Characterization of the hydantoin-hydrolysing system of Pseudomonas putida RU-KM3s
- Authors: Matcher, Gwynneth Felicity
- Date: 2005
- Subjects: Hydantoin Hydrolysis Pseudomonas Enzymes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3939 , http://hdl.handle.net/10962/d1003998
- Description: The biocatalytic conversion of 5-monosubstituted hydantoin derivatives to optically pure amino acids involves two reaction steps: the hydrolysis of hydantoin to N-carbamylamino acid by an hydantoinase or dihydropyrimidinase enzyme, followed by conversion of the Ncarbamylamino acid to the corresponding amino acid by an N-carbamoylase enzyme. This biocatalytic process has been successfully applied in several industrial processes for the production of enantiomerically pure amino acids used in the synthesis of pharmaceuticals, insecticides, hormones, and food additives. P. putida RU-KM3S was selected for study based on inherent high levels of hydantoinase and N-carbamoylase activity. Subsequent biocatalytic analysis of the enzyme activity within this strain revealed unique properties thus prompting further characterization. The main focus of this research was the isolation of the genes encoding the hydantoin-hydrolysing pathway in RU-KM3S. A genomic library was constructed and screened for heterologous expression of the hydantoin-hydrolysing enzymes. However, this approach was unsuccessful prompting the use of transposon mutagenesis in order to circumvent the drawbacks associated with complementation studies. The enzymes responsible for hydantoin-hydrolysis were identified by insertional inactivation as a dihydropyrimidinase and b-ureidopropionase encoded by dhp and bup respectively. A third open reading frame, encoding a putative transport protein, was identified between the dhp and bup genes and appeared to share a promoter with bup. Analysis of the amino acid sequence deduced from bup and dhp substantiated the distinctive properties and potential industrial application of the L-enantioselective b-ureidopropionase and provided targets for potential optimisation of the substrate-selectivity and activity of the dihydropyrimidinase by site directed mutagenesis. Several transposon-generated mutants with an altered phenotype for growth on minimal medium with hydantoin as the sole source of nitrogen were also isolated. Analysis of the insertion events in these mutants revealed disruptions of genes encoding key elements of the Ntr global regulatory pathway. However, inactivation of these genes had no effect on the dihydropyrimidinase and b-ureidopropionase activity levels. An additional mutant in which the gene coding for the dihydrolipoamide succinyltransferase, which is involved in the TCA cycle, was isolated with reduced levels of both dihydropyrimidinase and b-ureidopropionase activities. These results indicated that the hydantoin-hydrolysis pathway in RU-KM3S is regulated by carbon rather than nitrogen catabolite repression. This was confirmed by the reduction of hydantoin-hydrolysis in cells grown in excess carbon as opposed to nitrogen. Identification of a putative CRP-binding site within the promoter region of these enzymes further supported the regulatory role of carbon catabolite repression (CCR). As CCR in Pseudomonads is poorly understood, elucidation of the mechanism by which the hydantoinhydrolysing pathway in RU-KM3S is regulated would provide valuable insight into this complex process.
- Full Text:
- Date Issued: 2005
- Authors: Matcher, Gwynneth Felicity
- Date: 2005
- Subjects: Hydantoin Hydrolysis Pseudomonas Enzymes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3939 , http://hdl.handle.net/10962/d1003998
- Description: The biocatalytic conversion of 5-monosubstituted hydantoin derivatives to optically pure amino acids involves two reaction steps: the hydrolysis of hydantoin to N-carbamylamino acid by an hydantoinase or dihydropyrimidinase enzyme, followed by conversion of the Ncarbamylamino acid to the corresponding amino acid by an N-carbamoylase enzyme. This biocatalytic process has been successfully applied in several industrial processes for the production of enantiomerically pure amino acids used in the synthesis of pharmaceuticals, insecticides, hormones, and food additives. P. putida RU-KM3S was selected for study based on inherent high levels of hydantoinase and N-carbamoylase activity. Subsequent biocatalytic analysis of the enzyme activity within this strain revealed unique properties thus prompting further characterization. The main focus of this research was the isolation of the genes encoding the hydantoin-hydrolysing pathway in RU-KM3S. A genomic library was constructed and screened for heterologous expression of the hydantoin-hydrolysing enzymes. However, this approach was unsuccessful prompting the use of transposon mutagenesis in order to circumvent the drawbacks associated with complementation studies. The enzymes responsible for hydantoin-hydrolysis were identified by insertional inactivation as a dihydropyrimidinase and b-ureidopropionase encoded by dhp and bup respectively. A third open reading frame, encoding a putative transport protein, was identified between the dhp and bup genes and appeared to share a promoter with bup. Analysis of the amino acid sequence deduced from bup and dhp substantiated the distinctive properties and potential industrial application of the L-enantioselective b-ureidopropionase and provided targets for potential optimisation of the substrate-selectivity and activity of the dihydropyrimidinase by site directed mutagenesis. Several transposon-generated mutants with an altered phenotype for growth on minimal medium with hydantoin as the sole source of nitrogen were also isolated. Analysis of the insertion events in these mutants revealed disruptions of genes encoding key elements of the Ntr global regulatory pathway. However, inactivation of these genes had no effect on the dihydropyrimidinase and b-ureidopropionase activity levels. An additional mutant in which the gene coding for the dihydrolipoamide succinyltransferase, which is involved in the TCA cycle, was isolated with reduced levels of both dihydropyrimidinase and b-ureidopropionase activities. These results indicated that the hydantoin-hydrolysis pathway in RU-KM3S is regulated by carbon rather than nitrogen catabolite repression. This was confirmed by the reduction of hydantoin-hydrolysis in cells grown in excess carbon as opposed to nitrogen. Identification of a putative CRP-binding site within the promoter region of these enzymes further supported the regulatory role of carbon catabolite repression (CCR). As CCR in Pseudomonads is poorly understood, elucidation of the mechanism by which the hydantoinhydrolysing pathway in RU-KM3S is regulated would provide valuable insight into this complex process.
- Full Text:
- Date Issued: 2005
Using aspects of game theory for enhanced stakeholder participation perspectives in integrated water resource management: a Kat River Valley case study
- Authors: Mbatha, Cyril
- Date: 2005
- Subjects: Water resources development -- Case studies , Water-supply -- South Africa -- Kat River Valley -- Management , Economic development -- Case studies , Water-supply -- Government policy -- South Africa , Economic development -- South Africa -- Kat River Valley , Game theory
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:971 , http://hdl.handle.net/10962/d1002705 , Water resources development -- Case studies , Water-supply -- South Africa -- Kat River Valley -- Management , Economic development -- Case studies , Water-supply -- Government policy -- South Africa , Economic development -- South Africa -- Kat River Valley , Game theory
- Description: South Africa is a water poor region and yet, like in many other developing countries, water resources are a valuable production input in socially important industries such as agriculture and mining (Nieuwoudt et al., 2004:162-182). With a mean annual rainfall and runoff of 502mm and 42mm (420m³ per hectare per annum) respectively, Briers and Powell (1993:1) speculate that water shortages will limit South Africa’s economic development in the twenty-first century. In response to the challenges of water resource scarcity and socio-economic inequalities, in 1997 the South African Department of Water Affairs and Forestry (DWAF), following international trends, formulated a National Water Policy (NWP) based on Integrated Water Resource Management (IWRM) principles (Palmer et al., 2003). The main objectives of the NWP (1997) are to protect national water resources from degradation, provide economically disadvantaged communities with access to water resources and promote economic development, through the devolution of water management responsibilities to locally established water institutions (NWP, 1997). The pursuit of these objectives requires a thorough investigation of biophysical, sociopolitical and economic characteristics of the demarcated water areas. For such an investigation to provide accurate and locally relevant results, broad stakeholder participation is of paramount importance. The thesis discusses economic measures required in pursuing enhanced stakeholder participation levels at local levels. A socio-economic survey investigation describing major participation trends against reported property rights and Willingness to Pay values was conducted in the Kat River Valley, Eastern Cape province. From the investigation, observed participation trends driven by economic interests were identified and used in recommendations proposed for the policy implementation process. Using game theoretic arguments as guidelines in soliciting cooperative behaviour in the use and consumption of common resources (Ostrom, 1990), the thesis proposes the introduction of water related public works projects as employment creating vehicles for individuals and communities targeted for economic empowerment and participation in the water policy. However, for sustainable stakeholder participation levels, it is argued that the employment positions created through the projects need to be designed in a manner that would encourage an evolution of long-term relationships between stakeholders and the water management institutions.
- Full Text:
- Date Issued: 2005
- Authors: Mbatha, Cyril
- Date: 2005
- Subjects: Water resources development -- Case studies , Water-supply -- South Africa -- Kat River Valley -- Management , Economic development -- Case studies , Water-supply -- Government policy -- South Africa , Economic development -- South Africa -- Kat River Valley , Game theory
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:971 , http://hdl.handle.net/10962/d1002705 , Water resources development -- Case studies , Water-supply -- South Africa -- Kat River Valley -- Management , Economic development -- Case studies , Water-supply -- Government policy -- South Africa , Economic development -- South Africa -- Kat River Valley , Game theory
- Description: South Africa is a water poor region and yet, like in many other developing countries, water resources are a valuable production input in socially important industries such as agriculture and mining (Nieuwoudt et al., 2004:162-182). With a mean annual rainfall and runoff of 502mm and 42mm (420m³ per hectare per annum) respectively, Briers and Powell (1993:1) speculate that water shortages will limit South Africa’s economic development in the twenty-first century. In response to the challenges of water resource scarcity and socio-economic inequalities, in 1997 the South African Department of Water Affairs and Forestry (DWAF), following international trends, formulated a National Water Policy (NWP) based on Integrated Water Resource Management (IWRM) principles (Palmer et al., 2003). The main objectives of the NWP (1997) are to protect national water resources from degradation, provide economically disadvantaged communities with access to water resources and promote economic development, through the devolution of water management responsibilities to locally established water institutions (NWP, 1997). The pursuit of these objectives requires a thorough investigation of biophysical, sociopolitical and economic characteristics of the demarcated water areas. For such an investigation to provide accurate and locally relevant results, broad stakeholder participation is of paramount importance. The thesis discusses economic measures required in pursuing enhanced stakeholder participation levels at local levels. A socio-economic survey investigation describing major participation trends against reported property rights and Willingness to Pay values was conducted in the Kat River Valley, Eastern Cape province. From the investigation, observed participation trends driven by economic interests were identified and used in recommendations proposed for the policy implementation process. Using game theoretic arguments as guidelines in soliciting cooperative behaviour in the use and consumption of common resources (Ostrom, 1990), the thesis proposes the introduction of water related public works projects as employment creating vehicles for individuals and communities targeted for economic empowerment and participation in the water policy. However, for sustainable stakeholder participation levels, it is argued that the employment positions created through the projects need to be designed in a manner that would encourage an evolution of long-term relationships between stakeholders and the water management institutions.
- Full Text:
- Date Issued: 2005
The perceived impact of policy change on leadership and management : a case study of a Namibian school
- Moelanjane, Porgarius Porgacy Rukee
- Authors: Moelanjane, Porgarius Porgacy Rukee
- Date: 2005
- Subjects: School management and organization -- Namibia -- Case studies. Educational leadership -- Namibia -- Case studies Educational change -- Namibia -- Case studies Education and state -- Namibia -- Case studies
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1935 , http://hdl.handle.net/10962/d1007670
- Description: The transformation of education has been central to the programme of the first democratically elected government in Namibia, both because of the democratic demands for equality, equity and access, and as a means towards the wider transformation of Namibian community. The study provides a brief history of the pre- and post-independence education policy environment in Namibia with regard to how management and leadership of schools gave rise to opportunities for the development of democratic participation. Adopting an interpretive approach, the study examines several key stakeholders' perceptions of the impact of transformation initiatives on their leadership and management thinking and practice. The sample comprised four teachers, a principal, a director, a school board member, two learners and a hostel matron. The findings reveal that, in order to create a democratic school community, the stakeholders' role is largely a matter of opposing and transcending the contradictions, inadequacies and limitations inherent in the educational ideas, policies and practices caused by the previous Apartheid education system. There is a reasonable amount of success in the implementation process of democratic leadership and management of schools. The findings further highlight contradictory tendencies, in that the Ministry of Basic Education Sports and Culture established a uniform system of education but also entertained the principle of decentralised governance through the introduction of school based management practice incorporating the main stakeholders at local level. The data confirms a communication gap between stakeholders, lack of adequate education and training in participatory management and leadership for learners, parents, teachers and school boards, and insufficient support from the national and the regional authorities. The study concludes with some specific recommendations that might strengthen stakeholders' sense of their participation in democratic practices for longer-term cost effective implementation to occur. This is supported by the belief that education policy change only becomes a reality once understood, owned and appreciated at local level.
- Full Text:
- Date Issued: 2005
- Authors: Moelanjane, Porgarius Porgacy Rukee
- Date: 2005
- Subjects: School management and organization -- Namibia -- Case studies. Educational leadership -- Namibia -- Case studies Educational change -- Namibia -- Case studies Education and state -- Namibia -- Case studies
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1935 , http://hdl.handle.net/10962/d1007670
- Description: The transformation of education has been central to the programme of the first democratically elected government in Namibia, both because of the democratic demands for equality, equity and access, and as a means towards the wider transformation of Namibian community. The study provides a brief history of the pre- and post-independence education policy environment in Namibia with regard to how management and leadership of schools gave rise to opportunities for the development of democratic participation. Adopting an interpretive approach, the study examines several key stakeholders' perceptions of the impact of transformation initiatives on their leadership and management thinking and practice. The sample comprised four teachers, a principal, a director, a school board member, two learners and a hostel matron. The findings reveal that, in order to create a democratic school community, the stakeholders' role is largely a matter of opposing and transcending the contradictions, inadequacies and limitations inherent in the educational ideas, policies and practices caused by the previous Apartheid education system. There is a reasonable amount of success in the implementation process of democratic leadership and management of schools. The findings further highlight contradictory tendencies, in that the Ministry of Basic Education Sports and Culture established a uniform system of education but also entertained the principle of decentralised governance through the introduction of school based management practice incorporating the main stakeholders at local level. The data confirms a communication gap between stakeholders, lack of adequate education and training in participatory management and leadership for learners, parents, teachers and school boards, and insufficient support from the national and the regional authorities. The study concludes with some specific recommendations that might strengthen stakeholders' sense of their participation in democratic practices for longer-term cost effective implementation to occur. This is supported by the belief that education policy change only becomes a reality once understood, owned and appreciated at local level.
- Full Text:
- Date Issued: 2005
The assessment and management of bycatch and discards in the South African demersal trawl fishery
- Authors: Walmsley, Sarah Ann
- Date: 2005
- Subjects: Bycatches (Fisheries) Fishery management -- South Africa Fisheries -- South Africa Trawls and trawling -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5228 , http://hdl.handle.net/10962/d1005071
- Description: Over the past few decades it has become recognised that an ecosystem approach is required to manage world fisheries. Management strategies must ensure that non-target (bycatch) as well as target catches are sustainable. To achieve this, detailed commercial catch and biological information is required. The composition of catches made by trawlers operating off the south and west coasts of South Africa was investigated. Distinct fishing areas were identified on each coast, based on target species and fishing depth. Catch composition differed markedly among the areas defined. Although hake Merluccius sp. dominated South Coast catches, a large proportion of the catch was composed of bycatch. On the West Coast, hake dominated catches and this domination increased with increasing depth. On both coasts approximately 90% of the observed nominal catch was processed and landed. Estimates of annual discards suggested that the fishery discarded 38 thousand tons of fish per annum (16% of the nominal trawl catch). The data also indicated that hake discarding, the capture of linefish and the increased targeting of high value species might be cause for concern. Spatial analysis indicated that a variety of factors such as trawling position, catch size and catch composition affects bycatch dynamics. The monkfish Lophius vomerinus is a common bycatch species that has been increasingly targeted by demersal trawlers. This study showed that L. vomerinus is a slow-growing, long-lived species (West Coast males L∞ = 68.50cm TL, t₀ = -1.69yr, K = 0.10yr⁻¹; West Coast females L∞ = 110.23cm TL, t₀ = -1.54yr, K = 0.05yr⁻¹; South Coast sexes combined L∞ = 70.12cm TL, t₀ = - 0.80yr, K = 0.11yr⁻¹), that matures at approximately 6 years of age. These traits could have serious management implications for the species. Per-recruit analysis suggested that the stock might be overexploited, although further investigation is required to confirm this. Solutions were suggested for each of the concerns raised, taking cognisance of the differences observed between the South and West Coasts and the economic dependence of South Coast companies on bycatch. The needs of future research were considered.
- Full Text:
- Date Issued: 2005
- Authors: Walmsley, Sarah Ann
- Date: 2005
- Subjects: Bycatches (Fisheries) Fishery management -- South Africa Fisheries -- South Africa Trawls and trawling -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5228 , http://hdl.handle.net/10962/d1005071
- Description: Over the past few decades it has become recognised that an ecosystem approach is required to manage world fisheries. Management strategies must ensure that non-target (bycatch) as well as target catches are sustainable. To achieve this, detailed commercial catch and biological information is required. The composition of catches made by trawlers operating off the south and west coasts of South Africa was investigated. Distinct fishing areas were identified on each coast, based on target species and fishing depth. Catch composition differed markedly among the areas defined. Although hake Merluccius sp. dominated South Coast catches, a large proportion of the catch was composed of bycatch. On the West Coast, hake dominated catches and this domination increased with increasing depth. On both coasts approximately 90% of the observed nominal catch was processed and landed. Estimates of annual discards suggested that the fishery discarded 38 thousand tons of fish per annum (16% of the nominal trawl catch). The data also indicated that hake discarding, the capture of linefish and the increased targeting of high value species might be cause for concern. Spatial analysis indicated that a variety of factors such as trawling position, catch size and catch composition affects bycatch dynamics. The monkfish Lophius vomerinus is a common bycatch species that has been increasingly targeted by demersal trawlers. This study showed that L. vomerinus is a slow-growing, long-lived species (West Coast males L∞ = 68.50cm TL, t₀ = -1.69yr, K = 0.10yr⁻¹; West Coast females L∞ = 110.23cm TL, t₀ = -1.54yr, K = 0.05yr⁻¹; South Coast sexes combined L∞ = 70.12cm TL, t₀ = - 0.80yr, K = 0.11yr⁻¹), that matures at approximately 6 years of age. These traits could have serious management implications for the species. Per-recruit analysis suggested that the stock might be overexploited, although further investigation is required to confirm this. Solutions were suggested for each of the concerns raised, taking cognisance of the differences observed between the South and West Coasts and the economic dependence of South Coast companies on bycatch. The needs of future research were considered.
- Full Text:
- Date Issued: 2005
An investigation of local community contributions to the Malawi College of Fisheries curriculum: a case study focussing on the Chambo fishery
- Kachilonda, Dick Daffu Kachanga
- Authors: Kachilonda, Dick Daffu Kachanga
- Date: 2005
- Subjects: Malawi College of Fisheries -- Curricula Fishery management -- Study and teaching Fishery management -- Malawi Fisheries -- Malawi Fishes -- Nyasa, Lake
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1857 , http://hdl.handle.net/10962/d1004537
- Description: The aim of the study was to investigate local community contributions to the existing Malawi College of Fisheries curriculum with a focus on Chambo fishery. Chambo fish (Oreochromis species) is the most favoured fish in Lake Malawi. Chambo catches have declined over the years. Responding to the declining catches, the Fisheries Department is engaged in a number of management options to address the issues. Most of these management options are governed by scientific recommendations and do not consider the socio-economic situation of the people who are dependent on fishery. This approach to fishery has influenced the Malawi College of Fisheries curriculum. The existing curriculum is product-centred, developed by a consultant. During the development of the curriculum, there was little consultation with the lecturers and no consultation with the local communities who are using the resource. The fishing communities have been fishing for a long time and have acquired knowledge, skills and experience worth investigating for its potential role in improving the existing curriculum. Through the use of interviews, focus group discussions and workshops with local communities local knowledge was identified for inclusion into the curriculum. A review of the existing curriculum revealed that it has primarily technical focus, grounded in the protection, control and management of the fish stocks, while the local knowledge has a practical focus based on existing practices and requiring an understanding of the sources of the issues. There is also more emphasis on a historical perspective and the context in which fishing practices take place at the moment. It was evident from the study that local communities have much of knowledge, skills and experience gained over the years of fishing , and if properly utilised, it can improve the MCF curriculum. I therefore recommend in this study that the curriculum be reviewed in order to integrate and draw on the local knowledge through a deliberative and participatory process between the local communities and the government so that it addresses the needs of the local communities and improves the training of the extension workers.
- Full Text:
- Date Issued: 2005
- Authors: Kachilonda, Dick Daffu Kachanga
- Date: 2005
- Subjects: Malawi College of Fisheries -- Curricula Fishery management -- Study and teaching Fishery management -- Malawi Fisheries -- Malawi Fishes -- Nyasa, Lake
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1857 , http://hdl.handle.net/10962/d1004537
- Description: The aim of the study was to investigate local community contributions to the existing Malawi College of Fisheries curriculum with a focus on Chambo fishery. Chambo fish (Oreochromis species) is the most favoured fish in Lake Malawi. Chambo catches have declined over the years. Responding to the declining catches, the Fisheries Department is engaged in a number of management options to address the issues. Most of these management options are governed by scientific recommendations and do not consider the socio-economic situation of the people who are dependent on fishery. This approach to fishery has influenced the Malawi College of Fisheries curriculum. The existing curriculum is product-centred, developed by a consultant. During the development of the curriculum, there was little consultation with the lecturers and no consultation with the local communities who are using the resource. The fishing communities have been fishing for a long time and have acquired knowledge, skills and experience worth investigating for its potential role in improving the existing curriculum. Through the use of interviews, focus group discussions and workshops with local communities local knowledge was identified for inclusion into the curriculum. A review of the existing curriculum revealed that it has primarily technical focus, grounded in the protection, control and management of the fish stocks, while the local knowledge has a practical focus based on existing practices and requiring an understanding of the sources of the issues. There is also more emphasis on a historical perspective and the context in which fishing practices take place at the moment. It was evident from the study that local communities have much of knowledge, skills and experience gained over the years of fishing , and if properly utilised, it can improve the MCF curriculum. I therefore recommend in this study that the curriculum be reviewed in order to integrate and draw on the local knowledge through a deliberative and participatory process between the local communities and the government so that it addresses the needs of the local communities and improves the training of the extension workers.
- Full Text:
- Date Issued: 2005
Bioactive 4-methoxypyrrolic natural products from two South African marine invertebrates
- Authors: Rapson, Trevor Douglas
- Date: 2005
- Subjects: Natural products -- South Africa , Marine invertebrates -- South Africa , Bryozoa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4412 , http://hdl.handle.net/10962/d1006766 , Natural products -- South Africa , Marine invertebrates -- South Africa , Bryozoa
- Description: This thesis presents an investigation of the 4-methoxypyrrolic constituents of two South African marine invertebrates, the nudibranch Tambja capensis and the bryozoan Bugula dentata. Three known compounds tambjamine A (7), tambjamine E (13) and the tetrapyrrole (15) were isolated during this investigation. All three compounds were shown to be active against oesophageal cancer in accordance with the general anticancer and immunosuppressive properties observed for 4-methoxypyrrolic natural products. Tambjamine A (7), tambjamine E (13) and the tetrapyrrole (15), together with tambjamine K (21) and L (22) (previously isolated in our laboratory) were used as standards to quantitatively assess the presence of these tambjamines in T. capensis and B. dentata collected from three different sites along the South African coast. This study confirmed that B. dentata is the source of the 4-methoxypyrrolic natural products sequestered by T. capensis and eliminated the closely related bryozoan B. neritina as a source of these metabolites. The paucity of tambjamine L (21) and K (22) obtained in previous investigations of the sequestered chemistry of T. capensis prompted an attempt at the development of synthetic methodology that could be used to synthesize tambjamines in sufficient yield for in depth bioactivity studies. In order to by pass the extensively reported problems associated with the synthesis of this group of compound 3-methoxy-2-formylpyrrole (47), readily accessible from 3-methoxypyridine N-oxide (48), was used as the starting material in a singlet oxygen induced 2,2’ bipyrrole coupling reaction. Although 47 proved unreactive in this coupling reaction, when the N-Boc protected analogue of 47 was used, and the reaction worked up in the dark, the novel methyl 4-aza-5-oxo-6,6-di-(2-pyrrolyl)-2(Z)-hexenoate (57) was obtained in low yield. The physical properties of tambjamine (E) (13) and the tetrapyrrole (15) were investigated to further the understanding of the proposed oxidative DNA cleavage mechanism and to determine the potential of the 4-methoxypyrrolic natural products as photodynamic therapy agents.
- Full Text:
- Date Issued: 2005
- Authors: Rapson, Trevor Douglas
- Date: 2005
- Subjects: Natural products -- South Africa , Marine invertebrates -- South Africa , Bryozoa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4412 , http://hdl.handle.net/10962/d1006766 , Natural products -- South Africa , Marine invertebrates -- South Africa , Bryozoa
- Description: This thesis presents an investigation of the 4-methoxypyrrolic constituents of two South African marine invertebrates, the nudibranch Tambja capensis and the bryozoan Bugula dentata. Three known compounds tambjamine A (7), tambjamine E (13) and the tetrapyrrole (15) were isolated during this investigation. All three compounds were shown to be active against oesophageal cancer in accordance with the general anticancer and immunosuppressive properties observed for 4-methoxypyrrolic natural products. Tambjamine A (7), tambjamine E (13) and the tetrapyrrole (15), together with tambjamine K (21) and L (22) (previously isolated in our laboratory) were used as standards to quantitatively assess the presence of these tambjamines in T. capensis and B. dentata collected from three different sites along the South African coast. This study confirmed that B. dentata is the source of the 4-methoxypyrrolic natural products sequestered by T. capensis and eliminated the closely related bryozoan B. neritina as a source of these metabolites. The paucity of tambjamine L (21) and K (22) obtained in previous investigations of the sequestered chemistry of T. capensis prompted an attempt at the development of synthetic methodology that could be used to synthesize tambjamines in sufficient yield for in depth bioactivity studies. In order to by pass the extensively reported problems associated with the synthesis of this group of compound 3-methoxy-2-formylpyrrole (47), readily accessible from 3-methoxypyridine N-oxide (48), was used as the starting material in a singlet oxygen induced 2,2’ bipyrrole coupling reaction. Although 47 proved unreactive in this coupling reaction, when the N-Boc protected analogue of 47 was used, and the reaction worked up in the dark, the novel methyl 4-aza-5-oxo-6,6-di-(2-pyrrolyl)-2(Z)-hexenoate (57) was obtained in low yield. The physical properties of tambjamine (E) (13) and the tetrapyrrole (15) were investigated to further the understanding of the proposed oxidative DNA cleavage mechanism and to determine the potential of the 4-methoxypyrrolic natural products as photodynamic therapy agents.
- Full Text:
- Date Issued: 2005
The refinement of protective salinity guidelines for South African freshwater resources
- Authors: Slaughter, Andrew Robert
- Date: 2005
- Subjects: Water salinization -- South Africa Salinization -- Control -- South Africa Salinity -- South Africa Freshwater ecology -- South Africa Water resources development -- South Africa Caridina Caridina -- Effect of pollution
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6031 , http://hdl.handle.net/10962/d1006170
- Description: South Africa is an arid country and its growing population is putting freshwater resources under increasing pressure. Natural salinization of freshwater systems is being exacerbated by anthropogenic influences. The National Water Act (No. 36 of 1998) stipulates the need for an ecological Reserve, that quantity and quality of freshwater needed to protect freshwater ecosystems while allowing sustainable use of freshwater resources. Water guidelines do exist in the form of the South African Water Quality Guidelines (DWAF, 1996) and more recently, Jooste and Rossouw (2002) compiled benchmark values for water quality variables marking the boundaries between ecological health classes in the 4-category classification system. Predominantly international toxicity data were used to compile the guidelines and the benchmark values. In addition, there is a paucity of chronic toxicity data nationally and internationally. This thesis showed that it is statistically possible to derive protective chronic endpoints for salinity from acute toxicity data through extrapolation. The Acute to Chronic Ratio (ACR), Two-Step Linear Regression (LRA) and Multi-Factor Probit Analysis (MPA) extrapolation methods were investigated to derive chronic toxicity data from acute toxicity data. The authors of LRA and MPA recommend associating a time independent LCx value in the range of LC₀¸₀₁ to LC₁₀ with a Predicted No Effect Concentration (PNOEC). In addition to published methods, this thesis studied the possibility of equating a time independent LC₅₀ value and subjected to a safety factor of 5 (LRA LC₅₀/5), to the PNOEC. Extrapolated chronic toxicity data where the toxicants are NaCl and Na₂SO₄ were derived for indigenous South African macroinvertebrates. NaCl and Na₂SO₄ are salts associated with salinisation in South Africa. In addition, a chronic salinity toxicity test protocol for an indigenous South African aquatic macroinvertebrate was designed and chronic toxicity test were performed using NaCl and Na₂SO₄ as toxicants. The experimental chronic toxicity data produced were used to validate results from the acute to chronic extrapolation methods. Extrapolated chronic toxicity data were inputted into Species Sensitivity Distribution curves, and concentrations that were predicted to protect 95 % of species (PC95) were compared to the sub-lethality benchmarks proposed by Jooste and Rossouw (2002) for NaCl and Na₂SO₄. This study concluded that the LRA LC₅₀/5 extrapolation method is the most protective and accurate and proposed that LRA replace the ACR method in future guideline development for inorganic salts.
- Full Text:
- Date Issued: 2005
- Authors: Slaughter, Andrew Robert
- Date: 2005
- Subjects: Water salinization -- South Africa Salinization -- Control -- South Africa Salinity -- South Africa Freshwater ecology -- South Africa Water resources development -- South Africa Caridina Caridina -- Effect of pollution
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6031 , http://hdl.handle.net/10962/d1006170
- Description: South Africa is an arid country and its growing population is putting freshwater resources under increasing pressure. Natural salinization of freshwater systems is being exacerbated by anthropogenic influences. The National Water Act (No. 36 of 1998) stipulates the need for an ecological Reserve, that quantity and quality of freshwater needed to protect freshwater ecosystems while allowing sustainable use of freshwater resources. Water guidelines do exist in the form of the South African Water Quality Guidelines (DWAF, 1996) and more recently, Jooste and Rossouw (2002) compiled benchmark values for water quality variables marking the boundaries between ecological health classes in the 4-category classification system. Predominantly international toxicity data were used to compile the guidelines and the benchmark values. In addition, there is a paucity of chronic toxicity data nationally and internationally. This thesis showed that it is statistically possible to derive protective chronic endpoints for salinity from acute toxicity data through extrapolation. The Acute to Chronic Ratio (ACR), Two-Step Linear Regression (LRA) and Multi-Factor Probit Analysis (MPA) extrapolation methods were investigated to derive chronic toxicity data from acute toxicity data. The authors of LRA and MPA recommend associating a time independent LCx value in the range of LC₀¸₀₁ to LC₁₀ with a Predicted No Effect Concentration (PNOEC). In addition to published methods, this thesis studied the possibility of equating a time independent LC₅₀ value and subjected to a safety factor of 5 (LRA LC₅₀/5), to the PNOEC. Extrapolated chronic toxicity data where the toxicants are NaCl and Na₂SO₄ were derived for indigenous South African macroinvertebrates. NaCl and Na₂SO₄ are salts associated with salinisation in South Africa. In addition, a chronic salinity toxicity test protocol for an indigenous South African aquatic macroinvertebrate was designed and chronic toxicity test were performed using NaCl and Na₂SO₄ as toxicants. The experimental chronic toxicity data produced were used to validate results from the acute to chronic extrapolation methods. Extrapolated chronic toxicity data were inputted into Species Sensitivity Distribution curves, and concentrations that were predicted to protect 95 % of species (PC95) were compared to the sub-lethality benchmarks proposed by Jooste and Rossouw (2002) for NaCl and Na₂SO₄. This study concluded that the LRA LC₅₀/5 extrapolation method is the most protective and accurate and proposed that LRA replace the ACR method in future guideline development for inorganic salts.
- Full Text:
- Date Issued: 2005
"Feeling foggy?": an investigation into the self-reported post-concussive symptoms in rugby union players at university level
- Authors: Boulind, Melissa
- Date: 2005
- Subjects: Brain damage , Brain -- Wounds and injuries -- Complications , Brain -- Wounds and injuries -- Psychology , Sports injuries -- Psychological aspects , Rugby football injuries , Rugby Union football players
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2938 , http://hdl.handle.net/10962/d1002447 , Brain damage , Brain -- Wounds and injuries -- Complications , Brain -- Wounds and injuries -- Psychology , Sports injuries -- Psychological aspects , Rugby football injuries , Rugby Union football players
- Description: A study was conducted on the self-reported symptoms of Mild Traumatic Brain Injury sustained in Rugby Union at the pre- and post-season stages. A full sample of 30 rugby players at Rhodes University was compared to 27 non-contact sport controls. A reduced sample of 20 rugby players and 9 control participants provided improved control for education and IQ and was compared. Measures included the WAIS-III Vocabulary and Picture Completion Sub-tests to estimate IQ level, the symptom checklist on a widely used computer-based program (ImPACT), and a paper and pencil self-report 31-Item Post-Concussion Symptom Questionnaire. Independent and Dependent T-Test comparisons were conducted on the full and reduced samples. The symptoms reported by the rugby group appeared to be more pronounced on both the ImPACT Symptom Scale and the 31-Item Post-Concussion Symptom Questionnaire when compared to the control group at both the pre-and post-season stages. It was concluded that the rugby players demonstrated evidence to support the hypothesis of having sustained more previous concussions and reporting more symptoms at the pre-season stage when compared to comtrol participants. No prevalent changes for either the rugby or control groups were seen in dependent comparisons from pre-to post-season.
- Full Text:
- Date Issued: 2005
- Authors: Boulind, Melissa
- Date: 2005
- Subjects: Brain damage , Brain -- Wounds and injuries -- Complications , Brain -- Wounds and injuries -- Psychology , Sports injuries -- Psychological aspects , Rugby football injuries , Rugby Union football players
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2938 , http://hdl.handle.net/10962/d1002447 , Brain damage , Brain -- Wounds and injuries -- Complications , Brain -- Wounds and injuries -- Psychology , Sports injuries -- Psychological aspects , Rugby football injuries , Rugby Union football players
- Description: A study was conducted on the self-reported symptoms of Mild Traumatic Brain Injury sustained in Rugby Union at the pre- and post-season stages. A full sample of 30 rugby players at Rhodes University was compared to 27 non-contact sport controls. A reduced sample of 20 rugby players and 9 control participants provided improved control for education and IQ and was compared. Measures included the WAIS-III Vocabulary and Picture Completion Sub-tests to estimate IQ level, the symptom checklist on a widely used computer-based program (ImPACT), and a paper and pencil self-report 31-Item Post-Concussion Symptom Questionnaire. Independent and Dependent T-Test comparisons were conducted on the full and reduced samples. The symptoms reported by the rugby group appeared to be more pronounced on both the ImPACT Symptom Scale and the 31-Item Post-Concussion Symptom Questionnaire when compared to the control group at both the pre-and post-season stages. It was concluded that the rugby players demonstrated evidence to support the hypothesis of having sustained more previous concussions and reporting more symptoms at the pre-season stage when compared to comtrol participants. No prevalent changes for either the rugby or control groups were seen in dependent comparisons from pre-to post-season.
- Full Text:
- Date Issued: 2005
Euphausiid population structure and grazing in the Indian sector of the Antarctic Polar Frontal Zone, during austral autumn
- Bernard, Anthony Thomas Firth
- Authors: Bernard, Anthony Thomas Firth
- Date: 2005
- Subjects: Euphausiacea -- Antarctic Ocean , Euphausiacea -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5913 , http://hdl.handle.net/10962/d1015960
- Description: The trophodynamics of the numerically dominant euphausiid species within a region of high mesoscale oceanographic variability in the southwest Indian sector of the Antarctic Polar Frontal Zone (PFZ) were investigated during the austral autumns April/May) of 2004 and 2005. During the 2004 survey, sub-surface (200 m) temperature profiles indicated that an intense frontal feature, formed by the convergence of the Sub-Antarctic Front (SAF) and the Antarctic Polar Front (APF) bisected the survey area into two distinct zones, the Sub- Antarctic Zone (SAZ) and the Antarctic Zone (AAZ). Total integrated chlorophyll-a (chl-a) biomass was typical for the region (< 25 mg chl-a m⁻²), and was dominated by picophytoplankton. Total euphausiid abundance and biomass ranged from 0.1 to 3.1 ind m⁻³ and 0.1 to 8.1 mg dwt m⁻³, respectively, and did not differ significantly between the stations occupied in the SAZ and AAZ (p > 0.05). The multivariate analysis identified two interacting mechanisms controlling the distribution patterns, abundance and biomass of the various euphausiid species, namely (1) diel changes in abundance and biomass, (2) and restricted distribution patterns associated with the different water masses. Ingestion rates were determined for five euphausiid species. E. triacantha was found to have the highest daily ingestion rate ranging from 1 226.1 to 6 029.1 ng pigm ind⁻¹d⁻¹, while the lowest daily ingestion rates were observed in the juvenile Thysanoessa species (6.4 to 943.0 ng pigm ind⁻¹ d⁻¹). The total grazing impact of the selected euphausiids ranged from < 0.1 to 20.1 μg pigm m⁻²d⁻¹, corresponding to < 0.15 % of the areal chl-a biomass. The daily ration estimates of autotrophic carbon for the euphausiids suggested that phytoplankton represented a minor component in their diets, with only the sub-adult E. vallentini consuming sufficient phytoplankton to meet their daily carbon requirements. A cyclonic cold-core eddy spawned from the region of the APF located in the southwest Indian sector of the PFZ was the dominant feature during the 2005 survey. The total areal chl-a biomass throughout the region was low, ranging between 5.6 and 11.4 mg chl-a m⁻², and was significantly higher within the core of the eddy compared to the surrounding waters (p < 0.05). RMT-8 and WP-2 total euphausiid abundance and biomass estimates were high, and ranged from 0.004 to 0.36 ind m⁻³ and 0.065 to 1.21 mg dwt m⁻³, and from 0.01 to 18.2 ind m⁻³ and 0.01 to 15.7 mg dwt m⁻³, respectively. A distinct spatial pattern in the euphausiid community was evident with the Antarctic species, Euphausia frigida, E. triacantha and E. superba predominating within the core of the eddy, while the PFZ waters were characterized by the sub-Antarctic species, E. longirostris, Stylocheiron maximum, Nematoscelis megalops and Thysanoessa gregaria. The eddy edge acted as a transition zone where species from both regions co-occurred. Within the survey area the combined ingestion rate of the six numerically dominant euphausiid species ranged between 0.02 and 5.31 μg pigm m⁻²d¹, which corresponded to a loss of between < 0.001 and 0.11 % of the available chl-a biomass. E. triacantha and juvenile T. macura were identified as the dominant grazers. There was no apparent spatial pattern in the grazing activity of the euphausiids within the region of investigation. The average daily rations of the euphausiids examined were < 2 % of their body carbon. The low daily ration of the euphausiids could be ascribed to the predominance of small picophytoplankton in the region of investigation, which are too small to be grazed efficiently by larger zooplankton. The marked spatial patterns in species composition and the elevated abundance and biomass of euphausiids, suggest that the mesoscale eddies contribute to the spatial and temporal heterogeneity of the planktonic community of the PFZ and may represent important foraging regions for many of the apex predators within the region.
- Full Text:
- Date Issued: 2005
- Authors: Bernard, Anthony Thomas Firth
- Date: 2005
- Subjects: Euphausiacea -- Antarctic Ocean , Euphausiacea -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5913 , http://hdl.handle.net/10962/d1015960
- Description: The trophodynamics of the numerically dominant euphausiid species within a region of high mesoscale oceanographic variability in the southwest Indian sector of the Antarctic Polar Frontal Zone (PFZ) were investigated during the austral autumns April/May) of 2004 and 2005. During the 2004 survey, sub-surface (200 m) temperature profiles indicated that an intense frontal feature, formed by the convergence of the Sub-Antarctic Front (SAF) and the Antarctic Polar Front (APF) bisected the survey area into two distinct zones, the Sub- Antarctic Zone (SAZ) and the Antarctic Zone (AAZ). Total integrated chlorophyll-a (chl-a) biomass was typical for the region (< 25 mg chl-a m⁻²), and was dominated by picophytoplankton. Total euphausiid abundance and biomass ranged from 0.1 to 3.1 ind m⁻³ and 0.1 to 8.1 mg dwt m⁻³, respectively, and did not differ significantly between the stations occupied in the SAZ and AAZ (p > 0.05). The multivariate analysis identified two interacting mechanisms controlling the distribution patterns, abundance and biomass of the various euphausiid species, namely (1) diel changes in abundance and biomass, (2) and restricted distribution patterns associated with the different water masses. Ingestion rates were determined for five euphausiid species. E. triacantha was found to have the highest daily ingestion rate ranging from 1 226.1 to 6 029.1 ng pigm ind⁻¹d⁻¹, while the lowest daily ingestion rates were observed in the juvenile Thysanoessa species (6.4 to 943.0 ng pigm ind⁻¹ d⁻¹). The total grazing impact of the selected euphausiids ranged from < 0.1 to 20.1 μg pigm m⁻²d⁻¹, corresponding to < 0.15 % of the areal chl-a biomass. The daily ration estimates of autotrophic carbon for the euphausiids suggested that phytoplankton represented a minor component in their diets, with only the sub-adult E. vallentini consuming sufficient phytoplankton to meet their daily carbon requirements. A cyclonic cold-core eddy spawned from the region of the APF located in the southwest Indian sector of the PFZ was the dominant feature during the 2005 survey. The total areal chl-a biomass throughout the region was low, ranging between 5.6 and 11.4 mg chl-a m⁻², and was significantly higher within the core of the eddy compared to the surrounding waters (p < 0.05). RMT-8 and WP-2 total euphausiid abundance and biomass estimates were high, and ranged from 0.004 to 0.36 ind m⁻³ and 0.065 to 1.21 mg dwt m⁻³, and from 0.01 to 18.2 ind m⁻³ and 0.01 to 15.7 mg dwt m⁻³, respectively. A distinct spatial pattern in the euphausiid community was evident with the Antarctic species, Euphausia frigida, E. triacantha and E. superba predominating within the core of the eddy, while the PFZ waters were characterized by the sub-Antarctic species, E. longirostris, Stylocheiron maximum, Nematoscelis megalops and Thysanoessa gregaria. The eddy edge acted as a transition zone where species from both regions co-occurred. Within the survey area the combined ingestion rate of the six numerically dominant euphausiid species ranged between 0.02 and 5.31 μg pigm m⁻²d¹, which corresponded to a loss of between < 0.001 and 0.11 % of the available chl-a biomass. E. triacantha and juvenile T. macura were identified as the dominant grazers. There was no apparent spatial pattern in the grazing activity of the euphausiids within the region of investigation. The average daily rations of the euphausiids examined were < 2 % of their body carbon. The low daily ration of the euphausiids could be ascribed to the predominance of small picophytoplankton in the region of investigation, which are too small to be grazed efficiently by larger zooplankton. The marked spatial patterns in species composition and the elevated abundance and biomass of euphausiids, suggest that the mesoscale eddies contribute to the spatial and temporal heterogeneity of the planktonic community of the PFZ and may represent important foraging regions for many of the apex predators within the region.
- Full Text:
- Date Issued: 2005
The direction of trade and its implications for labour in South Africa
- Authors: Cameron, Iona R
- Date: 2005
- Subjects: Labor demand -- South Africa , Labor market -- South Africa , Industrial relations -- South Africa , Foreign trade and employment -- South Africa , International economic relations , Free trade -- South Africa , South Africa -- Commercial policy
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:948 , http://hdl.handle.net/10962/d1002682 , Labor demand -- South Africa , Labor market -- South Africa , Industrial relations -- South Africa , Foreign trade and employment -- South Africa , International economic relations , Free trade -- South Africa , South Africa -- Commercial policy
- Description: This aim of this thesis is to analyse the demand for labour from trade with a selection of South Africa’s trading partners. It is expected that labour demand will be greater in trade with developed blocs. Trade between developing blocs, however, is thought to be more skilled labour intensive and such trade should have greater linkages. This ought to feed through into greater labour demand so that South-South trade may be more ‘labour creating’ than expected. As it is more skill intensive, it may also be more dynamic, which has implications for future growth and development. Factor content methodology is used to assess labour demand. Calculations consider linkages to other sectors (which will increase labour demand) and the use of scarce resources (which has an opportunity cost to labour). The findings support the claim that trade with developing blocs is more professional labour intensive. Evidence that it may be more dynamic and have greater linkages to labour is borne out in exports to SADC. Greater labour demand through linkages, however, is not evident in net trade to SADC. Neither are they of significance in trade with any of the other developing blocs so labour effects due to linkages appear to be negligible. The advantages of South-South trade may rather lie in the dynamic benefits that trade in higher technology goods provides. When scarce resources such as capital and professional labour are taken into account, it is found that labour demand is negative in net trade to all blocs. However, even without the problem of scarce resources, most blocs have a negative demand for labour in net trade. The indication is that with the present trade patterns, South Africa cannot expect trade to increase labour demand. Policy which could improve this situation would be to increase labour force skills, improve the flexibility of the labour market and develop sectors which are both more advanced as well as labour intensive. Despite the negative impact of trade on labour in general, it is found that trade does differ by direction and that for each labour type there are certain blocs where labour demand is positive. This is also the case in net trade for particular sectors. Such information could be used as part of a targeted trade policy to assist in the marketing of particular sectors in trade and also for increasing labour demand for certain labour groups.
- Full Text:
- Date Issued: 2005
- Authors: Cameron, Iona R
- Date: 2005
- Subjects: Labor demand -- South Africa , Labor market -- South Africa , Industrial relations -- South Africa , Foreign trade and employment -- South Africa , International economic relations , Free trade -- South Africa , South Africa -- Commercial policy
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:948 , http://hdl.handle.net/10962/d1002682 , Labor demand -- South Africa , Labor market -- South Africa , Industrial relations -- South Africa , Foreign trade and employment -- South Africa , International economic relations , Free trade -- South Africa , South Africa -- Commercial policy
- Description: This aim of this thesis is to analyse the demand for labour from trade with a selection of South Africa’s trading partners. It is expected that labour demand will be greater in trade with developed blocs. Trade between developing blocs, however, is thought to be more skilled labour intensive and such trade should have greater linkages. This ought to feed through into greater labour demand so that South-South trade may be more ‘labour creating’ than expected. As it is more skill intensive, it may also be more dynamic, which has implications for future growth and development. Factor content methodology is used to assess labour demand. Calculations consider linkages to other sectors (which will increase labour demand) and the use of scarce resources (which has an opportunity cost to labour). The findings support the claim that trade with developing blocs is more professional labour intensive. Evidence that it may be more dynamic and have greater linkages to labour is borne out in exports to SADC. Greater labour demand through linkages, however, is not evident in net trade to SADC. Neither are they of significance in trade with any of the other developing blocs so labour effects due to linkages appear to be negligible. The advantages of South-South trade may rather lie in the dynamic benefits that trade in higher technology goods provides. When scarce resources such as capital and professional labour are taken into account, it is found that labour demand is negative in net trade to all blocs. However, even without the problem of scarce resources, most blocs have a negative demand for labour in net trade. The indication is that with the present trade patterns, South Africa cannot expect trade to increase labour demand. Policy which could improve this situation would be to increase labour force skills, improve the flexibility of the labour market and develop sectors which are both more advanced as well as labour intensive. Despite the negative impact of trade on labour in general, it is found that trade does differ by direction and that for each labour type there are certain blocs where labour demand is positive. This is also the case in net trade for particular sectors. Such information could be used as part of a targeted trade policy to assist in the marketing of particular sectors in trade and also for increasing labour demand for certain labour groups.
- Full Text:
- Date Issued: 2005
Operationally defining sexual orientation : towards the development of a fundamental measure of adolescent sexual responsiveness variations
- Authors: Heath, Lance Julian
- Date: 2005
- Subjects: Sexual orientation Homosexuality Psychometrics Teenagers--Sexual behavior
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1681 , http://hdl.handle.net/10962/d1003564
- Description: Much published work has pointed to the need for the development of a sound operational definition of sexual orientation in order to enable the research in this area to progress. To contribute to this process the current research set out to develop two measures of sexual orientation and examine their psychometric properties. In order to develop relevant tools historical, conceptual and operational definitions of sexual orientation were critically examined and standard questionnaire development techniques applied. The first scale consisted of 32 items and was administered to a total of 835 adolescents, comprising three sub-groups (189 Grade 11 Scholars, 547 First Year and 99 Third Year Psychology Students). A Cronbach alpha coefficient of 0.85 was calculated indicating that this instrument had very good internal consistency reliability. Similar factors emerged in each of the sample sub-groups when factor analyses were performed suggesting that this instrument has good external and construct validities. These factors each had respectable Cronbach alpha coefficients indicating their own internal consistency. The four factors which consistently emerged were Same Sex Responsiveness, Opposite Sex Responsiveness, Previous Month’s Same Sex Responsiveness and Previous Month’s Opposite Sex Responsiveness. The second scale consisted of 16 items and was administered to 646 adolescents, comprising the latter two sub-groups referred to above. A Cronbach alpha coefficient of 0.82 was calculated indicating that this instrument also had very good internal consistency reliability. Once again similar factors with generally good internal consistency emerged in factor analysis suggesting that this too was a valid instrument. The factors that emerged from the second scale were Same Sex Responsiveness, Unattractive Opposite Sex Responsiveness, Attractive Opposite Sex Responsiveness and Attraction. Future developments, adjustments and applications of the instruments as well as implications for the arena of sexual orientation research are discussed. In the light of the dearth of information with regard to the sexual orientations of South African adolescents the current study also briefly explored and presented the sample’s responses in terms of the dimensions of each questionnaire as well as how each emerging factor related to the demographics (education level, gender, sexual orientation self-label and age) of the sample.
- Full Text:
- Date Issued: 2005
- Authors: Heath, Lance Julian
- Date: 2005
- Subjects: Sexual orientation Homosexuality Psychometrics Teenagers--Sexual behavior
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1681 , http://hdl.handle.net/10962/d1003564
- Description: Much published work has pointed to the need for the development of a sound operational definition of sexual orientation in order to enable the research in this area to progress. To contribute to this process the current research set out to develop two measures of sexual orientation and examine their psychometric properties. In order to develop relevant tools historical, conceptual and operational definitions of sexual orientation were critically examined and standard questionnaire development techniques applied. The first scale consisted of 32 items and was administered to a total of 835 adolescents, comprising three sub-groups (189 Grade 11 Scholars, 547 First Year and 99 Third Year Psychology Students). A Cronbach alpha coefficient of 0.85 was calculated indicating that this instrument had very good internal consistency reliability. Similar factors emerged in each of the sample sub-groups when factor analyses were performed suggesting that this instrument has good external and construct validities. These factors each had respectable Cronbach alpha coefficients indicating their own internal consistency. The four factors which consistently emerged were Same Sex Responsiveness, Opposite Sex Responsiveness, Previous Month’s Same Sex Responsiveness and Previous Month’s Opposite Sex Responsiveness. The second scale consisted of 16 items and was administered to 646 adolescents, comprising the latter two sub-groups referred to above. A Cronbach alpha coefficient of 0.82 was calculated indicating that this instrument also had very good internal consistency reliability. Once again similar factors with generally good internal consistency emerged in factor analysis suggesting that this too was a valid instrument. The factors that emerged from the second scale were Same Sex Responsiveness, Unattractive Opposite Sex Responsiveness, Attractive Opposite Sex Responsiveness and Attraction. Future developments, adjustments and applications of the instruments as well as implications for the arena of sexual orientation research are discussed. In the light of the dearth of information with regard to the sexual orientations of South African adolescents the current study also briefly explored and presented the sample’s responses in terms of the dimensions of each questionnaire as well as how each emerging factor related to the demographics (education level, gender, sexual orientation self-label and age) of the sample.
- Full Text:
- Date Issued: 2005
Interest rate behaviour in a more transparent South African monetary policy environment
- Authors: Ballim, Goolam Hoosen
- Date: 2005
- Subjects: South African Reserve Bank , Monetary policy -- South Africa , Banks and banking -- South Africa , Interest rates -- South Africa , South Africa -- Economic policy , South Africa -- Economic conditions
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1034 , http://hdl.handle.net/10962/d1004462 , South African Reserve Bank , Monetary policy -- South Africa , Banks and banking -- South Africa , Interest rates -- South Africa , South Africa -- Economic policy , South Africa -- Economic conditions
- Description: South Africa introduced inflation targeting as a monetary policy framework in 2000. This marked a sizable shift in monetary policy management from the previous "eclectic" approach and the explicit focus on M3 money supply before that. The study appraises the effectiveness of monetary policy under this new dispensation. However, the analysis does not centre on inflation outcomes, which can be a measure of effectiveness because they are the overriding objective of the South African Reserve Bank in effect, it is possible to have a target-friendly inflation rate for a length of time despite monetary policy that is ambiguous and encourages unpredictability in market interest rates. However, persistent policy opaqueness can, over time, damage a favourable inflation scenario. For instance, if the public is unsure about the Reserve Bank's desired inflation target, price setting in the wage and goods markets may eventually produce an inflation outcome that is higher than the Bank may have intended. Rather, this study adjudicates the effectiveness of monetary policy within the context of policy transparency, which is an intrinsic part of the inflation targeting framework. The study looks at the extent to which monetary policy transparency has enhanced both the anticipatory nature of the market's response to policy actions and the force that policy has on all interest rates in the financial system, particularly long-term rates. These concepts are important because through the transmission mechanism of monetary policy, the more deft market participants are at anticipating future Reserve Bank policy the greater the Bank's ability to steady the economy before the actual policy event. With the aid of regression models to estimate the response of market rates to policy changes, the results show that there is significant movement in market rates in anticipation of policy action, rather than on the day of the event or the day after. Indeed, the estimates for market rates movement on the day of and even the day after the policy action are generally minute. For instance, the R157 long-term government bond yield changes by a significant 41 basis points in response to a one percentage point change in the Reserve Bank's benchmark repo rate in the period between the last policy action and the day preceding the current action. In contrast, the R157 bond yield changes by an insignificant 2 basis points on the day of the current repo rate change and about 1 basis point the day after the current change. The results point to a robust relationship between policy transparency and the market's ability to foresee rate action. If this were not the case, it is likely that there would be persistent market surprise and, hence, noticeable movement in interest rates on the day of the rate action and perhaps even the day after. Another important observation is that monetary policy impacts significantly on both short- and long-term market rates. Again, certifying the robustness of monetary policy under the inflation targeting regime
- Full Text:
- Date Issued: 2005
- Authors: Ballim, Goolam Hoosen
- Date: 2005
- Subjects: South African Reserve Bank , Monetary policy -- South Africa , Banks and banking -- South Africa , Interest rates -- South Africa , South Africa -- Economic policy , South Africa -- Economic conditions
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1034 , http://hdl.handle.net/10962/d1004462 , South African Reserve Bank , Monetary policy -- South Africa , Banks and banking -- South Africa , Interest rates -- South Africa , South Africa -- Economic policy , South Africa -- Economic conditions
- Description: South Africa introduced inflation targeting as a monetary policy framework in 2000. This marked a sizable shift in monetary policy management from the previous "eclectic" approach and the explicit focus on M3 money supply before that. The study appraises the effectiveness of monetary policy under this new dispensation. However, the analysis does not centre on inflation outcomes, which can be a measure of effectiveness because they are the overriding objective of the South African Reserve Bank in effect, it is possible to have a target-friendly inflation rate for a length of time despite monetary policy that is ambiguous and encourages unpredictability in market interest rates. However, persistent policy opaqueness can, over time, damage a favourable inflation scenario. For instance, if the public is unsure about the Reserve Bank's desired inflation target, price setting in the wage and goods markets may eventually produce an inflation outcome that is higher than the Bank may have intended. Rather, this study adjudicates the effectiveness of monetary policy within the context of policy transparency, which is an intrinsic part of the inflation targeting framework. The study looks at the extent to which monetary policy transparency has enhanced both the anticipatory nature of the market's response to policy actions and the force that policy has on all interest rates in the financial system, particularly long-term rates. These concepts are important because through the transmission mechanism of monetary policy, the more deft market participants are at anticipating future Reserve Bank policy the greater the Bank's ability to steady the economy before the actual policy event. With the aid of regression models to estimate the response of market rates to policy changes, the results show that there is significant movement in market rates in anticipation of policy action, rather than on the day of the event or the day after. Indeed, the estimates for market rates movement on the day of and even the day after the policy action are generally minute. For instance, the R157 long-term government bond yield changes by a significant 41 basis points in response to a one percentage point change in the Reserve Bank's benchmark repo rate in the period between the last policy action and the day preceding the current action. In contrast, the R157 bond yield changes by an insignificant 2 basis points on the day of the current repo rate change and about 1 basis point the day after the current change. The results point to a robust relationship between policy transparency and the market's ability to foresee rate action. If this were not the case, it is likely that there would be persistent market surprise and, hence, noticeable movement in interest rates on the day of the rate action and perhaps even the day after. Another important observation is that monetary policy impacts significantly on both short- and long-term market rates. Again, certifying the robustness of monetary policy under the inflation targeting regime
- Full Text:
- Date Issued: 2005
Re-inventing educational leadership for school and community transformation: learning from the Educational Leadership Management and Development programme of the University of Fort Hare
- Authors: Moyo, George
- Date: 2005
- Subjects: University of Fort Hare. Educational Leadership Management and Development Educational leadership -- South Africa Educational change -- South Africa School management and organization -- South Africa School improvement programs -- South Africa School principals -- South Africa Community and school -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1719 , http://hdl.handle.net/10962/d1003602
- Description: This study explores educational leadership development and social change strategies pioneered by one programme, the Educational Leadership Management and Development (ELMD) programme of the University of Fort Hare. The programme seeks to model a way of doing social and educational transformation through educational leadership development. Conceptually, the model was meant to draw together a number of education stakeholders operating at various levels of the schooling system to undergo the same programme of leadership development. The programme participants, who included district education officials, schools principals, members of school management teams, educators and members of School Governing Bodies, were to enrol as teams. They would work on learning tasks that were both academic and practical in nature, with an emphasis on experiential learning that leads to the creation of district and community networks of partners, development teams or forums and communities of practice, as well as the production and implementation of district and school development plans. Informed by this conceptual position, the study was structured by two underlying questions. First, whether the ELMD was re-inventing educational leadership beyond the traditional focus on principalship towards one that is inclusive of other education stakeholders. Second, how leadership development as a vehicle for social and educational change can be carried out. The research process was guided by a multi-paradigm perspective which drew heavily on the interpretive and critical science orientations. This led to the crafting of research methods that looked for data that would assist in an understanding of what was happening in the programme, as well as what power dynamics were at play and with what consequences for innovation. The evidence emanating from the study suggests a number of possibilities for consideration by future leadership development programme designers. First, the ELMD programme delivery design shows what can be done to draw participants from various levels of the schooling system, district, school and community and teach them educational leadership together in a mode that mobilizes them for change. Second, how social distance separating different levels of the education hierarchy and status consciousness may disappear gradually as people are brought together to work on tasks of mutual concern. Third, after a year of engagement with ELMD ideas and approach, the participants in the programme appeared to have started a journey of selftransformation towards becoming qualitatively different people who saw themselves as teams capable of tackling education and social problems in their schools and communities. These participants had begun to forge working networks, but the extent to which these could be characterized as knowledge ecosystems and communities of practice remains a question to explore. Fourth, that the current higher education accreditation policies and practices do not accommodate innovative learning approaches of the kind that the ELMD is developing. In this regard, the ELMD experienced difficulties in coming up with an assessment policy and practices which meet the academic as well as the practical developmental concerns of the programme. Fifth, programme instrumentalities and mandates that are put in place do not, in themselves, bring about change. The actual change comes about through the actions of human leadership capable of navigating between structural enablers and constraints.
- Full Text:
- Date Issued: 2005
- Authors: Moyo, George
- Date: 2005
- Subjects: University of Fort Hare. Educational Leadership Management and Development Educational leadership -- South Africa Educational change -- South Africa School management and organization -- South Africa School improvement programs -- South Africa School principals -- South Africa Community and school -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1719 , http://hdl.handle.net/10962/d1003602
- Description: This study explores educational leadership development and social change strategies pioneered by one programme, the Educational Leadership Management and Development (ELMD) programme of the University of Fort Hare. The programme seeks to model a way of doing social and educational transformation through educational leadership development. Conceptually, the model was meant to draw together a number of education stakeholders operating at various levels of the schooling system to undergo the same programme of leadership development. The programme participants, who included district education officials, schools principals, members of school management teams, educators and members of School Governing Bodies, were to enrol as teams. They would work on learning tasks that were both academic and practical in nature, with an emphasis on experiential learning that leads to the creation of district and community networks of partners, development teams or forums and communities of practice, as well as the production and implementation of district and school development plans. Informed by this conceptual position, the study was structured by two underlying questions. First, whether the ELMD was re-inventing educational leadership beyond the traditional focus on principalship towards one that is inclusive of other education stakeholders. Second, how leadership development as a vehicle for social and educational change can be carried out. The research process was guided by a multi-paradigm perspective which drew heavily on the interpretive and critical science orientations. This led to the crafting of research methods that looked for data that would assist in an understanding of what was happening in the programme, as well as what power dynamics were at play and with what consequences for innovation. The evidence emanating from the study suggests a number of possibilities for consideration by future leadership development programme designers. First, the ELMD programme delivery design shows what can be done to draw participants from various levels of the schooling system, district, school and community and teach them educational leadership together in a mode that mobilizes them for change. Second, how social distance separating different levels of the education hierarchy and status consciousness may disappear gradually as people are brought together to work on tasks of mutual concern. Third, after a year of engagement with ELMD ideas and approach, the participants in the programme appeared to have started a journey of selftransformation towards becoming qualitatively different people who saw themselves as teams capable of tackling education and social problems in their schools and communities. These participants had begun to forge working networks, but the extent to which these could be characterized as knowledge ecosystems and communities of practice remains a question to explore. Fourth, that the current higher education accreditation policies and practices do not accommodate innovative learning approaches of the kind that the ELMD is developing. In this regard, the ELMD experienced difficulties in coming up with an assessment policy and practices which meet the academic as well as the practical developmental concerns of the programme. Fifth, programme instrumentalities and mandates that are put in place do not, in themselves, bring about change. The actual change comes about through the actions of human leadership capable of navigating between structural enablers and constraints.
- Full Text:
- Date Issued: 2005
The early proterozoic Makganyene glacial event in South Africa : its implication in sequence stratigraphy interpretations, paleoenvironmental conditions and iron and manganese ore deposition
- Authors: Polteau, Stéphane
- Date: 2005
- Subjects: Geology, Stratigraphic -- South Africa -- Northern Cape Geochemistry -- South Africa -- Northern Cape Paleogeography -- South Africa -- Northern Cape Petrology -- South Africa -- Northern Cape Ore deposits -- South Africa -- Northern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5039 , http://hdl.handle.net/10962/d1007612
- Description: The Makganyene Formation forms the base of the Postmasburg Group in the Transvaal Supergroup in the Griqualand West Basin. It consists of diamictites, sandstones, banded iron-formations (BIFs), shales, siltstones and carbonates. It is generally accepted that the Makganyene Formation rests on an erosive regional unconformity throughout the Northern Cape Province. However this study demonstrates that this stratigraphic relationship is not universal, and conformable contacts have been observed. One of the principal aims of this study is to identify the nature of the Makganyene basal contact throughout the Griqualand West Basin. Intensive fieldwork was carried out from Prieska in the south, to Danielskuil in the north. In the Sishen and Hotazel areas, only borehole material was available to assess the stratigraphy. The Griquatown Fault Zone delimits the boundary between the deep basin and platform facies. The Koegas Subgroup is only present south of the Griquatown Fault Zone, where it pinches out. However, the transition Griquatown BIFs-Koegas Subgroup occurs in lacustrine deposits on the Ghaap platform (Beukes, 1983). The Griquatown Fault Zone represents the edge of the basin, which corresponds to a hinge rather than a fault zone. The Makganyene Formation rests with a conformable contact on the Koegas Subgroup south of the Griquatown Hinge Zone, and north of it the Makganyene Formation lies unconformably on the Asbestos Hills Subgroup. The Makganyene Formation displays lateral facies changes that reflect the paleogeography of the Griqualand West Basin, and the development of ice sheets/shelves. The Ghaap platform is characterised by coarse immature sand interbedded with the diamictites. The clasts in this area contain local Asbestos Hills material and no dropstones are present. Such settings are typical of sediments that are being deposited below a grounded ice mass. At the Griquatown Hinge Zone, the sandstone lenses are smaller, and the clasts consist of chert, of which a great number are striated and faceted. In the Matsap area, the presence of dropstones is strong evidence for the presence of a floating ice shelf that released its material by basal melting. Further south, the Makganyene Formation contains stromatolitic bioherms that only form if clastic contamination is minimal and therefore the ice that transported the detritus to the basin did not extend far into open sea conditions. The base of the Hotazel Formation also contains diamictite levels. Dropstones have been identified, implying a glacial origin. The Hotazel diamictites are interbedded with hyaloclastites and BIFs. The Makganyene glacial event, therefore, was not restricted to the Makganyene Formation, but also included the Ongeluk Formation, through to the base of the Hotazel Formation. Petrographic studies of the Makganyene Formation and the base of the Hotazel Formation reveal mineral assemblages that are diagnostic of early to late diagenetic crystallisation and of low-grade metamorphism not exceeding the very low green-schist facies. The facies identified display the same sense of basin deepening, from shallow high-energy Hotazel area on the Ghaap platform, to the deep basin in the Matsap area. Whole-rock geochemical analyses reveal that the elemental composition of the Makganyene Formation is very similar to that of the Asbestos Hills BIFs, which were the most important source of clastic detritus for the Makganyene Formation. However, minor amounts of carbonates of the Campbellrand Subgroup, as well as a felsic crustal input from the Archean granitoid basement, made contributions. On the Ghaap platform, the Makganyene diamictite is enriched in iron, calcium, and magnesium, while in the deeper parts of the basin the diamictites are enriched in detrital elements, such as titanium and aluminium, which occur in the fine clay component. The Hotazel diamictite displays a distinct mafic volcanic input, related to the extrusion of the Ongeluk basaltic andesites, which was incorporated in the glacial sediments. Sequence stratigraphy is based on the recognition of contacts separating the different systems tracts that compose a depositional sequence. However, because the basal contact of the Makganyene Formation has not been properly identified in previous work, no correct model has been proposed so far. Therefore correlations between the Griqualand West and the Transvaal basins, based on lithostratigraphic similarities and extrapolations of unconformities, have to be reviewed, especially since the publication of new radiometric ages contradict all previously proposed correlations. It is proposed here that the Transvaal Supergroup in the Griqualand West Basin represents a continuous depositional event that lasted about 200 Ma. The Makganyene glacial event occurred during changing conditions in the chemistries of the atmosphere and ocean, and in the continental configuration. A Snowball Earth event has been proposed as the causative process of such paleoenvironmental changes. However, evidence presented here of less dramatic glacial conditions, with areas of ice-free waters, implies an alternative to the Snowball Earth event. The paleoenvironmental changes are thought to represent a transition from an anaerobic to aerobic atmosphere, that was responsible for the global cooling of the surface of the Earth, Such a glacial event may have aided in the large-scale precipitation of iron and manganese in areas of intense upwellings.
- Full Text:
- Date Issued: 2005
- Authors: Polteau, Stéphane
- Date: 2005
- Subjects: Geology, Stratigraphic -- South Africa -- Northern Cape Geochemistry -- South Africa -- Northern Cape Paleogeography -- South Africa -- Northern Cape Petrology -- South Africa -- Northern Cape Ore deposits -- South Africa -- Northern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5039 , http://hdl.handle.net/10962/d1007612
- Description: The Makganyene Formation forms the base of the Postmasburg Group in the Transvaal Supergroup in the Griqualand West Basin. It consists of diamictites, sandstones, banded iron-formations (BIFs), shales, siltstones and carbonates. It is generally accepted that the Makganyene Formation rests on an erosive regional unconformity throughout the Northern Cape Province. However this study demonstrates that this stratigraphic relationship is not universal, and conformable contacts have been observed. One of the principal aims of this study is to identify the nature of the Makganyene basal contact throughout the Griqualand West Basin. Intensive fieldwork was carried out from Prieska in the south, to Danielskuil in the north. In the Sishen and Hotazel areas, only borehole material was available to assess the stratigraphy. The Griquatown Fault Zone delimits the boundary between the deep basin and platform facies. The Koegas Subgroup is only present south of the Griquatown Fault Zone, where it pinches out. However, the transition Griquatown BIFs-Koegas Subgroup occurs in lacustrine deposits on the Ghaap platform (Beukes, 1983). The Griquatown Fault Zone represents the edge of the basin, which corresponds to a hinge rather than a fault zone. The Makganyene Formation rests with a conformable contact on the Koegas Subgroup south of the Griquatown Hinge Zone, and north of it the Makganyene Formation lies unconformably on the Asbestos Hills Subgroup. The Makganyene Formation displays lateral facies changes that reflect the paleogeography of the Griqualand West Basin, and the development of ice sheets/shelves. The Ghaap platform is characterised by coarse immature sand interbedded with the diamictites. The clasts in this area contain local Asbestos Hills material and no dropstones are present. Such settings are typical of sediments that are being deposited below a grounded ice mass. At the Griquatown Hinge Zone, the sandstone lenses are smaller, and the clasts consist of chert, of which a great number are striated and faceted. In the Matsap area, the presence of dropstones is strong evidence for the presence of a floating ice shelf that released its material by basal melting. Further south, the Makganyene Formation contains stromatolitic bioherms that only form if clastic contamination is minimal and therefore the ice that transported the detritus to the basin did not extend far into open sea conditions. The base of the Hotazel Formation also contains diamictite levels. Dropstones have been identified, implying a glacial origin. The Hotazel diamictites are interbedded with hyaloclastites and BIFs. The Makganyene glacial event, therefore, was not restricted to the Makganyene Formation, but also included the Ongeluk Formation, through to the base of the Hotazel Formation. Petrographic studies of the Makganyene Formation and the base of the Hotazel Formation reveal mineral assemblages that are diagnostic of early to late diagenetic crystallisation and of low-grade metamorphism not exceeding the very low green-schist facies. The facies identified display the same sense of basin deepening, from shallow high-energy Hotazel area on the Ghaap platform, to the deep basin in the Matsap area. Whole-rock geochemical analyses reveal that the elemental composition of the Makganyene Formation is very similar to that of the Asbestos Hills BIFs, which were the most important source of clastic detritus for the Makganyene Formation. However, minor amounts of carbonates of the Campbellrand Subgroup, as well as a felsic crustal input from the Archean granitoid basement, made contributions. On the Ghaap platform, the Makganyene diamictite is enriched in iron, calcium, and magnesium, while in the deeper parts of the basin the diamictites are enriched in detrital elements, such as titanium and aluminium, which occur in the fine clay component. The Hotazel diamictite displays a distinct mafic volcanic input, related to the extrusion of the Ongeluk basaltic andesites, which was incorporated in the glacial sediments. Sequence stratigraphy is based on the recognition of contacts separating the different systems tracts that compose a depositional sequence. However, because the basal contact of the Makganyene Formation has not been properly identified in previous work, no correct model has been proposed so far. Therefore correlations between the Griqualand West and the Transvaal basins, based on lithostratigraphic similarities and extrapolations of unconformities, have to be reviewed, especially since the publication of new radiometric ages contradict all previously proposed correlations. It is proposed here that the Transvaal Supergroup in the Griqualand West Basin represents a continuous depositional event that lasted about 200 Ma. The Makganyene glacial event occurred during changing conditions in the chemistries of the atmosphere and ocean, and in the continental configuration. A Snowball Earth event has been proposed as the causative process of such paleoenvironmental changes. However, evidence presented here of less dramatic glacial conditions, with areas of ice-free waters, implies an alternative to the Snowball Earth event. The paleoenvironmental changes are thought to represent a transition from an anaerobic to aerobic atmosphere, that was responsible for the global cooling of the surface of the Earth, Such a glacial event may have aided in the large-scale precipitation of iron and manganese in areas of intense upwellings.
- Full Text:
- Date Issued: 2005
The historiographical development of the concept "mfecane" and the writing of early Southern African history, from the 1820s to 1920s
- Authors: Richner, Jürg Emile
- Date: 2005
- Subjects: South Africa -- History , South Africa -- Historiography , Bantu-speaking peoples -- Migrations , Africa, Southern -- History -- Mfecane period, 1816-ca. 1840
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2554 , http://hdl.handle.net/10962/d1002406 , South Africa -- History , South Africa -- Historiography , Bantu-speaking peoples -- Migrations , Africa, Southern -- History -- Mfecane period, 1816-ca. 1840
- Description: The mfecane was for most of the twentieth century regarded as a historical certainty for the South African public and the Apartheid government, as well as for historians here and world-wide. The mfecane had achieved the permanence of a paradigm and a dominant discourse, as it was accepted equally by settler, liberal, Afrikaner, Africanist and Neo-Marxist historians. This certainty was shaken when Cobbing’s mfecane critique appeared in 1988, with which I concur. This study examines how mfecane history was written from the first published articles in mid-1823 until Walker coined the concept mfecane in 1928. This thesis undertakes a journey through a host of published works, books, pamphlets and articles in journals, magazines and newspapers, from which a number of conclusions emerge. The mfecane narrative was developed over a period of a hundred years in the English language by almost exclusively white, English-speaking male amateur historians and ethnographers. Their occupations, age, ideology and level of education differed markedly, but they shared one European ideological value, the discourse of the European “Image of Africa”, which regarded Africans as the negative Other of their own positively perceived society. This was a culturallyshared view of Africans, which formed the baggage in the mind of all writers examined, and accounts for the mfecane narrative’s negative attitude towards Africans. Furthermore, mfecane history was influenced by racism and the use of literary devices such as the gothic novel and the romance. Authors writing in the 1823 to 1846 period on events which had taken place in the “blank space” beyond the Cape Colony, which most of them had never visited, laid the basis for the mfecane narrative. It constituted a set of geographical or ethnically focused, separate accounts. These separate accounts focused on the themes of Shaka’s creation of the Zulu state, including his expulsion of several chiefdoms; his depopulation of Natal and the flight of the Fingo to the Transkei; the path of destruction of the Hlubi and Ngwane during their flight from Natal via the greater Caledon Valley area to the Transkei; the incorporation of the Kololo and other Sotho chiefdoms into the Mantatees - due to pressure from the invaders from Natal - who subsequently laid waste the Free State and Transvaal as far as Dithakong, where they were defeated; the further depopulation of the Transvaal by the Ndebele during their escape from Shaka; the flight of Moshoeshoe and his people to Thaba Bosiu where he built up the Sotho state, with Moshoeshoe being the only positive figure in this history. This multi-narrative was thereafter repeated without any critical thought by all authors examined until in 1885 Theal created a Zulu-centric, geographically integrated mfecane narrative whereby he integrated the previously separate accounts into one coherent whole - a whole which was so much more than the sum of its parts, but so far without a defining name. That was provided by Walker in 1928 when he coined the Xhosa neologism, mfecane. The Theal, Cory and Walker racist mfecane was thus bequeathed as the mfecane to the rest of the twentieth century.
- Full Text:
- Date Issued: 2005
- Authors: Richner, Jürg Emile
- Date: 2005
- Subjects: South Africa -- History , South Africa -- Historiography , Bantu-speaking peoples -- Migrations , Africa, Southern -- History -- Mfecane period, 1816-ca. 1840
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2554 , http://hdl.handle.net/10962/d1002406 , South Africa -- History , South Africa -- Historiography , Bantu-speaking peoples -- Migrations , Africa, Southern -- History -- Mfecane period, 1816-ca. 1840
- Description: The mfecane was for most of the twentieth century regarded as a historical certainty for the South African public and the Apartheid government, as well as for historians here and world-wide. The mfecane had achieved the permanence of a paradigm and a dominant discourse, as it was accepted equally by settler, liberal, Afrikaner, Africanist and Neo-Marxist historians. This certainty was shaken when Cobbing’s mfecane critique appeared in 1988, with which I concur. This study examines how mfecane history was written from the first published articles in mid-1823 until Walker coined the concept mfecane in 1928. This thesis undertakes a journey through a host of published works, books, pamphlets and articles in journals, magazines and newspapers, from which a number of conclusions emerge. The mfecane narrative was developed over a period of a hundred years in the English language by almost exclusively white, English-speaking male amateur historians and ethnographers. Their occupations, age, ideology and level of education differed markedly, but they shared one European ideological value, the discourse of the European “Image of Africa”, which regarded Africans as the negative Other of their own positively perceived society. This was a culturallyshared view of Africans, which formed the baggage in the mind of all writers examined, and accounts for the mfecane narrative’s negative attitude towards Africans. Furthermore, mfecane history was influenced by racism and the use of literary devices such as the gothic novel and the romance. Authors writing in the 1823 to 1846 period on events which had taken place in the “blank space” beyond the Cape Colony, which most of them had never visited, laid the basis for the mfecane narrative. It constituted a set of geographical or ethnically focused, separate accounts. These separate accounts focused on the themes of Shaka’s creation of the Zulu state, including his expulsion of several chiefdoms; his depopulation of Natal and the flight of the Fingo to the Transkei; the path of destruction of the Hlubi and Ngwane during their flight from Natal via the greater Caledon Valley area to the Transkei; the incorporation of the Kololo and other Sotho chiefdoms into the Mantatees - due to pressure from the invaders from Natal - who subsequently laid waste the Free State and Transvaal as far as Dithakong, where they were defeated; the further depopulation of the Transvaal by the Ndebele during their escape from Shaka; the flight of Moshoeshoe and his people to Thaba Bosiu where he built up the Sotho state, with Moshoeshoe being the only positive figure in this history. This multi-narrative was thereafter repeated without any critical thought by all authors examined until in 1885 Theal created a Zulu-centric, geographically integrated mfecane narrative whereby he integrated the previously separate accounts into one coherent whole - a whole which was so much more than the sum of its parts, but so far without a defining name. That was provided by Walker in 1928 when he coined the Xhosa neologism, mfecane. The Theal, Cory and Walker racist mfecane was thus bequeathed as the mfecane to the rest of the twentieth century.
- Full Text:
- Date Issued: 2005
A comparative bioinformatic analysis of zinc binuclear cluster proteins
- Authors: Mthombeni, Jabulani S
- Date: 2005
- Subjects: Bioinformatics , Zinc proteins , GABA
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4004 , http://hdl.handle.net/10962/d1004064 , Bioinformatics , Zinc proteins , GABA
- Description: Members of the zinc binuclear cluster family are important fungal transcriptional regulators sharing a common DNA binding domain. Da181p is a pleotropic zinc binuclear cluster protein involved in the induction of the UGA genes required for the γ-aminobutyrate nitrogen catabolic pathway in Saccharomyces cerevisiae. The zinc binuclear cluster domain is indispensable for function in Da181p and little is known about other domains in this protein. The aim of the study was to explore the zinc binuclear cluster protein family using comparative bioinformatics as a complement to biochemical and structural approaches. A database of all zinc binuclear cluster proteins was composed. A total of 118 zinc binuclear proteins are reported in this work. Thirty nine previously unidentified zinc binuclear cluster proteins were found. Four homologues of Da181p were identified by homology searching. Important sequence motifs were identified in the aligned sequences of Da181p and its homologues. The coiled coil motif found in the Ga14p zinc binuclear cluster protein could not be identified in Da181p and its homologues. This suggested that Da181p did not dimerise through this structural motif as other zinc binuclear cluster proteins. Solvent accessible site that could be phosphorylated by protein kinase C or casein kinase II and the role of such sites in the possible regulation of Da181p function were discussed.
- Full Text:
- Date Issued: 2005
- Authors: Mthombeni, Jabulani S
- Date: 2005
- Subjects: Bioinformatics , Zinc proteins , GABA
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4004 , http://hdl.handle.net/10962/d1004064 , Bioinformatics , Zinc proteins , GABA
- Description: Members of the zinc binuclear cluster family are important fungal transcriptional regulators sharing a common DNA binding domain. Da181p is a pleotropic zinc binuclear cluster protein involved in the induction of the UGA genes required for the γ-aminobutyrate nitrogen catabolic pathway in Saccharomyces cerevisiae. The zinc binuclear cluster domain is indispensable for function in Da181p and little is known about other domains in this protein. The aim of the study was to explore the zinc binuclear cluster protein family using comparative bioinformatics as a complement to biochemical and structural approaches. A database of all zinc binuclear cluster proteins was composed. A total of 118 zinc binuclear proteins are reported in this work. Thirty nine previously unidentified zinc binuclear cluster proteins were found. Four homologues of Da181p were identified by homology searching. Important sequence motifs were identified in the aligned sequences of Da181p and its homologues. The coiled coil motif found in the Ga14p zinc binuclear cluster protein could not be identified in Da181p and its homologues. This suggested that Da181p did not dimerise through this structural motif as other zinc binuclear cluster proteins. Solvent accessible site that could be phosphorylated by protein kinase C or casein kinase II and the role of such sites in the possible regulation of Da181p function were discussed.
- Full Text:
- Date Issued: 2005
A framework for evaluating instructional design models resulting in a model for designing and developing computer based learning tools with GIS technologies
- Authors: Stott, Deborah Ann
- Date: 2005
- Subjects: Computer-assisted instruction Geographic information systems Education, Secondary -- Computer network resources Educational innovations
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1548 , http://hdl.handle.net/10962/d1003430
- Description: With the increasing pressures and concerns in education regarding capability, lifelong learning, higher order cognitive skills, transdisciplinary education and so on, educators are seeking fresh ways to teach and new tools to support that teaching. In South Africa, Outcomes Based Education (OBE) has identified critical outcomes (skills) across all subject areas such as problem solving, teamwork, fostering critical thinking etc. as a way of responding to these pressures and concerns. But OBE has been criticised for lacking the necessary tools to develop these critical skills and to promote cross-discipline learning. One innovative way of offering transformative teaching, instruction and learning that may foster the development of these critical skills, particularly those concerned with critical thinking, is by using geographic information systems (GIS) technologies. The scope for using these technologies in secondary education is now being realised for teaching the more generic, cross-discipline skills described whereby students are learning not only about GIS but also with GIS. This realisation provides the opportunity to create flexible, computer-based learning materials that are rooted in authentic, real-world contexts, which aim to enhance the cognitive skills of the students. If these technologies can be used in an innovative way to develop critical outcomes and skills, a model needs to be defined to aid the design and development of learning materials using these technologies for use in schools. The primary aim of this study has been to develop such a model; a model which emphasises the development of real-world learning materials that develop higher-order thinking skills in learners. Another key product of this study is the submission of a comprehensive yet flexible framework for evaluating instructional design models found in the educational literature in order to determine if these design models can be used to develop learning materials forparticular contexts.
- Full Text:
- Date Issued: 2005
- Authors: Stott, Deborah Ann
- Date: 2005
- Subjects: Computer-assisted instruction Geographic information systems Education, Secondary -- Computer network resources Educational innovations
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1548 , http://hdl.handle.net/10962/d1003430
- Description: With the increasing pressures and concerns in education regarding capability, lifelong learning, higher order cognitive skills, transdisciplinary education and so on, educators are seeking fresh ways to teach and new tools to support that teaching. In South Africa, Outcomes Based Education (OBE) has identified critical outcomes (skills) across all subject areas such as problem solving, teamwork, fostering critical thinking etc. as a way of responding to these pressures and concerns. But OBE has been criticised for lacking the necessary tools to develop these critical skills and to promote cross-discipline learning. One innovative way of offering transformative teaching, instruction and learning that may foster the development of these critical skills, particularly those concerned with critical thinking, is by using geographic information systems (GIS) technologies. The scope for using these technologies in secondary education is now being realised for teaching the more generic, cross-discipline skills described whereby students are learning not only about GIS but also with GIS. This realisation provides the opportunity to create flexible, computer-based learning materials that are rooted in authentic, real-world contexts, which aim to enhance the cognitive skills of the students. If these technologies can be used in an innovative way to develop critical outcomes and skills, a model needs to be defined to aid the design and development of learning materials using these technologies for use in schools. The primary aim of this study has been to develop such a model; a model which emphasises the development of real-world learning materials that develop higher-order thinking skills in learners. Another key product of this study is the submission of a comprehensive yet flexible framework for evaluating instructional design models found in the educational literature in order to determine if these design models can be used to develop learning materials forparticular contexts.
- Full Text:
- Date Issued: 2005
Development of experimental systems for studying the biology of Nudaurelia capensis ß virus
- Authors: Walter, Cheryl Tracy
- Date: 2005
- Subjects: Imbrasia cytherea , Insects -- Viruses , RNA viruses , DNA
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3948 , http://hdl.handle.net/10962/d1004007 , Imbrasia cytherea , Insects -- Viruses , RNA viruses , DNA
- Description: After 20 years, Nudaurelia ß virus (NßV) was re-isolated from a population of Nudaurelia capensis larvae exhibiting similar symptoms to those described in 1974 for a tetravirus infection. NßV is a member of the Tetraviridae, a family of positive sense insect RNA viruses that exclusively infect Lepidopteran insects. In addition to NbV, there was evidence that the insects were infected with another small RNA virus. SDS-PAGE and Western analysis revealed two proteins (p56 and p58), that cross-reacted with anti-NbV antibodies. Transmission Electron Microscopy (TEM) analysis showed the presence of particles exhibiting a morphology described for NbV and majority of particles of a diameter of 37 nm. In addition there was a second, minor population of particles with a diameter of 34 nm, which also exhibited the characteristic pitted surface of NßV, raising the possibility of two species of NßV in the N. capensis population. To further investigate this, cDNA corresponding to the 3` end of the replicase gene as well as the entire capsid gene of NbV was synthesized and sequenced. Alignments of the cDNA sequence showed a 99.46 % identity to the published sequence of NbV. Two amino acid substitutions were observed in the capsid coding sequence, one of which was a conservative substitution. Both of these substitutions were found in the b-sandwich domain of the capsid protein. Inspection of the capsid coding sequence showed a second methionine (Met50) at the VCAP amino terminus raising the possibility that p56 might arise from a translation product starting at this site. To investigate this, a full length and truncated capsid coding sequence starting at Met50, were expressed in a baculovirus expression system. VLPs were examined by TEM and Western analysis showed the presence of virus like particles with NßV morphology, but smaller in diameter than the wild-type with an average of 33.33 nm, similar to the smaller particles observed in the virus preparations of NßV. This result supported the hypothesis that NßV translates a smaller coat protein from the second in-frame methionine residue.
- Full Text:
- Date Issued: 2005
- Authors: Walter, Cheryl Tracy
- Date: 2005
- Subjects: Imbrasia cytherea , Insects -- Viruses , RNA viruses , DNA
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3948 , http://hdl.handle.net/10962/d1004007 , Imbrasia cytherea , Insects -- Viruses , RNA viruses , DNA
- Description: After 20 years, Nudaurelia ß virus (NßV) was re-isolated from a population of Nudaurelia capensis larvae exhibiting similar symptoms to those described in 1974 for a tetravirus infection. NßV is a member of the Tetraviridae, a family of positive sense insect RNA viruses that exclusively infect Lepidopteran insects. In addition to NbV, there was evidence that the insects were infected with another small RNA virus. SDS-PAGE and Western analysis revealed two proteins (p56 and p58), that cross-reacted with anti-NbV antibodies. Transmission Electron Microscopy (TEM) analysis showed the presence of particles exhibiting a morphology described for NbV and majority of particles of a diameter of 37 nm. In addition there was a second, minor population of particles with a diameter of 34 nm, which also exhibited the characteristic pitted surface of NßV, raising the possibility of two species of NßV in the N. capensis population. To further investigate this, cDNA corresponding to the 3` end of the replicase gene as well as the entire capsid gene of NbV was synthesized and sequenced. Alignments of the cDNA sequence showed a 99.46 % identity to the published sequence of NbV. Two amino acid substitutions were observed in the capsid coding sequence, one of which was a conservative substitution. Both of these substitutions were found in the b-sandwich domain of the capsid protein. Inspection of the capsid coding sequence showed a second methionine (Met50) at the VCAP amino terminus raising the possibility that p56 might arise from a translation product starting at this site. To investigate this, a full length and truncated capsid coding sequence starting at Met50, were expressed in a baculovirus expression system. VLPs were examined by TEM and Western analysis showed the presence of virus like particles with NßV morphology, but smaller in diameter than the wild-type with an average of 33.33 nm, similar to the smaller particles observed in the virus preparations of NßV. This result supported the hypothesis that NßV translates a smaller coat protein from the second in-frame methionine residue.
- Full Text:
- Date Issued: 2005
The life history patterns of the polychaete, Terebrasabella heterouncinata, a pest of cultured abalone
- Authors: Simon, Carol Anne
- Date: 2005
- Subjects: Polychaeta -- Physiology Sabellidae -- Physiology Abalones -- Diseases Polychaeta -- Reproduction Sabellidae -- Reproduction Abalone culture -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5336 , http://hdl.handle.net/10962/d1005632
- Description: Terebrasabella heterouncinata is a small K-selected sabellid polychaete. It is a simultaneous hermaphrodite with a semi-continuous mode of reproduction, producing relatively few large eggs that are brooded within the parental burrow until the larvae emerge, to settle on the growing edge of the abalone shell. Despite its low fecundity, this worm has become problematic on abalone farms in South Africa. The present study was conducted to gain an understanding of the life history patterns of T. helerouncinata to determine how they contributed to the success of these worms under altered conditions. This study demonstrated that conditions prevalent on abalone farms were conducive to enhancing the reproductive success of this worm, and suggests that larger, more fecund worms may have been selected for in the decade that these worms have been present on the farms. Increased nutrient availability, and possibly the increased stability of the farm environment relative to its natural environment, has led to a 1.5-fold increase in the average size of the worms. Body size was found to be positively correlated with brood size, and this resulted in worms on farms brooding 3 to 4.5 times more offspring at a time than worms from wild abalone. The ability to increase the number of eggs produced at a time may have been limited by the fact that these worms have only two ovaries. Thus, the increase in fecundity may have been related primarily to the increase in the rate at which the eggs were laid by the worms on the farms, and the increase in the coelomic space available for the storage of these rapidly developing eggs. The ability to increase the rate at which oocytes develop may be related to the vitellogenic mechanisms employed by these worms. Vitellogenic oocytes are able to incorporate high molecular weight yolk precursors from the surrounding coelomic fluid through endocytotic activity. This may allow the oocytes to increase the rate at which they incorporate yolk material under conditions of nutrient enrichment. The increase in fecundity did not occur at the expense of offspring size and, presumably, quality. The increased reproductive output on the farms was compounded by a proportionate increase in the number of reproducing worms within the population. In addition, these worms are long-lived (worms from farmed abalone reached a maximum age of approximately 40 months) and exhibit negligible senescence. Thus, their reproductive output did not change significantly with an increase in age. Furthermore, the proportion of the reproductive worms did not decrease with an increase in age. Thus, within the age range tested, worms of all ages have the potential to make equal contributions to population growth. While diet and abalone stocking density could not be identified as having a significant effect on reproductive output and infestation rate under intensive culture conditions, it was demonstrated that in a naïve abalone population, the total intensity of infestation increased exponentially with time. This increase may be a consequence of an increase in fertilisation success. These worms continuously produce entaquasperm that are released into the water column. The sperm are collected by other individuals that then store the sperm in a single spermatheca. The ability to store sperm relieves individuals of a dependence on the synchronisation of spawning of eggs and sperm. As the population size and density increases, there could be more individuals releasing sperm into the water column, resulting in a continuous supply of sperm. The increased production of eggs would therefore not be constrained by a lack of sperm. The stored sperm are released into the brood chamber to fertilise eggs as they are laid, and this would probably increase the fertilisation success in the species. This study also provides evidence to suggest that reproduction in this worm has a seasonal component. Future studies should concentrate on measuring fertilisation success in greater detail, measuring the effect of season on reproduction, determining whether there are genetic differences between worms on farmed and wild abalone and determining whether wild worms have similar life-spans and age-related fecundity as worms on farms.
- Full Text:
- Date Issued: 2005
- Authors: Simon, Carol Anne
- Date: 2005
- Subjects: Polychaeta -- Physiology Sabellidae -- Physiology Abalones -- Diseases Polychaeta -- Reproduction Sabellidae -- Reproduction Abalone culture -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5336 , http://hdl.handle.net/10962/d1005632
- Description: Terebrasabella heterouncinata is a small K-selected sabellid polychaete. It is a simultaneous hermaphrodite with a semi-continuous mode of reproduction, producing relatively few large eggs that are brooded within the parental burrow until the larvae emerge, to settle on the growing edge of the abalone shell. Despite its low fecundity, this worm has become problematic on abalone farms in South Africa. The present study was conducted to gain an understanding of the life history patterns of T. helerouncinata to determine how they contributed to the success of these worms under altered conditions. This study demonstrated that conditions prevalent on abalone farms were conducive to enhancing the reproductive success of this worm, and suggests that larger, more fecund worms may have been selected for in the decade that these worms have been present on the farms. Increased nutrient availability, and possibly the increased stability of the farm environment relative to its natural environment, has led to a 1.5-fold increase in the average size of the worms. Body size was found to be positively correlated with brood size, and this resulted in worms on farms brooding 3 to 4.5 times more offspring at a time than worms from wild abalone. The ability to increase the number of eggs produced at a time may have been limited by the fact that these worms have only two ovaries. Thus, the increase in fecundity may have been related primarily to the increase in the rate at which the eggs were laid by the worms on the farms, and the increase in the coelomic space available for the storage of these rapidly developing eggs. The ability to increase the rate at which oocytes develop may be related to the vitellogenic mechanisms employed by these worms. Vitellogenic oocytes are able to incorporate high molecular weight yolk precursors from the surrounding coelomic fluid through endocytotic activity. This may allow the oocytes to increase the rate at which they incorporate yolk material under conditions of nutrient enrichment. The increase in fecundity did not occur at the expense of offspring size and, presumably, quality. The increased reproductive output on the farms was compounded by a proportionate increase in the number of reproducing worms within the population. In addition, these worms are long-lived (worms from farmed abalone reached a maximum age of approximately 40 months) and exhibit negligible senescence. Thus, their reproductive output did not change significantly with an increase in age. Furthermore, the proportion of the reproductive worms did not decrease with an increase in age. Thus, within the age range tested, worms of all ages have the potential to make equal contributions to population growth. While diet and abalone stocking density could not be identified as having a significant effect on reproductive output and infestation rate under intensive culture conditions, it was demonstrated that in a naïve abalone population, the total intensity of infestation increased exponentially with time. This increase may be a consequence of an increase in fertilisation success. These worms continuously produce entaquasperm that are released into the water column. The sperm are collected by other individuals that then store the sperm in a single spermatheca. The ability to store sperm relieves individuals of a dependence on the synchronisation of spawning of eggs and sperm. As the population size and density increases, there could be more individuals releasing sperm into the water column, resulting in a continuous supply of sperm. The increased production of eggs would therefore not be constrained by a lack of sperm. The stored sperm are released into the brood chamber to fertilise eggs as they are laid, and this would probably increase the fertilisation success in the species. This study also provides evidence to suggest that reproduction in this worm has a seasonal component. Future studies should concentrate on measuring fertilisation success in greater detail, measuring the effect of season on reproduction, determining whether there are genetic differences between worms on farmed and wild abalone and determining whether wild worms have similar life-spans and age-related fecundity as worms on farms.
- Full Text:
- Date Issued: 2005
The potential of claywork to facilitate the integration of the self in psychotherapy with an adult survivor of childhood trauma: a Jungian perspective
- Authors: Smuts, Tanja
- Date: 2005
- Subjects: Jung, C G (Carl Gustav), 1875-1961 , Art therapy , Psychotherapy , Child psychotherapy , Adult child abuse victims -- Rehabilitation , Self , Modeling -- Therapeutic use
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3062 , http://hdl.handle.net/10962/d1002571 , Jung, C G (Carl Gustav), 1875-1961 , Art therapy , Psychotherapy , Child psychotherapy , Adult child abuse victims -- Rehabilitation , Self , Modeling -- Therapeutic use
- Description: The aim of this thesis was to explore the experience of “self” within the clinical context of adult survivors of childhood trauma. Childhood trauma in this study referred to a range of childhood experiences of emotional and physical assault, including encounters with various kinds of abuse and neglect. The focus was on the experience of a sense of disintegration and dislocation, associated to aspects of self being in conflict. This was explored from theoretical perspectives of Jungian analytic psychology, as well as art therapy.Answers were sought to the questions of how claywork, as a form of art therapy, may facilitate the integration of the self and contribute to the development of a healing dialogue with feared and hated aspects of self. Grounded in Jungian theory, integration of the self was conceptualised as a movement towards “differentiating wholeness”. The study took the form of a phenomenological-hermeneutic case study. One participant’s experience of making and discussing a clay sculpture in a therapeutic setting according to Edwards’ method, was analysed thematically. It was concluded that the potential of claywork in therapy to facilitate the integration of the self is related to three aspects. Firstly, claywork in therapy may promote a concrete personification of feared and hated aspects of self, which may enable the maker to view these aspects from a distance, and learn to understand them in a new way. Secondly, claywork in therapy may facilitate the safe ‘unearthing’ of repressed feelings as well as hidden aspects of the personality. Thirdly, claywork’s potential to mediate symbolic functioning was shown to be an important aspect of the integration process. These findings confirmed and extended existing theory regarding the usefulness of claywork in psychotherapy with adult survivors of childhood trauma. Brief recommendations for future research were provided.
- Full Text:
- Date Issued: 2005
- Authors: Smuts, Tanja
- Date: 2005
- Subjects: Jung, C G (Carl Gustav), 1875-1961 , Art therapy , Psychotherapy , Child psychotherapy , Adult child abuse victims -- Rehabilitation , Self , Modeling -- Therapeutic use
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3062 , http://hdl.handle.net/10962/d1002571 , Jung, C G (Carl Gustav), 1875-1961 , Art therapy , Psychotherapy , Child psychotherapy , Adult child abuse victims -- Rehabilitation , Self , Modeling -- Therapeutic use
- Description: The aim of this thesis was to explore the experience of “self” within the clinical context of adult survivors of childhood trauma. Childhood trauma in this study referred to a range of childhood experiences of emotional and physical assault, including encounters with various kinds of abuse and neglect. The focus was on the experience of a sense of disintegration and dislocation, associated to aspects of self being in conflict. This was explored from theoretical perspectives of Jungian analytic psychology, as well as art therapy.Answers were sought to the questions of how claywork, as a form of art therapy, may facilitate the integration of the self and contribute to the development of a healing dialogue with feared and hated aspects of self. Grounded in Jungian theory, integration of the self was conceptualised as a movement towards “differentiating wholeness”. The study took the form of a phenomenological-hermeneutic case study. One participant’s experience of making and discussing a clay sculpture in a therapeutic setting according to Edwards’ method, was analysed thematically. It was concluded that the potential of claywork in therapy to facilitate the integration of the self is related to three aspects. Firstly, claywork in therapy may promote a concrete personification of feared and hated aspects of self, which may enable the maker to view these aspects from a distance, and learn to understand them in a new way. Secondly, claywork in therapy may facilitate the safe ‘unearthing’ of repressed feelings as well as hidden aspects of the personality. Thirdly, claywork’s potential to mediate symbolic functioning was shown to be an important aspect of the integration process. These findings confirmed and extended existing theory regarding the usefulness of claywork in psychotherapy with adult survivors of childhood trauma. Brief recommendations for future research were provided.
- Full Text:
- Date Issued: 2005