Using a social-ecological systems approach to investigate hillslope seep wetlands ecosystem structure and functionality in the Tsitsa River catchment, Eastern Cape, South Africa
- Authors: Libala, Notiswa
- Date: 2019
- Subjects: Wetland management -- South Africa -- Eastern Cape , Wetland ecology -- South Africa -- Eastern Cape , Wetland conservation -- South Africa -- Eastern Cape , Slopes (Physical geography) -- South Africa -- Eastern Cape , Vegetation management -- South Africa -- Tsitsa River Catchment , Land degradation -- Control -- South Africa -- Tsitsa River Catchment , Grazing -- Environmental aspects -- South Africa -- Tsitsa River Catchment , Ecosystem management -- South Africa -- Tsitsa River Catchment , Wetland plants -- Effect of grazing on
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/115936 , vital:34270
- Description: Wetlands are critical ecosystems that can provide services of great social, economic and environmental value to the society. Yet, in South Africa, hillslope seep wetlands are among the most threatened ecosystems due to human-induced activities and are disappearing rapidly. Further, despite the potential vulnerability of hillslope seep wetland to disturbances, and their criticality in relation to all year round provision of forage for livestock grazing, they are among the most poorly studied wetland systems. Using a social-ecological system framing, and drawing on a range of ecological and social sciences methods, this study shed light on ways in which an integrative approach can contribute to sustainable utilisation of hillslope seep wetlands in the Tsitsa River catchment in the Eastern Cape province of South Africa. The study specific objectives were to i) evaluate the performance of Floristic Quality Assessment Index (FQAI), WET-Health and Floristic Assessment Quotient for Wetlands Index (FQAWet) indices for assessing hillslope seep wetlands ecological health ii) develop a trait-based approach for assessing the potential resilience and vulnerability of hillslope seep wetland plant species to disturbances, iii) assess the role of hillslope seep wetlands in the local communities in relation to livestock, and explore understanding of local people about the value of hillslope seep wetlands, iv) demonstrate collaborative insights emerging from an integrative social-ecological system research process to inform sustainable management of hillslope seep wetlands. A total of 11 hillslope seep wetlands were visually classified based on the level of erosion. Plant species composition within the wetlands was determined along a 100 m line transects across the hillslope wetland sites. 5 quadrats of 0.2m2 were also placed along transect for vegetation collection and cover. The plant species collected were used to calculate (FQAI) and FQAWet scores to evaluate the condition of hillslope seep wetlands. The degree of human disturbances was assessed using the Anthropogenic Activity Index (AAI), an index for qualitatively assessing the degree of human disturbance based on visual inspection of a wetland site. Factors represented in the AAI, include: (i) surrounding land use intensity; (ii) soil disturbance; (iii) hydrological alteration; (iv) habitat alteration within wetland; (v) vegetation community quality. The vegetation samples were collected in summer 2016 and winter 2017. All assessed indices were regressed against AAI to evaluate their performances. All assessed indices FQAI, FQAWet and WET-Health showed that hillslope seep wetland were impacted by human activities. FQAIall and WET-Health showed the strongest response to AAI in winter, while FQAIdom and FAQWet showed a weak response to AAI in all seasons. Overall, the findings of this study suggest that FQAIall and WET-Health are potentially better tools for assessing the biological conditions of hillslope seep wetland in South Africa. A novel trait-based approach was developed using seven plant traits and 27 trait attributes. Based on the developed approach, plant species were grouped into three potentially vulnerable groups in relation to grazing pressure. It was then predicted that species belonging to the highly vulnerable group would be less dominant at the highly disturbed sites, as well as in the winter season when grazing pressure is at its highest. The result corresponds largely with the seasonal predictions; however this was not the case for sites. The approach developed in this study worked and it was useful for predicting the potential responses of plant species in hillslope seep wetlands to grazing pressure. The success of the approach seasonally could be attributed to the careful selection of the traits, reflecting the mechanistic relationship between the grazing mode of stress on vegetation and trait-mediated biotic response. However, this still need to be refined using accurate vegetation cover methods that might have had impact on the lack of correspondence within sites. The results of the present study revealed that communities largely perceive hillslope seep wetlands as important ecosystems for their livelihoods. They recognise that the importance stems from services provided by the wetlands, particularly for livestock grazing during the dry season. Although hillslope seep wetlands are viewed as important ecosystems for livelihoods, the communities also perceive these wetlands as highly eroded ecosystems. Community members indicate willingness to strengthen local natural resource governance systems, which could lead to better management of hillslope seep wetlands. A range of protective strategies for hillslope seep were suggested by community members, including fencing, active herding and rotational grazing. The study suggests that active involvement of local communities is critical to the successful management of natural resources. The study highlights the need to consider the role of local people as influential components within social-ecological systems in order to promote effective management and conservation interventions of hillslope seep wetlands. Overall, the study highlights the criticality of an integrative social-ecological system approach for holistic management of hillslope seep wetlands within the studied catchment.
- Full Text:
- Date Issued: 2019
- Authors: Libala, Notiswa
- Date: 2019
- Subjects: Wetland management -- South Africa -- Eastern Cape , Wetland ecology -- South Africa -- Eastern Cape , Wetland conservation -- South Africa -- Eastern Cape , Slopes (Physical geography) -- South Africa -- Eastern Cape , Vegetation management -- South Africa -- Tsitsa River Catchment , Land degradation -- Control -- South Africa -- Tsitsa River Catchment , Grazing -- Environmental aspects -- South Africa -- Tsitsa River Catchment , Ecosystem management -- South Africa -- Tsitsa River Catchment , Wetland plants -- Effect of grazing on
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/115936 , vital:34270
- Description: Wetlands are critical ecosystems that can provide services of great social, economic and environmental value to the society. Yet, in South Africa, hillslope seep wetlands are among the most threatened ecosystems due to human-induced activities and are disappearing rapidly. Further, despite the potential vulnerability of hillslope seep wetland to disturbances, and their criticality in relation to all year round provision of forage for livestock grazing, they are among the most poorly studied wetland systems. Using a social-ecological system framing, and drawing on a range of ecological and social sciences methods, this study shed light on ways in which an integrative approach can contribute to sustainable utilisation of hillslope seep wetlands in the Tsitsa River catchment in the Eastern Cape province of South Africa. The study specific objectives were to i) evaluate the performance of Floristic Quality Assessment Index (FQAI), WET-Health and Floristic Assessment Quotient for Wetlands Index (FQAWet) indices for assessing hillslope seep wetlands ecological health ii) develop a trait-based approach for assessing the potential resilience and vulnerability of hillslope seep wetland plant species to disturbances, iii) assess the role of hillslope seep wetlands in the local communities in relation to livestock, and explore understanding of local people about the value of hillslope seep wetlands, iv) demonstrate collaborative insights emerging from an integrative social-ecological system research process to inform sustainable management of hillslope seep wetlands. A total of 11 hillslope seep wetlands were visually classified based on the level of erosion. Plant species composition within the wetlands was determined along a 100 m line transects across the hillslope wetland sites. 5 quadrats of 0.2m2 were also placed along transect for vegetation collection and cover. The plant species collected were used to calculate (FQAI) and FQAWet scores to evaluate the condition of hillslope seep wetlands. The degree of human disturbances was assessed using the Anthropogenic Activity Index (AAI), an index for qualitatively assessing the degree of human disturbance based on visual inspection of a wetland site. Factors represented in the AAI, include: (i) surrounding land use intensity; (ii) soil disturbance; (iii) hydrological alteration; (iv) habitat alteration within wetland; (v) vegetation community quality. The vegetation samples were collected in summer 2016 and winter 2017. All assessed indices were regressed against AAI to evaluate their performances. All assessed indices FQAI, FQAWet and WET-Health showed that hillslope seep wetland were impacted by human activities. FQAIall and WET-Health showed the strongest response to AAI in winter, while FQAIdom and FAQWet showed a weak response to AAI in all seasons. Overall, the findings of this study suggest that FQAIall and WET-Health are potentially better tools for assessing the biological conditions of hillslope seep wetland in South Africa. A novel trait-based approach was developed using seven plant traits and 27 trait attributes. Based on the developed approach, plant species were grouped into three potentially vulnerable groups in relation to grazing pressure. It was then predicted that species belonging to the highly vulnerable group would be less dominant at the highly disturbed sites, as well as in the winter season when grazing pressure is at its highest. The result corresponds largely with the seasonal predictions; however this was not the case for sites. The approach developed in this study worked and it was useful for predicting the potential responses of plant species in hillslope seep wetlands to grazing pressure. The success of the approach seasonally could be attributed to the careful selection of the traits, reflecting the mechanistic relationship between the grazing mode of stress on vegetation and trait-mediated biotic response. However, this still need to be refined using accurate vegetation cover methods that might have had impact on the lack of correspondence within sites. The results of the present study revealed that communities largely perceive hillslope seep wetlands as important ecosystems for their livelihoods. They recognise that the importance stems from services provided by the wetlands, particularly for livestock grazing during the dry season. Although hillslope seep wetlands are viewed as important ecosystems for livelihoods, the communities also perceive these wetlands as highly eroded ecosystems. Community members indicate willingness to strengthen local natural resource governance systems, which could lead to better management of hillslope seep wetlands. A range of protective strategies for hillslope seep were suggested by community members, including fencing, active herding and rotational grazing. The study suggests that active involvement of local communities is critical to the successful management of natural resources. The study highlights the need to consider the role of local people as influential components within social-ecological systems in order to promote effective management and conservation interventions of hillslope seep wetlands. Overall, the study highlights the criticality of an integrative social-ecological system approach for holistic management of hillslope seep wetlands within the studied catchment.
- Full Text:
- Date Issued: 2019
Aiding the education agenda? the role of non-governmental organisations in learner performance and retention in Joza, Grahamstown, South Africa
- Authors: Nomsenge, Sinazo Onela
- Date: 2019
- Subjects: Non-governmental organizations , Non-governmental organizations -- South Africa -- Makhanda , Black people -- Education -- South Africa -- Makhanda , Education -- South Africa -- Makhanda , Joza Youth Hub (Makhanda) , Upstart Youth Development Project (Makhanda) , Village Scribe Association (Makhanda) , Ikamba Youth (Makhanda) , Access Music Project(Makhanda)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/76468 , vital:30568
- Description: This thesis describes the network of complexities that characterise the world and work of Non-Governmental Organisations (NGOs). It examines the ways in which organisations navigate different internal, sectoral and contextual intricacies while operating under the command of their chosen developmental mandates. This description is drawn from a sociological analysis of the internal workings of education NGOs, their external affiliations as well as the negotiations which underpin their operations and survival. Collectively, the careful illustration of these underpinnings outlines both the role that NGOs play in the performance and retention of learners in the Grahamstown-east township of Joza and also their position in the town’s basic education sector. Private and non-governmental interveners have, particularly from the closing decades of the 20th century, been conceptually and operationally deployed as panaceas of the socio-economic scarcities which continue to pervade much of the ‘developing’ world. Their involvement in the socio-economic missions of populations living in the Global South has grown both laterally and in the depth of how development is understood and defined, carried out and also measured. NGOs, as widely acclaimed institutional arms of global development imperatives, therefore assume prominent positions in framing policy and implementation models, prescribing performance benchmarks and pronouncing non-compliance. Likewise, education NGOs have obtained normative prescription status in global education policy and practice largely on the back of the Education for All (EFA) objectives, the Millennium Development Goals (MDGs) and the neo-liberal logics which have championed state retreat in favour of private sector ascendance in globalised development. This, in conjunction with the persistent struggles of educational transformation in the Global South, has given NGOs little trouble in legitimising their prominent presence in education and other sectors of socio-economic relief in these parts of the world. Little illusion remains however, in many commentary circles, of the role of NGOs in advancing development ideals, the honesty of their altruistic intents, their ideological leanings as well as their efficacy in carrying out their mandates. As such, the logics which have been used to dethrone developing state structures in order to expand the space for private intervention along with the prevailing and deepening markers of educational underperformance, have been central features of the criticisms levelled against NGOs. This thesis intervenes in these ongoing reflections by describing the role of NGOs in educational outcomes, particularly learner performance and retention in Joza. This analysis demonstrates the organisational, sector-level and broader community forces which influence not only the form which non-state interventions take on and the daily preoccupations of their carriers but broadly, the position they occupy in the town’s overall educational profile. By way of locating NGOs within Grahamstown’s educational landscape, this thesis first demonstrates, the conflicted nature of NGO operations from an international, sectoral, national, local and organisational level. The discussion then illustrates how the preoccupations of NGOs are scattered between the different communities which they occupy within these levels. Their reliance on these players demands that organisations be tactical in guarding both their survival and, at times, the conflicting allegiances which grant them different forms of legitimacy. Internal struggles which characterise this imbalance of forces results in a trade-off which often favours organisational preservation mechanisms over systemic educational overhaul. As such, while non-state interveners can be lauded for extending educational support to those who would otherwise not have such, the gains of NGO intervention are often absorbed by internal urgencies for organisational legitimacy and preservation. This, in a context which possesses a unique set of socio-economic and educational deficits that require, at the very least, radical and unbridled mediation, means that pre-existing inequalities in educational inputs and outcomes along with the resultant inequities in youth socio-economic prospects, can find refuge in the very sector whose support and intervention is sought out and justified for such. This thesis lays out the nuances of these tensions and contradictions and offers this case as a point of reference for further considerations of the persistent markers of underperformance which characterise developing communities that enjoy high concentrations of non-state educational intervention.
- Full Text:
- Date Issued: 2019
- Authors: Nomsenge, Sinazo Onela
- Date: 2019
- Subjects: Non-governmental organizations , Non-governmental organizations -- South Africa -- Makhanda , Black people -- Education -- South Africa -- Makhanda , Education -- South Africa -- Makhanda , Joza Youth Hub (Makhanda) , Upstart Youth Development Project (Makhanda) , Village Scribe Association (Makhanda) , Ikamba Youth (Makhanda) , Access Music Project(Makhanda)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/76468 , vital:30568
- Description: This thesis describes the network of complexities that characterise the world and work of Non-Governmental Organisations (NGOs). It examines the ways in which organisations navigate different internal, sectoral and contextual intricacies while operating under the command of their chosen developmental mandates. This description is drawn from a sociological analysis of the internal workings of education NGOs, their external affiliations as well as the negotiations which underpin their operations and survival. Collectively, the careful illustration of these underpinnings outlines both the role that NGOs play in the performance and retention of learners in the Grahamstown-east township of Joza and also their position in the town’s basic education sector. Private and non-governmental interveners have, particularly from the closing decades of the 20th century, been conceptually and operationally deployed as panaceas of the socio-economic scarcities which continue to pervade much of the ‘developing’ world. Their involvement in the socio-economic missions of populations living in the Global South has grown both laterally and in the depth of how development is understood and defined, carried out and also measured. NGOs, as widely acclaimed institutional arms of global development imperatives, therefore assume prominent positions in framing policy and implementation models, prescribing performance benchmarks and pronouncing non-compliance. Likewise, education NGOs have obtained normative prescription status in global education policy and practice largely on the back of the Education for All (EFA) objectives, the Millennium Development Goals (MDGs) and the neo-liberal logics which have championed state retreat in favour of private sector ascendance in globalised development. This, in conjunction with the persistent struggles of educational transformation in the Global South, has given NGOs little trouble in legitimising their prominent presence in education and other sectors of socio-economic relief in these parts of the world. Little illusion remains however, in many commentary circles, of the role of NGOs in advancing development ideals, the honesty of their altruistic intents, their ideological leanings as well as their efficacy in carrying out their mandates. As such, the logics which have been used to dethrone developing state structures in order to expand the space for private intervention along with the prevailing and deepening markers of educational underperformance, have been central features of the criticisms levelled against NGOs. This thesis intervenes in these ongoing reflections by describing the role of NGOs in educational outcomes, particularly learner performance and retention in Joza. This analysis demonstrates the organisational, sector-level and broader community forces which influence not only the form which non-state interventions take on and the daily preoccupations of their carriers but broadly, the position they occupy in the town’s overall educational profile. By way of locating NGOs within Grahamstown’s educational landscape, this thesis first demonstrates, the conflicted nature of NGO operations from an international, sectoral, national, local and organisational level. The discussion then illustrates how the preoccupations of NGOs are scattered between the different communities which they occupy within these levels. Their reliance on these players demands that organisations be tactical in guarding both their survival and, at times, the conflicting allegiances which grant them different forms of legitimacy. Internal struggles which characterise this imbalance of forces results in a trade-off which often favours organisational preservation mechanisms over systemic educational overhaul. As such, while non-state interveners can be lauded for extending educational support to those who would otherwise not have such, the gains of NGO intervention are often absorbed by internal urgencies for organisational legitimacy and preservation. This, in a context which possesses a unique set of socio-economic and educational deficits that require, at the very least, radical and unbridled mediation, means that pre-existing inequalities in educational inputs and outcomes along with the resultant inequities in youth socio-economic prospects, can find refuge in the very sector whose support and intervention is sought out and justified for such. This thesis lays out the nuances of these tensions and contradictions and offers this case as a point of reference for further considerations of the persistent markers of underperformance which characterise developing communities that enjoy high concentrations of non-state educational intervention.
- Full Text:
- Date Issued: 2019
The morphological complexity of L1 Arabic-speaking children
- Authors: Issa, Iyad
- Date: 2019
- Subjects: Cognition in children , Reading , Arabic language -- Orthography and spelling , Arabic language -- Orthography and spelling -- Study and teaching , Arabic language -- Study and teaching , Arabic language -- Phonetics
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/92831 , vital:30754
- Description: Spelling poses a challenge to Arabic-speaking learners due to the complexity of the morphological and orthographic system in Arabic. Arabic morphology has been argued to play a critical role in spelling since its morphological operations are built on a system consisting of a root that is interlocking into different patterns of vowels to form different categories of words. In addition, Arabic orthography is considered to be loyal to the morphographic principle (Ravid, 2012), where morphemes correspond to graphic representation regardless of the pronunciation, especially in the non-vowelized texts. This study made a detailed classification of spelling errors in a word dictation task, based on morphological structures, undertaken by 107 Typically-developing learners (TD) and learners with learning disabiities (LD) attending the same schools. All participants ranged in age from 7 years, 3 months to 15 years, 2 months (grades 2 to 8). The spelling task was made up of 400 common words representing all morphological forms in different conjugations and grammatical classes. The results indicated that learners made three types of errors: errors with respect to the root, errors with respect to the word pattern, and errors with respect to both the root and the word pattern. The results also showed that TD and LD learners follow a similar pattern of complexity even though the LD group produced more errors than the TD group. The results revealed that MA and PA exhibited significant positive regression (b= 9.398, 16.106 respectively) with spelling, indicating that learners with higher scores in PA and MA have higher scores in spelling. The results argued for the crucial contribution that morphological awareness makes towards the general spelling abilities among learners and provide additional evidence for the nonlinear growth of morphological knowedge in spelling. In addition, spelling errors suggested that the spelling process goes in a hierarchical way where words can be accessed and processed either according to the root or according to the stem. Intact verbs are processed according to their root and word pattern. Some weak verb forms, whose radicals undergo modifications, are processed according to their stem, while those whose radicals are fully represented in the spoken word, are processed according to their root and word patterns. Therefore, roots or stems are firstly accessed and attached to basic word patterns (the grapheme without diacritics and affixes). Thereafter, prefixes and, then, suffixes are attached to the word pattern and, finally, diacritics are accessed and attached to the word pattern.
- Full Text:
- Date Issued: 2019
- Authors: Issa, Iyad
- Date: 2019
- Subjects: Cognition in children , Reading , Arabic language -- Orthography and spelling , Arabic language -- Orthography and spelling -- Study and teaching , Arabic language -- Study and teaching , Arabic language -- Phonetics
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/92831 , vital:30754
- Description: Spelling poses a challenge to Arabic-speaking learners due to the complexity of the morphological and orthographic system in Arabic. Arabic morphology has been argued to play a critical role in spelling since its morphological operations are built on a system consisting of a root that is interlocking into different patterns of vowels to form different categories of words. In addition, Arabic orthography is considered to be loyal to the morphographic principle (Ravid, 2012), where morphemes correspond to graphic representation regardless of the pronunciation, especially in the non-vowelized texts. This study made a detailed classification of spelling errors in a word dictation task, based on morphological structures, undertaken by 107 Typically-developing learners (TD) and learners with learning disabiities (LD) attending the same schools. All participants ranged in age from 7 years, 3 months to 15 years, 2 months (grades 2 to 8). The spelling task was made up of 400 common words representing all morphological forms in different conjugations and grammatical classes. The results indicated that learners made three types of errors: errors with respect to the root, errors with respect to the word pattern, and errors with respect to both the root and the word pattern. The results also showed that TD and LD learners follow a similar pattern of complexity even though the LD group produced more errors than the TD group. The results revealed that MA and PA exhibited significant positive regression (b= 9.398, 16.106 respectively) with spelling, indicating that learners with higher scores in PA and MA have higher scores in spelling. The results argued for the crucial contribution that morphological awareness makes towards the general spelling abilities among learners and provide additional evidence for the nonlinear growth of morphological knowedge in spelling. In addition, spelling errors suggested that the spelling process goes in a hierarchical way where words can be accessed and processed either according to the root or according to the stem. Intact verbs are processed according to their root and word pattern. Some weak verb forms, whose radicals undergo modifications, are processed according to their stem, while those whose radicals are fully represented in the spoken word, are processed according to their root and word patterns. Therefore, roots or stems are firstly accessed and attached to basic word patterns (the grapheme without diacritics and affixes). Thereafter, prefixes and, then, suffixes are attached to the word pattern and, finally, diacritics are accessed and attached to the word pattern.
- Full Text:
- Date Issued: 2019
Synthesis, characterisation and biological evaluation of novel anti-infective compounds bearing ferrocene, arylpyrrole, thiazolidinedione, quinoline and triazole moieties
- Authors: Oderinlo, Ogunyemi Olajide
- Date: 2019
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/114714 , vital:34016 , 10.21504/10962/114714
- Description: Expected release date-April 2022
- Full Text: false
- Date Issued: 2019
- Authors: Oderinlo, Ogunyemi Olajide
- Date: 2019
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/114714 , vital:34016 , 10.21504/10962/114714
- Description: Expected release date-April 2022
- Full Text: false
- Date Issued: 2019
Determining the hydrological functioning of the palmiet wetlands in the Eastern and Western Cape South Africa
- Authors: Smith, Caitlin
- Date: 2019
- Subjects: Wetlands -- South Africa -- Eastern Cape , Wetland ecology -- South Africa -- Eastern Cape , Wetland management -- South Africa -- Eastern Cape , Prioniaceae -- South Africa -- Eastern Cape , Prionium serratum -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/95956 , vital:31218
- Description: Wetlands provide a range of supporting, regulating and provisioning ecosystem services, including hydrological benefits such as flood attenuation and sustaining base flows. Despite their value, wetlands are one of the most vulnerable ecosystems in South Africa. Palmiet wetlands in the Eastern and Western Cape are particularly threatened. Palmiet (Prionium serratum) is a robust perennial plant that is endemic to wetlands and rivers located in the sandstones and quartzites of the Table Mountain Group (TMG), in the Eastern and Western Cape as well as the Natal Group sandstones in KwaZulu-Natal. Palmiet is described as an ecosystem engineer because of its ability to alter its environment and create large valley-bottom wetlands. The Krom River is an important water source for the city of Port Elizabeth and there has been a decline in palmiet wetlands along the Krom River as a result of alien vegetation invasion, agricultural activity, and gully erosion. Working for Water has been clearing alien vegetation and Working for Wetlands has been installing rehabilitation structures in the Krom River catchment for a number of years. There are, however, serious knowledge gaps in the understanding of palmiet wetland structure and function, particularly in respect of the hydrological functioning of these wetland systems. The aim of this study was to investigate the hydrology (surface and groundwater) behind these wetland systems. The investigation focussed on small-scale dynamics of the palmiet wetland system in order to increase general understanding of the surface water and groundwater processes of these wetland systems. Field work was concentrated on the Kompanjiesdrif and Krugersland palmiet wetlands in the upper K90A Krom River catchment. The investigation involved the installation of piezometers, water quality and stable isotope sampling and analysis, an Electrical Resistivity Tomography survey, and hydrological and mixing cell modelling. The results of the investigation indicate that the hydrological functioning of palmiet wetlands is closely linked with high sub-surface discharges typically associated with TMG aquifers. It is proposed that the palmiet wetlands are sustained by significant amounts of sub-surface water (both groundwater and interflow) moving through preferential flow paths in the alluvial fans and tributaries, which are in turn sustained by groundwater discharge from the surrounding sandstones and quartzites of the Nardouw Sub-group and Peninsula Formation. The palmiet wetlands clearly retain a significant amount of water, leading to the maintenance of prolonged flows, and a larger baseflow. However, it is hypothesised that the occurrence of palmiet as the dominant species in these wetlands is due to the sustained low flows related to catchment geology and high hydrological connectivity between the catchment and the wetland that is enabled by flow paths that allow the free flow of water from the catchment to the wetland. It is further proposed that palmiet is possibly more reliant on a consistent water supply for its existence and survival than it is on acidic nutrient-poor water and soils as stated by other authors.
- Full Text:
- Date Issued: 2019
- Authors: Smith, Caitlin
- Date: 2019
- Subjects: Wetlands -- South Africa -- Eastern Cape , Wetland ecology -- South Africa -- Eastern Cape , Wetland management -- South Africa -- Eastern Cape , Prioniaceae -- South Africa -- Eastern Cape , Prionium serratum -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/95956 , vital:31218
- Description: Wetlands provide a range of supporting, regulating and provisioning ecosystem services, including hydrological benefits such as flood attenuation and sustaining base flows. Despite their value, wetlands are one of the most vulnerable ecosystems in South Africa. Palmiet wetlands in the Eastern and Western Cape are particularly threatened. Palmiet (Prionium serratum) is a robust perennial plant that is endemic to wetlands and rivers located in the sandstones and quartzites of the Table Mountain Group (TMG), in the Eastern and Western Cape as well as the Natal Group sandstones in KwaZulu-Natal. Palmiet is described as an ecosystem engineer because of its ability to alter its environment and create large valley-bottom wetlands. The Krom River is an important water source for the city of Port Elizabeth and there has been a decline in palmiet wetlands along the Krom River as a result of alien vegetation invasion, agricultural activity, and gully erosion. Working for Water has been clearing alien vegetation and Working for Wetlands has been installing rehabilitation structures in the Krom River catchment for a number of years. There are, however, serious knowledge gaps in the understanding of palmiet wetland structure and function, particularly in respect of the hydrological functioning of these wetland systems. The aim of this study was to investigate the hydrology (surface and groundwater) behind these wetland systems. The investigation focussed on small-scale dynamics of the palmiet wetland system in order to increase general understanding of the surface water and groundwater processes of these wetland systems. Field work was concentrated on the Kompanjiesdrif and Krugersland palmiet wetlands in the upper K90A Krom River catchment. The investigation involved the installation of piezometers, water quality and stable isotope sampling and analysis, an Electrical Resistivity Tomography survey, and hydrological and mixing cell modelling. The results of the investigation indicate that the hydrological functioning of palmiet wetlands is closely linked with high sub-surface discharges typically associated with TMG aquifers. It is proposed that the palmiet wetlands are sustained by significant amounts of sub-surface water (both groundwater and interflow) moving through preferential flow paths in the alluvial fans and tributaries, which are in turn sustained by groundwater discharge from the surrounding sandstones and quartzites of the Nardouw Sub-group and Peninsula Formation. The palmiet wetlands clearly retain a significant amount of water, leading to the maintenance of prolonged flows, and a larger baseflow. However, it is hypothesised that the occurrence of palmiet as the dominant species in these wetlands is due to the sustained low flows related to catchment geology and high hydrological connectivity between the catchment and the wetland that is enabled by flow paths that allow the free flow of water from the catchment to the wetland. It is further proposed that palmiet is possibly more reliant on a consistent water supply for its existence and survival than it is on acidic nutrient-poor water and soils as stated by other authors.
- Full Text:
- Date Issued: 2019
Narratives and home: remembering an almost forgotten walk
- Bezuidenhout, Natasha Belinda
- Authors: Bezuidenhout, Natasha Belinda
- Date: 2019
- Subjects: Home in literature , Home in art , Artists -- South Africa
- Language: English
- Type: text , Thesis , Masters , MFA
- Identifier: http://hdl.handle.net/10962/115039 , vital:34072
- Description: The title of my exhibition Bittersoet alludes to the self-exploratory nature of my practice, as I interrogate the personal memories associated with objects that characterise the relationship between myself and my mother (mamma). This supporting document, Narratives and Home: Remembering an almost forgotten walk, considers the key conceptual concerns informing my practice. In this mini-thesis, I address the question: ‘What is a home?’. Drawing from my own Fine Art Practice, I explore how home can be examined as a product of the imagination, rather than only as a physical place. I consider how ‘home’ is constructed as the primary objective within an ideological framework defined by history, memory and narrative. Engaging beyond the idea of ‘home’ as a fixed structure or place, I examine the idea of ‘home’ as something fluid that is negotiated and defined by the interaction between objects and language. It is concerned with dialectics of memory and narrative as they pertain directly to an experience of both searching for and reimagining home through metaphorical representations. In particular, I explore how home can be seen as equally familiar and unfamiliar, existing in-between, always changing, never fixed, rather in a constant state of flux. The concept of home is addressed in a dialogical process by using Afrikaans as my mother tongue, I narrate informal conversations between myself and my mother. These conversations transform and expand into hybrid words, memories and narratives to form a layered continuous dialogue between my practice and research. This notion relates to exploring oneself and the ‘fictions’ of the past. The self being fundamental to the individual comprehension of both ‘place’ and ‘space’.
- Full Text:
- Date Issued: 2019
- Authors: Bezuidenhout, Natasha Belinda
- Date: 2019
- Subjects: Home in literature , Home in art , Artists -- South Africa
- Language: English
- Type: text , Thesis , Masters , MFA
- Identifier: http://hdl.handle.net/10962/115039 , vital:34072
- Description: The title of my exhibition Bittersoet alludes to the self-exploratory nature of my practice, as I interrogate the personal memories associated with objects that characterise the relationship between myself and my mother (mamma). This supporting document, Narratives and Home: Remembering an almost forgotten walk, considers the key conceptual concerns informing my practice. In this mini-thesis, I address the question: ‘What is a home?’. Drawing from my own Fine Art Practice, I explore how home can be examined as a product of the imagination, rather than only as a physical place. I consider how ‘home’ is constructed as the primary objective within an ideological framework defined by history, memory and narrative. Engaging beyond the idea of ‘home’ as a fixed structure or place, I examine the idea of ‘home’ as something fluid that is negotiated and defined by the interaction between objects and language. It is concerned with dialectics of memory and narrative as they pertain directly to an experience of both searching for and reimagining home through metaphorical representations. In particular, I explore how home can be seen as equally familiar and unfamiliar, existing in-between, always changing, never fixed, rather in a constant state of flux. The concept of home is addressed in a dialogical process by using Afrikaans as my mother tongue, I narrate informal conversations between myself and my mother. These conversations transform and expand into hybrid words, memories and narratives to form a layered continuous dialogue between my practice and research. This notion relates to exploring oneself and the ‘fictions’ of the past. The self being fundamental to the individual comprehension of both ‘place’ and ‘space’.
- Full Text:
- Date Issued: 2019
An analysis of the roles and functions of teachers’ gestures as visualisation tools in the teaching of mathematics at the junior primary phase (Grades 0 –3)
- Authors: Namakalu, Dietlinde Nelao
- Date: 2019
- Subjects: Gesture , Gesture in mathematics education , Visual learning , Visualization , Education, Elementary -- Namibia
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/96713 , vital:31311
- Description: There is ample evidence in the literature that gestures are important hand and bodily actions that play vital roles during interactions between learners and teachers in the mathematics classroom. These gestures enable both the teacher and the learner to visualise mathematical concepts and ideas. The aim of this Namibian interpretive case study, located in enactivist theory, was to analyse the types of gestures that three selected teachers at the junior primary phase (Grades 0–3) used in their teaching of mathematics. The study also interrogated the roles that these gestures played in the teaching process of the three teachers. As I intended to analyse the types of gestures the selected teachers used and understand their views and perceptions of the roles and functions their gestures played as visualisation tools in the teaching of mathematics, I observed ten lessons of each teacher and video recorded them. I then interviewed them (one-on-one stimulus-recall interviews) and interpreted the gestures they used and utterances they made about using these gestures. The types of gestures that the participating teachers used are classified according to McNeill‘s (1992) framework, namely pointing (deictic) gestures, iconic (illustrators) gestures, metaphoric gestures, beat (motor) gestures and symbolic (emblems) gestures. The study found that the participating teachers incorporated a variety of gestures into their lessons and used them strategically. According to the teachers, gestures made the lessons interesting and encouraged active participation of the learners in the lessons. The teachers also revealed that gestures assist in explaining mathematical concepts and thus affected the learners‘ understanding positively. This study strongly suggests that the usefulness and efficacy of employing gestures as visualisation tools in mathematics education should be strategically harnessed.
- Full Text:
- Date Issued: 2019
- Authors: Namakalu, Dietlinde Nelao
- Date: 2019
- Subjects: Gesture , Gesture in mathematics education , Visual learning , Visualization , Education, Elementary -- Namibia
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/96713 , vital:31311
- Description: There is ample evidence in the literature that gestures are important hand and bodily actions that play vital roles during interactions between learners and teachers in the mathematics classroom. These gestures enable both the teacher and the learner to visualise mathematical concepts and ideas. The aim of this Namibian interpretive case study, located in enactivist theory, was to analyse the types of gestures that three selected teachers at the junior primary phase (Grades 0–3) used in their teaching of mathematics. The study also interrogated the roles that these gestures played in the teaching process of the three teachers. As I intended to analyse the types of gestures the selected teachers used and understand their views and perceptions of the roles and functions their gestures played as visualisation tools in the teaching of mathematics, I observed ten lessons of each teacher and video recorded them. I then interviewed them (one-on-one stimulus-recall interviews) and interpreted the gestures they used and utterances they made about using these gestures. The types of gestures that the participating teachers used are classified according to McNeill‘s (1992) framework, namely pointing (deictic) gestures, iconic (illustrators) gestures, metaphoric gestures, beat (motor) gestures and symbolic (emblems) gestures. The study found that the participating teachers incorporated a variety of gestures into their lessons and used them strategically. According to the teachers, gestures made the lessons interesting and encouraged active participation of the learners in the lessons. The teachers also revealed that gestures assist in explaining mathematical concepts and thus affected the learners‘ understanding positively. This study strongly suggests that the usefulness and efficacy of employing gestures as visualisation tools in mathematics education should be strategically harnessed.
- Full Text:
- Date Issued: 2019
Investigating assay formats for screening malaria Hsp90-Hop interaction inhibitors
- Authors: Derry, Leigh-Anne Tracy Kim
- Date: 2019
- Subjects: Antimalarials , Heat shock proteins , Drug interactions , Drug resistance , Plasmodium falciparum , High throughput screening (Drug development) , Bioluminescence resonance energy transfer (BRET) , Fluorescence resonance energy transfer (FRET)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63345 , vital:28395
- Description: Although significant gains have been made in the combat against malaria in the last decade, the persistent threat of drug and insecticide resistance continues to motivate the search for new classes of antimalarial drug compounds and targets. Due to their predominance in cellular reactions, protein-protein interactions (P-PIs) are emerging as a promising general target class for therapeutic development. The P-PI which is the focus of this project is the interaction between the chaperone heat shock protein 90 (Hsp90) and its co-chaperone Hsp70/Hsp90 organising protein (Hop). Hop binds to Hsp70 and Hsp90 and facilitates the transfer of client proteins (proteins undergoing folding) from the former to the latter and also regulates nucleotide exchange on Hsp90. Due to its role in correcting protein misfolding during cell stress, Hsp90 is being pursued as a cancer drug target and compounds that inhibit its ATPase activity have entered clinical trials. However, it has been proposed that inhibiting the interaction between Hsp90 and Hop may be alternative approach for inhibiting Hsp90 function for cancer therapy. The malaria parasite Plasmodium falciparum experiences temperature fluctuations during vector-host transitions and febrile episodes and cell stress due to rapid growth and immune responses. Hence, it also depends on chaperones, including PfHsp90, to maintain protein functionality and pathogenesis, demonstrated inter alia by the sensitivity of parasites to Hsp90 inhibitors. In addition, PfHsp90 exists as a complex with the malarial Hop homologue, PfHop, in parasite lysates. Consequently, the purpose of this study was to explore P-PI assay formats that can confirm the interaction of PfHsp90 and PfHop and can be used to identify inhibitors of the interaction, preferably in a medium- to high-throughput screening mode. As a first approach, cell-based bioluminescence and fluorescence resonance energy transfer (BRET and FRET) assays were performed in HeLa cells. To facilitate this, expression plasmid constructs containing coding sequences of P. falciparum and mammalian Hsp90 and Hop and their interacting domains (Hsp90 C-domain and Hop TPR2A domain) fused to the BRET and FRET reporter proteins – yellow fluorescent protein (YFP), cyan fluorescent protein (CFP) and Renilla luciferase (Rluc) - were prepared and used for HeLa cell transient transfections. The FRET assay produced positive interaction signals for the full-length P. falciparum and mammalian Hsp90-Hop interactions. However, C-domain-TPR2A domain interactions were not detected, no interactions could be demonstrated with the BRET assay and western blotting experiments failed to detect expression of all the interaction partners in transiently transfected HeLa cells. Consequently, an alternative in vitro FRET assay format using recombinant proteins was investigated. Expression constructs for the P. falciparum and mammalian C-domains and TPR2A domains fused respectively to YFP and CFP were prepared and the corresponding fusion proteins expressed and purified from E. coli. No interaction was found with the mammalian interaction partners, but interaction of the P. falciparum C-domain and TPR2A domain was consistently detected with a robust Z’ factor value of 0.54. A peptide corresponding to the PfTPR2A domain sequence primarily responsible for Hsp90 binding (based on a human TPR2A peptide described by Horibe et al., 2011) was designed and showed dose-dependent inhibition of the interaction, with 53.7% inhibition at 100 μM. The components of the assay are limited to the purified recombinant proteins, requires minimal liquid steps and may thus be a useful primary screening format for identifying inhibitors of P. falciparum Hsp90-Hop interaction.
- Full Text:
- Date Issued: 2019
- Authors: Derry, Leigh-Anne Tracy Kim
- Date: 2019
- Subjects: Antimalarials , Heat shock proteins , Drug interactions , Drug resistance , Plasmodium falciparum , High throughput screening (Drug development) , Bioluminescence resonance energy transfer (BRET) , Fluorescence resonance energy transfer (FRET)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63345 , vital:28395
- Description: Although significant gains have been made in the combat against malaria in the last decade, the persistent threat of drug and insecticide resistance continues to motivate the search for new classes of antimalarial drug compounds and targets. Due to their predominance in cellular reactions, protein-protein interactions (P-PIs) are emerging as a promising general target class for therapeutic development. The P-PI which is the focus of this project is the interaction between the chaperone heat shock protein 90 (Hsp90) and its co-chaperone Hsp70/Hsp90 organising protein (Hop). Hop binds to Hsp70 and Hsp90 and facilitates the transfer of client proteins (proteins undergoing folding) from the former to the latter and also regulates nucleotide exchange on Hsp90. Due to its role in correcting protein misfolding during cell stress, Hsp90 is being pursued as a cancer drug target and compounds that inhibit its ATPase activity have entered clinical trials. However, it has been proposed that inhibiting the interaction between Hsp90 and Hop may be alternative approach for inhibiting Hsp90 function for cancer therapy. The malaria parasite Plasmodium falciparum experiences temperature fluctuations during vector-host transitions and febrile episodes and cell stress due to rapid growth and immune responses. Hence, it also depends on chaperones, including PfHsp90, to maintain protein functionality and pathogenesis, demonstrated inter alia by the sensitivity of parasites to Hsp90 inhibitors. In addition, PfHsp90 exists as a complex with the malarial Hop homologue, PfHop, in parasite lysates. Consequently, the purpose of this study was to explore P-PI assay formats that can confirm the interaction of PfHsp90 and PfHop and can be used to identify inhibitors of the interaction, preferably in a medium- to high-throughput screening mode. As a first approach, cell-based bioluminescence and fluorescence resonance energy transfer (BRET and FRET) assays were performed in HeLa cells. To facilitate this, expression plasmid constructs containing coding sequences of P. falciparum and mammalian Hsp90 and Hop and their interacting domains (Hsp90 C-domain and Hop TPR2A domain) fused to the BRET and FRET reporter proteins – yellow fluorescent protein (YFP), cyan fluorescent protein (CFP) and Renilla luciferase (Rluc) - were prepared and used for HeLa cell transient transfections. The FRET assay produced positive interaction signals for the full-length P. falciparum and mammalian Hsp90-Hop interactions. However, C-domain-TPR2A domain interactions were not detected, no interactions could be demonstrated with the BRET assay and western blotting experiments failed to detect expression of all the interaction partners in transiently transfected HeLa cells. Consequently, an alternative in vitro FRET assay format using recombinant proteins was investigated. Expression constructs for the P. falciparum and mammalian C-domains and TPR2A domains fused respectively to YFP and CFP were prepared and the corresponding fusion proteins expressed and purified from E. coli. No interaction was found with the mammalian interaction partners, but interaction of the P. falciparum C-domain and TPR2A domain was consistently detected with a robust Z’ factor value of 0.54. A peptide corresponding to the PfTPR2A domain sequence primarily responsible for Hsp90 binding (based on a human TPR2A peptide described by Horibe et al., 2011) was designed and showed dose-dependent inhibition of the interaction, with 53.7% inhibition at 100 μM. The components of the assay are limited to the purified recombinant proteins, requires minimal liquid steps and may thus be a useful primary screening format for identifying inhibitors of P. falciparum Hsp90-Hop interaction.
- Full Text:
- Date Issued: 2019
The impact of unanticipated news announcements by the US Federal Reserve On South African stock returns
- Authors: Sibanda, Lorna
- Date: 2019
- Subjects: Monetary policy -- United States , International finance , South Africa -- Foreign economic relations -- United States , United States -- Foreign economic relations -- South Africa , Banks of issue -- United States , Investments -- South Africa , Stocks -- Prices -- South Africa , Stocks -- Rate of return , Rate of return -- South Africa
- Language: English
- Type: text , Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10962/94703 , vital:31070
- Description: This thesis analyses whether monetary policy announcement shocks are transmitted across countries, with special emphasis on the impact of US Federal Reserve announcements on the South African stock market. Monetary policy is an important source of economic news and affects the risk perceptions of market participants. This study will improve the understanding of stock price determinants and possibly influence SA monetary policy in guarding against possible shocks originating from abroad. Using Federal Reserve Open Market Committee (FOMC) announcements over the period 2008 – 2014, the research studied changes in volatility of the South African FTSE/JSE All Share Index returns over this period. An event study and GARCH model approach was adopted to reach the goals of the analysis. The findings were a statistically insignificant connection between SA stock returns and both anticipated and unanticipated US Federal Reserve announcements. Over the sample period, each shock to SA stock returns persisted for approximately 4-5 months. Although SA stock return volatility demonstrated clustering behaviour (indicating sensitivity to economic shocks), the research could not find an obvious relationship between these spikes in volatility and US Federal Reserve announcements. It is concluded that South African stock returns do not change in response to unexpected US monetary policy announcements.
- Full Text:
- Date Issued: 2019
- Authors: Sibanda, Lorna
- Date: 2019
- Subjects: Monetary policy -- United States , International finance , South Africa -- Foreign economic relations -- United States , United States -- Foreign economic relations -- South Africa , Banks of issue -- United States , Investments -- South Africa , Stocks -- Prices -- South Africa , Stocks -- Rate of return , Rate of return -- South Africa
- Language: English
- Type: text , Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10962/94703 , vital:31070
- Description: This thesis analyses whether monetary policy announcement shocks are transmitted across countries, with special emphasis on the impact of US Federal Reserve announcements on the South African stock market. Monetary policy is an important source of economic news and affects the risk perceptions of market participants. This study will improve the understanding of stock price determinants and possibly influence SA monetary policy in guarding against possible shocks originating from abroad. Using Federal Reserve Open Market Committee (FOMC) announcements over the period 2008 – 2014, the research studied changes in volatility of the South African FTSE/JSE All Share Index returns over this period. An event study and GARCH model approach was adopted to reach the goals of the analysis. The findings were a statistically insignificant connection between SA stock returns and both anticipated and unanticipated US Federal Reserve announcements. Over the sample period, each shock to SA stock returns persisted for approximately 4-5 months. Although SA stock return volatility demonstrated clustering behaviour (indicating sensitivity to economic shocks), the research could not find an obvious relationship between these spikes in volatility and US Federal Reserve announcements. It is concluded that South African stock returns do not change in response to unexpected US monetary policy announcements.
- Full Text:
- Date Issued: 2019
The use of filter-feeding fish (Clarias gariepinus and Oreochromis mossambicus) to remove microalgae from brewery effluent treatment ponds
- Authors: Nombembe, Lwazi
- Date: 2019
- Subjects: Clarias gariepinus -- Food , Mozambique tilapia -- Food , Water -- Purification -- South Africa , Algae -- Biotechnology -- South Africa , Microalgae -- Biotechnology-- South Africa , Brewery waste -- South Africa , Fish culture -- South Africa
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/95745 , vital:31194
- Description: The removal of microalgae from high rate algal ponds (HRAP) in waste-water treatment systems remains a constraint to their use in effluent treatment systems. Conventional algae harvesting methods often have high energy demands, take up lots of space, are expensive to operate or are time consuming. The aim of the study was to determine if fish such as Clarias gariepinus and Oreochromis mossambicus, could be used to remove microalgae from waste-water treatment ponds (in the absence/presence of a flocculent in the former and in the absence or presence of pH moderation in the latter), and to investigate the subsequent influence of algae concentration on several water quality parameters. The age of Clarias gariepinus (3-12 months) had a positive relationship with the distance between gill rakers (98.27 to 163.34 μm; y=90.576+4.823*x: R²=0.549; F(1,18)=21.867; p<0.001) and these data suggested that these fish might be efficient at removing algae from HRAP effluent. However, this was not the case, even with flocculent application (but this result might have been confounded by very high pH readings, at which flocculation is less likely to occur). Oreochromis mossambicus removed some of this algae, but the pH was too high for tilapia culture. It was not possible to moderate the increase in pH by keeping tanks in the dark and thus preventing photosynthesis; but pH fluctuation in HRAP effluent could be moderated using CO2 sparging in an attempt to make the environment more hospitable for tilapia (the average pH that was moderated with CO2 was 8.43±0.06, whereas the unmoderated average was 10.65±0.06). However, pH moderation using CO2 sparging did not increase the rate at which algae were removed by O. mossambicus; rather, it compromised O2 concentration which dropped to 4.17±1.26 mg/l after five hours of CO2 sparging, whereas it increased to 20.50±1.41 mg/l in treatments with unadjusted pH over the same period. Fish can be used to remove algae from treated effluent, and Oreochromis mossambicus remains a recommended species. Future work needs to investigate moderating fluctuations in pH and O2 concentration to further facilitate this method of algae removal.
- Full Text:
- Date Issued: 2019
- Authors: Nombembe, Lwazi
- Date: 2019
- Subjects: Clarias gariepinus -- Food , Mozambique tilapia -- Food , Water -- Purification -- South Africa , Algae -- Biotechnology -- South Africa , Microalgae -- Biotechnology-- South Africa , Brewery waste -- South Africa , Fish culture -- South Africa
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/95745 , vital:31194
- Description: The removal of microalgae from high rate algal ponds (HRAP) in waste-water treatment systems remains a constraint to their use in effluent treatment systems. Conventional algae harvesting methods often have high energy demands, take up lots of space, are expensive to operate or are time consuming. The aim of the study was to determine if fish such as Clarias gariepinus and Oreochromis mossambicus, could be used to remove microalgae from waste-water treatment ponds (in the absence/presence of a flocculent in the former and in the absence or presence of pH moderation in the latter), and to investigate the subsequent influence of algae concentration on several water quality parameters. The age of Clarias gariepinus (3-12 months) had a positive relationship with the distance between gill rakers (98.27 to 163.34 μm; y=90.576+4.823*x: R²=0.549; F(1,18)=21.867; p<0.001) and these data suggested that these fish might be efficient at removing algae from HRAP effluent. However, this was not the case, even with flocculent application (but this result might have been confounded by very high pH readings, at which flocculation is less likely to occur). Oreochromis mossambicus removed some of this algae, but the pH was too high for tilapia culture. It was not possible to moderate the increase in pH by keeping tanks in the dark and thus preventing photosynthesis; but pH fluctuation in HRAP effluent could be moderated using CO2 sparging in an attempt to make the environment more hospitable for tilapia (the average pH that was moderated with CO2 was 8.43±0.06, whereas the unmoderated average was 10.65±0.06). However, pH moderation using CO2 sparging did not increase the rate at which algae were removed by O. mossambicus; rather, it compromised O2 concentration which dropped to 4.17±1.26 mg/l after five hours of CO2 sparging, whereas it increased to 20.50±1.41 mg/l in treatments with unadjusted pH over the same period. Fish can be used to remove algae from treated effluent, and Oreochromis mossambicus remains a recommended species. Future work needs to investigate moderating fluctuations in pH and O2 concentration to further facilitate this method of algae removal.
- Full Text:
- Date Issued: 2019
The time course changes in selected fatigue indicators in moderately trained participants
- Authors: Maduna, Bongani Cyprian
- Date: 2019
- Subjects: Athletes -- Health and hygiene , Fatigue , Athletes -- Physiology
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/76354 , vital:30553
- Description: Background: During daily physical activities, individuals will encounter some level of fatigue. This is especially true for athletes who exert their bodies to achieve superior performance and attempt to delay the onset of fatigue as far as possible. However, fatigue is a progressive process that is part of physical exercise. It is therefore, important to understand the various factors associated with fatigue. Objective: The purpose of this research project was to observe the changes in perceptual, physiological responses, and workload while participants performed a fatiguing cycling exercise task. In order to observe the aforementioned changes, one of the three variables, either perceptual, physiological responses or workload was kept constant, while the other two were observed as dependent variables. There were three test conditions, which permitted each variable to be kept constant in at least one of the three test conditions. Methods: Thirty-six apparently healthy university students were recruited for the study. The exercise was of 35 minute duration for each condition, with the data collection for HR (physiological response), RPE (perceptual response), and power output (workload) occurring at two minute intervals from the eighth minute until the thirtieth minute. The participants were required to perform a peak-power-output test in order set the relative performance ranges for each participant in order to elicit an observable fatigue response from all the participants. The three conditions included constant HR response, constant workload, and constant RPE response where each participant performed all of the above conditions on separate days.Results: The perceptual (RPE) response increased significantly (p<0.05) over time in all three test conditions, even under the constant RPE condition where it was expected to remain unchanged throughout the test duration. The HR response only demonstrated a significant (p<0.05) increase over time under the constant workload condition. Lastly, workload remained constant in all three testing conditions. Conclusion: Participants may be able to exercise for longer periods during sub-maximal exercise if they disregard the RPE warning response. In the current study, the RPE response illustrated that participants were being exerted more over time; however, the participants still had physiological and workload capacity to continue exercising. This research project has confirmed the fact that fatigue is a multifaceted phenomenon. Furthermore, it has been illustrated that RPE response alone as an indicator of fatigue onset may be misleading as participants did not breach the HR and workload steady state during the current research. Therefore, it may be more appropriate to assess fatigue onset through the assessment of more than one fatigue variable in order to ensure increased accuracy of the participants’ fatigue state assessment.
- Full Text:
- Date Issued: 2019
- Authors: Maduna, Bongani Cyprian
- Date: 2019
- Subjects: Athletes -- Health and hygiene , Fatigue , Athletes -- Physiology
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/76354 , vital:30553
- Description: Background: During daily physical activities, individuals will encounter some level of fatigue. This is especially true for athletes who exert their bodies to achieve superior performance and attempt to delay the onset of fatigue as far as possible. However, fatigue is a progressive process that is part of physical exercise. It is therefore, important to understand the various factors associated with fatigue. Objective: The purpose of this research project was to observe the changes in perceptual, physiological responses, and workload while participants performed a fatiguing cycling exercise task. In order to observe the aforementioned changes, one of the three variables, either perceptual, physiological responses or workload was kept constant, while the other two were observed as dependent variables. There were three test conditions, which permitted each variable to be kept constant in at least one of the three test conditions. Methods: Thirty-six apparently healthy university students were recruited for the study. The exercise was of 35 minute duration for each condition, with the data collection for HR (physiological response), RPE (perceptual response), and power output (workload) occurring at two minute intervals from the eighth minute until the thirtieth minute. The participants were required to perform a peak-power-output test in order set the relative performance ranges for each participant in order to elicit an observable fatigue response from all the participants. The three conditions included constant HR response, constant workload, and constant RPE response where each participant performed all of the above conditions on separate days.Results: The perceptual (RPE) response increased significantly (p<0.05) over time in all three test conditions, even under the constant RPE condition where it was expected to remain unchanged throughout the test duration. The HR response only demonstrated a significant (p<0.05) increase over time under the constant workload condition. Lastly, workload remained constant in all three testing conditions. Conclusion: Participants may be able to exercise for longer periods during sub-maximal exercise if they disregard the RPE warning response. In the current study, the RPE response illustrated that participants were being exerted more over time; however, the participants still had physiological and workload capacity to continue exercising. This research project has confirmed the fact that fatigue is a multifaceted phenomenon. Furthermore, it has been illustrated that RPE response alone as an indicator of fatigue onset may be misleading as participants did not breach the HR and workload steady state during the current research. Therefore, it may be more appropriate to assess fatigue onset through the assessment of more than one fatigue variable in order to ensure increased accuracy of the participants’ fatigue state assessment.
- Full Text:
- Date Issued: 2019
Statistical Analysis of the Radio-Interferometric Measurement Equation, a derived adaptive weighting scheme, and applications to LOFAR-VLBI observation of the Extended Groth Strip
- Authors: Bonnassieux, Etienne
- Date: 2019
- Subjects: Radio astronomy , Astrophysics , Astrophysics -- Instruments -- Calibration , Imaging systems in astronomy , Radio interferometers , Radio telescopes , Astronomy -- Observations
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/93789 , vital:30942
- Description: J.R.R Tolkien wrote, in his Mythopoeia, that “He sees no stars who does not see them first, of living silver made that sudden burst, to flame like flowers beneath the ancient song”. In his defense of myth-making, he formulates the argument that the attribution of meaning is an act of creation - that “trees are not ‘trees’ until so named and seen” - and that this capacity for creation defines the human creature. The scientific endeavour, in this context, can be understood as a social expression of a fundamental feature of humanity, and from this endeavour flows much understanding. This thesis, one thread among many, focuses on the study of astronomical objects as seen by the radio waves they emit. What are radio waves? Electromagnetic waves were theorised by James Clerk Maxwell (Maxwell 1864) in his great theoretical contribution to modern physics, their speed matching the speed of light as measured by Ole Christensen R0mer and, later, James Bradley. It was not until Heinrich Rudolf Hertz’s 1887 experiment that these waves were measured in a laboratory, leading to the dawn of radio communications - and, later, radio astronomy. The link between radio waves and light was one of association: light is known to behave as a wave (Young double-slit experiment), with the same propagation speed as electromagnetic radiation. Light “proper” is also known to exist beyond the optical regime: Herschel’s experiment shows that when diffracted through a prism, sunlight warms even those parts of a desk which are not observed to be lit (first evidence of infrared light). The link between optical light and unseen electromagnetic radiation is then an easy step to make, and one confirmed through countless technological applications (e.g. optical fiber to name but one). And as soon as this link is established, a question immediately comes to the mind of the astronomer: what does the sky, our Universe, look like to the radio “eye”? Radio astronomy has a short but storied history: from Karl Jansky’s serendipitous observation of the centre of the Milky Way, which outshines our Sun in the radio regime, in 1933, to Grote Reber’s hand-built back-yard radio antenna in 1937, which successfully detected radio emission from the Milky Way itself, to such monumental projects as the Square Kilometer Array and its multiple pathfinders, it has led to countless discoveries and the opening of a truly new window on the Universe. The work presented in this thesis is a contribution to this discipline - the culmination of three years of study, which is a rather short time to get a firm grasp of radio interferometry both in theory and in practice. The need for robust, automated methods - which are improving daily, thanks to the tireless labour of the scientists in the field - is becoming ever stronger as the SKA approaches, looming large on the horizon; but even today, in the precursor era of LOFAR, MeerKAT and other pathfinders, it is keenly felt. When I started my doctorate, the sheer scale of the task at hand felt overwhelming - to actually be able to contribute to its resolution seemed daunting indeed! Thankfully, as the saying goes, no society sets for itself material goals which it cannot achieve. This thesis took place at an exciting time for radio interferometry: at the start of my doctorate, the LOFAR international stations were - to my knowledge - only beginning to be used, and even then, only tentatively; MeerKAT had not yet shown its first light; the techniques used throughout my work were still being developed. At the time of writing, great strides have been made. One of the greatest technical challenges of LOFAR - imaging using the international stations - is starting to become reality. This technical challenge is the key problem that this thesis set out to address. While we only achieved partial success so far, it is a testament to the difficulty of the task that it is not yet truly resolved. One of the major results of this thesis is a model of a bright resolved source near a famous extragalactic field: properly modeling this source not only allows the use of international LOFAR stations, but also grants deeper access to the extragalactic field itself, which is otherwise polluted by the 3C source’s sidelobes. This result was only achieved thanks to the other major result of this thesis: the development of a theoretical framework with which to better understand the effect of calibration errors on images made from interferometric data, and an algorithm to strongly mitigate them. The structure of this manuscript is as follows: we begin with an introduction to radio interferometry, LOFAR, and the emission mechanisms which dominate for our field of interest. These introductions are primarily intended to give a brief overview of the technical aspects of the data reduced in this thesis. We follow with an overview of the Measurement Equation formalism, which underpins our theoretical work. This is the keystone of this thesis. We then show the theoretical work that was developed as part of the research work done during the doctorate - which was published in Astronomy & Astrophysics. Its practical application - a quality-based weighting scheme - is used throughout our data reduction. This data reduction is the next topic of this thesis: we contextualise the scientific interest of the data we reduce, and explain both the methods and the results we achieve.
- Full Text:
- Date Issued: 2019
- Authors: Bonnassieux, Etienne
- Date: 2019
- Subjects: Radio astronomy , Astrophysics , Astrophysics -- Instruments -- Calibration , Imaging systems in astronomy , Radio interferometers , Radio telescopes , Astronomy -- Observations
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/93789 , vital:30942
- Description: J.R.R Tolkien wrote, in his Mythopoeia, that “He sees no stars who does not see them first, of living silver made that sudden burst, to flame like flowers beneath the ancient song”. In his defense of myth-making, he formulates the argument that the attribution of meaning is an act of creation - that “trees are not ‘trees’ until so named and seen” - and that this capacity for creation defines the human creature. The scientific endeavour, in this context, can be understood as a social expression of a fundamental feature of humanity, and from this endeavour flows much understanding. This thesis, one thread among many, focuses on the study of astronomical objects as seen by the radio waves they emit. What are radio waves? Electromagnetic waves were theorised by James Clerk Maxwell (Maxwell 1864) in his great theoretical contribution to modern physics, their speed matching the speed of light as measured by Ole Christensen R0mer and, later, James Bradley. It was not until Heinrich Rudolf Hertz’s 1887 experiment that these waves were measured in a laboratory, leading to the dawn of radio communications - and, later, radio astronomy. The link between radio waves and light was one of association: light is known to behave as a wave (Young double-slit experiment), with the same propagation speed as electromagnetic radiation. Light “proper” is also known to exist beyond the optical regime: Herschel’s experiment shows that when diffracted through a prism, sunlight warms even those parts of a desk which are not observed to be lit (first evidence of infrared light). The link between optical light and unseen electromagnetic radiation is then an easy step to make, and one confirmed through countless technological applications (e.g. optical fiber to name but one). And as soon as this link is established, a question immediately comes to the mind of the astronomer: what does the sky, our Universe, look like to the radio “eye”? Radio astronomy has a short but storied history: from Karl Jansky’s serendipitous observation of the centre of the Milky Way, which outshines our Sun in the radio regime, in 1933, to Grote Reber’s hand-built back-yard radio antenna in 1937, which successfully detected radio emission from the Milky Way itself, to such monumental projects as the Square Kilometer Array and its multiple pathfinders, it has led to countless discoveries and the opening of a truly new window on the Universe. The work presented in this thesis is a contribution to this discipline - the culmination of three years of study, which is a rather short time to get a firm grasp of radio interferometry both in theory and in practice. The need for robust, automated methods - which are improving daily, thanks to the tireless labour of the scientists in the field - is becoming ever stronger as the SKA approaches, looming large on the horizon; but even today, in the precursor era of LOFAR, MeerKAT and other pathfinders, it is keenly felt. When I started my doctorate, the sheer scale of the task at hand felt overwhelming - to actually be able to contribute to its resolution seemed daunting indeed! Thankfully, as the saying goes, no society sets for itself material goals which it cannot achieve. This thesis took place at an exciting time for radio interferometry: at the start of my doctorate, the LOFAR international stations were - to my knowledge - only beginning to be used, and even then, only tentatively; MeerKAT had not yet shown its first light; the techniques used throughout my work were still being developed. At the time of writing, great strides have been made. One of the greatest technical challenges of LOFAR - imaging using the international stations - is starting to become reality. This technical challenge is the key problem that this thesis set out to address. While we only achieved partial success so far, it is a testament to the difficulty of the task that it is not yet truly resolved. One of the major results of this thesis is a model of a bright resolved source near a famous extragalactic field: properly modeling this source not only allows the use of international LOFAR stations, but also grants deeper access to the extragalactic field itself, which is otherwise polluted by the 3C source’s sidelobes. This result was only achieved thanks to the other major result of this thesis: the development of a theoretical framework with which to better understand the effect of calibration errors on images made from interferometric data, and an algorithm to strongly mitigate them. The structure of this manuscript is as follows: we begin with an introduction to radio interferometry, LOFAR, and the emission mechanisms which dominate for our field of interest. These introductions are primarily intended to give a brief overview of the technical aspects of the data reduced in this thesis. We follow with an overview of the Measurement Equation formalism, which underpins our theoretical work. This is the keystone of this thesis. We then show the theoretical work that was developed as part of the research work done during the doctorate - which was published in Astronomy & Astrophysics. Its practical application - a quality-based weighting scheme - is used throughout our data reduction. This data reduction is the next topic of this thesis: we contextualise the scientific interest of the data we reduce, and explain both the methods and the results we achieve.
- Full Text:
- Date Issued: 2019
Who is a refugee? a philosophical account
- Authors: Oteng, Onalethata
- Date: 2019
- Subjects: Refugees -- Philosophy , Refugees -- Government policy , Refugees -- Legal status, laws, etc. , United Nations Conference of Plenipotentiaries on the Status of Refugees and Stateless Persons (1951 : Geneva, Switzerland)
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/92854 , vital:30755
- Description: This thesis comprises two sections: the first section considers who is a refugee and who is not a refugee; and the second section illustrates the necessity of amending the present international laws and conventions to include other people who should also be recognised as refugees. The critical issue regarding the definition of a refugee, is whether or not there is adequate evidence concerning the current understanding of the notion of who a refugee is. Therefore, in order to reach these goals, this thesis is divided into five chapters. The first chapter explores the definitions pertaining to what it means to be a refugee. The second chapter takes into account the different types of victims that are excluded from the notion of refugee. The third chapter considers the consequences of having a restricted understanding who qualifies to be a refugee. The fourth chapter provides alternative solutions that would assist in providing a more comprehensive definition. Furthermore, chapter five discusses the necessity to further expand the refugee concept to include other forms of victims that are not already included. Overall, this thesis seeks to support expansion of the term ‘refugee‘ since the reasons for forced migration have changed, and individuals are not only fleeing from individual persecution, for instance, but from other situations that can be considered to fall under forced migration. Consequently, extending the definition of the term ‘refugee‘ should occur, because refusing to consider revising the term amounts to a refusal to show acceptance and empathy to today‘s other migrants who are also experiencing survival challenges in their home countries.
- Full Text:
- Date Issued: 2019
- Authors: Oteng, Onalethata
- Date: 2019
- Subjects: Refugees -- Philosophy , Refugees -- Government policy , Refugees -- Legal status, laws, etc. , United Nations Conference of Plenipotentiaries on the Status of Refugees and Stateless Persons (1951 : Geneva, Switzerland)
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/92854 , vital:30755
- Description: This thesis comprises two sections: the first section considers who is a refugee and who is not a refugee; and the second section illustrates the necessity of amending the present international laws and conventions to include other people who should also be recognised as refugees. The critical issue regarding the definition of a refugee, is whether or not there is adequate evidence concerning the current understanding of the notion of who a refugee is. Therefore, in order to reach these goals, this thesis is divided into five chapters. The first chapter explores the definitions pertaining to what it means to be a refugee. The second chapter takes into account the different types of victims that are excluded from the notion of refugee. The third chapter considers the consequences of having a restricted understanding who qualifies to be a refugee. The fourth chapter provides alternative solutions that would assist in providing a more comprehensive definition. Furthermore, chapter five discusses the necessity to further expand the refugee concept to include other forms of victims that are not already included. Overall, this thesis seeks to support expansion of the term ‘refugee‘ since the reasons for forced migration have changed, and individuals are not only fleeing from individual persecution, for instance, but from other situations that can be considered to fall under forced migration. Consequently, extending the definition of the term ‘refugee‘ should occur, because refusing to consider revising the term amounts to a refusal to show acceptance and empathy to today‘s other migrants who are also experiencing survival challenges in their home countries.
- Full Text:
- Date Issued: 2019
Mesoscale alongshore and cross-shore transport and settlement of invertebrate larvae on the south east coast of South Africa
- Authors: Dyantyi, Siphelele Buntu
- Date: 2019
- Subjects: Marine invertebrates -- Larvae -- Dispersal -- South Africa -- Eastern Cape , Benthic animals -- Larvae -- Dispersal -- South Africa -- Eastern Cape , Mexilhao mussel -- Larvae -- Dispersal -- South Africa -- Eastern Cape , Mytilus galloprovincialis -- Larvae -- Dispersal -- South Africa -- Eastern Cape , Oysters -- Larvae -- Dispersal -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/97007 , vital:31386
- Description: Pelagic larval stages of most marine benthic species are important in maintaining coastal populations of adults. Several physio-chemical factors such as currents, winds, larval behaviour and time have an influence on the dispersal and transport of larvae to the adult habitat but their role is however still poorly understood. The aim of this study was to investigate the alongshore and cross-shore transport and temporal delivery of invertebrate larvae at four sites (Kenton on Sea, Cannon Rocks, Schoenmakerskop and Cape St Francis) along the south east coast of Eastern Cape, South Africa. Larval distribution of several taxa was determined during two sampling periods, by collecting water samples at nearshore line transects (3 distances: 900m, 1500m and 2400m – perpendicular to the shore) and at three depths (surface, thermocline/middle, bottom), which ranged from 15m (inshore) to 50m (offshore). Physical properties (current speed and direction, dissolved oxygen, fluorescence, turbidity, temperature, salinity, pH, pressure, density and conductivity) were measured and coupled in order to further understand larval distribution. The larvae were analysed as both total abundance and separately as the abundances of a variety of taxa which were: Perna perna, Mytilus galloprovincialis, oysters, early and late nauplii and cyprids. Larval settlement and recruitment on the rocky shores were measured by monthly deployment and collection of 20 (10 each for barnacles and mussels) artificial collectors at each site, which were preserved in ethanol or frozen for further processing. Multiple Permutational Multivariate Analysis of Variance (PERMANOVA) analyses were used to test the effects of site, depth and distance from the shore for the nearshore larvae (taxa analysed separately). In addition, a distance based linear model (distLM) was performed to analyse the relationship between the total larval abundance and the above mentioned physical variables. Multiple two-way analyses of variance (ANOVA) were performed to test the effects of months and sites on the settlement and recruitment of the larvae (P. perna, M. galloprovincialis, other bivalves, cyprids and juvenile barnacles) arriving on the shore. For the nearshore larval distribution, results from the PERMANOVAs revealed that most taxa showed a significant site and depth interactions with the exception of ‘early nauplii’ taxon. Also nearly all taxa were found within the thermocline, besides ‘oyster’ and ‘cyprids’ which were located at thermocline or bottom. Larvae were also located at variable distances from the shore, with most occurring at the offshore stations. Furthermore, there was a geographical separation of larval abundance according to sites, with most larvae located at Cannon Rocks and Kenton on Sea and least at Schoenmakerskop and Cape St Francis. For the settlement and recruitment, most taxa showed a seasonal trend, with the highest abundance of settlers and recruits expectedly appearing during the summer months of the sampling period. Additionally there was a site effect for most taxa (P. perna, M. galloprovincialis, other bivalves and juvenile barnacles), where settlers and recruits were mostly found at Cannon Rocks. Significant differences in abundance of settlers and recruits amongst the four sites indicate spatial and temporal variability for the targeted 180km stretch of coast. Overall for this study, taxon and ontogenetic stage of larvae were important in the distribution and abundance of larvae. Throughout the time frame of nearshore and intertidal sampling, Cannon Rocks consistently resulted as a ‘hot spot’ for larval abundance, settlement and recruitment, while a broad west to east separation was also observed. These results hence highlight that within this stretch of c.180km coast, time, taxon, ontogeny and post-settlement factors influence early dynamics of benthic populations.
- Full Text:
- Date Issued: 2019
- Authors: Dyantyi, Siphelele Buntu
- Date: 2019
- Subjects: Marine invertebrates -- Larvae -- Dispersal -- South Africa -- Eastern Cape , Benthic animals -- Larvae -- Dispersal -- South Africa -- Eastern Cape , Mexilhao mussel -- Larvae -- Dispersal -- South Africa -- Eastern Cape , Mytilus galloprovincialis -- Larvae -- Dispersal -- South Africa -- Eastern Cape , Oysters -- Larvae -- Dispersal -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/97007 , vital:31386
- Description: Pelagic larval stages of most marine benthic species are important in maintaining coastal populations of adults. Several physio-chemical factors such as currents, winds, larval behaviour and time have an influence on the dispersal and transport of larvae to the adult habitat but their role is however still poorly understood. The aim of this study was to investigate the alongshore and cross-shore transport and temporal delivery of invertebrate larvae at four sites (Kenton on Sea, Cannon Rocks, Schoenmakerskop and Cape St Francis) along the south east coast of Eastern Cape, South Africa. Larval distribution of several taxa was determined during two sampling periods, by collecting water samples at nearshore line transects (3 distances: 900m, 1500m and 2400m – perpendicular to the shore) and at three depths (surface, thermocline/middle, bottom), which ranged from 15m (inshore) to 50m (offshore). Physical properties (current speed and direction, dissolved oxygen, fluorescence, turbidity, temperature, salinity, pH, pressure, density and conductivity) were measured and coupled in order to further understand larval distribution. The larvae were analysed as both total abundance and separately as the abundances of a variety of taxa which were: Perna perna, Mytilus galloprovincialis, oysters, early and late nauplii and cyprids. Larval settlement and recruitment on the rocky shores were measured by monthly deployment and collection of 20 (10 each for barnacles and mussels) artificial collectors at each site, which were preserved in ethanol or frozen for further processing. Multiple Permutational Multivariate Analysis of Variance (PERMANOVA) analyses were used to test the effects of site, depth and distance from the shore for the nearshore larvae (taxa analysed separately). In addition, a distance based linear model (distLM) was performed to analyse the relationship between the total larval abundance and the above mentioned physical variables. Multiple two-way analyses of variance (ANOVA) were performed to test the effects of months and sites on the settlement and recruitment of the larvae (P. perna, M. galloprovincialis, other bivalves, cyprids and juvenile barnacles) arriving on the shore. For the nearshore larval distribution, results from the PERMANOVAs revealed that most taxa showed a significant site and depth interactions with the exception of ‘early nauplii’ taxon. Also nearly all taxa were found within the thermocline, besides ‘oyster’ and ‘cyprids’ which were located at thermocline or bottom. Larvae were also located at variable distances from the shore, with most occurring at the offshore stations. Furthermore, there was a geographical separation of larval abundance according to sites, with most larvae located at Cannon Rocks and Kenton on Sea and least at Schoenmakerskop and Cape St Francis. For the settlement and recruitment, most taxa showed a seasonal trend, with the highest abundance of settlers and recruits expectedly appearing during the summer months of the sampling period. Additionally there was a site effect for most taxa (P. perna, M. galloprovincialis, other bivalves and juvenile barnacles), where settlers and recruits were mostly found at Cannon Rocks. Significant differences in abundance of settlers and recruits amongst the four sites indicate spatial and temporal variability for the targeted 180km stretch of coast. Overall for this study, taxon and ontogenetic stage of larvae were important in the distribution and abundance of larvae. Throughout the time frame of nearshore and intertidal sampling, Cannon Rocks consistently resulted as a ‘hot spot’ for larval abundance, settlement and recruitment, while a broad west to east separation was also observed. These results hence highlight that within this stretch of c.180km coast, time, taxon, ontogeny and post-settlement factors influence early dynamics of benthic populations.
- Full Text:
- Date Issued: 2019
A multi-threading software countermeasure to mitigate side channel analysis in the time domain
- Authors: Frieslaar, Ibraheem
- Date: 2019
- Subjects: Computer security , Data encryption (Computer science) , Noise generators (Electronics)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/71152 , vital:29790
- Description: This research is the first of its kind to investigate the utilisation of a multi-threading software-based countermeasure to mitigate Side Channel Analysis (SCA) attacks, with a particular focus on the AES-128 cryptographic algorithm. This investigation is novel, as there has not been a software-based countermeasure relying on multi-threading to our knowledge. The research has been tested on the Atmel microcontrollers, as well as a more fully featured system in the form of the popular Raspberry Pi that utilises the ARM7 processor. The main contributions of this research is the introduction of a multi-threading software based countermeasure used to mitigate SCA attacks on both an embedded device and a Raspberry Pi. These threads are comprised of various mathematical operations which are utilised to generate electromagnetic (EM) noise resulting in the obfuscation of the execution of the AES-128 algorithm. A novel EM noise generator known as the FRIES noise generator is implemented to obfuscate data captured in the EM field. FRIES comprises of hiding the execution of AES-128 algorithm within the EM noise generated by the 512 Secure Hash Algorithm (SHA) from the libcrypto++ and OpenSSL libraries. In order to evaluate the proposed countermeasure, a novel attack methodology was developed where the entire secret AES-128 encryption key was recovered from a Raspberry Pi, which has not been achieved before. The FRIES noise generator was pitted against this new attack vector and other known noise generators. The results exhibited that the FRIES noise generator withstood this attack whilst other existing techniques still leaked out secret information. The visual location of the AES-128 encryption algorithm in the EM spectrum and key recovery was prevented. These results demonstrated that the proposed multi-threading software based countermeasure was able to be resistant to existing and new forms of attacks, thus verifying that a multi-threading software based countermeasure can serve to mitigate SCA attacks.
- Full Text:
- Date Issued: 2019
- Authors: Frieslaar, Ibraheem
- Date: 2019
- Subjects: Computer security , Data encryption (Computer science) , Noise generators (Electronics)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/71152 , vital:29790
- Description: This research is the first of its kind to investigate the utilisation of a multi-threading software-based countermeasure to mitigate Side Channel Analysis (SCA) attacks, with a particular focus on the AES-128 cryptographic algorithm. This investigation is novel, as there has not been a software-based countermeasure relying on multi-threading to our knowledge. The research has been tested on the Atmel microcontrollers, as well as a more fully featured system in the form of the popular Raspberry Pi that utilises the ARM7 processor. The main contributions of this research is the introduction of a multi-threading software based countermeasure used to mitigate SCA attacks on both an embedded device and a Raspberry Pi. These threads are comprised of various mathematical operations which are utilised to generate electromagnetic (EM) noise resulting in the obfuscation of the execution of the AES-128 algorithm. A novel EM noise generator known as the FRIES noise generator is implemented to obfuscate data captured in the EM field. FRIES comprises of hiding the execution of AES-128 algorithm within the EM noise generated by the 512 Secure Hash Algorithm (SHA) from the libcrypto++ and OpenSSL libraries. In order to evaluate the proposed countermeasure, a novel attack methodology was developed where the entire secret AES-128 encryption key was recovered from a Raspberry Pi, which has not been achieved before. The FRIES noise generator was pitted against this new attack vector and other known noise generators. The results exhibited that the FRIES noise generator withstood this attack whilst other existing techniques still leaked out secret information. The visual location of the AES-128 encryption algorithm in the EM spectrum and key recovery was prevented. These results demonstrated that the proposed multi-threading software based countermeasure was able to be resistant to existing and new forms of attacks, thus verifying that a multi-threading software based countermeasure can serve to mitigate SCA attacks.
- Full Text:
- Date Issued: 2019
The preparation of BODIPY and porphyrin dyes and their cyclodextrin inclusion complexes and Pluronic® F-127 encapsulation micelles for use in PDT and PACT
- Authors: Molupe, Nthabeleng
- Date: 2019
- Subjects: Dyes and dyeing -- Chemistry , Drug delivery systems , Fluorescence spectroscopy , Cancer -- Photochemotherapy , Photosensitizing compounds -- Therapeutic use , Cyclodextrins -- Biotechnology , Nanoparticles
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/117574 , vital:34528
- Description: Several novel BODIPY dyes ((4,4′-difluoro-1,7-tetramethyl-3,5-(3-dithiophene)-2,6-diiodo-8-(4-dimethylamino)-4-bora-3a,4a-diaza-s-indacene (1c), 4,4′-difluoro-1,7-tetramethyl-3,5-(3 dithiophene)-2,6-diiodo-8-(4-methylthio)-4-bora-3a,4a-diaza-s-indacene (3c) and 4,4′-difluoro-1,7-tetramethyl-3,5-(4-dibenzyloxybenzene)-2,6-diiodo-8-(4-methylbenzoate)-4 bora-3a,4a-diaza-s-indacene (4c)) and porphyrins (tetraacenaphthylporphyrin (7a) and Sn(IV) tetraacenaphthylporphyrin (7b)) were synthesized and characterized. Previously reported BODIPY dyes (4,4′-difluoro-1,7-tetramethyl-3,5-(2-dihydroxy)-2,6-diiodo-8-(4-bromo)-4-bora-3a,4a-diaza-s-indacene (5) and 4,4′-difluoro-1,7-tetramethyl-3,5-(2-dithiophene)-2,6-diiodo-8-(phenyl)-4-bora-3a,4a-diaza-s-indacene (6)) were also used. Pluronic® F-127 and cyclodextrins were used as solubilizing drug delivery agents for the synthesized BODIPY dyes. The encapsulation of BODIPY dyes with Pluronic® F-127 micelles improved the water solubility of the BODIPY 5. Further modification of Pluronic® F-127 by coating with folate-functionalized chitosan for targeted delivery of BODIPY 1c and 6 was explored. The BODIPY dyes and their encapsulation complexes exhibited significant inhibition of human MCF-7 breast cancer cell growth. When cyclodextrins were used as nanocarriers, the inclusion complexes of BODIPY 4c with mβCD were found to enhance the water-solubility of the dye. Greater photoinactivation of Staphylococcus aureus was observed for the inclusion complexes when compared to the effect of solutions of non-complexed BODIPY 4c. The cyclodextrin inclusion complexes of porphyrin 7b with mβCD were also found to enhance the water-solubility of 7b. When the photodynamic effect was evaluated, solutions of the porphyrin alone and their inclusion complexes were found to have significant photodynamic effects against human MCF-7 breast cancer cells.
- Full Text:
- Date Issued: 2019
- Authors: Molupe, Nthabeleng
- Date: 2019
- Subjects: Dyes and dyeing -- Chemistry , Drug delivery systems , Fluorescence spectroscopy , Cancer -- Photochemotherapy , Photosensitizing compounds -- Therapeutic use , Cyclodextrins -- Biotechnology , Nanoparticles
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/117574 , vital:34528
- Description: Several novel BODIPY dyes ((4,4′-difluoro-1,7-tetramethyl-3,5-(3-dithiophene)-2,6-diiodo-8-(4-dimethylamino)-4-bora-3a,4a-diaza-s-indacene (1c), 4,4′-difluoro-1,7-tetramethyl-3,5-(3 dithiophene)-2,6-diiodo-8-(4-methylthio)-4-bora-3a,4a-diaza-s-indacene (3c) and 4,4′-difluoro-1,7-tetramethyl-3,5-(4-dibenzyloxybenzene)-2,6-diiodo-8-(4-methylbenzoate)-4 bora-3a,4a-diaza-s-indacene (4c)) and porphyrins (tetraacenaphthylporphyrin (7a) and Sn(IV) tetraacenaphthylporphyrin (7b)) were synthesized and characterized. Previously reported BODIPY dyes (4,4′-difluoro-1,7-tetramethyl-3,5-(2-dihydroxy)-2,6-diiodo-8-(4-bromo)-4-bora-3a,4a-diaza-s-indacene (5) and 4,4′-difluoro-1,7-tetramethyl-3,5-(2-dithiophene)-2,6-diiodo-8-(phenyl)-4-bora-3a,4a-diaza-s-indacene (6)) were also used. Pluronic® F-127 and cyclodextrins were used as solubilizing drug delivery agents for the synthesized BODIPY dyes. The encapsulation of BODIPY dyes with Pluronic® F-127 micelles improved the water solubility of the BODIPY 5. Further modification of Pluronic® F-127 by coating with folate-functionalized chitosan for targeted delivery of BODIPY 1c and 6 was explored. The BODIPY dyes and their encapsulation complexes exhibited significant inhibition of human MCF-7 breast cancer cell growth. When cyclodextrins were used as nanocarriers, the inclusion complexes of BODIPY 4c with mβCD were found to enhance the water-solubility of the dye. Greater photoinactivation of Staphylococcus aureus was observed for the inclusion complexes when compared to the effect of solutions of non-complexed BODIPY 4c. The cyclodextrin inclusion complexes of porphyrin 7b with mβCD were also found to enhance the water-solubility of 7b. When the photodynamic effect was evaluated, solutions of the porphyrin alone and their inclusion complexes were found to have significant photodynamic effects against human MCF-7 breast cancer cells.
- Full Text:
- Date Issued: 2019
Ndonakele
- Authors: Ngcelwane, Ayabulela
- Date: 2019
- Subjects: Xhosa fiction , Xhosa poetry
- Language: English , Xhosa
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/92721 , vital:30742
- Description: Le thisisi yingqokelela yemibongo egxile kwimingeni ejamelene noluntu kwimimandla efana neelokishi. Unobangela wale mingeni yintswelo-ngqesho, ukungafumani zindlu, ubundlobongela, isimo sezepolitiki, njalo-njalo. Imisebenzi kaRonnie Kasrils ngakumbi kwincwadi yakhe ethi A simple man, uRustum Siyongwana – Ubulumko bezinja uS. S. Mema – Umnxeba Wobomi, nezinye, zibe nefuthe kakhulu kule thisisi. Indlela abasebenzisa ngayo ulwimi lwemihla ngemihla, maxawambi bade basebenzise nesakhono esisetyenziswa ezintsomini ezifana nokusebenzisa abalinganiswa abazizilwanyana bekwekwa umba onobuzaza. Injongo yalo msebenzi kukugqamisa ukubaluleka kwentlalontle yoluntu, nokuvuselela isazela koongxowa ayibotshwa. Yiyo loo nto kuzanywe kangangoko ukusetyenziswa ulwimi olunokufikeleleka nakubani na. , English and Xhoxa versions provided for dual language submission
- Full Text:
- Date Issued: 2019
- Authors: Ngcelwane, Ayabulela
- Date: 2019
- Subjects: Xhosa fiction , Xhosa poetry
- Language: English , Xhosa
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/92721 , vital:30742
- Description: Le thisisi yingqokelela yemibongo egxile kwimingeni ejamelene noluntu kwimimandla efana neelokishi. Unobangela wale mingeni yintswelo-ngqesho, ukungafumani zindlu, ubundlobongela, isimo sezepolitiki, njalo-njalo. Imisebenzi kaRonnie Kasrils ngakumbi kwincwadi yakhe ethi A simple man, uRustum Siyongwana – Ubulumko bezinja uS. S. Mema – Umnxeba Wobomi, nezinye, zibe nefuthe kakhulu kule thisisi. Indlela abasebenzisa ngayo ulwimi lwemihla ngemihla, maxawambi bade basebenzise nesakhono esisetyenziswa ezintsomini ezifana nokusebenzisa abalinganiswa abazizilwanyana bekwekwa umba onobuzaza. Injongo yalo msebenzi kukugqamisa ukubaluleka kwentlalontle yoluntu, nokuvuselela isazela koongxowa ayibotshwa. Yiyo loo nto kuzanywe kangangoko ukusetyenziswa ulwimi olunokufikeleleka nakubani na. , English and Xhoxa versions provided for dual language submission
- Full Text:
- Date Issued: 2019
Assessing the abundance of non-timber forest products in relation to forest succession on the Wild Coast, South Africa
- Authors: Njwaxu, Afika
- Date: 2019
- Subjects: Non-timber forest products -- South Africa -- Eastern Cape , Forest ecology -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape , Non-timber forest products -- South Africa -- Willowvale , Forest ecology -- South Africa -- Willowvale , Rural development -- South Africa -- Willowvale , Forest plants -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/94090 , vital:30999
- Description: The number of people in the Eastern Cape, South Africa, that are engaging in arable cropping has declined markedly over the last few decades. This is due to a number of factors such as a decrease in human capital because of migration of able-bodied people to urban areas, disinclination to participate in farming, raiding of crops by livestock and lack of equipment. This has resulted in abandoned croplands being invaded by trees and shrubs as the start of forest succession leading to a change in species composition and the ecosystem benefits reaped from these sites. Key amongst these benefits is a variety of non-timber forests products (NTFPs) which are an integral part of livelihoods in the area. The study was conducted in Willowvale, on the Wild Coast, South Africa. Aerial photographs were used to determine when cessation of cropping occurred in local fields and when revegetation began in order to determine the age of old fields. Botanical inventory and Braun-Blanquet scale were used to assess species richness, composition and abundance of vegetation in fields abandoned at different times. Focus groups were used to identify NTFPs found in these old fields, their uses as well as rank their importance to the local people. Results showed an increase in woody cover with time since field abandonment. Species richness also increased with age of the old field with approximately three species gained per decade. When species richness was disaggregated by growth forms, herbaceous plants were abundant in the early stages of succession, shrubs in the mature stages and trees increased steadily with time. A total of 177 species were recorded from 50 plots that were sampled during the ecological data collection. Of these, 70 species (39.6%) were identified by the focus group participants as NTFPs. The participants grouped the NTFPs into six categories namely: food, building, medicinal, craft, cultural and energy. There was an increase of richness of NTFPs with forest succession; however the proportion of NTFPs decreased with age suggesting an increase in non-useful species. This suggests that harvesting from plots of different ages would be the optimal way for local people to get access to a large variety of NTFPs.
- Full Text:
- Date Issued: 2019
- Authors: Njwaxu, Afika
- Date: 2019
- Subjects: Non-timber forest products -- South Africa -- Eastern Cape , Forest ecology -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape , Non-timber forest products -- South Africa -- Willowvale , Forest ecology -- South Africa -- Willowvale , Rural development -- South Africa -- Willowvale , Forest plants -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/94090 , vital:30999
- Description: The number of people in the Eastern Cape, South Africa, that are engaging in arable cropping has declined markedly over the last few decades. This is due to a number of factors such as a decrease in human capital because of migration of able-bodied people to urban areas, disinclination to participate in farming, raiding of crops by livestock and lack of equipment. This has resulted in abandoned croplands being invaded by trees and shrubs as the start of forest succession leading to a change in species composition and the ecosystem benefits reaped from these sites. Key amongst these benefits is a variety of non-timber forests products (NTFPs) which are an integral part of livelihoods in the area. The study was conducted in Willowvale, on the Wild Coast, South Africa. Aerial photographs were used to determine when cessation of cropping occurred in local fields and when revegetation began in order to determine the age of old fields. Botanical inventory and Braun-Blanquet scale were used to assess species richness, composition and abundance of vegetation in fields abandoned at different times. Focus groups were used to identify NTFPs found in these old fields, their uses as well as rank their importance to the local people. Results showed an increase in woody cover with time since field abandonment. Species richness also increased with age of the old field with approximately three species gained per decade. When species richness was disaggregated by growth forms, herbaceous plants were abundant in the early stages of succession, shrubs in the mature stages and trees increased steadily with time. A total of 177 species were recorded from 50 plots that were sampled during the ecological data collection. Of these, 70 species (39.6%) were identified by the focus group participants as NTFPs. The participants grouped the NTFPs into six categories namely: food, building, medicinal, craft, cultural and energy. There was an increase of richness of NTFPs with forest succession; however the proportion of NTFPs decreased with age suggesting an increase in non-useful species. This suggests that harvesting from plots of different ages would be the optimal way for local people to get access to a large variety of NTFPs.
- Full Text:
- Date Issued: 2019
Responding to iconic images of risk through reflexive and narrative enquiry represented in a stratified text for environmental education readers
- Authors: Murphy, Mary
- Date: 2019
- Subjects: Sustainability -- Study and teaching , Environmental education -- Philosophy , Environmental degradation -- Study and teaching , Environmental degradation -- Philosophy , Reflection (Philosophy) , Archer, Margaret S (Margaret Scotford). Structure, agency, and the internal conversation
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/96768 , vital:31318
- Description: This thesis presents a stratified textual strategy to represent meaning developed through reflexive and narrative enquiry of environmental risk. Meaning that emerged in responses to iconic images of risk. Umberto Eco cautioned that iconic images over time become conventional taking over from that which they represent. Representations of risk become embedded through cultural coding. Semiotic theory provided access to the contextual and cultural content of environmental education as experienced during professional work as a radio presenter of “Environmental Matters”, as an environmental educator and activist. Methodological rigour was applied through the application of Margaret Archer's theory of the internal conversation and use of an online content management system. Both the reflexive tool of the internal conversation and the textual mechanism of the blog encouraged commitment to Paul Hart's criteria of trustworthiness and authenticity in the process of building the semiotic structure of the PhD. The Internal Conversation was used as a mediating tool in the PhD process and is presented in practice. Rethinking environmental risk from other species' perspectives through imagined experience was achieved through narrative enquiry. A noted anthropocentric limitation of the inability to interview animals for their experience of human-imposed risk was mitigated through representing the imagined, possible perspectives through story, which invites the reader to join the meaning-making process and open up discussions for and about environmental issues and action. This noted anthropocentrism was evident in debates among the characters about violence and non-violence as a conditioned theme and topic discussed in previous academic research about terrorism in divided societies. The story illustrates how the main character, a penguin called Polo, navigates through emerging meaning within a structure that confronts him with choices that end with a decision to become an agent for change. This story is a narrative example of the morphogenetic process. The multi-textual strategy presents possible methods for the exploration of risk (Vol. 1), reflexivity (Vol. 2) and representation (Vol. 3) for the application and contribution in/to environmental education.
- Full Text:
- Date Issued: 2019
- Authors: Murphy, Mary
- Date: 2019
- Subjects: Sustainability -- Study and teaching , Environmental education -- Philosophy , Environmental degradation -- Study and teaching , Environmental degradation -- Philosophy , Reflection (Philosophy) , Archer, Margaret S (Margaret Scotford). Structure, agency, and the internal conversation
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/96768 , vital:31318
- Description: This thesis presents a stratified textual strategy to represent meaning developed through reflexive and narrative enquiry of environmental risk. Meaning that emerged in responses to iconic images of risk. Umberto Eco cautioned that iconic images over time become conventional taking over from that which they represent. Representations of risk become embedded through cultural coding. Semiotic theory provided access to the contextual and cultural content of environmental education as experienced during professional work as a radio presenter of “Environmental Matters”, as an environmental educator and activist. Methodological rigour was applied through the application of Margaret Archer's theory of the internal conversation and use of an online content management system. Both the reflexive tool of the internal conversation and the textual mechanism of the blog encouraged commitment to Paul Hart's criteria of trustworthiness and authenticity in the process of building the semiotic structure of the PhD. The Internal Conversation was used as a mediating tool in the PhD process and is presented in practice. Rethinking environmental risk from other species' perspectives through imagined experience was achieved through narrative enquiry. A noted anthropocentric limitation of the inability to interview animals for their experience of human-imposed risk was mitigated through representing the imagined, possible perspectives through story, which invites the reader to join the meaning-making process and open up discussions for and about environmental issues and action. This noted anthropocentrism was evident in debates among the characters about violence and non-violence as a conditioned theme and topic discussed in previous academic research about terrorism in divided societies. The story illustrates how the main character, a penguin called Polo, navigates through emerging meaning within a structure that confronts him with choices that end with a decision to become an agent for change. This story is a narrative example of the morphogenetic process. The multi-textual strategy presents possible methods for the exploration of risk (Vol. 1), reflexivity (Vol. 2) and representation (Vol. 3) for the application and contribution in/to environmental education.
- Full Text:
- Date Issued: 2019
The development of high-throughput assays to screen for potential anticancer and antimalarial compounds that target ADP-ribosylation factor 6 and its signalling machineries
- Authors: Khan, Farrah Dilshaad
- Date: 2019
- Subjects: ADP-ribosylation , Proteins -- Metabolism , Nucleoproteins , Malaria -- Chemotherapy , Cancer -- Chemotherapy
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/92952 , vital:30810
- Description: ADP-ribosylation factors (Arfs) are small GTP-binding proteins that cycle between active GTP-bound forms and inactive GDP-bound forms. GDP/GTP cycling is regulated by large families of guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). ArfGEFs activate Arfs by mediating the exchange of GDP for GTP, while ArfGAPs terminate Arf function by stimulating the hydrolysis of the terminal phosphate group of GTP. Arf6 is a major regulator of endocytic trafficking and reorganization of the actin cytoskeleton in eukaryotic organisms. Owing to its participation in wide range of fundamentally distinct cellular processes, Arf6 may be a drug target for cancer and malaria amongst other diseases. As with cancer cells, rapid growth and viability of eukaryotic pathogens likely places a heavy burden on their endocytic pathways and a critical reliance on Arf6 activity. A putative malarial homolog of Arf6 (PfArf6) localises to numerous puncta along the periphery of the parasite in the mature trophozoite life stage of the parasite (T. Swart, MSc dissertation). Owing to highly inefficient parasite transfection procedures and a relative shortage of well described and validated parasite organelle markers, the possible functions of PfArf6 were explored using HeLa cells as a surrogate model for parasites by fluorescence microscopy of cells transfected with GFP-tagged PfArf6. Partial co-localisation was observed with the mammalian markers HsArf6 and LC3, which suggested possible roles in Arf6-dependent endocytosis and autophagy, respectively. While these possible roles are currently under investigation in parasites, an overall long-term goal which was initiated in this study was to determine whether PfArf6 is a valid drug target. To chemically validate PfArf6 as a drug target, a potent inhibitor needs to be identified. This requires the development of assays that may be employed for high-throughput screening of compound libraries. To support this goal, a novel plate-based assay was developed using human Arf6. The assay relies on the selective binding of an Arf effector protein domain (GGA3) fused to glutathione-S-transferase (GST), to His-tagged Arf6 immobilised on a nickel-coated plate. The assay format was developed and could robustly distinguish HsArf6-GDP (inactive) from HsArf6-GTP (active). Furthermore, it could be employed to detect the deactivation of Arf6 by ArfGAP1-stimualted GTP hydrolysis, but not Arf6 activation by ARNO-stimulated GDP/GTP exchange (ARNO is an ArfGEF). The ArfGAP1 deactivation assay was chemically validated using a known ArfGAP inhibitor, QS11. An improved assay was developed that employs JIP4 as an Arf6-specific binding partner instead of GGA3. In addition to superior performance, the alternative assay format could potentially be exploited for cancer drug discovery, since Arf6-JIP4 interaction has been implicated in cancer cell invasion and metastasis. Both assays may be employed to explore alternative ArfGEFs and ArfGAPs that act on Arf6 and contribute to the advancement of cancer. In parallel experiments, where development of PfArf6 assays was the focus, several issues arose. Firstly, we could not prepare GDP- and GTP-bound forms of PfArf6 since EDTA-mediated nucleotide exchange appeared to irreversibly destabilise the protein. However, PfArf6 activation (i.e. the preparation of PfArf6-GTP) was possible when mediated by ARNO and assessed by tryptophan fluorescence kinetic assays, suggesting that PfArf6 may be expressed in GDP-bound form in E. coli. As with human Arf6, ARNO-mediated GDP/GTP exchange on PfArf6 was not detectable in the immobilised PfArf6-GGA interaction GST assay format. However, a more sensitive assay was developed which relies on the use of nickel-horseradish peroxidase to detect the binding of His-tagged PfArf6 to JIP4-GST immobilised on glutathione plates and could detect ARNO-mediated PfArf6 activation. Since we could not prepare PfArf6-GTP (that did not rely on the presence of the ArfGEF, ARNO), malarial ArfGAP deactivation studies were conducted using PfArf1 instead of PfArf6 in the GGA-GST interaction assay. Both PfArfGAP1and PfArfGAP2 stimulated GTP hydrolysis by PfArf1, but only the former was inhibited by the standard human ArfGAP inhibitor, QS11. The development of these simple, cost-effective assays can be used in the high-throughput screening of novel anticancer and antimalarial compounds that target Arf signalling machineries. In theory, the assay could be extended as a tool to identify novel inhibitors of the multitude of Arfs, ArfGEFs and ArfGAPs originating from any organism and hence has broad clinical significance.
- Full Text:
- Date Issued: 2019
- Authors: Khan, Farrah Dilshaad
- Date: 2019
- Subjects: ADP-ribosylation , Proteins -- Metabolism , Nucleoproteins , Malaria -- Chemotherapy , Cancer -- Chemotherapy
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/92952 , vital:30810
- Description: ADP-ribosylation factors (Arfs) are small GTP-binding proteins that cycle between active GTP-bound forms and inactive GDP-bound forms. GDP/GTP cycling is regulated by large families of guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). ArfGEFs activate Arfs by mediating the exchange of GDP for GTP, while ArfGAPs terminate Arf function by stimulating the hydrolysis of the terminal phosphate group of GTP. Arf6 is a major regulator of endocytic trafficking and reorganization of the actin cytoskeleton in eukaryotic organisms. Owing to its participation in wide range of fundamentally distinct cellular processes, Arf6 may be a drug target for cancer and malaria amongst other diseases. As with cancer cells, rapid growth and viability of eukaryotic pathogens likely places a heavy burden on their endocytic pathways and a critical reliance on Arf6 activity. A putative malarial homolog of Arf6 (PfArf6) localises to numerous puncta along the periphery of the parasite in the mature trophozoite life stage of the parasite (T. Swart, MSc dissertation). Owing to highly inefficient parasite transfection procedures and a relative shortage of well described and validated parasite organelle markers, the possible functions of PfArf6 were explored using HeLa cells as a surrogate model for parasites by fluorescence microscopy of cells transfected with GFP-tagged PfArf6. Partial co-localisation was observed with the mammalian markers HsArf6 and LC3, which suggested possible roles in Arf6-dependent endocytosis and autophagy, respectively. While these possible roles are currently under investigation in parasites, an overall long-term goal which was initiated in this study was to determine whether PfArf6 is a valid drug target. To chemically validate PfArf6 as a drug target, a potent inhibitor needs to be identified. This requires the development of assays that may be employed for high-throughput screening of compound libraries. To support this goal, a novel plate-based assay was developed using human Arf6. The assay relies on the selective binding of an Arf effector protein domain (GGA3) fused to glutathione-S-transferase (GST), to His-tagged Arf6 immobilised on a nickel-coated plate. The assay format was developed and could robustly distinguish HsArf6-GDP (inactive) from HsArf6-GTP (active). Furthermore, it could be employed to detect the deactivation of Arf6 by ArfGAP1-stimualted GTP hydrolysis, but not Arf6 activation by ARNO-stimulated GDP/GTP exchange (ARNO is an ArfGEF). The ArfGAP1 deactivation assay was chemically validated using a known ArfGAP inhibitor, QS11. An improved assay was developed that employs JIP4 as an Arf6-specific binding partner instead of GGA3. In addition to superior performance, the alternative assay format could potentially be exploited for cancer drug discovery, since Arf6-JIP4 interaction has been implicated in cancer cell invasion and metastasis. Both assays may be employed to explore alternative ArfGEFs and ArfGAPs that act on Arf6 and contribute to the advancement of cancer. In parallel experiments, where development of PfArf6 assays was the focus, several issues arose. Firstly, we could not prepare GDP- and GTP-bound forms of PfArf6 since EDTA-mediated nucleotide exchange appeared to irreversibly destabilise the protein. However, PfArf6 activation (i.e. the preparation of PfArf6-GTP) was possible when mediated by ARNO and assessed by tryptophan fluorescence kinetic assays, suggesting that PfArf6 may be expressed in GDP-bound form in E. coli. As with human Arf6, ARNO-mediated GDP/GTP exchange on PfArf6 was not detectable in the immobilised PfArf6-GGA interaction GST assay format. However, a more sensitive assay was developed which relies on the use of nickel-horseradish peroxidase to detect the binding of His-tagged PfArf6 to JIP4-GST immobilised on glutathione plates and could detect ARNO-mediated PfArf6 activation. Since we could not prepare PfArf6-GTP (that did not rely on the presence of the ArfGEF, ARNO), malarial ArfGAP deactivation studies were conducted using PfArf1 instead of PfArf6 in the GGA-GST interaction assay. Both PfArfGAP1and PfArfGAP2 stimulated GTP hydrolysis by PfArf1, but only the former was inhibited by the standard human ArfGAP inhibitor, QS11. The development of these simple, cost-effective assays can be used in the high-throughput screening of novel anticancer and antimalarial compounds that target Arf signalling machineries. In theory, the assay could be extended as a tool to identify novel inhibitors of the multitude of Arfs, ArfGEFs and ArfGAPs originating from any organism and hence has broad clinical significance.
- Full Text:
- Date Issued: 2019