Press cutting: 'King Kong' is most exciting show seen in years
- "R", Daily News
- Authors: "R" , Daily News
- Date: 1959-05-12
- Subjects: King Kong (Musical) -- History and critisism , Jazz -- South Africa , Musicals -- South Africa -- History and critisism , Music -- South Africa , Matshikiza, Todd, 1921-1968 -- King Kong
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/42004 , vital:25155 , MS 20 066 , This image is held at the Cory Library for Humanities Research at Rhodes University. For further information contact cory@ru.ac.za. The digitisation of this image was made possible through a generous grant received from the Andrew W. Mellon Foundation 2014-2017.
- Full Text:
- Date Issued: 1959-05-12
- Authors: "R" , Daily News
- Date: 1959-05-12
- Subjects: King Kong (Musical) -- History and critisism , Jazz -- South Africa , Musicals -- South Africa -- History and critisism , Music -- South Africa , Matshikiza, Todd, 1921-1968 -- King Kong
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/42004 , vital:25155 , MS 20 066 , This image is held at the Cory Library for Humanities Research at Rhodes University. For further information contact cory@ru.ac.za. The digitisation of this image was made possible through a generous grant received from the Andrew W. Mellon Foundation 2014-2017.
- Full Text:
- Date Issued: 1959-05-12
Programme of the centenary celebrations in commemoration of the arrival of the 1820 British settlers of Albany, to be held at Port Elizabeth on April 9th and at Grahamstown on April 11th 1921
- 1820 Settlers' Association of South Africa
- Authors: 1820 Settlers' Association of South Africa
- Date: 1921
- Subjects: British settlers of 1820 (South Africa) -- Celebration -- 1921 , South Africa -- History -- Frontier Wars, 1811-1878 , Eastern Cape (South Africa) -- History -- Pictorial works
- Language: English
- Type: programme , text
- Identifier: http://hdl.handle.net/10962/113427 , vital:33773 , Cory Library for Humanities Research
- Description: Programme of the centenary celebrations in commemoration of the arrival of the 1820 British settlers of Albany, to be held at Port Elizabeth on April 9th and at Grahamstown on April 11th 1921 : containing a descriptive guide to the motor route through the Eastern Province to be travelled by Major-General H R H Price Arthur of Connaught ..., Governor-General of the Union of South Africa, accompanied by H R H Princess Arthur of Connaught, at the close of the celebrations.
- Full Text:
- Date Issued: 1921
- Authors: 1820 Settlers' Association of South Africa
- Date: 1921
- Subjects: British settlers of 1820 (South Africa) -- Celebration -- 1921 , South Africa -- History -- Frontier Wars, 1811-1878 , Eastern Cape (South Africa) -- History -- Pictorial works
- Language: English
- Type: programme , text
- Identifier: http://hdl.handle.net/10962/113427 , vital:33773 , Cory Library for Humanities Research
- Description: Programme of the centenary celebrations in commemoration of the arrival of the 1820 British settlers of Albany, to be held at Port Elizabeth on April 9th and at Grahamstown on April 11th 1921 : containing a descriptive guide to the motor route through the Eastern Province to be travelled by Major-General H R H Price Arthur of Connaught ..., Governor-General of the Union of South Africa, accompanied by H R H Princess Arthur of Connaught, at the close of the celebrations.
- Full Text:
- Date Issued: 1921
But is it publishable? Mastering the MMed message
- Authors: A G Parrish , E S Grossman,
- Date: 202
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/3353 , vital:43318 , http://www.samj.org.za/index.php/samj/article/view/13033
- Description: The research requirement for South African specialist registration offers opportunities and challenges. For some clinicians it may spark a lifelong interest in clinical investigation, while for many others it may provide a potential publication opportunity. Integrating the specific requirements of an MMed mini-dissertation with those of standard medical publications can be difficult for first-time authors and their supervisors; published guidance caters to full-length laboratory Master’s or doctoral research. We suggest that research is more likely to be publishable if it is locally relevant, has a clear clinical message and is coherently presented.
- Full Text:
- Date Issued: 202
- Authors: A G Parrish , E S Grossman,
- Date: 202
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/3353 , vital:43318 , http://www.samj.org.za/index.php/samj/article/view/13033
- Description: The research requirement for South African specialist registration offers opportunities and challenges. For some clinicians it may spark a lifelong interest in clinical investigation, while for many others it may provide a potential publication opportunity. Integrating the specific requirements of an MMed mini-dissertation with those of standard medical publications can be difficult for first-time authors and their supervisors; published guidance caters to full-length laboratory Master’s or doctoral research. We suggest that research is more likely to be publishable if it is locally relevant, has a clear clinical message and is coherently presented.
- Full Text:
- Date Issued: 202
Essential medicine selection during the COVID-19 pandemic: Enabling access in uncharted territory
- Authors: A G Parrish
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/3364 , vital:43319 , http://www.samj.org.za/index.php/samj/article/view/13086
- Description: The COVID-19 pandemic requires urgent decisions regarding treatment policy in the face of rapidly evolving evidence. In response, the South African Essential Medicines List Committee established a subcommittee to systematically review and appraise emerging evidence, within very short timelines, in order to inform the National Department of Health COVID-19 treatment guidelines. To date, the subcommittee has reviewed 14 potential treatments, and made recommendations based on local context, feasibility, resource requirements and equity. Here we describe the rapid review and evidence-to-decision process, using remdesivir and dexamethasone as examples. Our experience is that conducting rapid reviews is a practical and efficient way to address medicine policy questions under pandemic conditions.
- Full Text:
- Date Issued: 2020
- Authors: A G Parrish
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/3364 , vital:43319 , http://www.samj.org.za/index.php/samj/article/view/13086
- Description: The COVID-19 pandemic requires urgent decisions regarding treatment policy in the face of rapidly evolving evidence. In response, the South African Essential Medicines List Committee established a subcommittee to systematically review and appraise emerging evidence, within very short timelines, in order to inform the National Department of Health COVID-19 treatment guidelines. To date, the subcommittee has reviewed 14 potential treatments, and made recommendations based on local context, feasibility, resource requirements and equity. Here we describe the rapid review and evidence-to-decision process, using remdesivir and dexamethasone as examples. Our experience is that conducting rapid reviews is a practical and efficient way to address medicine policy questions under pandemic conditions.
- Full Text:
- Date Issued: 2020
The endocannabinoid system in inflammatory bowel system
- Authors: Ababio, Frank James Kweku
- Date: 2014
- Subjects: Inflammatory bowel diseases , Gastrointestinal system , Gastrointestinal system -- Diseases
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10346 , http://hdl.handle.net/10948/d1020338
- Description: Crohn’s disease (CD) and ulcerative colitis (UC) constitute the two major forms of inflammatory bowel disease (IBD), which are disorders of chronic inflammation in the gastrointestinal tract that are associated with significant morbidity and socioeconomic burden. IBD patients with long-standing intestinal inflammation are more prone to developing colorectal cancer (CRC). Until now, none of the existing IBD treatments is able to heal the mucosal ulcerations satisfactorily. The endocannabinoid system (ECS), which comprises of endogenous cannabinoid ligands, their receptors, and metabolic enzymes, has been implicated in gut homeostasis, visceral sensation, inflammation and gastrointestinal motility. Available studies in rodent models of IBD suggest that enhancing the ECS tone may reduce inflammation and improve mucosal integrity. This evidence indicates that the components of the ECS seem well positioned to exert a protective role in IBD and also to offer a great opportunity for therapeutic exploitation. Despite the role of the ECS in the gut, the presence and function of the components of the ECS is not well characterised in human IBD. The primary aim of the study was to investigate the state of the major components of the ECS in human IBD and to establish whether IBD is associated with any changes of the components of the ECS. Cannabinoid CB1 and CB2 receptors, enzymes for endocannabinoid biosynthesis PLC, “LRAT”, NAPE-PLD and DAGL, and endocannabinoid metabolic enzymes FAAH and MAGL were analysed from colonic tissue samples of CD, UC and control patients by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) to determine the relative mRNA expression of the above genes. The RT-qPCR analysis showed that the mRNA expression of PLC, LRAT, and NAPE-PLD were unchanged in both CD and UC, whiles DAGL mRNA was decreased in UC but was unchanged in CD. The endocannabinoid degradation enzymes, FAAH mRNA expression was also unchanged in CD but decreased in UC, whereas the mRNA expression of MAGL was significantly decreased in both CD and UC. NAPE-PLD/FAAH and DAGL/MAGL ratios, an estimation of the balance of AEA and 2-AG levels, showed that AEA and 2-AG levels could be increased and unchanged, respectively, in IBD. The mRNA expression of CB1 was significantly decreased in CD and UC whilst CB2 mRNA expression was unchanged in both forms of IBD. The study demonstrated that the components of the ECS which were investigated were present in colonic tissues of both IBD patients and healthy individuals, but they appear to be off balance in CD and UC patients. The decreased CB1 receptors in IBD patients could be an important modifier in the disease and could also provide a possible pathoaetiological mechanism linking IBD and CRC. Although these findings look promising, more studies with larger sample size are required to characterise the components of the ECS in human IBD.
- Full Text:
- Date Issued: 2014
- Authors: Ababio, Frank James Kweku
- Date: 2014
- Subjects: Inflammatory bowel diseases , Gastrointestinal system , Gastrointestinal system -- Diseases
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10346 , http://hdl.handle.net/10948/d1020338
- Description: Crohn’s disease (CD) and ulcerative colitis (UC) constitute the two major forms of inflammatory bowel disease (IBD), which are disorders of chronic inflammation in the gastrointestinal tract that are associated with significant morbidity and socioeconomic burden. IBD patients with long-standing intestinal inflammation are more prone to developing colorectal cancer (CRC). Until now, none of the existing IBD treatments is able to heal the mucosal ulcerations satisfactorily. The endocannabinoid system (ECS), which comprises of endogenous cannabinoid ligands, their receptors, and metabolic enzymes, has been implicated in gut homeostasis, visceral sensation, inflammation and gastrointestinal motility. Available studies in rodent models of IBD suggest that enhancing the ECS tone may reduce inflammation and improve mucosal integrity. This evidence indicates that the components of the ECS seem well positioned to exert a protective role in IBD and also to offer a great opportunity for therapeutic exploitation. Despite the role of the ECS in the gut, the presence and function of the components of the ECS is not well characterised in human IBD. The primary aim of the study was to investigate the state of the major components of the ECS in human IBD and to establish whether IBD is associated with any changes of the components of the ECS. Cannabinoid CB1 and CB2 receptors, enzymes for endocannabinoid biosynthesis PLC, “LRAT”, NAPE-PLD and DAGL, and endocannabinoid metabolic enzymes FAAH and MAGL were analysed from colonic tissue samples of CD, UC and control patients by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) to determine the relative mRNA expression of the above genes. The RT-qPCR analysis showed that the mRNA expression of PLC, LRAT, and NAPE-PLD were unchanged in both CD and UC, whiles DAGL mRNA was decreased in UC but was unchanged in CD. The endocannabinoid degradation enzymes, FAAH mRNA expression was also unchanged in CD but decreased in UC, whereas the mRNA expression of MAGL was significantly decreased in both CD and UC. NAPE-PLD/FAAH and DAGL/MAGL ratios, an estimation of the balance of AEA and 2-AG levels, showed that AEA and 2-AG levels could be increased and unchanged, respectively, in IBD. The mRNA expression of CB1 was significantly decreased in CD and UC whilst CB2 mRNA expression was unchanged in both forms of IBD. The study demonstrated that the components of the ECS which were investigated were present in colonic tissues of both IBD patients and healthy individuals, but they appear to be off balance in CD and UC patients. The decreased CB1 receptors in IBD patients could be an important modifier in the disease and could also provide a possible pathoaetiological mechanism linking IBD and CRC. Although these findings look promising, more studies with larger sample size are required to characterise the components of the ECS in human IBD.
- Full Text:
- Date Issued: 2014
The labour law consequences of a transfer of a business
- Authors: Abader, Mogamad Shahied
- Date: 2003
- Subjects: Labor laws and legislation -- South Africa , Business enterprises -- Registration and transfer -- South Africa , Labor contract -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:11057 , http://hdl.handle.net/10948/306 , Labor laws and legislation -- South Africa , Business enterprises -- Registration and transfer -- South Africa , Labor contract -- South Africa
- Description: The burden that South African labour law has to bear in relation to the economy is very heavy by international standards. In most industrially developed countries, the economy is strong enough either to provide jobs for most work-seekers or, failing that, an adequate social security system for households without breadwinners in place. In most developing countries with high unemployment rates, the labour law system makes only perfunctory effort to reach out to those facing economic marginalisation. South Africa, essentially a developing country, is not like that. The legal system is strong, works off a firm human rights base, and sets out to grapple with the issues. That is how it should be, but it comes at a price – an oftengraphic exposure of the limits of the law in a stressed society. Businesses operate for profit and survival according to the unsentimental ways of the market, and employees back in a bid to save jobs, lifestyles and livelihoods. The stakeholders use power when they have it, and make claims on the law when they don’t. The legislation and the case law reflect, add to and, to a degree, shape the complexities of these contests, and no more so than in the area of business restructuring.1 The new South Africa has quickly become the destination for foreign investment. The weakness of the rand against the dollar, pound, euro and with the “cost to sell and produce” being so low against these currencies, players on the corporate stage constantly change their make-up and composition. The larger engulfs the smaller, one company buys shares in another, or buys it out entirely, or all or part of its assets, and others are liquidated. In all these situations, employees in South Africa may find themselves with new bosses on the morning after. Under common law employees in this situation were deemed to have been discharged by the former employer, whether or not they have been offered positions in the transformed structure. If they did not want to work under it, they could not be forced to do so. That was because an employment contract was deemed in law to be one of a personal nature that could not be transferred from one employer to another without the employees consent. This research is conducted at an interesting time, when the amendments to the Labour Relations Act 66 of 1995 in respect of the transfer of a business, and in particular section 197, dealing with such matters comes into effect. It is also interesting in the sense that most judgements of the Commission for Conciliation, Mediation and Arbitration (CCMA) and judgements of the Labour Court were moving more or less to a common approach or interpretation of section 197 of the Labour Relations Act 66 of 1995 (hereinafter “the LRA”). Section 197 of the LRA sought to regulate the transfer of a business as a going concern and altered the common law regarding the transfer of a business in two situations – firstly when there is no insolvency, factual or legal, concerned, and secondly in the instance where the transferor is insolvent. The first extreme was when an employer is declared insolvent and the contracts of employment terminated automatically. The second extreme was from the first whereby the employer has to terminate the services of his employees and be liable to pay severance pay in terms of section 1893 of the LRA, which has also been amended along with section 197 of the LRA. It is as if this section was introduced to remedy these extremes. These extremes will be dealt with in detail in this paper. The transfer of goodwill and assets from the seller to the buyer occurs when a business is sold as a going concern. At common law the employees of a business cannot be transferred in the same manner. The Labour Relations Act 66 of 1995 altered this position. By enacting this section the legislature wanted to protect the interest of the employees in such transactions. Whether the legislature has succeeded or not is a matter that will be dealt with in this paper. It is all dependent on the interpretation of this section by the commissioners and judges. By including section 197 in the LRA, the legislature’s intention was to resolve the common law problem where employment contract terminated upon the sale of a business, and this section was intended to be an effective tool for protecting the employment of employees. In order to understand the labour law consequences of the transfer of a business, it is important to understand the provisions of sections 197 and 197A of the Labour Relations Amendment Act 2002. This will be dealt with and each section will be discussed in detail using relevant case law and literature. In considering investing in a South African based company by way of purchasing a share of the company and giving it your own flavour, one has to carefully consider the effects of this transaction. Companies wishing to restructure, outsource, merge or transfer some of its operations will need to understand what the implications of the labour legislation will have on their commercial rationale. Section 197 regulates the employment consequences when a transfer of a business takes place. This is defined to mean the transfer of a business by one employer (the old employer) to another employer (the new employer) as a going concern. Business is defined to include the whole or part of the business, trade undertaking or service. Like the current provision, the new provision referrers to the transfer of a business. It is therefore a wider concept than the sale of a business.4 No attempt is made to define what constitutes a going concern and the controversial issue of whether an outsourcing exercise can constitute a going concern transfer is also not explicitly dealt with. The fact that a business is defined to include a service may be an indication that it was intended to typify outsourcing as a going concern transfer, but this is not necessarily the case.5 The amendments to the Act6 came into effect on 1 August 2002. Sections 197 and 197(A) of the Act consequently seeks to regulate the transfer of a business. These regulations will be dealt with individually and in a format that would make each of the sections in sections 197 and 197(A), easy to understand and interpret. It will also become clear as to what the implications of each of the subsections will have on that commercial rationale. The issues highlighted above will be dealt with detail in this paper giving an overview of the Common Law, the Labour Relations Act 66 of 1995 and the new Labour Relations Amendment Act 2002.
- Full Text:
- Date Issued: 2003
- Authors: Abader, Mogamad Shahied
- Date: 2003
- Subjects: Labor laws and legislation -- South Africa , Business enterprises -- Registration and transfer -- South Africa , Labor contract -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:11057 , http://hdl.handle.net/10948/306 , Labor laws and legislation -- South Africa , Business enterprises -- Registration and transfer -- South Africa , Labor contract -- South Africa
- Description: The burden that South African labour law has to bear in relation to the economy is very heavy by international standards. In most industrially developed countries, the economy is strong enough either to provide jobs for most work-seekers or, failing that, an adequate social security system for households without breadwinners in place. In most developing countries with high unemployment rates, the labour law system makes only perfunctory effort to reach out to those facing economic marginalisation. South Africa, essentially a developing country, is not like that. The legal system is strong, works off a firm human rights base, and sets out to grapple with the issues. That is how it should be, but it comes at a price – an oftengraphic exposure of the limits of the law in a stressed society. Businesses operate for profit and survival according to the unsentimental ways of the market, and employees back in a bid to save jobs, lifestyles and livelihoods. The stakeholders use power when they have it, and make claims on the law when they don’t. The legislation and the case law reflect, add to and, to a degree, shape the complexities of these contests, and no more so than in the area of business restructuring.1 The new South Africa has quickly become the destination for foreign investment. The weakness of the rand against the dollar, pound, euro and with the “cost to sell and produce” being so low against these currencies, players on the corporate stage constantly change their make-up and composition. The larger engulfs the smaller, one company buys shares in another, or buys it out entirely, or all or part of its assets, and others are liquidated. In all these situations, employees in South Africa may find themselves with new bosses on the morning after. Under common law employees in this situation were deemed to have been discharged by the former employer, whether or not they have been offered positions in the transformed structure. If they did not want to work under it, they could not be forced to do so. That was because an employment contract was deemed in law to be one of a personal nature that could not be transferred from one employer to another without the employees consent. This research is conducted at an interesting time, when the amendments to the Labour Relations Act 66 of 1995 in respect of the transfer of a business, and in particular section 197, dealing with such matters comes into effect. It is also interesting in the sense that most judgements of the Commission for Conciliation, Mediation and Arbitration (CCMA) and judgements of the Labour Court were moving more or less to a common approach or interpretation of section 197 of the Labour Relations Act 66 of 1995 (hereinafter “the LRA”). Section 197 of the LRA sought to regulate the transfer of a business as a going concern and altered the common law regarding the transfer of a business in two situations – firstly when there is no insolvency, factual or legal, concerned, and secondly in the instance where the transferor is insolvent. The first extreme was when an employer is declared insolvent and the contracts of employment terminated automatically. The second extreme was from the first whereby the employer has to terminate the services of his employees and be liable to pay severance pay in terms of section 1893 of the LRA, which has also been amended along with section 197 of the LRA. It is as if this section was introduced to remedy these extremes. These extremes will be dealt with in detail in this paper. The transfer of goodwill and assets from the seller to the buyer occurs when a business is sold as a going concern. At common law the employees of a business cannot be transferred in the same manner. The Labour Relations Act 66 of 1995 altered this position. By enacting this section the legislature wanted to protect the interest of the employees in such transactions. Whether the legislature has succeeded or not is a matter that will be dealt with in this paper. It is all dependent on the interpretation of this section by the commissioners and judges. By including section 197 in the LRA, the legislature’s intention was to resolve the common law problem where employment contract terminated upon the sale of a business, and this section was intended to be an effective tool for protecting the employment of employees. In order to understand the labour law consequences of the transfer of a business, it is important to understand the provisions of sections 197 and 197A of the Labour Relations Amendment Act 2002. This will be dealt with and each section will be discussed in detail using relevant case law and literature. In considering investing in a South African based company by way of purchasing a share of the company and giving it your own flavour, one has to carefully consider the effects of this transaction. Companies wishing to restructure, outsource, merge or transfer some of its operations will need to understand what the implications of the labour legislation will have on their commercial rationale. Section 197 regulates the employment consequences when a transfer of a business takes place. This is defined to mean the transfer of a business by one employer (the old employer) to another employer (the new employer) as a going concern. Business is defined to include the whole or part of the business, trade undertaking or service. Like the current provision, the new provision referrers to the transfer of a business. It is therefore a wider concept than the sale of a business.4 No attempt is made to define what constitutes a going concern and the controversial issue of whether an outsourcing exercise can constitute a going concern transfer is also not explicitly dealt with. The fact that a business is defined to include a service may be an indication that it was intended to typify outsourcing as a going concern transfer, but this is not necessarily the case.5 The amendments to the Act6 came into effect on 1 August 2002. Sections 197 and 197(A) of the Act consequently seeks to regulate the transfer of a business. These regulations will be dealt with individually and in a format that would make each of the sections in sections 197 and 197(A), easy to understand and interpret. It will also become clear as to what the implications of each of the subsections will have on that commercial rationale. The issues highlighted above will be dealt with detail in this paper giving an overview of the Common Law, the Labour Relations Act 66 of 1995 and the new Labour Relations Amendment Act 2002.
- Full Text:
- Date Issued: 2003
Investigating the experiences of grade 8 English first additional language learners within an English home language classroom: a case study
- Authors: Abader, Naadirah
- Date: 2018
- Subjects: Language and languages -- Study and teaching -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: http://hdl.handle.net/10948/22662 , vital:30050
- Description: Teaching English home language (EHL) to a classroom of learners who are predominantly from another mother tongue context is a complex process because of the challenges faced by the learners. They struggle to cope with the demands of the EHL classroom, especially when their mother tongue is not recognized within the classroom context, as they move between different linguistic spaces. Despite their diverse identities, English language teachers continue to apply the linguistic approach of English only. This study was aimed at investigating the ways in which the experiences of non-mother tongue speakers in the EHL classroom affect the identities of these learners. Through a qualitative approach using photovoice with learners an attempt was made to probe their experiences of bringing other languages into an English home language classroom. Participants included 33 Grade 8 learners and their four English teachers from Taah High School in Nelson Mandela Bay. The findings have implications for the way in which diverse linguistic learners are taught in an EHL classroom, as the space that caged and rendered learner participants voiceless because they are not allowed to draw on their mother tongue linguistic repertoires, were changed. Learners mentioned that their teachers do not acknowledge the identity that each of them brings to the classroom.
- Full Text:
- Date Issued: 2018
- Authors: Abader, Naadirah
- Date: 2018
- Subjects: Language and languages -- Study and teaching -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: http://hdl.handle.net/10948/22662 , vital:30050
- Description: Teaching English home language (EHL) to a classroom of learners who are predominantly from another mother tongue context is a complex process because of the challenges faced by the learners. They struggle to cope with the demands of the EHL classroom, especially when their mother tongue is not recognized within the classroom context, as they move between different linguistic spaces. Despite their diverse identities, English language teachers continue to apply the linguistic approach of English only. This study was aimed at investigating the ways in which the experiences of non-mother tongue speakers in the EHL classroom affect the identities of these learners. Through a qualitative approach using photovoice with learners an attempt was made to probe their experiences of bringing other languages into an English home language classroom. Participants included 33 Grade 8 learners and their four English teachers from Taah High School in Nelson Mandela Bay. The findings have implications for the way in which diverse linguistic learners are taught in an EHL classroom, as the space that caged and rendered learner participants voiceless because they are not allowed to draw on their mother tongue linguistic repertoires, were changed. Learners mentioned that their teachers do not acknowledge the identity that each of them brings to the classroom.
- Full Text:
- Date Issued: 2018
Cost comparison between repackaging bulk oral solid medicines and purchasing manufacturer-prepared patient-ready packs in the public sector in South Africa
- Authors: Abahamye, Aloysius
- Date: 2014
- Subjects: Packaging -- Cost control , Business logistics -- Costs , Drugs -- Packaging , Manufacturing processes
- Language: English
- Type: Thesis , Masters , MPharm
- Identifier: vital:10163 , http://hdl.handle.net/10948/d1020596 , Drugs -- Packaging
- Description: In an attempt to have medicines available in patient-ready packs (PRPs) prior to the dispensing process, the provincial medicine depots in South Africa have, for many years, been repackaging bulk medicines into PRPs. Notwithstanding the fact that bulk medicine packages may have been the only packages available from the manufacturers to service the Primary Health Care (PHC) sector, the main aim of this process was to ensure that medicines were available in PRPs for dispensing to patients, thus, minimising the time spent on each prescription by the pharmacist, pharmacist’s assistant or Nurse. Currently, some medicines are being procured in PRPs from the manufacturers, whereas others are still procured in bulk packs which must be repackaged into PRPs. After a thorough literature search, it was established that, up until this point in time, no studies have been performed to compare costs of repackaged medicines from bulk packs with costs of medicines procured from manufacturers in PRPs. There was very scanty literature comparing the use of medicines procured either in PRPs or bulk packs. However, literature on cost comparison between repackaging and purchasing of commercially available manufacturer-prepared PRPs was not identified.
- Full Text:
- Date Issued: 2014
- Authors: Abahamye, Aloysius
- Date: 2014
- Subjects: Packaging -- Cost control , Business logistics -- Costs , Drugs -- Packaging , Manufacturing processes
- Language: English
- Type: Thesis , Masters , MPharm
- Identifier: vital:10163 , http://hdl.handle.net/10948/d1020596 , Drugs -- Packaging
- Description: In an attempt to have medicines available in patient-ready packs (PRPs) prior to the dispensing process, the provincial medicine depots in South Africa have, for many years, been repackaging bulk medicines into PRPs. Notwithstanding the fact that bulk medicine packages may have been the only packages available from the manufacturers to service the Primary Health Care (PHC) sector, the main aim of this process was to ensure that medicines were available in PRPs for dispensing to patients, thus, minimising the time spent on each prescription by the pharmacist, pharmacist’s assistant or Nurse. Currently, some medicines are being procured in PRPs from the manufacturers, whereas others are still procured in bulk packs which must be repackaged into PRPs. After a thorough literature search, it was established that, up until this point in time, no studies have been performed to compare costs of repackaged medicines from bulk packs with costs of medicines procured from manufacturers in PRPs. There was very scanty literature comparing the use of medicines procured either in PRPs or bulk packs. However, literature on cost comparison between repackaging and purchasing of commercially available manufacturer-prepared PRPs was not identified.
- Full Text:
- Date Issued: 2014
The androgenic and anabolic effects of pine pollen on Nile tilapia (Oreochromis niloticus)
- Authors: Abaho, Ivan
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431615 , vital:72790
- Description: Access restricted. Expected release date in 2025 , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Abaho, Ivan
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431615 , vital:72790
- Description: Access restricted. Expected release date in 2025 , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
Effects of an eight-week lumbar stabilization exercise programme on selected variables of patients with chronic low back pain:
- Abass, Ademola O, Alli, Abiola R, Olagbegi, Oladapo M, Christie, Candice J, Bolarinde, Olufemi S
- Authors: Abass, Ademola O , Alli, Abiola R , Olagbegi, Oladapo M , Christie, Candice J , Bolarinde, Olufemi S
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/150307 , vital:38966 , DOI: https://doi.org/10.3329/bjms.v19i3.45864
- Description: Lumbar stabilisation exercise has been shown to reduce pain and disability in patients with low back pain but information on its potential benefits in term of back muscle endurance is scarce. This study was aimed at investigating the effects of augmenting conventional physiotherapy with lumbar stabilization exercises on selected variables of patients with non-specific chronic low back pain (NSCLBP).
- Full Text:
- Date Issued: 2020
- Authors: Abass, Ademola O , Alli, Abiola R , Olagbegi, Oladapo M , Christie, Candice J , Bolarinde, Olufemi S
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/150307 , vital:38966 , DOI: https://doi.org/10.3329/bjms.v19i3.45864
- Description: Lumbar stabilisation exercise has been shown to reduce pain and disability in patients with low back pain but information on its potential benefits in term of back muscle endurance is scarce. This study was aimed at investigating the effects of augmenting conventional physiotherapy with lumbar stabilization exercises on selected variables of patients with non-specific chronic low back pain (NSCLBP).
- Full Text:
- Date Issued: 2020
Researcher Profile and List of Publications Dominic Abaver.pdf
- Authors: Abaver, Dominic, Targema
- Identifier: http://hdl.handle.net/11260/4799 , vital:44256
- Description:
Expertise:
Public Health/Medical Microbiology
- Full Text:
- Authors: Abaver, Dominic, Targema
- Identifier: http://hdl.handle.net/11260/4799 , vital:44256
- Description:
Expertise:
Public Health/Medical Microbiology
- Full Text:
Machinability of rapidly solidified aluminium alloy for optical applications
- Authors: Abbas, Abdalla Abbas Said
- Date: 2020
- Subjects: Aluminum alloys , Mechatronics
- Language: English
- Type: Thesis , Masters , MEng
- Identifier: http://hdl.handle.net/10948/45975 , vital:39402
- Description: The production of metal mirrors and critical components for optical devices and aerospace application requires extreme high accuracy and outstanding surface quality. Thus, to achieve such high dimensional accuracies, they are being mainly produced through ultra-high precision machining. Aluminium alloys have been used in the production of components for optics application as well as spaceborne for so many years but with the advancement in technology and demands for a superior material, a new modified grade of aluminium was developed by a rapid solidification process. These grades exhibit a much better mechanical and physical properties while having a finer microstructure. The only downside is the limited research in the correlation of surface roughness and reflectance when single point diamond turned. In this study, rapidly solidified aluminium RSA 905 were used to investigate the effect of varying the cutting parameters on the machined surface finish and its corresponding surface reflectance. The cutting parameters were cutting speed, feed rate and depth of cut. The surface roughness was measured using Taylor Hopson PGI Profilometer while the reflectance factor was measured by using VERTEX 80v Spectrometer. The results were used to develop two predictive models namely; response surface and artificial neural network which have indicated a very high accuracy to the experimental measurements. Finally, the results were very promising for the diamond turning of RSA 905 where it has achieved a very low values of surface roughness and high reflectance in the visual range without the need of any additional production/fabrication steps and to ensure that bi-metallic binding does not take place in extreme low temperatures. Therefore, RSA 905 is a very promising material for optical applications in the visual spectrum.
- Full Text:
- Date Issued: 2020
- Authors: Abbas, Abdalla Abbas Said
- Date: 2020
- Subjects: Aluminum alloys , Mechatronics
- Language: English
- Type: Thesis , Masters , MEng
- Identifier: http://hdl.handle.net/10948/45975 , vital:39402
- Description: The production of metal mirrors and critical components for optical devices and aerospace application requires extreme high accuracy and outstanding surface quality. Thus, to achieve such high dimensional accuracies, they are being mainly produced through ultra-high precision machining. Aluminium alloys have been used in the production of components for optics application as well as spaceborne for so many years but with the advancement in technology and demands for a superior material, a new modified grade of aluminium was developed by a rapid solidification process. These grades exhibit a much better mechanical and physical properties while having a finer microstructure. The only downside is the limited research in the correlation of surface roughness and reflectance when single point diamond turned. In this study, rapidly solidified aluminium RSA 905 were used to investigate the effect of varying the cutting parameters on the machined surface finish and its corresponding surface reflectance. The cutting parameters were cutting speed, feed rate and depth of cut. The surface roughness was measured using Taylor Hopson PGI Profilometer while the reflectance factor was measured by using VERTEX 80v Spectrometer. The results were used to develop two predictive models namely; response surface and artificial neural network which have indicated a very high accuracy to the experimental measurements. Finally, the results were very promising for the diamond turning of RSA 905 where it has achieved a very low values of surface roughness and high reflectance in the visual range without the need of any additional production/fabrication steps and to ensure that bi-metallic binding does not take place in extreme low temperatures. Therefore, RSA 905 is a very promising material for optical applications in the visual spectrum.
- Full Text:
- Date Issued: 2020
Development of an enzyme-synergy based bioreactor system for the beneficiation of apple pomace lignocellulosic waste
- Authors: Abboo, Sagaran
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/315 , vital:19947
- Description: Due to the finite supply of non-renewable fossil fuels, agro-industrial wastes are identified as alternate, renewable sources for energy supply. Large amounts of fruit waste are generated in South Africa due to fruit juice and wine processing from apples, grapes and citrus fruit. Apple pomace is the solid residue that is left over after juice, cider and wine processing and constitutes between 25-30% of the total fruit. On a global scale millions of tonnes of apple pomace are produced; between 2006-2007 over 46 million tonnes were produced. In South Africa a total production of 244 469 tonnes were produced during the 2011- 2012 season. Initially, apple pomace was regarded as a waste by-product used for animal feed and compost in soil, however presently it is considered a source of dietary fiber and natural antioxidants like polyphenols. In addition, apple pomace has a high carbohydrate content and can be enzymatically hydrolysed to produce sugar monomers which, in turn, can be fermented by yeasts to produce bioethanol. The polyphenols present in apple pomace can be used for their health properties, and the bioethanol can be used as a replacement for fossil fuel. Apple pomace is lignocellulosic in nature and consists of hemicellulose, cellulose, lignin and pectin. A combination of enzymes such as cellulases, hemicellulases, pectinases and lignases are required to operate in synergy for the degradation of lignocellulosic biomass. This is due to the recalcitrant nature of lignocellulose. This study investigated the degradation of apple pomace using a combination of commercially obtained enzyme cocktails viz. Viscozyme L , Celluclast 1.5L and Novozyme 188. The commercial enzymes Viscozyme L and Celluclast 1.5L were added in a ratio of 1:1 (50%:50%). The final concentrations of the enzymes were 0.019 mg/ml each. Novozyme 188 was added to provide a final concentration of 0.0024 mg/ml. A novel cost effective 20L bioreactor was designed, constructed and implemented for the degradation of apple pomace to produce value added products. The hydrolysis of the apple pomace was performed initially in 1 L flasks (batch fed) and, once optimized, scaled up to a 20 L bioreactor in batch mode. The bioreactors were operated at room temperature (22 ± 2ºC) and in an unbuffered system. The sugars released were detected and quantified using an optimized validated HPLC method established in this study. The sugars released in the bioreactors were mainly glucose, galactose, arabinose, cellobiose and fructose. The polyphenols released in this study were gallic acid, catechin, epicatechin, chlorogenic acid, rutin and phloridzin, which have a number of health benefits. The simultaneous analyses of the polyphenols were performed using a newly developed and validated HPLC method established in this study. This method was developed to detect nine polyphenols simultaneously. The two HPLC methods developed and validated in this study for the analysis of sugars and polyphenols demonstrated good accuracy, precision, reproducibility, linearity, robustness and sensitivity. Both analytical methods were validated according to the International Convention on Harmonization (ICH). The HPLC parameters for sugar analysis were: refractive index (RI) as the detection mode, the stationary phase was a ligand-exchange sugar column (Shodex SP0810) and an aqueous mobile phase in isocratic mode was used. The HPLC method for polyphenols employed UV diode array detection (DAD) as the detection mode, a reverse phase column as the stationary phase and a mobile phase of consisting of 0.01 M phosphoric acid in water and 100% methanol using gradient elution mode. The highest concentrations of sugars released in the novel 20 L bioreactor with 20% apple pomace (w/v) substrate loading were as follow: glucose (6.5 mg/ml), followed by galactose (2.1 mg/ml), arabinose (1.4 mg/ml), cellobiose (0.7 mg/ml) and fructose (0.5 mg/ml). The amounts of polyphenols released at 20% (w/v) apple pomace substrate were epicatechin (0.01 mg/ml), catechin (0.002 mg/ml), rutin (0.03 mg/ml), chlorogenic acid (0.002 mg/ml) and gallic acid 0.01 (mg/ml). Two mathematical models were developed in this study for kinetic analysis of lignocellulose (apple pomace) hydrolysis in the novel 20 L bioreactor, using the experimental data generated by the above HPLC analyses. The first model, modelling with regression, defines the hydrolysis of the sugars glucose, galactose, cellobiose and arabinose produced in the novel 20 L bioreactor at 5%, 10%, 15% and 20% (w/v) substrate concentrations. The regression model describes the sugars produced in the 20 L bioreactor by minimizing the error of the sugars released by finding a value for K which minimises the function which computes the sum of squares of errors between the solution curves and the data points. The second, more complex, model developed in this study used a system of differential equations model (ODE). This model solved the system by using a numerical method, such as the Runge-Kutta method, then fitted the solution curves to the data. Both models simulated (and had the ability to predict) the production of sugars in the novel 20 L bioreactor for apple pomace hydrolysis. These two models also revealed the time at which the maximum amount of sugars were released, which revealed the optimum time to run the 20 L bioreactor in order to be more cost effective. The optimum time for maximum glucose (the main sugar used in fermentation for biofuel production) release was determined to be around 60 h. The ODE model, in addition, determined the rate at which the substrate became depleted, as well as the rate at which the enzymes became deactivated for the various substrate loadings in the 20 L bioreactor. A third model was developed to determine the optimal running cost of the bioreactor which incorporated the substrate loading and the amount of glucose (g/L) produced. The novel 20 L bioreactor constructed from cost effective materials demonstrated that agro-industrial waste can be converted to value-added products by lignocellolytic enzymes. The sugars released from apple pomace can be used in biofuel production and the polyphenols as food supplements and nutraceuticals for health benefits. This novel study contributes to agro-industrial waste beneficiation via fuel production. In addition, using agro-industrial waste for the generation of value added products (instead of mere disposal) will help prevent environmental pollution.
- Full Text:
- Date Issued: 2016
- Authors: Abboo, Sagaran
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/315 , vital:19947
- Description: Due to the finite supply of non-renewable fossil fuels, agro-industrial wastes are identified as alternate, renewable sources for energy supply. Large amounts of fruit waste are generated in South Africa due to fruit juice and wine processing from apples, grapes and citrus fruit. Apple pomace is the solid residue that is left over after juice, cider and wine processing and constitutes between 25-30% of the total fruit. On a global scale millions of tonnes of apple pomace are produced; between 2006-2007 over 46 million tonnes were produced. In South Africa a total production of 244 469 tonnes were produced during the 2011- 2012 season. Initially, apple pomace was regarded as a waste by-product used for animal feed and compost in soil, however presently it is considered a source of dietary fiber and natural antioxidants like polyphenols. In addition, apple pomace has a high carbohydrate content and can be enzymatically hydrolysed to produce sugar monomers which, in turn, can be fermented by yeasts to produce bioethanol. The polyphenols present in apple pomace can be used for their health properties, and the bioethanol can be used as a replacement for fossil fuel. Apple pomace is lignocellulosic in nature and consists of hemicellulose, cellulose, lignin and pectin. A combination of enzymes such as cellulases, hemicellulases, pectinases and lignases are required to operate in synergy for the degradation of lignocellulosic biomass. This is due to the recalcitrant nature of lignocellulose. This study investigated the degradation of apple pomace using a combination of commercially obtained enzyme cocktails viz. Viscozyme L , Celluclast 1.5L and Novozyme 188. The commercial enzymes Viscozyme L and Celluclast 1.5L were added in a ratio of 1:1 (50%:50%). The final concentrations of the enzymes were 0.019 mg/ml each. Novozyme 188 was added to provide a final concentration of 0.0024 mg/ml. A novel cost effective 20L bioreactor was designed, constructed and implemented for the degradation of apple pomace to produce value added products. The hydrolysis of the apple pomace was performed initially in 1 L flasks (batch fed) and, once optimized, scaled up to a 20 L bioreactor in batch mode. The bioreactors were operated at room temperature (22 ± 2ºC) and in an unbuffered system. The sugars released were detected and quantified using an optimized validated HPLC method established in this study. The sugars released in the bioreactors were mainly glucose, galactose, arabinose, cellobiose and fructose. The polyphenols released in this study were gallic acid, catechin, epicatechin, chlorogenic acid, rutin and phloridzin, which have a number of health benefits. The simultaneous analyses of the polyphenols were performed using a newly developed and validated HPLC method established in this study. This method was developed to detect nine polyphenols simultaneously. The two HPLC methods developed and validated in this study for the analysis of sugars and polyphenols demonstrated good accuracy, precision, reproducibility, linearity, robustness and sensitivity. Both analytical methods were validated according to the International Convention on Harmonization (ICH). The HPLC parameters for sugar analysis were: refractive index (RI) as the detection mode, the stationary phase was a ligand-exchange sugar column (Shodex SP0810) and an aqueous mobile phase in isocratic mode was used. The HPLC method for polyphenols employed UV diode array detection (DAD) as the detection mode, a reverse phase column as the stationary phase and a mobile phase of consisting of 0.01 M phosphoric acid in water and 100% methanol using gradient elution mode. The highest concentrations of sugars released in the novel 20 L bioreactor with 20% apple pomace (w/v) substrate loading were as follow: glucose (6.5 mg/ml), followed by galactose (2.1 mg/ml), arabinose (1.4 mg/ml), cellobiose (0.7 mg/ml) and fructose (0.5 mg/ml). The amounts of polyphenols released at 20% (w/v) apple pomace substrate were epicatechin (0.01 mg/ml), catechin (0.002 mg/ml), rutin (0.03 mg/ml), chlorogenic acid (0.002 mg/ml) and gallic acid 0.01 (mg/ml). Two mathematical models were developed in this study for kinetic analysis of lignocellulose (apple pomace) hydrolysis in the novel 20 L bioreactor, using the experimental data generated by the above HPLC analyses. The first model, modelling with regression, defines the hydrolysis of the sugars glucose, galactose, cellobiose and arabinose produced in the novel 20 L bioreactor at 5%, 10%, 15% and 20% (w/v) substrate concentrations. The regression model describes the sugars produced in the 20 L bioreactor by minimizing the error of the sugars released by finding a value for K which minimises the function which computes the sum of squares of errors between the solution curves and the data points. The second, more complex, model developed in this study used a system of differential equations model (ODE). This model solved the system by using a numerical method, such as the Runge-Kutta method, then fitted the solution curves to the data. Both models simulated (and had the ability to predict) the production of sugars in the novel 20 L bioreactor for apple pomace hydrolysis. These two models also revealed the time at which the maximum amount of sugars were released, which revealed the optimum time to run the 20 L bioreactor in order to be more cost effective. The optimum time for maximum glucose (the main sugar used in fermentation for biofuel production) release was determined to be around 60 h. The ODE model, in addition, determined the rate at which the substrate became depleted, as well as the rate at which the enzymes became deactivated for the various substrate loadings in the 20 L bioreactor. A third model was developed to determine the optimal running cost of the bioreactor which incorporated the substrate loading and the amount of glucose (g/L) produced. The novel 20 L bioreactor constructed from cost effective materials demonstrated that agro-industrial waste can be converted to value-added products by lignocellolytic enzymes. The sugars released from apple pomace can be used in biofuel production and the polyphenols as food supplements and nutraceuticals for health benefits. This novel study contributes to agro-industrial waste beneficiation via fuel production. In addition, using agro-industrial waste for the generation of value added products (instead of mere disposal) will help prevent environmental pollution.
- Full Text:
- Date Issued: 2016
Phenolic compounds in water and the implications for rapid detection of indicator micro-organisms using ß-D-Galactosidase and ß-D-Glucuronidase
- Authors: Abboo, Sagaran
- Date: 2009
- Subjects: Water -- Purification -- Biological treatment , Pollutants -- Biodegradation , Phenol , Organic water pollutants , Water quality biological assessment , Water -- Pollution
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3978 , http://hdl.handle.net/10962/d1004037 , Water -- Purification -- Biological treatment , Pollutants -- Biodegradation , Phenol , Organic water pollutants , Water quality biological assessment , Water -- Pollution
- Description: Faecal contamination in water is detected using appropriate microbial models such as total coliforms, faecal coliforms and E. coli. Βeta-D-Galactosidase (β-GAL) and Beta-D-glucuronidase (β-GUD) are two marker enzymes that are used to test for the presence of total coliforms and E. coli in water samples, respectively. Various assay methods have been developed using chromogenic and fluorogenic substrates. In this study, the chromogenic substrates chlorophenol red β-D-galactopyranoside (CPRG) for β-GAL and p-nitrophenyl-β-D-galactopyranoside (PNPG) for β-GUD were used. Potential problems associated with this approach include interference from other organisms present in the environment (e.g. plants, algae and other bacteria), as well as the presence of certain chemicals, such as phenolic compounds in water. Phenolic compounds are present in the aquatic environment due to their extensive industrial applications. The USA Enviromental Protection Agency (EPA) lists 11 Priority Pollutant Phenols (PPP) due to their high level of toxicity. This study investigated the interfering effects of the eleven PPP found in water on the enzyme activities of both the β-GAL and β-GUD enzyme assays. The presence of these PPP in the β-GAL and β-GUD enzyme assays showed that over and underestimation of activity may occur due to inhibition or activation of these enzymes. Three types of inhibition to enzyme activities were identified from double reciprocal Lineweaver-Burk plots. The inhibition constants (Ki) were determined for all inhibitory phenolic compounds from appropriate secondary plots. Furthermore, this study presented a validated reverse phase high performance liquid chromatography (RP-HPLC) method, developed for the simultaneous detection, separation and determination of all eleven phenolic compounds found in the environment. This method demonstrated good linearity, reproducibility, accuracy and sensitivity. Environmental water samples were collected from rivers, streams, industrial sites and wastewater treatment plant effluent. These samples were extracted and concentrated using a solid phase extraction (SPE) procedure prior to analysis employing the newly developed HPLC method in this study. Seasonal variations on the presence of the PPP in the environment were observed at certain collection sites. The concentrations found were between 0.033 μg/ml for 2,4-dinitrophenol in a running stream to 0.890 mg/ml for pentachlorophenol from an tannery industrial site. These concentrations of phenolic compounds found in these environments were able to interfere with the β-GAL and β-GUD enzyme assays.
- Full Text:
- Date Issued: 2009
- Authors: Abboo, Sagaran
- Date: 2009
- Subjects: Water -- Purification -- Biological treatment , Pollutants -- Biodegradation , Phenol , Organic water pollutants , Water quality biological assessment , Water -- Pollution
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3978 , http://hdl.handle.net/10962/d1004037 , Water -- Purification -- Biological treatment , Pollutants -- Biodegradation , Phenol , Organic water pollutants , Water quality biological assessment , Water -- Pollution
- Description: Faecal contamination in water is detected using appropriate microbial models such as total coliforms, faecal coliforms and E. coli. Βeta-D-Galactosidase (β-GAL) and Beta-D-glucuronidase (β-GUD) are two marker enzymes that are used to test for the presence of total coliforms and E. coli in water samples, respectively. Various assay methods have been developed using chromogenic and fluorogenic substrates. In this study, the chromogenic substrates chlorophenol red β-D-galactopyranoside (CPRG) for β-GAL and p-nitrophenyl-β-D-galactopyranoside (PNPG) for β-GUD were used. Potential problems associated with this approach include interference from other organisms present in the environment (e.g. plants, algae and other bacteria), as well as the presence of certain chemicals, such as phenolic compounds in water. Phenolic compounds are present in the aquatic environment due to their extensive industrial applications. The USA Enviromental Protection Agency (EPA) lists 11 Priority Pollutant Phenols (PPP) due to their high level of toxicity. This study investigated the interfering effects of the eleven PPP found in water on the enzyme activities of both the β-GAL and β-GUD enzyme assays. The presence of these PPP in the β-GAL and β-GUD enzyme assays showed that over and underestimation of activity may occur due to inhibition or activation of these enzymes. Three types of inhibition to enzyme activities were identified from double reciprocal Lineweaver-Burk plots. The inhibition constants (Ki) were determined for all inhibitory phenolic compounds from appropriate secondary plots. Furthermore, this study presented a validated reverse phase high performance liquid chromatography (RP-HPLC) method, developed for the simultaneous detection, separation and determination of all eleven phenolic compounds found in the environment. This method demonstrated good linearity, reproducibility, accuracy and sensitivity. Environmental water samples were collected from rivers, streams, industrial sites and wastewater treatment plant effluent. These samples were extracted and concentrated using a solid phase extraction (SPE) procedure prior to analysis employing the newly developed HPLC method in this study. Seasonal variations on the presence of the PPP in the environment were observed at certain collection sites. The concentrations found were between 0.033 μg/ml for 2,4-dinitrophenol in a running stream to 0.890 mg/ml for pentachlorophenol from an tannery industrial site. These concentrations of phenolic compounds found in these environments were able to interfere with the β-GAL and β-GUD enzyme assays.
- Full Text:
- Date Issued: 2009
A review of asbestos resources
- Authors: Abbott, Paul
- Date: 1983
- Subjects: Asbestos Asbestos -- Geology Silicate minerals Mineralogy Chrysotile
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4910 , http://hdl.handle.net/10962/d1001570
- Full Text:
- Date Issued: 1983
- Authors: Abbott, Paul
- Date: 1983
- Subjects: Asbestos Asbestos -- Geology Silicate minerals Mineralogy Chrysotile
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4910 , http://hdl.handle.net/10962/d1001570
- Full Text:
- Date Issued: 1983
The incorporation of smart production in future factories within the fourth industrial revolution towards 2030
- Authors: Abdoll, Delicia Megan
- Date: 2020
- Subjects: Manufacturing industry
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10948/47521 , vital:40123
- Description: Industry 4.0 is impressively creating a lasting impact on the manufacturing industry and on the industry outlook on the benefits of the implementation of new technology. The concept currently trending entails merging cyber systems, the Internet of Things, and the Internet of Systems all together to construct the revolutionary ‘Smart Factories’. The use of advanced technologies brings about new methodologies to improve the results of manufacturing. Complex activities will be performed by machines equipped in using intelligent information systems and new technology to improve productivity, enhance quality, and reduce costs of manufacturing products. South Africa is at the height of the ‘Fourth Industrial Revolution’ (4IR) which will essentially change the way we work. This innovative rubric of high-tech modernisation is characterised by a merging of technologies from the physical, digital, biological, and neuro-technological spheres. The hesitation over jobs being substituted with co-bots, a robot envisioned to actually cooperate with humans in a communal workplace, is profound. Skills volatility is estimated to affect all industries. There are uncertainties that the underprivileged and non-skilled would be thrust into an even profounder deficiency with the upsurge of the digital age. Organisations regard robotics and modern technology as strategic corporate tools that are utilised to enhance short- and long-term profitability and achieve operating goals. In dissimilarity, the application of robotics and modern technology in the place of work increases labour stability concerns, anxiety of downsizings and terminations within the workforce. The purpose of this research was to heighten the comprehension of smart factories in the manufacturing industry by conclusively embracing a methodical examination of the factors which influence the outlook of those involved concerning smart factory implementation and also of assessing the readiness of the South African manufacturing industry for 4IR towards 2030. The ‘golden thread’ running through the study is the significance of the impact of the 4IR on the workforce and the creation of new jobs for the future, the reskilling of the workforce and the enhancement of capabilities of future factories in embracing the implementation and the incorporation of advanced manufacturing principles in production processes. This must form a substantial consideration in the preparation of the vision of the “Incorporation of smart production in future factories within the fourth industrial revolution towards 2030”. The results of the in-depth analysis of future studies practice and theory in this research study give credibility to the argument that the way in which planning for the future of the 4IR in the South African context is taking place requires insightful adaptation by all stakeholders. The development of new insights through the application of futures studies is vital to this planning process, as is progressively demonstrated in the propensity for present-day business to enable collaborative decisions and strategies that are established on, and informed by, futures studies. This research has attempted to gain insight into the possible future of the implementation of 4IR elements within the future manufacturing factories in South Africa through the creation of four scenarios towards 2030. These are defined as follows: The Fifth Element, which is the ‘best case’ scenario, and to which the country aspires; the ‘worst case’ scenario, in which everything goes badly; the outlier future founded on a surprising, disruptive, emerging matter; and ‘business as usual’ in which no change takes place. The research additionally made efforts to determine the preferred future for the 4IR from a South African perspective, as a base for the Future Vision of the 4IR in the South African manufacturing industry towards 2030. Throughout this study, Inayatullah’s (2008) pillars of futures studies were implemented as a guide in mapping the present and future, further deepening and widening the future through the development of scenarios and, lastly, by transforming the future by narrowing it down to the preferred future. The South African manufacturing sector must select which path to follow in the decisions surrounding the acceptance of the 4IR as the country progresses towards aligning itself with the global players in technology acceptance. Through a unique and innovative approach, the establishment of an atmosphere of trust and the sharing of purpose, values and benefits, a collective Future Vision of the implementing of 4IR elements such as smart production in future factories within South Africa towards 2030, is achievable. All stakeholders must be committed to operating in collaborative partnerships, with government, society, local communities and the workforce all treading boldly together into a sphere of technological, commercial, environmental and social innovation.
- Full Text:
- Date Issued: 2020
- Authors: Abdoll, Delicia Megan
- Date: 2020
- Subjects: Manufacturing industry
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10948/47521 , vital:40123
- Description: Industry 4.0 is impressively creating a lasting impact on the manufacturing industry and on the industry outlook on the benefits of the implementation of new technology. The concept currently trending entails merging cyber systems, the Internet of Things, and the Internet of Systems all together to construct the revolutionary ‘Smart Factories’. The use of advanced technologies brings about new methodologies to improve the results of manufacturing. Complex activities will be performed by machines equipped in using intelligent information systems and new technology to improve productivity, enhance quality, and reduce costs of manufacturing products. South Africa is at the height of the ‘Fourth Industrial Revolution’ (4IR) which will essentially change the way we work. This innovative rubric of high-tech modernisation is characterised by a merging of technologies from the physical, digital, biological, and neuro-technological spheres. The hesitation over jobs being substituted with co-bots, a robot envisioned to actually cooperate with humans in a communal workplace, is profound. Skills volatility is estimated to affect all industries. There are uncertainties that the underprivileged and non-skilled would be thrust into an even profounder deficiency with the upsurge of the digital age. Organisations regard robotics and modern technology as strategic corporate tools that are utilised to enhance short- and long-term profitability and achieve operating goals. In dissimilarity, the application of robotics and modern technology in the place of work increases labour stability concerns, anxiety of downsizings and terminations within the workforce. The purpose of this research was to heighten the comprehension of smart factories in the manufacturing industry by conclusively embracing a methodical examination of the factors which influence the outlook of those involved concerning smart factory implementation and also of assessing the readiness of the South African manufacturing industry for 4IR towards 2030. The ‘golden thread’ running through the study is the significance of the impact of the 4IR on the workforce and the creation of new jobs for the future, the reskilling of the workforce and the enhancement of capabilities of future factories in embracing the implementation and the incorporation of advanced manufacturing principles in production processes. This must form a substantial consideration in the preparation of the vision of the “Incorporation of smart production in future factories within the fourth industrial revolution towards 2030”. The results of the in-depth analysis of future studies practice and theory in this research study give credibility to the argument that the way in which planning for the future of the 4IR in the South African context is taking place requires insightful adaptation by all stakeholders. The development of new insights through the application of futures studies is vital to this planning process, as is progressively demonstrated in the propensity for present-day business to enable collaborative decisions and strategies that are established on, and informed by, futures studies. This research has attempted to gain insight into the possible future of the implementation of 4IR elements within the future manufacturing factories in South Africa through the creation of four scenarios towards 2030. These are defined as follows: The Fifth Element, which is the ‘best case’ scenario, and to which the country aspires; the ‘worst case’ scenario, in which everything goes badly; the outlier future founded on a surprising, disruptive, emerging matter; and ‘business as usual’ in which no change takes place. The research additionally made efforts to determine the preferred future for the 4IR from a South African perspective, as a base for the Future Vision of the 4IR in the South African manufacturing industry towards 2030. Throughout this study, Inayatullah’s (2008) pillars of futures studies were implemented as a guide in mapping the present and future, further deepening and widening the future through the development of scenarios and, lastly, by transforming the future by narrowing it down to the preferred future. The South African manufacturing sector must select which path to follow in the decisions surrounding the acceptance of the 4IR as the country progresses towards aligning itself with the global players in technology acceptance. Through a unique and innovative approach, the establishment of an atmosphere of trust and the sharing of purpose, values and benefits, a collective Future Vision of the implementing of 4IR elements such as smart production in future factories within South Africa towards 2030, is achievable. All stakeholders must be committed to operating in collaborative partnerships, with government, society, local communities and the workforce all treading boldly together into a sphere of technological, commercial, environmental and social innovation.
- Full Text:
- Date Issued: 2020
Molecularly imprinted polymeric materials for adsorptive removal of nitrogen compounds from fuel oils
- Abdul-Quadir, Muhammad Sabiu
- Authors: Abdul-Quadir, Muhammad Sabiu
- Date: 2018
- Subjects: Polymerization , Organonitrogen compounds Nitrogen compounds
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/23426 , vital:30542
- Description: The deleterious effects of refractory polyaromatic hydrocarbons found in fuels such as organonitrogen compounds (quinoline, carbazole and its alkylated derivatives) are such that they emit NOx to the environment when combusted, thereby reducing air quality. These compounds also deactivate the catalyst used during fuel refinement and in catalytic converters of cars. Hydro-denitrogenation (HDN), a process currently being employed in petroleum refineries to eliminate organonitrogen compounds in fuels, is limited in treating these refractory compounds. Hence, this thesis describes the use of two separate complimentary approaches for the removal of organonitrogen compounds in fuel such as oxidative denitrogenation and adsorptive denitrogenation. The catalyzed oxidation of fuel oil model nitrogen containing compound, quinoline to quinoline N-oxide, was conducted under batch and continuous flow microreactor at 70°C by using tert-butylhydroperoxide (t-BuOOH) as oxidant and silica supported V2O5 as catalyst, followed by the selective adsorption of the quinoline N-oxide. An overall conversion of 62% quinoline N-oxide was observed. Quinoline-N-oxide in model fuel was absorbed by employing synthesized molecularly imprinted 2,6-pyridine-polybenzimidazole (2,6-PyPBI) nanofibers, 86% of quinoline-N-oxide was removed to give an adsorption capacity (qe) of 4.8 mg/g. DFT calculations to study the interactions of quinoline-N-oxide vs 2,6-PyPBI indicated that: (i) hydrogen bonding (through amino group of 2,6-PyPBI and oxygen atoms of the quinoline-N-oxide), (ii) pi-pi stacking and (iii) extensive number of van der Waals interactions took place. Several oxygenates from N-compounds were produced, thus, complicating the fuel matrix. Therefore, there is a need to move towards adsorptive denitrogenation. Poly-2-(1H-imidazol-2-yl)-4-phenol (PIMH) imprinted microspheres was prepared by suspension polymerization using 2-(2’-hydroxy-4-ethenylphenyl) imidazole as a functional monomer and ethylene glycol dimethacrylate as a crosslinker in the presence of various organonitrogen compounds (templates) to produce 2-(2’-hydroxy-4-ethenylphenyl) imidazole (PIMH). Imprinted microspheres show selectivity for various target model nitrogen-containing compounds with adsorption capacities of 6.8 ± 0.2 mg/g, 6.3 ± 0.3 mg/g and 5.8 ± 0.3 mg/g for quinoline, pyrimidine and carbazole, respectively. Adsorption selectivity increased in the order of quinoline (αi-r = 136.9) ˃ pyrimidine (αi-r = 126.2) ˃carbazole (αi-r = 86.3), when naphthalene was selected as a reference compound. Though, imprinted microspheres displayed excellent nitrogen compound removal both in model and real fuel, there was a need to improve the adsorbent adsorption capacity for N-compounds in fuel through the fabrication of imprinted nanofibers. Molecularly imprinted poly-2-(1H-imidazol-2-yl)-4-phenol nanofibers was prepared by electrospinning of 2-(2’-hydroxy-4-ethenylphenyl) imidazole (PIMH) in the presence of various organonitrogen compounds. These imprinted nanofibers show selectivity for various target model nitrogen-containing compounds with adsorption capacities of 11.7 ± 0.9 mg/g, 11.9 ± 0.8 mg/g and 11.3 ± 1.1 mg/g for quinoline, pyrimidine and carbazole, respectively. Adsorption selectivity increased in the order of pyrimidine (αi-r = 258.8) ˃ quinoline (αi-r = 235.5) ˃ carbazole (αi-r = 168.2). It further displayed excellent nitrogen removal in real fuel. The use of polybenzimidazole (PBI) nanofibers showed selective adsorption of organonitrogen compounds as imprinted sorbent also displayed high selectivity for their target model nitrogen-containing compounds with adsorption capacities of 11.4 ± 0.4 mg/g, 11.9 ± 0.2 mg/g and 10.9 ± 0.7 mg/g for quinoline, pyrimidine and carbazole respectively. Adsorption selectivity increased in the order of pyrimidine (αi-r = 241.5) ˃ quinoline (αi-r = 237.6) ˃ carbazole (αi-r = 170). Thermodynamic parameters obtained from isothermal titration calorimetry (ITC) revealed that quinoline-PIMH/PBI and pyrimidine-PIMH/PBI interactions are exothermic in nature, while carbazole-PIMH/PBI is endothermic in nature. DFT calculations indicated that π-π interactions/stacking and hydrogen bond interactions took place between N-compounds (carbazole, quinoline and pyrimidine) and adsorbent (PIMH and PBI). A significant reduction in the quantity of nitrogen containing compounds in hydrotreated fuel was observed (peak area reduction) when adsorbents (PIMH and PBI) was employed, however, the complex nature of organonitrogen compounds in fuel complicate the structure/function approach on MIPs for targeting these unwanted compounds.
- Full Text:
- Date Issued: 2018
- Authors: Abdul-Quadir, Muhammad Sabiu
- Date: 2018
- Subjects: Polymerization , Organonitrogen compounds Nitrogen compounds
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/23426 , vital:30542
- Description: The deleterious effects of refractory polyaromatic hydrocarbons found in fuels such as organonitrogen compounds (quinoline, carbazole and its alkylated derivatives) are such that they emit NOx to the environment when combusted, thereby reducing air quality. These compounds also deactivate the catalyst used during fuel refinement and in catalytic converters of cars. Hydro-denitrogenation (HDN), a process currently being employed in petroleum refineries to eliminate organonitrogen compounds in fuels, is limited in treating these refractory compounds. Hence, this thesis describes the use of two separate complimentary approaches for the removal of organonitrogen compounds in fuel such as oxidative denitrogenation and adsorptive denitrogenation. The catalyzed oxidation of fuel oil model nitrogen containing compound, quinoline to quinoline N-oxide, was conducted under batch and continuous flow microreactor at 70°C by using tert-butylhydroperoxide (t-BuOOH) as oxidant and silica supported V2O5 as catalyst, followed by the selective adsorption of the quinoline N-oxide. An overall conversion of 62% quinoline N-oxide was observed. Quinoline-N-oxide in model fuel was absorbed by employing synthesized molecularly imprinted 2,6-pyridine-polybenzimidazole (2,6-PyPBI) nanofibers, 86% of quinoline-N-oxide was removed to give an adsorption capacity (qe) of 4.8 mg/g. DFT calculations to study the interactions of quinoline-N-oxide vs 2,6-PyPBI indicated that: (i) hydrogen bonding (through amino group of 2,6-PyPBI and oxygen atoms of the quinoline-N-oxide), (ii) pi-pi stacking and (iii) extensive number of van der Waals interactions took place. Several oxygenates from N-compounds were produced, thus, complicating the fuel matrix. Therefore, there is a need to move towards adsorptive denitrogenation. Poly-2-(1H-imidazol-2-yl)-4-phenol (PIMH) imprinted microspheres was prepared by suspension polymerization using 2-(2’-hydroxy-4-ethenylphenyl) imidazole as a functional monomer and ethylene glycol dimethacrylate as a crosslinker in the presence of various organonitrogen compounds (templates) to produce 2-(2’-hydroxy-4-ethenylphenyl) imidazole (PIMH). Imprinted microspheres show selectivity for various target model nitrogen-containing compounds with adsorption capacities of 6.8 ± 0.2 mg/g, 6.3 ± 0.3 mg/g and 5.8 ± 0.3 mg/g for quinoline, pyrimidine and carbazole, respectively. Adsorption selectivity increased in the order of quinoline (αi-r = 136.9) ˃ pyrimidine (αi-r = 126.2) ˃carbazole (αi-r = 86.3), when naphthalene was selected as a reference compound. Though, imprinted microspheres displayed excellent nitrogen compound removal both in model and real fuel, there was a need to improve the adsorbent adsorption capacity for N-compounds in fuel through the fabrication of imprinted nanofibers. Molecularly imprinted poly-2-(1H-imidazol-2-yl)-4-phenol nanofibers was prepared by electrospinning of 2-(2’-hydroxy-4-ethenylphenyl) imidazole (PIMH) in the presence of various organonitrogen compounds. These imprinted nanofibers show selectivity for various target model nitrogen-containing compounds with adsorption capacities of 11.7 ± 0.9 mg/g, 11.9 ± 0.8 mg/g and 11.3 ± 1.1 mg/g for quinoline, pyrimidine and carbazole, respectively. Adsorption selectivity increased in the order of pyrimidine (αi-r = 258.8) ˃ quinoline (αi-r = 235.5) ˃ carbazole (αi-r = 168.2). It further displayed excellent nitrogen removal in real fuel. The use of polybenzimidazole (PBI) nanofibers showed selective adsorption of organonitrogen compounds as imprinted sorbent also displayed high selectivity for their target model nitrogen-containing compounds with adsorption capacities of 11.4 ± 0.4 mg/g, 11.9 ± 0.2 mg/g and 10.9 ± 0.7 mg/g for quinoline, pyrimidine and carbazole respectively. Adsorption selectivity increased in the order of pyrimidine (αi-r = 241.5) ˃ quinoline (αi-r = 237.6) ˃ carbazole (αi-r = 170). Thermodynamic parameters obtained from isothermal titration calorimetry (ITC) revealed that quinoline-PIMH/PBI and pyrimidine-PIMH/PBI interactions are exothermic in nature, while carbazole-PIMH/PBI is endothermic in nature. DFT calculations indicated that π-π interactions/stacking and hydrogen bond interactions took place between N-compounds (carbazole, quinoline and pyrimidine) and adsorbent (PIMH and PBI). A significant reduction in the quantity of nitrogen containing compounds in hydrotreated fuel was observed (peak area reduction) when adsorbents (PIMH and PBI) was employed, however, the complex nature of organonitrogen compounds in fuel complicate the structure/function approach on MIPs for targeting these unwanted compounds.
- Full Text:
- Date Issued: 2018
Genetic and biological characterisation of a novel South African Plutella xylostella granulovirus (PlxyGV) isolate
- Authors: Abdulkadir, Fatima
- Date: 2014
- Subjects: Diamondback moth , Diamondback moth -- Control -- South Africa , Plutellidae -- Control -- South Africa , Baculoviruses , Cruciferae -- Diseases and pests -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4113 , http://hdl.handle.net/10962/d1013059
- Description: The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is an important pest of cruciferous crops worldwide. The prolonged use of synthetic chemical insecticides as a primary means of control has resulted in the development of resistance in pest populations. In addition, the pest has also evolved resistance to the bacterial insecticidal protein of Bacillus thuringiensis which is also widely used as a method of control. Baculoviruses are considered as effective alternatives to conventional methods of control when incorporated into integrated pest management (IPM) programmes. These viruses target the larval stages of insects, are generally host-specific and are safe for use in the environment. This study aimed to isolate a baculovirus from a laboratory-reared P. xylostella colony, characterise it genetically and then evaluate its virulence against neonate and fourth instar larvae. A laboratory colony of P. xylostella was established using pupae and asymptomatic larvae collected from a cabbage plantation outside Grahamstown in the Eastern Cape province of South Africa. The colony flourished in the laboratory due to prime conditions and availability of food. The duration of development from egg to adult was determined by observation and imaging of the various life stages. The mean developmental time from egg to adult was observed to be 14.59 ± 0.21 days. The population of the insects increased rapidly in number leading to overcrowding of the insect colony, and hence appearance of larvae with viral symptoms. Occlusion bodies (OBs) were extracted from symptomatic larval cadavers and purified by glycerol gradient centrifugation. Analysis of the purified OBs by transmission electron microscopy revealed the presence of a granulovirus which was named PlxyGV-SA. The virus isolate was genetically characterised by restriction endonuclease analysis of the genomic DNA, and PCR amplification and sequencing of selected viral genes. The complete genome sequence of a Japanese P. xylostella granulovirus isolate, PlxyGV-Japan, has been deposited on the GenBank database providing a reference strain for comparison with DNA profiles and selected gene sequences of PlxyGV-SA. BLAST analysis of the granulin gene confirmed the isolation of a novel South African PlxyGV isolate. Comparison of the restriction profiles of PlxyGV-SA with profiles of PlxyGV-Japan and other documented PlxyGV profiles obtained by agarose gel electrophoresis revealed that PlxyGV-SA is a genetically distinct isolate. The data obtained from the sequencing and alignment of ecdysteroid UDP-glucosyltransferase (egt), late expression factor 8 (lef-8) and late expression factor 9 (lef-9) genes with those of PlxyGV-Japan also showed that PlxyGV-SA is a genetically different isolate. In order to determine the biological activity of PlxyGV-SA against neonate and fourth instar P. xylostella larvae, surface dose bioassays were conducted. The median lethal concentration of the virus required to kill 50% (LC₅₀) and 90% (LC₉₀) of the larvae was estimated by feeding insects with a range of doses. In addition, the time to kill 50% of the larvae (LT₅₀) was determined by feeding insects with the LC₉₀ concentration. Larval mortality was monitored daily until pupation. The data obtained from the dose response assays were subjected to probit analysis using Proban statistical software. The time response was determined using GraphPad Prism software (version 6.0). The LC₅₀ and LC₉₀ values for the neonate larvae were 3.56 × 10⁵ and 1.14 × 10⁷ OBs/ml respectively. The LT₅₀ was determined to be 104 hours. The neonate larvae were found to be more susceptible to infection than the fourth instar larvae with the same virus concentration. The concentrations used for the neonate larvae assay did not have a significant effect on the fourth instar as no mortality was recorded. This is the first study to describe a novel South African PlxyGV isolate and the results suggest that PlxyGV-SA has significant potential for development as an effective biopesticide for the control of P. xylostella in the field.
- Full Text:
- Date Issued: 2014
- Authors: Abdulkadir, Fatima
- Date: 2014
- Subjects: Diamondback moth , Diamondback moth -- Control -- South Africa , Plutellidae -- Control -- South Africa , Baculoviruses , Cruciferae -- Diseases and pests -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4113 , http://hdl.handle.net/10962/d1013059
- Description: The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is an important pest of cruciferous crops worldwide. The prolonged use of synthetic chemical insecticides as a primary means of control has resulted in the development of resistance in pest populations. In addition, the pest has also evolved resistance to the bacterial insecticidal protein of Bacillus thuringiensis which is also widely used as a method of control. Baculoviruses are considered as effective alternatives to conventional methods of control when incorporated into integrated pest management (IPM) programmes. These viruses target the larval stages of insects, are generally host-specific and are safe for use in the environment. This study aimed to isolate a baculovirus from a laboratory-reared P. xylostella colony, characterise it genetically and then evaluate its virulence against neonate and fourth instar larvae. A laboratory colony of P. xylostella was established using pupae and asymptomatic larvae collected from a cabbage plantation outside Grahamstown in the Eastern Cape province of South Africa. The colony flourished in the laboratory due to prime conditions and availability of food. The duration of development from egg to adult was determined by observation and imaging of the various life stages. The mean developmental time from egg to adult was observed to be 14.59 ± 0.21 days. The population of the insects increased rapidly in number leading to overcrowding of the insect colony, and hence appearance of larvae with viral symptoms. Occlusion bodies (OBs) were extracted from symptomatic larval cadavers and purified by glycerol gradient centrifugation. Analysis of the purified OBs by transmission electron microscopy revealed the presence of a granulovirus which was named PlxyGV-SA. The virus isolate was genetically characterised by restriction endonuclease analysis of the genomic DNA, and PCR amplification and sequencing of selected viral genes. The complete genome sequence of a Japanese P. xylostella granulovirus isolate, PlxyGV-Japan, has been deposited on the GenBank database providing a reference strain for comparison with DNA profiles and selected gene sequences of PlxyGV-SA. BLAST analysis of the granulin gene confirmed the isolation of a novel South African PlxyGV isolate. Comparison of the restriction profiles of PlxyGV-SA with profiles of PlxyGV-Japan and other documented PlxyGV profiles obtained by agarose gel electrophoresis revealed that PlxyGV-SA is a genetically distinct isolate. The data obtained from the sequencing and alignment of ecdysteroid UDP-glucosyltransferase (egt), late expression factor 8 (lef-8) and late expression factor 9 (lef-9) genes with those of PlxyGV-Japan also showed that PlxyGV-SA is a genetically different isolate. In order to determine the biological activity of PlxyGV-SA against neonate and fourth instar P. xylostella larvae, surface dose bioassays were conducted. The median lethal concentration of the virus required to kill 50% (LC₅₀) and 90% (LC₉₀) of the larvae was estimated by feeding insects with a range of doses. In addition, the time to kill 50% of the larvae (LT₅₀) was determined by feeding insects with the LC₉₀ concentration. Larval mortality was monitored daily until pupation. The data obtained from the dose response assays were subjected to probit analysis using Proban statistical software. The time response was determined using GraphPad Prism software (version 6.0). The LC₅₀ and LC₉₀ values for the neonate larvae were 3.56 × 10⁵ and 1.14 × 10⁷ OBs/ml respectively. The LT₅₀ was determined to be 104 hours. The neonate larvae were found to be more susceptible to infection than the fourth instar larvae with the same virus concentration. The concentrations used for the neonate larvae assay did not have a significant effect on the fourth instar as no mortality was recorded. This is the first study to describe a novel South African PlxyGV isolate and the results suggest that PlxyGV-SA has significant potential for development as an effective biopesticide for the control of P. xylostella in the field.
- Full Text:
- Date Issued: 2014
Genetic and biological characterisation of a novel Plutella xylostella granulovirus, PlxyGV-SA
- Abdulkadir, Fatima, Knox, Caroline M, Marsberg, Tamryn, Hill, Martin P, Moore, Sean D
- Authors: Abdulkadir, Fatima , Knox, Caroline M , Marsberg, Tamryn , Hill, Martin P , Moore, Sean D
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/417971 , vital:71498 , xlink:href="https://doi.org/10.1007/s10526-015-9666-3"
- Description: Plutella xylostella granulovirus (PlxyGV) has been isolated from insect populations in many countries and is considered a potential biopesticide for sustainable control of P. xylostella (L.) (Lepidoptera: Plutellidae). Several PlxyGV isolates have been genetically characterised, and the full genome sequence of PlxyGV-Japan is available for comparison with novel isolates. A South African PlxyGV was recently recovered from an overcrowded laboratory P. xylostella colony and identified as a genetically distinct isolate by sequencing of the granulin gene and restriction endonuclease (REN) analysis of genomic DNA. In this report, PlxyGV-SA was further characterised by PCR amplification and sequencing of egt, lef-8 and lef-9 genes, and several amino acid substitutions were observed. The PstI REN profile of PlxyGV-SA was different from that of PlxyGV-Japan in terms of band size and number, thereby confirming its novel genetic identity. Surface dose bioassays showed that PlxyGV-SA is pathogenic to neonate but not late instar larvae at the same and higher virus doses, indicating that a biopesticide should be targeted at early larval stages in the field.
- Full Text:
- Date Issued: 2015
- Authors: Abdulkadir, Fatima , Knox, Caroline M , Marsberg, Tamryn , Hill, Martin P , Moore, Sean D
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/417971 , vital:71498 , xlink:href="https://doi.org/10.1007/s10526-015-9666-3"
- Description: Plutella xylostella granulovirus (PlxyGV) has been isolated from insect populations in many countries and is considered a potential biopesticide for sustainable control of P. xylostella (L.) (Lepidoptera: Plutellidae). Several PlxyGV isolates have been genetically characterised, and the full genome sequence of PlxyGV-Japan is available for comparison with novel isolates. A South African PlxyGV was recently recovered from an overcrowded laboratory P. xylostella colony and identified as a genetically distinct isolate by sequencing of the granulin gene and restriction endonuclease (REN) analysis of genomic DNA. In this report, PlxyGV-SA was further characterised by PCR amplification and sequencing of egt, lef-8 and lef-9 genes, and several amino acid substitutions were observed. The PstI REN profile of PlxyGV-SA was different from that of PlxyGV-Japan in terms of band size and number, thereby confirming its novel genetic identity. Surface dose bioassays showed that PlxyGV-SA is pathogenic to neonate but not late instar larvae at the same and higher virus doses, indicating that a biopesticide should be targeted at early larval stages in the field.
- Full Text:
- Date Issued: 2015
Morphological and genetic characterization of a South African Plutella xylostella granulovirus (plxy GV) isolate
- Abdulkadir, Fatima, Marsberg, Tamryn, Knox, Caroline M, Hill, Martin P, Moore, Sean D
- Authors: Abdulkadir, Fatima , Marsberg, Tamryn , Knox, Caroline M , Hill, Martin P , Moore, Sean D
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/406117 , vital:70240 , xlink:href="https://hdl.handle.net/10520/EJC132828"
- Description: Plutella xylostella (L.) (Lepidoptera: Plutellidae), also known as diamondback moth, is a destructive insect pest of cruciferous crops (Talekar and Shelton 1993; Shelton 2004). The pest occurs wherever its host plants are cultivated and the global annual cost of damage and control is estimated to be US$4-5 billion (Zalucki et al. 2012). The extensive use of synthetic pesticides for control combined with the high fecundity of P. xylostella has resulted in the pest developing resistance to nearly all classes of insecticides (Grzywacz et al. 2009). Moreover, these chemicals have negative environmental implications and may affect non-target species, some of which are natural enemies of the pest.
- Full Text:
- Date Issued: 2013
- Authors: Abdulkadir, Fatima , Marsberg, Tamryn , Knox, Caroline M , Hill, Martin P , Moore, Sean D
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/406117 , vital:70240 , xlink:href="https://hdl.handle.net/10520/EJC132828"
- Description: Plutella xylostella (L.) (Lepidoptera: Plutellidae), also known as diamondback moth, is a destructive insect pest of cruciferous crops (Talekar and Shelton 1993; Shelton 2004). The pest occurs wherever its host plants are cultivated and the global annual cost of damage and control is estimated to be US$4-5 billion (Zalucki et al. 2012). The extensive use of synthetic pesticides for control combined with the high fecundity of P. xylostella has resulted in the pest developing resistance to nearly all classes of insecticides (Grzywacz et al. 2009). Moreover, these chemicals have negative environmental implications and may affect non-target species, some of which are natural enemies of the pest.
- Full Text:
- Date Issued: 2013