Links between valley confinement, landforms and vegetation distribution in a semi-arid valley floor environment, Baviaanskloof, South Africa
- Authors: Smith-Adao, Lindie B
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/953 , vital:20006
- Description: Although a large body of international literature has advanced our understanding of river systems, a considerable amount of gaps exit in the knowledge of dryland systems. River systems reflect complex interactions between biophysical processes and patterns. Understanding how processes generate observed patterns and, in turn, how patterns influence processes is crucial to understanding river structure and function. It requires an interdisciplinary approach in both research and resulting applications. The aim of this thesis was to examine the relationship between valley confinement, fluvial style, valley floor morphology and vegetation in the semi-arid environment of the Baviaanskloof river catchment, South Africa. This interdisciplinary investigation used a mixed method approach that involved desktop analyses and field surveys to understand dynamics at multiple scales, from the whole catchment to local (site, reach and quadrat or sample plot) scales. The desktop analyses included historical rainfall patterns and climate extremes, aerial photograph time-series and remote sensing greenness indices, and the field surveys focussed on cross-valley landform profiles, groundwater depth levels, sediment size distribution and soil chemistry, and vegetation distribution patterns. Based on the integrative assessment of these techniques a framework was developed of the links between valley confinement, surface-groundwater interaction, hydrogeomorphic processes and landforms, vegetation and human activities. Study findings highlighted the fact that very strong links exist between these factors. In this semi-arid area water availability (groundwater and streamflow conditions) was the primary control on valley floor vegetation composition and patterning. One group of species was associated with the wetted channel reaches at confined and semi-confined valley settings, while the other was associated with dry bed channel reaches at unconfined valley settings. The analyses also indicated that the environmental variables which best explained the variation in vegetation at the differing geomorphic landforms were related to landform position (elevation and distance), sediment size (fine and coarse sand) and available phosphorus (i.e. human impacts). The different plants, in turn, also affected landforms through their influence on sediment erosion, transport and deposition. Specially, they build, maintained or modified geomorphic landforms at confined, semi-confined and unconfined valley settings across the valley floor. Valley confinement was a primary control influencing hydrogeomorphic processes and their associated landforms. Alluvial fans and terraces acted as critical additional controls at especially the unconfined valley setting. Implications for restoration efforts in the catchment included strategies that took cognisance of: active revegetation on floodplain surfaces related to former agricultural fields; channel switching associated with the highly dynamic and unpredictable nature of geomorphic features; unstable multi-thread braided channels in the unconfined valley setting; and the selection of representative monitoring sites.
- Full Text:
- Date Issued: 2016
- Authors: Smith-Adao, Lindie B
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/953 , vital:20006
- Description: Although a large body of international literature has advanced our understanding of river systems, a considerable amount of gaps exit in the knowledge of dryland systems. River systems reflect complex interactions between biophysical processes and patterns. Understanding how processes generate observed patterns and, in turn, how patterns influence processes is crucial to understanding river structure and function. It requires an interdisciplinary approach in both research and resulting applications. The aim of this thesis was to examine the relationship between valley confinement, fluvial style, valley floor morphology and vegetation in the semi-arid environment of the Baviaanskloof river catchment, South Africa. This interdisciplinary investigation used a mixed method approach that involved desktop analyses and field surveys to understand dynamics at multiple scales, from the whole catchment to local (site, reach and quadrat or sample plot) scales. The desktop analyses included historical rainfall patterns and climate extremes, aerial photograph time-series and remote sensing greenness indices, and the field surveys focussed on cross-valley landform profiles, groundwater depth levels, sediment size distribution and soil chemistry, and vegetation distribution patterns. Based on the integrative assessment of these techniques a framework was developed of the links between valley confinement, surface-groundwater interaction, hydrogeomorphic processes and landforms, vegetation and human activities. Study findings highlighted the fact that very strong links exist between these factors. In this semi-arid area water availability (groundwater and streamflow conditions) was the primary control on valley floor vegetation composition and patterning. One group of species was associated with the wetted channel reaches at confined and semi-confined valley settings, while the other was associated with dry bed channel reaches at unconfined valley settings. The analyses also indicated that the environmental variables which best explained the variation in vegetation at the differing geomorphic landforms were related to landform position (elevation and distance), sediment size (fine and coarse sand) and available phosphorus (i.e. human impacts). The different plants, in turn, also affected landforms through their influence on sediment erosion, transport and deposition. Specially, they build, maintained or modified geomorphic landforms at confined, semi-confined and unconfined valley settings across the valley floor. Valley confinement was a primary control influencing hydrogeomorphic processes and their associated landforms. Alluvial fans and terraces acted as critical additional controls at especially the unconfined valley setting. Implications for restoration efforts in the catchment included strategies that took cognisance of: active revegetation on floodplain surfaces related to former agricultural fields; channel switching associated with the highly dynamic and unpredictable nature of geomorphic features; unstable multi-thread braided channels in the unconfined valley setting; and the selection of representative monitoring sites.
- Full Text:
- Date Issued: 2016
Observational cosmology with imperfect data
- Authors: Bester, Hertzog Landman
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/463 , vital:19961
- Description: We develop a formalism suitable to infer the background geometry of a general spherically symmetric dust universe directly from data on the past lightcone. This direct observational approach makes minimal assumptions about inaccessible parts of the Universe. The non-parametric and Bayesian framework we propose provides a very direct way to test one of the most fundamental underlying assumptions of concordance cosmology viz. the Copernican principle. We present the Copernicus algorithm for this purpose. By applying the algorithm to currently available data, we demonstrate that it is not yet possible to confirm or refute the validity of the Copernican principle within the proposed framework. This is followed by an investigation which aims to determine which future data will best be able to test the Copernican principle. Our results on simulated data suggest that, besides the need to improve the current data, it will be important to identify additional model independent observables for this purpose. The main difficulty with current data is their inability to constrain the value of the cosmological constant. We show how redshift drift data could be used to infer its value with minimal assumptions about the nature of the early Universe. We also discuss some alternative applications of the algorithm.
- Full Text:
- Date Issued: 2016
- Authors: Bester, Hertzog Landman
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/463 , vital:19961
- Description: We develop a formalism suitable to infer the background geometry of a general spherically symmetric dust universe directly from data on the past lightcone. This direct observational approach makes minimal assumptions about inaccessible parts of the Universe. The non-parametric and Bayesian framework we propose provides a very direct way to test one of the most fundamental underlying assumptions of concordance cosmology viz. the Copernican principle. We present the Copernicus algorithm for this purpose. By applying the algorithm to currently available data, we demonstrate that it is not yet possible to confirm or refute the validity of the Copernican principle within the proposed framework. This is followed by an investigation which aims to determine which future data will best be able to test the Copernican principle. Our results on simulated data suggest that, besides the need to improve the current data, it will be important to identify additional model independent observables for this purpose. The main difficulty with current data is their inability to constrain the value of the cosmological constant. We show how redshift drift data could be used to infer its value with minimal assumptions about the nature of the early Universe. We also discuss some alternative applications of the algorithm.
- Full Text:
- Date Issued: 2016
Cosmological structure formation using spectral methods
- Authors: Funcke, Michelle
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2969 , vital:20348
- Description: Numerical simulations are becoming an increasingly important tool for understanding the growth and development of structure in the universe. Common practice is to discretize the space-time using physical variables. The discreteness is embodied by considering the dynamical variables as fields on a fixed spatial and time resolution, or by constructing the matter fields by a large number of particles which interact gravitationally (N-body methods). Recognizing that the physical quantities of interest are related to the spectrum of perturbations, we propose an alternate discretization in the frequency domain, using standard spectral methods. This approach is further aided by periodic boundary conditions which allows a straightforward decomposition of variables in a Fourier basis. Fixed resources require a high-frequency cut-off which lead to aliasing effects in non-linear equations, such as the ones considered here. This thesis describes the implementation of a 3D cosmological model based on Newtonian hydrodynamic equations in an expanding background. Initial data is constructed as a spectrum of perturbations, and evolved in the frequency domain using a pseudo-spectral evolution scheme and an explicit Runge-Kutta time integrator. The code is found to converge for both linear and non-linear evolutions, and the convergence rate is determined. The correct growth rates expected from analytical calculations are recovered in the linear case. In the non-linear model, we observe close correspondence with linear growth and are able to monitor the growth on features associated with the non-linearity. High-frequency aliasing effects were evident in the non-linear evolutions, leading to a study of two potential resolutions to this problem: a boxcar filter which adheres to“Orszag’s two thirds rule” and an exponential window function, the exponential filter suggested by Hou and Li [1], and a shifted version of the exponential filter suggested, which has the potential to alleviate high frequency- ripples resulting from the Gibbs’ phenomenon. We found that the filters were somewhat successful at reducing aliasing effects but that the Gibbs’ phenomenon could not be entirely removed by the choice of filters.
- Full Text:
- Date Issued: 2016
- Authors: Funcke, Michelle
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2969 , vital:20348
- Description: Numerical simulations are becoming an increasingly important tool for understanding the growth and development of structure in the universe. Common practice is to discretize the space-time using physical variables. The discreteness is embodied by considering the dynamical variables as fields on a fixed spatial and time resolution, or by constructing the matter fields by a large number of particles which interact gravitationally (N-body methods). Recognizing that the physical quantities of interest are related to the spectrum of perturbations, we propose an alternate discretization in the frequency domain, using standard spectral methods. This approach is further aided by periodic boundary conditions which allows a straightforward decomposition of variables in a Fourier basis. Fixed resources require a high-frequency cut-off which lead to aliasing effects in non-linear equations, such as the ones considered here. This thesis describes the implementation of a 3D cosmological model based on Newtonian hydrodynamic equations in an expanding background. Initial data is constructed as a spectrum of perturbations, and evolved in the frequency domain using a pseudo-spectral evolution scheme and an explicit Runge-Kutta time integrator. The code is found to converge for both linear and non-linear evolutions, and the convergence rate is determined. The correct growth rates expected from analytical calculations are recovered in the linear case. In the non-linear model, we observe close correspondence with linear growth and are able to monitor the growth on features associated with the non-linearity. High-frequency aliasing effects were evident in the non-linear evolutions, leading to a study of two potential resolutions to this problem: a boxcar filter which adheres to“Orszag’s two thirds rule” and an exponential window function, the exponential filter suggested by Hou and Li [1], and a shifted version of the exponential filter suggested, which has the potential to alleviate high frequency- ripples resulting from the Gibbs’ phenomenon. We found that the filters were somewhat successful at reducing aliasing effects but that the Gibbs’ phenomenon could not be entirely removed by the choice of filters.
- Full Text:
- Date Issued: 2016
Thermoluminescence of annealed synthetic quartz
- Atang, Elizabeth Fende Midiki
- Authors: Atang, Elizabeth Fende Midiki
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/420 , vital:19957
- Description: The kinetic and dosimetric features of the main thermoluminescent peak of synthetic quartz have been investigated in quartz ordinarily annealed at 500_C as well as quartz annealed at 500_C for 10 minutes. The main peak is found at 78 _C for the samples annealed at 500_C for 10 minutes irradiated to 10 Gy and heated at 1.0 _C/s. For the samples ordinarily annealed at 500_C the main peak is found at 106 _C after the sample has been irradiated to 30 Gy and heated at 5.0 _C/s. In these samples, the intensity of the main peak is enhanced with repetitive measurement whereas its maximum temperature is unaffected. The peak position of the main peak in the sample is independent of the irradiation dose and this, together with its fading characteristics, are consistent with first-order kinetics. For doses between 5 and 25 Gy, the dose response of the main peak of the annealed sample is superlinear. The half-life of the main TL peak of the annealed sample is about 1 h. The activation energy E of the main peak is around 0.90 eV. For a heating rate of 0.4 _C/s, its order of kinetics b derived from the whole curve method of analysis is 1.0. Following irradiation, preheating and illumination with 470 nm blue light, the main peak in the annealed sample is regenerated during heating. The resulting phototransferred peak occurs at the same temperature as the original peak and has similar kinetic and dosimetric features, with a half-life of about 1 h. For a preheat temperature of 200 _C, the intensity of the phototransferred peak in the sample increases with illumination time up to a maximum and decreases thereafter. At longer illumination times, no further decrease in the intensity of the phototransferred peak is observed. The traps associated with the 325 _C peak are the main source of the electrons responsible for the regenerated peak.
- Full Text:
- Date Issued: 2016
- Authors: Atang, Elizabeth Fende Midiki
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/420 , vital:19957
- Description: The kinetic and dosimetric features of the main thermoluminescent peak of synthetic quartz have been investigated in quartz ordinarily annealed at 500_C as well as quartz annealed at 500_C for 10 minutes. The main peak is found at 78 _C for the samples annealed at 500_C for 10 minutes irradiated to 10 Gy and heated at 1.0 _C/s. For the samples ordinarily annealed at 500_C the main peak is found at 106 _C after the sample has been irradiated to 30 Gy and heated at 5.0 _C/s. In these samples, the intensity of the main peak is enhanced with repetitive measurement whereas its maximum temperature is unaffected. The peak position of the main peak in the sample is independent of the irradiation dose and this, together with its fading characteristics, are consistent with first-order kinetics. For doses between 5 and 25 Gy, the dose response of the main peak of the annealed sample is superlinear. The half-life of the main TL peak of the annealed sample is about 1 h. The activation energy E of the main peak is around 0.90 eV. For a heating rate of 0.4 _C/s, its order of kinetics b derived from the whole curve method of analysis is 1.0. Following irradiation, preheating and illumination with 470 nm blue light, the main peak in the annealed sample is regenerated during heating. The resulting phototransferred peak occurs at the same temperature as the original peak and has similar kinetic and dosimetric features, with a half-life of about 1 h. For a preheat temperature of 200 _C, the intensity of the phototransferred peak in the sample increases with illumination time up to a maximum and decreases thereafter. At longer illumination times, no further decrease in the intensity of the phototransferred peak is observed. The traps associated with the 325 _C peak are the main source of the electrons responsible for the regenerated peak.
- Full Text:
- Date Issued: 2016
Being a black mine worker in South Africa: the case of Anglo Platinum Mine
- Authors: Maseko, Robert
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/651 , vital:19978
- Description: This thesis presents a decolonial perspective on the experience of being a black mineworker in post-apartheid South Africa with specific reference to the Platinum Belt. It seeks to understand what it means to be a black mineworker by unmasking and analysing the existence and prevalence of coloniality in contemporary South Africa despite the end of formal colonialism (i.e. apartheid). As a world-wide system, coloniality has different dimensions which all speak to and highlight continuities between the period of colonialism and the post-colonial period. These dimensions are coloniality of power, coloniality of being and coloniality of knowledge. The power structure of coloniality produces and reproduces the identity of the black mineworker in present-day South Africa as a sub-ontological being devoid of an authentic humanity such that the mineworker is depicted as incapable of rational thought and knowledge. The existential condition of the black mineworker is symptomatic of the generic experience of being a racialised subject of colour in the current global power structure predicated on the dominance and hegemony of Western-centred modernity. The black mineworker exists on the darker side of Western-centred modernity, living a life of wretchedness and continuing to suffer the colonial wound in the absence of formal colonialism and apartheid. The mineworker is disposable and dispensable and lives and works in the shadow of death. In pursuing this course of reasoning, I deploy the epistemic method of ‘shifting the geography of reason’ in order to read the experience of mineworkers in South Africa from the locus of enunciation of the oppressed subject within the scheme of a colonial power differential based on a hierarchy of humanity. This method allows me to speak with and from the perspective of the black mineworkers in the Platinum Belt as opposed to speaking for and about them. I reach the conclusion that being a platinum mineworker in post-apartheid South Africa is a racial and market determined identity of colonialised subjectivity that relegates the dominated subject (the black mineworker) to the realm of the subhuman. In setting the context for this claim, I trace the origins and development of the black mineworker in South Africa with reference to historical processes such as dispossession and proletarianisation. Empirically, the thesis is rooted in a contemporary case study of mainly Anglo Platinum Mine, which involved comprehensive fieldwork focusing on the present lived realities of platinum mineworkers. The dignity and humanity of these black mineworkers has still not returned despite twenty years of democratic rule in South Africa, such that race remains a crucial organising principle in postapartheid South Africa.
- Full Text:
- Date Issued: 2016
- Authors: Maseko, Robert
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/651 , vital:19978
- Description: This thesis presents a decolonial perspective on the experience of being a black mineworker in post-apartheid South Africa with specific reference to the Platinum Belt. It seeks to understand what it means to be a black mineworker by unmasking and analysing the existence and prevalence of coloniality in contemporary South Africa despite the end of formal colonialism (i.e. apartheid). As a world-wide system, coloniality has different dimensions which all speak to and highlight continuities between the period of colonialism and the post-colonial period. These dimensions are coloniality of power, coloniality of being and coloniality of knowledge. The power structure of coloniality produces and reproduces the identity of the black mineworker in present-day South Africa as a sub-ontological being devoid of an authentic humanity such that the mineworker is depicted as incapable of rational thought and knowledge. The existential condition of the black mineworker is symptomatic of the generic experience of being a racialised subject of colour in the current global power structure predicated on the dominance and hegemony of Western-centred modernity. The black mineworker exists on the darker side of Western-centred modernity, living a life of wretchedness and continuing to suffer the colonial wound in the absence of formal colonialism and apartheid. The mineworker is disposable and dispensable and lives and works in the shadow of death. In pursuing this course of reasoning, I deploy the epistemic method of ‘shifting the geography of reason’ in order to read the experience of mineworkers in South Africa from the locus of enunciation of the oppressed subject within the scheme of a colonial power differential based on a hierarchy of humanity. This method allows me to speak with and from the perspective of the black mineworkers in the Platinum Belt as opposed to speaking for and about them. I reach the conclusion that being a platinum mineworker in post-apartheid South Africa is a racial and market determined identity of colonialised subjectivity that relegates the dominated subject (the black mineworker) to the realm of the subhuman. In setting the context for this claim, I trace the origins and development of the black mineworker in South Africa with reference to historical processes such as dispossession and proletarianisation. Empirically, the thesis is rooted in a contemporary case study of mainly Anglo Platinum Mine, which involved comprehensive fieldwork focusing on the present lived realities of platinum mineworkers. The dignity and humanity of these black mineworkers has still not returned despite twenty years of democratic rule in South Africa, such that race remains a crucial organising principle in postapartheid South Africa.
- Full Text:
- Date Issued: 2016
Biology and ecology of Glossogobius callidus (Smith 1937) in irrigation impoundments in the Sundays River Valley of the Eastern Cape
- Authors: Mofu, Lubabalo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/1136 , vital:20023
- Description: The River Goby Glossogobius callidus (Smith, 1937) is a native abundant fish in both freshwater and estuarine habitats in the Cape Fold Ecoregion, yet little information is available on its life-history. This study aims to contribute to knowledge on the age and growth, reproductive biology and the diet and feeding habits of G. callidus in irrigation impoundments. Glossogobius callidus was sampled monthly from August 2013 till March 2015; from the irrigation ponds in the Sundays River Valley, Eastern Cape Province, South Africa. To determine sex, gonads were visually assessed under a dissecting microscope to confirm the sex based on the shape of the urogenital papillae. Fish were then dissected to confirm sex and gonads were categorised into five macroscopic stages which were histologically validated. Microscopic stages of gonadal development were discerned based on nuclear and cytoplasmic characteristics of the oocyte or sperm. Ovaries and sperms were assigned stages based on the most advanced type of oocyte present. In total 2054 fishes ranging in length from 21.1 mm to 137.2 mm TL were sampled. The sex ratio (1.1 males: 1 females) did not differ from unity (x2 = 0.027, df = 1, p = 0.87). Length at 50% maturity (Lm) was 70 mm TL females and 72 mm TL for males. Spawning season was mid-spring and mid-summer and mean ± S.D absolute fecundity was estimated at 1028.2 ± 131.7 ova/fish. Relative fecundity (number of vitellogenic oocytes per gram of eviscerated fish mass) were estimated at 50 ± 18 ova/fish gram. Otoliths from 560 fish were used for ageing. Growth zone deposition rate was validated using edge analysis. As a unimodal periodic regression model best described the temporal proportion of opaque zone deposition on the edge of otoliths over a one-year period, growth zone deposition rate was validated as annual. The oldest female fish was a 4-year old 84.4 mm TL fish and the oldest male was a 7-year old 100.5 mm TL fish. The length-at-age for the entire population of 560 G. callidus provided von Bertalanffy parameters of Lt = 92 (1 - e -0.58(t + 0.4)) mm TL for the entire population, Lt = 70 (1 - e -1.8 (t + 0.06)) mm TL for males and Lt = 65 (1 - e -1.8 (t + 0.05)) mm TL for females. Converting length at maturity to age at maturity demonstrated that G. callidus attained maturity at an age of 2-years. Growth performance described using the phi-prime index showed that G. callidus had lower growth performance compared to the invasive Neogobius melanostomus. Using age structure, natural mortality was estimated at 1.31 yr-1 using catch curve analysis. Diet of G. callidus comprised of ten taxonomic groups. Among these, aquatic invertebrates were the most diverse group but while relative contribution of the dietary components varied across all size classes and seasons, the key prey items were consistently found in all size classes. These were Diptera, Hemiptera, Trichoptera, Odonata, Cladocera, Copepoda, Hydracarina, Amphipoda, Crustacea, and Mollusca. While dietary differences were observed between the size classes and throughout the seasons, G. callidus can be regarded as a generalist feeder preying on an array of different species. Given its abundance and diet, I suggest that G. callidus contribute considerably to the invertebrate predation pressure in these artificial aquatic environments in an arid region. In summary, medium fecundity, fast growth, moderate maturity, and a generalist feeding behaviour demonstrate that G. callidus is an equilibrium life strategist. In comparison with other species, the life-history traits of G. callidus from irrigation impoundments resemble those of other freshwater goby species, some of which are global invaders.
- Full Text:
- Date Issued: 2016
- Authors: Mofu, Lubabalo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/1136 , vital:20023
- Description: The River Goby Glossogobius callidus (Smith, 1937) is a native abundant fish in both freshwater and estuarine habitats in the Cape Fold Ecoregion, yet little information is available on its life-history. This study aims to contribute to knowledge on the age and growth, reproductive biology and the diet and feeding habits of G. callidus in irrigation impoundments. Glossogobius callidus was sampled monthly from August 2013 till March 2015; from the irrigation ponds in the Sundays River Valley, Eastern Cape Province, South Africa. To determine sex, gonads were visually assessed under a dissecting microscope to confirm the sex based on the shape of the urogenital papillae. Fish were then dissected to confirm sex and gonads were categorised into five macroscopic stages which were histologically validated. Microscopic stages of gonadal development were discerned based on nuclear and cytoplasmic characteristics of the oocyte or sperm. Ovaries and sperms were assigned stages based on the most advanced type of oocyte present. In total 2054 fishes ranging in length from 21.1 mm to 137.2 mm TL were sampled. The sex ratio (1.1 males: 1 females) did not differ from unity (x2 = 0.027, df = 1, p = 0.87). Length at 50% maturity (Lm) was 70 mm TL females and 72 mm TL for males. Spawning season was mid-spring and mid-summer and mean ± S.D absolute fecundity was estimated at 1028.2 ± 131.7 ova/fish. Relative fecundity (number of vitellogenic oocytes per gram of eviscerated fish mass) were estimated at 50 ± 18 ova/fish gram. Otoliths from 560 fish were used for ageing. Growth zone deposition rate was validated using edge analysis. As a unimodal periodic regression model best described the temporal proportion of opaque zone deposition on the edge of otoliths over a one-year period, growth zone deposition rate was validated as annual. The oldest female fish was a 4-year old 84.4 mm TL fish and the oldest male was a 7-year old 100.5 mm TL fish. The length-at-age for the entire population of 560 G. callidus provided von Bertalanffy parameters of Lt = 92 (1 - e -0.58(t + 0.4)) mm TL for the entire population, Lt = 70 (1 - e -1.8 (t + 0.06)) mm TL for males and Lt = 65 (1 - e -1.8 (t + 0.05)) mm TL for females. Converting length at maturity to age at maturity demonstrated that G. callidus attained maturity at an age of 2-years. Growth performance described using the phi-prime index showed that G. callidus had lower growth performance compared to the invasive Neogobius melanostomus. Using age structure, natural mortality was estimated at 1.31 yr-1 using catch curve analysis. Diet of G. callidus comprised of ten taxonomic groups. Among these, aquatic invertebrates were the most diverse group but while relative contribution of the dietary components varied across all size classes and seasons, the key prey items were consistently found in all size classes. These were Diptera, Hemiptera, Trichoptera, Odonata, Cladocera, Copepoda, Hydracarina, Amphipoda, Crustacea, and Mollusca. While dietary differences were observed between the size classes and throughout the seasons, G. callidus can be regarded as a generalist feeder preying on an array of different species. Given its abundance and diet, I suggest that G. callidus contribute considerably to the invertebrate predation pressure in these artificial aquatic environments in an arid region. In summary, medium fecundity, fast growth, moderate maturity, and a generalist feeding behaviour demonstrate that G. callidus is an equilibrium life strategist. In comparison with other species, the life-history traits of G. callidus from irrigation impoundments resemble those of other freshwater goby species, some of which are global invaders.
- Full Text:
- Date Issued: 2016
Geochemical exploration for copper - cobalt in the Democratic Republic of Congo, Central African Copperbelt: a case study on PR851
- Authors: Katombe-Kisumbule, Paul
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3035 , vital:20354
- Description: The PR851 licence area is located at about 80 km west from the town of Likasi in the district of Haut-Katanga and 175 km north-west of Lubumbashi, the capital city of Katanga Province in the Democratic Republic of Congo. The exploration licence was granted by the government of the Democratic Republic of Congo to First Quantum Minerals Ltd through its Congolese subsidiary Compagnie Minière de Sakania Sprl (CoMiSa Sprl) under certificate N˚ CAMI/CR/70/2003 on the 10th of October 2003 for a period of 5 years with a possibility of renewal for 3 years in respect to the new Congolese mining code. The PR851 area lies on fragments of Mines Subgroup rocks of the Roan Group in the Congolese Copperbelt where most of the Cu-Co and stratiform-stratabound deposits such as Kipushi, Ruashi-Etoile, Kinsevere, Kipoi, Luishya, Luswishi, Shituru, Kamoya, Kambove, Tenke- Fungurume, Shinkolobwe, Swambo, Mindingi and Kamoto among others are found. During the 20th century, the Union Minière du Haut Katanga (U.M.H.K.) undertook mineral exploration in the Congolese Copperbelt and numerous copper- and cobalt-occurrences were identified (for instance Kibamba copper occurrence in PR851 area). From 2003, the Compagnie Minière de Sakania Sprl initiated a grassroots exploration program in PR851 area and geochemical exploration survey as one of the mineral exploration tools was implemented to aim at detecting copper and cobalt concentration in soil. The B horizon of the thick tropical soil in the area was sampled and soil samples were sent to Genalysis laboratories in Johannesburg, Republic of South Africa for main chemical analysis of Cu and Co only, whereas 10% of analyzed samples were dispatched to Perth, Western Australia for quality control analysis. Thresholds for anomalies of copper and cobalt were defined by literature comparison, standard deviations and spatial analysis. The anomalies were tested at a later stage by reverse circulation / diamond drilling during the year of 2005 to 2008 and the Cu-Co resources were estimated by Digital Mining Services of Harare, Zimbabwe in the year of 2008. Geological logging of chips from reverse circulation and diamond drill cores revealed that copper mineralization is represented by malachite, chrysocolla, chalcopyrite and bornite whereas cobalt mineralization appeared in form of heterogenite. The source of supergene mineralization remains unknown. Recommendations have been made to undertake more geological exploration work in order to fully investigate the geological setting and structural architecture of the region, which may result in a better understanding of the Cu-Co mineralization system and ore genesis. The latter has been no consensus up-to-date and different theories have been proposed to discuss the ore genesis, including syn- and dia- genetic, synorogenic and sulphide remobilization to late-to-post- orogenic Cu-Zn-Pb Kipushi-type deposit. However, geological observations favored that the diagenetic and syngenetic models are applicable to numerous deposits in the Central African Copperbelt.
- Full Text:
- Date Issued: 2016
- Authors: Katombe-Kisumbule, Paul
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3035 , vital:20354
- Description: The PR851 licence area is located at about 80 km west from the town of Likasi in the district of Haut-Katanga and 175 km north-west of Lubumbashi, the capital city of Katanga Province in the Democratic Republic of Congo. The exploration licence was granted by the government of the Democratic Republic of Congo to First Quantum Minerals Ltd through its Congolese subsidiary Compagnie Minière de Sakania Sprl (CoMiSa Sprl) under certificate N˚ CAMI/CR/70/2003 on the 10th of October 2003 for a period of 5 years with a possibility of renewal for 3 years in respect to the new Congolese mining code. The PR851 area lies on fragments of Mines Subgroup rocks of the Roan Group in the Congolese Copperbelt where most of the Cu-Co and stratiform-stratabound deposits such as Kipushi, Ruashi-Etoile, Kinsevere, Kipoi, Luishya, Luswishi, Shituru, Kamoya, Kambove, Tenke- Fungurume, Shinkolobwe, Swambo, Mindingi and Kamoto among others are found. During the 20th century, the Union Minière du Haut Katanga (U.M.H.K.) undertook mineral exploration in the Congolese Copperbelt and numerous copper- and cobalt-occurrences were identified (for instance Kibamba copper occurrence in PR851 area). From 2003, the Compagnie Minière de Sakania Sprl initiated a grassroots exploration program in PR851 area and geochemical exploration survey as one of the mineral exploration tools was implemented to aim at detecting copper and cobalt concentration in soil. The B horizon of the thick tropical soil in the area was sampled and soil samples were sent to Genalysis laboratories in Johannesburg, Republic of South Africa for main chemical analysis of Cu and Co only, whereas 10% of analyzed samples were dispatched to Perth, Western Australia for quality control analysis. Thresholds for anomalies of copper and cobalt were defined by literature comparison, standard deviations and spatial analysis. The anomalies were tested at a later stage by reverse circulation / diamond drilling during the year of 2005 to 2008 and the Cu-Co resources were estimated by Digital Mining Services of Harare, Zimbabwe in the year of 2008. Geological logging of chips from reverse circulation and diamond drill cores revealed that copper mineralization is represented by malachite, chrysocolla, chalcopyrite and bornite whereas cobalt mineralization appeared in form of heterogenite. The source of supergene mineralization remains unknown. Recommendations have been made to undertake more geological exploration work in order to fully investigate the geological setting and structural architecture of the region, which may result in a better understanding of the Cu-Co mineralization system and ore genesis. The latter has been no consensus up-to-date and different theories have been proposed to discuss the ore genesis, including syn- and dia- genetic, synorogenic and sulphide remobilization to late-to-post- orogenic Cu-Zn-Pb Kipushi-type deposit. However, geological observations favored that the diagenetic and syngenetic models are applicable to numerous deposits in the Central African Copperbelt.
- Full Text:
- Date Issued: 2016
An assessment of the invasion state and fisheries suitability in four dams and a natural lake in the Western Cape, South Africa
- Authors: Dredge, Brendon Neville
- Date: 2016
- Subjects: Introduced organisms Fishery management -- South Africa -- Western Cape Fishes -- Conservation -- South Africa -- Western Cape Introduced fishes -- South Africa -- Western Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/69114 , vital:29391
- Description: Despite more than a century of introductions, fish invasions are poorly understood in South Africa. This thesis aims to: (1) provide baseline information on the fish fauna in five inland water bodies (four dams: Theewaterskloof, Clanwilliam, Quaggaskloof, and Voelvlei and a natural lake, Groenvlei) in the Western Cape; (2) determine which species were established and provide a baseline of abundance and size structure for monitoring and, (3) make preliminary recommendations for a fishery. First, criteria for determining establishment were developed by undertaking a full biological assessment of bluegill Lepomis macrochirus, a data-poor species in South Africa. This included the assessment of growth rate using validated estimates of age derived from otoliths in two dams (Clanwilliam Dam: Lt=335.9(1−eo.113(t+1.06)); Groenvlei: Lt=287.2(1-e−0.171(t+0.54)). Length at 50% maturity was similar for males (146 mmFL) and females (147 mmFL). Length frequency analysis combined with data on the age and growth and reproduction demonstrated that established populations of bluegill contained several age cohorts as well as mature and juvenile fishes. A meta-analysis was conducted to: (1) compile a list of initial stocking dates for all alien fishes in each waterbody and (2) use angler tournament data to assess for additional species and persistence over time. Data from the meta-analysis was combined with ichthyological survey data to determine establishment success. These data demonstrated that nine non-native species had established in the five water bodies. Clanwilliam Dam had the largest number (7) of non-native species established, Groenvlei (5) and Theewaterskloof, Brandvlei and Voelvlei the lowest with four species. The state of invasion of each species in the province showed that all nine species are categorised as invasive. This information on establishment was then integrated into a framework for rapidly determining what type of fishery would be most suitable for the 5 waterbodies. Potential yield was estimated using morphoedaphic models, were 260 t/yr for Theewaterskloof Dam, 93 t/yr for Voelvlei, 81 t/yr for Brandvlei, 59 t/yr for Groenvlei and 55 t/yr for Groenvlei. The CPUE for Groenvlei was the highest in gill nets with 1.32 ± 1.94 kg/h, then Voelvlei 1.05 ± 1.34 kg/h followed by Brandvlei (0.84 ± 1.48 kg/h), Clanwilliam (0.71 ± 1.55 kg/h) and lastly Theewaterskloof (0.36 ± 0.41 kg/h). Only Theewaterskloof could produce in excess of 100 t/yr but less than 400 t/yr of fish making it suitable for a small scale fishery. The CPUE values of Theewaterskloof were the lowest and a gill net fishery on this water body may not produce sufficient fish for the fishery. All water bodies had a high recreational angling usage, the opening of a small scale fishery could be detrimental to this industry. Subsistence and recreational anglers should be promoted with the possibility of a long line industry targeting Clarias gariepinus.
- Full Text:
- Date Issued: 2016
- Authors: Dredge, Brendon Neville
- Date: 2016
- Subjects: Introduced organisms Fishery management -- South Africa -- Western Cape Fishes -- Conservation -- South Africa -- Western Cape Introduced fishes -- South Africa -- Western Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/69114 , vital:29391
- Description: Despite more than a century of introductions, fish invasions are poorly understood in South Africa. This thesis aims to: (1) provide baseline information on the fish fauna in five inland water bodies (four dams: Theewaterskloof, Clanwilliam, Quaggaskloof, and Voelvlei and a natural lake, Groenvlei) in the Western Cape; (2) determine which species were established and provide a baseline of abundance and size structure for monitoring and, (3) make preliminary recommendations for a fishery. First, criteria for determining establishment were developed by undertaking a full biological assessment of bluegill Lepomis macrochirus, a data-poor species in South Africa. This included the assessment of growth rate using validated estimates of age derived from otoliths in two dams (Clanwilliam Dam: Lt=335.9(1−eo.113(t+1.06)); Groenvlei: Lt=287.2(1-e−0.171(t+0.54)). Length at 50% maturity was similar for males (146 mmFL) and females (147 mmFL). Length frequency analysis combined with data on the age and growth and reproduction demonstrated that established populations of bluegill contained several age cohorts as well as mature and juvenile fishes. A meta-analysis was conducted to: (1) compile a list of initial stocking dates for all alien fishes in each waterbody and (2) use angler tournament data to assess for additional species and persistence over time. Data from the meta-analysis was combined with ichthyological survey data to determine establishment success. These data demonstrated that nine non-native species had established in the five water bodies. Clanwilliam Dam had the largest number (7) of non-native species established, Groenvlei (5) and Theewaterskloof, Brandvlei and Voelvlei the lowest with four species. The state of invasion of each species in the province showed that all nine species are categorised as invasive. This information on establishment was then integrated into a framework for rapidly determining what type of fishery would be most suitable for the 5 waterbodies. Potential yield was estimated using morphoedaphic models, were 260 t/yr for Theewaterskloof Dam, 93 t/yr for Voelvlei, 81 t/yr for Brandvlei, 59 t/yr for Groenvlei and 55 t/yr for Groenvlei. The CPUE for Groenvlei was the highest in gill nets with 1.32 ± 1.94 kg/h, then Voelvlei 1.05 ± 1.34 kg/h followed by Brandvlei (0.84 ± 1.48 kg/h), Clanwilliam (0.71 ± 1.55 kg/h) and lastly Theewaterskloof (0.36 ± 0.41 kg/h). Only Theewaterskloof could produce in excess of 100 t/yr but less than 400 t/yr of fish making it suitable for a small scale fishery. The CPUE values of Theewaterskloof were the lowest and a gill net fishery on this water body may not produce sufficient fish for the fishery. All water bodies had a high recreational angling usage, the opening of a small scale fishery could be detrimental to this industry. Subsistence and recreational anglers should be promoted with the possibility of a long line industry targeting Clarias gariepinus.
- Full Text:
- Date Issued: 2016
Local versus landscape effects of bush encroachment on plant available light, soil moisture, frost occurrence and herbaceous productivity and composition
- Authors: Klopper, Chrisna
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3137 , vital:20376
- Description: Bush encroachment is a global phenomenon and a pressing concern for South Africa rangelands. The expansion and increase in density of Vachellia karroo (hereafter referred to aAcacia karroo) has been documented in the Eastern Cape and KwaZulu Natal in South Africa. This increase in woody species in savannas is often at the expense of grass cover and thus is causing concern about how this will impact agriculture due to possible loss of grazing capacity. To understand the impacts of bush encroachment the effect of trees on their micro-climate and abiotic factors and in turn on the herbaceous layer needs to be examined. The objective of this study was to quantify the effect of Acacia karroo encroachment on the light environment, soil moisture and frost occurrence in the sub-canopy and inter-canopy micro-habitats, and how these changes affected herbaceous player productivity and composition. Another question that is being addressed here is whether, and how, the local tree effects scale up in the landscape and whether prediction can be made based on the effects of individual trees. The study was undertaken on a farm, Endwell, in the Smaldeel, Eastern Cape. At the study site, a semi-arid savanna, Acacia karroo has been encroaching since the 1980’s. The study was conducted at three scales: landscape, stand-wide and local scale. The landscape scale was represented by four areas with 0, 21, 45 and 72% tree canopy cover, the stand-wide scale consisted of transects with varying percentages of tree canopy cover within each of the four levels of encroachment. The local scale was represented by the sub-canopy and inter-canopy environment to test the effect of trees. At the local scale plant available light and soil moisture were lower in the sub-canopy than intercanopy regions, with leaf area index being higher in the sub-canopy. This local negative effect of the tree canopy on light and soil moisture in the sub-canopy did not scale up predictably in the landscape. At the stand-wide scale light in the inter-canopy was reduced as shading increased. Frost was excluded from under the canopies and frost incidence decreased at higher tree cover. Grass productivity was reduced in the sub-canopy, possibly due to lower light and soil moisture. Overall biomass increased from the low to medium level of encroachment but lowest at the high level of encroachment. Grass composition and cover was only slightly affected by tree canopies cover and C4 grass species were still present in the sub-canopy and at lower light environments. At the levels of Acacia karroo encroachment encountered at this study site, it seems unlikely that palatable or desirable C4 would be excluded from the system and that a shift from C4 to shade-tolerant species would occur. This is due to tree canopies at the site not reducing light to such anextent that they would outcompete grasses, and likely the very low grazing pressure at thesesites. Herbaceous biomass at these sites were still sufficient to carry a fire in the inter-canopyregion and sufficient grazing for herbivores. In the sub-canopy region fires will be excluded thus with higher the portion of sub-canopy areas increasing at the high levels of encroachment they may interrupt fire spread. Thus it was concluded that Acacia karroo encroachment up to 45% tree cover is currently not creating negative feedback on herbivory, but low stocking rates appear to be key to maintain this.
- Full Text:
- Date Issued: 2016
- Authors: Klopper, Chrisna
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3137 , vital:20376
- Description: Bush encroachment is a global phenomenon and a pressing concern for South Africa rangelands. The expansion and increase in density of Vachellia karroo (hereafter referred to aAcacia karroo) has been documented in the Eastern Cape and KwaZulu Natal in South Africa. This increase in woody species in savannas is often at the expense of grass cover and thus is causing concern about how this will impact agriculture due to possible loss of grazing capacity. To understand the impacts of bush encroachment the effect of trees on their micro-climate and abiotic factors and in turn on the herbaceous layer needs to be examined. The objective of this study was to quantify the effect of Acacia karroo encroachment on the light environment, soil moisture and frost occurrence in the sub-canopy and inter-canopy micro-habitats, and how these changes affected herbaceous player productivity and composition. Another question that is being addressed here is whether, and how, the local tree effects scale up in the landscape and whether prediction can be made based on the effects of individual trees. The study was undertaken on a farm, Endwell, in the Smaldeel, Eastern Cape. At the study site, a semi-arid savanna, Acacia karroo has been encroaching since the 1980’s. The study was conducted at three scales: landscape, stand-wide and local scale. The landscape scale was represented by four areas with 0, 21, 45 and 72% tree canopy cover, the stand-wide scale consisted of transects with varying percentages of tree canopy cover within each of the four levels of encroachment. The local scale was represented by the sub-canopy and inter-canopy environment to test the effect of trees. At the local scale plant available light and soil moisture were lower in the sub-canopy than intercanopy regions, with leaf area index being higher in the sub-canopy. This local negative effect of the tree canopy on light and soil moisture in the sub-canopy did not scale up predictably in the landscape. At the stand-wide scale light in the inter-canopy was reduced as shading increased. Frost was excluded from under the canopies and frost incidence decreased at higher tree cover. Grass productivity was reduced in the sub-canopy, possibly due to lower light and soil moisture. Overall biomass increased from the low to medium level of encroachment but lowest at the high level of encroachment. Grass composition and cover was only slightly affected by tree canopies cover and C4 grass species were still present in the sub-canopy and at lower light environments. At the levels of Acacia karroo encroachment encountered at this study site, it seems unlikely that palatable or desirable C4 would be excluded from the system and that a shift from C4 to shade-tolerant species would occur. This is due to tree canopies at the site not reducing light to such anextent that they would outcompete grasses, and likely the very low grazing pressure at thesesites. Herbaceous biomass at these sites were still sufficient to carry a fire in the inter-canopyregion and sufficient grazing for herbivores. In the sub-canopy region fires will be excluded thus with higher the portion of sub-canopy areas increasing at the high levels of encroachment they may interrupt fire spread. Thus it was concluded that Acacia karroo encroachment up to 45% tree cover is currently not creating negative feedback on herbivory, but low stocking rates appear to be key to maintain this.
- Full Text:
- Date Issued: 2016
Development of an enzyme-synergy based bioreactor system for the beneficiation of apple pomace lignocellulosic waste
- Authors: Abboo, Sagaran
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/315 , vital:19947
- Description: Due to the finite supply of non-renewable fossil fuels, agro-industrial wastes are identified as alternate, renewable sources for energy supply. Large amounts of fruit waste are generated in South Africa due to fruit juice and wine processing from apples, grapes and citrus fruit. Apple pomace is the solid residue that is left over after juice, cider and wine processing and constitutes between 25-30% of the total fruit. On a global scale millions of tonnes of apple pomace are produced; between 2006-2007 over 46 million tonnes were produced. In South Africa a total production of 244 469 tonnes were produced during the 2011- 2012 season. Initially, apple pomace was regarded as a waste by-product used for animal feed and compost in soil, however presently it is considered a source of dietary fiber and natural antioxidants like polyphenols. In addition, apple pomace has a high carbohydrate content and can be enzymatically hydrolysed to produce sugar monomers which, in turn, can be fermented by yeasts to produce bioethanol. The polyphenols present in apple pomace can be used for their health properties, and the bioethanol can be used as a replacement for fossil fuel. Apple pomace is lignocellulosic in nature and consists of hemicellulose, cellulose, lignin and pectin. A combination of enzymes such as cellulases, hemicellulases, pectinases and lignases are required to operate in synergy for the degradation of lignocellulosic biomass. This is due to the recalcitrant nature of lignocellulose. This study investigated the degradation of apple pomace using a combination of commercially obtained enzyme cocktails viz. Viscozyme L , Celluclast 1.5L and Novozyme 188. The commercial enzymes Viscozyme L and Celluclast 1.5L were added in a ratio of 1:1 (50%:50%). The final concentrations of the enzymes were 0.019 mg/ml each. Novozyme 188 was added to provide a final concentration of 0.0024 mg/ml. A novel cost effective 20L bioreactor was designed, constructed and implemented for the degradation of apple pomace to produce value added products. The hydrolysis of the apple pomace was performed initially in 1 L flasks (batch fed) and, once optimized, scaled up to a 20 L bioreactor in batch mode. The bioreactors were operated at room temperature (22 ± 2ºC) and in an unbuffered system. The sugars released were detected and quantified using an optimized validated HPLC method established in this study. The sugars released in the bioreactors were mainly glucose, galactose, arabinose, cellobiose and fructose. The polyphenols released in this study were gallic acid, catechin, epicatechin, chlorogenic acid, rutin and phloridzin, which have a number of health benefits. The simultaneous analyses of the polyphenols were performed using a newly developed and validated HPLC method established in this study. This method was developed to detect nine polyphenols simultaneously. The two HPLC methods developed and validated in this study for the analysis of sugars and polyphenols demonstrated good accuracy, precision, reproducibility, linearity, robustness and sensitivity. Both analytical methods were validated according to the International Convention on Harmonization (ICH). The HPLC parameters for sugar analysis were: refractive index (RI) as the detection mode, the stationary phase was a ligand-exchange sugar column (Shodex SP0810) and an aqueous mobile phase in isocratic mode was used. The HPLC method for polyphenols employed UV diode array detection (DAD) as the detection mode, a reverse phase column as the stationary phase and a mobile phase of consisting of 0.01 M phosphoric acid in water and 100% methanol using gradient elution mode. The highest concentrations of sugars released in the novel 20 L bioreactor with 20% apple pomace (w/v) substrate loading were as follow: glucose (6.5 mg/ml), followed by galactose (2.1 mg/ml), arabinose (1.4 mg/ml), cellobiose (0.7 mg/ml) and fructose (0.5 mg/ml). The amounts of polyphenols released at 20% (w/v) apple pomace substrate were epicatechin (0.01 mg/ml), catechin (0.002 mg/ml), rutin (0.03 mg/ml), chlorogenic acid (0.002 mg/ml) and gallic acid 0.01 (mg/ml). Two mathematical models were developed in this study for kinetic analysis of lignocellulose (apple pomace) hydrolysis in the novel 20 L bioreactor, using the experimental data generated by the above HPLC analyses. The first model, modelling with regression, defines the hydrolysis of the sugars glucose, galactose, cellobiose and arabinose produced in the novel 20 L bioreactor at 5%, 10%, 15% and 20% (w/v) substrate concentrations. The regression model describes the sugars produced in the 20 L bioreactor by minimizing the error of the sugars released by finding a value for K which minimises the function which computes the sum of squares of errors between the solution curves and the data points. The second, more complex, model developed in this study used a system of differential equations model (ODE). This model solved the system by using a numerical method, such as the Runge-Kutta method, then fitted the solution curves to the data. Both models simulated (and had the ability to predict) the production of sugars in the novel 20 L bioreactor for apple pomace hydrolysis. These two models also revealed the time at which the maximum amount of sugars were released, which revealed the optimum time to run the 20 L bioreactor in order to be more cost effective. The optimum time for maximum glucose (the main sugar used in fermentation for biofuel production) release was determined to be around 60 h. The ODE model, in addition, determined the rate at which the substrate became depleted, as well as the rate at which the enzymes became deactivated for the various substrate loadings in the 20 L bioreactor. A third model was developed to determine the optimal running cost of the bioreactor which incorporated the substrate loading and the amount of glucose (g/L) produced. The novel 20 L bioreactor constructed from cost effective materials demonstrated that agro-industrial waste can be converted to value-added products by lignocellolytic enzymes. The sugars released from apple pomace can be used in biofuel production and the polyphenols as food supplements and nutraceuticals for health benefits. This novel study contributes to agro-industrial waste beneficiation via fuel production. In addition, using agro-industrial waste for the generation of value added products (instead of mere disposal) will help prevent environmental pollution.
- Full Text:
- Date Issued: 2016
- Authors: Abboo, Sagaran
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/315 , vital:19947
- Description: Due to the finite supply of non-renewable fossil fuels, agro-industrial wastes are identified as alternate, renewable sources for energy supply. Large amounts of fruit waste are generated in South Africa due to fruit juice and wine processing from apples, grapes and citrus fruit. Apple pomace is the solid residue that is left over after juice, cider and wine processing and constitutes between 25-30% of the total fruit. On a global scale millions of tonnes of apple pomace are produced; between 2006-2007 over 46 million tonnes were produced. In South Africa a total production of 244 469 tonnes were produced during the 2011- 2012 season. Initially, apple pomace was regarded as a waste by-product used for animal feed and compost in soil, however presently it is considered a source of dietary fiber and natural antioxidants like polyphenols. In addition, apple pomace has a high carbohydrate content and can be enzymatically hydrolysed to produce sugar monomers which, in turn, can be fermented by yeasts to produce bioethanol. The polyphenols present in apple pomace can be used for their health properties, and the bioethanol can be used as a replacement for fossil fuel. Apple pomace is lignocellulosic in nature and consists of hemicellulose, cellulose, lignin and pectin. A combination of enzymes such as cellulases, hemicellulases, pectinases and lignases are required to operate in synergy for the degradation of lignocellulosic biomass. This is due to the recalcitrant nature of lignocellulose. This study investigated the degradation of apple pomace using a combination of commercially obtained enzyme cocktails viz. Viscozyme L , Celluclast 1.5L and Novozyme 188. The commercial enzymes Viscozyme L and Celluclast 1.5L were added in a ratio of 1:1 (50%:50%). The final concentrations of the enzymes were 0.019 mg/ml each. Novozyme 188 was added to provide a final concentration of 0.0024 mg/ml. A novel cost effective 20L bioreactor was designed, constructed and implemented for the degradation of apple pomace to produce value added products. The hydrolysis of the apple pomace was performed initially in 1 L flasks (batch fed) and, once optimized, scaled up to a 20 L bioreactor in batch mode. The bioreactors were operated at room temperature (22 ± 2ºC) and in an unbuffered system. The sugars released were detected and quantified using an optimized validated HPLC method established in this study. The sugars released in the bioreactors were mainly glucose, galactose, arabinose, cellobiose and fructose. The polyphenols released in this study were gallic acid, catechin, epicatechin, chlorogenic acid, rutin and phloridzin, which have a number of health benefits. The simultaneous analyses of the polyphenols were performed using a newly developed and validated HPLC method established in this study. This method was developed to detect nine polyphenols simultaneously. The two HPLC methods developed and validated in this study for the analysis of sugars and polyphenols demonstrated good accuracy, precision, reproducibility, linearity, robustness and sensitivity. Both analytical methods were validated according to the International Convention on Harmonization (ICH). The HPLC parameters for sugar analysis were: refractive index (RI) as the detection mode, the stationary phase was a ligand-exchange sugar column (Shodex SP0810) and an aqueous mobile phase in isocratic mode was used. The HPLC method for polyphenols employed UV diode array detection (DAD) as the detection mode, a reverse phase column as the stationary phase and a mobile phase of consisting of 0.01 M phosphoric acid in water and 100% methanol using gradient elution mode. The highest concentrations of sugars released in the novel 20 L bioreactor with 20% apple pomace (w/v) substrate loading were as follow: glucose (6.5 mg/ml), followed by galactose (2.1 mg/ml), arabinose (1.4 mg/ml), cellobiose (0.7 mg/ml) and fructose (0.5 mg/ml). The amounts of polyphenols released at 20% (w/v) apple pomace substrate were epicatechin (0.01 mg/ml), catechin (0.002 mg/ml), rutin (0.03 mg/ml), chlorogenic acid (0.002 mg/ml) and gallic acid 0.01 (mg/ml). Two mathematical models were developed in this study for kinetic analysis of lignocellulose (apple pomace) hydrolysis in the novel 20 L bioreactor, using the experimental data generated by the above HPLC analyses. The first model, modelling with regression, defines the hydrolysis of the sugars glucose, galactose, cellobiose and arabinose produced in the novel 20 L bioreactor at 5%, 10%, 15% and 20% (w/v) substrate concentrations. The regression model describes the sugars produced in the 20 L bioreactor by minimizing the error of the sugars released by finding a value for K which minimises the function which computes the sum of squares of errors between the solution curves and the data points. The second, more complex, model developed in this study used a system of differential equations model (ODE). This model solved the system by using a numerical method, such as the Runge-Kutta method, then fitted the solution curves to the data. Both models simulated (and had the ability to predict) the production of sugars in the novel 20 L bioreactor for apple pomace hydrolysis. These two models also revealed the time at which the maximum amount of sugars were released, which revealed the optimum time to run the 20 L bioreactor in order to be more cost effective. The optimum time for maximum glucose (the main sugar used in fermentation for biofuel production) release was determined to be around 60 h. The ODE model, in addition, determined the rate at which the substrate became depleted, as well as the rate at which the enzymes became deactivated for the various substrate loadings in the 20 L bioreactor. A third model was developed to determine the optimal running cost of the bioreactor which incorporated the substrate loading and the amount of glucose (g/L) produced. The novel 20 L bioreactor constructed from cost effective materials demonstrated that agro-industrial waste can be converted to value-added products by lignocellolytic enzymes. The sugars released from apple pomace can be used in biofuel production and the polyphenols as food supplements and nutraceuticals for health benefits. This novel study contributes to agro-industrial waste beneficiation via fuel production. In addition, using agro-industrial waste for the generation of value added products (instead of mere disposal) will help prevent environmental pollution.
- Full Text:
- Date Issued: 2016
Exploring the relationship between leadership styles and quality of work life: a case study of a Chinese- South African joint venture
- Authors: Handley, Rayne Cyla
- Date: 2016
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10962/4058 , vital:20597
- Description: This thesis employs a phenomenological qualitative research methodology to explore its research aims and objectives. It focuses on describing the various leadership styles and quality of work life (QWL) levels with reference to the Chinese-South African intercultural context. By doing so, it answers a primary exploratory research question; how does a particular leadership style influence QWL. By answering this question, this study will contribute to the understanding of the dynamics of Chinese organisations in South Africa. Furthermore, it will assist in creating greater intercultural synergy within the respective organisation. In order to explore the research question related to leadership and QWL, the thesis will begin by defining and describing the two concepts indigenously. In light of the research gap pertaining to African intercultural managerial contexts, as well as the rising Chinese and South Africa intercultural business environments, this case study demonstrates how leadership style is an important determining factor in QWL levels, both of the leaders as well as leader-raters within an intercultural context. China is South Africa’s largest trading partner and the signing of new agreements in 2015 will lead to enhanced China-Africa engagement at the macro and organisational level. China’s increasing engagement in both Africa and South Africa has been widely covered, but non-pejorative empirical research is needed to shed light on the organisational manifestations of China’s engagement. The study was conducted within the mining sector which is a key component of Chinese investment in, and trade with, South Africa. Through an in-depth content analysis which draws on coding and thematic concerns, quantification and description, this study finds that leaders directly influence QWL through relationships with their followers. Moreover, leaders indirectly have a bearing on QWL through the influence they have on organisational and work environment factors. Another finding is that leaders are inclined to describing higher levels of QWL and more transformational leadership styles. In addition, it is shown that executives (irrespective of leader or leader-rater status) were more likely to describe a high level of QWL and transformational leadership behaviours when compared to skilled level participants while the semi-skilled participants where least likely of all. Finally, it was found that the nature of the relationship between a leader and a follower is influenced by whether the said leader is a direct supervisor or if there is a large organisational level ‘gap’ between a leader and a leader- rater. It can also be said that leaders at higher organisational levels are expected to exhibit different leadership behaviours and meet different needs. Overall this study suggests that leaders need to be aware of the way in which the intercultural context can influence perceptions of subjective phenomena such as leadership effectiveness and QWL. The study concludes that leaders directly and indirectly play a key role in determining need satisfaction and QWL levels. To that extent, they ought to strategically adopt leadership practices that enhance need satisfaction and wellbeing in the workplace. Wellbeing and employee satisfaction are increasingly gaining importance within theory and literature related to QWL and has, importantly, also been shown to influence workplace attitudes and behaviours.
- Full Text:
- Date Issued: 2016
- Authors: Handley, Rayne Cyla
- Date: 2016
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10962/4058 , vital:20597
- Description: This thesis employs a phenomenological qualitative research methodology to explore its research aims and objectives. It focuses on describing the various leadership styles and quality of work life (QWL) levels with reference to the Chinese-South African intercultural context. By doing so, it answers a primary exploratory research question; how does a particular leadership style influence QWL. By answering this question, this study will contribute to the understanding of the dynamics of Chinese organisations in South Africa. Furthermore, it will assist in creating greater intercultural synergy within the respective organisation. In order to explore the research question related to leadership and QWL, the thesis will begin by defining and describing the two concepts indigenously. In light of the research gap pertaining to African intercultural managerial contexts, as well as the rising Chinese and South Africa intercultural business environments, this case study demonstrates how leadership style is an important determining factor in QWL levels, both of the leaders as well as leader-raters within an intercultural context. China is South Africa’s largest trading partner and the signing of new agreements in 2015 will lead to enhanced China-Africa engagement at the macro and organisational level. China’s increasing engagement in both Africa and South Africa has been widely covered, but non-pejorative empirical research is needed to shed light on the organisational manifestations of China’s engagement. The study was conducted within the mining sector which is a key component of Chinese investment in, and trade with, South Africa. Through an in-depth content analysis which draws on coding and thematic concerns, quantification and description, this study finds that leaders directly influence QWL through relationships with their followers. Moreover, leaders indirectly have a bearing on QWL through the influence they have on organisational and work environment factors. Another finding is that leaders are inclined to describing higher levels of QWL and more transformational leadership styles. In addition, it is shown that executives (irrespective of leader or leader-rater status) were more likely to describe a high level of QWL and transformational leadership behaviours when compared to skilled level participants while the semi-skilled participants where least likely of all. Finally, it was found that the nature of the relationship between a leader and a follower is influenced by whether the said leader is a direct supervisor or if there is a large organisational level ‘gap’ between a leader and a leader- rater. It can also be said that leaders at higher organisational levels are expected to exhibit different leadership behaviours and meet different needs. Overall this study suggests that leaders need to be aware of the way in which the intercultural context can influence perceptions of subjective phenomena such as leadership effectiveness and QWL. The study concludes that leaders directly and indirectly play a key role in determining need satisfaction and QWL levels. To that extent, they ought to strategically adopt leadership practices that enhance need satisfaction and wellbeing in the workplace. Wellbeing and employee satisfaction are increasingly gaining importance within theory and literature related to QWL and has, importantly, also been shown to influence workplace attitudes and behaviours.
- Full Text:
- Date Issued: 2016
Investigating soil microbial interactions of Portulacaria afra
- Authors: Fulmaka, Aviwe
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/54598 , vital:26592
- Description: Portulacaria afra commonly known as Spekboom contributes significantly to carbon sequestration and has been widely planted in degraded areas of the Eastern Cape. Approximately 50% of planted cuttings do not survive although the cause of this decline is unknown. Like many indigenous plants, Spekboom forms a symbiotic relationship with mycorrhizal fungi and the interaction with rhizobacteria may enhance and improve plant growth and establishment. This study aims to investigate these relationships which will include a survey of the arbuscular mycorrhizal (AM) fungal populations associated with Spekboom, determination of the causal agent of Spekboom decline, isolation and identification of the associated rhizobacteria and investigation of their plant growth promotion properties and assessing the ability of arbuscular mycorrhizal fungi and selected rhizobacteria to enhance establishment and growth of Spekboom. Soil and root samples from selected trial sites were used to assess AM fungal spore abundance and colonisation; isolation, characterization, and identification of rhizobacteria and determine the interaction of the microbes on Spekboom growth and tolerance to Fusarium. AM spore abundance and percentage root colonisation did not differ between the three Spekboom plots. Molecular analyses of the SSU region from the plots showed 4 families of AM fungi and were identified as Ambisporaceae, Glomeraceae, Claroideoglomeraceae and Paraglomeraceae. A suspected Fusarium pathogen was isolated and molecularly identified. Pathogenicity tests indicated reduced Spekboom growth with poor root development. Thirty four rhizobacterial isolates were tested for various plant growth promoting abilities. Of these, 6 were able to produce IAA which may promote plant root growth, 27 siderophores and 23 were phosphate solubilisers. Bacterial isolates were molecularly identified to be from various species of Bacillus, with some Arthrobacter, Enterobacter, Pseudomonas and Microbacterium. Inoculation of Spekboom cuttings with mycorrhizal fungi and selected rhizobacterial isolates significantly improved shoot height. Spekboom cuttings challenged with Fusarium and inoculated with mycorrhizal fungi and two rhizobacterial isolates significantly improved growth. The inoculation of cuttings in the nursery with mycorrhizal fungi and selected rhizobacteria is recommended prior to establishing Spekboom in the field.
- Full Text:
- Date Issued: 2016
- Authors: Fulmaka, Aviwe
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/54598 , vital:26592
- Description: Portulacaria afra commonly known as Spekboom contributes significantly to carbon sequestration and has been widely planted in degraded areas of the Eastern Cape. Approximately 50% of planted cuttings do not survive although the cause of this decline is unknown. Like many indigenous plants, Spekboom forms a symbiotic relationship with mycorrhizal fungi and the interaction with rhizobacteria may enhance and improve plant growth and establishment. This study aims to investigate these relationships which will include a survey of the arbuscular mycorrhizal (AM) fungal populations associated with Spekboom, determination of the causal agent of Spekboom decline, isolation and identification of the associated rhizobacteria and investigation of their plant growth promotion properties and assessing the ability of arbuscular mycorrhizal fungi and selected rhizobacteria to enhance establishment and growth of Spekboom. Soil and root samples from selected trial sites were used to assess AM fungal spore abundance and colonisation; isolation, characterization, and identification of rhizobacteria and determine the interaction of the microbes on Spekboom growth and tolerance to Fusarium. AM spore abundance and percentage root colonisation did not differ between the three Spekboom plots. Molecular analyses of the SSU region from the plots showed 4 families of AM fungi and were identified as Ambisporaceae, Glomeraceae, Claroideoglomeraceae and Paraglomeraceae. A suspected Fusarium pathogen was isolated and molecularly identified. Pathogenicity tests indicated reduced Spekboom growth with poor root development. Thirty four rhizobacterial isolates were tested for various plant growth promoting abilities. Of these, 6 were able to produce IAA which may promote plant root growth, 27 siderophores and 23 were phosphate solubilisers. Bacterial isolates were molecularly identified to be from various species of Bacillus, with some Arthrobacter, Enterobacter, Pseudomonas and Microbacterium. Inoculation of Spekboom cuttings with mycorrhizal fungi and selected rhizobacterial isolates significantly improved shoot height. Spekboom cuttings challenged with Fusarium and inoculated with mycorrhizal fungi and two rhizobacterial isolates significantly improved growth. The inoculation of cuttings in the nursery with mycorrhizal fungi and selected rhizobacteria is recommended prior to establishing Spekboom in the field.
- Full Text:
- Date Issued: 2016
The effect of kelp supplementation in formulated feed on the production performance and gut microbiota of South African abalone (Haliotis midae)
- Authors: Nel, Aldi
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/899 , vital:20001
- Description: Formulated feeds with a relatively low (< 5 % of dry mass) kelp (Ecklonia maxima) inclusion level are widely used on commercial abalone (Haliotis midae) farms in South Africa. Although the use of kelp, a major constituent of the natural diet of H. midae, as a dietary supplement is considered to enhance abalone growth and feed utilisation, there are no published studies which quantify the effects of kelp inclusion in formulated feeds. Furthermore, the physiological mechanisms by which kelp supplementation may positively influence abalone digestive physiology and growth are largely unknown. As the kelp supplement is comprised mostly of soluble fibres and abalone gut bacteria associated with macroalgae (and its fibrous polysaccharides) are known to play a key role in digestion, it was hypothesised that the kelp supplement influences the gut-bacterial community profiles of cultured abalone through prebiotic and other metabolic effects. The present thesis thus examined the effect of kelp supplementation on the performance of abalone (Haliotis midae) fed formulated feeds and explored the influence of a kelp supplement on the abalone gut microbiota and its function in the gastrointestinal tract. The key hypotheses of the study were that kelp supplementation in formulated feed: 1) enhances abalone growth; 2) causes a shift in abalone gut-bacterial community composition through a prebiotic-like effect; 3) may induce changes in crop morphology as a result of potential bacterial-associated increases in volatile short-chain fatty acids, and 4) alters digestive enzyme activities in the abalone gut through changes in bacterial-derived (exogenous) digestive enzymes. The growth-promoting efficacy of low-level kelp supplementation was tested by feeding isonitrogenous and isoenergetic experimental feeds containing 0.00 – 3.54 % kelp (dry mass) to sub-adult abalone (~43 mm shell length) for eight months under commercial farm conditions. The growth trial established that kelp supplementation (0.44 – 3.54 % of dry mass) promoted faster growth and improved feed conversion and protein efficiency ratios in cultured abalone compared to abalone fed the non-supplemented control diet, while there were no significant differences in growth for abalone fed the different kelp-supplemented diets (0.44, 0.88, 1.76 and 3.54 % of dry mass). Feed conversion and protein efficiency ratios displayed significant correlations with kelp level in the range of 0.00 – 3.54 % dry mass, and it is therefore recommended that kelp be included in the formulated feeds of cultured South African abalone at a rate of up to 3.54 % of dry mass. A kelp-supplemented (0.88 % dry weight inclusion) feed was fed to abalone under farm conditions to compare gut physiological parameters (crop morphology, digestive enzyme activities and the gut microbiota) in abalone against that of abalone fed an isonitrogenous and isoenergetic non-supplemented control feed. To establish if the observed higher abalone growth rates were related to improved gastrointestinal tract epithelium activity and integrity, as reflected by epithelial cell growth in response to potential changes in bacterial-derived short-chain fatty acid production, crop epithelial morphology was compared between abalone fed the kelp-supplemented and control feeds. Kelp supplementation did not induce any observable changes in crop epithelial cell height for farm-reared sub-adult abalone fed the experimental diets on-farm for seven weeks. This was attributed to the similar macronutrient compositions of kelp-supplemented and control diets and/ or the common diet history of experimental abalone from weaning to the initiation of the experiment. Digestive enzyme activity was compared between abalone fed a kelp-supplemented and a control feed during an on-farm feeding trial with sub-adult abalone. Gut samples were collected after seven weeks and colorimetric enzyme assays were performed for the polysaccharide-degrading enzymes amylase, alginate lyase, laminarinase and fucoidanase, and for acid protease, trypsin and chymotrypsin activity. Amylase and alginate lyase activities were relatively high, compared to the other enzymes. Polysaccharidase and acid protease activity levels did not differ significantly between abalone fed kelp-supplemented and control feeds, but a greater variability in enzyme activity levels was observed in abalone fed the control diet. It was hypothesised that this might be due to the kelp supplement promoting a more stable and less opportunistic gut-bacterial community than the control diet. Pooled gut samples of abalone fed the kelp-supplemented diet were used for proteomic analyses to identify the composition of enzyme proteins of both endogenous and exogenous origin in the abalone digestive system. The key polysaccharidases and proteases in the gut samples of kelp-supplemented formulated feed-fed abalone were all of abalone origin, whereas the bacterial enzymes were of the types that form part of intermediate reactions in metabolic pathways. The results suggested that bacterial enzymes play a different role to abalone endogenous enzymes in the digestion of formulated feed. While abalone enzymes appear to be the main degraders of carbohydrate and protein macromolecules, the profile of exogenous enzymes suggests that they perform bioconversions of smaller organic compounds. The profiles of gut-bacterial communities of farm-reared sub-adult abalone fed kelpsupplemented and control feeds on-farm for seven weeks were analysed with metagenomic pyrosequencing and DGGE analyses, using 16S rDNA-targeted amplified DNA. The results indicated a shift in gut-bacterial composition with a higher abundance of Mollicutes in abalone fed kelp-supplemented feed compared to those fed the control feed. DGGE band patterns displayed a greater within-group similarity in gut bacteria for abalone fed the kelpsupplemented diet and the presence of unique and variable bands for bacteria in the guts of abalone fed the control diet. It was concluded that when cultured abalone are fed kelpsupplemented formulated feeds, more stable gut bacterial communities are present compared to a more opportunistic gut-bacterial community in abalone fed non-supplemented feeds, and that the observed increase in Mollicutes could reflect the restoration of the abalone gut microbiota to a more natural state. The novel application of proteomics to abalone nutrition in the present study demonstrated that gut-bacterial enzymes may form part of many different metabolic pathways and suggests that the metabolism of the gut microbiota serves as an extension of the abalone’s digestive metabolism. Future studies should quantify the contribution of commensal gut-bacteria to cultured abalone nutrition by employing metabolomic studies to characterize the utilisation of bacterial-derived metabolites by the abalone host.
- Full Text:
- Date Issued: 2016
- Authors: Nel, Aldi
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/899 , vital:20001
- Description: Formulated feeds with a relatively low (< 5 % of dry mass) kelp (Ecklonia maxima) inclusion level are widely used on commercial abalone (Haliotis midae) farms in South Africa. Although the use of kelp, a major constituent of the natural diet of H. midae, as a dietary supplement is considered to enhance abalone growth and feed utilisation, there are no published studies which quantify the effects of kelp inclusion in formulated feeds. Furthermore, the physiological mechanisms by which kelp supplementation may positively influence abalone digestive physiology and growth are largely unknown. As the kelp supplement is comprised mostly of soluble fibres and abalone gut bacteria associated with macroalgae (and its fibrous polysaccharides) are known to play a key role in digestion, it was hypothesised that the kelp supplement influences the gut-bacterial community profiles of cultured abalone through prebiotic and other metabolic effects. The present thesis thus examined the effect of kelp supplementation on the performance of abalone (Haliotis midae) fed formulated feeds and explored the influence of a kelp supplement on the abalone gut microbiota and its function in the gastrointestinal tract. The key hypotheses of the study were that kelp supplementation in formulated feed: 1) enhances abalone growth; 2) causes a shift in abalone gut-bacterial community composition through a prebiotic-like effect; 3) may induce changes in crop morphology as a result of potential bacterial-associated increases in volatile short-chain fatty acids, and 4) alters digestive enzyme activities in the abalone gut through changes in bacterial-derived (exogenous) digestive enzymes. The growth-promoting efficacy of low-level kelp supplementation was tested by feeding isonitrogenous and isoenergetic experimental feeds containing 0.00 – 3.54 % kelp (dry mass) to sub-adult abalone (~43 mm shell length) for eight months under commercial farm conditions. The growth trial established that kelp supplementation (0.44 – 3.54 % of dry mass) promoted faster growth and improved feed conversion and protein efficiency ratios in cultured abalone compared to abalone fed the non-supplemented control diet, while there were no significant differences in growth for abalone fed the different kelp-supplemented diets (0.44, 0.88, 1.76 and 3.54 % of dry mass). Feed conversion and protein efficiency ratios displayed significant correlations with kelp level in the range of 0.00 – 3.54 % dry mass, and it is therefore recommended that kelp be included in the formulated feeds of cultured South African abalone at a rate of up to 3.54 % of dry mass. A kelp-supplemented (0.88 % dry weight inclusion) feed was fed to abalone under farm conditions to compare gut physiological parameters (crop morphology, digestive enzyme activities and the gut microbiota) in abalone against that of abalone fed an isonitrogenous and isoenergetic non-supplemented control feed. To establish if the observed higher abalone growth rates were related to improved gastrointestinal tract epithelium activity and integrity, as reflected by epithelial cell growth in response to potential changes in bacterial-derived short-chain fatty acid production, crop epithelial morphology was compared between abalone fed the kelp-supplemented and control feeds. Kelp supplementation did not induce any observable changes in crop epithelial cell height for farm-reared sub-adult abalone fed the experimental diets on-farm for seven weeks. This was attributed to the similar macronutrient compositions of kelp-supplemented and control diets and/ or the common diet history of experimental abalone from weaning to the initiation of the experiment. Digestive enzyme activity was compared between abalone fed a kelp-supplemented and a control feed during an on-farm feeding trial with sub-adult abalone. Gut samples were collected after seven weeks and colorimetric enzyme assays were performed for the polysaccharide-degrading enzymes amylase, alginate lyase, laminarinase and fucoidanase, and for acid protease, trypsin and chymotrypsin activity. Amylase and alginate lyase activities were relatively high, compared to the other enzymes. Polysaccharidase and acid protease activity levels did not differ significantly between abalone fed kelp-supplemented and control feeds, but a greater variability in enzyme activity levels was observed in abalone fed the control diet. It was hypothesised that this might be due to the kelp supplement promoting a more stable and less opportunistic gut-bacterial community than the control diet. Pooled gut samples of abalone fed the kelp-supplemented diet were used for proteomic analyses to identify the composition of enzyme proteins of both endogenous and exogenous origin in the abalone digestive system. The key polysaccharidases and proteases in the gut samples of kelp-supplemented formulated feed-fed abalone were all of abalone origin, whereas the bacterial enzymes were of the types that form part of intermediate reactions in metabolic pathways. The results suggested that bacterial enzymes play a different role to abalone endogenous enzymes in the digestion of formulated feed. While abalone enzymes appear to be the main degraders of carbohydrate and protein macromolecules, the profile of exogenous enzymes suggests that they perform bioconversions of smaller organic compounds. The profiles of gut-bacterial communities of farm-reared sub-adult abalone fed kelpsupplemented and control feeds on-farm for seven weeks were analysed with metagenomic pyrosequencing and DGGE analyses, using 16S rDNA-targeted amplified DNA. The results indicated a shift in gut-bacterial composition with a higher abundance of Mollicutes in abalone fed kelp-supplemented feed compared to those fed the control feed. DGGE band patterns displayed a greater within-group similarity in gut bacteria for abalone fed the kelpsupplemented diet and the presence of unique and variable bands for bacteria in the guts of abalone fed the control diet. It was concluded that when cultured abalone are fed kelpsupplemented formulated feeds, more stable gut bacterial communities are present compared to a more opportunistic gut-bacterial community in abalone fed non-supplemented feeds, and that the observed increase in Mollicutes could reflect the restoration of the abalone gut microbiota to a more natural state. The novel application of proteomics to abalone nutrition in the present study demonstrated that gut-bacterial enzymes may form part of many different metabolic pathways and suggests that the metabolism of the gut microbiota serves as an extension of the abalone’s digestive metabolism. Future studies should quantify the contribution of commensal gut-bacteria to cultured abalone nutrition by employing metabolomic studies to characterize the utilisation of bacterial-derived metabolites by the abalone host.
- Full Text:
- Date Issued: 2016
Synthesis, spectroscopic and nonlinear optical properties of metal-free and nickel β substituted binuclear phthalocyanines
- Authors: Kabwe, Kapambwe Peter
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3013 , vital:20352
- Description: In this project, nickel and metal-free 4β-(4-tert-butylphenoxy) phthalocyanine, biphenyl bridged binuclear 4-tert-butylphenoxy phthalocyanine and naphthalene bridged binuclear 4-tert-butylphenoxy phthalocyanine have been synthesised. The Z-scan technique has been employed to comparatively study their second order nonlinear optical (NLO) properties. This work, shows that the presence of H-aggregation in binuclear Pcs of metal-free and nickel 4β-(4-tertbutylphenoxy) phthalocyanines do not have an effect on the magnitude of second order nonlinear absorption coefficient (β) as compared to monomeric Pcs. Density functional (DFT) calculations of dipolar/octupolar contributions were performed, in order to explain experimentally determined β values. Spectroscopic and photophysical properties of the synthesised compounds have been determined using a range of different spectroscopic techniques, including magnetic circular dichroism (MCD), time correlated single photon counting spectroscopy (TCSPC), UV-visible absorption spectroscopy, mass spectroscopy and IR Spectroscopy.
- Full Text:
- Date Issued: 2016
- Authors: Kabwe, Kapambwe Peter
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3013 , vital:20352
- Description: In this project, nickel and metal-free 4β-(4-tert-butylphenoxy) phthalocyanine, biphenyl bridged binuclear 4-tert-butylphenoxy phthalocyanine and naphthalene bridged binuclear 4-tert-butylphenoxy phthalocyanine have been synthesised. The Z-scan technique has been employed to comparatively study their second order nonlinear optical (NLO) properties. This work, shows that the presence of H-aggregation in binuclear Pcs of metal-free and nickel 4β-(4-tertbutylphenoxy) phthalocyanines do not have an effect on the magnitude of second order nonlinear absorption coefficient (β) as compared to monomeric Pcs. Density functional (DFT) calculations of dipolar/octupolar contributions were performed, in order to explain experimentally determined β values. Spectroscopic and photophysical properties of the synthesised compounds have been determined using a range of different spectroscopic techniques, including magnetic circular dichroism (MCD), time correlated single photon counting spectroscopy (TCSPC), UV-visible absorption spectroscopy, mass spectroscopy and IR Spectroscopy.
- Full Text:
- Date Issued: 2016
Views from the inside: An appraisal of the effectiveness of international NGOs as agents of development through a case study of Concern Universal’s Local Development Support Programme in Dedza District, Malawi
- Authors: Mussa, Khadija Sungeni
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/1499 , vital:20063
- Description: Malawi, which became independent in 1964, attracted Non-Governmental Organisations (NGOs) from the early 1980s. Initially, NGO involvement was a response to the influx of refugees from neighbouring war-torn Mozambique. Since then, NGOs have been active in the development sector. Malawi, a small country, has widespread poverty, and has recently been in international headlines as a victim of floods, drought and food shortages. Economically unstable, with environmental problems, Malawi is in need of development assistance. NGOs have been centrally positioned in such efforts, but the academic literature on their role has been limited. NGO interventions in development efforts, generally, have been subject to controversy. While some argue that NGOs provide an essential means of development, especially where state capacity is limited, others argue that, with most NGOs headquartered or funded from abroad, their strategies and practices are often more accountable to external pressures than local needs. This thesis intervenes in these debates with a case study: with the aim of examining the sustainability, appropriateness, accountability and effectiveness of NGO projects, it looks at a project by the international NGO (INGO), Concern Universal (CU), which works in the central region in Dedza, Malawi. It examines the project, using fieldwork in three villages, looking at issues such as its use of participatory methods, relations with local government and village structures, capacity building methods, and donor relations. The thesis argues that (I)NGOs like CU exist in a conflicted situation: they have to remain in the good books of their donors, while, at the same time, maintaining accountability to their beneficiaries; they depend on their ability to manoeuvre through the conflict in order to ensure their continuity, and so, their impact is shaped by competing imperatives. CU has made a real impact on poverty alleviation efforts, but its methods and approaches are shaped by said competing imperatives.
- Full Text:
- Date Issued: 2016
- Authors: Mussa, Khadija Sungeni
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/1499 , vital:20063
- Description: Malawi, which became independent in 1964, attracted Non-Governmental Organisations (NGOs) from the early 1980s. Initially, NGO involvement was a response to the influx of refugees from neighbouring war-torn Mozambique. Since then, NGOs have been active in the development sector. Malawi, a small country, has widespread poverty, and has recently been in international headlines as a victim of floods, drought and food shortages. Economically unstable, with environmental problems, Malawi is in need of development assistance. NGOs have been centrally positioned in such efforts, but the academic literature on their role has been limited. NGO interventions in development efforts, generally, have been subject to controversy. While some argue that NGOs provide an essential means of development, especially where state capacity is limited, others argue that, with most NGOs headquartered or funded from abroad, their strategies and practices are often more accountable to external pressures than local needs. This thesis intervenes in these debates with a case study: with the aim of examining the sustainability, appropriateness, accountability and effectiveness of NGO projects, it looks at a project by the international NGO (INGO), Concern Universal (CU), which works in the central region in Dedza, Malawi. It examines the project, using fieldwork in three villages, looking at issues such as its use of participatory methods, relations with local government and village structures, capacity building methods, and donor relations. The thesis argues that (I)NGOs like CU exist in a conflicted situation: they have to remain in the good books of their donors, while, at the same time, maintaining accountability to their beneficiaries; they depend on their ability to manoeuvre through the conflict in order to ensure their continuity, and so, their impact is shaped by competing imperatives. CU has made a real impact on poverty alleviation efforts, but its methods and approaches are shaped by said competing imperatives.
- Full Text:
- Date Issued: 2016
Gold nanoparticle–based colorimetric probes for dopamine detection
- Authors: Ngomane, Nokuthula
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4565 , http://hdl.handle.net/10962/d1021261
- Description: Colorimetric probes have become important tools in analysis and biomedical technology. This thesis reports on the development of such probes for the detection of dopamine (DA). Liquid and different solid state probes were developed utilizing un–functionalized gold nanoparticles (UF–AuNPs). The liquid state probe is based on the growth and aggregation of the UF–AuNPs in the presence of DA. Upon addition of the UF–AuNPs to various concentrations of DA, the shape, size and colour change results in spectral shifts from lower to higher wavelengths. The analyte can be easily monitored by the naked eye from 5.0 nM DA with a calculated limit of detection of 2.5 nM (3σ) under optimal pH. Ascorbic acid (AA) has a potential to interfere with DA detection in solution since it is often present in biological fluids, but in this case the interference was limited to solutions where its concentration was beyond 200 times greater than that of DA. Since most of the previously reported colorimetric probes, especially those for DA are solution based, the main focus of the thesis was in the development of a solid state based colorimetric probe in the form of nanofibre mats. To overcome the interference challenges experienced in the solution studies (the interference by high concentrations of AA), the suitability of molecularly imprinted polymers (MIPs) for the selective detection of DA was investigated. The results showed that the MIPs produced did not play a significant role in enhancing the selectivity towards DA. A probe composed of just the UF–AuNPs and Nylon–6 (UF–AuNPs + N6) was also developed. The UF–AuNPs were synthesized following an in situ reduction method. The probe was only selective to DA and insensitive to other catecholamines at physiological pH. Thus, the probe did not require any addition functionalities to achieve selectivity and sensitive to DA. The liquid state probe and the composite UF–AuNPs + N6 nanofibre probe were successfully applied to a whole blood sample and showed good selectivity towards DA. The simple, sensitive and selective probe could be an excellent alternative for on–site and immediate detection of DA without the use of instrumentation. For quantification of DA using the solid state probe, open–source software imageJ was used to assist in the analysis of the nanofibre colours. It was observed that the intensity of the colour increased with the increase in concentration of DA in a linear fashion. The use of imageJ can also be a great alternative where the colour changes are not so clear or for visually impaired people. The solid state probe developed can detect DA qualitatively and quantitatively. The work also forms a good foundation for development of such probes for other analyte.
- Full Text:
- Date Issued: 2016
- Authors: Ngomane, Nokuthula
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4565 , http://hdl.handle.net/10962/d1021261
- Description: Colorimetric probes have become important tools in analysis and biomedical technology. This thesis reports on the development of such probes for the detection of dopamine (DA). Liquid and different solid state probes were developed utilizing un–functionalized gold nanoparticles (UF–AuNPs). The liquid state probe is based on the growth and aggregation of the UF–AuNPs in the presence of DA. Upon addition of the UF–AuNPs to various concentrations of DA, the shape, size and colour change results in spectral shifts from lower to higher wavelengths. The analyte can be easily monitored by the naked eye from 5.0 nM DA with a calculated limit of detection of 2.5 nM (3σ) under optimal pH. Ascorbic acid (AA) has a potential to interfere with DA detection in solution since it is often present in biological fluids, but in this case the interference was limited to solutions where its concentration was beyond 200 times greater than that of DA. Since most of the previously reported colorimetric probes, especially those for DA are solution based, the main focus of the thesis was in the development of a solid state based colorimetric probe in the form of nanofibre mats. To overcome the interference challenges experienced in the solution studies (the interference by high concentrations of AA), the suitability of molecularly imprinted polymers (MIPs) for the selective detection of DA was investigated. The results showed that the MIPs produced did not play a significant role in enhancing the selectivity towards DA. A probe composed of just the UF–AuNPs and Nylon–6 (UF–AuNPs + N6) was also developed. The UF–AuNPs were synthesized following an in situ reduction method. The probe was only selective to DA and insensitive to other catecholamines at physiological pH. Thus, the probe did not require any addition functionalities to achieve selectivity and sensitive to DA. The liquid state probe and the composite UF–AuNPs + N6 nanofibre probe were successfully applied to a whole blood sample and showed good selectivity towards DA. The simple, sensitive and selective probe could be an excellent alternative for on–site and immediate detection of DA without the use of instrumentation. For quantification of DA using the solid state probe, open–source software imageJ was used to assist in the analysis of the nanofibre colours. It was observed that the intensity of the colour increased with the increase in concentration of DA in a linear fashion. The use of imageJ can also be a great alternative where the colour changes are not so clear or for visually impaired people. The solid state probe developed can detect DA qualitatively and quantitatively. The work also forms a good foundation for development of such probes for other analyte.
- Full Text:
- Date Issued: 2016
Exploring functionings and conversion factors in biodiversity teacher professional learning communities
- Tshiningayamwe, Sirkka Alina Nambashun
- Authors: Tshiningayamwe, Sirkka Alina Nambashun
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2080 , http://hdl.handle.net/10962/d1021313
- Description: The study explores the conversion factors, functionings (valued beings and doings), agency and structures in Professional Learning Communities (PLCs) for Life Sciences teachers’ biodiversity knowledge. The teachers’ valued beings and doings as well as conversion factors associated with these beings and doings were discussed within the conceptual framework of the capability approach using three PLCs in South Africa. Two PLCs were in the Eastern Cape Province (Grahamstown and Idutywa district), and one PLC was in the Western Cape (Cape Town) province. The PLCs involved in this study were course initiated and were positioned in the Fundisa for Change national teacher education programme. Fundisa for Change is a partnership programme that aims to enhance transformative environmental learning through teacher education. To illuminate constrained capabilities and how and to what extent the Life Sciences teachers’ empirical actions are related to these, the concepts of the capability approach were underlaboured with critical realism’s causal view of human action. A critical realist theory of causation was useful in explaining how the teachers’ valued beings and doings, conversion factors and capability sets can be partly accounted for via an understanding of underlying mechanisms that are generative of events and empirical experience. The study used a qualitative case study research methodology. Interviews, questionnaires, observations (of PLC activities), document reviews (of teachers’ portfolios of evidence, Fundisa for Change implementation plan, evaluation forms and resources materials, and policy documents) and reflection tools were used to collect data. Using the critical realism modes of inference (induction, abduction and retroduction), the data was analysed in two phases. Phase one analysis was primarily inductive and used thick descriptions (mainly in the form of quotes) to present and discuss the teachers’ valued beings and doings and associated conversion factors in the PLCs. This phase of analysis was abductive. The study reported four main functionings valued by teachers: subject content knowledge, teaching practices, assessment practices, and use of teaching and learning support materials. These valued functionings were discussed in light of the beings and doings in the PLCs and the underlying mechanisms related to teachers’ biodiversity teaching. Conversion factors that were associated with the teachers’ valued beings and doings in the PLCs were discussed in line with capability approach’s environmental, social and personal conversion factors. The study found that most of the conversion factors within the PLCs and the Fundisa for Change professional development programme (good facilitation, collaborative learning space, site where PLC activities happened, individual teachers’ capabilities, teaching and learning support materials and policy documents) were enablers to the teachers’ capabilities for biodiversity teaching, and thus enhanced teachers’ knowledge for biodiversity teaching. The study further found that teachers realised some of their achieved functionings in their actual teaching of biodiversity content in the Life Sciences curriculum, and that factors such as lack of resources, large class sizes, learners’ abilities and lack of interest among some teachers were amongst the factors that constrained teachers’ realisation of their achieved functionings in the PLCs. The study therefore revealed that if professional development programmes take account of underlying mechanisms and respond to teachers’ capabilities i.e. their valued functionings for biodiversity teaching in the Life Sciences curriculum, the professional development programmes can be an important conversion factor that enables the expansion of teachers’ capabilities (especially their biodiversity knowledge, pedagogical and assessment practice but also other capabilities) in ways that have the potential to reshape teachers’ classroom practices related to the teaching of biodiversity.
- Full Text:
- Date Issued: 2016
- Authors: Tshiningayamwe, Sirkka Alina Nambashun
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2080 , http://hdl.handle.net/10962/d1021313
- Description: The study explores the conversion factors, functionings (valued beings and doings), agency and structures in Professional Learning Communities (PLCs) for Life Sciences teachers’ biodiversity knowledge. The teachers’ valued beings and doings as well as conversion factors associated with these beings and doings were discussed within the conceptual framework of the capability approach using three PLCs in South Africa. Two PLCs were in the Eastern Cape Province (Grahamstown and Idutywa district), and one PLC was in the Western Cape (Cape Town) province. The PLCs involved in this study were course initiated and were positioned in the Fundisa for Change national teacher education programme. Fundisa for Change is a partnership programme that aims to enhance transformative environmental learning through teacher education. To illuminate constrained capabilities and how and to what extent the Life Sciences teachers’ empirical actions are related to these, the concepts of the capability approach were underlaboured with critical realism’s causal view of human action. A critical realist theory of causation was useful in explaining how the teachers’ valued beings and doings, conversion factors and capability sets can be partly accounted for via an understanding of underlying mechanisms that are generative of events and empirical experience. The study used a qualitative case study research methodology. Interviews, questionnaires, observations (of PLC activities), document reviews (of teachers’ portfolios of evidence, Fundisa for Change implementation plan, evaluation forms and resources materials, and policy documents) and reflection tools were used to collect data. Using the critical realism modes of inference (induction, abduction and retroduction), the data was analysed in two phases. Phase one analysis was primarily inductive and used thick descriptions (mainly in the form of quotes) to present and discuss the teachers’ valued beings and doings and associated conversion factors in the PLCs. This phase of analysis was abductive. The study reported four main functionings valued by teachers: subject content knowledge, teaching practices, assessment practices, and use of teaching and learning support materials. These valued functionings were discussed in light of the beings and doings in the PLCs and the underlying mechanisms related to teachers’ biodiversity teaching. Conversion factors that were associated with the teachers’ valued beings and doings in the PLCs were discussed in line with capability approach’s environmental, social and personal conversion factors. The study found that most of the conversion factors within the PLCs and the Fundisa for Change professional development programme (good facilitation, collaborative learning space, site where PLC activities happened, individual teachers’ capabilities, teaching and learning support materials and policy documents) were enablers to the teachers’ capabilities for biodiversity teaching, and thus enhanced teachers’ knowledge for biodiversity teaching. The study further found that teachers realised some of their achieved functionings in their actual teaching of biodiversity content in the Life Sciences curriculum, and that factors such as lack of resources, large class sizes, learners’ abilities and lack of interest among some teachers were amongst the factors that constrained teachers’ realisation of their achieved functionings in the PLCs. The study therefore revealed that if professional development programmes take account of underlying mechanisms and respond to teachers’ capabilities i.e. their valued functionings for biodiversity teaching in the Life Sciences curriculum, the professional development programmes can be an important conversion factor that enables the expansion of teachers’ capabilities (especially their biodiversity knowledge, pedagogical and assessment practice but also other capabilities) in ways that have the potential to reshape teachers’ classroom practices related to the teaching of biodiversity.
- Full Text:
- Date Issued: 2016
Identification of potential novel roles for Hsp70/Hsp90 organising protein (Hop) using proteomic analysis in human cells
- Authors: Wingate, Ianthe
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/64758 , vital:28598
- Description: Expected release date-May 2018
- Full Text:
- Date Issued: 2016
- Authors: Wingate, Ianthe
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/64758 , vital:28598
- Description: Expected release date-May 2018
- Full Text:
- Date Issued: 2016
Selecting and augmenting a FOSS development and deployment environment for personalized video-oriented services in a Telco context
- Authors: Shibeshi, Zelalem Sintayehu
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/943 , vital:20005
- Description: The great demand for video services on the Internet is one contributing factor that led telecom companies to search for solutions to deliver innovative video services, using the different access technologies managed by them and leveraging the capacity of enforcing Quality of Service (QoS). One part of the solution was an infrastructure that guarantees QoS for these services, in the form of the IP Multimedia Subsystem (IMS) framework. The IMS framework was developed for delivering innovative multimedia services, but IMS alone does not provide the required services. This has led to further work in the area of multimedia service architectures. One noteworthy architecture is IPTV. IPTV is more than what its name implies, as it allows the development of various innovative video-oriented services and not just tv. When IPTV was introduced, many thought that it would bring back the revenue loss that telecom companies experienced to over-the-top (OTT) service providers. However, despite all its promises, the IPTV implementation has not shown as wide an uptake as one would expect. Although there could be various reasons for the slow penetration of IPTV, one reason could be the technical challenge that IPTV poses to service developers. One of the main reasons for the embarking of the research reported in this thesis was to identify and select free and open source software (FOSS) based platforms and augment them for easy development and deployment of video-oriented services. The thesis motivated how the IPTV architecture, with some modification, can be a good architecture to develop innovative video-oriented services. For a better understanding and investigate the issues of video-oriented service development on different platforms, we followed an incremental and iterative prototyping method. As a result, various video-oriented services were first developed and implementation-related issues were analyzed. This has helped us to identify problems that service developers face, including the requirement to utilize a number of protocols to develop an IPTV-based video-oriented service and the lack of a platform that provides a consistent programming interface to implement them all. The process also helped us to identify new uses cases through the process. As part of our selection process, we found that the Mobicents service development platform can be used as the basis for a good service development and deployment environment for video-oriented services. Mobicents is a Java-based service delivery platform for quick development, deployment and management of next generation network applications. Mobicents is a good choice because it provides a consistent programming interface and supports the various protocols needed in a consistent manner or an easy way to include the support for them. We used Mobicents to compose the environment that developers can use to build video-oriented services. Specifically we developed components and service building blocks that service developer can use to develop various innovative video-oriented services. During our research, we also identified various issues with regard to support from streaming servers in general and open source streaming servers in particular and also with the protocol they use. Specifically, we identified issues with Real Time Streaming Protocol (RTSP), a protocol specified as the media control protocol in the IPTV specification, and made proposals for solving them. We developed an RSTP proxy to augment the features lacking in the current streaming servers and implemented some of the features we proposed in it.
- Full Text:
- Date Issued: 2016
- Authors: Shibeshi, Zelalem Sintayehu
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/943 , vital:20005
- Description: The great demand for video services on the Internet is one contributing factor that led telecom companies to search for solutions to deliver innovative video services, using the different access technologies managed by them and leveraging the capacity of enforcing Quality of Service (QoS). One part of the solution was an infrastructure that guarantees QoS for these services, in the form of the IP Multimedia Subsystem (IMS) framework. The IMS framework was developed for delivering innovative multimedia services, but IMS alone does not provide the required services. This has led to further work in the area of multimedia service architectures. One noteworthy architecture is IPTV. IPTV is more than what its name implies, as it allows the development of various innovative video-oriented services and not just tv. When IPTV was introduced, many thought that it would bring back the revenue loss that telecom companies experienced to over-the-top (OTT) service providers. However, despite all its promises, the IPTV implementation has not shown as wide an uptake as one would expect. Although there could be various reasons for the slow penetration of IPTV, one reason could be the technical challenge that IPTV poses to service developers. One of the main reasons for the embarking of the research reported in this thesis was to identify and select free and open source software (FOSS) based platforms and augment them for easy development and deployment of video-oriented services. The thesis motivated how the IPTV architecture, with some modification, can be a good architecture to develop innovative video-oriented services. For a better understanding and investigate the issues of video-oriented service development on different platforms, we followed an incremental and iterative prototyping method. As a result, various video-oriented services were first developed and implementation-related issues were analyzed. This has helped us to identify problems that service developers face, including the requirement to utilize a number of protocols to develop an IPTV-based video-oriented service and the lack of a platform that provides a consistent programming interface to implement them all. The process also helped us to identify new uses cases through the process. As part of our selection process, we found that the Mobicents service development platform can be used as the basis for a good service development and deployment environment for video-oriented services. Mobicents is a Java-based service delivery platform for quick development, deployment and management of next generation network applications. Mobicents is a good choice because it provides a consistent programming interface and supports the various protocols needed in a consistent manner or an easy way to include the support for them. We used Mobicents to compose the environment that developers can use to build video-oriented services. Specifically we developed components and service building blocks that service developer can use to develop various innovative video-oriented services. During our research, we also identified various issues with regard to support from streaming servers in general and open source streaming servers in particular and also with the protocol they use. Specifically, we identified issues with Real Time Streaming Protocol (RTSP), a protocol specified as the media control protocol in the IPTV specification, and made proposals for solving them. We developed an RSTP proxy to augment the features lacking in the current streaming servers and implemented some of the features we proposed in it.
- Full Text:
- Date Issued: 2016
Exploring the role of corrective feedback in helping Grade 8 learners to improve the accuracy of their written English: an action research case study
- Authors: Miranda, Zoachina Nangobe
- Date: 2016
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2072 , http://hdl.handle.net/10962/d1021167
- Description: This action research study explored and analysed the role of teacher corrective feedback in helping Grade 8 learners to improve the accuracy of their written English as their second language. Therefore, the goals of this study were to examine the kind of language errors my grade 8 learners’ made in their writing, to find out whether these errors could be categorized linguistically, and to determine if they were errors, mistakes or lapses. The study further analysed how learners responded to my feedback, and also determined which feedback strategies worked best to help my learners deal with their errors, mistakes or lapses. This study set out to look at six learners from one Grade 8 class of 40 learners. The data were gathered from six written essay scripts, and each learner wrote four essay draft revisions. The learners’ written essays were analysed by means of checklists in order to identify the types and patterns of errors made. Errors such as punctuation, past tense verbs, spelling and vocabulary were identified, analysed and categorized to provide insights into reasons underlying the instances in which they were committed. The findings of this study showed that factors underlying learners’ written errors included mother-tongue interference, overgeneralization, fossilization, translation, lack of concentration, and carelessness. The findings further showed that corrective feedback on learners’ draft revisions provided them with extensive exposure and practice in English, enabled them to internalize language rules, and reduced the tendency to commit errors in their writing. The findings further suggest that procedures such as multiple-draft activities, indirect feedback, direct feedback, focused corrective feedback, error correction and written feedback with explicit corrective comments improved their levels of writing. Furthermore, putting these procedures into practice and reflecting critically on how to apply them helped enrich my own teaching practices and development in relation to the provision of corrective feedback to improve accuracy in learners’ writing. The findings are discussed in the context of the related literature. This study should be read by ESL teacher-trainers, ESL teachers, ESL student-teachers and ESL learners/students in general.
- Full Text:
- Date Issued: 2016
- Authors: Miranda, Zoachina Nangobe
- Date: 2016
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2072 , http://hdl.handle.net/10962/d1021167
- Description: This action research study explored and analysed the role of teacher corrective feedback in helping Grade 8 learners to improve the accuracy of their written English as their second language. Therefore, the goals of this study were to examine the kind of language errors my grade 8 learners’ made in their writing, to find out whether these errors could be categorized linguistically, and to determine if they were errors, mistakes or lapses. The study further analysed how learners responded to my feedback, and also determined which feedback strategies worked best to help my learners deal with their errors, mistakes or lapses. This study set out to look at six learners from one Grade 8 class of 40 learners. The data were gathered from six written essay scripts, and each learner wrote four essay draft revisions. The learners’ written essays were analysed by means of checklists in order to identify the types and patterns of errors made. Errors such as punctuation, past tense verbs, spelling and vocabulary were identified, analysed and categorized to provide insights into reasons underlying the instances in which they were committed. The findings of this study showed that factors underlying learners’ written errors included mother-tongue interference, overgeneralization, fossilization, translation, lack of concentration, and carelessness. The findings further showed that corrective feedback on learners’ draft revisions provided them with extensive exposure and practice in English, enabled them to internalize language rules, and reduced the tendency to commit errors in their writing. The findings further suggest that procedures such as multiple-draft activities, indirect feedback, direct feedback, focused corrective feedback, error correction and written feedback with explicit corrective comments improved their levels of writing. Furthermore, putting these procedures into practice and reflecting critically on how to apply them helped enrich my own teaching practices and development in relation to the provision of corrective feedback to improve accuracy in learners’ writing. The findings are discussed in the context of the related literature. This study should be read by ESL teacher-trainers, ESL teachers, ESL student-teachers and ESL learners/students in general.
- Full Text:
- Date Issued: 2016