertilization takes place between March and May after which the testes regress and remain in an inactive condition until the following summer. Activity in the accessory gland complex is initiated in March and continues until early June. Plasma testosterone concentrations reach a peak in May, decline over the winter months and reach a second, unexplainable peak in October. Leydig cells are secretorily active in February/early March after which they undergo vacuolation and final degeneration. The question of the life cyle of Leydig cells is addressed and an increase in a certain cell after Leydig cell activity suggests that this cell type may be the precursor of Leydig cells.
- Full Text:
- Date Issued: 1989
- Authors: Paton, Joy Carol
- Date: 1989
- Subjects: Bats -- Reproduction
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5590 , http://hdl.handle.net/10962/d1002040
- Description: The reproductive cycle of Miniopterus schreibersii is modified as a consequence of a period of torpor/hibernation over the winter months. This bat is one of three genera which employ the reproductive strategy of delayed implantation in which the blastocyst remains free in the uterine lumen during the winter months. Spermatogenesis is initiated in February, with spermiogenesis occuring in March. Copulation, ovulation and fertilization takes place between March and May after which the testes regress and remain in an inactive condition until the following summer. Activity in the accessory gland complex is initiated in March and continues until early June. Plasma testosterone concentrations reach a peak in May, decline over the winter months and reach a second, unexplainable peak in October. Leydig cells are secretorily active in February/early March after which they undergo vacuolation and final degeneration. The question of the life cyle of Leydig cells is addressed and an increase in a certain cell after Leydig cell activity suggests that this cell type may be the precursor of Leydig cells.
- Full Text:
- Date Issued: 1989