An analysis of the availability of and access to credit from the formal financial sector and the performance of SMEs
- Authors: Asah, Francis Tangwo
- Date: 2019
- Subjects: Small business -- South Africa -- Finance , Microfinance -- South Africa , South Africa -- Economic conditions -- 1991-
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/115138 , vital:34081
- Description: As a developing nation, South Africa faces a high rate of poverty, high levels of inequality in terms of income and a high rate of unemployment. It is officially estimated that about 27.2% of the economically active population are unemployed. SMEs are expected to be an important vehicle to address the challenges of job creation, sustainable economic growth, equitable distribution of income and the overall stimulation of economic development. SMEs in South Africa constitute 99% of all businesses, contributing to employment, income inequality and poverty alleviation (Statistic South Africa, 2018). However, despite the remarkable contribution of SMEs to the economy of South Africa, the failure rate of SMEs (at 75%) is the highest of all the efficiency-driven economies sampled by the Global Entrepreneurship Monitor (GEM). The World Bank affirm that the availability of and access to credit from the formal financial sector is the primary cause of the high failure rate of SMEs. Contemporary literature advance that the future survival and performance of SMEs in South Africa is pegged onto the amount of financial capital available to address their capital needs. Thus, this study sought to analyse the availability of and access to credit from the formal financial sector and the performance of SMEs from the supply and demand-side. In order to achieve the objectives of the study, a sequential exploratory mixed method research design, located in the pragmatic research paradigm, was used in a two phased approach. The qualitative data collection and analysis in Phase 1 informed the quantitative data collection and analysis Phase 2. In Phase 1, in-depth face-to-face semi-structured interviews were conducted with 8 credit and 8 business managers representing the sampling unit of selected formal financial institutions. The qualitative data collected was analysed using the five-steps process of content analysis as illustrated by Terre Blanche et al. (2006:322-326). The main findings with regard to factors that impact on the willingness of the formal financial sector to provide credit to SMEs were collateral, annual business turnover, audited financial records, relationship with the bank, credit profile, nature of the business, economic climate, ethics, nationality, government policy, management team, valid Identity Document/permit, equity contribution, entrepreneurship education, product quality, and business intelligence. Assessing credit applications from SMEs, risk assessment, inspection of financial records, proper documentation and background checks were the different tasks performed by credit and business managers. In addition, the main challenges faced by the formal financial sector in assessing and approving credit in favour of SMEs included the following: lack of investment capital; lack of collateral; lack of proper financial records; poor managerial knowledge; poor business plan; lack of industrial knowledge; and poor legal and credit laws. Lastly, contrary to the notion that formal financial institutions are not interested in investing in SMEs, on average, 70% of SMEs that applied for credit received such credit. In Phase 2, a self-administered questionnaire was used to collect data from formal sector owner/managers of SMEs in the city of Johannesburg. Of the 702 questionnaires distributed, 300 were returned and useable. Data was analysed using the Statistical Package for the Social Sciences (version 24). A test for normality was performed using Shapiro–Wilks test. Reliability was tested using the Cronbach’s Alpha Coefficient. Exploratory factor analysis tested the validity of factors that prevent formal financial sector from granting credit to SMEs while Binary logistic regression was used to infer on the hypotheses. Spearman’s Rho correlation analysis was used to determine whether there was any significant relationship with factors that influence access to credit and SMEs performance. The main findings revealed that access to bank finance was the only challenge that showed a significant correlation with performance. In addition, the study revealed that the South African Identity Document and collateral were the most important factors considered when applying for credit from formal financial institutions. With regard to the reasons why formal financial institutions may refuse to grant credit to SMEs, it was revealed that there was no significant positive relationship between lack of business networking and access to credit from the formal financial sector to SMEs. Conversely, the study also revealed a significant positive relationship between collateral, business information, managerial competency, business intelligence, business ethics, entrepreneurship education, legal system and macro-economy and access to credit from the formal financial sector to SMEs. Thus, it was established that there is a significant positive relationship between access to credit from the formal financial sector and the performance of SMEs.
- Full Text:
- Date Issued: 2019
- Authors: Asah, Francis Tangwo
- Date: 2019
- Subjects: Small business -- South Africa -- Finance , Microfinance -- South Africa , South Africa -- Economic conditions -- 1991-
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/115138 , vital:34081
- Description: As a developing nation, South Africa faces a high rate of poverty, high levels of inequality in terms of income and a high rate of unemployment. It is officially estimated that about 27.2% of the economically active population are unemployed. SMEs are expected to be an important vehicle to address the challenges of job creation, sustainable economic growth, equitable distribution of income and the overall stimulation of economic development. SMEs in South Africa constitute 99% of all businesses, contributing to employment, income inequality and poverty alleviation (Statistic South Africa, 2018). However, despite the remarkable contribution of SMEs to the economy of South Africa, the failure rate of SMEs (at 75%) is the highest of all the efficiency-driven economies sampled by the Global Entrepreneurship Monitor (GEM). The World Bank affirm that the availability of and access to credit from the formal financial sector is the primary cause of the high failure rate of SMEs. Contemporary literature advance that the future survival and performance of SMEs in South Africa is pegged onto the amount of financial capital available to address their capital needs. Thus, this study sought to analyse the availability of and access to credit from the formal financial sector and the performance of SMEs from the supply and demand-side. In order to achieve the objectives of the study, a sequential exploratory mixed method research design, located in the pragmatic research paradigm, was used in a two phased approach. The qualitative data collection and analysis in Phase 1 informed the quantitative data collection and analysis Phase 2. In Phase 1, in-depth face-to-face semi-structured interviews were conducted with 8 credit and 8 business managers representing the sampling unit of selected formal financial institutions. The qualitative data collected was analysed using the five-steps process of content analysis as illustrated by Terre Blanche et al. (2006:322-326). The main findings with regard to factors that impact on the willingness of the formal financial sector to provide credit to SMEs were collateral, annual business turnover, audited financial records, relationship with the bank, credit profile, nature of the business, economic climate, ethics, nationality, government policy, management team, valid Identity Document/permit, equity contribution, entrepreneurship education, product quality, and business intelligence. Assessing credit applications from SMEs, risk assessment, inspection of financial records, proper documentation and background checks were the different tasks performed by credit and business managers. In addition, the main challenges faced by the formal financial sector in assessing and approving credit in favour of SMEs included the following: lack of investment capital; lack of collateral; lack of proper financial records; poor managerial knowledge; poor business plan; lack of industrial knowledge; and poor legal and credit laws. Lastly, contrary to the notion that formal financial institutions are not interested in investing in SMEs, on average, 70% of SMEs that applied for credit received such credit. In Phase 2, a self-administered questionnaire was used to collect data from formal sector owner/managers of SMEs in the city of Johannesburg. Of the 702 questionnaires distributed, 300 were returned and useable. Data was analysed using the Statistical Package for the Social Sciences (version 24). A test for normality was performed using Shapiro–Wilks test. Reliability was tested using the Cronbach’s Alpha Coefficient. Exploratory factor analysis tested the validity of factors that prevent formal financial sector from granting credit to SMEs while Binary logistic regression was used to infer on the hypotheses. Spearman’s Rho correlation analysis was used to determine whether there was any significant relationship with factors that influence access to credit and SMEs performance. The main findings revealed that access to bank finance was the only challenge that showed a significant correlation with performance. In addition, the study revealed that the South African Identity Document and collateral were the most important factors considered when applying for credit from formal financial institutions. With regard to the reasons why formal financial institutions may refuse to grant credit to SMEs, it was revealed that there was no significant positive relationship between lack of business networking and access to credit from the formal financial sector to SMEs. Conversely, the study also revealed a significant positive relationship between collateral, business information, managerial competency, business intelligence, business ethics, entrepreneurship education, legal system and macro-economy and access to credit from the formal financial sector to SMEs. Thus, it was established that there is a significant positive relationship between access to credit from the formal financial sector and the performance of SMEs.
- Full Text:
- Date Issued: 2019
Identification of possible natural compounds as potential inhibitors against Plasmodium M1 alanyl aminopeptidase
- Soliman, Omar Samir Abdel Ghaffar
- Authors: Soliman, Omar Samir Abdel Ghaffar
- Date: 2019
- Subjects: Plasmodium , Malaria -- Chemotherapy , Plasmodium -- Inhibitors , Drug resistance in microorganisms , Aminopeptidases
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/72284 , vital:30026
- Description: Malaria is a major tropical health problem with a 29% mortality rate among people of all ages; it also affects 35% of the children. Despite the decrease in mortality rate in recent years, malaria still results in around 2000 deaths per day. Malaria is caused by Plasmodium parasites and is transmitted to humans via the bites from infected female Anopheles mosquitoes during blood meals. There are five different Plasmodium species that can cause human malaria, which include Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Plasmodium ovale and Plasmodium knowlesi. Among these five species, the most pathogenic ones are Plasmodium falciparum and Plasmodium vivax. Malaria is usually hard to diagnose because the symptoms are not exclusive to malaria and very similar to flu, e.g., fever, muscle pain, and chills, which lead to the misdiagnosis of malaria cases. Malaria is lethal if not treated because it can cause severe complications in the respiratory tract, liver, metabolic acidosis, and hypoglycemia. The malaria parasite life cycle includes two types of hosts, i.e., a human host and female Anopheles mosquito host. Malaria continuously develops resistance to the available drugs, which is one of the major challenges in disease control. This situation confirms the need to develop new drugs that target virulence factors of malaria. The malarial parasite has three main life cycle stages, which include the host liver stage, host blood stage and vector stage. In the blood stage, parasites degrade hemoglobin to amino acids, which is important as these parasites cannot produce their own amino acids. Different proteases are involved in this hemoglobin degradation process. M1 alanyl aminopeptidase is one of these proteases involved at the end of hemoglobin degradation. This study focused on M1 alanyl aminopeptidase as a potential drug target. M1 alanyl aminopeptidase consists of four domains: N-terminal domain, catalytic domain, middle domain and C-terminal domain. The catalytic domain remains conserved among different Plasmodium species. Inhibition of this enzyme might prevent Plasmodium growth as it can’t produce its own amino acids. In this study, sequence analysis was carried out in both human and Plasmodium M1 alanyl aminopeptidase to identify conserved and divergent regions between them. 3D protein models of the M1 alanyl aminopeptidase from Plasmodium species were built and validated. Then the generated models were used for virtual screening against 623 compounds retrieved from the South African Natural Compounds Database (SANCDB, https://sancdb.rubi.ru.ac.za/). Virtual screening was done using blind and targeted docking methods. Docking was used to identify compounds with selective high binding affinity to the active site of the parasite protein. In this study, one SANCDB compound was selected for each protein: SANC00531 was selected against P. falciparum M1 alanyl aminopeptidase, SANC00469 against P. knowlesi, SANC00660 against P. vivax, SANC00144 against P. ovale and SANC00109 against P. malariae. It was found that Plamsodium M1 alanyl aminopeptidase can be used as a potential drug target as it showed selective binding against different inhibitor compounds. This result will be investigated in future work though molecular dynamic analysis to investigate the stability of protein-ligand complexes.
- Full Text:
- Date Issued: 2019
- Authors: Soliman, Omar Samir Abdel Ghaffar
- Date: 2019
- Subjects: Plasmodium , Malaria -- Chemotherapy , Plasmodium -- Inhibitors , Drug resistance in microorganisms , Aminopeptidases
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/72284 , vital:30026
- Description: Malaria is a major tropical health problem with a 29% mortality rate among people of all ages; it also affects 35% of the children. Despite the decrease in mortality rate in recent years, malaria still results in around 2000 deaths per day. Malaria is caused by Plasmodium parasites and is transmitted to humans via the bites from infected female Anopheles mosquitoes during blood meals. There are five different Plasmodium species that can cause human malaria, which include Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Plasmodium ovale and Plasmodium knowlesi. Among these five species, the most pathogenic ones are Plasmodium falciparum and Plasmodium vivax. Malaria is usually hard to diagnose because the symptoms are not exclusive to malaria and very similar to flu, e.g., fever, muscle pain, and chills, which lead to the misdiagnosis of malaria cases. Malaria is lethal if not treated because it can cause severe complications in the respiratory tract, liver, metabolic acidosis, and hypoglycemia. The malaria parasite life cycle includes two types of hosts, i.e., a human host and female Anopheles mosquito host. Malaria continuously develops resistance to the available drugs, which is one of the major challenges in disease control. This situation confirms the need to develop new drugs that target virulence factors of malaria. The malarial parasite has three main life cycle stages, which include the host liver stage, host blood stage and vector stage. In the blood stage, parasites degrade hemoglobin to amino acids, which is important as these parasites cannot produce their own amino acids. Different proteases are involved in this hemoglobin degradation process. M1 alanyl aminopeptidase is one of these proteases involved at the end of hemoglobin degradation. This study focused on M1 alanyl aminopeptidase as a potential drug target. M1 alanyl aminopeptidase consists of four domains: N-terminal domain, catalytic domain, middle domain and C-terminal domain. The catalytic domain remains conserved among different Plasmodium species. Inhibition of this enzyme might prevent Plasmodium growth as it can’t produce its own amino acids. In this study, sequence analysis was carried out in both human and Plasmodium M1 alanyl aminopeptidase to identify conserved and divergent regions between them. 3D protein models of the M1 alanyl aminopeptidase from Plasmodium species were built and validated. Then the generated models were used for virtual screening against 623 compounds retrieved from the South African Natural Compounds Database (SANCDB, https://sancdb.rubi.ru.ac.za/). Virtual screening was done using blind and targeted docking methods. Docking was used to identify compounds with selective high binding affinity to the active site of the parasite protein. In this study, one SANCDB compound was selected for each protein: SANC00531 was selected against P. falciparum M1 alanyl aminopeptidase, SANC00469 against P. knowlesi, SANC00660 against P. vivax, SANC00144 against P. ovale and SANC00109 against P. malariae. It was found that Plamsodium M1 alanyl aminopeptidase can be used as a potential drug target as it showed selective binding against different inhibitor compounds. This result will be investigated in future work though molecular dynamic analysis to investigate the stability of protein-ligand complexes.
- Full Text:
- Date Issued: 2019
The Zulu girl and other poems
- Authors: Ngidi, Sandile Brian
- Date: 2019
- Subjects: Xhosa poetry
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/92748 , vital:30744
- Description: My thesis is a collection of English poems that converse with Mazisi Kunene’s literary corpus. I draw from both Kunene’s early works, which followed the pioneering work of the Zulu poet BW Vilakazi, and his socio‐political later work, which is at once Pan African in scope and intent yet deeply rooted in a Zulu socio‐linguistic milieu. Like Kunene, I aim to create a poetics of exile by working between languages, writing in Zulu and then translating into English to express my alienation in South Africa and what it means to be exiled from language and culture and speak a foreign language with your mother tongue. Here, I also take influence from the rich, evocative imagery in Palestinian poet Mahmoud Darwish's poetry of exile and the quest for a lost homeland, as well as Brazilian poet Adela Prado’s wit and direct speech. I also engage popular contemporary forms of expression and use poetry to question some themes in the popular music genre maskandi, especially its rhetoric on identity, class and gender politics.
- Full Text:
- Date Issued: 2019
- Authors: Ngidi, Sandile Brian
- Date: 2019
- Subjects: Xhosa poetry
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/92748 , vital:30744
- Description: My thesis is a collection of English poems that converse with Mazisi Kunene’s literary corpus. I draw from both Kunene’s early works, which followed the pioneering work of the Zulu poet BW Vilakazi, and his socio‐political later work, which is at once Pan African in scope and intent yet deeply rooted in a Zulu socio‐linguistic milieu. Like Kunene, I aim to create a poetics of exile by working between languages, writing in Zulu and then translating into English to express my alienation in South Africa and what it means to be exiled from language and culture and speak a foreign language with your mother tongue. Here, I also take influence from the rich, evocative imagery in Palestinian poet Mahmoud Darwish's poetry of exile and the quest for a lost homeland, as well as Brazilian poet Adela Prado’s wit and direct speech. I also engage popular contemporary forms of expression and use poetry to question some themes in the popular music genre maskandi, especially its rhetoric on identity, class and gender politics.
- Full Text:
- Date Issued: 2019
The suspended sediment yield and provenance of the Inxu River Catchment, Eastern Cape
- Authors: Nyamela, Namso
- Date: 2019
- Subjects: Suspended sediments -- South Africa -- Inxu River , Sedimentation and deposition -- South Africa -- Inxu River , Watershed management -- South Africa -- Inxu River , Erosion -- South Africa -- Inxu River , Soil erosion -- South Africa -- Inxu River
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/72150 , vital:30010
- Description: The excessive deposition and accumulation of suspended sediment leads to the degradation of water resources such as dams and rivers. For the purpose of preserving and protecting these resources, suspended sediment needs to be managed, and its management should be catchment-wide and catchment-specific. This is certainly the case for the eroded Inxu River Catchment, which has been identified as a major contributor of sediment to the planned Lalini Dam on the Tsitsa River (a tributary of the Mzimvubu River). Knowledge of suspended sediment flux and catchment sources will aid the design of relevant strategies to manage suspended sediment production. Suspended sediment flux was determined from sediment samples that were collected using citizen-based monitoring techniques at calendar- and event-based sampling frequency, along with discharges estimated using stage-discharge relationships. Sediment source areas were identified by determining the suspended sediment contribution from major sub-catchments and observing similarities in the characteristics of the sub-catchments that produced the most sediment. The Inxu River Catchment produced 5.5 t/ha/yr between 01 May 2016 and 30 April 2017. When compared to modelled sediment yield (7 t/ha/yr) from Le Roux et al. (2015), modelled output was not far off from the measured results and both measured and modelled results identified similar sediment source areas. Q-SSC relationships observed at the Inxu River Outlet indicated that sediment was eroded from local areas and sub-catchment sediment contribution confirmed that most of the sediment was from the lower Inxu River Catchment. Within this area, the Ncolosi and Qwakele River Sub-catchments were major sediment source areas and were subject to widespread gully erosion. Gully erosion was prominent on gentle slopes, foot slopes and valley-bottoms that have concave slope curvature and lie on the Tarkastad Formation. This Formation is associated with some of the most dispersive soils in the area. Moreover, the catchment is vulnerable to erosion due to cultivation and subsequent land abandonment, continuous grazing and dense rural populations. Other studies in the Mzimvubu catchment identified similar catchment characteristics that contribute to excessive erosion. This study has successfully measured sediment yield and identified areas that should be targeted and prioritised for rehabilitation within the Inxu River Catchment. The findings could be applied to a wider catchment scale. The study has successfully demonstrated the use of citizen-based monitoring and desktop techniques and has also identified some pitfalls of this approach.
- Full Text:
- Date Issued: 2019
- Authors: Nyamela, Namso
- Date: 2019
- Subjects: Suspended sediments -- South Africa -- Inxu River , Sedimentation and deposition -- South Africa -- Inxu River , Watershed management -- South Africa -- Inxu River , Erosion -- South Africa -- Inxu River , Soil erosion -- South Africa -- Inxu River
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/72150 , vital:30010
- Description: The excessive deposition and accumulation of suspended sediment leads to the degradation of water resources such as dams and rivers. For the purpose of preserving and protecting these resources, suspended sediment needs to be managed, and its management should be catchment-wide and catchment-specific. This is certainly the case for the eroded Inxu River Catchment, which has been identified as a major contributor of sediment to the planned Lalini Dam on the Tsitsa River (a tributary of the Mzimvubu River). Knowledge of suspended sediment flux and catchment sources will aid the design of relevant strategies to manage suspended sediment production. Suspended sediment flux was determined from sediment samples that were collected using citizen-based monitoring techniques at calendar- and event-based sampling frequency, along with discharges estimated using stage-discharge relationships. Sediment source areas were identified by determining the suspended sediment contribution from major sub-catchments and observing similarities in the characteristics of the sub-catchments that produced the most sediment. The Inxu River Catchment produced 5.5 t/ha/yr between 01 May 2016 and 30 April 2017. When compared to modelled sediment yield (7 t/ha/yr) from Le Roux et al. (2015), modelled output was not far off from the measured results and both measured and modelled results identified similar sediment source areas. Q-SSC relationships observed at the Inxu River Outlet indicated that sediment was eroded from local areas and sub-catchment sediment contribution confirmed that most of the sediment was from the lower Inxu River Catchment. Within this area, the Ncolosi and Qwakele River Sub-catchments were major sediment source areas and were subject to widespread gully erosion. Gully erosion was prominent on gentle slopes, foot slopes and valley-bottoms that have concave slope curvature and lie on the Tarkastad Formation. This Formation is associated with some of the most dispersive soils in the area. Moreover, the catchment is vulnerable to erosion due to cultivation and subsequent land abandonment, continuous grazing and dense rural populations. Other studies in the Mzimvubu catchment identified similar catchment characteristics that contribute to excessive erosion. This study has successfully measured sediment yield and identified areas that should be targeted and prioritised for rehabilitation within the Inxu River Catchment. The findings could be applied to a wider catchment scale. The study has successfully demonstrated the use of citizen-based monitoring and desktop techniques and has also identified some pitfalls of this approach.
- Full Text:
- Date Issued: 2019
Photophysical properties and photodynamic therapy activities of symmetrical and asymmetrical porphyrins embedded into Pluronic polymer micelles and nonlinear optical properties of an asymmetrical phthalocyanine
- Authors: Managa, Muthumuni Elizabeth
- Date: 2019
- Subjects: Porphyrins , Phthalocyanines
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/67625 , vital:29122
- Description: This work reports on the synthesis of symmetrical and asymmetrical novel porphyrins that have been incorporated into Pluronic polymers, as well as the synthesis of asymmetrical phthalocyanine. The new compounds were characterized by elemental analysis, Fourier-transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H NMR), mass spectrometry and UV–Visible spectroscopy. The porphyrins that are synthesised were not water soluble but upon incorporating into Pluronic polymer micelles, they became water soluble. The polymer was also modified and linked to folic acid, to enhance selectivity for photodynamic therapy application, where MCF7 breast cancer cells were used. The singlet oxygen quantum yields were lower for the metal free porphyrins as compared to metalled ones due to the heavy atom effect of ClGa, Zn and Cl2Si in the latter which encourages intersystem crossing to the triplet state. Singlet oxygen quantum yields for water soluble derivatives increased upon being encapsulated into the micelles for all. The Stern-Volmer constant (Ksv), binding constant (Kb) and number of binding sites (n) were investigated in order to understand the interaction between the polymer micelles and the porphyrins, and it was showed that the central metals play a role in the manner which the porphyrin interacts with the micelles. The dark toxicity and photodynamic activity of the novel porphyrins upon encapsulating to Pluronic polymer micelles is also reported. There was minimal dark toxicity for all complexes with > 90% cell survival. The photodynamic activity of water insoluble porphyrins improved when encapsulated into the micelles. Novel asymmetrical phthalocyanines were also synthesised for nonlinear optics (NLO) studies in solution and thin films.
- Full Text:
- Date Issued: 2019
- Authors: Managa, Muthumuni Elizabeth
- Date: 2019
- Subjects: Porphyrins , Phthalocyanines
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/67625 , vital:29122
- Description: This work reports on the synthesis of symmetrical and asymmetrical novel porphyrins that have been incorporated into Pluronic polymers, as well as the synthesis of asymmetrical phthalocyanine. The new compounds were characterized by elemental analysis, Fourier-transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H NMR), mass spectrometry and UV–Visible spectroscopy. The porphyrins that are synthesised were not water soluble but upon incorporating into Pluronic polymer micelles, they became water soluble. The polymer was also modified and linked to folic acid, to enhance selectivity for photodynamic therapy application, where MCF7 breast cancer cells were used. The singlet oxygen quantum yields were lower for the metal free porphyrins as compared to metalled ones due to the heavy atom effect of ClGa, Zn and Cl2Si in the latter which encourages intersystem crossing to the triplet state. Singlet oxygen quantum yields for water soluble derivatives increased upon being encapsulated into the micelles for all. The Stern-Volmer constant (Ksv), binding constant (Kb) and number of binding sites (n) were investigated in order to understand the interaction between the polymer micelles and the porphyrins, and it was showed that the central metals play a role in the manner which the porphyrin interacts with the micelles. The dark toxicity and photodynamic activity of the novel porphyrins upon encapsulating to Pluronic polymer micelles is also reported. There was minimal dark toxicity for all complexes with > 90% cell survival. The photodynamic activity of water insoluble porphyrins improved when encapsulated into the micelles. Novel asymmetrical phthalocyanines were also synthesised for nonlinear optics (NLO) studies in solution and thin films.
- Full Text:
- Date Issued: 2019
The predictors of student performance in a blended learning environment at Higher Education Institutions (HEIs) in Tanzania: a case study conducted at the University of Dar-es-Salaam
- Authors: Kabudi, Tumaini John
- Date: 2019
- Subjects: Prediction of scholastic success -- Tanzania -- Case studies , Motivation in education -- Tanzania -- Case studies , Academic achievement -- Tanzania -- Case studies , Blended learning -- Tanzania -- Case studies , Chuo Kikuu cha Dar es Salaam -- Students
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/68358 , vital:29242
- Description: Blended Learning is an important technological platform which has the potential to enhance the efficiency and efficacy of educational provision, especially in Tanzania universities. Despite a high level of investment into Blended learning, students face various challenges that have impeded them performing well in Blended learning courses. The purpose of this research is to examine and explore how student’s performance in a Blended learning environment is influenced by motivation and learning strategies; using the University of Dar es Salaam as a case study. This is a pragmatic research that utilises a mixed research design. The research design includes both qualitative design (in-depth single case study) and quantitative design (survey). The target population for this study is students participating in Blended learning modules. Primary data will be collected by means of Motivational Strategies for Learning Questionnaire and observation. It was found that motivation and learning strategies are significant predictors of student performance in a Blended learning environment. In terms of the motivation categories, Intrinsic Goal Orientation and Self-efficacy have statistically significant effect on student performance. With learning strategies sub factors, it was found out that Rehearsal, Effort Regulation and Peer Learning have significance effect on student performance. Moreover, age and gender significantly influence performance. The findings obtained are significant in building a better understanding of the influence that the mentioned predictors have on predicting the performance of students in Blended learning courses in Tanzania.
- Full Text:
- Date Issued: 2019
- Authors: Kabudi, Tumaini John
- Date: 2019
- Subjects: Prediction of scholastic success -- Tanzania -- Case studies , Motivation in education -- Tanzania -- Case studies , Academic achievement -- Tanzania -- Case studies , Blended learning -- Tanzania -- Case studies , Chuo Kikuu cha Dar es Salaam -- Students
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/68358 , vital:29242
- Description: Blended Learning is an important technological platform which has the potential to enhance the efficiency and efficacy of educational provision, especially in Tanzania universities. Despite a high level of investment into Blended learning, students face various challenges that have impeded them performing well in Blended learning courses. The purpose of this research is to examine and explore how student’s performance in a Blended learning environment is influenced by motivation and learning strategies; using the University of Dar es Salaam as a case study. This is a pragmatic research that utilises a mixed research design. The research design includes both qualitative design (in-depth single case study) and quantitative design (survey). The target population for this study is students participating in Blended learning modules. Primary data will be collected by means of Motivational Strategies for Learning Questionnaire and observation. It was found that motivation and learning strategies are significant predictors of student performance in a Blended learning environment. In terms of the motivation categories, Intrinsic Goal Orientation and Self-efficacy have statistically significant effect on student performance. With learning strategies sub factors, it was found out that Rehearsal, Effort Regulation and Peer Learning have significance effect on student performance. Moreover, age and gender significantly influence performance. The findings obtained are significant in building a better understanding of the influence that the mentioned predictors have on predicting the performance of students in Blended learning courses in Tanzania.
- Full Text:
- Date Issued: 2019
A critical analysis of A2 Fast Track Lowveld sugar cane farms in Zimbabwe in global value chains: interrogating the lives of farmers and farm labourers
- Chingono, Kudakwashe Rejoice
- Authors: Chingono, Kudakwashe Rejoice
- Date: 2019
- Subjects: International trade , Sugar trade -- Zimbabwe , Sugar growing -- Zimbabwe -- Social aspects , Agriculture -- Zimbabwe -- Social aspects
- Language: English
- Type: text , Thesis , Masters , MSocSci
- Identifier: http://hdl.handle.net/10962/93933 , vital:30972
- Description: The sugar cane industry has for many years been a lucrative business with a booming global market. In Zimbabwe, the sugar cane industry is no exception, as it has been regarded as one of the most efficient in the region and even in the world. The sugar cane farms and mills in Zimbabwe are located in Triangle and Chiredzi, in the south-eastern part of the country and they are under the ownership of Tongaat Hullet and the Zimbabwean A2 farmers. The focus of this is on the A2 fast track farms in Hippo Valley, which are now owned by black farmers but as out-growers for Tongaat Hullet. The crucial question addressed in the thesis is whether the A2 sugar cane farmers and their workers, located at the production end of the sugar cane global value chain, are benefitting from their involvement in this value chain. A number of scholars argue that global value chains lead to economic and social upgrading at the production end of the chain, based on thoughts contained in modernisation and trickledown theory. There is an assumption, then, that integration into the global economy leads to economic upgrading which translates into social upgrading. In drawing upon critical global value theorists, bolstered by the Marxist perspective, considers the importance of a more critical view of global value chains in relation to the sugar cane industry in Zimbabwe, with the particular focus on A2 farms. Thus, the main objective of the thesis is to consider the lives and livelihoods of A2 sugar cane farmers and sugar cane workers through a case study, in the context of global value chains and arguments around economic and social upgrading. This is pursued through a case study of six A2 farms, which involved interviewing farmers, supervisors, and both permanent and temporary workers. The thesis concludes that there is no significant evidence of social upgrading amongst the labour force, and that the A2 farmers are in constant tension with Tongaat Hullet in seeking to engage in economic upgrading of their status as commercial farmers.
- Full Text:
- Date Issued: 2019
- Authors: Chingono, Kudakwashe Rejoice
- Date: 2019
- Subjects: International trade , Sugar trade -- Zimbabwe , Sugar growing -- Zimbabwe -- Social aspects , Agriculture -- Zimbabwe -- Social aspects
- Language: English
- Type: text , Thesis , Masters , MSocSci
- Identifier: http://hdl.handle.net/10962/93933 , vital:30972
- Description: The sugar cane industry has for many years been a lucrative business with a booming global market. In Zimbabwe, the sugar cane industry is no exception, as it has been regarded as one of the most efficient in the region and even in the world. The sugar cane farms and mills in Zimbabwe are located in Triangle and Chiredzi, in the south-eastern part of the country and they are under the ownership of Tongaat Hullet and the Zimbabwean A2 farmers. The focus of this is on the A2 fast track farms in Hippo Valley, which are now owned by black farmers but as out-growers for Tongaat Hullet. The crucial question addressed in the thesis is whether the A2 sugar cane farmers and their workers, located at the production end of the sugar cane global value chain, are benefitting from their involvement in this value chain. A number of scholars argue that global value chains lead to economic and social upgrading at the production end of the chain, based on thoughts contained in modernisation and trickledown theory. There is an assumption, then, that integration into the global economy leads to economic upgrading which translates into social upgrading. In drawing upon critical global value theorists, bolstered by the Marxist perspective, considers the importance of a more critical view of global value chains in relation to the sugar cane industry in Zimbabwe, with the particular focus on A2 farms. Thus, the main objective of the thesis is to consider the lives and livelihoods of A2 sugar cane farmers and sugar cane workers through a case study, in the context of global value chains and arguments around economic and social upgrading. This is pursued through a case study of six A2 farms, which involved interviewing farmers, supervisors, and both permanent and temporary workers. The thesis concludes that there is no significant evidence of social upgrading amongst the labour force, and that the A2 farmers are in constant tension with Tongaat Hullet in seeking to engage in economic upgrading of their status as commercial farmers.
- Full Text:
- Date Issued: 2019
The morphological and molecular variation of southern African Nannocharax (Characiformes: Distichodontidae), and its taxonomic implications
- Authors: Smith, Timothy
- Date: 2019
- Subjects: Nannocharax -- Africa, Southern , Distichodontidae -- Africa, Southern , Freshwater fishes -- Africa, Southern , Nannocharax -- Genetics -- Africa, Southern , Distichodontidae -- Genetics -- Africa, Southern , Freshwater fishes -- Genetics -- Africa, Southern
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/69212 , vital:29446
- Description: Nannocharax is the most species rich genus in the family Distichodontidae, being currently represented by 41 species. The genus is widely distributed across much of sub-Saharan Africa, with a range extending from the Zambezi ichthyofaunal province in the south to the Nilo-Sudan ichthyofaunal province in the north. In southern Africa, the genus is currently represented by four species, Nannocharax dageti Jerep, Vari, & Vreven, 2014, N. machadoi (Poll, 1967), N. macropterus Pellegrin, 1926, and N. multifasciatus Boulenger, 1923. Each of these species exhibit considerable intraspecific pigmentation pattern variation across their respective distribution ranges, suggesting that the current taxonomy possibly underestimates the taxonomic diversity of Nannocharax species in southern Africa. Much pigmentation pattern variation within these southern African species has been observed by both collectors and scientists in the field, prompting an investigation into the extent of this morphological variation as well as what molecular variation may occur as well. The genus displays a high degree of morphological conservatisim, making it difficult to assign external morphological characters as diagnostic. To this end, this study was conducted to determine the extent of diversity of this genus in the region, employing an integrative approach with traditional morphological analysis techniques as well as sequencing the ‘barcoding gene’, cytochrome oxidase I, testing the hypothesis that there is a greater, hidden diversity of this genus in the region than currently recognised. This study aims to identify these potential lineages and accurately map their distributions. Phylogenetic analyses were performed using maximum parsimony, maximum likelihood, and Bayesian inference, using the mitochondrial cytochrome oxidase I gene region. Massive genetic divergence was detected between populations of taxa previously considered to be singular, widely distributed species. The three approaches of phylogenetic inference used in this study yielded trees of comparable overall topology, with the exception of the maximum parsimony tree which indicated additional lineages within the southern African N. multifasciatus group. These analyses revealed four deeply divergent (1.3 – 12.3%) lineages within southern African N. macropterus, as well as two deeply divergent (0.4-14.6%) populations from the Congo ichthyofaunal region, the lineages here named “N. macropterus Congo 1” and “N. macropterus Congo 2”. Within the southern African region, two deeply divergent (10.3%) lineages of N. macropterus were identified from the Okavango River system, identified as “N. macropterus Okavango 2” lineage restricted to the Cuito-Canavale tributary, and “N. macropterus Okavango 1” distributed throughout the remainder of the Okavango system. “N. macropterus Okavango 2” shares a closer relationship with the unique lineage from the Kwanza ichthyofaunal region, named N. macropterus “Kwanza”, which itself is deeply divergent from the N. macropterus “Okavango 1”, N. macropterus “Zambezi”, N. macropterus “Congo 1” and N. macropterus “Congo 2” lineages (3.1-14.4%). Principal component analyses (PCA) and discriminant function analyses (DFA) produced overlapping clusters for all identified lineages, with the exception of the N. macropterus “Kwanza” lineage, which in all analyses clustered away from the other lineages. Analysis of variance (ANOVA) and Kruskall-Wallis tests indicated significant differences in means between character traits between lineages, however, overlap in measurements and counts occurred in all instances except between the N. macropterus “Kwanza” and N. macropterus Congo lineages. However the N. macropterus “Kwanza” lineages could be distinguished from the other lineages by generally smaller fin lengths (dorsal fin 19.5%SL vs 20.0-22.1%SL in others; pectoral fin 16.5%SL vs 20.6-21.8%SL in others; pelvic fin 18.3%SL vs 21.3-22.4) and pigmentation pattern differences. The N. macropterus species group displayed extensive pigmentation pattern variation, to the extent that five pattern grades could be used to classify them. These pattern grades, while not specific to river systems, showed patterns similar to that which was seen in the molecular analyses and could be linked to lineages with only minor overlap between them. Three lineages of N. multifasciatus were identified, with two occurring in the southern African region, each corresponding to a river system, being the N. multifasciatus “Zambezi” and N. multifasciatus “Okavango” lineages. This species group displayed shallower divergence between lineages than did the N. macropterus group, at 2.5% genetic distance. Genetic analysis inferred a closer relationship between the N. multifasciatus “Zambezi” and N. multifasciatus “Congo” lineages than with the N. multifasciatus “Okavango” lineage. Morphological PCA and DFA analyses indicated morphological divergence of the N. multifasciatus “Congo” lineage, with generally larger proportional measurements than southern African specimens (body width 12.6%SL vs 9.5-9.7%SL; body depth 26.6%SL vs 21.6-21.9%SL; head width 12.0%SL vs 10.0-10.4%SL). PCA, DFA, and measurements show a near complete overlap between the N. multifasciatus “Okavango” and N. multifasciatus “Zambezi” lineages. Pigmentation pattern variation occurred within this group, but none that could be assigned to a particular lineage. The N. machadoi species group in southern Africa consists of five lineages: N. machadoi “Zambezi 1”, N. machadoi “Zambezi 2”, N. machadoi “Kafue 1”, N. machadoi “Kafue 2”, and N. machadoi “Okavango”. This group displayed shallower genetic divergence between lineages than the other southern African Nannocharax species groups (0.4-1.3%). This shallow genetic divergence is paralleled by near complete morphological overlap, with PCA and DFA producing overlapping clusters, and measurements, meristics, and pigmentation pattern metrics consisting of very similar values for the lineages. These results indicate that what is considered to be “N. macropterus” in southern Africa should not be named as such. The N. macropterus “Zambezi” and the N. macropterus “Okavango 1” lineages, are misidentifications of Nannocharax dageti. Other “N. macropterus” from the southern African region possesses fewer circumpeduncular scales than the true N. macropterus as described by Pellegrin (1926), and require taxonomic re-evaluation, each here being recognised as a unique lineage with species status, here named N. macropterus “Okavango 2” and N. macropterus “Kwanza”. In particular, N. macropterus “Kwanza” displays deep genetic divergence as well as morphological dissimilarity with the other southern African “N. macropterus” groups. Nannocharax fasciolaris and N. monardi are here placed as junior synonyms of N. multifasciatus, owing to vast overlaps in measurements and character counts of these species and N. multifasciatus, which is also known to occur within the same geographical distribution, as well as dubious arguments from the original publications in delineating these species from N. multifasciatus. Therefore, there is insufficient evidence indicating the presence of multiple species originating from the Okavango system, where it is here indicated that only a single lineage of banded, adipose fin-bearing Nannocharax occurs, namely N. multifasciatus “Okavango”.
- Full Text:
- Date Issued: 2019
- Authors: Smith, Timothy
- Date: 2019
- Subjects: Nannocharax -- Africa, Southern , Distichodontidae -- Africa, Southern , Freshwater fishes -- Africa, Southern , Nannocharax -- Genetics -- Africa, Southern , Distichodontidae -- Genetics -- Africa, Southern , Freshwater fishes -- Genetics -- Africa, Southern
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/69212 , vital:29446
- Description: Nannocharax is the most species rich genus in the family Distichodontidae, being currently represented by 41 species. The genus is widely distributed across much of sub-Saharan Africa, with a range extending from the Zambezi ichthyofaunal province in the south to the Nilo-Sudan ichthyofaunal province in the north. In southern Africa, the genus is currently represented by four species, Nannocharax dageti Jerep, Vari, & Vreven, 2014, N. machadoi (Poll, 1967), N. macropterus Pellegrin, 1926, and N. multifasciatus Boulenger, 1923. Each of these species exhibit considerable intraspecific pigmentation pattern variation across their respective distribution ranges, suggesting that the current taxonomy possibly underestimates the taxonomic diversity of Nannocharax species in southern Africa. Much pigmentation pattern variation within these southern African species has been observed by both collectors and scientists in the field, prompting an investigation into the extent of this morphological variation as well as what molecular variation may occur as well. The genus displays a high degree of morphological conservatisim, making it difficult to assign external morphological characters as diagnostic. To this end, this study was conducted to determine the extent of diversity of this genus in the region, employing an integrative approach with traditional morphological analysis techniques as well as sequencing the ‘barcoding gene’, cytochrome oxidase I, testing the hypothesis that there is a greater, hidden diversity of this genus in the region than currently recognised. This study aims to identify these potential lineages and accurately map their distributions. Phylogenetic analyses were performed using maximum parsimony, maximum likelihood, and Bayesian inference, using the mitochondrial cytochrome oxidase I gene region. Massive genetic divergence was detected between populations of taxa previously considered to be singular, widely distributed species. The three approaches of phylogenetic inference used in this study yielded trees of comparable overall topology, with the exception of the maximum parsimony tree which indicated additional lineages within the southern African N. multifasciatus group. These analyses revealed four deeply divergent (1.3 – 12.3%) lineages within southern African N. macropterus, as well as two deeply divergent (0.4-14.6%) populations from the Congo ichthyofaunal region, the lineages here named “N. macropterus Congo 1” and “N. macropterus Congo 2”. Within the southern African region, two deeply divergent (10.3%) lineages of N. macropterus were identified from the Okavango River system, identified as “N. macropterus Okavango 2” lineage restricted to the Cuito-Canavale tributary, and “N. macropterus Okavango 1” distributed throughout the remainder of the Okavango system. “N. macropterus Okavango 2” shares a closer relationship with the unique lineage from the Kwanza ichthyofaunal region, named N. macropterus “Kwanza”, which itself is deeply divergent from the N. macropterus “Okavango 1”, N. macropterus “Zambezi”, N. macropterus “Congo 1” and N. macropterus “Congo 2” lineages (3.1-14.4%). Principal component analyses (PCA) and discriminant function analyses (DFA) produced overlapping clusters for all identified lineages, with the exception of the N. macropterus “Kwanza” lineage, which in all analyses clustered away from the other lineages. Analysis of variance (ANOVA) and Kruskall-Wallis tests indicated significant differences in means between character traits between lineages, however, overlap in measurements and counts occurred in all instances except between the N. macropterus “Kwanza” and N. macropterus Congo lineages. However the N. macropterus “Kwanza” lineages could be distinguished from the other lineages by generally smaller fin lengths (dorsal fin 19.5%SL vs 20.0-22.1%SL in others; pectoral fin 16.5%SL vs 20.6-21.8%SL in others; pelvic fin 18.3%SL vs 21.3-22.4) and pigmentation pattern differences. The N. macropterus species group displayed extensive pigmentation pattern variation, to the extent that five pattern grades could be used to classify them. These pattern grades, while not specific to river systems, showed patterns similar to that which was seen in the molecular analyses and could be linked to lineages with only minor overlap between them. Three lineages of N. multifasciatus were identified, with two occurring in the southern African region, each corresponding to a river system, being the N. multifasciatus “Zambezi” and N. multifasciatus “Okavango” lineages. This species group displayed shallower divergence between lineages than did the N. macropterus group, at 2.5% genetic distance. Genetic analysis inferred a closer relationship between the N. multifasciatus “Zambezi” and N. multifasciatus “Congo” lineages than with the N. multifasciatus “Okavango” lineage. Morphological PCA and DFA analyses indicated morphological divergence of the N. multifasciatus “Congo” lineage, with generally larger proportional measurements than southern African specimens (body width 12.6%SL vs 9.5-9.7%SL; body depth 26.6%SL vs 21.6-21.9%SL; head width 12.0%SL vs 10.0-10.4%SL). PCA, DFA, and measurements show a near complete overlap between the N. multifasciatus “Okavango” and N. multifasciatus “Zambezi” lineages. Pigmentation pattern variation occurred within this group, but none that could be assigned to a particular lineage. The N. machadoi species group in southern Africa consists of five lineages: N. machadoi “Zambezi 1”, N. machadoi “Zambezi 2”, N. machadoi “Kafue 1”, N. machadoi “Kafue 2”, and N. machadoi “Okavango”. This group displayed shallower genetic divergence between lineages than the other southern African Nannocharax species groups (0.4-1.3%). This shallow genetic divergence is paralleled by near complete morphological overlap, with PCA and DFA producing overlapping clusters, and measurements, meristics, and pigmentation pattern metrics consisting of very similar values for the lineages. These results indicate that what is considered to be “N. macropterus” in southern Africa should not be named as such. The N. macropterus “Zambezi” and the N. macropterus “Okavango 1” lineages, are misidentifications of Nannocharax dageti. Other “N. macropterus” from the southern African region possesses fewer circumpeduncular scales than the true N. macropterus as described by Pellegrin (1926), and require taxonomic re-evaluation, each here being recognised as a unique lineage with species status, here named N. macropterus “Okavango 2” and N. macropterus “Kwanza”. In particular, N. macropterus “Kwanza” displays deep genetic divergence as well as morphological dissimilarity with the other southern African “N. macropterus” groups. Nannocharax fasciolaris and N. monardi are here placed as junior synonyms of N. multifasciatus, owing to vast overlaps in measurements and character counts of these species and N. multifasciatus, which is also known to occur within the same geographical distribution, as well as dubious arguments from the original publications in delineating these species from N. multifasciatus. Therefore, there is insufficient evidence indicating the presence of multiple species originating from the Okavango system, where it is here indicated that only a single lineage of banded, adipose fin-bearing Nannocharax occurs, namely N. multifasciatus “Okavango”.
- Full Text:
- Date Issued: 2019
Photophysicochemical properties and surface-enhanced Raman scattering of phthalocyanine-nanoparticle conjugates
- Authors: Nwahara, Nnamdi
- Date: 2019
- Subjects: Boron compounds , Electrochemistry , Phthalocyanines , Nanoparticles , Bioconjugates , Raman effect
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/71647 , vital:29928
- Description: This work presents the synthesis, photophysical and photochemical characterization of a series of metallophthalocyanines (MPcs) and boron dipyrromethene (BODIPY) and their conjugates with either gold or silver nanoparticles (AuNPs or AgNPs) or graphene quantum dots (GQDs). The rich π-electron systems of GQDs and MPcs employed in this work enabled the coordination of MPcs to GQDs (either as pristine or modified) via the non-covalent (π-π stacking) method. GQDs, AuNPs and AgNPs were also functionalized with L-glutathione (GSH) in order to assist coupling to the Pcs or BODIPY dye. Spectroscopic and microscopic studies confirmed the formation of the respective nanoparticles (NPs) as well as the conjugates which exhibited enhanced photophysicochemical properties in comparison to the phthalocyanines (Pcs) or BODIPY alone. This work also shows that the incorporation of folic acid (FA) into Pcs-NPs composites leads to further enhancements in the singlet oxygen generation capabilities of the resulting conjugates, and so experimentally demonstrates for the first time, a synergy between FA and the respective nanoparticles (GQDs, AuNPs and AgNPs) in affecting the photophysical properties of Pcs complexes. GQDs and Pcs/GQDs hybrids were also herein decorated with AuNPs – metallic nanostructures that employ localized surface plasmon resonances to capture or radiate electromagnetic waves at optical frequencies. These nanostructures herein reported, have been shown to possess enhanced light-matter properties, enabling unique surface-enhanced Raman scattering (SERS) behaviours, with unprecedented enhancement factors of up to 30-fold. This work therefore, reports on the fabrication of Pc/GQDs/AuNPs hybrids and experimentally demonstrates their incredible potential as novel Raman-active PDT agents.
- Full Text:
- Date Issued: 2019
- Authors: Nwahara, Nnamdi
- Date: 2019
- Subjects: Boron compounds , Electrochemistry , Phthalocyanines , Nanoparticles , Bioconjugates , Raman effect
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/71647 , vital:29928
- Description: This work presents the synthesis, photophysical and photochemical characterization of a series of metallophthalocyanines (MPcs) and boron dipyrromethene (BODIPY) and their conjugates with either gold or silver nanoparticles (AuNPs or AgNPs) or graphene quantum dots (GQDs). The rich π-electron systems of GQDs and MPcs employed in this work enabled the coordination of MPcs to GQDs (either as pristine or modified) via the non-covalent (π-π stacking) method. GQDs, AuNPs and AgNPs were also functionalized with L-glutathione (GSH) in order to assist coupling to the Pcs or BODIPY dye. Spectroscopic and microscopic studies confirmed the formation of the respective nanoparticles (NPs) as well as the conjugates which exhibited enhanced photophysicochemical properties in comparison to the phthalocyanines (Pcs) or BODIPY alone. This work also shows that the incorporation of folic acid (FA) into Pcs-NPs composites leads to further enhancements in the singlet oxygen generation capabilities of the resulting conjugates, and so experimentally demonstrates for the first time, a synergy between FA and the respective nanoparticles (GQDs, AuNPs and AgNPs) in affecting the photophysical properties of Pcs complexes. GQDs and Pcs/GQDs hybrids were also herein decorated with AuNPs – metallic nanostructures that employ localized surface plasmon resonances to capture or radiate electromagnetic waves at optical frequencies. These nanostructures herein reported, have been shown to possess enhanced light-matter properties, enabling unique surface-enhanced Raman scattering (SERS) behaviours, with unprecedented enhancement factors of up to 30-fold. This work therefore, reports on the fabrication of Pc/GQDs/AuNPs hybrids and experimentally demonstrates their incredible potential as novel Raman-active PDT agents.
- Full Text:
- Date Issued: 2019
Using a social-ecological systems approach to investigate hillslope seep wetlands ecosystem structure and functionality in the Tsitsa River catchment, Eastern Cape, South Africa
- Authors: Libala, Notiswa
- Date: 2019
- Subjects: Wetland management -- South Africa -- Eastern Cape , Wetland ecology -- South Africa -- Eastern Cape , Wetland conservation -- South Africa -- Eastern Cape , Slopes (Physical geography) -- South Africa -- Eastern Cape , Vegetation management -- South Africa -- Tsitsa River Catchment , Land degradation -- Control -- South Africa -- Tsitsa River Catchment , Grazing -- Environmental aspects -- South Africa -- Tsitsa River Catchment , Ecosystem management -- South Africa -- Tsitsa River Catchment , Wetland plants -- Effect of grazing on
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/115936 , vital:34270
- Description: Wetlands are critical ecosystems that can provide services of great social, economic and environmental value to the society. Yet, in South Africa, hillslope seep wetlands are among the most threatened ecosystems due to human-induced activities and are disappearing rapidly. Further, despite the potential vulnerability of hillslope seep wetland to disturbances, and their criticality in relation to all year round provision of forage for livestock grazing, they are among the most poorly studied wetland systems. Using a social-ecological system framing, and drawing on a range of ecological and social sciences methods, this study shed light on ways in which an integrative approach can contribute to sustainable utilisation of hillslope seep wetlands in the Tsitsa River catchment in the Eastern Cape province of South Africa. The study specific objectives were to i) evaluate the performance of Floristic Quality Assessment Index (FQAI), WET-Health and Floristic Assessment Quotient for Wetlands Index (FQAWet) indices for assessing hillslope seep wetlands ecological health ii) develop a trait-based approach for assessing the potential resilience and vulnerability of hillslope seep wetland plant species to disturbances, iii) assess the role of hillslope seep wetlands in the local communities in relation to livestock, and explore understanding of local people about the value of hillslope seep wetlands, iv) demonstrate collaborative insights emerging from an integrative social-ecological system research process to inform sustainable management of hillslope seep wetlands. A total of 11 hillslope seep wetlands were visually classified based on the level of erosion. Plant species composition within the wetlands was determined along a 100 m line transects across the hillslope wetland sites. 5 quadrats of 0.2m2 were also placed along transect for vegetation collection and cover. The plant species collected were used to calculate (FQAI) and FQAWet scores to evaluate the condition of hillslope seep wetlands. The degree of human disturbances was assessed using the Anthropogenic Activity Index (AAI), an index for qualitatively assessing the degree of human disturbance based on visual inspection of a wetland site. Factors represented in the AAI, include: (i) surrounding land use intensity; (ii) soil disturbance; (iii) hydrological alteration; (iv) habitat alteration within wetland; (v) vegetation community quality. The vegetation samples were collected in summer 2016 and winter 2017. All assessed indices were regressed against AAI to evaluate their performances. All assessed indices FQAI, FQAWet and WET-Health showed that hillslope seep wetland were impacted by human activities. FQAIall and WET-Health showed the strongest response to AAI in winter, while FQAIdom and FAQWet showed a weak response to AAI in all seasons. Overall, the findings of this study suggest that FQAIall and WET-Health are potentially better tools for assessing the biological conditions of hillslope seep wetland in South Africa. A novel trait-based approach was developed using seven plant traits and 27 trait attributes. Based on the developed approach, plant species were grouped into three potentially vulnerable groups in relation to grazing pressure. It was then predicted that species belonging to the highly vulnerable group would be less dominant at the highly disturbed sites, as well as in the winter season when grazing pressure is at its highest. The result corresponds largely with the seasonal predictions; however this was not the case for sites. The approach developed in this study worked and it was useful for predicting the potential responses of plant species in hillslope seep wetlands to grazing pressure. The success of the approach seasonally could be attributed to the careful selection of the traits, reflecting the mechanistic relationship between the grazing mode of stress on vegetation and trait-mediated biotic response. However, this still need to be refined using accurate vegetation cover methods that might have had impact on the lack of correspondence within sites. The results of the present study revealed that communities largely perceive hillslope seep wetlands as important ecosystems for their livelihoods. They recognise that the importance stems from services provided by the wetlands, particularly for livestock grazing during the dry season. Although hillslope seep wetlands are viewed as important ecosystems for livelihoods, the communities also perceive these wetlands as highly eroded ecosystems. Community members indicate willingness to strengthen local natural resource governance systems, which could lead to better management of hillslope seep wetlands. A range of protective strategies for hillslope seep were suggested by community members, including fencing, active herding and rotational grazing. The study suggests that active involvement of local communities is critical to the successful management of natural resources. The study highlights the need to consider the role of local people as influential components within social-ecological systems in order to promote effective management and conservation interventions of hillslope seep wetlands. Overall, the study highlights the criticality of an integrative social-ecological system approach for holistic management of hillslope seep wetlands within the studied catchment.
- Full Text:
- Date Issued: 2019
- Authors: Libala, Notiswa
- Date: 2019
- Subjects: Wetland management -- South Africa -- Eastern Cape , Wetland ecology -- South Africa -- Eastern Cape , Wetland conservation -- South Africa -- Eastern Cape , Slopes (Physical geography) -- South Africa -- Eastern Cape , Vegetation management -- South Africa -- Tsitsa River Catchment , Land degradation -- Control -- South Africa -- Tsitsa River Catchment , Grazing -- Environmental aspects -- South Africa -- Tsitsa River Catchment , Ecosystem management -- South Africa -- Tsitsa River Catchment , Wetland plants -- Effect of grazing on
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/115936 , vital:34270
- Description: Wetlands are critical ecosystems that can provide services of great social, economic and environmental value to the society. Yet, in South Africa, hillslope seep wetlands are among the most threatened ecosystems due to human-induced activities and are disappearing rapidly. Further, despite the potential vulnerability of hillslope seep wetland to disturbances, and their criticality in relation to all year round provision of forage for livestock grazing, they are among the most poorly studied wetland systems. Using a social-ecological system framing, and drawing on a range of ecological and social sciences methods, this study shed light on ways in which an integrative approach can contribute to sustainable utilisation of hillslope seep wetlands in the Tsitsa River catchment in the Eastern Cape province of South Africa. The study specific objectives were to i) evaluate the performance of Floristic Quality Assessment Index (FQAI), WET-Health and Floristic Assessment Quotient for Wetlands Index (FQAWet) indices for assessing hillslope seep wetlands ecological health ii) develop a trait-based approach for assessing the potential resilience and vulnerability of hillslope seep wetland plant species to disturbances, iii) assess the role of hillslope seep wetlands in the local communities in relation to livestock, and explore understanding of local people about the value of hillslope seep wetlands, iv) demonstrate collaborative insights emerging from an integrative social-ecological system research process to inform sustainable management of hillslope seep wetlands. A total of 11 hillslope seep wetlands were visually classified based on the level of erosion. Plant species composition within the wetlands was determined along a 100 m line transects across the hillslope wetland sites. 5 quadrats of 0.2m2 were also placed along transect for vegetation collection and cover. The plant species collected were used to calculate (FQAI) and FQAWet scores to evaluate the condition of hillslope seep wetlands. The degree of human disturbances was assessed using the Anthropogenic Activity Index (AAI), an index for qualitatively assessing the degree of human disturbance based on visual inspection of a wetland site. Factors represented in the AAI, include: (i) surrounding land use intensity; (ii) soil disturbance; (iii) hydrological alteration; (iv) habitat alteration within wetland; (v) vegetation community quality. The vegetation samples were collected in summer 2016 and winter 2017. All assessed indices were regressed against AAI to evaluate their performances. All assessed indices FQAI, FQAWet and WET-Health showed that hillslope seep wetland were impacted by human activities. FQAIall and WET-Health showed the strongest response to AAI in winter, while FQAIdom and FAQWet showed a weak response to AAI in all seasons. Overall, the findings of this study suggest that FQAIall and WET-Health are potentially better tools for assessing the biological conditions of hillslope seep wetland in South Africa. A novel trait-based approach was developed using seven plant traits and 27 trait attributes. Based on the developed approach, plant species were grouped into three potentially vulnerable groups in relation to grazing pressure. It was then predicted that species belonging to the highly vulnerable group would be less dominant at the highly disturbed sites, as well as in the winter season when grazing pressure is at its highest. The result corresponds largely with the seasonal predictions; however this was not the case for sites. The approach developed in this study worked and it was useful for predicting the potential responses of plant species in hillslope seep wetlands to grazing pressure. The success of the approach seasonally could be attributed to the careful selection of the traits, reflecting the mechanistic relationship between the grazing mode of stress on vegetation and trait-mediated biotic response. However, this still need to be refined using accurate vegetation cover methods that might have had impact on the lack of correspondence within sites. The results of the present study revealed that communities largely perceive hillslope seep wetlands as important ecosystems for their livelihoods. They recognise that the importance stems from services provided by the wetlands, particularly for livestock grazing during the dry season. Although hillslope seep wetlands are viewed as important ecosystems for livelihoods, the communities also perceive these wetlands as highly eroded ecosystems. Community members indicate willingness to strengthen local natural resource governance systems, which could lead to better management of hillslope seep wetlands. A range of protective strategies for hillslope seep were suggested by community members, including fencing, active herding and rotational grazing. The study suggests that active involvement of local communities is critical to the successful management of natural resources. The study highlights the need to consider the role of local people as influential components within social-ecological systems in order to promote effective management and conservation interventions of hillslope seep wetlands. Overall, the study highlights the criticality of an integrative social-ecological system approach for holistic management of hillslope seep wetlands within the studied catchment.
- Full Text:
- Date Issued: 2019
Aiding the education agenda? the role of non-governmental organisations in learner performance and retention in Joza, Grahamstown, South Africa
- Authors: Nomsenge, Sinazo Onela
- Date: 2019
- Subjects: Non-governmental organizations , Non-governmental organizations -- South Africa -- Makhanda , Black people -- Education -- South Africa -- Makhanda , Education -- South Africa -- Makhanda , Joza Youth Hub (Makhanda) , Upstart Youth Development Project (Makhanda) , Village Scribe Association (Makhanda) , Ikamba Youth (Makhanda) , Access Music Project(Makhanda)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/76468 , vital:30568
- Description: This thesis describes the network of complexities that characterise the world and work of Non-Governmental Organisations (NGOs). It examines the ways in which organisations navigate different internal, sectoral and contextual intricacies while operating under the command of their chosen developmental mandates. This description is drawn from a sociological analysis of the internal workings of education NGOs, their external affiliations as well as the negotiations which underpin their operations and survival. Collectively, the careful illustration of these underpinnings outlines both the role that NGOs play in the performance and retention of learners in the Grahamstown-east township of Joza and also their position in the town’s basic education sector. Private and non-governmental interveners have, particularly from the closing decades of the 20th century, been conceptually and operationally deployed as panaceas of the socio-economic scarcities which continue to pervade much of the ‘developing’ world. Their involvement in the socio-economic missions of populations living in the Global South has grown both laterally and in the depth of how development is understood and defined, carried out and also measured. NGOs, as widely acclaimed institutional arms of global development imperatives, therefore assume prominent positions in framing policy and implementation models, prescribing performance benchmarks and pronouncing non-compliance. Likewise, education NGOs have obtained normative prescription status in global education policy and practice largely on the back of the Education for All (EFA) objectives, the Millennium Development Goals (MDGs) and the neo-liberal logics which have championed state retreat in favour of private sector ascendance in globalised development. This, in conjunction with the persistent struggles of educational transformation in the Global South, has given NGOs little trouble in legitimising their prominent presence in education and other sectors of socio-economic relief in these parts of the world. Little illusion remains however, in many commentary circles, of the role of NGOs in advancing development ideals, the honesty of their altruistic intents, their ideological leanings as well as their efficacy in carrying out their mandates. As such, the logics which have been used to dethrone developing state structures in order to expand the space for private intervention along with the prevailing and deepening markers of educational underperformance, have been central features of the criticisms levelled against NGOs. This thesis intervenes in these ongoing reflections by describing the role of NGOs in educational outcomes, particularly learner performance and retention in Joza. This analysis demonstrates the organisational, sector-level and broader community forces which influence not only the form which non-state interventions take on and the daily preoccupations of their carriers but broadly, the position they occupy in the town’s overall educational profile. By way of locating NGOs within Grahamstown’s educational landscape, this thesis first demonstrates, the conflicted nature of NGO operations from an international, sectoral, national, local and organisational level. The discussion then illustrates how the preoccupations of NGOs are scattered between the different communities which they occupy within these levels. Their reliance on these players demands that organisations be tactical in guarding both their survival and, at times, the conflicting allegiances which grant them different forms of legitimacy. Internal struggles which characterise this imbalance of forces results in a trade-off which often favours organisational preservation mechanisms over systemic educational overhaul. As such, while non-state interveners can be lauded for extending educational support to those who would otherwise not have such, the gains of NGO intervention are often absorbed by internal urgencies for organisational legitimacy and preservation. This, in a context which possesses a unique set of socio-economic and educational deficits that require, at the very least, radical and unbridled mediation, means that pre-existing inequalities in educational inputs and outcomes along with the resultant inequities in youth socio-economic prospects, can find refuge in the very sector whose support and intervention is sought out and justified for such. This thesis lays out the nuances of these tensions and contradictions and offers this case as a point of reference for further considerations of the persistent markers of underperformance which characterise developing communities that enjoy high concentrations of non-state educational intervention.
- Full Text:
- Date Issued: 2019
- Authors: Nomsenge, Sinazo Onela
- Date: 2019
- Subjects: Non-governmental organizations , Non-governmental organizations -- South Africa -- Makhanda , Black people -- Education -- South Africa -- Makhanda , Education -- South Africa -- Makhanda , Joza Youth Hub (Makhanda) , Upstart Youth Development Project (Makhanda) , Village Scribe Association (Makhanda) , Ikamba Youth (Makhanda) , Access Music Project(Makhanda)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/76468 , vital:30568
- Description: This thesis describes the network of complexities that characterise the world and work of Non-Governmental Organisations (NGOs). It examines the ways in which organisations navigate different internal, sectoral and contextual intricacies while operating under the command of their chosen developmental mandates. This description is drawn from a sociological analysis of the internal workings of education NGOs, their external affiliations as well as the negotiations which underpin their operations and survival. Collectively, the careful illustration of these underpinnings outlines both the role that NGOs play in the performance and retention of learners in the Grahamstown-east township of Joza and also their position in the town’s basic education sector. Private and non-governmental interveners have, particularly from the closing decades of the 20th century, been conceptually and operationally deployed as panaceas of the socio-economic scarcities which continue to pervade much of the ‘developing’ world. Their involvement in the socio-economic missions of populations living in the Global South has grown both laterally and in the depth of how development is understood and defined, carried out and also measured. NGOs, as widely acclaimed institutional arms of global development imperatives, therefore assume prominent positions in framing policy and implementation models, prescribing performance benchmarks and pronouncing non-compliance. Likewise, education NGOs have obtained normative prescription status in global education policy and practice largely on the back of the Education for All (EFA) objectives, the Millennium Development Goals (MDGs) and the neo-liberal logics which have championed state retreat in favour of private sector ascendance in globalised development. This, in conjunction with the persistent struggles of educational transformation in the Global South, has given NGOs little trouble in legitimising their prominent presence in education and other sectors of socio-economic relief in these parts of the world. Little illusion remains however, in many commentary circles, of the role of NGOs in advancing development ideals, the honesty of their altruistic intents, their ideological leanings as well as their efficacy in carrying out their mandates. As such, the logics which have been used to dethrone developing state structures in order to expand the space for private intervention along with the prevailing and deepening markers of educational underperformance, have been central features of the criticisms levelled against NGOs. This thesis intervenes in these ongoing reflections by describing the role of NGOs in educational outcomes, particularly learner performance and retention in Joza. This analysis demonstrates the organisational, sector-level and broader community forces which influence not only the form which non-state interventions take on and the daily preoccupations of their carriers but broadly, the position they occupy in the town’s overall educational profile. By way of locating NGOs within Grahamstown’s educational landscape, this thesis first demonstrates, the conflicted nature of NGO operations from an international, sectoral, national, local and organisational level. The discussion then illustrates how the preoccupations of NGOs are scattered between the different communities which they occupy within these levels. Their reliance on these players demands that organisations be tactical in guarding both their survival and, at times, the conflicting allegiances which grant them different forms of legitimacy. Internal struggles which characterise this imbalance of forces results in a trade-off which often favours organisational preservation mechanisms over systemic educational overhaul. As such, while non-state interveners can be lauded for extending educational support to those who would otherwise not have such, the gains of NGO intervention are often absorbed by internal urgencies for organisational legitimacy and preservation. This, in a context which possesses a unique set of socio-economic and educational deficits that require, at the very least, radical and unbridled mediation, means that pre-existing inequalities in educational inputs and outcomes along with the resultant inequities in youth socio-economic prospects, can find refuge in the very sector whose support and intervention is sought out and justified for such. This thesis lays out the nuances of these tensions and contradictions and offers this case as a point of reference for further considerations of the persistent markers of underperformance which characterise developing communities that enjoy high concentrations of non-state educational intervention.
- Full Text:
- Date Issued: 2019
The morphological complexity of L1 Arabic-speaking children
- Authors: Issa, Iyad
- Date: 2019
- Subjects: Cognition in children , Reading , Arabic language -- Orthography and spelling , Arabic language -- Orthography and spelling -- Study and teaching , Arabic language -- Study and teaching , Arabic language -- Phonetics
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/92831 , vital:30754
- Description: Spelling poses a challenge to Arabic-speaking learners due to the complexity of the morphological and orthographic system in Arabic. Arabic morphology has been argued to play a critical role in spelling since its morphological operations are built on a system consisting of a root that is interlocking into different patterns of vowels to form different categories of words. In addition, Arabic orthography is considered to be loyal to the morphographic principle (Ravid, 2012), where morphemes correspond to graphic representation regardless of the pronunciation, especially in the non-vowelized texts. This study made a detailed classification of spelling errors in a word dictation task, based on morphological structures, undertaken by 107 Typically-developing learners (TD) and learners with learning disabiities (LD) attending the same schools. All participants ranged in age from 7 years, 3 months to 15 years, 2 months (grades 2 to 8). The spelling task was made up of 400 common words representing all morphological forms in different conjugations and grammatical classes. The results indicated that learners made three types of errors: errors with respect to the root, errors with respect to the word pattern, and errors with respect to both the root and the word pattern. The results also showed that TD and LD learners follow a similar pattern of complexity even though the LD group produced more errors than the TD group. The results revealed that MA and PA exhibited significant positive regression (b= 9.398, 16.106 respectively) with spelling, indicating that learners with higher scores in PA and MA have higher scores in spelling. The results argued for the crucial contribution that morphological awareness makes towards the general spelling abilities among learners and provide additional evidence for the nonlinear growth of morphological knowedge in spelling. In addition, spelling errors suggested that the spelling process goes in a hierarchical way where words can be accessed and processed either according to the root or according to the stem. Intact verbs are processed according to their root and word pattern. Some weak verb forms, whose radicals undergo modifications, are processed according to their stem, while those whose radicals are fully represented in the spoken word, are processed according to their root and word patterns. Therefore, roots or stems are firstly accessed and attached to basic word patterns (the grapheme without diacritics and affixes). Thereafter, prefixes and, then, suffixes are attached to the word pattern and, finally, diacritics are accessed and attached to the word pattern.
- Full Text:
- Date Issued: 2019
- Authors: Issa, Iyad
- Date: 2019
- Subjects: Cognition in children , Reading , Arabic language -- Orthography and spelling , Arabic language -- Orthography and spelling -- Study and teaching , Arabic language -- Study and teaching , Arabic language -- Phonetics
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/92831 , vital:30754
- Description: Spelling poses a challenge to Arabic-speaking learners due to the complexity of the morphological and orthographic system in Arabic. Arabic morphology has been argued to play a critical role in spelling since its morphological operations are built on a system consisting of a root that is interlocking into different patterns of vowels to form different categories of words. In addition, Arabic orthography is considered to be loyal to the morphographic principle (Ravid, 2012), where morphemes correspond to graphic representation regardless of the pronunciation, especially in the non-vowelized texts. This study made a detailed classification of spelling errors in a word dictation task, based on morphological structures, undertaken by 107 Typically-developing learners (TD) and learners with learning disabiities (LD) attending the same schools. All participants ranged in age from 7 years, 3 months to 15 years, 2 months (grades 2 to 8). The spelling task was made up of 400 common words representing all morphological forms in different conjugations and grammatical classes. The results indicated that learners made three types of errors: errors with respect to the root, errors with respect to the word pattern, and errors with respect to both the root and the word pattern. The results also showed that TD and LD learners follow a similar pattern of complexity even though the LD group produced more errors than the TD group. The results revealed that MA and PA exhibited significant positive regression (b= 9.398, 16.106 respectively) with spelling, indicating that learners with higher scores in PA and MA have higher scores in spelling. The results argued for the crucial contribution that morphological awareness makes towards the general spelling abilities among learners and provide additional evidence for the nonlinear growth of morphological knowedge in spelling. In addition, spelling errors suggested that the spelling process goes in a hierarchical way where words can be accessed and processed either according to the root or according to the stem. Intact verbs are processed according to their root and word pattern. Some weak verb forms, whose radicals undergo modifications, are processed according to their stem, while those whose radicals are fully represented in the spoken word, are processed according to their root and word patterns. Therefore, roots or stems are firstly accessed and attached to basic word patterns (the grapheme without diacritics and affixes). Thereafter, prefixes and, then, suffixes are attached to the word pattern and, finally, diacritics are accessed and attached to the word pattern.
- Full Text:
- Date Issued: 2019
Synthesis, characterisation and biological evaluation of novel anti-infective compounds bearing ferrocene, arylpyrrole, thiazolidinedione, quinoline and triazole moieties
- Authors: Oderinlo, Ogunyemi Olajide
- Date: 2019
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/114714 , vital:34016 , 10.21504/10962/114714
- Description: Expected release date-April 2022
- Full Text: false
- Date Issued: 2019
- Authors: Oderinlo, Ogunyemi Olajide
- Date: 2019
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/114714 , vital:34016 , 10.21504/10962/114714
- Description: Expected release date-April 2022
- Full Text: false
- Date Issued: 2019
Determining the hydrological functioning of the palmiet wetlands in the Eastern and Western Cape South Africa
- Authors: Smith, Caitlin
- Date: 2019
- Subjects: Wetlands -- South Africa -- Eastern Cape , Wetland ecology -- South Africa -- Eastern Cape , Wetland management -- South Africa -- Eastern Cape , Prioniaceae -- South Africa -- Eastern Cape , Prionium serratum -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/95956 , vital:31218
- Description: Wetlands provide a range of supporting, regulating and provisioning ecosystem services, including hydrological benefits such as flood attenuation and sustaining base flows. Despite their value, wetlands are one of the most vulnerable ecosystems in South Africa. Palmiet wetlands in the Eastern and Western Cape are particularly threatened. Palmiet (Prionium serratum) is a robust perennial plant that is endemic to wetlands and rivers located in the sandstones and quartzites of the Table Mountain Group (TMG), in the Eastern and Western Cape as well as the Natal Group sandstones in KwaZulu-Natal. Palmiet is described as an ecosystem engineer because of its ability to alter its environment and create large valley-bottom wetlands. The Krom River is an important water source for the city of Port Elizabeth and there has been a decline in palmiet wetlands along the Krom River as a result of alien vegetation invasion, agricultural activity, and gully erosion. Working for Water has been clearing alien vegetation and Working for Wetlands has been installing rehabilitation structures in the Krom River catchment for a number of years. There are, however, serious knowledge gaps in the understanding of palmiet wetland structure and function, particularly in respect of the hydrological functioning of these wetland systems. The aim of this study was to investigate the hydrology (surface and groundwater) behind these wetland systems. The investigation focussed on small-scale dynamics of the palmiet wetland system in order to increase general understanding of the surface water and groundwater processes of these wetland systems. Field work was concentrated on the Kompanjiesdrif and Krugersland palmiet wetlands in the upper K90A Krom River catchment. The investigation involved the installation of piezometers, water quality and stable isotope sampling and analysis, an Electrical Resistivity Tomography survey, and hydrological and mixing cell modelling. The results of the investigation indicate that the hydrological functioning of palmiet wetlands is closely linked with high sub-surface discharges typically associated with TMG aquifers. It is proposed that the palmiet wetlands are sustained by significant amounts of sub-surface water (both groundwater and interflow) moving through preferential flow paths in the alluvial fans and tributaries, which are in turn sustained by groundwater discharge from the surrounding sandstones and quartzites of the Nardouw Sub-group and Peninsula Formation. The palmiet wetlands clearly retain a significant amount of water, leading to the maintenance of prolonged flows, and a larger baseflow. However, it is hypothesised that the occurrence of palmiet as the dominant species in these wetlands is due to the sustained low flows related to catchment geology and high hydrological connectivity between the catchment and the wetland that is enabled by flow paths that allow the free flow of water from the catchment to the wetland. It is further proposed that palmiet is possibly more reliant on a consistent water supply for its existence and survival than it is on acidic nutrient-poor water and soils as stated by other authors.
- Full Text:
- Date Issued: 2019
- Authors: Smith, Caitlin
- Date: 2019
- Subjects: Wetlands -- South Africa -- Eastern Cape , Wetland ecology -- South Africa -- Eastern Cape , Wetland management -- South Africa -- Eastern Cape , Prioniaceae -- South Africa -- Eastern Cape , Prionium serratum -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/95956 , vital:31218
- Description: Wetlands provide a range of supporting, regulating and provisioning ecosystem services, including hydrological benefits such as flood attenuation and sustaining base flows. Despite their value, wetlands are one of the most vulnerable ecosystems in South Africa. Palmiet wetlands in the Eastern and Western Cape are particularly threatened. Palmiet (Prionium serratum) is a robust perennial plant that is endemic to wetlands and rivers located in the sandstones and quartzites of the Table Mountain Group (TMG), in the Eastern and Western Cape as well as the Natal Group sandstones in KwaZulu-Natal. Palmiet is described as an ecosystem engineer because of its ability to alter its environment and create large valley-bottom wetlands. The Krom River is an important water source for the city of Port Elizabeth and there has been a decline in palmiet wetlands along the Krom River as a result of alien vegetation invasion, agricultural activity, and gully erosion. Working for Water has been clearing alien vegetation and Working for Wetlands has been installing rehabilitation structures in the Krom River catchment for a number of years. There are, however, serious knowledge gaps in the understanding of palmiet wetland structure and function, particularly in respect of the hydrological functioning of these wetland systems. The aim of this study was to investigate the hydrology (surface and groundwater) behind these wetland systems. The investigation focussed on small-scale dynamics of the palmiet wetland system in order to increase general understanding of the surface water and groundwater processes of these wetland systems. Field work was concentrated on the Kompanjiesdrif and Krugersland palmiet wetlands in the upper K90A Krom River catchment. The investigation involved the installation of piezometers, water quality and stable isotope sampling and analysis, an Electrical Resistivity Tomography survey, and hydrological and mixing cell modelling. The results of the investigation indicate that the hydrological functioning of palmiet wetlands is closely linked with high sub-surface discharges typically associated with TMG aquifers. It is proposed that the palmiet wetlands are sustained by significant amounts of sub-surface water (both groundwater and interflow) moving through preferential flow paths in the alluvial fans and tributaries, which are in turn sustained by groundwater discharge from the surrounding sandstones and quartzites of the Nardouw Sub-group and Peninsula Formation. The palmiet wetlands clearly retain a significant amount of water, leading to the maintenance of prolonged flows, and a larger baseflow. However, it is hypothesised that the occurrence of palmiet as the dominant species in these wetlands is due to the sustained low flows related to catchment geology and high hydrological connectivity between the catchment and the wetland that is enabled by flow paths that allow the free flow of water from the catchment to the wetland. It is further proposed that palmiet is possibly more reliant on a consistent water supply for its existence and survival than it is on acidic nutrient-poor water and soils as stated by other authors.
- Full Text:
- Date Issued: 2019
Narratives and home: remembering an almost forgotten walk
- Bezuidenhout, Natasha Belinda
- Authors: Bezuidenhout, Natasha Belinda
- Date: 2019
- Subjects: Home in literature , Home in art , Artists -- South Africa
- Language: English
- Type: text , Thesis , Masters , MFA
- Identifier: http://hdl.handle.net/10962/115039 , vital:34072
- Description: The title of my exhibition Bittersoet alludes to the self-exploratory nature of my practice, as I interrogate the personal memories associated with objects that characterise the relationship between myself and my mother (mamma). This supporting document, Narratives and Home: Remembering an almost forgotten walk, considers the key conceptual concerns informing my practice. In this mini-thesis, I address the question: ‘What is a home?’. Drawing from my own Fine Art Practice, I explore how home can be examined as a product of the imagination, rather than only as a physical place. I consider how ‘home’ is constructed as the primary objective within an ideological framework defined by history, memory and narrative. Engaging beyond the idea of ‘home’ as a fixed structure or place, I examine the idea of ‘home’ as something fluid that is negotiated and defined by the interaction between objects and language. It is concerned with dialectics of memory and narrative as they pertain directly to an experience of both searching for and reimagining home through metaphorical representations. In particular, I explore how home can be seen as equally familiar and unfamiliar, existing in-between, always changing, never fixed, rather in a constant state of flux. The concept of home is addressed in a dialogical process by using Afrikaans as my mother tongue, I narrate informal conversations between myself and my mother. These conversations transform and expand into hybrid words, memories and narratives to form a layered continuous dialogue between my practice and research. This notion relates to exploring oneself and the ‘fictions’ of the past. The self being fundamental to the individual comprehension of both ‘place’ and ‘space’.
- Full Text:
- Date Issued: 2019
- Authors: Bezuidenhout, Natasha Belinda
- Date: 2019
- Subjects: Home in literature , Home in art , Artists -- South Africa
- Language: English
- Type: text , Thesis , Masters , MFA
- Identifier: http://hdl.handle.net/10962/115039 , vital:34072
- Description: The title of my exhibition Bittersoet alludes to the self-exploratory nature of my practice, as I interrogate the personal memories associated with objects that characterise the relationship between myself and my mother (mamma). This supporting document, Narratives and Home: Remembering an almost forgotten walk, considers the key conceptual concerns informing my practice. In this mini-thesis, I address the question: ‘What is a home?’. Drawing from my own Fine Art Practice, I explore how home can be examined as a product of the imagination, rather than only as a physical place. I consider how ‘home’ is constructed as the primary objective within an ideological framework defined by history, memory and narrative. Engaging beyond the idea of ‘home’ as a fixed structure or place, I examine the idea of ‘home’ as something fluid that is negotiated and defined by the interaction between objects and language. It is concerned with dialectics of memory and narrative as they pertain directly to an experience of both searching for and reimagining home through metaphorical representations. In particular, I explore how home can be seen as equally familiar and unfamiliar, existing in-between, always changing, never fixed, rather in a constant state of flux. The concept of home is addressed in a dialogical process by using Afrikaans as my mother tongue, I narrate informal conversations between myself and my mother. These conversations transform and expand into hybrid words, memories and narratives to form a layered continuous dialogue between my practice and research. This notion relates to exploring oneself and the ‘fictions’ of the past. The self being fundamental to the individual comprehension of both ‘place’ and ‘space’.
- Full Text:
- Date Issued: 2019
An analysis of the roles and functions of teachers’ gestures as visualisation tools in the teaching of mathematics at the junior primary phase (Grades 0 –3)
- Authors: Namakalu, Dietlinde Nelao
- Date: 2019
- Subjects: Gesture , Gesture in mathematics education , Visual learning , Visualization , Education, Elementary -- Namibia
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/96713 , vital:31311
- Description: There is ample evidence in the literature that gestures are important hand and bodily actions that play vital roles during interactions between learners and teachers in the mathematics classroom. These gestures enable both the teacher and the learner to visualise mathematical concepts and ideas. The aim of this Namibian interpretive case study, located in enactivist theory, was to analyse the types of gestures that three selected teachers at the junior primary phase (Grades 0–3) used in their teaching of mathematics. The study also interrogated the roles that these gestures played in the teaching process of the three teachers. As I intended to analyse the types of gestures the selected teachers used and understand their views and perceptions of the roles and functions their gestures played as visualisation tools in the teaching of mathematics, I observed ten lessons of each teacher and video recorded them. I then interviewed them (one-on-one stimulus-recall interviews) and interpreted the gestures they used and utterances they made about using these gestures. The types of gestures that the participating teachers used are classified according to McNeill‘s (1992) framework, namely pointing (deictic) gestures, iconic (illustrators) gestures, metaphoric gestures, beat (motor) gestures and symbolic (emblems) gestures. The study found that the participating teachers incorporated a variety of gestures into their lessons and used them strategically. According to the teachers, gestures made the lessons interesting and encouraged active participation of the learners in the lessons. The teachers also revealed that gestures assist in explaining mathematical concepts and thus affected the learners‘ understanding positively. This study strongly suggests that the usefulness and efficacy of employing gestures as visualisation tools in mathematics education should be strategically harnessed.
- Full Text:
- Date Issued: 2019
- Authors: Namakalu, Dietlinde Nelao
- Date: 2019
- Subjects: Gesture , Gesture in mathematics education , Visual learning , Visualization , Education, Elementary -- Namibia
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/96713 , vital:31311
- Description: There is ample evidence in the literature that gestures are important hand and bodily actions that play vital roles during interactions between learners and teachers in the mathematics classroom. These gestures enable both the teacher and the learner to visualise mathematical concepts and ideas. The aim of this Namibian interpretive case study, located in enactivist theory, was to analyse the types of gestures that three selected teachers at the junior primary phase (Grades 0–3) used in their teaching of mathematics. The study also interrogated the roles that these gestures played in the teaching process of the three teachers. As I intended to analyse the types of gestures the selected teachers used and understand their views and perceptions of the roles and functions their gestures played as visualisation tools in the teaching of mathematics, I observed ten lessons of each teacher and video recorded them. I then interviewed them (one-on-one stimulus-recall interviews) and interpreted the gestures they used and utterances they made about using these gestures. The types of gestures that the participating teachers used are classified according to McNeill‘s (1992) framework, namely pointing (deictic) gestures, iconic (illustrators) gestures, metaphoric gestures, beat (motor) gestures and symbolic (emblems) gestures. The study found that the participating teachers incorporated a variety of gestures into their lessons and used them strategically. According to the teachers, gestures made the lessons interesting and encouraged active participation of the learners in the lessons. The teachers also revealed that gestures assist in explaining mathematical concepts and thus affected the learners‘ understanding positively. This study strongly suggests that the usefulness and efficacy of employing gestures as visualisation tools in mathematics education should be strategically harnessed.
- Full Text:
- Date Issued: 2019
Investigating assay formats for screening malaria Hsp90-Hop interaction inhibitors
- Authors: Derry, Leigh-Anne Tracy Kim
- Date: 2019
- Subjects: Antimalarials , Heat shock proteins , Drug interactions , Drug resistance , Plasmodium falciparum , High throughput screening (Drug development) , Bioluminescence resonance energy transfer (BRET) , Fluorescence resonance energy transfer (FRET)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63345 , vital:28395
- Description: Although significant gains have been made in the combat against malaria in the last decade, the persistent threat of drug and insecticide resistance continues to motivate the search for new classes of antimalarial drug compounds and targets. Due to their predominance in cellular reactions, protein-protein interactions (P-PIs) are emerging as a promising general target class for therapeutic development. The P-PI which is the focus of this project is the interaction between the chaperone heat shock protein 90 (Hsp90) and its co-chaperone Hsp70/Hsp90 organising protein (Hop). Hop binds to Hsp70 and Hsp90 and facilitates the transfer of client proteins (proteins undergoing folding) from the former to the latter and also regulates nucleotide exchange on Hsp90. Due to its role in correcting protein misfolding during cell stress, Hsp90 is being pursued as a cancer drug target and compounds that inhibit its ATPase activity have entered clinical trials. However, it has been proposed that inhibiting the interaction between Hsp90 and Hop may be alternative approach for inhibiting Hsp90 function for cancer therapy. The malaria parasite Plasmodium falciparum experiences temperature fluctuations during vector-host transitions and febrile episodes and cell stress due to rapid growth and immune responses. Hence, it also depends on chaperones, including PfHsp90, to maintain protein functionality and pathogenesis, demonstrated inter alia by the sensitivity of parasites to Hsp90 inhibitors. In addition, PfHsp90 exists as a complex with the malarial Hop homologue, PfHop, in parasite lysates. Consequently, the purpose of this study was to explore P-PI assay formats that can confirm the interaction of PfHsp90 and PfHop and can be used to identify inhibitors of the interaction, preferably in a medium- to high-throughput screening mode. As a first approach, cell-based bioluminescence and fluorescence resonance energy transfer (BRET and FRET) assays were performed in HeLa cells. To facilitate this, expression plasmid constructs containing coding sequences of P. falciparum and mammalian Hsp90 and Hop and their interacting domains (Hsp90 C-domain and Hop TPR2A domain) fused to the BRET and FRET reporter proteins – yellow fluorescent protein (YFP), cyan fluorescent protein (CFP) and Renilla luciferase (Rluc) - were prepared and used for HeLa cell transient transfections. The FRET assay produced positive interaction signals for the full-length P. falciparum and mammalian Hsp90-Hop interactions. However, C-domain-TPR2A domain interactions were not detected, no interactions could be demonstrated with the BRET assay and western blotting experiments failed to detect expression of all the interaction partners in transiently transfected HeLa cells. Consequently, an alternative in vitro FRET assay format using recombinant proteins was investigated. Expression constructs for the P. falciparum and mammalian C-domains and TPR2A domains fused respectively to YFP and CFP were prepared and the corresponding fusion proteins expressed and purified from E. coli. No interaction was found with the mammalian interaction partners, but interaction of the P. falciparum C-domain and TPR2A domain was consistently detected with a robust Z’ factor value of 0.54. A peptide corresponding to the PfTPR2A domain sequence primarily responsible for Hsp90 binding (based on a human TPR2A peptide described by Horibe et al., 2011) was designed and showed dose-dependent inhibition of the interaction, with 53.7% inhibition at 100 μM. The components of the assay are limited to the purified recombinant proteins, requires minimal liquid steps and may thus be a useful primary screening format for identifying inhibitors of P. falciparum Hsp90-Hop interaction.
- Full Text:
- Date Issued: 2019
- Authors: Derry, Leigh-Anne Tracy Kim
- Date: 2019
- Subjects: Antimalarials , Heat shock proteins , Drug interactions , Drug resistance , Plasmodium falciparum , High throughput screening (Drug development) , Bioluminescence resonance energy transfer (BRET) , Fluorescence resonance energy transfer (FRET)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63345 , vital:28395
- Description: Although significant gains have been made in the combat against malaria in the last decade, the persistent threat of drug and insecticide resistance continues to motivate the search for new classes of antimalarial drug compounds and targets. Due to their predominance in cellular reactions, protein-protein interactions (P-PIs) are emerging as a promising general target class for therapeutic development. The P-PI which is the focus of this project is the interaction between the chaperone heat shock protein 90 (Hsp90) and its co-chaperone Hsp70/Hsp90 organising protein (Hop). Hop binds to Hsp70 and Hsp90 and facilitates the transfer of client proteins (proteins undergoing folding) from the former to the latter and also regulates nucleotide exchange on Hsp90. Due to its role in correcting protein misfolding during cell stress, Hsp90 is being pursued as a cancer drug target and compounds that inhibit its ATPase activity have entered clinical trials. However, it has been proposed that inhibiting the interaction between Hsp90 and Hop may be alternative approach for inhibiting Hsp90 function for cancer therapy. The malaria parasite Plasmodium falciparum experiences temperature fluctuations during vector-host transitions and febrile episodes and cell stress due to rapid growth and immune responses. Hence, it also depends on chaperones, including PfHsp90, to maintain protein functionality and pathogenesis, demonstrated inter alia by the sensitivity of parasites to Hsp90 inhibitors. In addition, PfHsp90 exists as a complex with the malarial Hop homologue, PfHop, in parasite lysates. Consequently, the purpose of this study was to explore P-PI assay formats that can confirm the interaction of PfHsp90 and PfHop and can be used to identify inhibitors of the interaction, preferably in a medium- to high-throughput screening mode. As a first approach, cell-based bioluminescence and fluorescence resonance energy transfer (BRET and FRET) assays were performed in HeLa cells. To facilitate this, expression plasmid constructs containing coding sequences of P. falciparum and mammalian Hsp90 and Hop and their interacting domains (Hsp90 C-domain and Hop TPR2A domain) fused to the BRET and FRET reporter proteins – yellow fluorescent protein (YFP), cyan fluorescent protein (CFP) and Renilla luciferase (Rluc) - were prepared and used for HeLa cell transient transfections. The FRET assay produced positive interaction signals for the full-length P. falciparum and mammalian Hsp90-Hop interactions. However, C-domain-TPR2A domain interactions were not detected, no interactions could be demonstrated with the BRET assay and western blotting experiments failed to detect expression of all the interaction partners in transiently transfected HeLa cells. Consequently, an alternative in vitro FRET assay format using recombinant proteins was investigated. Expression constructs for the P. falciparum and mammalian C-domains and TPR2A domains fused respectively to YFP and CFP were prepared and the corresponding fusion proteins expressed and purified from E. coli. No interaction was found with the mammalian interaction partners, but interaction of the P. falciparum C-domain and TPR2A domain was consistently detected with a robust Z’ factor value of 0.54. A peptide corresponding to the PfTPR2A domain sequence primarily responsible for Hsp90 binding (based on a human TPR2A peptide described by Horibe et al., 2011) was designed and showed dose-dependent inhibition of the interaction, with 53.7% inhibition at 100 μM. The components of the assay are limited to the purified recombinant proteins, requires minimal liquid steps and may thus be a useful primary screening format for identifying inhibitors of P. falciparum Hsp90-Hop interaction.
- Full Text:
- Date Issued: 2019
The impact of unanticipated news announcements by the US Federal Reserve On South African stock returns
- Authors: Sibanda, Lorna
- Date: 2019
- Subjects: Monetary policy -- United States , International finance , South Africa -- Foreign economic relations -- United States , United States -- Foreign economic relations -- South Africa , Banks of issue -- United States , Investments -- South Africa , Stocks -- Prices -- South Africa , Stocks -- Rate of return , Rate of return -- South Africa
- Language: English
- Type: text , Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10962/94703 , vital:31070
- Description: This thesis analyses whether monetary policy announcement shocks are transmitted across countries, with special emphasis on the impact of US Federal Reserve announcements on the South African stock market. Monetary policy is an important source of economic news and affects the risk perceptions of market participants. This study will improve the understanding of stock price determinants and possibly influence SA monetary policy in guarding against possible shocks originating from abroad. Using Federal Reserve Open Market Committee (FOMC) announcements over the period 2008 – 2014, the research studied changes in volatility of the South African FTSE/JSE All Share Index returns over this period. An event study and GARCH model approach was adopted to reach the goals of the analysis. The findings were a statistically insignificant connection between SA stock returns and both anticipated and unanticipated US Federal Reserve announcements. Over the sample period, each shock to SA stock returns persisted for approximately 4-5 months. Although SA stock return volatility demonstrated clustering behaviour (indicating sensitivity to economic shocks), the research could not find an obvious relationship between these spikes in volatility and US Federal Reserve announcements. It is concluded that South African stock returns do not change in response to unexpected US monetary policy announcements.
- Full Text:
- Date Issued: 2019
- Authors: Sibanda, Lorna
- Date: 2019
- Subjects: Monetary policy -- United States , International finance , South Africa -- Foreign economic relations -- United States , United States -- Foreign economic relations -- South Africa , Banks of issue -- United States , Investments -- South Africa , Stocks -- Prices -- South Africa , Stocks -- Rate of return , Rate of return -- South Africa
- Language: English
- Type: text , Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10962/94703 , vital:31070
- Description: This thesis analyses whether monetary policy announcement shocks are transmitted across countries, with special emphasis on the impact of US Federal Reserve announcements on the South African stock market. Monetary policy is an important source of economic news and affects the risk perceptions of market participants. This study will improve the understanding of stock price determinants and possibly influence SA monetary policy in guarding against possible shocks originating from abroad. Using Federal Reserve Open Market Committee (FOMC) announcements over the period 2008 – 2014, the research studied changes in volatility of the South African FTSE/JSE All Share Index returns over this period. An event study and GARCH model approach was adopted to reach the goals of the analysis. The findings were a statistically insignificant connection between SA stock returns and both anticipated and unanticipated US Federal Reserve announcements. Over the sample period, each shock to SA stock returns persisted for approximately 4-5 months. Although SA stock return volatility demonstrated clustering behaviour (indicating sensitivity to economic shocks), the research could not find an obvious relationship between these spikes in volatility and US Federal Reserve announcements. It is concluded that South African stock returns do not change in response to unexpected US monetary policy announcements.
- Full Text:
- Date Issued: 2019
The use of filter-feeding fish (Clarias gariepinus and Oreochromis mossambicus) to remove microalgae from brewery effluent treatment ponds
- Authors: Nombembe, Lwazi
- Date: 2019
- Subjects: Clarias gariepinus -- Food , Mozambique tilapia -- Food , Water -- Purification -- South Africa , Algae -- Biotechnology -- South Africa , Microalgae -- Biotechnology-- South Africa , Brewery waste -- South Africa , Fish culture -- South Africa
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/95745 , vital:31194
- Description: The removal of microalgae from high rate algal ponds (HRAP) in waste-water treatment systems remains a constraint to their use in effluent treatment systems. Conventional algae harvesting methods often have high energy demands, take up lots of space, are expensive to operate or are time consuming. The aim of the study was to determine if fish such as Clarias gariepinus and Oreochromis mossambicus, could be used to remove microalgae from waste-water treatment ponds (in the absence/presence of a flocculent in the former and in the absence or presence of pH moderation in the latter), and to investigate the subsequent influence of algae concentration on several water quality parameters. The age of Clarias gariepinus (3-12 months) had a positive relationship with the distance between gill rakers (98.27 to 163.34 μm; y=90.576+4.823*x: R²=0.549; F(1,18)=21.867; p<0.001) and these data suggested that these fish might be efficient at removing algae from HRAP effluent. However, this was not the case, even with flocculent application (but this result might have been confounded by very high pH readings, at which flocculation is less likely to occur). Oreochromis mossambicus removed some of this algae, but the pH was too high for tilapia culture. It was not possible to moderate the increase in pH by keeping tanks in the dark and thus preventing photosynthesis; but pH fluctuation in HRAP effluent could be moderated using CO2 sparging in an attempt to make the environment more hospitable for tilapia (the average pH that was moderated with CO2 was 8.43±0.06, whereas the unmoderated average was 10.65±0.06). However, pH moderation using CO2 sparging did not increase the rate at which algae were removed by O. mossambicus; rather, it compromised O2 concentration which dropped to 4.17±1.26 mg/l after five hours of CO2 sparging, whereas it increased to 20.50±1.41 mg/l in treatments with unadjusted pH over the same period. Fish can be used to remove algae from treated effluent, and Oreochromis mossambicus remains a recommended species. Future work needs to investigate moderating fluctuations in pH and O2 concentration to further facilitate this method of algae removal.
- Full Text:
- Date Issued: 2019
- Authors: Nombembe, Lwazi
- Date: 2019
- Subjects: Clarias gariepinus -- Food , Mozambique tilapia -- Food , Water -- Purification -- South Africa , Algae -- Biotechnology -- South Africa , Microalgae -- Biotechnology-- South Africa , Brewery waste -- South Africa , Fish culture -- South Africa
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/95745 , vital:31194
- Description: The removal of microalgae from high rate algal ponds (HRAP) in waste-water treatment systems remains a constraint to their use in effluent treatment systems. Conventional algae harvesting methods often have high energy demands, take up lots of space, are expensive to operate or are time consuming. The aim of the study was to determine if fish such as Clarias gariepinus and Oreochromis mossambicus, could be used to remove microalgae from waste-water treatment ponds (in the absence/presence of a flocculent in the former and in the absence or presence of pH moderation in the latter), and to investigate the subsequent influence of algae concentration on several water quality parameters. The age of Clarias gariepinus (3-12 months) had a positive relationship with the distance between gill rakers (98.27 to 163.34 μm; y=90.576+4.823*x: R²=0.549; F(1,18)=21.867; p<0.001) and these data suggested that these fish might be efficient at removing algae from HRAP effluent. However, this was not the case, even with flocculent application (but this result might have been confounded by very high pH readings, at which flocculation is less likely to occur). Oreochromis mossambicus removed some of this algae, but the pH was too high for tilapia culture. It was not possible to moderate the increase in pH by keeping tanks in the dark and thus preventing photosynthesis; but pH fluctuation in HRAP effluent could be moderated using CO2 sparging in an attempt to make the environment more hospitable for tilapia (the average pH that was moderated with CO2 was 8.43±0.06, whereas the unmoderated average was 10.65±0.06). However, pH moderation using CO2 sparging did not increase the rate at which algae were removed by O. mossambicus; rather, it compromised O2 concentration which dropped to 4.17±1.26 mg/l after five hours of CO2 sparging, whereas it increased to 20.50±1.41 mg/l in treatments with unadjusted pH over the same period. Fish can be used to remove algae from treated effluent, and Oreochromis mossambicus remains a recommended species. Future work needs to investigate moderating fluctuations in pH and O2 concentration to further facilitate this method of algae removal.
- Full Text:
- Date Issued: 2019
The time course changes in selected fatigue indicators in moderately trained participants
- Authors: Maduna, Bongani Cyprian
- Date: 2019
- Subjects: Athletes -- Health and hygiene , Fatigue , Athletes -- Physiology
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/76354 , vital:30553
- Description: Background: During daily physical activities, individuals will encounter some level of fatigue. This is especially true for athletes who exert their bodies to achieve superior performance and attempt to delay the onset of fatigue as far as possible. However, fatigue is a progressive process that is part of physical exercise. It is therefore, important to understand the various factors associated with fatigue. Objective: The purpose of this research project was to observe the changes in perceptual, physiological responses, and workload while participants performed a fatiguing cycling exercise task. In order to observe the aforementioned changes, one of the three variables, either perceptual, physiological responses or workload was kept constant, while the other two were observed as dependent variables. There were three test conditions, which permitted each variable to be kept constant in at least one of the three test conditions. Methods: Thirty-six apparently healthy university students were recruited for the study. The exercise was of 35 minute duration for each condition, with the data collection for HR (physiological response), RPE (perceptual response), and power output (workload) occurring at two minute intervals from the eighth minute until the thirtieth minute. The participants were required to perform a peak-power-output test in order set the relative performance ranges for each participant in order to elicit an observable fatigue response from all the participants. The three conditions included constant HR response, constant workload, and constant RPE response where each participant performed all of the above conditions on separate days.Results: The perceptual (RPE) response increased significantly (p<0.05) over time in all three test conditions, even under the constant RPE condition where it was expected to remain unchanged throughout the test duration. The HR response only demonstrated a significant (p<0.05) increase over time under the constant workload condition. Lastly, workload remained constant in all three testing conditions. Conclusion: Participants may be able to exercise for longer periods during sub-maximal exercise if they disregard the RPE warning response. In the current study, the RPE response illustrated that participants were being exerted more over time; however, the participants still had physiological and workload capacity to continue exercising. This research project has confirmed the fact that fatigue is a multifaceted phenomenon. Furthermore, it has been illustrated that RPE response alone as an indicator of fatigue onset may be misleading as participants did not breach the HR and workload steady state during the current research. Therefore, it may be more appropriate to assess fatigue onset through the assessment of more than one fatigue variable in order to ensure increased accuracy of the participants’ fatigue state assessment.
- Full Text:
- Date Issued: 2019
- Authors: Maduna, Bongani Cyprian
- Date: 2019
- Subjects: Athletes -- Health and hygiene , Fatigue , Athletes -- Physiology
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/76354 , vital:30553
- Description: Background: During daily physical activities, individuals will encounter some level of fatigue. This is especially true for athletes who exert their bodies to achieve superior performance and attempt to delay the onset of fatigue as far as possible. However, fatigue is a progressive process that is part of physical exercise. It is therefore, important to understand the various factors associated with fatigue. Objective: The purpose of this research project was to observe the changes in perceptual, physiological responses, and workload while participants performed a fatiguing cycling exercise task. In order to observe the aforementioned changes, one of the three variables, either perceptual, physiological responses or workload was kept constant, while the other two were observed as dependent variables. There were three test conditions, which permitted each variable to be kept constant in at least one of the three test conditions. Methods: Thirty-six apparently healthy university students were recruited for the study. The exercise was of 35 minute duration for each condition, with the data collection for HR (physiological response), RPE (perceptual response), and power output (workload) occurring at two minute intervals from the eighth minute until the thirtieth minute. The participants were required to perform a peak-power-output test in order set the relative performance ranges for each participant in order to elicit an observable fatigue response from all the participants. The three conditions included constant HR response, constant workload, and constant RPE response where each participant performed all of the above conditions on separate days.Results: The perceptual (RPE) response increased significantly (p<0.05) over time in all three test conditions, even under the constant RPE condition where it was expected to remain unchanged throughout the test duration. The HR response only demonstrated a significant (p<0.05) increase over time under the constant workload condition. Lastly, workload remained constant in all three testing conditions. Conclusion: Participants may be able to exercise for longer periods during sub-maximal exercise if they disregard the RPE warning response. In the current study, the RPE response illustrated that participants were being exerted more over time; however, the participants still had physiological and workload capacity to continue exercising. This research project has confirmed the fact that fatigue is a multifaceted phenomenon. Furthermore, it has been illustrated that RPE response alone as an indicator of fatigue onset may be misleading as participants did not breach the HR and workload steady state during the current research. Therefore, it may be more appropriate to assess fatigue onset through the assessment of more than one fatigue variable in order to ensure increased accuracy of the participants’ fatigue state assessment.
- Full Text:
- Date Issued: 2019