Bacterial colonisation and degradation of geologically weathered and discard coal
- Authors: Olawale, Jacob Taiwo
- Date: 2018
- Subjects: Coal mine waste , Coal -- Biodegradation , Coal mines and mining -- Environmental aspects , Land degradation , Electron microscopy , Extracellular polymeric substances , Flagella (Microbiology) , Fourier transform infrared spectroscopy , Microbiologically influenced corrosion
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61625 , vital:28043
- Description: Bacterial beneficiation of low-grade coal, coal discard, and waste has the potential to mitigate land degradation, water and soil pollution and, be a strategy for mining companies to responsibly extract and process coal with environmental sustainability. This study investigated the colonisation and biodegradation or depolymerisation of coal discard and geologically weathered coal by selected strains of bacteria, and an attempt has been made to describe the mechanisms associated with colonisation and biodegradation of this carbonaceous material. Ten bacterial strains, Bacillus strain ECCN 18b, Citrobacter strain ECCN 19b, Proteus strain ECCN 20b, Exiguobacterium strain ECCN 21b, Microbacterium strain ECCN 22b, Proteus strain ECCN 23b, Serratia strain ECCN 24b, Escherichia strain ECCN 25b, Bacillus strain ECCN 26b and Bacillus strain ECCN 41b, isolated from diesel-contaminated soil and coal slurry and identified using DNA sequencing, were rescreened and their coal biodegradation potential ranked. The ranking of the bacterial strains was undertaken using several indicators including; formation of brown halos on the plate culture (solid), change in colour intensity of the medium in liquid culture, change in culture media pH, and an increase in absorbance at 280nm and 450nm. Although, all the ten strains showed evidence of biodegradation of coal discard and geologically weathered coal based on the ranking employed, and the three strains considered the best candidates were Citrobacter strain ECCN 19b, Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b. The actions of the three bacterial strains were further studied and characterised in relation to coal degradation. Electron microscopy revealed that Citrobacter strain ECCN 19b, Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b attached to the surface of coal discard and geologically weathered coal by a process that appeared to involve extracellular polymeric substances (EPS), and flagella. The presence of flagella for Citrobacter strain ECCN 19b and Serratia strain ECCN 24b was confirmed by transmission electron microscopy. Bacterial degradation of coal discard and geologically weathered coal by these selected strains resulted in the release of soluble and insoluble products. Ultraviolet/ visible spectrophotometric (UV/VIS) analysis revealed that the soluble products resembled humic acid-like substances, which was confirmed following Fourier Transform Infrared (FTIR) spectroscopy. Analysis revealed that the coal-derived humic acid-like substances were similar to commercial humic acid extracted from bituminous coal. Elemental analysis of the insoluble product residue after bacterial biodegradation revealed the modification of the chemical compositions of the coal discard and geologically weathered coal substrates. Characterisation of the functional groups of the insoluble product using FTIR spectroscopy indicated changes, with the appearance of new peaks at 1737cm-1, 1366cm-1, 1228cm-1, and 1216cm-1 characteristic of aldehyde, ketones, carboxylic acids, esters, amines, and alkanes. Broad spectra regions of 3500 -3200cm-1, characteristic of alcohol and phenol, were also observed. Together, these results were taken as evidence for increased oxidation of the coal substrates, presumably as a consequence of bacterial catalysed biodegradation of coal discard and geologically weathered coal. During bacterial degradation of coal discard and geologically weathered coal, strains produced extracellular protein, which was detected and further investigated using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS- PAGE). At least three protein bands with molecular mass 53 kDa, 72 kDa, and 82 kDa were common to the three bacterial strains. Following ammonium sulphate precipitation and gel filtration chromatography, additional bands with molecular mass 16 kDa, 33 kDa, 37 kDa, and 43 kDa were detected. An extracellular laccase activity was detected in cultures of Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b. Cytochrome P450 activity was detected in all the bacterial strains in the presence of both coal discard and geologically weathered coal. This is the first time that cytochrome P450 activity has been reported following exposure of these three bacterial strains to a coal substrate. Overall, this research has successfully demonstrated the partial degradation of coal discard and geologically weathered coal by Citrobacter strain ECCN 19b, Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b and the release of humic acid-like substances. Thus, the biodegradation process involved adherence to and growth of the bacteria on the surface of coal substrate and appeared to require the formation of alkaline substances and the combined activities of extracellular LAC and cytochrome P450. Since bacterial degradation of low-grade coal and discard appears to be viable, the bacteria isolated in this study can potentially be used either for conversion of discard into valuable chemicals or to mitigate the deleterious effects of stockpiled coal discard on the environment.
- Full Text:
- Date Issued: 2018
- Authors: Olawale, Jacob Taiwo
- Date: 2018
- Subjects: Coal mine waste , Coal -- Biodegradation , Coal mines and mining -- Environmental aspects , Land degradation , Electron microscopy , Extracellular polymeric substances , Flagella (Microbiology) , Fourier transform infrared spectroscopy , Microbiologically influenced corrosion
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61625 , vital:28043
- Description: Bacterial beneficiation of low-grade coal, coal discard, and waste has the potential to mitigate land degradation, water and soil pollution and, be a strategy for mining companies to responsibly extract and process coal with environmental sustainability. This study investigated the colonisation and biodegradation or depolymerisation of coal discard and geologically weathered coal by selected strains of bacteria, and an attempt has been made to describe the mechanisms associated with colonisation and biodegradation of this carbonaceous material. Ten bacterial strains, Bacillus strain ECCN 18b, Citrobacter strain ECCN 19b, Proteus strain ECCN 20b, Exiguobacterium strain ECCN 21b, Microbacterium strain ECCN 22b, Proteus strain ECCN 23b, Serratia strain ECCN 24b, Escherichia strain ECCN 25b, Bacillus strain ECCN 26b and Bacillus strain ECCN 41b, isolated from diesel-contaminated soil and coal slurry and identified using DNA sequencing, were rescreened and their coal biodegradation potential ranked. The ranking of the bacterial strains was undertaken using several indicators including; formation of brown halos on the plate culture (solid), change in colour intensity of the medium in liquid culture, change in culture media pH, and an increase in absorbance at 280nm and 450nm. Although, all the ten strains showed evidence of biodegradation of coal discard and geologically weathered coal based on the ranking employed, and the three strains considered the best candidates were Citrobacter strain ECCN 19b, Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b. The actions of the three bacterial strains were further studied and characterised in relation to coal degradation. Electron microscopy revealed that Citrobacter strain ECCN 19b, Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b attached to the surface of coal discard and geologically weathered coal by a process that appeared to involve extracellular polymeric substances (EPS), and flagella. The presence of flagella for Citrobacter strain ECCN 19b and Serratia strain ECCN 24b was confirmed by transmission electron microscopy. Bacterial degradation of coal discard and geologically weathered coal by these selected strains resulted in the release of soluble and insoluble products. Ultraviolet/ visible spectrophotometric (UV/VIS) analysis revealed that the soluble products resembled humic acid-like substances, which was confirmed following Fourier Transform Infrared (FTIR) spectroscopy. Analysis revealed that the coal-derived humic acid-like substances were similar to commercial humic acid extracted from bituminous coal. Elemental analysis of the insoluble product residue after bacterial biodegradation revealed the modification of the chemical compositions of the coal discard and geologically weathered coal substrates. Characterisation of the functional groups of the insoluble product using FTIR spectroscopy indicated changes, with the appearance of new peaks at 1737cm-1, 1366cm-1, 1228cm-1, and 1216cm-1 characteristic of aldehyde, ketones, carboxylic acids, esters, amines, and alkanes. Broad spectra regions of 3500 -3200cm-1, characteristic of alcohol and phenol, were also observed. Together, these results were taken as evidence for increased oxidation of the coal substrates, presumably as a consequence of bacterial catalysed biodegradation of coal discard and geologically weathered coal. During bacterial degradation of coal discard and geologically weathered coal, strains produced extracellular protein, which was detected and further investigated using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS- PAGE). At least three protein bands with molecular mass 53 kDa, 72 kDa, and 82 kDa were common to the three bacterial strains. Following ammonium sulphate precipitation and gel filtration chromatography, additional bands with molecular mass 16 kDa, 33 kDa, 37 kDa, and 43 kDa were detected. An extracellular laccase activity was detected in cultures of Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b. Cytochrome P450 activity was detected in all the bacterial strains in the presence of both coal discard and geologically weathered coal. This is the first time that cytochrome P450 activity has been reported following exposure of these three bacterial strains to a coal substrate. Overall, this research has successfully demonstrated the partial degradation of coal discard and geologically weathered coal by Citrobacter strain ECCN 19b, Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b and the release of humic acid-like substances. Thus, the biodegradation process involved adherence to and growth of the bacteria on the surface of coal substrate and appeared to require the formation of alkaline substances and the combined activities of extracellular LAC and cytochrome P450. Since bacterial degradation of low-grade coal and discard appears to be viable, the bacteria isolated in this study can potentially be used either for conversion of discard into valuable chemicals or to mitigate the deleterious effects of stockpiled coal discard on the environment.
- Full Text:
- Date Issued: 2018
A thematic analysis of the challenges experienced by those living with tuberculosis
- Authors: Walaza, Robert Letsholo
- Date: 2018
- Subjects: Tuberculosis Patients South Africa , Tuberculosis Social aspects , South Africa Social conditions , Poor Health and hygiene South Africa , Poor Medical care South Africa , Social medicine South Africa
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/61850 , vital:28068
- Description: Friedrich Engels (1820-1895) in his study, The condition of the working class in England, argued that the cause of illness and death amongst the working class was due to their living conditions such as poor housing, over-crowding, poor sanitation, food shortage, low paying jobs and a lack of material resources. The objective of the study was to understand the experiences of six South African individuals who have shared their experiences and challenges of living with TB on the TB&ME blog, and to show how TB is linked to the living conditions of these individuals. The study found that the challenges experienced by TB patient bloggers are of a social nature and confirms Engel’s study findings on the conditions of the working class in England. For example, a disease such as TB has a direct association with the living conditions of people, especially the poor. Thus, socio economic status of TB patient bloggers plays a role in the escalation of their ill health. Further, the study found that gender is central in understanding non-compliance to treatment. This is significant as it highlights the need to not only focus on issues of socioeconomics, but gender issues in fighting TB. Despite the negative consequences associated with living with TB, the bloggers have noted that the support from loved ones and other stakeholders in the fight against TB alleviates the challenges inherent in living with TB.
- Full Text:
- Date Issued: 2018
- Authors: Walaza, Robert Letsholo
- Date: 2018
- Subjects: Tuberculosis Patients South Africa , Tuberculosis Social aspects , South Africa Social conditions , Poor Health and hygiene South Africa , Poor Medical care South Africa , Social medicine South Africa
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/61850 , vital:28068
- Description: Friedrich Engels (1820-1895) in his study, The condition of the working class in England, argued that the cause of illness and death amongst the working class was due to their living conditions such as poor housing, over-crowding, poor sanitation, food shortage, low paying jobs and a lack of material resources. The objective of the study was to understand the experiences of six South African individuals who have shared their experiences and challenges of living with TB on the TB&ME blog, and to show how TB is linked to the living conditions of these individuals. The study found that the challenges experienced by TB patient bloggers are of a social nature and confirms Engel’s study findings on the conditions of the working class in England. For example, a disease such as TB has a direct association with the living conditions of people, especially the poor. Thus, socio economic status of TB patient bloggers plays a role in the escalation of their ill health. Further, the study found that gender is central in understanding non-compliance to treatment. This is significant as it highlights the need to not only focus on issues of socioeconomics, but gender issues in fighting TB. Despite the negative consequences associated with living with TB, the bloggers have noted that the support from loved ones and other stakeholders in the fight against TB alleviates the challenges inherent in living with TB.
- Full Text:
- Date Issued: 2018
Long-term analysis of ionospheric response during geomagnetic storms in mid, low and equatorial latitudes
- Matamba, Tshimangadzo Merline
- Authors: Matamba, Tshimangadzo Merline
- Date: 2018
- Subjects: Ionospheric storms , Coronal mass ejections , Corotating interaction regions , Solar flares , Global Positioning System , Ionospheric critical frequencies , Equatorial Ionization Anomaly (EIA)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63991 , vital:28517
- Description: Understanding changes in the ionosphere is important for High Frequency (HF) communications and navigation systems. Ionospheric storms are the disturbances in the Earth’s upper atmosphere due to solar activities such as Coronal Mass Ejections (CMEs), Corotating interaction Regions (CIRs) and solar flares. This thesis reports for the first time on an investigation of ionospheric response to great geomagnetic storms (Disturbance storm time, Dst ≤ −350 nT) that occurred during solar cycle 23. The storm periods analysed were 29 March - 02 April 2001, 27 - 31 October 2003, 18 - 23 November 2003 and 06 - 11 November 2004. Global Navigation Satellite System (GNSS), Total Electron Content (TEC) and ionosonde critical frequency of F2 layer (foF2) data over northern hemisphere (European sector) and southern hemisphere (African sector) mid-latitudes were used to study the ionospheric responses within 15E° - 40°E longitude and ±31°- ±46° geomagnetic latitude. Mid-latitude regions within the same longitude sector in both hemispheres were selected in order to assess the contribution of the low latitude changes especially the expansion of Equatorial Ionization Anomaly (EIA) also known as the dayside ionospheric super-fountain effect during these storms. In all storm periods, both negative and positive ionospheric responses were observed in both hemispheres. Negative ionospheric responses were mainly due to changes in neutral composition, while the expansion of the EIA led to pronounced positive ionospheric storm effect at mid-latitudes for some storm periods. In other cases (e.g 29 October 2003), Prompt Penetration Electric Fields (PPEF), EIA expansion and large scale Traveling Ionospheric Disturbances (TIDs) were found to be present during the positive storm effect at mid-latitudes in both hemispheres. An increase in TEC on the 28 October 2003 was because of the large solar flare with previously determined intensity of X45± 5. A further report on statistical analysis of ionospheric storm effects due to Corotating Interaction Region (CIR)- and Coronal Mass Ejection (CME)-driven storms was performed. The storm periods analyzed occurred during the period 2001 - 2015 which covers part of solar cycles 23 and 24. Dst≤ -30 nT and Kp≥ 3 indices were used to identify the storm periods considered. Ionospheric TEC derived from IGS stations that lie within 30°E - 40°E geographic longitude in mid, low and equatorial latitude over the African sector were used. The statistical analysis of ionospheric storm effects were compared over mid, low and equatorial latitudes in the African sector for the first time. Positive ionospheric storm effects were more prevalent during CME-driven and CIR-driven over all stations considered in this study. Negative ionospheric storm effects occurred only during CME-driven storms over mid-latitude stations and were more prevalent in summer. The other interesting finding is that for the stations considered over mid-, low, and equatorial latitudes, negative-positive ionospheric responses were only observed over low and equatorial latitudes. A significant number of cases where the electron density changes remained within the background variability during storm conditions were observed over the low latitude stations compared to other latitude regions.
- Full Text:
- Date Issued: 2018
- Authors: Matamba, Tshimangadzo Merline
- Date: 2018
- Subjects: Ionospheric storms , Coronal mass ejections , Corotating interaction regions , Solar flares , Global Positioning System , Ionospheric critical frequencies , Equatorial Ionization Anomaly (EIA)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63991 , vital:28517
- Description: Understanding changes in the ionosphere is important for High Frequency (HF) communications and navigation systems. Ionospheric storms are the disturbances in the Earth’s upper atmosphere due to solar activities such as Coronal Mass Ejections (CMEs), Corotating interaction Regions (CIRs) and solar flares. This thesis reports for the first time on an investigation of ionospheric response to great geomagnetic storms (Disturbance storm time, Dst ≤ −350 nT) that occurred during solar cycle 23. The storm periods analysed were 29 March - 02 April 2001, 27 - 31 October 2003, 18 - 23 November 2003 and 06 - 11 November 2004. Global Navigation Satellite System (GNSS), Total Electron Content (TEC) and ionosonde critical frequency of F2 layer (foF2) data over northern hemisphere (European sector) and southern hemisphere (African sector) mid-latitudes were used to study the ionospheric responses within 15E° - 40°E longitude and ±31°- ±46° geomagnetic latitude. Mid-latitude regions within the same longitude sector in both hemispheres were selected in order to assess the contribution of the low latitude changes especially the expansion of Equatorial Ionization Anomaly (EIA) also known as the dayside ionospheric super-fountain effect during these storms. In all storm periods, both negative and positive ionospheric responses were observed in both hemispheres. Negative ionospheric responses were mainly due to changes in neutral composition, while the expansion of the EIA led to pronounced positive ionospheric storm effect at mid-latitudes for some storm periods. In other cases (e.g 29 October 2003), Prompt Penetration Electric Fields (PPEF), EIA expansion and large scale Traveling Ionospheric Disturbances (TIDs) were found to be present during the positive storm effect at mid-latitudes in both hemispheres. An increase in TEC on the 28 October 2003 was because of the large solar flare with previously determined intensity of X45± 5. A further report on statistical analysis of ionospheric storm effects due to Corotating Interaction Region (CIR)- and Coronal Mass Ejection (CME)-driven storms was performed. The storm periods analyzed occurred during the period 2001 - 2015 which covers part of solar cycles 23 and 24. Dst≤ -30 nT and Kp≥ 3 indices were used to identify the storm periods considered. Ionospheric TEC derived from IGS stations that lie within 30°E - 40°E geographic longitude in mid, low and equatorial latitude over the African sector were used. The statistical analysis of ionospheric storm effects were compared over mid, low and equatorial latitudes in the African sector for the first time. Positive ionospheric storm effects were more prevalent during CME-driven and CIR-driven over all stations considered in this study. Negative ionospheric storm effects occurred only during CME-driven storms over mid-latitude stations and were more prevalent in summer. The other interesting finding is that for the stations considered over mid-, low, and equatorial latitudes, negative-positive ionospheric responses were only observed over low and equatorial latitudes. A significant number of cases where the electron density changes remained within the background variability during storm conditions were observed over the low latitude stations compared to other latitude regions.
- Full Text:
- Date Issued: 2018
The Kenya National Human Rights Commission and the promotion, protection and monitoring of socio-economic rights in Kenya
- Khayundi, Francis Bulimo Mapati
- Authors: Khayundi, Francis Bulimo Mapati
- Date: 2018
- Subjects: Kenya Human Rights Commission , Economic rights -- Kenya , Social rights -- Kenya , Kenya -- Economic conditions , Kenya -- Social conditions , Kenya -- Politics and government
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60413 , vital:27777
- Description: The promulgation of the 2010 Constitution of Kenya introduced socio-economic rights (SERs) amid widespread poverty and rising inequality. This study seeks to answer the overarching question, what role can the Kenya National Commission on Human Rights (KNCHR) play in promoting, protecting and monitoring SERs in Kenya? Further research questions included whether the KNCHR has the requisite powers to perform its mandate and what lessons could be learned from the South African context. The research sought to understand how the local context affects the ability of KNCHR to carry out its mandate. Likewise, it analyses some of the contributions KNCHR has made in the promotion and protection of SERs while identifying the challenges the Commission faces in carrying out its mandate. Several methodologies were utilised to answer the research questions above. The methodologies included the doctrinal method, analysis of secondary sources and interviews with key informants. A comparative legal research methodology was also employed, with the SAHRC being used as a case study on how NHRIs can promote, protect and monitor SERs. The findings from the research argue that the Paris Principles provide the minimum guidelines on the establishment of NHRIs. Compliance with these Principles has not necessarily guaranteed the effectives of NHRIs. Any assessment of an NHRI should be based on its performance and legitimacy considering the local factors obtaining within its jurisdiction. The domestic protection and judicial enforcement of human rights in Kenya, though crucial to the realisation of SERs, has been fraught with challenges. These challenges have meant that the realisation of SERs has been curtailed and necessitated complementary institutions for human rights to be realised. Given the country’s constitutional architecture, the KNCHR was one such institution that could complement the role of the judiciary given its wide mandate. With SERs a new feature of the 2010 Constitution, the KNCHR had to find ways to promote SERs in the country considering the local peculiarities such as poverty, a highly political climate and lack of political goodwill from the legislature and executive sometime characterised by open hostility. These challenges and the new nature of these rights called for a comparative study with the SAHRC given some similarities between the two jurisdictions. The SAHRC provided valuable lessons having had more experience in dealing with SERs while navigating similar challenges the KNCHR faced or might face. The findings of the research prompted recommendations directed at the KNCHR and other stakeholders, specifically the legislature and executive on how to address the challenges curtailing the performance of the KNCHR in general and particularly ways in which the Commission could go about in promoting, protecting and monitoring SERs.
- Full Text:
- Date Issued: 2018
- Authors: Khayundi, Francis Bulimo Mapati
- Date: 2018
- Subjects: Kenya Human Rights Commission , Economic rights -- Kenya , Social rights -- Kenya , Kenya -- Economic conditions , Kenya -- Social conditions , Kenya -- Politics and government
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60413 , vital:27777
- Description: The promulgation of the 2010 Constitution of Kenya introduced socio-economic rights (SERs) amid widespread poverty and rising inequality. This study seeks to answer the overarching question, what role can the Kenya National Commission on Human Rights (KNCHR) play in promoting, protecting and monitoring SERs in Kenya? Further research questions included whether the KNCHR has the requisite powers to perform its mandate and what lessons could be learned from the South African context. The research sought to understand how the local context affects the ability of KNCHR to carry out its mandate. Likewise, it analyses some of the contributions KNCHR has made in the promotion and protection of SERs while identifying the challenges the Commission faces in carrying out its mandate. Several methodologies were utilised to answer the research questions above. The methodologies included the doctrinal method, analysis of secondary sources and interviews with key informants. A comparative legal research methodology was also employed, with the SAHRC being used as a case study on how NHRIs can promote, protect and monitor SERs. The findings from the research argue that the Paris Principles provide the minimum guidelines on the establishment of NHRIs. Compliance with these Principles has not necessarily guaranteed the effectives of NHRIs. Any assessment of an NHRI should be based on its performance and legitimacy considering the local factors obtaining within its jurisdiction. The domestic protection and judicial enforcement of human rights in Kenya, though crucial to the realisation of SERs, has been fraught with challenges. These challenges have meant that the realisation of SERs has been curtailed and necessitated complementary institutions for human rights to be realised. Given the country’s constitutional architecture, the KNCHR was one such institution that could complement the role of the judiciary given its wide mandate. With SERs a new feature of the 2010 Constitution, the KNCHR had to find ways to promote SERs in the country considering the local peculiarities such as poverty, a highly political climate and lack of political goodwill from the legislature and executive sometime characterised by open hostility. These challenges and the new nature of these rights called for a comparative study with the SAHRC given some similarities between the two jurisdictions. The SAHRC provided valuable lessons having had more experience in dealing with SERs while navigating similar challenges the KNCHR faced or might face. The findings of the research prompted recommendations directed at the KNCHR and other stakeholders, specifically the legislature and executive on how to address the challenges curtailing the performance of the KNCHR in general and particularly ways in which the Commission could go about in promoting, protecting and monitoring SERs.
- Full Text:
- Date Issued: 2018
The efficacy of multi-track diplomacy in resolving intrastate and internationalised conflicts in Africa: the case of the 2007/2008 post-election violence in Kenya
- Authors: Natolooka, Kepha
- Date: 2018
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63546 , vital:28434
- Description: Expected release date-April 2019
- Full Text:
- Date Issued: 2018
- Authors: Natolooka, Kepha
- Date: 2018
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63546 , vital:28434
- Description: Expected release date-April 2019
- Full Text:
- Date Issued: 2018
Medicine use in swallowing-impaired patients: Pharmacists’ knowledge, practice and information needs
- Authors: Masilamoney, Mehrusha
- Date: 2018
- Subjects: Deglutition disorders , Drugs -- Administration , Oral medication -- Administration , Pharmacists -- Practice , South African Pharmacy Council
- Language: English
- Type: text , Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10962/61940 , vital:28086
- Description: Dysphagia, or swallowing impairment, is a growing problem that affects 13.5% of the general population. The ability to swallow is essential for patients taking oral medicines, so this presents a challenge for swallowing-impaired (SI) patients as tablets and capsules will usually require modification prior to ingestion. Pharmacists should play a central role in advising SI patients about their medicine use, as well as problems that may impact on safety, adherence and therapeutic outcome. However, little is known about pharmacists’ level of knowledge, their practice and their information needs when dealing with SI patients and their use of medicines. The aim of this study was to investigate pharmacist knowledge, practice and information needs relating to the support of SI patients and their medicine-related needs. The study design included both quantitative and qualitative methods. A quantitative questionnaire was developed to collect data on the knowledge, practice and information needs of pharmacists and was piloted in 10 pharmacists, which resulted in minor modifications. The questionnaire was converted to a web-based survey and emailed to all pharmacists registered with the South African Pharmacy Council. Two knowledge scores were generated by summating correct responses: knowledge of dysphagia (KOD) and knowledge of medicine use (KOMU) in SI patients. Correlation analysis was used to investigate the strength of the relationship between specific variables with KOD and KOMU using the Pearson correlation coefficient. Qualitative semi-structured interviews were conducted with pharmacists from community, hospital and primary healthcare clinics in both a small town and a major metropole. The aim was to gain deeper understanding of issues arising from the survey, and to explore preferences for topic-specific information materials. All interviews were audio-recorded and transcribed verbatim. Thematic analysis was used to analyse the data. A total of 439 pharmacists responded to the survey, with 67% being females.The mean KOD score out of a maximum score of 10 was 6.1 ± 1.8. KOD was inadequate (<5) in just over one-third (37.8%) of pharmacists. The mean KOMU score achieved (maximum score 17) was 9.4 ± 2.0, with inadequate knowledge (<10) being established in just over two-thirds of pharmacists (70.8%). Age, length of registration as a pharmacist, and years of practice in a setting with direct patient interaction were significantly but weakly correlated with KOMU, whereas KOD showed no significant association with these variables. Qualification significantly influenced both KOD and KOMU; the highest group with adequate knowledge had either a Masters or a PharmD degree. Fewer than half the pharmacists (44%) never ask patients about their swallowing ability, and most (86%) reported no knowledge of locally available viscosity enhancers. Almost all pharmacists were interested in receiving information materials on assisting SI patients with their medicine use. Three major themes emerged from the semi-structured interviews. Pharmacists recognised their knowledge deficit and felt that lack of both undergraduate training and formal training during practice, as well as limited exposure to SI patients, were contributing factors. Barriers to their practice with SI patients included lack of time, lack of institutional support and lack of easily accessible references on the pharmacists’ role in supporting medicine use in SI patients. Lastly, most pharmacists were not prepared to take ownership of medicine-related problems in SI patients and had conflicting opinions of the pharmacists’ role, usually shifting the responsibility of medicine use in SI patients to nurses. This is the first study to investigate pharmacist knowledge of medicine use in SI patients. The findings indicate that pharmacists do not have the requisite knowledge when dealing with SI patients and their medicine-taking issues despite being the most highly trained healthcare professionals in this field. Lack of undergraduate training, in-house training and limited exposure to SI patients were reported to contribute to poor knowledge. Current practice revealed that there appears to be poor communication among different healthcare professionals, pharmacists were reluctant to work with and/or train nurses on appropriate medicine use in SI patients, and there appeared to be ambiguity surrounding the role of a pharmacist. This research identified that pharmacists regard this topic to be highly relevant to their everyday practice and are keen to receive more information and training relating to this area of study. Information materials were designed and will be made accessible to all pharmacists registered in South Africa.
- Full Text:
- Date Issued: 2018
Medicine use in swallowing-impaired patients: Pharmacists’ knowledge, practice and information needs
- Authors: Masilamoney, Mehrusha
- Date: 2018
- Subjects: Deglutition disorders , Drugs -- Administration , Oral medication -- Administration , Pharmacists -- Practice , South African Pharmacy Council
- Language: English
- Type: text , Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10962/61940 , vital:28086
- Description: Dysphagia, or swallowing impairment, is a growing problem that affects 13.5% of the general population. The ability to swallow is essential for patients taking oral medicines, so this presents a challenge for swallowing-impaired (SI) patients as tablets and capsules will usually require modification prior to ingestion. Pharmacists should play a central role in advising SI patients about their medicine use, as well as problems that may impact on safety, adherence and therapeutic outcome. However, little is known about pharmacists’ level of knowledge, their practice and their information needs when dealing with SI patients and their use of medicines. The aim of this study was to investigate pharmacist knowledge, practice and information needs relating to the support of SI patients and their medicine-related needs. The study design included both quantitative and qualitative methods. A quantitative questionnaire was developed to collect data on the knowledge, practice and information needs of pharmacists and was piloted in 10 pharmacists, which resulted in minor modifications. The questionnaire was converted to a web-based survey and emailed to all pharmacists registered with the South African Pharmacy Council. Two knowledge scores were generated by summating correct responses: knowledge of dysphagia (KOD) and knowledge of medicine use (KOMU) in SI patients. Correlation analysis was used to investigate the strength of the relationship between specific variables with KOD and KOMU using the Pearson correlation coefficient. Qualitative semi-structured interviews were conducted with pharmacists from community, hospital and primary healthcare clinics in both a small town and a major metropole. The aim was to gain deeper understanding of issues arising from the survey, and to explore preferences for topic-specific information materials. All interviews were audio-recorded and transcribed verbatim. Thematic analysis was used to analyse the data. A total of 439 pharmacists responded to the survey, with 67% being females.The mean KOD score out of a maximum score of 10 was 6.1 ± 1.8. KOD was inadequate (<5) in just over one-third (37.8%) of pharmacists. The mean KOMU score achieved (maximum score 17) was 9.4 ± 2.0, with inadequate knowledge (<10) being established in just over two-thirds of pharmacists (70.8%). Age, length of registration as a pharmacist, and years of practice in a setting with direct patient interaction were significantly but weakly correlated with KOMU, whereas KOD showed no significant association with these variables. Qualification significantly influenced both KOD and KOMU; the highest group with adequate knowledge had either a Masters or a PharmD degree. Fewer than half the pharmacists (44%) never ask patients about their swallowing ability, and most (86%) reported no knowledge of locally available viscosity enhancers. Almost all pharmacists were interested in receiving information materials on assisting SI patients with their medicine use. Three major themes emerged from the semi-structured interviews. Pharmacists recognised their knowledge deficit and felt that lack of both undergraduate training and formal training during practice, as well as limited exposure to SI patients, were contributing factors. Barriers to their practice with SI patients included lack of time, lack of institutional support and lack of easily accessible references on the pharmacists’ role in supporting medicine use in SI patients. Lastly, most pharmacists were not prepared to take ownership of medicine-related problems in SI patients and had conflicting opinions of the pharmacists’ role, usually shifting the responsibility of medicine use in SI patients to nurses. This is the first study to investigate pharmacist knowledge of medicine use in SI patients. The findings indicate that pharmacists do not have the requisite knowledge when dealing with SI patients and their medicine-taking issues despite being the most highly trained healthcare professionals in this field. Lack of undergraduate training, in-house training and limited exposure to SI patients were reported to contribute to poor knowledge. Current practice revealed that there appears to be poor communication among different healthcare professionals, pharmacists were reluctant to work with and/or train nurses on appropriate medicine use in SI patients, and there appeared to be ambiguity surrounding the role of a pharmacist. This research identified that pharmacists regard this topic to be highly relevant to their everyday practice and are keen to receive more information and training relating to this area of study. Information materials were designed and will be made accessible to all pharmacists registered in South Africa.
- Full Text:
- Date Issued: 2018
The impact of South African business on employment relations in Mozambique: a case study of Banco Austral, a subsidiary of ABSA
- Mtyingizana, Beata Nontlahla
- Authors: Mtyingizana, Beata Nontlahla
- Date: 2018
- Subjects: Industrial sociology -- Mozambique , Industrial relations -- Mozambique , Industrial relations -- Cross-cultural studies , Industrial sociology -- Political aspects , Personnel management -- Mozambique , Personnel management -- Political aspects , ABSA Bank , Banco Austral
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60267 , vital:27760
- Description: This thesis examines the impact of South African business on employment relations in Mozambique. The study specifically focuses on a case study of Banco Austral, a subsidiary of the Amalgamated Banks of South Africa (ABSA), to provide a richer and deeper analysis of employment practices. The study examines the extent to which the expansion of South African businesses in Mozambique has influenced the development of employment relations there. It examines whether the employment relations that exist at Banco Austral are a direct result of the influence of its parent firm, ABSA. It assesses whether the prevailing managerial practices at Banco Austral are distinctly local and a product of Mozambican history or whether the employer and employee relations that exist in that bank are an outcome of some form of hybridised host country and parent firm practices. The study is contextualised within the period of transition that both countries underwent. In the South African context, the end of apartheid promised political stability, democracy and racial harmony. It facilitated South Africa’s reinsertion into the international economy and enabled its investors to embark on large-scale penetration of potential markets. It also opened up opportunities for investors to take advantage of the new global order in the rest of Africa and particularly in Mozambique. Similarly, in Mozambique, the transition to capitalism and to liberal democracy in 1994 marked a clear shift of policy from an authoritarian one-party state characterised by a planned socialist economy. In embracing a liberal market economy, Mozambique facilitated a transference of ownership of major state assets to the private sector and opened itself to the private sector’s market-driven business practices and managerial cultures. The study found that the impact of these historical developments on the evolution of employment relations at Banco Austral has been contradictory. ABSA’s ownership of Banco Austral opened as many opportunities as it closed them. On the one hand, it marked an opportunity for the growth and development of the ABSA brand across Mozambique. It presented opportunities for the integration of the Mozambican workforce into the larger ABSA Group and thereby enabling the transference of innovative managerial practices, world class human resources management techniques and advanced banking technology from ABSA and into Banco Austral. ABSA’s acquisition of Banco Austral also opened prospects for the development of skills for the workforce, enhanced the chances of job mobility as well as opened opportunities for Banco Austral workers to take advantage of the newly created jobs. On the other hand, however, the managerial practices and the human resource management techniques transferred from ABSA was reported by Banco Austral workers to be far from innovative. Instead, they resembled traits of past practices characteristic of Mozambique’s colonial workplace regime and South Africa’s apartheid workplace regime. This tilted the balance of power in favour of managers who arbitrarily exercised their authority in ways similar to that of the Portuguese managers in colonial Mozambique and that of the production councils during the post-independence socialist period. Access to senior positions and training opportunities continued to be allocated along national and racial lines. Many Mozambican workers were said to be ABSA-unfit and could not be trained in critical areas to improve their relevance in the larger ABSA Group. Despite these findings however, this study shows that Banco Austral workers were not helpless actors in the employment relationship. Rather, they remained agents of change in their own right, endowed with varying degrees of power, which they used to minimise the impact of arbitrary management practices and influence the direction of the employment relationship to advance their interests. The argument of this thesis is advanced by locating the study within Pierre Bourdieu’s theoretical triad of capital, field and habitus. Bourdieu’s theoretical triad is used as important analytical tools for understanding how ABSA-specific practices evolved and continued to shape the conduct and thoughts of its managers and workers alike. This study also makes special contribution to Marxism by revisiting Marx’s conception of the labour process to expose a number of analytical dilemmas when Marx is applied to bank labour.
- Full Text:
- Date Issued: 2018
- Authors: Mtyingizana, Beata Nontlahla
- Date: 2018
- Subjects: Industrial sociology -- Mozambique , Industrial relations -- Mozambique , Industrial relations -- Cross-cultural studies , Industrial sociology -- Political aspects , Personnel management -- Mozambique , Personnel management -- Political aspects , ABSA Bank , Banco Austral
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60267 , vital:27760
- Description: This thesis examines the impact of South African business on employment relations in Mozambique. The study specifically focuses on a case study of Banco Austral, a subsidiary of the Amalgamated Banks of South Africa (ABSA), to provide a richer and deeper analysis of employment practices. The study examines the extent to which the expansion of South African businesses in Mozambique has influenced the development of employment relations there. It examines whether the employment relations that exist at Banco Austral are a direct result of the influence of its parent firm, ABSA. It assesses whether the prevailing managerial practices at Banco Austral are distinctly local and a product of Mozambican history or whether the employer and employee relations that exist in that bank are an outcome of some form of hybridised host country and parent firm practices. The study is contextualised within the period of transition that both countries underwent. In the South African context, the end of apartheid promised political stability, democracy and racial harmony. It facilitated South Africa’s reinsertion into the international economy and enabled its investors to embark on large-scale penetration of potential markets. It also opened up opportunities for investors to take advantage of the new global order in the rest of Africa and particularly in Mozambique. Similarly, in Mozambique, the transition to capitalism and to liberal democracy in 1994 marked a clear shift of policy from an authoritarian one-party state characterised by a planned socialist economy. In embracing a liberal market economy, Mozambique facilitated a transference of ownership of major state assets to the private sector and opened itself to the private sector’s market-driven business practices and managerial cultures. The study found that the impact of these historical developments on the evolution of employment relations at Banco Austral has been contradictory. ABSA’s ownership of Banco Austral opened as many opportunities as it closed them. On the one hand, it marked an opportunity for the growth and development of the ABSA brand across Mozambique. It presented opportunities for the integration of the Mozambican workforce into the larger ABSA Group and thereby enabling the transference of innovative managerial practices, world class human resources management techniques and advanced banking technology from ABSA and into Banco Austral. ABSA’s acquisition of Banco Austral also opened prospects for the development of skills for the workforce, enhanced the chances of job mobility as well as opened opportunities for Banco Austral workers to take advantage of the newly created jobs. On the other hand, however, the managerial practices and the human resource management techniques transferred from ABSA was reported by Banco Austral workers to be far from innovative. Instead, they resembled traits of past practices characteristic of Mozambique’s colonial workplace regime and South Africa’s apartheid workplace regime. This tilted the balance of power in favour of managers who arbitrarily exercised their authority in ways similar to that of the Portuguese managers in colonial Mozambique and that of the production councils during the post-independence socialist period. Access to senior positions and training opportunities continued to be allocated along national and racial lines. Many Mozambican workers were said to be ABSA-unfit and could not be trained in critical areas to improve their relevance in the larger ABSA Group. Despite these findings however, this study shows that Banco Austral workers were not helpless actors in the employment relationship. Rather, they remained agents of change in their own right, endowed with varying degrees of power, which they used to minimise the impact of arbitrary management practices and influence the direction of the employment relationship to advance their interests. The argument of this thesis is advanced by locating the study within Pierre Bourdieu’s theoretical triad of capital, field and habitus. Bourdieu’s theoretical triad is used as important analytical tools for understanding how ABSA-specific practices evolved and continued to shape the conduct and thoughts of its managers and workers alike. This study also makes special contribution to Marxism by revisiting Marx’s conception of the labour process to expose a number of analytical dilemmas when Marx is applied to bank labour.
- Full Text:
- Date Issued: 2018
Bringing us back
- Authors: Dhliwayo, Mercy
- Date: 2018
- Subjects: South African fiction (English) -- 21st century , Short stories, South African (English) -- 21st century
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/63077 , vital:28361
- Description: My thesis takes the form of a collection of short stories set mostly in Zimbabwe and South Africa under the current political, social and economic climate. The themes I explore include forced migrations, identity, family disintegration and destitution. I use non-linear narration inspired by my reading of Dambudzo Marechera and Lidia Yuknavitch’s use of photographic imagery, in Black Sunlight and The Small Backs of Children respectively, to heighten my thematic concerns. The poetry in their language also serves as a source of inspiration, as does the graphic imagery used by Ayi Kwei Armah. In addition, I draw on the fragmented form used by Deepak Unnikrishnan to explore migration in his collection, Temporary People and Miljenko Jergovic’s investigation of violence and displacement in Sarajevo Marlboro.
- Full Text:
- Date Issued: 2018
- Authors: Dhliwayo, Mercy
- Date: 2018
- Subjects: South African fiction (English) -- 21st century , Short stories, South African (English) -- 21st century
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/63077 , vital:28361
- Description: My thesis takes the form of a collection of short stories set mostly in Zimbabwe and South Africa under the current political, social and economic climate. The themes I explore include forced migrations, identity, family disintegration and destitution. I use non-linear narration inspired by my reading of Dambudzo Marechera and Lidia Yuknavitch’s use of photographic imagery, in Black Sunlight and The Small Backs of Children respectively, to heighten my thematic concerns. The poetry in their language also serves as a source of inspiration, as does the graphic imagery used by Ayi Kwei Armah. In addition, I draw on the fragmented form used by Deepak Unnikrishnan to explore migration in his collection, Temporary People and Miljenko Jergovic’s investigation of violence and displacement in Sarajevo Marlboro.
- Full Text:
- Date Issued: 2018
Bioprospecting for amylases, cellulases and xylanases from ericoid associated fungi, their production and characterisation for the bio-economy
- Authors: Adeoyo, Olusegun Richard
- Date: 2018
- Subjects: Mycorrhizal fungi , Hydrolases , Ericaceae South Africa , Ericaceae Molecular aspects
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/64327 , vital:28533
- Description: South Africa is one of the most productive areas for ericaceous plants with about 850 identified species in the Cape Floral Region. The Albany Centre of Endemism where all fungi used in this study were isolated from, falls within this region. Ericaceous plants interact with some fungi via an association called the ericoid mycorrhizal (ERM) association. All fungi used in this study were isolated from roots of six ericaceous plants; Erica cerinthoides, Erica demissa, Erica chamissonis, Erica glumiflora, Erica caffra and Erica nemorosa. Fungal enzymes are known to play a significant role in the food, brewing, detergent, pharmaceutical and biofuel industries. The enzyme industry is among the major sectors of the world, and additional novel sources are being explored from time to time. This study focussed on amylases (amyloglucosidase, AMG), cellulases (endoglucanase) and xylanases (endo-1,4-P-xylanase) production from ERM fungal isolates. Out of the fifty-one (51), fungal isolates screened, ChemRU330 (Leohumicola sp.), EdRU083 and EdRU002 were among the fungi that had the highest activities of all the enzymes. They were tested for the ability to produce amylases and cellulases under different pH and nutritional conditions that included: carbon sources, nitrogen sources and metal ions, at an optimum temperature of 28°C in a modified Melin-Norkrans (MMN) liquid medium. Cellulase specific activity of 3.99, 2.18 and 4.31 (U/mg protein) for isolates EdRU083, EdRU002 and ChemRU330, respectively, was produced at an optimal pH of 5.0. For amylase, ChemRU330 had the highest specific activity of 1.11 U/mg protein while EdRU083 and EdRU02 had a specific activity of 0.80 and 0.92 U/mg protein, respectively, at the same pH with corresponding biomass yield of 113, 125 and 97 mg/50 ml, respectively. Increased enzyme activities and improved mycelial biomass production were obtained in the presence of supplements such as potassium, sodium, glucose, maltose, cellobiose, tryptone and peptone, while NaFe-EDTA and cobalt inhibited enzyme activity. ChemRU330 was selected to determine the consistency and amount of amylase, cellulase and xylanase formed after several in vitro subculturing events. AMG and endo-1,4-P-xylanase were found to have the most consistent production throughout the study period. The AMG was stable at 45oC (pH 5.0), retaining approximately 65% activity over a period of 24 h. The molecular mass of AMG and endo-1,4-P-xylanase were estimated to be 101 kDa and 72 kDa, respectively. The Km and kcat were 0.38 mg/ml and 70 s-1, respectively, using soluble starch (AMG). For endo-1,4-P-xylanase, the Km and Vmax were 0.93 mg/ml and 8.54 U/ml, respectively, using beechwood xylan (endo-1,4-P-xylanase) as substrate. Additionally, crude extracts of five root endophytes with unique morphological characteristics were screened for antibacterial properties and was followed by determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). L. incrustata (ChemRU330) and Chaetomium sp. extracts exhibited varying degrees of inhibition against two Gram-positive and Gram-negative bacteria. The crude extract of L. incrustata was the most effective which was found to inhibit Staphylococcus aureus (MIC: 1 mg/ml), Bacillus subtilis (MIC: 2 mg/ml) and Proteus vulgaris (MIC: 16 mg/ml). The L. incrustata displayed potential for antibacterial production and could be considered as an additional source of new antimicrobial agents in drug and food preservation. Also, the three isolates used for enzyme production were identified to genus and species levels, i.e., Leohumicola incrustata (ChemRU330), Leohumicola sp. (EdRU083) and Oidiodendron sp. (EdRU002) using both ITS and Cox1 DNA regions. The molecular analysis results indicated that these ERM mycorrhizal fungi were similar to those successfully described by some researchers in South Africa and Australia. Therefore, this study opens new opportunities for exploring ERM fungal biomolecules for the bio-economy. The promising physicochemical properties, starch and xylan hydrolysis end- products, and being non-pathogenic make AMG and endo-1,4-P-xylanase potential candidates for future applications as additives in the food industry for the production of glucose, glucose syrups, high-fructose corn syrups, and as well as the production of bioethanol. Finally, the findings of this study revealed that it is possible to produce hydrolytic enzymes from ERM fungi in vitro using chemically defined media. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
- Authors: Adeoyo, Olusegun Richard
- Date: 2018
- Subjects: Mycorrhizal fungi , Hydrolases , Ericaceae South Africa , Ericaceae Molecular aspects
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/64327 , vital:28533
- Description: South Africa is one of the most productive areas for ericaceous plants with about 850 identified species in the Cape Floral Region. The Albany Centre of Endemism where all fungi used in this study were isolated from, falls within this region. Ericaceous plants interact with some fungi via an association called the ericoid mycorrhizal (ERM) association. All fungi used in this study were isolated from roots of six ericaceous plants; Erica cerinthoides, Erica demissa, Erica chamissonis, Erica glumiflora, Erica caffra and Erica nemorosa. Fungal enzymes are known to play a significant role in the food, brewing, detergent, pharmaceutical and biofuel industries. The enzyme industry is among the major sectors of the world, and additional novel sources are being explored from time to time. This study focussed on amylases (amyloglucosidase, AMG), cellulases (endoglucanase) and xylanases (endo-1,4-P-xylanase) production from ERM fungal isolates. Out of the fifty-one (51), fungal isolates screened, ChemRU330 (Leohumicola sp.), EdRU083 and EdRU002 were among the fungi that had the highest activities of all the enzymes. They were tested for the ability to produce amylases and cellulases under different pH and nutritional conditions that included: carbon sources, nitrogen sources and metal ions, at an optimum temperature of 28°C in a modified Melin-Norkrans (MMN) liquid medium. Cellulase specific activity of 3.99, 2.18 and 4.31 (U/mg protein) for isolates EdRU083, EdRU002 and ChemRU330, respectively, was produced at an optimal pH of 5.0. For amylase, ChemRU330 had the highest specific activity of 1.11 U/mg protein while EdRU083 and EdRU02 had a specific activity of 0.80 and 0.92 U/mg protein, respectively, at the same pH with corresponding biomass yield of 113, 125 and 97 mg/50 ml, respectively. Increased enzyme activities and improved mycelial biomass production were obtained in the presence of supplements such as potassium, sodium, glucose, maltose, cellobiose, tryptone and peptone, while NaFe-EDTA and cobalt inhibited enzyme activity. ChemRU330 was selected to determine the consistency and amount of amylase, cellulase and xylanase formed after several in vitro subculturing events. AMG and endo-1,4-P-xylanase were found to have the most consistent production throughout the study period. The AMG was stable at 45oC (pH 5.0), retaining approximately 65% activity over a period of 24 h. The molecular mass of AMG and endo-1,4-P-xylanase were estimated to be 101 kDa and 72 kDa, respectively. The Km and kcat were 0.38 mg/ml and 70 s-1, respectively, using soluble starch (AMG). For endo-1,4-P-xylanase, the Km and Vmax were 0.93 mg/ml and 8.54 U/ml, respectively, using beechwood xylan (endo-1,4-P-xylanase) as substrate. Additionally, crude extracts of five root endophytes with unique morphological characteristics were screened for antibacterial properties and was followed by determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). L. incrustata (ChemRU330) and Chaetomium sp. extracts exhibited varying degrees of inhibition against two Gram-positive and Gram-negative bacteria. The crude extract of L. incrustata was the most effective which was found to inhibit Staphylococcus aureus (MIC: 1 mg/ml), Bacillus subtilis (MIC: 2 mg/ml) and Proteus vulgaris (MIC: 16 mg/ml). The L. incrustata displayed potential for antibacterial production and could be considered as an additional source of new antimicrobial agents in drug and food preservation. Also, the three isolates used for enzyme production were identified to genus and species levels, i.e., Leohumicola incrustata (ChemRU330), Leohumicola sp. (EdRU083) and Oidiodendron sp. (EdRU002) using both ITS and Cox1 DNA regions. The molecular analysis results indicated that these ERM mycorrhizal fungi were similar to those successfully described by some researchers in South Africa and Australia. Therefore, this study opens new opportunities for exploring ERM fungal biomolecules for the bio-economy. The promising physicochemical properties, starch and xylan hydrolysis end- products, and being non-pathogenic make AMG and endo-1,4-P-xylanase potential candidates for future applications as additives in the food industry for the production of glucose, glucose syrups, high-fructose corn syrups, and as well as the production of bioethanol. Finally, the findings of this study revealed that it is possible to produce hydrolytic enzymes from ERM fungi in vitro using chemically defined media. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
Human capital and other stories
- Authors: Dludlu, John
- Date: 2018
- Subjects: Short stories, South African (English) -- 21st century , South African fiction (English) -- 21st century
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/63121 , vital:28365
- Description: My collection of short stories is set mostly in Gauteng and revolves around mainly the lives of the urban, black elite almost three decades after the first non‐racial elections in South Africa. It captures emerging trends and fault lines and enquires into whether South Africa can continue on a different path from that of the rest of the continent. Themes covered in the collection, which still espouses idealism, include the acquisition of power, status and money, the use and abuse of these, as well as the psychosocial effects of money on this group. My writing is inspired by the courageous, inventive and introspective writings of the Drum generation of writers William Bloke Modisane, Nat Nakasa and Can Themba, as well as the use of language and the experimental form of writing as embodied in the work of Lidia Yuknavitch to deal with similarly pressing social issues of the day.
- Full Text:
- Date Issued: 2018
- Authors: Dludlu, John
- Date: 2018
- Subjects: Short stories, South African (English) -- 21st century , South African fiction (English) -- 21st century
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/63121 , vital:28365
- Description: My collection of short stories is set mostly in Gauteng and revolves around mainly the lives of the urban, black elite almost three decades after the first non‐racial elections in South Africa. It captures emerging trends and fault lines and enquires into whether South Africa can continue on a different path from that of the rest of the continent. Themes covered in the collection, which still espouses idealism, include the acquisition of power, status and money, the use and abuse of these, as well as the psychosocial effects of money on this group. My writing is inspired by the courageous, inventive and introspective writings of the Drum generation of writers William Bloke Modisane, Nat Nakasa and Can Themba, as well as the use of language and the experimental form of writing as embodied in the work of Lidia Yuknavitch to deal with similarly pressing social issues of the day.
- Full Text:
- Date Issued: 2018
Two-tissue stable isotope analysis to elucidate isotopic incorporation and trophic niche patterns for chubbyhead barb Enteromius anoplus
- Authors: Kambikambi, Manda Juliet
- Date: 2018
- Subjects: Food chains (Ecology) , Barbus -- South Africa -- Great Fish River Estuary , Stable isotopes , Freshwater fishes -- Feeding and feeds , Freshwater fishes -- Food , Fins (Anatomy) , Akaike Information Criterion , Freshwater fishes -- Conservation , Chubbyhead barb Enteromius anoplus
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/61906 , vital:28082
- Description: Knowledge of trophic ecology underpins conservation and management of threatened species. Stable isotope analysis has been widely used as a more objective approach for elucidating the trophic positions of freshwater fishes. Until recently, stable isotope analysis for trophic ecology studies in freshwater fishes largely utilised white muscle tissue. This sampling approach, however, involves either euthanasia or muscle biopsy procedures that may be inappropriate for small-sized and endangered fishes. These concerns raised the need to explore and validate the utility of non-lethal alternatives such as fin clips, mucus and scales. The present study investigated the use of caudal fin tissue as a potential non-lethal alternative to muscle tissue for trophic studies on the chubbyhead barb Enteromius anoplus. The chubbyhead barb was selected as a model taxon for the present study because it is closely related or comparable in body size to a number of highly threatened small-bodied minnows in southern Africa. The chubbyhead barb was also considered an ideal species for this study because it is widespread, abundant and classified as Least Concern on the IUCN list of threatened species. The study used a two-pronged approach based on laboratory and field experiments. A laboratory experiment was conducted to quantify isotopic turnover rates and diet-tissue discrimination factors (DTDFs/A) for both muscle and fin tissues. This involved feeding chubbyhead barb two diets with distinct carbon (δ13C) and nitrogen (δ15N) values, and monitoring the temporal isotopic incorporation patterns into the two tissues. These patterns were assessed by applying least squares non-linear one- and two-compartment isotopic kinetics models. Model comparisons, based on Akaike information criterion (AIC), revealed that one- compartment models described isotopic incorporation patterns better than two-compartment models for both muscle and fin tissues. For δ13C, relatively short and comparable turnover rates were observed for muscle and fin tissues, which suggests that fin tissue could potentially provide similar inference as muscle tissue when assessing short term dietary patterns for chubbyhead barb. In contrast to δ13C, turnover rates for δ15N between muscle and fin tissue were different for both diets. Specifically, stable isotope incorporation turnover rate was faster in muscle tissue for animals that were fed on isotopically enriched diets compared to fin tissue. Conversely, stable isotope incorporation into fin tissue was faster in animals fed on isotopically depleted diets compared to muscle tissue. This suggests that knowledge of animal diet is critical when inferring fin tissue δ15N turnover rates, particularly when extrapolating both short and long term dietary patterns. Diet-tissue discrimination factors were influenced by diet type, with the fish fed on isotopically enriched diet having lower DTDFs than animals fed on isotopically depleted diets. This variation may be explained by the protein quality hypothesis, which suggests that the DTDFs of consumers will decrease as protein quality increases. When A13C and A15N values were averaged across diets in muscle and fin tissue, the values were 0.74‰ and 0.64‰, respectively, for A13C, and 5.53‰ and 5.83 ‰, respectively, for A15N. This appeared to be consistent with studies on other taxa for A13C (0-1 ‰), but for A15N (3-5 ‰) the results of this study were higher than those reported for other taxa. These results suggest that investigating appropriate DTDFs for both muscle and fin tissues is important in trophic ecology studies of these minnows. A field-based study was conducted to investigate temporal dynamics in food web patterns for chubbyhead barb in the wild within the headwaters of the Koonap River, a tributary of the Great Fish River, in the Eastern Cape, South Africa. This was achieved by collecting and comparing stable isotope data for chubbyhead barb and its potential food sources on a seasonal scale. There was a discernible difference in both the composition of carbon and nitrogen isotope values for basal food sources and macroinvertebrate communities, which suggests that this headwater stream was subject to temporal changes in food web dynamics. For chubbyhead barb, comparison of its isotopic niche sizes on a temporal scale based on both muscle and fin tissue showed differences across seasons. Furthermore, isotopic niche sizes inferred from fin tissue were larger than those inferred from muscle tissue during winter and spring, whereas during summer and autumn the isotopic niche sizes inferred from muscle and fin tissue were generally comparable. This suggests the likely influence of different metabolic and physiological processes that these two tissues undergo on a temporal scale. Therefore, difference in tissue type, and their associated metabolic pathways should be considered when using fin tissue as a substitute for muscle tissue on broad temporal scales. The results from this study indicated that caudal fin tissue has the potential to be a substitute for muscle in trophic studies of chubbyhead barb Enteromius anoplus, as well as other related small bodied endangered minnow species from South Africa.
- Full Text:
- Date Issued: 2018
- Authors: Kambikambi, Manda Juliet
- Date: 2018
- Subjects: Food chains (Ecology) , Barbus -- South Africa -- Great Fish River Estuary , Stable isotopes , Freshwater fishes -- Feeding and feeds , Freshwater fishes -- Food , Fins (Anatomy) , Akaike Information Criterion , Freshwater fishes -- Conservation , Chubbyhead barb Enteromius anoplus
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/61906 , vital:28082
- Description: Knowledge of trophic ecology underpins conservation and management of threatened species. Stable isotope analysis has been widely used as a more objective approach for elucidating the trophic positions of freshwater fishes. Until recently, stable isotope analysis for trophic ecology studies in freshwater fishes largely utilised white muscle tissue. This sampling approach, however, involves either euthanasia or muscle biopsy procedures that may be inappropriate for small-sized and endangered fishes. These concerns raised the need to explore and validate the utility of non-lethal alternatives such as fin clips, mucus and scales. The present study investigated the use of caudal fin tissue as a potential non-lethal alternative to muscle tissue for trophic studies on the chubbyhead barb Enteromius anoplus. The chubbyhead barb was selected as a model taxon for the present study because it is closely related or comparable in body size to a number of highly threatened small-bodied minnows in southern Africa. The chubbyhead barb was also considered an ideal species for this study because it is widespread, abundant and classified as Least Concern on the IUCN list of threatened species. The study used a two-pronged approach based on laboratory and field experiments. A laboratory experiment was conducted to quantify isotopic turnover rates and diet-tissue discrimination factors (DTDFs/A) for both muscle and fin tissues. This involved feeding chubbyhead barb two diets with distinct carbon (δ13C) and nitrogen (δ15N) values, and monitoring the temporal isotopic incorporation patterns into the two tissues. These patterns were assessed by applying least squares non-linear one- and two-compartment isotopic kinetics models. Model comparisons, based on Akaike information criterion (AIC), revealed that one- compartment models described isotopic incorporation patterns better than two-compartment models for both muscle and fin tissues. For δ13C, relatively short and comparable turnover rates were observed for muscle and fin tissues, which suggests that fin tissue could potentially provide similar inference as muscle tissue when assessing short term dietary patterns for chubbyhead barb. In contrast to δ13C, turnover rates for δ15N between muscle and fin tissue were different for both diets. Specifically, stable isotope incorporation turnover rate was faster in muscle tissue for animals that were fed on isotopically enriched diets compared to fin tissue. Conversely, stable isotope incorporation into fin tissue was faster in animals fed on isotopically depleted diets compared to muscle tissue. This suggests that knowledge of animal diet is critical when inferring fin tissue δ15N turnover rates, particularly when extrapolating both short and long term dietary patterns. Diet-tissue discrimination factors were influenced by diet type, with the fish fed on isotopically enriched diet having lower DTDFs than animals fed on isotopically depleted diets. This variation may be explained by the protein quality hypothesis, which suggests that the DTDFs of consumers will decrease as protein quality increases. When A13C and A15N values were averaged across diets in muscle and fin tissue, the values were 0.74‰ and 0.64‰, respectively, for A13C, and 5.53‰ and 5.83 ‰, respectively, for A15N. This appeared to be consistent with studies on other taxa for A13C (0-1 ‰), but for A15N (3-5 ‰) the results of this study were higher than those reported for other taxa. These results suggest that investigating appropriate DTDFs for both muscle and fin tissues is important in trophic ecology studies of these minnows. A field-based study was conducted to investigate temporal dynamics in food web patterns for chubbyhead barb in the wild within the headwaters of the Koonap River, a tributary of the Great Fish River, in the Eastern Cape, South Africa. This was achieved by collecting and comparing stable isotope data for chubbyhead barb and its potential food sources on a seasonal scale. There was a discernible difference in both the composition of carbon and nitrogen isotope values for basal food sources and macroinvertebrate communities, which suggests that this headwater stream was subject to temporal changes in food web dynamics. For chubbyhead barb, comparison of its isotopic niche sizes on a temporal scale based on both muscle and fin tissue showed differences across seasons. Furthermore, isotopic niche sizes inferred from fin tissue were larger than those inferred from muscle tissue during winter and spring, whereas during summer and autumn the isotopic niche sizes inferred from muscle and fin tissue were generally comparable. This suggests the likely influence of different metabolic and physiological processes that these two tissues undergo on a temporal scale. Therefore, difference in tissue type, and their associated metabolic pathways should be considered when using fin tissue as a substitute for muscle tissue on broad temporal scales. The results from this study indicated that caudal fin tissue has the potential to be a substitute for muscle in trophic studies of chubbyhead barb Enteromius anoplus, as well as other related small bodied endangered minnow species from South Africa.
- Full Text:
- Date Issued: 2018
Understanding and quantifying channel transmission loss processes in the Limpopo River Basin
- Authors: Mvandaba, Vuyelwa
- Date: 2018
- Subjects: Water-supply -- Management , Water-supply -- Limpopo River Watershed , Alluvium -- Limpopo River Watershed , Streamflow -- Limpopo River Watershed
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63797 , vital:28490
- Description: Water availability is one of the major societal issues facing the world. The ability to understand and quantify the impact of key hydrological processes on the availability of water resources is therefore integral to ensuring equitable and sustainable resource management. A review of previous hydrological studies conducted in the Limpopo River Basin has revealed a gap in the understanding of surface water-groundwater interactions, particularly channel transmission loss processes. These earlier studies, focused largely on the Limpopo River’s main stem, have attributed the existence of these streamflow losses to the presence of significant alluvial aquifers and indicated that the losses account for about 30 percent (or 1000 Mm3 a-1) of the basin’s water balance. The work conducted in this dissertation reports on the delineation of alluvial aquifers across three sub-basins of the Limpopo River Basin namely, the Mokolo (South Africa), Motloutse (Botswana) and Mzingwane (Zimbabwe) sub-basins and the estimation of potential channel transmission losses based on the alluvial aquifer properties. Additionally, an assessment of the different approaches that can be applied to simulate these channel transmission losses in the Pitman Model is presented. To delineate alluvial aquifers, general land cover classes including alluvial aquifers were produced from Landsat-8 imagery through image classification. The areal extent of the delineated alluvial aquifers was calculated using ArcMap 10.3. To quantify channel transmission losses and determine the effects on regional water resources, three approaches using the Pitman model were applied. The three approaches include an explicit transmission loss function, the use of a wetland function to represent channel-floodplain storage exchanges and the use of a ‘dummy’ reservoir to represent floodplain storage and evapotranspiration losses. Results indicate that all three approaches were able to simulate channel transmission losses, although with differing magnitudes. Observed monthly flow data were used to as a means of validating loss simulations however for each sub-basin, medium and low flows were over-simulated which accounts for water uses that were inefficiently represented due to lack of data. Knowledge of the structure of the transmission loss function dictates that it is better at representing the dynamics of channel transmission losses, as it takes into account the contribution of losses to groundwater recharge whereas the other two functions simply store water and release it back to the channel. Overall, the hydrological modelling results demonstrate the potential of each approach in reproducing the dynamics of channel transmission losses between channel and alluvial aquifer within an existing sub-basin scale hydrological model. It is believed that better quantification of losses and more efficient qualitative determination of the function which best represents transmission losses, can be attained with more reliable observed data. In conclusion, a study of this nature can be beneficial to water resource estimation programmes as it highlights the uncertainties related with quantifying channel transmission loss processes in a semi-arid environment.
- Full Text:
- Date Issued: 2018
- Authors: Mvandaba, Vuyelwa
- Date: 2018
- Subjects: Water-supply -- Management , Water-supply -- Limpopo River Watershed , Alluvium -- Limpopo River Watershed , Streamflow -- Limpopo River Watershed
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63797 , vital:28490
- Description: Water availability is one of the major societal issues facing the world. The ability to understand and quantify the impact of key hydrological processes on the availability of water resources is therefore integral to ensuring equitable and sustainable resource management. A review of previous hydrological studies conducted in the Limpopo River Basin has revealed a gap in the understanding of surface water-groundwater interactions, particularly channel transmission loss processes. These earlier studies, focused largely on the Limpopo River’s main stem, have attributed the existence of these streamflow losses to the presence of significant alluvial aquifers and indicated that the losses account for about 30 percent (or 1000 Mm3 a-1) of the basin’s water balance. The work conducted in this dissertation reports on the delineation of alluvial aquifers across three sub-basins of the Limpopo River Basin namely, the Mokolo (South Africa), Motloutse (Botswana) and Mzingwane (Zimbabwe) sub-basins and the estimation of potential channel transmission losses based on the alluvial aquifer properties. Additionally, an assessment of the different approaches that can be applied to simulate these channel transmission losses in the Pitman Model is presented. To delineate alluvial aquifers, general land cover classes including alluvial aquifers were produced from Landsat-8 imagery through image classification. The areal extent of the delineated alluvial aquifers was calculated using ArcMap 10.3. To quantify channel transmission losses and determine the effects on regional water resources, three approaches using the Pitman model were applied. The three approaches include an explicit transmission loss function, the use of a wetland function to represent channel-floodplain storage exchanges and the use of a ‘dummy’ reservoir to represent floodplain storage and evapotranspiration losses. Results indicate that all three approaches were able to simulate channel transmission losses, although with differing magnitudes. Observed monthly flow data were used to as a means of validating loss simulations however for each sub-basin, medium and low flows were over-simulated which accounts for water uses that were inefficiently represented due to lack of data. Knowledge of the structure of the transmission loss function dictates that it is better at representing the dynamics of channel transmission losses, as it takes into account the contribution of losses to groundwater recharge whereas the other two functions simply store water and release it back to the channel. Overall, the hydrological modelling results demonstrate the potential of each approach in reproducing the dynamics of channel transmission losses between channel and alluvial aquifer within an existing sub-basin scale hydrological model. It is believed that better quantification of losses and more efficient qualitative determination of the function which best represents transmission losses, can be attained with more reliable observed data. In conclusion, a study of this nature can be beneficial to water resource estimation programmes as it highlights the uncertainties related with quantifying channel transmission loss processes in a semi-arid environment.
- Full Text:
- Date Issued: 2018
Naming in Germany in the 20th century: a sociological study of naming in times of social change, with a focus on statistical problems in empirical onomastic research
- Authors: Huschka, Denis
- Date: 2018
- Subjects: Onomastics , Names, Personal -- Germany , Names, German -- Etymology , Names, German -- Social aspects , German language -- Etymology -- Names , German Socio Economic Panel Study (SOEP)
- Language: German , English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63054 , vital:28359
- Description: In this thesis names are used as social indicators to observe social change in Germany in the 20th century. The German Socio Economic Panel Study (SOEP) offers the rare opportunity to analyse representative survey data of first names. The empirical results of the analyses in this thesis offer a comprehensive picture on how the naming reality in Germany looks like and how naming changed in a period of about 100 years. Names can serve as social indicators. It is demonstrated how chosen names mirror social change in the German society: Name choices have become less traditional and more individual. Over time names from other world regions and cultures have found their way into the German culture. There are more different names in use today than 100 years ago and the names have become more evenly distributed over the population. Today children are less likely to share their names with many of their peers. These are signs of an increasingly individualised, transnationalised modern behaviour of the people in contemporary Germany. Almost all of these developments started earlier and tend to be more pronounced for girl’s names. The secularisation of the German society - however - did not cause substantial changes in naming over time. Christian names still are used to the greatest extent, but – possibly – not because they are regarded as being of Christian origin. The analyses of the social-structural influences on naming touch on some effects of education and status. The analyses of differences in naming between the two German states during the time of division adds some evidence to the real-life experience that naming in the communist East Germany was much more oriented towards the free western hemisphere – a kind of silent protest. Obviously naming was a possibility to distance oneself from an un-loved regime. On a methodological level referring to onomastics, the so-called „Large Number of are Events-Zone (LNRE)“, a feature of the distribution of names that has mostly been handled inappropriately up to now, is discussed with respect to its effects on name statistics when using samples. An alternative approach is proposed for the appropriate handling of this feature. , In der vorliegenden Arbeit werden Vornamen als soziale Indikatoren benutzt, um gesellschaftlichen Wandel im 20. Jahrhundert zu beschreiben. Das Soziooekonomische Panel (SOEP) bietet die seltene Möglichkeit, Umfragedaten über die Vornamen der Deutschen repräsentativ auszuwerten. Die empirischen Ergebnisse der vorliegenden Arbeit bieten einen umfassenden Einblick in die deutsche Vornamensrealität und über die Entwicklungen der Namensvergabe in 100 Jahren. Namen sind soziale Indikatoren. Es wird aufgezeigt, wie die Namensgebung den sozialen Wandel in der deutschen Gesellschaft spiegelt: Namenwahlen weisen über die Zeit weniger traditionelle Bezüge auf, sie wurden individueller. Namen aus anderen Kulturen und Ländern fanden Eingang in die deutsche Kultur. Es werden mehr verschiedene Namen benutzt als vor 100 Jahren und die typischerweise hoch konzentrierten Verteilungen der Vornamen stellen sich über die Zeit etwas weniger konzentriert dar. Heute geborene Kinder teilen ihre Namen mit anteilig weniger anderen Kindern ihrer Kohorte. Dies sind Anzeichen für eine individualisierte, transnational orientierte moderne Gesellschaft. Fast alle dieser Entwicklungen sind für Mädchennamen früher und in deutlicherem Maße zu beobachten. Die Säkularisierung der deutschen Gesellschaft hat hingegen wenig Einfluss auf die Vornamenswahlen genommen. Nach wie vor werden vor allem christliche Namen vergeben, auch wenn der christliche Bezug unter Umständen nicht mehr der maßgebliche Grund für die Auswahl ist. Die Analyse der sozialstrukturellen Einflüsse auf Namenswahlen bestätigt einige Effekte von Bildung und Status der Mütter. Die Analyse der Unterschiede in der Namensgebung der beiden deutschen Staaten während der 40 jährigen Teilung zeigt, dass der lebensweltliche Eindruck einer zunehmenden West-Orientierung der Namenswahlen ostdeutscher Eltern nicht trügt. Offenbar waren westliche Namen eine Möglichkeit, sich vom ungeliebten Regime zu distanzieren. Auf einer statistisch-methodischen Ebene wird eine bislang in der empirischen Onomastik unrichtig gehandhabte Besonderheit von Vornamensverteilungen – die Large Number of Rare Events-Zone (LNRE) – diskutiert und Lösungsvorschläge für den statistisch korrekten Umgang mit dieser Besonderheit in Gruppenvergleichen auf der Basis von Stichproben vorgelegt.
- Full Text:
- Date Issued: 2018
- Authors: Huschka, Denis
- Date: 2018
- Subjects: Onomastics , Names, Personal -- Germany , Names, German -- Etymology , Names, German -- Social aspects , German language -- Etymology -- Names , German Socio Economic Panel Study (SOEP)
- Language: German , English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63054 , vital:28359
- Description: In this thesis names are used as social indicators to observe social change in Germany in the 20th century. The German Socio Economic Panel Study (SOEP) offers the rare opportunity to analyse representative survey data of first names. The empirical results of the analyses in this thesis offer a comprehensive picture on how the naming reality in Germany looks like and how naming changed in a period of about 100 years. Names can serve as social indicators. It is demonstrated how chosen names mirror social change in the German society: Name choices have become less traditional and more individual. Over time names from other world regions and cultures have found their way into the German culture. There are more different names in use today than 100 years ago and the names have become more evenly distributed over the population. Today children are less likely to share their names with many of their peers. These are signs of an increasingly individualised, transnationalised modern behaviour of the people in contemporary Germany. Almost all of these developments started earlier and tend to be more pronounced for girl’s names. The secularisation of the German society - however - did not cause substantial changes in naming over time. Christian names still are used to the greatest extent, but – possibly – not because they are regarded as being of Christian origin. The analyses of the social-structural influences on naming touch on some effects of education and status. The analyses of differences in naming between the two German states during the time of division adds some evidence to the real-life experience that naming in the communist East Germany was much more oriented towards the free western hemisphere – a kind of silent protest. Obviously naming was a possibility to distance oneself from an un-loved regime. On a methodological level referring to onomastics, the so-called „Large Number of are Events-Zone (LNRE)“, a feature of the distribution of names that has mostly been handled inappropriately up to now, is discussed with respect to its effects on name statistics when using samples. An alternative approach is proposed for the appropriate handling of this feature. , In der vorliegenden Arbeit werden Vornamen als soziale Indikatoren benutzt, um gesellschaftlichen Wandel im 20. Jahrhundert zu beschreiben. Das Soziooekonomische Panel (SOEP) bietet die seltene Möglichkeit, Umfragedaten über die Vornamen der Deutschen repräsentativ auszuwerten. Die empirischen Ergebnisse der vorliegenden Arbeit bieten einen umfassenden Einblick in die deutsche Vornamensrealität und über die Entwicklungen der Namensvergabe in 100 Jahren. Namen sind soziale Indikatoren. Es wird aufgezeigt, wie die Namensgebung den sozialen Wandel in der deutschen Gesellschaft spiegelt: Namenwahlen weisen über die Zeit weniger traditionelle Bezüge auf, sie wurden individueller. Namen aus anderen Kulturen und Ländern fanden Eingang in die deutsche Kultur. Es werden mehr verschiedene Namen benutzt als vor 100 Jahren und die typischerweise hoch konzentrierten Verteilungen der Vornamen stellen sich über die Zeit etwas weniger konzentriert dar. Heute geborene Kinder teilen ihre Namen mit anteilig weniger anderen Kindern ihrer Kohorte. Dies sind Anzeichen für eine individualisierte, transnational orientierte moderne Gesellschaft. Fast alle dieser Entwicklungen sind für Mädchennamen früher und in deutlicherem Maße zu beobachten. Die Säkularisierung der deutschen Gesellschaft hat hingegen wenig Einfluss auf die Vornamenswahlen genommen. Nach wie vor werden vor allem christliche Namen vergeben, auch wenn der christliche Bezug unter Umständen nicht mehr der maßgebliche Grund für die Auswahl ist. Die Analyse der sozialstrukturellen Einflüsse auf Namenswahlen bestätigt einige Effekte von Bildung und Status der Mütter. Die Analyse der Unterschiede in der Namensgebung der beiden deutschen Staaten während der 40 jährigen Teilung zeigt, dass der lebensweltliche Eindruck einer zunehmenden West-Orientierung der Namenswahlen ostdeutscher Eltern nicht trügt. Offenbar waren westliche Namen eine Möglichkeit, sich vom ungeliebten Regime zu distanzieren. Auf einer statistisch-methodischen Ebene wird eine bislang in der empirischen Onomastik unrichtig gehandhabte Besonderheit von Vornamensverteilungen – die Large Number of Rare Events-Zone (LNRE) – diskutiert und Lösungsvorschläge für den statistisch korrekten Umgang mit dieser Besonderheit in Gruppenvergleichen auf der Basis von Stichproben vorgelegt.
- Full Text:
- Date Issued: 2018
The current utility of oligonucleotide aptamers in targeting the MUC1 mucin tumour marker
- Authors: Flanagan, Shane Patrick
- Date: 2018
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62969 , vital:28348
- Description: Expected release date-April 2020
- Full Text:
- Date Issued: 2018
- Authors: Flanagan, Shane Patrick
- Date: 2018
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62969 , vital:28348
- Description: Expected release date-April 2020
- Full Text:
- Date Issued: 2018
Beginner teachers’ leadership development opportunities: an interventionist case study in a rural combined school in the Ohangwena region, Namibia
- Authors: Ndakolonkoshi, Klaudia
- Date: 2018
- Subjects: School management and organization -- Namibia , Teacher participation in administration -- Namibia , Educational leadership -- Namibia , Teacher effectiveness -- Namibia
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/61603 , vital:28041
- Description: The emergence of distributed leadership theory encourages multiple involvement of individuals in leadership of the school, regardless of their leadership positions (Spillane, 2006). The manifestation of teacher leadership through distributed leadership theory grants opportunities to teachers to enact leadership roles. This study explored how the notion of teacher leadership is understood, the leadership roles existing for the beginner teachers, and the constraining and enabling factors to the practice of teacher leadership in a rural combined school in Ohangwena region, in Namibia. The study is a formative intervention adopting a case study approach located in a critical paradigm since it aimed to bring changes in the activity system of the beginner teachers. The data were collected from ten participants: four beginner teachers, four experienced teachers, the principal and the Head of Department. The data were generated using the following techniques: document analysis, interview, questionnaires, observation and Change Laboratory workshops. The data were analysed thematically using second generation of Cultural Historical Activity Theory (CHAT) and Grant’s (2006, 2008, 2010) Teacher Leadership Model. The findings revealed that there were different understandings of the concept of teacher leadership. It was viewed as participation, influence, motivation and mentoring. It was also revealed that beginner teachers practiced leadership roles across the four zones of Grant’s (2006, 2008, 2010) Teacher Leadership Model, but to various extents. A high participation was noted in zone one within the classroom and zone two in curricular and extra-curricular activities and minimal participation was reported in zone three within the whole school development and zone four beyond the school into the community. Teacher leadership in the case study school was constrained by several factors, including teachers’ reluctance to lead, cultural beliefs, top-down leadership structure and lack of experience. Due to the minimal participation of beginner teachers in zone three and four, the findings suggested that the school should foster a collaborative culture, establish induction and mentoring committees in the school and encourage beginner teachers to take up leadership roles by providing opportunities for them to lead through delegation. In a series of Change Laboratory workshops (CLW) the principal and the Head of Department took up the responsibility of providing leadership training to the teachers to enable them to assume leadership roles in the school. In addition, participants agreed to establish induction and mentoring committees in the school to provide guidance and assistance to teacher leaders.
- Full Text:
- Date Issued: 2018
- Authors: Ndakolonkoshi, Klaudia
- Date: 2018
- Subjects: School management and organization -- Namibia , Teacher participation in administration -- Namibia , Educational leadership -- Namibia , Teacher effectiveness -- Namibia
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/61603 , vital:28041
- Description: The emergence of distributed leadership theory encourages multiple involvement of individuals in leadership of the school, regardless of their leadership positions (Spillane, 2006). The manifestation of teacher leadership through distributed leadership theory grants opportunities to teachers to enact leadership roles. This study explored how the notion of teacher leadership is understood, the leadership roles existing for the beginner teachers, and the constraining and enabling factors to the practice of teacher leadership in a rural combined school in Ohangwena region, in Namibia. The study is a formative intervention adopting a case study approach located in a critical paradigm since it aimed to bring changes in the activity system of the beginner teachers. The data were collected from ten participants: four beginner teachers, four experienced teachers, the principal and the Head of Department. The data were generated using the following techniques: document analysis, interview, questionnaires, observation and Change Laboratory workshops. The data were analysed thematically using second generation of Cultural Historical Activity Theory (CHAT) and Grant’s (2006, 2008, 2010) Teacher Leadership Model. The findings revealed that there were different understandings of the concept of teacher leadership. It was viewed as participation, influence, motivation and mentoring. It was also revealed that beginner teachers practiced leadership roles across the four zones of Grant’s (2006, 2008, 2010) Teacher Leadership Model, but to various extents. A high participation was noted in zone one within the classroom and zone two in curricular and extra-curricular activities and minimal participation was reported in zone three within the whole school development and zone four beyond the school into the community. Teacher leadership in the case study school was constrained by several factors, including teachers’ reluctance to lead, cultural beliefs, top-down leadership structure and lack of experience. Due to the minimal participation of beginner teachers in zone three and four, the findings suggested that the school should foster a collaborative culture, establish induction and mentoring committees in the school and encourage beginner teachers to take up leadership roles by providing opportunities for them to lead through delegation. In a series of Change Laboratory workshops (CLW) the principal and the Head of Department took up the responsibility of providing leadership training to the teachers to enable them to assume leadership roles in the school. In addition, participants agreed to establish induction and mentoring committees in the school to provide guidance and assistance to teacher leaders.
- Full Text:
- Date Issued: 2018
A narrative study of patients’ illness experiences on antiretroviral treatment
- Authors: Tsope, Lindiwe
- Date: 2018
- Subjects: AIDS (Disease) Social aspects South Africa , HIV infections Social aspects South Africa , Stigma (Social psychology) , Antiretroviral agents , Disclosure of information , Social media in medicine South Africa , Discourse analysis, Narrative
- Language: English
- Type: text , Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/63032 , vital:28356
- Description: Eight female respondents, who have publicly disclosed their HIV-positive status on social media, were involved in a semi-structured in-depth interview process. Using the theoretical frameworks of symbolic interactionism and social constructionism, the study explores the effects of antiretroviral treatment on patients’ illness experiences, looking at the personal and social symbolisms and meanings attached to taking antiretrovirals. The study revealed a positive and inspirational aspect of living with HIV/AIDS and especially consuming antiretroviral therapy. It became evident that the knowledge participants had of antiretrovirals before consuming them was misguided and based more on false ‘general knowledge’ among laypersons than actual medical fact. Moreover, the study revealed that there is a social reconstruction of narratives that has taken place in each participant’s life due to consuming antiretrovirals. Publicly disclosing their statuses has also proved to have both negative and positive consequences for the individuals and for society at large. While there is a consensus that participants’ illness experiences are directly affected by antiretroviral treatment, each participant’s narrative is different, yet positive.
- Full Text:
- Date Issued: 2018
- Authors: Tsope, Lindiwe
- Date: 2018
- Subjects: AIDS (Disease) Social aspects South Africa , HIV infections Social aspects South Africa , Stigma (Social psychology) , Antiretroviral agents , Disclosure of information , Social media in medicine South Africa , Discourse analysis, Narrative
- Language: English
- Type: text , Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/63032 , vital:28356
- Description: Eight female respondents, who have publicly disclosed their HIV-positive status on social media, were involved in a semi-structured in-depth interview process. Using the theoretical frameworks of symbolic interactionism and social constructionism, the study explores the effects of antiretroviral treatment on patients’ illness experiences, looking at the personal and social symbolisms and meanings attached to taking antiretrovirals. The study revealed a positive and inspirational aspect of living with HIV/AIDS and especially consuming antiretroviral therapy. It became evident that the knowledge participants had of antiretrovirals before consuming them was misguided and based more on false ‘general knowledge’ among laypersons than actual medical fact. Moreover, the study revealed that there is a social reconstruction of narratives that has taken place in each participant’s life due to consuming antiretrovirals. Publicly disclosing their statuses has also proved to have both negative and positive consequences for the individuals and for society at large. While there is a consensus that participants’ illness experiences are directly affected by antiretroviral treatment, each participant’s narrative is different, yet positive.
- Full Text:
- Date Issued: 2018
Studies in leaf domatia-mite mutualism in South Africa
- Authors: Situngu, Sivuyisiwe
- Date: 2018
- Subjects: Insect-plant relationships , Mites , Mutualism (Biology) , Biological pest control agents
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63334 , vital:28394
- Description: Plants have various traits which allow them to cope and resist their enemies including both insects and fungi . In some cases such traits allow plants to build mutualistic relationships with natural enemies of plant pests. This is the case in many dicotyledonous plants which produce leaf domatia. Leaf domatia are plant cavities usually found in the axils of major veins in the abaxial side of leaves. They are usually associated with mites and often mediate mutualistic relationships with predacious mites. Mites use leaf domatia primarily for shelter, to reproduce, and to develop. In turn, plants benefit from having predaceous mites on their leaves, because mites act as plant “bodyguards” and offer defence against pathogens and small arthropod herbivores. This phenomenon has been well documented all over the world, but Africa remains disproportionally understudied. The aim of this study was to fill the gap that exists in our knowledge of the extent of the distribution of leaf domatia-mite mutualisms and generate a better understanding of the diversity of mites found within leaf domatia from an African perspective. This was done by surveying plant species that bear leaf domatia from different vegetation types in South Africa. The plants with leaf domatia were examined for the presence of mites in order to determine patterns of mite abundance and diversity and, in so doing, address the following questions: • Does each tree species host have a specific mite or mite assemblage? • Do some mites prefer certain types of leaf domatia? • Do mites prefer a specific place in the tree canopy and does the microclimate in the tree canopy affect the distribution of mites? • Do different vegetation sites and types differ in their mite diversity and species composition? • Does mite abundance and diversity vary with seasons? Do coffee plantations have a different suite of mites than the adjacent forest? The anatomical structures of leaf domatia from six selected plant species(Coffea arabica, Gardenia thunbergia, Rothmannia capensis, Rothmannia globosa (Rubiaceae), Ocotea bullata (Lauraceae) and Tecoma capensis (Bignoniaceae) with different types of leaf domatia were also studied. The results from this study suggested that the key futures which distinguish domatia are the presence of an extra layer of tissue in the lower epidermis, a thick cuticle, cuticular folds, the presence of trichomes and an invagination. This study provides a better understating of the structure of leaf domatia. Leaf domatia bearing plants are widely distributed in South Africa, and species and vegetation-specific associations were assessed. Over 250 plant specimens with leaf domatia were collected and examined and more than 60 different mite species were found in association with the sampled plant species. The majority of mites found within the domatia of these tree species were predaceous and included mites from Stigmatidae, Tydeidae and Phytoseiidae. Furthermore, 15 new species were collected, suggesting that mites are understudied in South Africa. This study showed that the different vegetation types sampled did not differ markedly in terms of their mite biota and that similar mites were found across the region, and the association between leaf domatia and mites was found to be opportunistic and that mites had no preference for any particular domatia types. No host specificity relationship was observed between plants and mites. The assessment of mites associated with Coffea arabica showed that indigenous mites are able to colonise and establish a beneficial mutualism on exotic species. This is important as it ascertains that economically important plants that are cultivated outside their area of natural distribution can still benefit from this mutualism. This study also found that mite abundance and diversity in plants with leaf domatia were influenced by factors such as temperature, relative humidity and rainfall. Mite communities found in association with domatia changed as the year progressed and over the seasons. The seasonal fluctuations varied between the sampled plant species. In addition, this study found that mites were sensitive to extreme environmental conditions, and thus, mites preferred leaves found in the lower parts of the tree canopy and avoided exposed leaves. This study provides a better understanding of the distribution of domatia bearing plants in South Africa and their associated mites and contributes to our knowledge of the biodiversity of mites in the region. Furthermore, this study also adds to our understanding of the leaf domatia - mite mutualism in Africa. The applied example looking at the plant-mite mutualism in Coffea arabica highlights the importance of this mutualism in commercial plants.
- Full Text:
- Date Issued: 2018
- Authors: Situngu, Sivuyisiwe
- Date: 2018
- Subjects: Insect-plant relationships , Mites , Mutualism (Biology) , Biological pest control agents
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63334 , vital:28394
- Description: Plants have various traits which allow them to cope and resist their enemies including both insects and fungi . In some cases such traits allow plants to build mutualistic relationships with natural enemies of plant pests. This is the case in many dicotyledonous plants which produce leaf domatia. Leaf domatia are plant cavities usually found in the axils of major veins in the abaxial side of leaves. They are usually associated with mites and often mediate mutualistic relationships with predacious mites. Mites use leaf domatia primarily for shelter, to reproduce, and to develop. In turn, plants benefit from having predaceous mites on their leaves, because mites act as plant “bodyguards” and offer defence against pathogens and small arthropod herbivores. This phenomenon has been well documented all over the world, but Africa remains disproportionally understudied. The aim of this study was to fill the gap that exists in our knowledge of the extent of the distribution of leaf domatia-mite mutualisms and generate a better understanding of the diversity of mites found within leaf domatia from an African perspective. This was done by surveying plant species that bear leaf domatia from different vegetation types in South Africa. The plants with leaf domatia were examined for the presence of mites in order to determine patterns of mite abundance and diversity and, in so doing, address the following questions: • Does each tree species host have a specific mite or mite assemblage? • Do some mites prefer certain types of leaf domatia? • Do mites prefer a specific place in the tree canopy and does the microclimate in the tree canopy affect the distribution of mites? • Do different vegetation sites and types differ in their mite diversity and species composition? • Does mite abundance and diversity vary with seasons? Do coffee plantations have a different suite of mites than the adjacent forest? The anatomical structures of leaf domatia from six selected plant species(Coffea arabica, Gardenia thunbergia, Rothmannia capensis, Rothmannia globosa (Rubiaceae), Ocotea bullata (Lauraceae) and Tecoma capensis (Bignoniaceae) with different types of leaf domatia were also studied. The results from this study suggested that the key futures which distinguish domatia are the presence of an extra layer of tissue in the lower epidermis, a thick cuticle, cuticular folds, the presence of trichomes and an invagination. This study provides a better understating of the structure of leaf domatia. Leaf domatia bearing plants are widely distributed in South Africa, and species and vegetation-specific associations were assessed. Over 250 plant specimens with leaf domatia were collected and examined and more than 60 different mite species were found in association with the sampled plant species. The majority of mites found within the domatia of these tree species were predaceous and included mites from Stigmatidae, Tydeidae and Phytoseiidae. Furthermore, 15 new species were collected, suggesting that mites are understudied in South Africa. This study showed that the different vegetation types sampled did not differ markedly in terms of their mite biota and that similar mites were found across the region, and the association between leaf domatia and mites was found to be opportunistic and that mites had no preference for any particular domatia types. No host specificity relationship was observed between plants and mites. The assessment of mites associated with Coffea arabica showed that indigenous mites are able to colonise and establish a beneficial mutualism on exotic species. This is important as it ascertains that economically important plants that are cultivated outside their area of natural distribution can still benefit from this mutualism. This study also found that mite abundance and diversity in plants with leaf domatia were influenced by factors such as temperature, relative humidity and rainfall. Mite communities found in association with domatia changed as the year progressed and over the seasons. The seasonal fluctuations varied between the sampled plant species. In addition, this study found that mites were sensitive to extreme environmental conditions, and thus, mites preferred leaves found in the lower parts of the tree canopy and avoided exposed leaves. This study provides a better understanding of the distribution of domatia bearing plants in South Africa and their associated mites and contributes to our knowledge of the biodiversity of mites in the region. Furthermore, this study also adds to our understanding of the leaf domatia - mite mutualism in Africa. The applied example looking at the plant-mite mutualism in Coffea arabica highlights the importance of this mutualism in commercial plants.
- Full Text:
- Date Issued: 2018
Exploration potential for copperbelt - style mineralisation in NW Province, Zambia; soil geochemistry as a targeting tool
- Authors: Mwamba, John
- Date: 2018
- Subjects: Mineralogy -- Zambia -- Copperbelt Province , River sediments -- Zambia -- Copperbelt Province , Soils -- Sampling -- Zambia -- Copperbelt Province , Prospecting -- Geophysical methods , Landsat satellites , Lufilian fold belt
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62538 , vital:28204
- Description: The NW Province of Zambia is fast becoming a major significant mining district challenging to usurp the economic importance of the traditional Copperbelt Province that has been mined for nearly a century. With latest developments at Kansanshi, Lumwana and Kalumbila mines exploration efforts in the search for Copperbelt style mineralisation have doubled up in the province in recent months. Traditional methods of stream sediment and soil sampling, geophysics, aerial photo and Landsat imagery interpretations have been employed in exploration targeting campaigns. This thesis asks the question: Can we use the Copperbelt geochemical footprint as a proxy to finding new copper deposits in NW Province? The challenge faced in such studies is that few geochemical datasets for old mines exist and the little that does is proprietary information. In some mines this dataset is entirely nonexistent - at least not in the public domain. Attempting to run orientation geochemical trials on such mines is not feasible at present due to maturity of mining and the levels of contamination of the natural environment that have occurred over several decades of mining. However, in tackling this question few Copperbelt geochemical datasets from Baluba, Nkana, Mimbula, Nchanga, Bwana Mkubwa, Mufulira West and Lufubu North were used. The findings presented in this report are that for Copperbelt style mineralisation Cu/Co, Cu/Ni, Cu/Ag ratios in soil geochemistry data should be in the ranges of 0.25 to 0.48 provided geochemical studies occurred in residual soils. These ratios hold true for sediment hosted copper-cobalt mineralisation hosted at various stratigraphic levels within the Roan Group or in upper levels elsewhere on the Central African Copperbelt. Geochemical dataset for the study areas presented in this report show that the soil geochemistry footprint in the province is not dissimilar to the soil geochemistry footprint of the traditional Copperbelt Province. This means there is great potential for finding Copperbelt style mineralisation in the province and other styles of mineralisation in which copper is associated with cobalt, lead, zinc, nickel, vanadium and molybdenum. The areas of study also possess requisite geological factors that are conducive to hosting Copperbelt style deposits. These factors include: favourable structural traps with similar trends to existing mines in the province, geophysical characteristics comparable to other deposits in the province, right geological package known to host multi-type deposits in the Katangan stratigraphic sequence, and similar geochemical footprints observed on other deposits within the Lufilian fold belt. For this reason, geochemical dataset must not be looked at in isolation but should be treated in considerations with other factors and geological environment.
- Full Text:
- Date Issued: 2018
- Authors: Mwamba, John
- Date: 2018
- Subjects: Mineralogy -- Zambia -- Copperbelt Province , River sediments -- Zambia -- Copperbelt Province , Soils -- Sampling -- Zambia -- Copperbelt Province , Prospecting -- Geophysical methods , Landsat satellites , Lufilian fold belt
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62538 , vital:28204
- Description: The NW Province of Zambia is fast becoming a major significant mining district challenging to usurp the economic importance of the traditional Copperbelt Province that has been mined for nearly a century. With latest developments at Kansanshi, Lumwana and Kalumbila mines exploration efforts in the search for Copperbelt style mineralisation have doubled up in the province in recent months. Traditional methods of stream sediment and soil sampling, geophysics, aerial photo and Landsat imagery interpretations have been employed in exploration targeting campaigns. This thesis asks the question: Can we use the Copperbelt geochemical footprint as a proxy to finding new copper deposits in NW Province? The challenge faced in such studies is that few geochemical datasets for old mines exist and the little that does is proprietary information. In some mines this dataset is entirely nonexistent - at least not in the public domain. Attempting to run orientation geochemical trials on such mines is not feasible at present due to maturity of mining and the levels of contamination of the natural environment that have occurred over several decades of mining. However, in tackling this question few Copperbelt geochemical datasets from Baluba, Nkana, Mimbula, Nchanga, Bwana Mkubwa, Mufulira West and Lufubu North were used. The findings presented in this report are that for Copperbelt style mineralisation Cu/Co, Cu/Ni, Cu/Ag ratios in soil geochemistry data should be in the ranges of 0.25 to 0.48 provided geochemical studies occurred in residual soils. These ratios hold true for sediment hosted copper-cobalt mineralisation hosted at various stratigraphic levels within the Roan Group or in upper levels elsewhere on the Central African Copperbelt. Geochemical dataset for the study areas presented in this report show that the soil geochemistry footprint in the province is not dissimilar to the soil geochemistry footprint of the traditional Copperbelt Province. This means there is great potential for finding Copperbelt style mineralisation in the province and other styles of mineralisation in which copper is associated with cobalt, lead, zinc, nickel, vanadium and molybdenum. The areas of study also possess requisite geological factors that are conducive to hosting Copperbelt style deposits. These factors include: favourable structural traps with similar trends to existing mines in the province, geophysical characteristics comparable to other deposits in the province, right geological package known to host multi-type deposits in the Katangan stratigraphic sequence, and similar geochemical footprints observed on other deposits within the Lufilian fold belt. For this reason, geochemical dataset must not be looked at in isolation but should be treated in considerations with other factors and geological environment.
- Full Text:
- Date Issued: 2018
A baseline survey of channel geomorphology with particular reference to the effects of sediment characteristics on ecosystem health in the Tsitsa River, Eastern Cape, South Africa
- Huchzermeyer, Nicholaus Heinrich
- Authors: Huchzermeyer, Nicholaus Heinrich
- Date: 2018
- Subjects: Fluvial geomorphology -- South Africa -- Eastern Cape , Stream health -- South Africa -- Eastern Cape , River sediments -- South Africa -- Eastern Cape , Watershed management -- South Africa -- Eastern Cape , Dams -- Environmental aspects -- South Africa -- Eastern Cape , Tsitsa River (South Africa) , Ntabelanga Dam (South Africa)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/58056 , vital:27040
- Description: Fluvial systems are dynamic systems in which variables in a catchment and river channel affect the morphology of river reaches. South African rivers are increasingly being exposed to stresses from a combination of factors, one of the most prevalent being the impacts of damming rivers which result in varying downstream sediment fluxes and flow regimes. The sediment load combined with flow characteristics for respective river channels provides the physical habitat for aquatic ecosystems. The damming of the Tsitsa River, through the construction of the Ntabelanga Dam, will change the overall downstream geomorphology. This creates an opportunity for research in the preconstruction window. The current condition of the Tsitsa River was monitored by completing a baseline survey of the channel geomorphology with specific reference to the influence of sediment on river habitats and ecosystem health. Five sites were established in variable reaches of the Tsitsa River, with Site 1 located above the proposed Ntabelanga Dam inundation and Sites 2-5 below the proposed dam wall. Each site included a range of features that can be monitored for their response to the dam. Physical variables, water quality and biota were monitored seasonally to note changes in habitat quality. A baseline survey of the present geomorphology and associated instream habitats of the selected reaches was set up by conducting cross-sectional surveys of channel topography, water slope surveys, discharge measurements and visual and quantitative assessments of substrate. Level loggers were installed at each site to collect continuous data on variations in depth and temperature. Monitoring surveys, in terms of fine sediment accumulation, were conducted to characterise dynamic habitat arrangements and macroinvertebrate community composition. A taxa related physical habitat score for the Tsitsa River was created. The relationship between water quality, physical and ecological characteristics of the Tsitsa River will aid further research in the area as well as create a better understanding of the influence of sediment on river habitats and ecosystem health. Monitoring sites can be used to monitor the impact of catchment-wide rehabilitation on river health prior to the dam being built. After dam construction, the top site above the dam inundation can still be used as a point to monitor the impact of catchment rehabilitation on ecosystem health in terms of fine sediment accumulation.
- Full Text:
- Date Issued: 2018
- Authors: Huchzermeyer, Nicholaus Heinrich
- Date: 2018
- Subjects: Fluvial geomorphology -- South Africa -- Eastern Cape , Stream health -- South Africa -- Eastern Cape , River sediments -- South Africa -- Eastern Cape , Watershed management -- South Africa -- Eastern Cape , Dams -- Environmental aspects -- South Africa -- Eastern Cape , Tsitsa River (South Africa) , Ntabelanga Dam (South Africa)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/58056 , vital:27040
- Description: Fluvial systems are dynamic systems in which variables in a catchment and river channel affect the morphology of river reaches. South African rivers are increasingly being exposed to stresses from a combination of factors, one of the most prevalent being the impacts of damming rivers which result in varying downstream sediment fluxes and flow regimes. The sediment load combined with flow characteristics for respective river channels provides the physical habitat for aquatic ecosystems. The damming of the Tsitsa River, through the construction of the Ntabelanga Dam, will change the overall downstream geomorphology. This creates an opportunity for research in the preconstruction window. The current condition of the Tsitsa River was monitored by completing a baseline survey of the channel geomorphology with specific reference to the influence of sediment on river habitats and ecosystem health. Five sites were established in variable reaches of the Tsitsa River, with Site 1 located above the proposed Ntabelanga Dam inundation and Sites 2-5 below the proposed dam wall. Each site included a range of features that can be monitored for their response to the dam. Physical variables, water quality and biota were monitored seasonally to note changes in habitat quality. A baseline survey of the present geomorphology and associated instream habitats of the selected reaches was set up by conducting cross-sectional surveys of channel topography, water slope surveys, discharge measurements and visual and quantitative assessments of substrate. Level loggers were installed at each site to collect continuous data on variations in depth and temperature. Monitoring surveys, in terms of fine sediment accumulation, were conducted to characterise dynamic habitat arrangements and macroinvertebrate community composition. A taxa related physical habitat score for the Tsitsa River was created. The relationship between water quality, physical and ecological characteristics of the Tsitsa River will aid further research in the area as well as create a better understanding of the influence of sediment on river habitats and ecosystem health. Monitoring sites can be used to monitor the impact of catchment-wide rehabilitation on river health prior to the dam being built. After dam construction, the top site above the dam inundation can still be used as a point to monitor the impact of catchment rehabilitation on ecosystem health in terms of fine sediment accumulation.
- Full Text:
- Date Issued: 2018
Understanding the extension capacity needs of the CapeNature Stewardship Programme in the Western Cape Province of South Africa
- Coetzee, Johannes Christiaan
- Authors: Coetzee, Johannes Christiaan
- Date: 2018
- Subjects: Conservation projects (Natural resources) , Psychometrics , Adaptive natural resource management , Biodiversity conservation , CapeNature Stewardship Programme
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63220 , vital:28383
- Description: There is an increasing call for conservation programmes to provide sound evidence of effectiveness, and employing empirical evaluations can assist in the transition to evidence-based conservation practices. The objectives of this research were to develop a logic model for the CapeNature Stewardship Programme which would articulate the programme's theory of operation with respect to its Stewardship Programme landholders. The second major objective was to develop psychometric instruments for assessing the motivations and satisfactions of the programme's stewardship landholders. Both objectives included the aim to provide robust and repeatable instruments for exploring landholder's psychology, and developing a programme's theory of operation to understand the programme and improve with understanding the needs of the landowners. In this regard the processes and methodologies employed represent a major component of this research. A mixed methods approach was utilized, including stakeholder and volunteer surveys, conducted via mailing hardcopies and the internet, together with three focus groups held with the programme's management, extension staff and the stewardship landholders. Analysis of the data thus collected included both qualitative and quantitative approaches, specifically coding and content analysis, together with statistical tests of internal consistency, factor analysis and doubling correspondence analysis. Robust indices for example validity and internal consistency were developed for assessing landholder’s satisfaction with extension and level of satisfaction with the stewardship programme (Babbie 2007). These indices revealed that landholders in the Stewardship Programme are not satisfied with the programme, and exhibit behaviours suggesting they act as advocates for the programme. Demographic data and additional information provided further insights into the programme. The development of a method for articulating the programme's theory of operation is represented, together with four logic models which graphically illustrate this theory. This process and theory allowed for recommendations to be provided for the programme's improvement. A platform for adaptive management and further evaluations of this, and similar programmes, represents a major outcome of this research, understanding the extension capacity needs for the conservation of biodiversity in the CapeNature Stewardship Programme to function as a model for improving the implementation of the programme across the Western Cape, South Africa. This research feeds into an evaluation of CapeNature’s Biodiveristy Stewardship programme and demonstrates the importance of incorporating psychology into conservation interventions.
- Full Text:
- Date Issued: 2018
- Authors: Coetzee, Johannes Christiaan
- Date: 2018
- Subjects: Conservation projects (Natural resources) , Psychometrics , Adaptive natural resource management , Biodiversity conservation , CapeNature Stewardship Programme
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63220 , vital:28383
- Description: There is an increasing call for conservation programmes to provide sound evidence of effectiveness, and employing empirical evaluations can assist in the transition to evidence-based conservation practices. The objectives of this research were to develop a logic model for the CapeNature Stewardship Programme which would articulate the programme's theory of operation with respect to its Stewardship Programme landholders. The second major objective was to develop psychometric instruments for assessing the motivations and satisfactions of the programme's stewardship landholders. Both objectives included the aim to provide robust and repeatable instruments for exploring landholder's psychology, and developing a programme's theory of operation to understand the programme and improve with understanding the needs of the landowners. In this regard the processes and methodologies employed represent a major component of this research. A mixed methods approach was utilized, including stakeholder and volunteer surveys, conducted via mailing hardcopies and the internet, together with three focus groups held with the programme's management, extension staff and the stewardship landholders. Analysis of the data thus collected included both qualitative and quantitative approaches, specifically coding and content analysis, together with statistical tests of internal consistency, factor analysis and doubling correspondence analysis. Robust indices for example validity and internal consistency were developed for assessing landholder’s satisfaction with extension and level of satisfaction with the stewardship programme (Babbie 2007). These indices revealed that landholders in the Stewardship Programme are not satisfied with the programme, and exhibit behaviours suggesting they act as advocates for the programme. Demographic data and additional information provided further insights into the programme. The development of a method for articulating the programme's theory of operation is represented, together with four logic models which graphically illustrate this theory. This process and theory allowed for recommendations to be provided for the programme's improvement. A platform for adaptive management and further evaluations of this, and similar programmes, represents a major outcome of this research, understanding the extension capacity needs for the conservation of biodiversity in the CapeNature Stewardship Programme to function as a model for improving the implementation of the programme across the Western Cape, South Africa. This research feeds into an evaluation of CapeNature’s Biodiveristy Stewardship programme and demonstrates the importance of incorporating psychology into conservation interventions.
- Full Text:
- Date Issued: 2018