The post-secondary education system: Towards policy formulation for equality and development
- Authors: Badat, Saleem
- Date: 2014
- Language: English
- Type: Text
- Identifier: vital:7861 , http://hdl.handle.net/10962/d1016410
- Full Text:
- Date Issued: 2014
- Authors: Badat, Saleem
- Date: 2014
- Language: English
- Type: Text
- Identifier: vital:7861 , http://hdl.handle.net/10962/d1016410
- Full Text:
- Date Issued: 2014
Kedibone
- Authors: Mokae, Sabata Paul
- Date: 2014
- Subjects: Creative writing (Higher education) -- Research -- South Africa , Creative writing -- Fiction , South African fiction (English) -- Study and teaching (Higher) , South African fiction (English) -- 21st century
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6000 , http://hdl.handle.net/10962/d1020883
- Description: A young woman from a rural village near Kimberley is killed by her husband in a fit of jealousy. Her illiterate mother is summoned to the hospital to authorize the removal of vital organs – eyes, liver, kidney and heart – for organ donation. But some members of the family feel that their child should not be buried with parts of her body missing. Thus begins a story that changes the lives of many people, both black and white, over the following twenty years.
- Full Text:
- Date Issued: 2014
- Authors: Mokae, Sabata Paul
- Date: 2014
- Subjects: Creative writing (Higher education) -- Research -- South Africa , Creative writing -- Fiction , South African fiction (English) -- Study and teaching (Higher) , South African fiction (English) -- 21st century
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6000 , http://hdl.handle.net/10962/d1020883
- Description: A young woman from a rural village near Kimberley is killed by her husband in a fit of jealousy. Her illiterate mother is summoned to the hospital to authorize the removal of vital organs – eyes, liver, kidney and heart – for organ donation. But some members of the family feel that their child should not be buried with parts of her body missing. Thus begins a story that changes the lives of many people, both black and white, over the following twenty years.
- Full Text:
- Date Issued: 2014
A needs-ICTD strategy alignment framework foundation for the measurement of ICTD impact
- Authors: Baduza, Gugulethu Qhawekazi
- Date: 2014
- Subjects: Siyakhula Living Lab SAP Research Needs assessment -- Information technology -- Social aspects -- South Africa -- Case studies Information technology -- South Africa -- Management -- Case studies Information technology -- Social aspects -- South Africa Rural development -- Economic aspects -- South Africa Rural development -- South Africa -- Sociological aspects Rural development projects -- South Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1155 , http://hdl.handle.net/10962/d1011116
- Description: Many Information and Communication Technologies for Development (ICTD) projects are established with the overall aim of positively developing the communities they are implemented in. However, the solutions that are provided are often commonly developed without the needs of these communities being sufficiently investigated beforehand. As a result the ICTD strategy of the project ends up not well aligned with the aims and targets of the needs of the community. As a result of this, an appropriate programme theory for the project and relevant impact indicators fail to be adequately developed. Consequently, when an impact assessment is conducted it is often found that the intended effects are not directly linked to the needs of the community or what the community had hoped to gain from the ICTD initiative. The purpose of this research serves to develop a needs-ICTD strategy alignment foundation that supports the identification and formulation of impact assessment indicators. Through this research, a framework is developed to support the alignment of ICTD strategy, the development and the promotion of contextual needs of rural communities and other frequently marginalized areas. The Needs-ICTD strategy alignment framework is composed of eight main components that describe the process that can be used to align ICTD strategy with community needs. These components include: collaboration between the internal and external stakeholders, the development of the community, conducting baseline studies, the needs assessment, the ICTD strategy, linking of the needs-ICTD strategy, and lastly the identification of impact indicators. An interpretive research approach is used to explore and inform the framework through a multi-case study investigation of the Siyakhula Living Lab and two projects in the Systems Application Products (SAP) Living Lab. Two main case study questions drive the exploration of the framework, that being: 1) How are the needs of the community elicited and how is the ICTD strategy aligned to the needs of the community? 2) And, why were the selected approaches chosen for aligning the needs of the community and ICTD strategy? Data for this research was collected qualitatively through interviews, document analysis and participant observation. Key findings indicate that the involvement of internal (local) stakeholders in the development and alignment of ICTD strategy to the needs of the community is still lacking. As a consequence, many community members end up not fully understanding the project objectives and how these objectives aims are to be achieved. The research also finds that ‘solution specific’ projects also fail communities as they focus specifically on one target group and repeatedly fail to assist the community holistically in supporting their information and their community development needs.
- Full Text:
- Date Issued: 2014
- Authors: Baduza, Gugulethu Qhawekazi
- Date: 2014
- Subjects: Siyakhula Living Lab SAP Research Needs assessment -- Information technology -- Social aspects -- South Africa -- Case studies Information technology -- South Africa -- Management -- Case studies Information technology -- Social aspects -- South Africa Rural development -- Economic aspects -- South Africa Rural development -- South Africa -- Sociological aspects Rural development projects -- South Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1155 , http://hdl.handle.net/10962/d1011116
- Description: Many Information and Communication Technologies for Development (ICTD) projects are established with the overall aim of positively developing the communities they are implemented in. However, the solutions that are provided are often commonly developed without the needs of these communities being sufficiently investigated beforehand. As a result the ICTD strategy of the project ends up not well aligned with the aims and targets of the needs of the community. As a result of this, an appropriate programme theory for the project and relevant impact indicators fail to be adequately developed. Consequently, when an impact assessment is conducted it is often found that the intended effects are not directly linked to the needs of the community or what the community had hoped to gain from the ICTD initiative. The purpose of this research serves to develop a needs-ICTD strategy alignment foundation that supports the identification and formulation of impact assessment indicators. Through this research, a framework is developed to support the alignment of ICTD strategy, the development and the promotion of contextual needs of rural communities and other frequently marginalized areas. The Needs-ICTD strategy alignment framework is composed of eight main components that describe the process that can be used to align ICTD strategy with community needs. These components include: collaboration between the internal and external stakeholders, the development of the community, conducting baseline studies, the needs assessment, the ICTD strategy, linking of the needs-ICTD strategy, and lastly the identification of impact indicators. An interpretive research approach is used to explore and inform the framework through a multi-case study investigation of the Siyakhula Living Lab and two projects in the Systems Application Products (SAP) Living Lab. Two main case study questions drive the exploration of the framework, that being: 1) How are the needs of the community elicited and how is the ICTD strategy aligned to the needs of the community? 2) And, why were the selected approaches chosen for aligning the needs of the community and ICTD strategy? Data for this research was collected qualitatively through interviews, document analysis and participant observation. Key findings indicate that the involvement of internal (local) stakeholders in the development and alignment of ICTD strategy to the needs of the community is still lacking. As a consequence, many community members end up not fully understanding the project objectives and how these objectives aims are to be achieved. The research also finds that ‘solution specific’ projects also fail communities as they focus specifically on one target group and repeatedly fail to assist the community holistically in supporting their information and their community development needs.
- Full Text:
- Date Issued: 2014
"Totally unacceptable" : representations of homosexuality in South African public discourse
- Mutambanengwe, Simbarashe Abel
- Authors: Mutambanengwe, Simbarashe Abel
- Date: 2014
- Subjects: Independent Online , Homosexuality -- South Africa , Sexual minorities in mass media -- South Africa , Mass media and gays -- South Africa , Homophobia -- Press coverage -- South Africa , Electronic newspapers -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2882 , http://hdl.handle.net/10962/d1013259
- Description: The 1996 Constitution of South Africa is ranked as one of the most liberal and democratic constitutions in the world. The right to freedom of sexual orientation, equality and the freedom of association amongst other rights is in its Bill of Rights and are thus inherently assured and protected in post- apartheid, democratic South Africa. However, the Lesbian Gay Bisexual and Transgender (LGBT) community continue to face discrimination and prejudice despite this newly established constitutional order. The present study is interested in how, in the light of the equality clause in the South African constitution, homosexuality is represented and constructed in the South African media. The thesis examines representations of homosexuality between the years 1999-2013 in articles collected from the Independent Online media site which incorporates 30 newspapers. The approach focuses on the topics, overall news report schemata, local meanings, style and rhetoric of the news reports. The results of the study show that negative attitudes towards homosexuality are framed in three main ways: homosexuality is represented as "unAfrican"; "ungodly" and "unnatural". I argue that rather than extreme forms of violence (such as "corrective rape" and murder) directed against LGBT citizens being interpreted as the aberrant behaviour of a few, these need to be understood in the context of the circulation of the above justificatory narratives.
- Full Text:
- Date Issued: 2014
- Authors: Mutambanengwe, Simbarashe Abel
- Date: 2014
- Subjects: Independent Online , Homosexuality -- South Africa , Sexual minorities in mass media -- South Africa , Mass media and gays -- South Africa , Homophobia -- Press coverage -- South Africa , Electronic newspapers -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2882 , http://hdl.handle.net/10962/d1013259
- Description: The 1996 Constitution of South Africa is ranked as one of the most liberal and democratic constitutions in the world. The right to freedom of sexual orientation, equality and the freedom of association amongst other rights is in its Bill of Rights and are thus inherently assured and protected in post- apartheid, democratic South Africa. However, the Lesbian Gay Bisexual and Transgender (LGBT) community continue to face discrimination and prejudice despite this newly established constitutional order. The present study is interested in how, in the light of the equality clause in the South African constitution, homosexuality is represented and constructed in the South African media. The thesis examines representations of homosexuality between the years 1999-2013 in articles collected from the Independent Online media site which incorporates 30 newspapers. The approach focuses on the topics, overall news report schemata, local meanings, style and rhetoric of the news reports. The results of the study show that negative attitudes towards homosexuality are framed in three main ways: homosexuality is represented as "unAfrican"; "ungodly" and "unnatural". I argue that rather than extreme forms of violence (such as "corrective rape" and murder) directed against LGBT citizens being interpreted as the aberrant behaviour of a few, these need to be understood in the context of the circulation of the above justificatory narratives.
- Full Text:
- Date Issued: 2014
Card games and containment : forensic psychiatric patients' experiences of a student-led initiative
- Authors: Higgins, Jane Marie
- Date: 2014
- Subjects: Fort England Hospital Buddy Programme -- Grahamstown -- South Africa , Card games -- Therapeutic use , Mental illness -- Treatment , Mentally ill -- Rehabilitation , Group psychotherapy
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3243 , http://hdl.handle.net/10962/d1013314
- Description: Despite South African forensic psychiatric institutions operating well over capacity, the urgent need for rehabilitation guidelines is neglected through lack of research in this area. This is further compounded by the constrained financial and professional resources available to the sector. The Fort England Hospital Buddy Programme (FEHBP) is a voluntary social and activity-based initiative involving 2 hourly visits between students and male forensic psychiatric patient volunteers. Through the use of Interpretative Phenomenological Analysis (Smith, 1996), the participants’ experience of the programme was further contextualised within their lives pre and post admission. While further exploration through research is required, it appeared that within institutional confines the FEHBP acted in a substitutionary and surrogacy capacity, as a space for the development of social competence. While participants appeared to experience a sense of protectiveness from the programme, the limitations and restrictions are acknowledged as an increased number and variety of social network links would be required for a more sustainable sense of subjective wellbeing to develop. The FEHBP demonstrates the use of non-professional (community involved) interventions within a forensic psychiatric context.
- Full Text:
- Date Issued: 2014
- Authors: Higgins, Jane Marie
- Date: 2014
- Subjects: Fort England Hospital Buddy Programme -- Grahamstown -- South Africa , Card games -- Therapeutic use , Mental illness -- Treatment , Mentally ill -- Rehabilitation , Group psychotherapy
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3243 , http://hdl.handle.net/10962/d1013314
- Description: Despite South African forensic psychiatric institutions operating well over capacity, the urgent need for rehabilitation guidelines is neglected through lack of research in this area. This is further compounded by the constrained financial and professional resources available to the sector. The Fort England Hospital Buddy Programme (FEHBP) is a voluntary social and activity-based initiative involving 2 hourly visits between students and male forensic psychiatric patient volunteers. Through the use of Interpretative Phenomenological Analysis (Smith, 1996), the participants’ experience of the programme was further contextualised within their lives pre and post admission. While further exploration through research is required, it appeared that within institutional confines the FEHBP acted in a substitutionary and surrogacy capacity, as a space for the development of social competence. While participants appeared to experience a sense of protectiveness from the programme, the limitations and restrictions are acknowledged as an increased number and variety of social network links would be required for a more sustainable sense of subjective wellbeing to develop. The FEHBP demonstrates the use of non-professional (community involved) interventions within a forensic psychiatric context.
- Full Text:
- Date Issued: 2014
The demography, spatial ecology and reproductive success of the black rhinoceros, Diceros bicornis, in Hluhluwe-iMfolozi Park, Kwazulu-Natal, South Africa
- Authors: Nhleko, Zoliswa Nombulelo
- Date: 2014
- Subjects: Hluhluwe-iMfolozi Park (South Africa) , Black rhinoceros -- South Africa -- Hluhluwe , Animal populations -- South Africa -- Hluhluwe , Spatial ecology -- South Africa -- Hluhluwe , Black rhinoceros -- Reproduction
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5849 , http://hdl.handle.net/10962/d1011825 , Hluhluwe-iMfolozi Park (South Africa) , Black rhinoceros -- South Africa -- Hluhluwe , Animal populations -- South Africa -- Hluhluwe , Spatial ecology -- South Africa -- Hluhluwe , Black rhinoceros -- Reproduction
- Description: Black rhinoceroses (Diceros bicornis) are endangered; there are only ~5000 individuals remaining worldwide. Consequently, black rhinos are managed as a metapopulation in southern Africa. This means that even though several sub-populations of black rhinos are geographically separated, they are managed as a single population. This is achieved by interchanging genetic material between the sub-populations. The main aim of the metapopulation management approach is to increase black rhino numbers in the sub-region. The Rhino Management Group (RMG), formed in 1989, was tasked with setting scientifically defensible demographic targets for the remaining black rhino sub-populations in southern Africa. In the same year, the RMG set fecundity rate at 25% and stated that each black rhino sub-population should achieve a minimum growth rate of 5% per year. The black rhino population in the Hluhluwe-iMfolozi Park (HiP), KwaZulu-Natal, South Africa, is of international importance as it has historically been a significant donor population for the translocation of black rhinos to establish new subpopulations elsewhere in the sub-region. However, the black rhino population at HiP is performing poorly in terms of reproductive success. My study was initiated to assess the reproductive life-histories of the black rhino population at HiP and to determine whether the population was meeting the demographic targets set by the RMG. I also wished to assess the use of space by black rhinos in HiP as a potential explanation for the observed reproductive output. Detailed life-history information for known black rhinos (n = 120) was used to investigate reproductive success between 1998 and 2012. The mean age at sexual maturity (ASM) was determined as 12 years, which is higher than the ASM found in black rhino populations that are considered to be performing poorly (ASM = ~ 7 years). The intercalving interval (ICI) was three years, nine months; this was also longer than the ICI of most black rhino populations. Further, the 2012 black rhino population at HiP had a growth rate of 6.4%, and the population comprised 60% adults, 23% sub-adults and 13% juveniles. Such demographics are usually observed in stable populations and it is possible that poor habitat quality could be resulting in the delayed ASM and longer ICI’s at HiP. In addition, 5% of the population is removed annually as part of the Black Rhino Range Expansion Program (BRREP) to establish new sub-populations. This could also be negatively influencing the reproductive performance of the HiP population. To determine the home range and habitat use of black rhinos at HiP, field data from 13 individual adult black rhinos (n = 9 females and 4 males) over a two-year period (2011-2012) were analyzed. Black rhino female home ranges were slightly smaller (15.4 ± 12.9 km²) than the home ranges of males (23.3 ±14.0 km²) and both sexes utilized similar habitats in the centre of the park. In addition, both the male and female black rhinos were found closer to rivers and drainage lines than expected. However, the assessment of black rhino space use in HiP was limited by a small sample size, making inferences of the impact of space use on reproductive success difficult. Nevertheless, my study suggests that food availability likely influences the location and sizes of black rhino home ranges at HiP. I believe that my study forms the first step in understanding the factors that influence the reproductive success and habitat use patterns of the current black rhino population at HiP. The information produced by my study should enable park management to identify future research to be conducted at HiP, which will yield more robust data for making informed black rhino management decisions at HiP and elsewhere.
- Full Text:
- Date Issued: 2014
- Authors: Nhleko, Zoliswa Nombulelo
- Date: 2014
- Subjects: Hluhluwe-iMfolozi Park (South Africa) , Black rhinoceros -- South Africa -- Hluhluwe , Animal populations -- South Africa -- Hluhluwe , Spatial ecology -- South Africa -- Hluhluwe , Black rhinoceros -- Reproduction
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5849 , http://hdl.handle.net/10962/d1011825 , Hluhluwe-iMfolozi Park (South Africa) , Black rhinoceros -- South Africa -- Hluhluwe , Animal populations -- South Africa -- Hluhluwe , Spatial ecology -- South Africa -- Hluhluwe , Black rhinoceros -- Reproduction
- Description: Black rhinoceroses (Diceros bicornis) are endangered; there are only ~5000 individuals remaining worldwide. Consequently, black rhinos are managed as a metapopulation in southern Africa. This means that even though several sub-populations of black rhinos are geographically separated, they are managed as a single population. This is achieved by interchanging genetic material between the sub-populations. The main aim of the metapopulation management approach is to increase black rhino numbers in the sub-region. The Rhino Management Group (RMG), formed in 1989, was tasked with setting scientifically defensible demographic targets for the remaining black rhino sub-populations in southern Africa. In the same year, the RMG set fecundity rate at 25% and stated that each black rhino sub-population should achieve a minimum growth rate of 5% per year. The black rhino population in the Hluhluwe-iMfolozi Park (HiP), KwaZulu-Natal, South Africa, is of international importance as it has historically been a significant donor population for the translocation of black rhinos to establish new subpopulations elsewhere in the sub-region. However, the black rhino population at HiP is performing poorly in terms of reproductive success. My study was initiated to assess the reproductive life-histories of the black rhino population at HiP and to determine whether the population was meeting the demographic targets set by the RMG. I also wished to assess the use of space by black rhinos in HiP as a potential explanation for the observed reproductive output. Detailed life-history information for known black rhinos (n = 120) was used to investigate reproductive success between 1998 and 2012. The mean age at sexual maturity (ASM) was determined as 12 years, which is higher than the ASM found in black rhino populations that are considered to be performing poorly (ASM = ~ 7 years). The intercalving interval (ICI) was three years, nine months; this was also longer than the ICI of most black rhino populations. Further, the 2012 black rhino population at HiP had a growth rate of 6.4%, and the population comprised 60% adults, 23% sub-adults and 13% juveniles. Such demographics are usually observed in stable populations and it is possible that poor habitat quality could be resulting in the delayed ASM and longer ICI’s at HiP. In addition, 5% of the population is removed annually as part of the Black Rhino Range Expansion Program (BRREP) to establish new sub-populations. This could also be negatively influencing the reproductive performance of the HiP population. To determine the home range and habitat use of black rhinos at HiP, field data from 13 individual adult black rhinos (n = 9 females and 4 males) over a two-year period (2011-2012) were analyzed. Black rhino female home ranges were slightly smaller (15.4 ± 12.9 km²) than the home ranges of males (23.3 ±14.0 km²) and both sexes utilized similar habitats in the centre of the park. In addition, both the male and female black rhinos were found closer to rivers and drainage lines than expected. However, the assessment of black rhino space use in HiP was limited by a small sample size, making inferences of the impact of space use on reproductive success difficult. Nevertheless, my study suggests that food availability likely influences the location and sizes of black rhino home ranges at HiP. I believe that my study forms the first step in understanding the factors that influence the reproductive success and habitat use patterns of the current black rhino population at HiP. The information produced by my study should enable park management to identify future research to be conducted at HiP, which will yield more robust data for making informed black rhino management decisions at HiP and elsewhere.
- Full Text:
- Date Issued: 2014
Purification and characterization of TbHsp70.c, a novel Hsp70 from Trypanosoma brucei
- Authors: Burger, Adélle
- Date: 2014
- Subjects: African trypanosomiasis -- Research Heat shock proteins -- Research Trypanosoma brucei -- Research Mycobacterial diseases -- Research -- Africa Parasitic diseases -- Africa -- Prevention Parasites -- Physiology Developing countries -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4105 , http://hdl.handle.net/10962/d1011618
- Description: One of Africa’s neglected tropical diseases, African Trypanosomiasis, is not only fatal but also has a crippling impact on economic development. Heat shock proteins play a wide range of roles in the cell and they are required to assist the parasite as it moves from a cold blooded insect vector to a warm blooded mammalian host. The expression of heat shock proteins increases during these heat shock conditions, and this is considered to play a role in differentiation of these vector-borne parasites. Heat shock protein 70 (Hsp70) is an important molecular chaperone that is involved in protein homeostasis, Hsp40 acts as a co-chaperone and stimulates its intrinsically weak ATPase activity. In silico analysis of the T. brucei genome has revealed the existence of 12 Hsp70 proteins and 65 Hsp40 proteins to date. A novel Hsp70, TbHsp70.c, was recently identified in T. brucei. Different from the prototypical Hsp70, TbHsp70.c contains an acidic substrate binding domain and lacks the C-terminal EEVD motif. By implication the substrate range and mechanism by which the substrates are recognized may be novel. The ability of a Type I Hsp40, Tbj2, to function as a co-chaperone of TbHsp70.c was investigated. The main objective of this study was to biochemically characterize TbHsp70.c and its partnership with Tbj2 to further enhance our knowledge of parasite biology. TbHsp70.c and Tbj2 were heterologously expressed and purified and both proteins displayed chaperone activities in their ability to suppress aggregation of thermolabile MDH. TbHsp70.c also suppressed aggregation of rhodanese. ATPase assays revealed that the ATPase activity of TbHsp70.c was stimulated by Tbj2. The targeted inhibition of the function of heat shock proteins is emerging as a tool to combat disease. The small molecule modulators quercetin and methylene blue are known to inhibit the ATPase activity of Hsp70. However, methylene blue did not significantly inhibit the ATPase activity of TbHsp70.c; while quercetin, did inhibit the ATPase activity. In vivo heat stress experiments indicated an up-regulation of the expression levels of TbHsp70.c. RNA interference studies showed partial knockdown of TbHsp70.c with no detrimental effect on the parasite. Fluorescence microscopy studies of TbHsp70.c showed a probable cytoplasmic subcellular localization. In this study both TbHsp70.c and Tbj2 demonstrated chaperone activity and Tbj2 possibly functions as a co-chaperone of TbHsp70.c.
- Full Text:
- Date Issued: 2014
- Authors: Burger, Adélle
- Date: 2014
- Subjects: African trypanosomiasis -- Research Heat shock proteins -- Research Trypanosoma brucei -- Research Mycobacterial diseases -- Research -- Africa Parasitic diseases -- Africa -- Prevention Parasites -- Physiology Developing countries -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4105 , http://hdl.handle.net/10962/d1011618
- Description: One of Africa’s neglected tropical diseases, African Trypanosomiasis, is not only fatal but also has a crippling impact on economic development. Heat shock proteins play a wide range of roles in the cell and they are required to assist the parasite as it moves from a cold blooded insect vector to a warm blooded mammalian host. The expression of heat shock proteins increases during these heat shock conditions, and this is considered to play a role in differentiation of these vector-borne parasites. Heat shock protein 70 (Hsp70) is an important molecular chaperone that is involved in protein homeostasis, Hsp40 acts as a co-chaperone and stimulates its intrinsically weak ATPase activity. In silico analysis of the T. brucei genome has revealed the existence of 12 Hsp70 proteins and 65 Hsp40 proteins to date. A novel Hsp70, TbHsp70.c, was recently identified in T. brucei. Different from the prototypical Hsp70, TbHsp70.c contains an acidic substrate binding domain and lacks the C-terminal EEVD motif. By implication the substrate range and mechanism by which the substrates are recognized may be novel. The ability of a Type I Hsp40, Tbj2, to function as a co-chaperone of TbHsp70.c was investigated. The main objective of this study was to biochemically characterize TbHsp70.c and its partnership with Tbj2 to further enhance our knowledge of parasite biology. TbHsp70.c and Tbj2 were heterologously expressed and purified and both proteins displayed chaperone activities in their ability to suppress aggregation of thermolabile MDH. TbHsp70.c also suppressed aggregation of rhodanese. ATPase assays revealed that the ATPase activity of TbHsp70.c was stimulated by Tbj2. The targeted inhibition of the function of heat shock proteins is emerging as a tool to combat disease. The small molecule modulators quercetin and methylene blue are known to inhibit the ATPase activity of Hsp70. However, methylene blue did not significantly inhibit the ATPase activity of TbHsp70.c; while quercetin, did inhibit the ATPase activity. In vivo heat stress experiments indicated an up-regulation of the expression levels of TbHsp70.c. RNA interference studies showed partial knockdown of TbHsp70.c with no detrimental effect on the parasite. Fluorescence microscopy studies of TbHsp70.c showed a probable cytoplasmic subcellular localization. In this study both TbHsp70.c and Tbj2 demonstrated chaperone activity and Tbj2 possibly functions as a co-chaperone of TbHsp70.c.
- Full Text:
- Date Issued: 2014
Trends in the fish assemblage structure of two South African transition-zone estuaries : can these trends be linked to climate change?
- Authors: Midgley, Craig
- Date: 2014
- Subjects: Estuarine fishes -- South Africa -- Climatic factors , Fish communities -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5368 , http://hdl.handle.net/10962/d1013173
- Description: Changes in the fish communities of the Breede and Mbashe estuaries were investigated in relation to the environmental variables that influence the fish assemblages in these systems. The Breede Estuary (34° 24’21.6”S, 20° 51’ 08.2”E) occurs within the warm-temperate/cool-temperate transition-zone, while the Mbashe Estuary (32o 14' 55.4"S, 28o 54' 03.7"E) falls within the subtropical/warm-temperate transition-zone along the South African coastline. The Breede Estuary was sampled (seine and gill nets) during summer (January-March) over a period of 10 years (2002-2012), while the Mbashe Estuary was sampled (seine net) during spring (October) over a period of three years (2010-2012). The proportion of tropical fish species was higher in the Mbashe Estuary compared to the Breede Estuary, while the Breede Estuary contained a higher proportion of temperate species than the Mbashe Estuary. Although the abundance of individual species in both estuaries varied, the ranking of species in the Breede Estuary was stable over the 10 year period. Multivariate analysis showed that the fish communities within each reach (upper, middle, lower) of the Breede Estuary remained similar, regardless of year. In contrast, species composition in the Mbashe Estuary differed significantly between years mainly due to differences in the river flow regime during the study period. Environmental variables responsible for structuring the fish assemblage in each estuary differed. Salinity significantly impacted the spatial fish assemblage structure of the Breede Estuary, with most of the species recorded being associated with the more saline lower reaches. The abundance of tropical species near their distributional limit in the Breede Estuary appeared to be impacted by sea surface temperature (SST) as peak total abundance of these species coincided with the warmest SST, while the lowest total abundance coincided with the coldest annual SST during the study period. The fish assemblage structure in the Mbashe Estuary was impacted by differing freshwater input prior to sampling, with flooding negatively impacting the mean total abundance, species composition and diversity in this system. The variables that influenced the fish assemblage structure of both transition-zone estuaries will be affected by climate change in the future. These variables will, in turn, determine the composition, abundance and diversity of species within these important estuarine systems. In order to measure these impacts with any degree of understanding, long-term studies on the abiotic and biotic (including the ichthyofauna) features of these estuaries are required to interpret climate change trends.
- Full Text:
- Date Issued: 2014
- Authors: Midgley, Craig
- Date: 2014
- Subjects: Estuarine fishes -- South Africa -- Climatic factors , Fish communities -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5368 , http://hdl.handle.net/10962/d1013173
- Description: Changes in the fish communities of the Breede and Mbashe estuaries were investigated in relation to the environmental variables that influence the fish assemblages in these systems. The Breede Estuary (34° 24’21.6”S, 20° 51’ 08.2”E) occurs within the warm-temperate/cool-temperate transition-zone, while the Mbashe Estuary (32o 14' 55.4"S, 28o 54' 03.7"E) falls within the subtropical/warm-temperate transition-zone along the South African coastline. The Breede Estuary was sampled (seine and gill nets) during summer (January-March) over a period of 10 years (2002-2012), while the Mbashe Estuary was sampled (seine net) during spring (October) over a period of three years (2010-2012). The proportion of tropical fish species was higher in the Mbashe Estuary compared to the Breede Estuary, while the Breede Estuary contained a higher proportion of temperate species than the Mbashe Estuary. Although the abundance of individual species in both estuaries varied, the ranking of species in the Breede Estuary was stable over the 10 year period. Multivariate analysis showed that the fish communities within each reach (upper, middle, lower) of the Breede Estuary remained similar, regardless of year. In contrast, species composition in the Mbashe Estuary differed significantly between years mainly due to differences in the river flow regime during the study period. Environmental variables responsible for structuring the fish assemblage in each estuary differed. Salinity significantly impacted the spatial fish assemblage structure of the Breede Estuary, with most of the species recorded being associated with the more saline lower reaches. The abundance of tropical species near their distributional limit in the Breede Estuary appeared to be impacted by sea surface temperature (SST) as peak total abundance of these species coincided with the warmest SST, while the lowest total abundance coincided with the coldest annual SST during the study period. The fish assemblage structure in the Mbashe Estuary was impacted by differing freshwater input prior to sampling, with flooding negatively impacting the mean total abundance, species composition and diversity in this system. The variables that influenced the fish assemblage structure of both transition-zone estuaries will be affected by climate change in the future. These variables will, in turn, determine the composition, abundance and diversity of species within these important estuarine systems. In order to measure these impacts with any degree of understanding, long-term studies on the abiotic and biotic (including the ichthyofauna) features of these estuaries are required to interpret climate change trends.
- Full Text:
- Date Issued: 2014
Prolegomena to ubuntu and any other future South African philosophy
- Authors: Prinsloo, Aidan Vivian
- Date: 2014
- Subjects: Ubuntu (Philosophy) , Political science -- Philosophy , Philosophy, African , Social values -- South Africa , Social values -- South Africa -- Philosophy , Place (Philosophy)
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2747 , http://hdl.handle.net/10962/d1013092
- Description: In this thesis I consider ubuntu as a metonym for the particularly African features of South African philosophy. Given that Mbembe critiques African philosophy in general as having failed because it has been subsumed under two unreflective political movements in African thought, I consider whether or not the concept of ubuntu escapes his critique. After developing criteria for measuring the success of any philosophical concept, I conclude that ubuntu is unsuccessful. I then identify the political constraints placed on ubuntu that lead to its failure. These constraints arise from having to validate Africa as a place of intellectual worth. Considering the role of place in these constraints, I argue that a far more productive approach to ubuntu (and South African philosophy in general) is to explicitly incorporate this place into our philosophical project. I use the conceptual framework developed by Bruce Janz to provide a systematic account of place that can be used in formulating South African philosophy. I add to Janz, arguing that philosophy is a response to a particular feature of place: the mystery. By incorporating place into ubuntu, I am able to start developing a philosophical concept which can fulfil the political constraints placed on ubuntu without sacrificing its philosophical integrity. I suggest that ubuntu remains an interesting concept primarily because it promises to respond to the fragmentation of the South African place. I conclude by arguing that ubuntu should be used as the basis for a civic religion which responds to the fragmentation of the South African place. This civic religion will give rise to a significantly distinct philosophical tradition which should not succumb to Mbembe’s critique.
- Full Text:
- Date Issued: 2014
- Authors: Prinsloo, Aidan Vivian
- Date: 2014
- Subjects: Ubuntu (Philosophy) , Political science -- Philosophy , Philosophy, African , Social values -- South Africa , Social values -- South Africa -- Philosophy , Place (Philosophy)
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2747 , http://hdl.handle.net/10962/d1013092
- Description: In this thesis I consider ubuntu as a metonym for the particularly African features of South African philosophy. Given that Mbembe critiques African philosophy in general as having failed because it has been subsumed under two unreflective political movements in African thought, I consider whether or not the concept of ubuntu escapes his critique. After developing criteria for measuring the success of any philosophical concept, I conclude that ubuntu is unsuccessful. I then identify the political constraints placed on ubuntu that lead to its failure. These constraints arise from having to validate Africa as a place of intellectual worth. Considering the role of place in these constraints, I argue that a far more productive approach to ubuntu (and South African philosophy in general) is to explicitly incorporate this place into our philosophical project. I use the conceptual framework developed by Bruce Janz to provide a systematic account of place that can be used in formulating South African philosophy. I add to Janz, arguing that philosophy is a response to a particular feature of place: the mystery. By incorporating place into ubuntu, I am able to start developing a philosophical concept which can fulfil the political constraints placed on ubuntu without sacrificing its philosophical integrity. I suggest that ubuntu remains an interesting concept primarily because it promises to respond to the fragmentation of the South African place. I conclude by arguing that ubuntu should be used as the basis for a civic religion which responds to the fragmentation of the South African place. This civic religion will give rise to a significantly distinct philosophical tradition which should not succumb to Mbembe’s critique.
- Full Text:
- Date Issued: 2014
Factors influencing ecological sustainability in the ostrich industry in the Little Karoo, South Africa
- Authors: Wheeler, Anita
- Date: 2014
- Subjects: Ostrich farms -- South Africa -- Little Karoo , Land degradation -- South Africa -- Little Karoo , Land use -- South Africa -- Little Karoo , Land degradation -- Environmental aspects -- South Africa -- Little Karoo , Conservation of natural resources -- South Africa -- Little Karoo , Sustainable development -- South Africa -- Little Karoo , Sustainable agriculture -- South Africa -- Little Karoo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4259 , http://hdl.handle.net/10962/d1011741 , Ostrich farms -- South Africa -- Little Karoo , Land degradation -- South Africa -- Little Karoo , Land use -- South Africa -- Little Karoo , Land degradation -- Environmental aspects -- South Africa -- Little Karoo , Conservation of natural resources -- South Africa -- Little Karoo , Sustainable development -- South Africa -- Little Karoo , Sustainable agriculture -- South Africa -- Little Karoo
- Description: The Little Karoo, situated in the Succulent Karoo biome of South Africa, has been heavily transformed by land use, and only 8.6% of the remaining natural vegetation is considered to be intact. There is ample evidence that the main cause of degradation is the overstocking of ostriches, an industry that has been the major economic driver of the area for more than 150 years. The ostrich stocking rate currently recommended is 22 ha.ostrich⁻¹. A literature review was used to examine the evidence, assumptions and rationale on which recommended and actually implemented stocking rates for ostriches are based. No experimental evidence using ostriches was found that supported the recommended stocking rate as either ecologically or economically sustainable. From the literature, there appears to be a wide gap between what are considered to be economically and ecologically sustainable stocking rates, with the implication that these two aims may be impossible to reconcile when practicing ostrich farming on natural veld. A comparison of recommended with actual stocking rates among land managers in the Little Karoo showed that all land managers far exceeded the recommended agricultural stocking rate of 22.8 ha.ostrich⁻¹. However, the stocking rates reported by land managers to the South African Ostrich Business Chamber were found to accurately reflect actual numbers determined when whole flocks had to be slaughtered after an outbreak of avian influenza. The recommended stocking rate appeared to be irrelevant to ostrich flock breeders and there appeared to be a trade-off between profitable ostrich farming and sustainable land-use practices. This study also investigated the attitudes and behaviour of ostrich farmers. It was found that environmental attitude is most likely the most important characteristic of a land manager to ensure positive conservation behaviour. This characteristic was most prominent in younger land managers with larger farms. The last component of the study integrated the context and complexity of the long term social, economic and ecological sustainability of this industry through the development of a logic model. The results showed a general lack of linkages between industry elements which impact on achieving sustainability targets. Greater collaboration between industry role-players, organized agriculture and conservation organizations is required to find a balance between utilization and conservation in the ostrich industry.
- Full Text:
- Date Issued: 2014
- Authors: Wheeler, Anita
- Date: 2014
- Subjects: Ostrich farms -- South Africa -- Little Karoo , Land degradation -- South Africa -- Little Karoo , Land use -- South Africa -- Little Karoo , Land degradation -- Environmental aspects -- South Africa -- Little Karoo , Conservation of natural resources -- South Africa -- Little Karoo , Sustainable development -- South Africa -- Little Karoo , Sustainable agriculture -- South Africa -- Little Karoo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4259 , http://hdl.handle.net/10962/d1011741 , Ostrich farms -- South Africa -- Little Karoo , Land degradation -- South Africa -- Little Karoo , Land use -- South Africa -- Little Karoo , Land degradation -- Environmental aspects -- South Africa -- Little Karoo , Conservation of natural resources -- South Africa -- Little Karoo , Sustainable development -- South Africa -- Little Karoo , Sustainable agriculture -- South Africa -- Little Karoo
- Description: The Little Karoo, situated in the Succulent Karoo biome of South Africa, has been heavily transformed by land use, and only 8.6% of the remaining natural vegetation is considered to be intact. There is ample evidence that the main cause of degradation is the overstocking of ostriches, an industry that has been the major economic driver of the area for more than 150 years. The ostrich stocking rate currently recommended is 22 ha.ostrich⁻¹. A literature review was used to examine the evidence, assumptions and rationale on which recommended and actually implemented stocking rates for ostriches are based. No experimental evidence using ostriches was found that supported the recommended stocking rate as either ecologically or economically sustainable. From the literature, there appears to be a wide gap between what are considered to be economically and ecologically sustainable stocking rates, with the implication that these two aims may be impossible to reconcile when practicing ostrich farming on natural veld. A comparison of recommended with actual stocking rates among land managers in the Little Karoo showed that all land managers far exceeded the recommended agricultural stocking rate of 22.8 ha.ostrich⁻¹. However, the stocking rates reported by land managers to the South African Ostrich Business Chamber were found to accurately reflect actual numbers determined when whole flocks had to be slaughtered after an outbreak of avian influenza. The recommended stocking rate appeared to be irrelevant to ostrich flock breeders and there appeared to be a trade-off between profitable ostrich farming and sustainable land-use practices. This study also investigated the attitudes and behaviour of ostrich farmers. It was found that environmental attitude is most likely the most important characteristic of a land manager to ensure positive conservation behaviour. This characteristic was most prominent in younger land managers with larger farms. The last component of the study integrated the context and complexity of the long term social, economic and ecological sustainability of this industry through the development of a logic model. The results showed a general lack of linkages between industry elements which impact on achieving sustainability targets. Greater collaboration between industry role-players, organized agriculture and conservation organizations is required to find a balance between utilization and conservation in the ostrich industry.
- Full Text:
- Date Issued: 2014
Petrography, geochemistry and origin of atypical sedimentary-igneous contact relationships at the base of the Hotazel Formation around Middelplaats, Northern Cape Province, RSA
- Authors: Terracin, Matthew Theodore
- Date: 2014
- Subjects: Petrology -- South Africa , Geochemistry -- South Africa , Igneous rocks -- South Africa , Manganese ores -- South Africa , Manganese ores -- Geology -- South Africa , Metasomatism (Mineralogy) , Potassium
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5059 , http://hdl.handle.net/10962/d1012985
- Description: In the Middelplaats mine area of the Kalahari manganese field, two drill holes (MP53 and MP54) intersected anomalously high-grade manganese ore sitting stratigraphically just above an igneous body (likely a dike or sill). Manganese ore located within approximate 5 meters of the contact with the underlying igneous rocks has been substantially metasomatically upgraded from 25 percent manganese, to over 40 percent whilst the dominant manganese species within the ore has been altered to hausmannite. This report demonstrates the metasomatic alteration is related to devolatilization (removal and/or remobilization of H₂O, CO₂ and CaO) due to contact metamorphism caused by the underlying igneous rocks. The Middelplaats mine is situated in the southwest corner of the Kalahari manganese field where the paleo basin shallows out and ends. Within the mine area, several stratigraphic units pinch out or are truncated by the side of the basin. This pinching out of lithological formations has led to the underlying Ongeluk Formation being in contact with the much younger units of the Hotazel Formation. Therefore, geochemical investigation into the nature and source of the igneous rocks was also undertaken to see if the rocks from the two drill holes were related to one another and/or the underlying Ongeluk Formation. Results of these geochemical studies have demonstrated that the Middelplaats igneous rocks (dolerites) from the two drill holes (MP53 and MP54) share a co-genetic source region. There is also reasonable geochemical evidence that the source region of the Middelplaats igneous rocks was substantially similar to the source region of the Ongeluk Formation. This may indicate that the source region of the Ongeluk Formation was reactivated at some later stage resulting in the emplacement of doleritic dikes or sills in the Middelplaats mine area. The Middelplaats igneous rocks were also found to have undergone a slight but pervasive potassic alteration; with most of the original plagioclase feldspar showing some level of replacement by a potassium enriched feldspar. Although no source for this potassic fluid was found, the devolatilization reaction within the manganese ore appears to have released some potassium into the surrounding rocks. This additional potassium may be responsible for some localized potassic alteration.
- Full Text:
- Date Issued: 2014
- Authors: Terracin, Matthew Theodore
- Date: 2014
- Subjects: Petrology -- South Africa , Geochemistry -- South Africa , Igneous rocks -- South Africa , Manganese ores -- South Africa , Manganese ores -- Geology -- South Africa , Metasomatism (Mineralogy) , Potassium
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5059 , http://hdl.handle.net/10962/d1012985
- Description: In the Middelplaats mine area of the Kalahari manganese field, two drill holes (MP53 and MP54) intersected anomalously high-grade manganese ore sitting stratigraphically just above an igneous body (likely a dike or sill). Manganese ore located within approximate 5 meters of the contact with the underlying igneous rocks has been substantially metasomatically upgraded from 25 percent manganese, to over 40 percent whilst the dominant manganese species within the ore has been altered to hausmannite. This report demonstrates the metasomatic alteration is related to devolatilization (removal and/or remobilization of H₂O, CO₂ and CaO) due to contact metamorphism caused by the underlying igneous rocks. The Middelplaats mine is situated in the southwest corner of the Kalahari manganese field where the paleo basin shallows out and ends. Within the mine area, several stratigraphic units pinch out or are truncated by the side of the basin. This pinching out of lithological formations has led to the underlying Ongeluk Formation being in contact with the much younger units of the Hotazel Formation. Therefore, geochemical investigation into the nature and source of the igneous rocks was also undertaken to see if the rocks from the two drill holes were related to one another and/or the underlying Ongeluk Formation. Results of these geochemical studies have demonstrated that the Middelplaats igneous rocks (dolerites) from the two drill holes (MP53 and MP54) share a co-genetic source region. There is also reasonable geochemical evidence that the source region of the Middelplaats igneous rocks was substantially similar to the source region of the Ongeluk Formation. This may indicate that the source region of the Ongeluk Formation was reactivated at some later stage resulting in the emplacement of doleritic dikes or sills in the Middelplaats mine area. The Middelplaats igneous rocks were also found to have undergone a slight but pervasive potassic alteration; with most of the original plagioclase feldspar showing some level of replacement by a potassium enriched feldspar. Although no source for this potassic fluid was found, the devolatilization reaction within the manganese ore appears to have released some potassium into the surrounding rocks. This additional potassium may be responsible for some localized potassic alteration.
- Full Text:
- Date Issued: 2014
An analysis of alternative objective measures of economic performance and social development.
- Authors: Hlanti, Msawenkosi Madoda
- Date: 2014
- Subjects: National income -- Economic aspects -- South Africa -- Evaluation , Economic development -- Social aspects -- South Africa -- Evaluation , Gross domestic product -- South Africa , Sustainable development -- South Africa , Social planning -- South Africa , Economic policy , South Africa -- Social conditions
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1099 , http://hdl.handle.net/10962/d1013144
- Description: The measurement of economic performance and social development has become increasingly important as societies have evolved and become more complex. At present nations do not only seek to improve economic performance but are also compelled to improve social development through improvements in socially and environmentally sustainable initiatives. Traditional measures such as Gross Domestic Product (GDP) which is derived from United Nations’ System of National Accounts (SNA) have been criticised given the inability to adequately account for these social and environmental aspects of social development. Given these perceived deficiencies in the conventional measures, several alternative objective measures have been proposed in an attempt to address these shortcomings. Therefore the primary aim of this study is to analyse, via a literature survey, these alternative objective measures of economic performance and social development. The alternative measures that constitute the survey are the Index of Sustainable Economic Welfare (ISEW), the Genuine Savings (GS), and the United Nations’ Human Development Index (HDI). Upon the completion of the literature survey, sustainable development theory is used to evaluate the extent to which the National Accounts and the alternative objective measures are consistent with Hicksian and Fisherian definitions of income and capital, which embody the concepts of sustainability and sustainable development. The evaluation reveals that the National Accounts neither conform to the Hicksian nor the Fisherian definitions of income, thus could not be viewed as a measure of sustainable income. It is found that the ISEW is consistent with the Fisherian definition of income and is also a partial indicator of sustainable development. The evaluation of the GS measure reveals that it is consistent with the Hicksian definition but not the Fisherian definition. In terms of overall sustainability, it is argued that GS is a partial measure of weak sustainability. The HDI is similar to the National Accounts, in that it is neither consistent with the Hicksian nor the Fisherian definitions of income and is also not a measure of sustainability. In summary, the study demonstrates that despite GDP's shortcomings as a measure of economic performance and social development, currently, there is no alternative approach which simultaneously addresses every flaw in GDP. However, all the alternatives yield a much better approximation of social development than GDP.
- Full Text:
- Date Issued: 2014
- Authors: Hlanti, Msawenkosi Madoda
- Date: 2014
- Subjects: National income -- Economic aspects -- South Africa -- Evaluation , Economic development -- Social aspects -- South Africa -- Evaluation , Gross domestic product -- South Africa , Sustainable development -- South Africa , Social planning -- South Africa , Economic policy , South Africa -- Social conditions
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1099 , http://hdl.handle.net/10962/d1013144
- Description: The measurement of economic performance and social development has become increasingly important as societies have evolved and become more complex. At present nations do not only seek to improve economic performance but are also compelled to improve social development through improvements in socially and environmentally sustainable initiatives. Traditional measures such as Gross Domestic Product (GDP) which is derived from United Nations’ System of National Accounts (SNA) have been criticised given the inability to adequately account for these social and environmental aspects of social development. Given these perceived deficiencies in the conventional measures, several alternative objective measures have been proposed in an attempt to address these shortcomings. Therefore the primary aim of this study is to analyse, via a literature survey, these alternative objective measures of economic performance and social development. The alternative measures that constitute the survey are the Index of Sustainable Economic Welfare (ISEW), the Genuine Savings (GS), and the United Nations’ Human Development Index (HDI). Upon the completion of the literature survey, sustainable development theory is used to evaluate the extent to which the National Accounts and the alternative objective measures are consistent with Hicksian and Fisherian definitions of income and capital, which embody the concepts of sustainability and sustainable development. The evaluation reveals that the National Accounts neither conform to the Hicksian nor the Fisherian definitions of income, thus could not be viewed as a measure of sustainable income. It is found that the ISEW is consistent with the Fisherian definition of income and is also a partial indicator of sustainable development. The evaluation of the GS measure reveals that it is consistent with the Hicksian definition but not the Fisherian definition. In terms of overall sustainability, it is argued that GS is a partial measure of weak sustainability. The HDI is similar to the National Accounts, in that it is neither consistent with the Hicksian nor the Fisherian definitions of income and is also not a measure of sustainability. In summary, the study demonstrates that despite GDP's shortcomings as a measure of economic performance and social development, currently, there is no alternative approach which simultaneously addresses every flaw in GDP. However, all the alternatives yield a much better approximation of social development than GDP.
- Full Text:
- Date Issued: 2014
Analysis of the interaction of Hsp90 with the extracellular matrix protein fibronectin (FN)
- Authors: Hunter, Morgan Campbell
- Date: 2014
- Subjects: Heat shock proteins , Fibronectins , Extracellular matrix proteins , Breast -- Cancer
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4170 , http://hdl.handle.net/10962/d1020960
- Description: Mounting evidence suggests that Hsp90 is present and functionally active in the extracellular space. The biological function of extracellular Hsp90 (eHsp90) remains relatively uncharacterized compared to that of intracellular Hsp90. eHsp90 has been shown to interact with a finite number of extracellular proteins, however, despite the identification of eHsp90 interacting proteins, the function of eHsp90 in these complexes is unknown. Several reports suggest a role for eHsp90α in cell migration and invasion. Reported targets for eHsp90 stimulated cell migration include MMPs, LRP-1, tyrosine kinase receptors and possible others unidentified. Limited studies report a role for eHsp90β. Recently, Hsp90α and Hsp90β were isolated in a complex containing fibronectin (FN) on the surface of MDA-MB-231 breast cancer cells. Herein, we report direct binding of Hsp90α and Hsp90β to FN using a solid phase binding assay and surface plasmon resonance (SPR) spectroscopy. SPR spectroscopy showed that Hsp90β bound the 70 kDa amino-terminal fragment of FN (FN70), but that binding of FN to Hsp90β was not limited to FN70. Confocal microscopy showed regions of colocalization of Hsp90 with extracellular FN matrix fibrils in Hs578T breast cancer cell lines. Treatment of Hs578T breast cancer cells with novobiocin (an Hsp90 inhibitor) and an LRP-1 blocking antibody resulted in a loss of FN matrix and FN endocytosis (novobiocin treated). Addition of exogenous Hsp90β was able to recover such effect after both treatments. FN was shown to colocalize with intracellular LRP-1 in novobiocin treated Hs578T cells. Immunoprecipitation of an LRP-1 containing complex showed the presence of Hsp90 and 70 and 120+ kDa FN fragments. Treatment of Hs578T cells with novobiocin increased the level of FN120+ bound in LRP-1 immunoprecipitate. Exogenous Hsp90β decreased the level of low and high molecular weight FN fragments in a complex with LRP-1, despite the fact that higher levels of lower molecular weight FN fragments were detected in this cell lysate compared to the other treatments. We report FN as a novel interacting protein of eHsp90. Taken together, we provide evidence for a direct role of eHsp90β in FN matrix remodeling. We suggest that Hsp90 plays a direct role in FN matrix dynamics through interaction with FN and LRP-1. The identification of FN as a novel interacting protein of eHsp90 suggests a role for Hsp90 in FN matrix remodeling, which is important for a number of fundamental cellular processes including cell migration and metastasis.
- Full Text:
- Date Issued: 2014
- Authors: Hunter, Morgan Campbell
- Date: 2014
- Subjects: Heat shock proteins , Fibronectins , Extracellular matrix proteins , Breast -- Cancer
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4170 , http://hdl.handle.net/10962/d1020960
- Description: Mounting evidence suggests that Hsp90 is present and functionally active in the extracellular space. The biological function of extracellular Hsp90 (eHsp90) remains relatively uncharacterized compared to that of intracellular Hsp90. eHsp90 has been shown to interact with a finite number of extracellular proteins, however, despite the identification of eHsp90 interacting proteins, the function of eHsp90 in these complexes is unknown. Several reports suggest a role for eHsp90α in cell migration and invasion. Reported targets for eHsp90 stimulated cell migration include MMPs, LRP-1, tyrosine kinase receptors and possible others unidentified. Limited studies report a role for eHsp90β. Recently, Hsp90α and Hsp90β were isolated in a complex containing fibronectin (FN) on the surface of MDA-MB-231 breast cancer cells. Herein, we report direct binding of Hsp90α and Hsp90β to FN using a solid phase binding assay and surface plasmon resonance (SPR) spectroscopy. SPR spectroscopy showed that Hsp90β bound the 70 kDa amino-terminal fragment of FN (FN70), but that binding of FN to Hsp90β was not limited to FN70. Confocal microscopy showed regions of colocalization of Hsp90 with extracellular FN matrix fibrils in Hs578T breast cancer cell lines. Treatment of Hs578T breast cancer cells with novobiocin (an Hsp90 inhibitor) and an LRP-1 blocking antibody resulted in a loss of FN matrix and FN endocytosis (novobiocin treated). Addition of exogenous Hsp90β was able to recover such effect after both treatments. FN was shown to colocalize with intracellular LRP-1 in novobiocin treated Hs578T cells. Immunoprecipitation of an LRP-1 containing complex showed the presence of Hsp90 and 70 and 120+ kDa FN fragments. Treatment of Hs578T cells with novobiocin increased the level of FN120+ bound in LRP-1 immunoprecipitate. Exogenous Hsp90β decreased the level of low and high molecular weight FN fragments in a complex with LRP-1, despite the fact that higher levels of lower molecular weight FN fragments were detected in this cell lysate compared to the other treatments. We report FN as a novel interacting protein of eHsp90. Taken together, we provide evidence for a direct role of eHsp90β in FN matrix remodeling. We suggest that Hsp90 plays a direct role in FN matrix dynamics through interaction with FN and LRP-1. The identification of FN as a novel interacting protein of eHsp90 suggests a role for Hsp90 in FN matrix remodeling, which is important for a number of fundamental cellular processes including cell migration and metastasis.
- Full Text:
- Date Issued: 2014
Invariant optimal control on the three-dimensional semi-Euclidean group: control affine and quadratic Hamilton-Poisson systems
- Authors: Barrett, Dennis Ian
- Date: 2014
- Subjects: Automorphisms , Symmetry (Mathematics) , Lyapunov stability , Geometry, Riemannian , Geometry, Affine , Elliptic functions
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/64805 , vital:28605
- Description: In this thesis we consider invariant control systems and Hamilton-Poisson systems on the three dimensional semi-Euclidean group SE(1,1). We first classify the left-invariant control affine systems (under detached feedback equivalence). We provide a complete list of normal forms, as well as classifying conditions. As a corollary to this classification, we derive controllability criteria for control affine systems on SE(1,1). Secondly, we consider quadratic Hamilton-Poisson systems on the (minus) Lie-Poisson space se(1,1)*. These systems are classified up to an affine isomorphism. Six normal forms are identified for the homogeneous case, whereas sixteen representatives (including several infinite families) are obtained for the inhomogeneous systems. Thereafter we consider the stability and integration of the normal forms obtained. For all homogeneous systems, and a subclass of inhomogeneous systems, we perform a complete stability analysis and derive explicit expressions for all integral curves. (The extremal controls of a large class of optimal control problems on SE(1,1) are linearly related to these integral curves.) Lastly, we discuss the Riemannian and sub-Riemannian problems. The (left-invariant) Riemannian and sub-Riemannian structures on SE(1,1) are classified, up to isometric group automorphisms and scaling. Explicit expressions for the geodesics of the normalised structures are found.
- Full Text:
- Date Issued: 2014
- Authors: Barrett, Dennis Ian
- Date: 2014
- Subjects: Automorphisms , Symmetry (Mathematics) , Lyapunov stability , Geometry, Riemannian , Geometry, Affine , Elliptic functions
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/64805 , vital:28605
- Description: In this thesis we consider invariant control systems and Hamilton-Poisson systems on the three dimensional semi-Euclidean group SE(1,1). We first classify the left-invariant control affine systems (under detached feedback equivalence). We provide a complete list of normal forms, as well as classifying conditions. As a corollary to this classification, we derive controllability criteria for control affine systems on SE(1,1). Secondly, we consider quadratic Hamilton-Poisson systems on the (minus) Lie-Poisson space se(1,1)*. These systems are classified up to an affine isomorphism. Six normal forms are identified for the homogeneous case, whereas sixteen representatives (including several infinite families) are obtained for the inhomogeneous systems. Thereafter we consider the stability and integration of the normal forms obtained. For all homogeneous systems, and a subclass of inhomogeneous systems, we perform a complete stability analysis and derive explicit expressions for all integral curves. (The extremal controls of a large class of optimal control problems on SE(1,1) are linearly related to these integral curves.) Lastly, we discuss the Riemannian and sub-Riemannian problems. The (left-invariant) Riemannian and sub-Riemannian structures on SE(1,1) are classified, up to isometric group automorphisms and scaling. Explicit expressions for the geodesics of the normalised structures are found.
- Full Text:
- Date Issued: 2014
The framing of China in Nigeria : an analysis of the coverage of China's involvement in Nigeria by Thisday newspaper
- Authors: Umejei, Emeka Lucky
- Date: 2014
- Subjects: Thisday (Nigeria) , Nigeria -- Foreign economic relations -- China , China -- Foreign economic relations -- Nigeria , Journalism -- Nigeria , Journalism -- Political aspects -- Nigeria , Nigerian newspapers -- Objectivity , Social responsibility of business -- Nigeria
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3525 , http://hdl.handle.net/10962/d1012974
- Description: This study identified the media frames that dominate Thisday newspaper's coverage of China's engagement with Nigeria and relate these frames to frame sponsors, who articulate and contest these framings. Frame analysis is applied to a sample of 40 news, feature and opinion articles between the sample period of 1 November 2011 and 31 December 2012. The study analysed media content from Thisday newspapers, drawing on the four dimensions of frames identified by Entman: define problems, diagnose causes, evaluate causal agents and their effects, and recommend treatment (Entman 1993). Using an inductive approach to frame analysis, the study identified two overarching mega frames, contested among the ruling elites who sponsor their views on China in the media, which define China's engagement with Nigeria; partner/role model and predator. The two mega frames mirror the broad characterisation prevalent in the academic literature on China in Africa. The primary partner/role model mega frame constructs China's engagement with Nigeria as a mutually beneficial economic partnership while on the other hand the predator mega frame constructs it as unequal and exploitative. The study identified the activities of frame sponsors who are articulating and promoting their views on China's engagement with Nigeria in the media as primarily responsible for these framings. The study also identified the activities of frame sponsors (ruling and economic elites) was key to the exclusion of ordinary peoples' voices, civic organisations, trade unions and human rights organisation in the text. However, the study also attributes the exclusion of ordinary voices, human rights, democracy and civic engagements in the text to the weakness of Thisday journalism in mediating the framings of China being promoted and articulated by elite frame sponsors. This is, however, symptomatic of the fault lines of journalism practice in Nigeria.
- Full Text:
- Date Issued: 2014
- Authors: Umejei, Emeka Lucky
- Date: 2014
- Subjects: Thisday (Nigeria) , Nigeria -- Foreign economic relations -- China , China -- Foreign economic relations -- Nigeria , Journalism -- Nigeria , Journalism -- Political aspects -- Nigeria , Nigerian newspapers -- Objectivity , Social responsibility of business -- Nigeria
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3525 , http://hdl.handle.net/10962/d1012974
- Description: This study identified the media frames that dominate Thisday newspaper's coverage of China's engagement with Nigeria and relate these frames to frame sponsors, who articulate and contest these framings. Frame analysis is applied to a sample of 40 news, feature and opinion articles between the sample period of 1 November 2011 and 31 December 2012. The study analysed media content from Thisday newspapers, drawing on the four dimensions of frames identified by Entman: define problems, diagnose causes, evaluate causal agents and their effects, and recommend treatment (Entman 1993). Using an inductive approach to frame analysis, the study identified two overarching mega frames, contested among the ruling elites who sponsor their views on China in the media, which define China's engagement with Nigeria; partner/role model and predator. The two mega frames mirror the broad characterisation prevalent in the academic literature on China in Africa. The primary partner/role model mega frame constructs China's engagement with Nigeria as a mutually beneficial economic partnership while on the other hand the predator mega frame constructs it as unequal and exploitative. The study identified the activities of frame sponsors who are articulating and promoting their views on China's engagement with Nigeria in the media as primarily responsible for these framings. The study also identified the activities of frame sponsors (ruling and economic elites) was key to the exclusion of ordinary peoples' voices, civic organisations, trade unions and human rights organisation in the text. However, the study also attributes the exclusion of ordinary voices, human rights, democracy and civic engagements in the text to the weakness of Thisday journalism in mediating the framings of China being promoted and articulated by elite frame sponsors. This is, however, symptomatic of the fault lines of journalism practice in Nigeria.
- Full Text:
- Date Issued: 2014
Can expansive (social) learning processes strengthen organisational learning for improved wetland management in a plantation forestry company, and if so how? : a case study of Mondi
- Authors: Lindley, David Stewart
- Date: 2014
- Subjects: Mondi Group , Wetland management -- South Africa , Wetland conservation -- Study and teaching (Continuing education) , Employees -- Training of -- South Africa , Action theory , Critical realism , Social learning , Environmental education -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2003 , http://hdl.handle.net/10962/d1015661
- Description: Mondi is an international packaging and paper company that manages over 300 000 ha of land in South Africa. After over a decade of working with Mondi to improve its wetland management, wetland sustainability practices were still not integrated into the broader forestry operations, despite some significant cases of successful wetland rehabilitation. An interventionist research project was therefore conducted to explore the factors inhibiting improved wetland management, and determine if and how expansive social learning processes could strengthen organisational learning and development to overcome these factors. In doing so, the research has investigated how informal adult learning supports organisational change to strengthen wetland and environmental sustainability practices, within a corporate plantation forestry context. How individual and/or group-based learning interactions translate to the collective, at the level of organisational change was a key issue probed in this study. The following three research questions were used to guide the research: 1. What tensions and contradictions exist in wetland management in a plantation forestry company? 2. Can expansive learning begin to address the tensions and contradictions that exist in wetland management in a plantation forestry company, for improved sustainability practices? 3. Can expansive social learning strengthen organisational learning and development, enabling Mondi to improve its wetland sustainability practices, and if so how does it do this? Cultural historical activity theory (CHAT) and the theory of expansive learning provided an epistemological framework for the research. The philosophy of critical realism gave ontological depth to the research, and contributed to a deeper understanding of CHAT and expansive learning. Critical realism was therefore used as a philosophy to underlabour the theoretical framework of the research. However CHAT and expansive learning could not provide the depth of detail required to explain how the expansive learning, organisational social change, and boundary crossings that are necessary for assembling the collective were taking place. Realist social theory (developed out of critical realism by Margaret Archer as an ontologically located theory of how and why social change occurs, or does not) supported the research to do this. The morphogenetic framework was used as a methodology for applying realist social theory. The expansive learning cycle was used as a methodology for applying CHAT and the theory of expansive learning; guiding the development of new knowledge creation required by Mondi staff to identify contradictions and associated tensions inhibiting wetland management, understand their root causes, and develop solutions. Through the expansive learning process, the tensions and contradictions become generative as a tool supporting expansive social learning, rather than as a means to an end where universal consensus was reached on how to circumvent the contradictions. The research was conducted in five phases: • Phase 1: Contextual profiling to identify and describe three activity systems in Mondi responsible for wetland management: 1) siviculture foresters; 2) environmental specialists; 3) community engagement facilitators. The data was generated and analysed through through document analysis, 17 interviews, 2nd generation CHAT analysis, and Critical Realist generative mechanism analysis; • Phase 2: Analysis and identification of tensions and contradictions through a first interventionist workshop. Modelling new solutions to deal with contractions, and examining and testing new models in and after the second interventionist workshop; • Phase 3: Implementing new models as wetland management projects and involved project implementation. This included boundary crossing practices of staff in the three activity systems, reflection and re-view in a further five progress review/interventionist workshops, and a management meeting and seminar; • Phase 4: Reflecting on the expansive learning process, results, and consolidation of changed practices, through nine reflective interviews and field observations; • Phase 5: Morphogenic/stasis analysis of the organisational change and development catalysed via the expansive social learning process (or not). The research found that expansive social learning processes supported organisational learning and development for improved wetland management by: 1) strengthening the scope, depth, and sophistication of participant understanding; 2) expanding the ways staff interact and collaboratively work together; 3) democratising decision making; 4) improving social relations between staff, reducing power differentials, and creating stronger relationships; 5) enhancing participant reflexivity through deeper understanding of social structures and cultural systems, and changing them to support improved wetland and environmental practice of staff, and developing the organisational structures and processes to strengthen organisational learning and development; and 6) using the contradictions identified as generative mechanisms to stimulate and catalyse organisational learning and development for changed wetland/environmental management.
- Full Text:
- Date Issued: 2014
- Authors: Lindley, David Stewart
- Date: 2014
- Subjects: Mondi Group , Wetland management -- South Africa , Wetland conservation -- Study and teaching (Continuing education) , Employees -- Training of -- South Africa , Action theory , Critical realism , Social learning , Environmental education -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2003 , http://hdl.handle.net/10962/d1015661
- Description: Mondi is an international packaging and paper company that manages over 300 000 ha of land in South Africa. After over a decade of working with Mondi to improve its wetland management, wetland sustainability practices were still not integrated into the broader forestry operations, despite some significant cases of successful wetland rehabilitation. An interventionist research project was therefore conducted to explore the factors inhibiting improved wetland management, and determine if and how expansive social learning processes could strengthen organisational learning and development to overcome these factors. In doing so, the research has investigated how informal adult learning supports organisational change to strengthen wetland and environmental sustainability practices, within a corporate plantation forestry context. How individual and/or group-based learning interactions translate to the collective, at the level of organisational change was a key issue probed in this study. The following three research questions were used to guide the research: 1. What tensions and contradictions exist in wetland management in a plantation forestry company? 2. Can expansive learning begin to address the tensions and contradictions that exist in wetland management in a plantation forestry company, for improved sustainability practices? 3. Can expansive social learning strengthen organisational learning and development, enabling Mondi to improve its wetland sustainability practices, and if so how does it do this? Cultural historical activity theory (CHAT) and the theory of expansive learning provided an epistemological framework for the research. The philosophy of critical realism gave ontological depth to the research, and contributed to a deeper understanding of CHAT and expansive learning. Critical realism was therefore used as a philosophy to underlabour the theoretical framework of the research. However CHAT and expansive learning could not provide the depth of detail required to explain how the expansive learning, organisational social change, and boundary crossings that are necessary for assembling the collective were taking place. Realist social theory (developed out of critical realism by Margaret Archer as an ontologically located theory of how and why social change occurs, or does not) supported the research to do this. The morphogenetic framework was used as a methodology for applying realist social theory. The expansive learning cycle was used as a methodology for applying CHAT and the theory of expansive learning; guiding the development of new knowledge creation required by Mondi staff to identify contradictions and associated tensions inhibiting wetland management, understand their root causes, and develop solutions. Through the expansive learning process, the tensions and contradictions become generative as a tool supporting expansive social learning, rather than as a means to an end where universal consensus was reached on how to circumvent the contradictions. The research was conducted in five phases: • Phase 1: Contextual profiling to identify and describe three activity systems in Mondi responsible for wetland management: 1) siviculture foresters; 2) environmental specialists; 3) community engagement facilitators. The data was generated and analysed through through document analysis, 17 interviews, 2nd generation CHAT analysis, and Critical Realist generative mechanism analysis; • Phase 2: Analysis and identification of tensions and contradictions through a first interventionist workshop. Modelling new solutions to deal with contractions, and examining and testing new models in and after the second interventionist workshop; • Phase 3: Implementing new models as wetland management projects and involved project implementation. This included boundary crossing practices of staff in the three activity systems, reflection and re-view in a further five progress review/interventionist workshops, and a management meeting and seminar; • Phase 4: Reflecting on the expansive learning process, results, and consolidation of changed practices, through nine reflective interviews and field observations; • Phase 5: Morphogenic/stasis analysis of the organisational change and development catalysed via the expansive social learning process (or not). The research found that expansive social learning processes supported organisational learning and development for improved wetland management by: 1) strengthening the scope, depth, and sophistication of participant understanding; 2) expanding the ways staff interact and collaboratively work together; 3) democratising decision making; 4) improving social relations between staff, reducing power differentials, and creating stronger relationships; 5) enhancing participant reflexivity through deeper understanding of social structures and cultural systems, and changing them to support improved wetland and environmental practice of staff, and developing the organisational structures and processes to strengthen organisational learning and development; and 6) using the contradictions identified as generative mechanisms to stimulate and catalyse organisational learning and development for changed wetland/environmental management.
- Full Text:
- Date Issued: 2014
Establishing a formulation design space for a generic clobetasol 17- propionate cream using the principles of quality by design
- Fauzee, Ayeshah Fateemah Beebee
- Authors: Fauzee, Ayeshah Fateemah Beebee
- Date: 2014
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:20983 , http://hdl.handle.net/10962/5868
- Description: The pharmaceutical industry is global, is highly regulated and is able to achieve reasonable product quality but at high cost with maximum effort. Numerous challenges face the pharmaceutical industry and include a shrinking research pipeline, less innovation, outsourcing, investments, increasing research and development costs, long approval times, growth of the generic industry, failure to understand or analyze manufacturing failure and wastage as high at fifty percent for some pharmaceutical products. An efficient and flexible pharmaceutical sector should be able to consistently produce high quality pharmaceutical products at a reduced cost with minimal waste. As a result, Food and Drug Administration (FDA) and other agencies such as the International Conference on Harmonization (ICH) have embraced a “Quality by Design” (QbD) paradigm and this has become the “desired state” so as to shift manufacturing from being empirical to a science, engineering, and risk based approach. QbD is a systematic approach for the development of high quality pharmaceutical dosage forms that begins with predefined objectives based on the premise that quality must be built into and not tested into a product. QbD together with the establishment of a design space for dosage forms is a fairly new concept and there is limited published data on QbD concepts that report the entire process of identifying Critical Quality Attributes (CQA), design of a formulation and manufacturing process to meet product CQA, understanding the impact of material attributes and process parameters on product CQA, identification and controlling sources of variability in materials and processes that affect the CQA of a product and finally establishing, evaluating and testing a design space using both in vitro and in vivo approaches to assure that a product of consistent quality can always be produced. The objective of these studies was to implement a QbD approach to establish a design space for the development and manufacture of a safe, effective, stable generic formulation containing 0.05% w/w clobetasol 17-propionate (CP) that had similar in vitro and in vivo characteristics to an innovator product, Dermovate® (Sekpharma® Pty Ltd, Sandton, Gauteng, RSA). Such a product would pose a minimal risk of failure when treating severe skin disorders such as seborrhoeic dermatitis, extreme photodermatitis and/or severe psoriasis in HIV/AIDS patients in Southern Africa.
- Full Text:
- Date Issued: 2014
- Authors: Fauzee, Ayeshah Fateemah Beebee
- Date: 2014
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:20983 , http://hdl.handle.net/10962/5868
- Description: The pharmaceutical industry is global, is highly regulated and is able to achieve reasonable product quality but at high cost with maximum effort. Numerous challenges face the pharmaceutical industry and include a shrinking research pipeline, less innovation, outsourcing, investments, increasing research and development costs, long approval times, growth of the generic industry, failure to understand or analyze manufacturing failure and wastage as high at fifty percent for some pharmaceutical products. An efficient and flexible pharmaceutical sector should be able to consistently produce high quality pharmaceutical products at a reduced cost with minimal waste. As a result, Food and Drug Administration (FDA) and other agencies such as the International Conference on Harmonization (ICH) have embraced a “Quality by Design” (QbD) paradigm and this has become the “desired state” so as to shift manufacturing from being empirical to a science, engineering, and risk based approach. QbD is a systematic approach for the development of high quality pharmaceutical dosage forms that begins with predefined objectives based on the premise that quality must be built into and not tested into a product. QbD together with the establishment of a design space for dosage forms is a fairly new concept and there is limited published data on QbD concepts that report the entire process of identifying Critical Quality Attributes (CQA), design of a formulation and manufacturing process to meet product CQA, understanding the impact of material attributes and process parameters on product CQA, identification and controlling sources of variability in materials and processes that affect the CQA of a product and finally establishing, evaluating and testing a design space using both in vitro and in vivo approaches to assure that a product of consistent quality can always be produced. The objective of these studies was to implement a QbD approach to establish a design space for the development and manufacture of a safe, effective, stable generic formulation containing 0.05% w/w clobetasol 17-propionate (CP) that had similar in vitro and in vivo characteristics to an innovator product, Dermovate® (Sekpharma® Pty Ltd, Sandton, Gauteng, RSA). Such a product would pose a minimal risk of failure when treating severe skin disorders such as seborrhoeic dermatitis, extreme photodermatitis and/or severe psoriasis in HIV/AIDS patients in Southern Africa.
- Full Text:
- Date Issued: 2014
The natural history of the humpback dolphin, Sousa chinensis, in KwaZulu-Natal, South Africa : age, growth and reproduction
- Authors: Nolte, Zianca
- Date: 2014
- Subjects: Sousa -- South Africa -- KwaZulu-Natal , Dolphins -- Behavior -- South Africa -- KwaZulu-Natal , Dolphins -- Age determination -- South Africa -- KwaZulu-Natal , Dolphins -- Development -- South Africa -- KwaZulu-Natal , Dolphins -- South Africa -- KwaZulu-Natal -- Reproduction , Dolphins -- Physiology -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5878 , http://hdl.handle.net/10962/d1013178
- Description: Globally, inshore cetaceans are being threatened by a number of anthropogenic activities. The Indo-Pacific humpback dolphin, Sousa chinensis, is currently listed as ‘near threatened’ by the International Union for the Conservation of Nature (IUCN). In order to be able to advise on management and conservation strategies, knowledge on the life history of the species is required. To date very little is known about the biology of humpback dolphins. The aim of the present study was to determine basic life history parameters, including age, growth and reproduction of humpback dolphins incidentally caught in shark nets. Age was estimated by counting the growth layer groups (GLGs) in the dentine and cementum of sectioned and stained teeth. Both a Von Bertalanffy and a Gompertz growth curve fitted well to the data, but for comparison with previous studies on Sousa, the Gompertz growth function was adopted to describe the relationship between length and age for KwaZulu-Natal populations. Length at birth was estimated between 104.33 and 111.57 cm for males and females, respectively. Asymptotic length was reached at 266.48 cm and 239.29 cm for males and females, respectively. This corresponds to the attainment of physical maturity at 24 GLGs in males and 16 GLGs in females. Asymptotic mass for males could not be determined, while for females it occurred around 160 kg. The maximum age estimates and recorded lengths were 24 GLGs and 279 cm for males and 17.7 GLGs and 249 cm for females. Differences in length-at-age and mass-at-age for S. chinensis suggest sexual dimorphism. The attainment of sexual maturity in males occurred between 9 and 10 GLGs, corresponding to 230 cm total body length and 140 kg. The maximum combined testis mass of mature males comprised 0.42% of total body mass, and a roving male mating system was proposed. In females, sexual maturity occurred around 7.6 GLG, between 220 and 222 cm and 104 - 140 kg. The ovulation rate is estimated at 0.2 ovulations per annum, suggesting a calving interval of five years. It is evident from the results obtained in the present study that geographical differences exist in the life history parameters of S. chinensis. As a result, regional conservation and management strategies are imperative. Results from this study can therefore assist in assessing the status of existing population structures in the KwaZulu-Natal coastal waters, and the implementation of regional mitigation strategies to ensure the continued survival of humpback dolphins in the region.
- Full Text:
- Date Issued: 2014
- Authors: Nolte, Zianca
- Date: 2014
- Subjects: Sousa -- South Africa -- KwaZulu-Natal , Dolphins -- Behavior -- South Africa -- KwaZulu-Natal , Dolphins -- Age determination -- South Africa -- KwaZulu-Natal , Dolphins -- Development -- South Africa -- KwaZulu-Natal , Dolphins -- South Africa -- KwaZulu-Natal -- Reproduction , Dolphins -- Physiology -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5878 , http://hdl.handle.net/10962/d1013178
- Description: Globally, inshore cetaceans are being threatened by a number of anthropogenic activities. The Indo-Pacific humpback dolphin, Sousa chinensis, is currently listed as ‘near threatened’ by the International Union for the Conservation of Nature (IUCN). In order to be able to advise on management and conservation strategies, knowledge on the life history of the species is required. To date very little is known about the biology of humpback dolphins. The aim of the present study was to determine basic life history parameters, including age, growth and reproduction of humpback dolphins incidentally caught in shark nets. Age was estimated by counting the growth layer groups (GLGs) in the dentine and cementum of sectioned and stained teeth. Both a Von Bertalanffy and a Gompertz growth curve fitted well to the data, but for comparison with previous studies on Sousa, the Gompertz growth function was adopted to describe the relationship between length and age for KwaZulu-Natal populations. Length at birth was estimated between 104.33 and 111.57 cm for males and females, respectively. Asymptotic length was reached at 266.48 cm and 239.29 cm for males and females, respectively. This corresponds to the attainment of physical maturity at 24 GLGs in males and 16 GLGs in females. Asymptotic mass for males could not be determined, while for females it occurred around 160 kg. The maximum age estimates and recorded lengths were 24 GLGs and 279 cm for males and 17.7 GLGs and 249 cm for females. Differences in length-at-age and mass-at-age for S. chinensis suggest sexual dimorphism. The attainment of sexual maturity in males occurred between 9 and 10 GLGs, corresponding to 230 cm total body length and 140 kg. The maximum combined testis mass of mature males comprised 0.42% of total body mass, and a roving male mating system was proposed. In females, sexual maturity occurred around 7.6 GLG, between 220 and 222 cm and 104 - 140 kg. The ovulation rate is estimated at 0.2 ovulations per annum, suggesting a calving interval of five years. It is evident from the results obtained in the present study that geographical differences exist in the life history parameters of S. chinensis. As a result, regional conservation and management strategies are imperative. Results from this study can therefore assist in assessing the status of existing population structures in the KwaZulu-Natal coastal waters, and the implementation of regional mitigation strategies to ensure the continued survival of humpback dolphins in the region.
- Full Text:
- Date Issued: 2014
Learning pathways for improving rehabilitation practices in the mining industry : two cases of coal mining and borrow pits
- Authors: Mphinyane, Andani
- Date: 2014
- Subjects: Coal mines and mining -- Environmental aspects -- South Africa , Environmental education -- South Africa , Sustainable development -- South Africa , Coal miners -- Education (Continuing education) -- South Africa , Borrow pits -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1989 , http://hdl.handle.net/10962/d1013271
- Description: This research investigates cases of learning pathways for improving rehabilitation practices for key occupations in the mining industry. The study is set up as a partnership research programme between Rhodes University Environmental Learning Research Centre in South Africa, and the South African Qualification Authority, focussing on workplace learning and sustainability practices. This research programme seeks to understand the implications of the move to a knowledge society, with its emphasis on knowledge building over time, particularly in and for the environmental sector. The research was conducted as a qualitative case study that made use of semi-structured interviews, document analysis, visual photographs and observations as instruments of data gathering. Participants were sampled from two case studies, one in Limpopo province and the other one in Mpumalanga Province, who are directly involved in rehabilitation practices and related education and training programmes. The study makes use of career stories from the key occupations to provide insight into workplace learning pathways to inform education and training in the mining industry. A series of analytical statements captures some of the main findings on early education histories, career choices, learning pathway decisions and experiences related to sustainable practices and some complexities related to learning pathways. Environment and sustainability education is a cross-cutting issue in the NQF; and it pertains to the mining sector, especially to rehabilitation practices, which form the focus of this study as little is known about learning pathways associated with these sustainability practices. Insights from the study should enable the sector to enhance rehabilitation training for key occupations and at the same time encourage lifelong learning contributing towards sustainable development.
- Full Text:
- Date Issued: 2014
- Authors: Mphinyane, Andani
- Date: 2014
- Subjects: Coal mines and mining -- Environmental aspects -- South Africa , Environmental education -- South Africa , Sustainable development -- South Africa , Coal miners -- Education (Continuing education) -- South Africa , Borrow pits -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1989 , http://hdl.handle.net/10962/d1013271
- Description: This research investigates cases of learning pathways for improving rehabilitation practices for key occupations in the mining industry. The study is set up as a partnership research programme between Rhodes University Environmental Learning Research Centre in South Africa, and the South African Qualification Authority, focussing on workplace learning and sustainability practices. This research programme seeks to understand the implications of the move to a knowledge society, with its emphasis on knowledge building over time, particularly in and for the environmental sector. The research was conducted as a qualitative case study that made use of semi-structured interviews, document analysis, visual photographs and observations as instruments of data gathering. Participants were sampled from two case studies, one in Limpopo province and the other one in Mpumalanga Province, who are directly involved in rehabilitation practices and related education and training programmes. The study makes use of career stories from the key occupations to provide insight into workplace learning pathways to inform education and training in the mining industry. A series of analytical statements captures some of the main findings on early education histories, career choices, learning pathway decisions and experiences related to sustainable practices and some complexities related to learning pathways. Environment and sustainability education is a cross-cutting issue in the NQF; and it pertains to the mining sector, especially to rehabilitation practices, which form the focus of this study as little is known about learning pathways associated with these sustainability practices. Insights from the study should enable the sector to enhance rehabilitation training for key occupations and at the same time encourage lifelong learning contributing towards sustainable development.
- Full Text:
- Date Issued: 2014
Considerations for implementating market based mechanisms in combating climate change in South Africa
- Authors: Marais, Frans
- Date: 2014
- Subjects: Climatic changes -- Economic aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1093 , http://hdl.handle.net/10962/d1012952
- Description: Since the first period of the Kyoto Protocol, there has been a growing concern that the burden of reducing greenhouse gas emissions should not only be borne by developed countries, but developing countries as well. South Africa, as the 18th highest emitter of greenhouse gases in the world and highest in Africa, has a significant responsibility to reduce its emissions levels. The South African government is currently in the process of implementing a carbon tax for its short term response to climate change and considering the implementation of a carbon market as a medium to long term response to climate change. Both of these market based mechanisms are widely deemed effective in the mitigation of greenhouse gas emissions by economists, however are also known to have negative social and economic implications upon an economy. This study identifies these implications and attempts to provide considerations on how to alleviate the implications through the most appropriate process of revenue recycling. The negative effects of Implementing a carbon tax or carbon market could be severe as and not limited to: a significant decline in GDP, a reduction in the standard of living for certain households, a fall in a country's exports and even an increase in poverty. South Africa's environmental and development policies place a strict precedence on the protection of the poor and the prevention of economic hardship induced by such policies. This places significant importance on the prevention of these externalities from occurring. A primary means of doing so is through the process of revenue recycling, however, certain channels of revenue recycling are by no means helpful, hence the most appropriate channel needs to be identified. The study carried out a multiple case study analysis on Ireland, Mexico, New Zealand and Norway, to determine what effects a carbon tax had on their economies and how these effects were mitigated through carbon tax revenue recycling. An additional analysis of the EU ETS was carried out to determine how the EU ETS was implemented and the controversies and concerns that arose during its implementation. The findings of this analysis were then compared to a number of South African economist’s case studies, and the most appropriate method of revenue recycling identified and possible solutions to the EU ETS controversies found. The study concludes that a food subsidy has the potential to provide positive effects on welfare employment and GDP; therefore could be considered to be the most appropriate method of revenue recycling. However, these effects are limited to be experienced only at low levels of a carbon tax, hence, short term in nature. The study therefore provides a further consideration that the use of multiple channels for revenue recycling needs to be explored that could provide stable longer term effects. In addition, in the implementation of a carbon market, the study concludes that government should consider using an auction approach in the initial allocation phase of an ETS and the use of a centralized registry for monitoring and controlling of information and transactions.
- Full Text:
- Date Issued: 2014
- Authors: Marais, Frans
- Date: 2014
- Subjects: Climatic changes -- Economic aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1093 , http://hdl.handle.net/10962/d1012952
- Description: Since the first period of the Kyoto Protocol, there has been a growing concern that the burden of reducing greenhouse gas emissions should not only be borne by developed countries, but developing countries as well. South Africa, as the 18th highest emitter of greenhouse gases in the world and highest in Africa, has a significant responsibility to reduce its emissions levels. The South African government is currently in the process of implementing a carbon tax for its short term response to climate change and considering the implementation of a carbon market as a medium to long term response to climate change. Both of these market based mechanisms are widely deemed effective in the mitigation of greenhouse gas emissions by economists, however are also known to have negative social and economic implications upon an economy. This study identifies these implications and attempts to provide considerations on how to alleviate the implications through the most appropriate process of revenue recycling. The negative effects of Implementing a carbon tax or carbon market could be severe as and not limited to: a significant decline in GDP, a reduction in the standard of living for certain households, a fall in a country's exports and even an increase in poverty. South Africa's environmental and development policies place a strict precedence on the protection of the poor and the prevention of economic hardship induced by such policies. This places significant importance on the prevention of these externalities from occurring. A primary means of doing so is through the process of revenue recycling, however, certain channels of revenue recycling are by no means helpful, hence the most appropriate channel needs to be identified. The study carried out a multiple case study analysis on Ireland, Mexico, New Zealand and Norway, to determine what effects a carbon tax had on their economies and how these effects were mitigated through carbon tax revenue recycling. An additional analysis of the EU ETS was carried out to determine how the EU ETS was implemented and the controversies and concerns that arose during its implementation. The findings of this analysis were then compared to a number of South African economist’s case studies, and the most appropriate method of revenue recycling identified and possible solutions to the EU ETS controversies found. The study concludes that a food subsidy has the potential to provide positive effects on welfare employment and GDP; therefore could be considered to be the most appropriate method of revenue recycling. However, these effects are limited to be experienced only at low levels of a carbon tax, hence, short term in nature. The study therefore provides a further consideration that the use of multiple channels for revenue recycling needs to be explored that could provide stable longer term effects. In addition, in the implementation of a carbon market, the study concludes that government should consider using an auction approach in the initial allocation phase of an ETS and the use of a centralized registry for monitoring and controlling of information and transactions.
- Full Text:
- Date Issued: 2014