Enhancing the effectiveness of the Thaumatotibia Leucotreta (Lepidoptera: Tortricidae) sterile insect technique release programme
- Authors: Githae, Michael Mathenge
- Date: 2025-04-03
- Subjects: False codling moth , Cryptophlebia leucotreta , Insect sterilization , Pests Integrated control , Insect populations , Parasitism , Fruit Diseases and pests
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479897 , vital:78377 , DOI 10.21504/10962/479897
- Description: The false codling moth, Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae), is a key phytosanitary pest in the South African citrus industry. Due to its cryptic nature and its endemic presence in sub-Saharan Africa with a wide array of host plants, and eradication is not possible. However, the sterile insect technique (SIT) has been incorporated into an Area-Wide Integrated Pest Management (AW-IPM) programme to induce widespread population suppression. The successful implementation of the SIT programme required a series of well-researched phases, with one key phase being the determination of an effective overflooding ratio, previously established at 10:1. Despite this, various studies of T. leucotreta SIT programmes have reported higher release ratios than 10:1 in orchards, necessitating an investigation into their efficacy. This thesis aimed to understand the effects of higher release ratios, different combinations of sterile and fertile T. leucotreta of both sexes, compatibility of SIT and the egg parasitoid Trichogrammatoidea cryptophlebiae, and the pre-release mating levels during production and release stages to improve the effectiveness of the T. leucotreta SIT programme. This study initially investigated the impact of different ratios of sterile and fertile adults on fruit damage, sterile male competitiveness, and population growth in laboratory cages using Washington Navel oranges. Sterilised to fertile T. leucotreta adults at ratios of 0:1 (control), 10:1, 20:1, 40:1, and 60:1 were placed inside insect-rearing cages and allowed to mate, oviposit and infest the fruit. Treatment cages receiving sterile T. leucotreta produced significantly fewer damaged fruit, larval entries, and F1 adults compared to the control. The number of damaged fruit, larval entries, and F1 adults negatively correlated with the increase in the overflooding ratio of sterile to fertile T. leucotreta. Control cages had significantly higher fecundity and fertility compared to treatment cages. The 60:1 ratio exhibited the lowest per generation rate of increase (<1× from the parental [P1] to the F1 generation) compared to the 10:1 ratio (current release ratio). The effects of different combinations of both treated (T) and untreated (U) male (M) and female (F) adult T. leucotreta: UM×UF (control), TM×UF, UM×TF, TM×TF, and UM×UF×TM×TF on fruit damage, mating competitiveness, and per-generation rate of increase were tested. The treatments were housed in insect-rearing cages containing Navel oranges and allowed to mate, oviposit, and infest the fruit. Treatment cages with both treated male and female T. leucotreta had significantly fewer damaged fruit, larval entries, and emerged F1 adults compared to the control cages, except for the UM×UF×TM×TF combination. Similarly, control cages and UM×UF×TM×TF treatments had significantly Higher fecundity and fertility compared to other treatments. The TM×UF combination exhibited the lowest rate of increase per generation (<0.57× from the parental [P1] to F1 generation). A field cage study was conducted to evaluate the effects of various overflooding ratios and different combinations of sterile and fertile male and female T. leucotreta. However, the results were limited, and inconclusive due to collection of insufficient data, as the fruit infestation level was low. This could be attributed to the low quality of the moths released or effects of environmental variables on the moths. A laboratory study explored the susceptibility of T. leucotreta eggs resulting from various pairings of sterile and fertile moths to parasitism by Trichogrammatoidea cryptophlebiae. The ratios of sterile to fertile T. leucotreta used were: 0:1, 10:1, 20:1, 40:1, and 60:1. The resulting eggs were then exposed to T. cryptophlebiae for parasitism, and the parasitism rates of newly laid (24 h), 48 h and 72 h old eggs were evaluated. Overall, eggs from all ratios were suitable for T. cryptophlebiae development and acceptable for oviposition. Significantly higher number of parasitised eggs were recorded between the control (0:1) and ratios 40:1 and 60:1 at 48 h old eggs. Additionally, a higher proportion of flying T. cryptophlebiae emerged across the ratios, with a higher proportion of female-to-male sex ratio. Pre-release mating levels were studied during the production and release stages, divided into three stages: moth eclosion, irradiation, and release. A significantly higher number of spermatophores and percentages of mated female T. leucotreta were recorded at the eclosion and irradiation stages in January. Similarly, in May, a significantly higher number of spermatophores and percentages of mated female T. leucotreta were recoded post-irradiation and release stages in the Sundays River Valley region (SRV). Overall results indicated more spermatophores and percentages of mated female T. leucotreta at the irradiation and release stages in the SRV region. In conclusion, the study demonstrated that a release ratio exceeding 40:1 and different combinations of sterile and fertile T. leucotreta, especially the TM×UF combination, has a suppressive effect against T. leucotreta and integrating SIT with T. cryptophlebiae, shows potential for enhancing the effectiveness of the T. leucotreta SIT programme. Additionally, the mating competitiveness of sterile insects in dual-sex releases can be improved by controlling the level of pre-release matings. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Githae, Michael Mathenge
- Date: 2025-04-03
- Subjects: False codling moth , Cryptophlebia leucotreta , Insect sterilization , Pests Integrated control , Insect populations , Parasitism , Fruit Diseases and pests
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479897 , vital:78377 , DOI 10.21504/10962/479897
- Description: The false codling moth, Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae), is a key phytosanitary pest in the South African citrus industry. Due to its cryptic nature and its endemic presence in sub-Saharan Africa with a wide array of host plants, and eradication is not possible. However, the sterile insect technique (SIT) has been incorporated into an Area-Wide Integrated Pest Management (AW-IPM) programme to induce widespread population suppression. The successful implementation of the SIT programme required a series of well-researched phases, with one key phase being the determination of an effective overflooding ratio, previously established at 10:1. Despite this, various studies of T. leucotreta SIT programmes have reported higher release ratios than 10:1 in orchards, necessitating an investigation into their efficacy. This thesis aimed to understand the effects of higher release ratios, different combinations of sterile and fertile T. leucotreta of both sexes, compatibility of SIT and the egg parasitoid Trichogrammatoidea cryptophlebiae, and the pre-release mating levels during production and release stages to improve the effectiveness of the T. leucotreta SIT programme. This study initially investigated the impact of different ratios of sterile and fertile adults on fruit damage, sterile male competitiveness, and population growth in laboratory cages using Washington Navel oranges. Sterilised to fertile T. leucotreta adults at ratios of 0:1 (control), 10:1, 20:1, 40:1, and 60:1 were placed inside insect-rearing cages and allowed to mate, oviposit and infest the fruit. Treatment cages receiving sterile T. leucotreta produced significantly fewer damaged fruit, larval entries, and F1 adults compared to the control. The number of damaged fruit, larval entries, and F1 adults negatively correlated with the increase in the overflooding ratio of sterile to fertile T. leucotreta. Control cages had significantly higher fecundity and fertility compared to treatment cages. The 60:1 ratio exhibited the lowest per generation rate of increase (<1× from the parental [P1] to the F1 generation) compared to the 10:1 ratio (current release ratio). The effects of different combinations of both treated (T) and untreated (U) male (M) and female (F) adult T. leucotreta: UM×UF (control), TM×UF, UM×TF, TM×TF, and UM×UF×TM×TF on fruit damage, mating competitiveness, and per-generation rate of increase were tested. The treatments were housed in insect-rearing cages containing Navel oranges and allowed to mate, oviposit, and infest the fruit. Treatment cages with both treated male and female T. leucotreta had significantly fewer damaged fruit, larval entries, and emerged F1 adults compared to the control cages, except for the UM×UF×TM×TF combination. Similarly, control cages and UM×UF×TM×TF treatments had significantly Higher fecundity and fertility compared to other treatments. The TM×UF combination exhibited the lowest rate of increase per generation (<0.57× from the parental [P1] to F1 generation). A field cage study was conducted to evaluate the effects of various overflooding ratios and different combinations of sterile and fertile male and female T. leucotreta. However, the results were limited, and inconclusive due to collection of insufficient data, as the fruit infestation level was low. This could be attributed to the low quality of the moths released or effects of environmental variables on the moths. A laboratory study explored the susceptibility of T. leucotreta eggs resulting from various pairings of sterile and fertile moths to parasitism by Trichogrammatoidea cryptophlebiae. The ratios of sterile to fertile T. leucotreta used were: 0:1, 10:1, 20:1, 40:1, and 60:1. The resulting eggs were then exposed to T. cryptophlebiae for parasitism, and the parasitism rates of newly laid (24 h), 48 h and 72 h old eggs were evaluated. Overall, eggs from all ratios were suitable for T. cryptophlebiae development and acceptable for oviposition. Significantly higher number of parasitised eggs were recorded between the control (0:1) and ratios 40:1 and 60:1 at 48 h old eggs. Additionally, a higher proportion of flying T. cryptophlebiae emerged across the ratios, with a higher proportion of female-to-male sex ratio. Pre-release mating levels were studied during the production and release stages, divided into three stages: moth eclosion, irradiation, and release. A significantly higher number of spermatophores and percentages of mated female T. leucotreta were recorded at the eclosion and irradiation stages in January. Similarly, in May, a significantly higher number of spermatophores and percentages of mated female T. leucotreta were recoded post-irradiation and release stages in the Sundays River Valley region (SRV). Overall results indicated more spermatophores and percentages of mated female T. leucotreta at the irradiation and release stages in the SRV region. In conclusion, the study demonstrated that a release ratio exceeding 40:1 and different combinations of sterile and fertile T. leucotreta, especially the TM×UF combination, has a suppressive effect against T. leucotreta and integrating SIT with T. cryptophlebiae, shows potential for enhancing the effectiveness of the T. leucotreta SIT programme. Additionally, the mating competitiveness of sterile insects in dual-sex releases can be improved by controlling the level of pre-release matings. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2025
- Full Text:
- Date Issued: 2025-04-03
Evaluating social effects of arable field abandonment in communal areas of South Africa
- Authors: Pokwana, Sihle
- Date: 2025-04-03
- Subjects: Arable land , Land abandonment , Livelihood , Rural conditions , Food security South Africa , Land cover , South Africa Economic conditions
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479941 , vital:78382 , DOI 10.21504/10962/479941
- Description: The phenomenon of arable field abandonment, a complex and multifaceted aspect of land use dynamics, has become a subject of increasing significance in rural landscapes. Across diverse geographic contexts, the abandonment of once-cultivated fields is indicative of transformative shifts in agricultural practices, reflecting the interplay of numerous socio-economic, environmental, and cultural factors. The abandonment of arable fields in the former homelands of the Eastern Cape and KwaZulu-Natal poses a multifaceted challenge with significant social implications. Historically, agriculture has been central to these regions, yet recent trends show a shift from traditional field cultivation to alternative practices such as garden cultivation or complete cessation. This shift has heightened vulnerability to food insecurity, hunger, and poverty, particularly as high unemployment levels exacerbate the situation. Moreover, the intricate relationships between humans and their surrounding ecosystems is also affected. Therefore, understanding the drivers of arable field abandonment at the community or household level can reveal localised factors that differ from broader, generalised drivers identified in previous studies. Additionally, examining the diverse impacts on these communities is crucial for developing targeted interventions that address their specific needs and enhance resilience. This study investigates arable field abandonment in specific villages of Gotyibeni and Melani in the Eastern Cape and Manqorholweni and Mawane, in KwaZulu-Natal. Using the Driving forces, Pressures, State, Impacts, and Responses (DPSIR) framework as a conceptual framework uncovers the driving factors, pressures, state, impacts and responses of this phenomenon in these rural villages. The study’s main aim was to assess the social effects of arable field abandonment in the selected study areas. The main objective is to determine how livelihoods, social relationships, and living conditions within and between households have changed following the cessation of field cultivation. The study also employed several specific objectives: first, to identify trends in field cultivation within the study areas; second, to analyse the drivers contributing to arable field abandonment in the villages; third, to examine the impact of field abandonment on the elderly population over the past 50 years; and fourth, to investigate the level of youth engagement in agriculture, including how the abandonment of arable fields affects their participation and perceptions of agriculture as a livelihood. To achieve the objectives the study employed mixed research methods that combine satellite imagery analysis, household surveys, oral history interviews with elders, most of whom had stopped farming, and youth focus group discussions. Landsat 7 Satellite imagery with a 15-meter spatial resolution was sourced from the South African Space Agency (SANSA) for the four study areas. The temporal coverage spanned from 1972 to 2020, with considerations for image usability based on resolution. Unfortunately, many earlier images were deemed unusable due to low resolution, particularly affecting Gotyibeni, where clear images only became available from 2013, therefore for this village the focus was on two decades. Arable fields, residential areas, homestead gardens, grasslands, and shrublands were classified. Change detection utilised a post-classification approach, comparing land cover categories between the early and late 2000s. A change detection matrix and statistics were generated using a ha/year formula, enabling the quantification of land cover changes. Image analysis employed ArcGIS 10.8.1 software for mapping and classification, involving georeferencing, signature file creation, maximum likelihood classification, and conversion from raster to vector formats for area calculations. For the LULC change matrix, an intersection analysis in ArcGIS overlaid data from different time points, and a pivot table in Excel organised resulting data. A focus on arable land changes, particularly conversions to other land uses, was crucial, as the study is investigating the level of arable field abandonment in these areas, understanding how much arable land has been converted or remained unchanged provides a clear indication of the extent of abandonment. To understand driving forces of arable field abandonment, a semi-structured questionnaire was developed based on the Driving forces, Pressures, States, Impacts, and Responses (DPSIR) framework. The questionnaires were administered through household interviews conducted in the four study villages. Sections of the questionnaire covered household involvement in cropping, perceptions of land use change, abandonment of field cultivation, and socio-economic demographics. Sampling involved a random selection of 20% of total households in each village, with data analysis performed using Statistical Package for Social Sciences (SPSS). Chi-square tests and Kruskal-Wallis analysis were employed to assess differences between villages. A second questionnaire specifically targeting households that had abandoned field cultivation was also done. Ninety-seven interviews were conducted to evaluate economic, health, socio-cultural and environmental impacts from respondents that indicated to have ceased field cultivation. Data analysis through SPSS utilised descriptive statistics and chi-square tests to identify statistically significant differences between villages. Oral histories were collected from elderly individuals in each village from a sample size of 15 individuals who were previous cultivators of fields, selected with the assistance of a village leader. The selected elders provided qualitative insights into historical and contemporary experiential aspects of village life. The focus was on changes in village relations, the environment, generational values, and traditional farming practices in each village. Youth-focused group discussions were organised with 30 young people in the village between the ages of 18-35, covering topics such as youth goals, the importance of field cultivation, and perceptions of the future. Both the oral histories and focus group discussions conducted in the local languages, were tape-recorded, transcribed, and subjected to content analysis to identify and interpret themes and patterns. The approach involved coding sentences, grouping codes into sub-themes, and further organising them into categories and subcategories. The LULC change results showed a consistent trend of significant arable land reduction across all villages, accompanied by a notable increase in grassland cover. Manqorholweni experienced the most significant loss of these arable fields. This finding was further supported by the household interviews which revealed 100% abandonment of fields in this village. Grasslands emerged as the dominant land cover in all villages, overtaking arable fields and homestead gardens. Regarding field abandonment, 76% of households across villages left fields unused for over 19 years due to reasons including lack of fencing, animal damage to crops, and financial constraints, despite recognising the socio-economic importance of field cultivation. Respondents expressed interest in returning to cultivation if the state would assist with fencing materials and costs. Concerns such as loss of cultural identity due to field abandonment were raised, especially in Manqorholweni, Mawane, and Melani. Homestead gardens were identified as a compensating strategy, with 78% of respondents actively maintaining them, although LULC change results indicated a decline, except in Mawane. Field cultivation revealed gender, age, and educational disparities, with non-cropping households having more female heads, individuals above 55, and higher proportions with only primary education. Households that abandoned field cultivation reported a decline in economic stability, with 97% experiencing less stable household economic circumstances. Unemployment was prevalent, with over 70% relying on social grants, creating financial challenges for 76% lacking sufficient cash. Limited off-farm formal employment (20%) was observed, while remittances played a minor role, this resulted in insufficient monthly income, affecting 94% of respondents. Dietary diversity declined, and reliance on store-bought food led to health concerns. Field abandonment impacted family dynamics, altering power relationships and reducing interaction, also heightened by COVID-19, affecting cultural identity and community cohesion. Youth across villages were not currently engaged in field cultivation or farming but expressed interest in revitalising agriculture. They recognised the socio-economic significance of agriculture and highlighted the importance of capital, resources, and government support. Challenges for youth involvement included lack of information, absence of agricultural advisors, and disparities in access to credit. Lack of support from elders and limited sharing of agricultural knowledge further hindered youth's ability to initiate farming projects. In conclusion, this study highlights the significant issue of arable field abandonment in rural areas of the Eastern Cape and KwaZulu-Natal, reflecting broader socio-economic and environmental transformations. The consistent reduction in arable land across the studied villages, coupled with the rise in grassland cover, underscores a critical shift from traditional agricultural practices to alternative land uses. The abandonment of fields has had profound impacts on community livelihoods, leading to decreased economic stability, reduced dietary diversity, and weakened social and cultural ties. The findings reveal a clear need for targeted interventions to address the specific challenges faced by these communities, particularly regarding food security and economic resilience. To address the challenges of arable field abandonment, it is crucial to implement a multi-faceted support program. This should include providing essential resources such as fencing, irrigation systems, and agricultural tools to facilitate field cultivation. Additionally, engaging and supporting youth in agriculture through training, access to advisors, and financial aid is vital to revitalise interest in farming. Strengthening social safety nets by enhancing financial support systems can mitigate economic challenges faced by affected households. Furthermore, initiating cultural and educational programs to preserve traditional farming knowledge while integrating modern practices will help maintain cultural identity and community cohesion. These measures are key to improving resilience and livelihoods in the context of field abandonment. , Thesis (PhD) -- Faculty of Science, Environmental Science, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Pokwana, Sihle
- Date: 2025-04-03
- Subjects: Arable land , Land abandonment , Livelihood , Rural conditions , Food security South Africa , Land cover , South Africa Economic conditions
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479941 , vital:78382 , DOI 10.21504/10962/479941
- Description: The phenomenon of arable field abandonment, a complex and multifaceted aspect of land use dynamics, has become a subject of increasing significance in rural landscapes. Across diverse geographic contexts, the abandonment of once-cultivated fields is indicative of transformative shifts in agricultural practices, reflecting the interplay of numerous socio-economic, environmental, and cultural factors. The abandonment of arable fields in the former homelands of the Eastern Cape and KwaZulu-Natal poses a multifaceted challenge with significant social implications. Historically, agriculture has been central to these regions, yet recent trends show a shift from traditional field cultivation to alternative practices such as garden cultivation or complete cessation. This shift has heightened vulnerability to food insecurity, hunger, and poverty, particularly as high unemployment levels exacerbate the situation. Moreover, the intricate relationships between humans and their surrounding ecosystems is also affected. Therefore, understanding the drivers of arable field abandonment at the community or household level can reveal localised factors that differ from broader, generalised drivers identified in previous studies. Additionally, examining the diverse impacts on these communities is crucial for developing targeted interventions that address their specific needs and enhance resilience. This study investigates arable field abandonment in specific villages of Gotyibeni and Melani in the Eastern Cape and Manqorholweni and Mawane, in KwaZulu-Natal. Using the Driving forces, Pressures, State, Impacts, and Responses (DPSIR) framework as a conceptual framework uncovers the driving factors, pressures, state, impacts and responses of this phenomenon in these rural villages. The study’s main aim was to assess the social effects of arable field abandonment in the selected study areas. The main objective is to determine how livelihoods, social relationships, and living conditions within and between households have changed following the cessation of field cultivation. The study also employed several specific objectives: first, to identify trends in field cultivation within the study areas; second, to analyse the drivers contributing to arable field abandonment in the villages; third, to examine the impact of field abandonment on the elderly population over the past 50 years; and fourth, to investigate the level of youth engagement in agriculture, including how the abandonment of arable fields affects their participation and perceptions of agriculture as a livelihood. To achieve the objectives the study employed mixed research methods that combine satellite imagery analysis, household surveys, oral history interviews with elders, most of whom had stopped farming, and youth focus group discussions. Landsat 7 Satellite imagery with a 15-meter spatial resolution was sourced from the South African Space Agency (SANSA) for the four study areas. The temporal coverage spanned from 1972 to 2020, with considerations for image usability based on resolution. Unfortunately, many earlier images were deemed unusable due to low resolution, particularly affecting Gotyibeni, where clear images only became available from 2013, therefore for this village the focus was on two decades. Arable fields, residential areas, homestead gardens, grasslands, and shrublands were classified. Change detection utilised a post-classification approach, comparing land cover categories between the early and late 2000s. A change detection matrix and statistics were generated using a ha/year formula, enabling the quantification of land cover changes. Image analysis employed ArcGIS 10.8.1 software for mapping and classification, involving georeferencing, signature file creation, maximum likelihood classification, and conversion from raster to vector formats for area calculations. For the LULC change matrix, an intersection analysis in ArcGIS overlaid data from different time points, and a pivot table in Excel organised resulting data. A focus on arable land changes, particularly conversions to other land uses, was crucial, as the study is investigating the level of arable field abandonment in these areas, understanding how much arable land has been converted or remained unchanged provides a clear indication of the extent of abandonment. To understand driving forces of arable field abandonment, a semi-structured questionnaire was developed based on the Driving forces, Pressures, States, Impacts, and Responses (DPSIR) framework. The questionnaires were administered through household interviews conducted in the four study villages. Sections of the questionnaire covered household involvement in cropping, perceptions of land use change, abandonment of field cultivation, and socio-economic demographics. Sampling involved a random selection of 20% of total households in each village, with data analysis performed using Statistical Package for Social Sciences (SPSS). Chi-square tests and Kruskal-Wallis analysis were employed to assess differences between villages. A second questionnaire specifically targeting households that had abandoned field cultivation was also done. Ninety-seven interviews were conducted to evaluate economic, health, socio-cultural and environmental impacts from respondents that indicated to have ceased field cultivation. Data analysis through SPSS utilised descriptive statistics and chi-square tests to identify statistically significant differences between villages. Oral histories were collected from elderly individuals in each village from a sample size of 15 individuals who were previous cultivators of fields, selected with the assistance of a village leader. The selected elders provided qualitative insights into historical and contemporary experiential aspects of village life. The focus was on changes in village relations, the environment, generational values, and traditional farming practices in each village. Youth-focused group discussions were organised with 30 young people in the village between the ages of 18-35, covering topics such as youth goals, the importance of field cultivation, and perceptions of the future. Both the oral histories and focus group discussions conducted in the local languages, were tape-recorded, transcribed, and subjected to content analysis to identify and interpret themes and patterns. The approach involved coding sentences, grouping codes into sub-themes, and further organising them into categories and subcategories. The LULC change results showed a consistent trend of significant arable land reduction across all villages, accompanied by a notable increase in grassland cover. Manqorholweni experienced the most significant loss of these arable fields. This finding was further supported by the household interviews which revealed 100% abandonment of fields in this village. Grasslands emerged as the dominant land cover in all villages, overtaking arable fields and homestead gardens. Regarding field abandonment, 76% of households across villages left fields unused for over 19 years due to reasons including lack of fencing, animal damage to crops, and financial constraints, despite recognising the socio-economic importance of field cultivation. Respondents expressed interest in returning to cultivation if the state would assist with fencing materials and costs. Concerns such as loss of cultural identity due to field abandonment were raised, especially in Manqorholweni, Mawane, and Melani. Homestead gardens were identified as a compensating strategy, with 78% of respondents actively maintaining them, although LULC change results indicated a decline, except in Mawane. Field cultivation revealed gender, age, and educational disparities, with non-cropping households having more female heads, individuals above 55, and higher proportions with only primary education. Households that abandoned field cultivation reported a decline in economic stability, with 97% experiencing less stable household economic circumstances. Unemployment was prevalent, with over 70% relying on social grants, creating financial challenges for 76% lacking sufficient cash. Limited off-farm formal employment (20%) was observed, while remittances played a minor role, this resulted in insufficient monthly income, affecting 94% of respondents. Dietary diversity declined, and reliance on store-bought food led to health concerns. Field abandonment impacted family dynamics, altering power relationships and reducing interaction, also heightened by COVID-19, affecting cultural identity and community cohesion. Youth across villages were not currently engaged in field cultivation or farming but expressed interest in revitalising agriculture. They recognised the socio-economic significance of agriculture and highlighted the importance of capital, resources, and government support. Challenges for youth involvement included lack of information, absence of agricultural advisors, and disparities in access to credit. Lack of support from elders and limited sharing of agricultural knowledge further hindered youth's ability to initiate farming projects. In conclusion, this study highlights the significant issue of arable field abandonment in rural areas of the Eastern Cape and KwaZulu-Natal, reflecting broader socio-economic and environmental transformations. The consistent reduction in arable land across the studied villages, coupled with the rise in grassland cover, underscores a critical shift from traditional agricultural practices to alternative land uses. The abandonment of fields has had profound impacts on community livelihoods, leading to decreased economic stability, reduced dietary diversity, and weakened social and cultural ties. The findings reveal a clear need for targeted interventions to address the specific challenges faced by these communities, particularly regarding food security and economic resilience. To address the challenges of arable field abandonment, it is crucial to implement a multi-faceted support program. This should include providing essential resources such as fencing, irrigation systems, and agricultural tools to facilitate field cultivation. Additionally, engaging and supporting youth in agriculture through training, access to advisors, and financial aid is vital to revitalise interest in farming. Strengthening social safety nets by enhancing financial support systems can mitigate economic challenges faced by affected households. Furthermore, initiating cultural and educational programs to preserve traditional farming knowledge while integrating modern practices will help maintain cultural identity and community cohesion. These measures are key to improving resilience and livelihoods in the context of field abandonment. , Thesis (PhD) -- Faculty of Science, Environmental Science, 2025
- Full Text:
- Date Issued: 2025-04-03
Exploring academics’ experiences of the research ethical clearance process: a case study of a South African university
- Manqele, Siyanda Mabutho Dominic
- Authors: Manqele, Siyanda Mabutho Dominic
- Date: 2025-04-03
- Subjects: Research Moral and ethical aspects , Graduate students Control South Africa , Compliance , Trust , Community engagement
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/480029 , vital:78390
- Description: This study explores the experiences of academics regarding the research ethical clearance process at a South African university, aiming to identify key challenges and opportunities for improvement. The rationale behind this investigation stems from the increasing emphasis on ethical considerations in research, alongside the potential barriers that may hinder academics from navigating the clearance process effectively. The research questions focus on understanding the perceptions of academic staff concerning compliance, trust in the ethical review process, and the role of education and community engagement in facilitating ethical research practices. Utilising qualitative research methods approach, this study employed thematic analysis alongside qualitative methods, involving semi-structured interviews with 14 academic staff members from six faculties, all possessing a minimum of five years of experience in research and postgraduate supervision. The thematic analysis revealed several critical themes, including compliance challenges, trust and mistrust in the ethical clearance process, the necessity of education and awareness, and the significance of community engagement in fostering a supportive research environment. The findings indicate that compliance with ethical standards is often perceived as burdensome, leading to frustration among academics. Trust issues emerged, highlighting a perceived disconnect between researchers and the ethical review board, which can undermine the integrity of the clearance process. The study underscores the importance of enhancing education and awareness regarding ethical guidelines, suggesting that targeted training programmes could improve understanding and adherence among researchers. Community engagement also emerged as a vital element, with recommendations for fostering collaborative relationships between the university and local communities to enhance ethical research practices. The implications of this study point toward the necessity for universities to reevaluate their ethical clearance processes, promoting transparency, trust, and collaboration. By addressing the identified challenges and fostering a culture of ethical research, universities can support their academics in conducting research that is not only compliant but also socially responsible. Future research could further explore the perspectives of ethical review board members to gain a comprehensive understanding of the ethical clearance landscape. , Thesis (MEd) -- Faculty of Education, Centre for Higher Education Research, Teaching and Learning, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Manqele, Siyanda Mabutho Dominic
- Date: 2025-04-03
- Subjects: Research Moral and ethical aspects , Graduate students Control South Africa , Compliance , Trust , Community engagement
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/480029 , vital:78390
- Description: This study explores the experiences of academics regarding the research ethical clearance process at a South African university, aiming to identify key challenges and opportunities for improvement. The rationale behind this investigation stems from the increasing emphasis on ethical considerations in research, alongside the potential barriers that may hinder academics from navigating the clearance process effectively. The research questions focus on understanding the perceptions of academic staff concerning compliance, trust in the ethical review process, and the role of education and community engagement in facilitating ethical research practices. Utilising qualitative research methods approach, this study employed thematic analysis alongside qualitative methods, involving semi-structured interviews with 14 academic staff members from six faculties, all possessing a minimum of five years of experience in research and postgraduate supervision. The thematic analysis revealed several critical themes, including compliance challenges, trust and mistrust in the ethical clearance process, the necessity of education and awareness, and the significance of community engagement in fostering a supportive research environment. The findings indicate that compliance with ethical standards is often perceived as burdensome, leading to frustration among academics. Trust issues emerged, highlighting a perceived disconnect between researchers and the ethical review board, which can undermine the integrity of the clearance process. The study underscores the importance of enhancing education and awareness regarding ethical guidelines, suggesting that targeted training programmes could improve understanding and adherence among researchers. Community engagement also emerged as a vital element, with recommendations for fostering collaborative relationships between the university and local communities to enhance ethical research practices. The implications of this study point toward the necessity for universities to reevaluate their ethical clearance processes, promoting transparency, trust, and collaboration. By addressing the identified challenges and fostering a culture of ethical research, universities can support their academics in conducting research that is not only compliant but also socially responsible. Future research could further explore the perspectives of ethical review board members to gain a comprehensive understanding of the ethical clearance landscape. , Thesis (MEd) -- Faculty of Education, Centre for Higher Education Research, Teaching and Learning, 2025
- Full Text:
- Date Issued: 2025-04-03
Exploring Foundation Phase pre-service teachers’ mathematical pedagogical content knowledge for teaching additive mental mathematics strategies
- Authors: Kumm, Marisa Luisa
- Date: 2025-04-03
- Subjects: Pedagogical content knowledge , Mathematics Study and teaching (Primary) South Africa , Pre-service teacher education , Student teachers South Africa , Addition Study and teaching (Elementary) , Number concept Study and teaching (Elementary)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/480018 , vital:78389
- Description: Poor learner performance in mathematics remains a concern in South African primary and secondary education according to continuous national and international assessments. Many learners fall behind in the early grades without being provided the opportunity to establish a solid foundational number sense. This is an essential requirement to progress into the higher grades. Addressing the challenges of number sense in the Foundation Phase is mandatory. This study aimed to investigate third-year Foundation Phase pre-service teachers’ content knowledge and pedagogical content knowledge of their additive reasoning strategies (a key aspect of number sense) at a private teacher education institution, guided by the question: What content knowledge and pedagogical content knowledge do third-year Foundation Phase pre-service teachers have of additive reasoning mental mathematics strategies? Several researchers have identified the need to develop pre-service teachers’ mathematical content knowledge and pedagogical content knowledge. This study was guided by Shulman’s (1986) constructs of teacher knowledge. This qualitative, descriptive study, which formed part of the Mental Mathematics – Work Integrated Learning (MM-WIL) programme, collected data from an interpretivist perspective. The information for this study was gathered from participants who agreed to be part of the study. The study collected data through pre- and post-intervention questionnaires that had two parts. The first part assessed pre-service teachers’ methods of solving four basic additive reasoning calculations (that lent themselves to using the strategies of bridging through ten, jump strategy, and rounding and adjusting). The second part of the questionnaire asked pre-service teachers to describe these strategies and how they would teach them, with the aim of understanding their additive reasoning skills and ability to use and teach these mental strategies. Key findings are that many pre-service teachers do not have the content knowledge to solve basic calculations using efficient methods. These are skills that they are required to teach in the Foundation Phase classroom. The findings in this study highlight the urgent need to address the weak content knowledge, pedagogical content knowledge and inefficient unit based counting methods of many pre-service teachers to improve learners’ development and fundamental understanding of numbers that allows for flexible and efficient calculation. , Thesis (MEd) -- Faculty of Education, Primary and Early Childhood Education, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Kumm, Marisa Luisa
- Date: 2025-04-03
- Subjects: Pedagogical content knowledge , Mathematics Study and teaching (Primary) South Africa , Pre-service teacher education , Student teachers South Africa , Addition Study and teaching (Elementary) , Number concept Study and teaching (Elementary)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/480018 , vital:78389
- Description: Poor learner performance in mathematics remains a concern in South African primary and secondary education according to continuous national and international assessments. Many learners fall behind in the early grades without being provided the opportunity to establish a solid foundational number sense. This is an essential requirement to progress into the higher grades. Addressing the challenges of number sense in the Foundation Phase is mandatory. This study aimed to investigate third-year Foundation Phase pre-service teachers’ content knowledge and pedagogical content knowledge of their additive reasoning strategies (a key aspect of number sense) at a private teacher education institution, guided by the question: What content knowledge and pedagogical content knowledge do third-year Foundation Phase pre-service teachers have of additive reasoning mental mathematics strategies? Several researchers have identified the need to develop pre-service teachers’ mathematical content knowledge and pedagogical content knowledge. This study was guided by Shulman’s (1986) constructs of teacher knowledge. This qualitative, descriptive study, which formed part of the Mental Mathematics – Work Integrated Learning (MM-WIL) programme, collected data from an interpretivist perspective. The information for this study was gathered from participants who agreed to be part of the study. The study collected data through pre- and post-intervention questionnaires that had two parts. The first part assessed pre-service teachers’ methods of solving four basic additive reasoning calculations (that lent themselves to using the strategies of bridging through ten, jump strategy, and rounding and adjusting). The second part of the questionnaire asked pre-service teachers to describe these strategies and how they would teach them, with the aim of understanding their additive reasoning skills and ability to use and teach these mental strategies. Key findings are that many pre-service teachers do not have the content knowledge to solve basic calculations using efficient methods. These are skills that they are required to teach in the Foundation Phase classroom. The findings in this study highlight the urgent need to address the weak content knowledge, pedagogical content knowledge and inefficient unit based counting methods of many pre-service teachers to improve learners’ development and fundamental understanding of numbers that allows for flexible and efficient calculation. , Thesis (MEd) -- Faculty of Education, Primary and Early Childhood Education, 2025
- Full Text:
- Date Issued: 2025-04-03
Exploring organisational learning in water resource protection: activity system analysis of social learning among resource directed measures role-players in South Africa
- Authors: Thwala, Mmaphefo Doreen
- Date: 2025-04-03
- Subjects: Social learning , Organizational learning , Soft systems methodology , Water-supply Management , Cultural-historical activity theory
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/480117 , vital:78398 , DOI 10.21504/10962/480117
- Description: Globally, there are policy and practice efforts to mainstream inclusive education in different sub-fields of education. Part of the efforts have focused on investigating and finding practical mechanisms for the mainstreaming process in teacher education. Anecdotally, Education for Sustainable Development (ESD), with its focus on transformative education, collaborative learning, the community of practice and the whole institution approach, has the potential to mainstream inclusive education in teacher education practices if grounded in sociocultural realities rather than mere ideological framing. This situation is related to the need for more transformative capabilities for teacher educators to prepare teachers for inclusive pedagogical proficiency. However, in a southern African context, the outlook of the potential of pedagogical proficiency from the nexus of inclusive education, ESD and teacher education is disjointed. As such, this study focused on investigating the mainstreaming of inclusive education in teacher education practice for pedagogical proficiency through ESD Change Projects in Southern Africa, examining cases from Malawi, Tanzania and Eswatini. The study intended to gain an understanding of inclusive education and ESD in these countries, identify areas for strengthening inclusive education in teacher education practice, collaborate with teacher educators to develop strategies for mainstreaming inclusive education, and generate indicators for monitoring and evaluating inclusive education in teacher education contexts. The study used Urie Bronfenbrenner’s bioecological theory of human development and Lev Vygotsky’s sociocultural theory of learning as theoretical and analytical frameworks. The study also employed Roy Bhaskar’s critical realism philosophy as an underlabourer or a meta-theoretical framework. The study further adapted the Vygotsky-informed Engestrom’s expansive learning as a methodological torch. Together, these frameworks were used to analyse selected Change Projects within Sustainability Starts with Teachers (SST) programme, which focused on ESD capacity building in teacher educators from 11 southern African countries. In this regard, the research utilised a qualitative nested case study design. A formative interventionist research approach supported the cases in Malawi, Tanzania and Eswatini, with the study selecting SST Change Projects dealing with inclusive education through an initial scoping questionnaire. Data were generated through 12 in-depth interviews, nine workshops (involving 24 participants), document reviews, observations and reflective journals. Thematic ii analysis, employing a critical realist approach with abductive and retroductive reasoning, guided the reflexive presentation and discussion of research findings. The research findings revealed a common understanding of inclusive education as providing equal educational opportunities, ESD as supporting sustainable development, and teacher education as skill development. The research findings also highlighted the influence of teacher educators’ biopsychosocial characteristics on their perceptions of inclusive education, ESD and teacher education. Areas requiring improvement in teacher education systems included resources, attitude change, financing, cooperation, collaboration and leadership. Strategies for mainstreaming inclusive education in teacher education practice for pedagogical proficiency included curriculum implementation, policy shift, contextualisation and interactive systems. Monitoring and evaluation in all three cases focused on teaching practice, assessment, feedback tools, forums and curriculum implementation, but the absence of specific indicators for monitoring and evaluation was evident. These findings suggest multi-layered and complex implications for policy formulation, implementation practices and future research. Therefore, this thesis argues that, in a southern Africa context, the potential of pedagogical proficiency from the nexus of inclusive education, ESD and teacher education involves considering biopsychosocial characteristics to facilitate the capabilities of teacher educators. This situation necessitates utilising inclusivity mechanisms in pre-service teacher training grounded in biosocial and psychocultural realities of the region’s educational challenges. Consequently, this thesis proffers the Sustainable Inclusive Pedagogical Proficiency Process (SIP3) model as a framework to actualise the nexus of inclusive education, ESD and teacher education, and bring into focus its pedagogical proficiency potential which eventually helps with the mainstreaming process in teacher education practice via ESD. The study reported in this thesis contributes to international theory and practice development for inclusivity and ESD in teacher education practice, provides indicators for monitoring inclusive quality education in teacher education practice, and introduces a contextual dynamics model for comparative education research. The thesis is structured in eight chapters, introducing the study context, addressing literature gaps, presenting the theoretical framework, detailing research design, exploring specific cases in Malawi, Tanzania and Eswatini, discussing the research findings and its implications, and concluding with recommendations for change and future research. , Thesis (PhD) -- Faculty of Education, Secondary and Post School Education, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Thwala, Mmaphefo Doreen
- Date: 2025-04-03
- Subjects: Social learning , Organizational learning , Soft systems methodology , Water-supply Management , Cultural-historical activity theory
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/480117 , vital:78398 , DOI 10.21504/10962/480117
- Description: Globally, there are policy and practice efforts to mainstream inclusive education in different sub-fields of education. Part of the efforts have focused on investigating and finding practical mechanisms for the mainstreaming process in teacher education. Anecdotally, Education for Sustainable Development (ESD), with its focus on transformative education, collaborative learning, the community of practice and the whole institution approach, has the potential to mainstream inclusive education in teacher education practices if grounded in sociocultural realities rather than mere ideological framing. This situation is related to the need for more transformative capabilities for teacher educators to prepare teachers for inclusive pedagogical proficiency. However, in a southern African context, the outlook of the potential of pedagogical proficiency from the nexus of inclusive education, ESD and teacher education is disjointed. As such, this study focused on investigating the mainstreaming of inclusive education in teacher education practice for pedagogical proficiency through ESD Change Projects in Southern Africa, examining cases from Malawi, Tanzania and Eswatini. The study intended to gain an understanding of inclusive education and ESD in these countries, identify areas for strengthening inclusive education in teacher education practice, collaborate with teacher educators to develop strategies for mainstreaming inclusive education, and generate indicators for monitoring and evaluating inclusive education in teacher education contexts. The study used Urie Bronfenbrenner’s bioecological theory of human development and Lev Vygotsky’s sociocultural theory of learning as theoretical and analytical frameworks. The study also employed Roy Bhaskar’s critical realism philosophy as an underlabourer or a meta-theoretical framework. The study further adapted the Vygotsky-informed Engestrom’s expansive learning as a methodological torch. Together, these frameworks were used to analyse selected Change Projects within Sustainability Starts with Teachers (SST) programme, which focused on ESD capacity building in teacher educators from 11 southern African countries. In this regard, the research utilised a qualitative nested case study design. A formative interventionist research approach supported the cases in Malawi, Tanzania and Eswatini, with the study selecting SST Change Projects dealing with inclusive education through an initial scoping questionnaire. Data were generated through 12 in-depth interviews, nine workshops (involving 24 participants), document reviews, observations and reflective journals. Thematic ii analysis, employing a critical realist approach with abductive and retroductive reasoning, guided the reflexive presentation and discussion of research findings. The research findings revealed a common understanding of inclusive education as providing equal educational opportunities, ESD as supporting sustainable development, and teacher education as skill development. The research findings also highlighted the influence of teacher educators’ biopsychosocial characteristics on their perceptions of inclusive education, ESD and teacher education. Areas requiring improvement in teacher education systems included resources, attitude change, financing, cooperation, collaboration and leadership. Strategies for mainstreaming inclusive education in teacher education practice for pedagogical proficiency included curriculum implementation, policy shift, contextualisation and interactive systems. Monitoring and evaluation in all three cases focused on teaching practice, assessment, feedback tools, forums and curriculum implementation, but the absence of specific indicators for monitoring and evaluation was evident. These findings suggest multi-layered and complex implications for policy formulation, implementation practices and future research. Therefore, this thesis argues that, in a southern Africa context, the potential of pedagogical proficiency from the nexus of inclusive education, ESD and teacher education involves considering biopsychosocial characteristics to facilitate the capabilities of teacher educators. This situation necessitates utilising inclusivity mechanisms in pre-service teacher training grounded in biosocial and psychocultural realities of the region’s educational challenges. Consequently, this thesis proffers the Sustainable Inclusive Pedagogical Proficiency Process (SIP3) model as a framework to actualise the nexus of inclusive education, ESD and teacher education, and bring into focus its pedagogical proficiency potential which eventually helps with the mainstreaming process in teacher education practice via ESD. The study reported in this thesis contributes to international theory and practice development for inclusivity and ESD in teacher education practice, provides indicators for monitoring inclusive quality education in teacher education practice, and introduces a contextual dynamics model for comparative education research. The thesis is structured in eight chapters, introducing the study context, addressing literature gaps, presenting the theoretical framework, detailing research design, exploring specific cases in Malawi, Tanzania and Eswatini, discussing the research findings and its implications, and concluding with recommendations for change and future research. , Thesis (PhD) -- Faculty of Education, Secondary and Post School Education, 2025
- Full Text:
- Date Issued: 2025-04-03
Exploring pedagogical translanguaging practices used by ESL primary school teachers to enhance reading comprehension
- Authors: Mufori, Selma Ndagwedhapo
- Date: 2025-04-03
- Subjects: English as a second or foreign language , Translanguaging (Linguistics) , Reading comprehension , Reading comprehension Study and teaching Namibia , Fourth grade (Education) Namibia
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/479985 , vital:78386 , https://doi.org/10.21504/RUR.28955792.v1
- Description: Namibia has decided to use English as the transitional language and medium of instruction for Grade 4, even though learners have been performing poorly in English Second Language (ESL). Despite various interventions being recommended to address this issue, there has been little success. One teaching approach that experienced ESL teachers may use to enhance reading comprehension is pedagogical translanguaging. However, there is a lack of research exploring the use of this approach in Namibian primary schools, particularly in Grade 4. To address this gap, this study was conducted to investigate the pedagogical translanguaging practices employed by Grade 4 ESL primary school teachers in Namibia to improve reading comprehension. A qualitative exploratory case study oriented to an interpretivist paradigm was conducted. Purposive sampling was used to select five Grade 4 ESL teachers from the Oshikoto region for the study. Data were collected using simulated recall interviews, non-participant observation and document analysis. The data were analysed using an inductive thematic framework. The study showed that using simplified language, gestures, and pictures helped ESL teachers communicate with their learners and improve reading comprehension. The use of visual aids such as picture-rich texts, drawings, sketches and concrete materials (realia) also helped Grade 4 ESL learners in their reading comprehension. This visual support helped in vocabulary acquisition and reinforced the meaning of words through associations with images, leading to a better understanding of the language. The study also found that including drawings and sketches in reading lessons added a layer of pedagogical depth that aligned with learners' cultural backgrounds. This helped teachers to engage with their learners more deeply and promoted a sense of cultural inclusivity in the ESL classroom. The study found that teachers use Oshindonga, the learners’ mother tongue, to communicate with those who struggle with English or find it challenging to understand ESL texts. The study revealed that when teachers encouraged learners to use their mother tongue, they became more active and asked questions for clarity. Moreover, the study revealed that code-switching and language brokering, where Grade 4 emergent ESL learners and the ESL teacher translate or interpret using both the learners' native language and ESL, helped increase learner engagement and comprehension. The findings suggest that using translanguaging practices can enhance comprehension, support learners with special needs and assist in the overall learning and understanding of ESL. Furthermore, the study concluded that Grade 4 ESL teachers employ translanguaging procedures in an unplanned manner, perceiving the use of the mother tongue as a spontaneous action carried out when circumstances require it. This study recommends incorporating translanguaging into the official language policy and the National Curriculum for Basic Education as a means of instruction in ESL classrooms. This official endorsement would guarantee the integration of translanguaging across schools and support consistent language learning. The formalisation and endorsement of translanguaging in the official language policy would prove its success and encourage ESL teachers to utilise it. , Thesis (MEd) -- Faculty of Education, Secondary and Post School Education, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Mufori, Selma Ndagwedhapo
- Date: 2025-04-03
- Subjects: English as a second or foreign language , Translanguaging (Linguistics) , Reading comprehension , Reading comprehension Study and teaching Namibia , Fourth grade (Education) Namibia
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/479985 , vital:78386 , https://doi.org/10.21504/RUR.28955792.v1
- Description: Namibia has decided to use English as the transitional language and medium of instruction for Grade 4, even though learners have been performing poorly in English Second Language (ESL). Despite various interventions being recommended to address this issue, there has been little success. One teaching approach that experienced ESL teachers may use to enhance reading comprehension is pedagogical translanguaging. However, there is a lack of research exploring the use of this approach in Namibian primary schools, particularly in Grade 4. To address this gap, this study was conducted to investigate the pedagogical translanguaging practices employed by Grade 4 ESL primary school teachers in Namibia to improve reading comprehension. A qualitative exploratory case study oriented to an interpretivist paradigm was conducted. Purposive sampling was used to select five Grade 4 ESL teachers from the Oshikoto region for the study. Data were collected using simulated recall interviews, non-participant observation and document analysis. The data were analysed using an inductive thematic framework. The study showed that using simplified language, gestures, and pictures helped ESL teachers communicate with their learners and improve reading comprehension. The use of visual aids such as picture-rich texts, drawings, sketches and concrete materials (realia) also helped Grade 4 ESL learners in their reading comprehension. This visual support helped in vocabulary acquisition and reinforced the meaning of words through associations with images, leading to a better understanding of the language. The study also found that including drawings and sketches in reading lessons added a layer of pedagogical depth that aligned with learners' cultural backgrounds. This helped teachers to engage with their learners more deeply and promoted a sense of cultural inclusivity in the ESL classroom. The study found that teachers use Oshindonga, the learners’ mother tongue, to communicate with those who struggle with English or find it challenging to understand ESL texts. The study revealed that when teachers encouraged learners to use their mother tongue, they became more active and asked questions for clarity. Moreover, the study revealed that code-switching and language brokering, where Grade 4 emergent ESL learners and the ESL teacher translate or interpret using both the learners' native language and ESL, helped increase learner engagement and comprehension. The findings suggest that using translanguaging practices can enhance comprehension, support learners with special needs and assist in the overall learning and understanding of ESL. Furthermore, the study concluded that Grade 4 ESL teachers employ translanguaging procedures in an unplanned manner, perceiving the use of the mother tongue as a spontaneous action carried out when circumstances require it. This study recommends incorporating translanguaging into the official language policy and the National Curriculum for Basic Education as a means of instruction in ESL classrooms. This official endorsement would guarantee the integration of translanguaging across schools and support consistent language learning. The formalisation and endorsement of translanguaging in the official language policy would prove its success and encourage ESL teachers to utilise it. , Thesis (MEd) -- Faculty of Education, Secondary and Post School Education, 2025
- Full Text:
- Date Issued: 2025-04-03
Exploring the development of computational thinking among pre-service teachers using visual programming: an interventionist case study
- Authors: Sepula, Chikondi
- Date: 2025-04-03
- Subjects: Computational thinking , Visual programming (Computer science) , Sociocultural perspective , Problem solving , Scratch (Computer program language) , Student teachers South Africa
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/479996 , vital:78387
- Description: Due to its cognitive effect on learners, computational thinking (CT) has gained significant attention and has been increasingly integrated into primary and secondary education worldwide. The integration of CT into educational curricula offers several benefits, including improved learning outcomes, enhanced problem-solving abilities, and the development of skills necessary for the digital landscape of the 21st century. Recognizing these benefits, South Africa introduced CT in primary schools in 2023 through a dedicated subject, coding and robotics. However, teacher upskilling remains a major challenge, as many teachers lack the necessary skills to teach this subject effectively. This problem is particularly pronounced at the foundational phase, where delivering similar content to young learners presents additional pedagogical complexities. Thus, this study explored the development of CT skills with visual programming among foundational phase pre-service teachers. Situated within the interpretive paradigm, a qualitative case study methodology was employed, owing to its effectiveness in exploring contextual factors and complexities that influence human experiences. The study involved 49 first-year pre-service teachers in an Introduction to Technology module at Rhodes University. Purposive sampling was used to select the foundational phase pre-service teachers at Rhodes University. Data was collected using CT reflective tool, semi-structured interviews, focus-group discussions, and reflective journals. This study was grounded in Vygotsky’s sociocultural theory (SCT) to understand and mediate the development of CT through visual programming. It utilized the “Code, Connect, Create” professional development (PD) model as a structured teacher training approach for CT development through visual programming. Additionally, the “Use, Modify, Create” pedagogical model was implemented as a CT framework specific to visual programming to guide and regulate pedagogical decisions during the intervention. Brennan and Resnick’s 3D CT framework was employed to identify and analyse the CT concepts and skills incorporated in the study. Thematic analysis, which involved coding was used to generate themes from qualitative data to address the research questions. Results indicated that visual programming approach enhances CT by fostering interest, creativity, and collaboration. Key enablers included contextualised project-based learning, a clear rationale for CT, and prior programming exposure. Conversely, lack of a clear CT rationale, prior programming experience, and the multimedia nature of Scratch were identified as hindrances. The study concluded that visual programming effectively enhances CT among foundational phase pre-service teachers and recommended its early integration into their training programs to enhance CT skills. , Thesis (MEd) -- Faculty of Education, Secondary and Post School Education, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Sepula, Chikondi
- Date: 2025-04-03
- Subjects: Computational thinking , Visual programming (Computer science) , Sociocultural perspective , Problem solving , Scratch (Computer program language) , Student teachers South Africa
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/479996 , vital:78387
- Description: Due to its cognitive effect on learners, computational thinking (CT) has gained significant attention and has been increasingly integrated into primary and secondary education worldwide. The integration of CT into educational curricula offers several benefits, including improved learning outcomes, enhanced problem-solving abilities, and the development of skills necessary for the digital landscape of the 21st century. Recognizing these benefits, South Africa introduced CT in primary schools in 2023 through a dedicated subject, coding and robotics. However, teacher upskilling remains a major challenge, as many teachers lack the necessary skills to teach this subject effectively. This problem is particularly pronounced at the foundational phase, where delivering similar content to young learners presents additional pedagogical complexities. Thus, this study explored the development of CT skills with visual programming among foundational phase pre-service teachers. Situated within the interpretive paradigm, a qualitative case study methodology was employed, owing to its effectiveness in exploring contextual factors and complexities that influence human experiences. The study involved 49 first-year pre-service teachers in an Introduction to Technology module at Rhodes University. Purposive sampling was used to select the foundational phase pre-service teachers at Rhodes University. Data was collected using CT reflective tool, semi-structured interviews, focus-group discussions, and reflective journals. This study was grounded in Vygotsky’s sociocultural theory (SCT) to understand and mediate the development of CT through visual programming. It utilized the “Code, Connect, Create” professional development (PD) model as a structured teacher training approach for CT development through visual programming. Additionally, the “Use, Modify, Create” pedagogical model was implemented as a CT framework specific to visual programming to guide and regulate pedagogical decisions during the intervention. Brennan and Resnick’s 3D CT framework was employed to identify and analyse the CT concepts and skills incorporated in the study. Thematic analysis, which involved coding was used to generate themes from qualitative data to address the research questions. Results indicated that visual programming approach enhances CT by fostering interest, creativity, and collaboration. Key enablers included contextualised project-based learning, a clear rationale for CT, and prior programming exposure. Conversely, lack of a clear CT rationale, prior programming experience, and the multimedia nature of Scratch were identified as hindrances. The study concluded that visual programming effectively enhances CT among foundational phase pre-service teachers and recommended its early integration into their training programs to enhance CT skills. , Thesis (MEd) -- Faculty of Education, Secondary and Post School Education, 2025
- Full Text:
- Date Issued: 2025-04-03
Follow the yellow flag road : advancing the biological control of a global wetland invader, and a case study on ecosystem recovery
- Authors: Minuti, Gianmarco
- Date: 2025-04-03
- Subjects: Aquatic plants , Invasive plants Biological control , Iris pseudacorus , Azolla filiculoides , Aphthona nonstriata , Host specificity
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479908 , vital:78378 , DOI 10.21504/10962/479908
- Description: Global change is accelerating habitat degradation and extinction rates, disproportionately affecting freshwater ecosystems. Biological invasions, a core component of global change, have been recognized as a major driver of this phenomenon. Invasive alien aquatic plants (IAAPs) are a prime example, disrupting ecological dynamics and hindering the economic and social activities that depend upon freshwater ecosystems. The best approach to managing IAAPs is preventing their introduction and taking swift action when infestations are detected. Unfortunately, most IAAPs are only recognized after they have spread, making eradication difficult and requiring expensive, ongoing management through manual, mechanical, or chemical means, which become less effective as infestations grow. In this regard, a sustainable alternative against IAAPs is provided by classical biological control. This method lays on the assumption that invasive species have been introduced in an environment which is free from their natural enemies, and that such release allowed them to redirect their resources towards growth and reproduction. Accordingly, weed biocontrol involves introducing one such enemy - a so-called biocontrol agent - to regulate the population of the plant, ideally reducing its density below a critical threshold, minimizing its impacts. Biocontrol programs must follow stringent protocols in order to a) ensure the selection of the most appropriate agents; b) guarantee their establishment and effectiveness in controlling their target; c) minimize the risks involved with their release; and d) monitor their impacts after they have been released. This thesis is composed of two parts, focusing on pre-release studies (Part I, Chapters 1-4) and post-release evaluations (Part II, Chapter 5), respectively. Part I explores the biological control of Iris pseudacorus L. (Iridaceae) – yellow flag – a Eurasian plant that has invaded temperate wetlands worldwide. The first step consisted of conducting field surveys across the native range of the weed, which led to the selection of three candidate biocontrol agents. As a second step, bioclimatic niche modelling was used to forecast the current and future suitability of the weed and its prospective agents. These models helped to identify areas most at risk of invasion and predict how climate change might affect biocontrol efficacy, showing differing impacts in the Northern and Southern Hemispheres. Further, a scoring system was developed to prioritize non-target plants for host-specificity testing, focusing on species within the family Iridaceae. Most taxa prioritized are native to the Americas, South Africa and eastern Asia, highlighting the importance of collaboration across regions when dealing with global invaders with complex phylogeographic distributions. Finally, a germination study confirmed that I. pseudacorus seeds from Argentina germinate more than Belgian seeds, especially in absence of cold stratification, suggesting increased performances of invasive populations and an adaptation to warmer temperatures. Nonetheless, impact assessments conducted on one of the three biocontrol agents demonstrated that it could cause juvenile mortality, suggesting it may effectively reduce seedling recruitment, though further research on agents targeting reproductive structures is recommended. Part II of the thesis addresses an often understudied aspect of post-release evaluations: ecosystem recovery following biological control. To do so, a case study is presented, investigating the biological control of water fern, Azolla filiculoides Lamb. (Salviniaceae), in Belgium. The results of this study are used to discuss the shortcomings of different experimental designs, the lack of baseline data in invasion ecology, and the intricacies of defining and quantifying ecosystem recovery in light of legacy effects and context-dependency. , Wereldwijde verandering versnelt de degradatie van habitats en de uitstervingscijfers, met een onevenredige impact op zoetwaterecosystemen. Biologische invasies, een kernonderdeel van klimaatverandering, worden erkend als een belangrijke drijvende kracht achter dit fenomeen. Invasieve uitheemse waterplanten (IAAPs) zijn een duidelijk voorbeeld, omdat ze ecologische dynamiek verstoren en economische en sociale activiteiten belemmeren die afhankelijk zijn van zoetwaterecosystemen. De beste aanpak voor het beheer van IAAPs is het voorkomen van hun introductie en het nemen van snelle maatregelen bij het detecteren van besmettingen. Helaas worden de meeste IAAPs pas herkend nadat ze zich hebben verspreid, wat uitroeiing moeilijk maakt en dure, voortdurende beheersing vereist door handmatige, mechanische of chemische middelen, die minder effectief worden naarmate besmettingen toenemen. In dit opzicht biedt klassieke biologische bestrijding een duurzaam alternatief tegen IAAPs. Deze methode berust op de veronderstelling dat invasieve soorten zijn geïntroduceerd in een omgeving die vrij is van hun natuurlijke vijanden, waardoor ze hun middelen konden richten op groei en reproductie. Bijgevolg omvat onkruidbestrijding het introduceren van een dergelijke vijand - een zogenaamde biocontrole-agent - om de populatie van de plant te reguleren, idealiter door de dichtheid ervan onder een kritische drempel te verlagen en zo de impact ervan te minimaliseren. Biocontroleprogramma's moeten strikte protocollen volgen om a) de selectie van de meest geschikte agens te waarborgen; b) hun vestiging en effectiviteit bij het beheersen van hun doel te garanderen; c) de risico's van hun vrijlating te minimaliseren; en d) hun impact na vrijlating te monitoren. Dit proefschrift bestaat uit twee delen, gericht op respectievelijk pre-release studies (Deel I, Hoofdstukken 1-4) en post-release evaluaties (Deel II, Hoofdstuk 5). Deel I onderzoekt de biologische bestrijding van Iris pseudacorus L. (Iridaceae) - gele lis - een Euraziatische plant die gematigde moeraslanden wereldwijd heeft gekoloniseerd. De eerste stap bestond uit het uitvoeren van veldonderzoeken in het inheemse verspreidingsgebied van het onkruid, wat leidde tot de selectie van drie kandidaat-biocontroleagenten. Als tweede stap werd bioklimatisch nis-modelleren gebruikt om de huidige en toekomstige geschiktheid van het onkruid en zijn potentiële agens te voorspellen. Deze modellen hielpen gebieden met het grootste risico op invasie te identificeren en voorspelden hoe klimaatverandering de effectiviteit van biocontrole zou kunnen beïnvloeden, met verschillende effecten op het noordelijk en zuidelijk halfrond. Daarnaast werd een scoresysteem ontwikkeld om niet-doelplanten te prioriteren voor host-specifieke tests, met de nadruk op soorten binnen de familie Iridaceae. De prioritaire taxa zijn inheems in Amerika, Zuid-Afrika en Oost-Azië, wat het belang van samenwerking tussen regio’s onderstreept bij het omgaan met mondiale invasieve soorten met complexe fylogeografische verspreiding. Ten slotte bevestigde een kiemingsstudie dat I. pseudacorus zaden uit Argentinië meer kiemen dan Belgische zaden, vooral bij afwezigheid van koude stratificatie, wat wijst op verhoogde prestaties van invasieve populaties en aanpassing aan warmere temperaturen. Nochtans toonden effectbeoordelingen van een van de drie biocontroleagens aan dat deze de mortaliteit van juvenielen kon veroorzaken, wat suggereert dat het effectief kan zijn bij het verminderen van de zaailingrekrutering. Toch wordt verder onderzoek naar agenten die zich richten op reproductieve structuren aanbevolen. Deel II van het proefschrift behandelt een vaak onderbelicht aspect van post-release evaluaties: ecosysteemherstel na biologische bestrijding. Om dit te realiseren wordt een casestudy aangeboden over de biologische bestrijding van watervaren, Azolla filiculoides Lamb. (Salviniaceae), in België. De resultaten van deze studie worden gebruikt voor de bespreking van de tekortkomingen van verschillende experimentele ontwerpen, het gebrek aan basisgegevens in invasie-ecologie en de complexiteit van het definiëren en kwantificeren van ecosysteemherstel in het licht van nalatenschapseffecten en contextafhankelijkheid. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Minuti, Gianmarco
- Date: 2025-04-03
- Subjects: Aquatic plants , Invasive plants Biological control , Iris pseudacorus , Azolla filiculoides , Aphthona nonstriata , Host specificity
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479908 , vital:78378 , DOI 10.21504/10962/479908
- Description: Global change is accelerating habitat degradation and extinction rates, disproportionately affecting freshwater ecosystems. Biological invasions, a core component of global change, have been recognized as a major driver of this phenomenon. Invasive alien aquatic plants (IAAPs) are a prime example, disrupting ecological dynamics and hindering the economic and social activities that depend upon freshwater ecosystems. The best approach to managing IAAPs is preventing their introduction and taking swift action when infestations are detected. Unfortunately, most IAAPs are only recognized after they have spread, making eradication difficult and requiring expensive, ongoing management through manual, mechanical, or chemical means, which become less effective as infestations grow. In this regard, a sustainable alternative against IAAPs is provided by classical biological control. This method lays on the assumption that invasive species have been introduced in an environment which is free from their natural enemies, and that such release allowed them to redirect their resources towards growth and reproduction. Accordingly, weed biocontrol involves introducing one such enemy - a so-called biocontrol agent - to regulate the population of the plant, ideally reducing its density below a critical threshold, minimizing its impacts. Biocontrol programs must follow stringent protocols in order to a) ensure the selection of the most appropriate agents; b) guarantee their establishment and effectiveness in controlling their target; c) minimize the risks involved with their release; and d) monitor their impacts after they have been released. This thesis is composed of two parts, focusing on pre-release studies (Part I, Chapters 1-4) and post-release evaluations (Part II, Chapter 5), respectively. Part I explores the biological control of Iris pseudacorus L. (Iridaceae) – yellow flag – a Eurasian plant that has invaded temperate wetlands worldwide. The first step consisted of conducting field surveys across the native range of the weed, which led to the selection of three candidate biocontrol agents. As a second step, bioclimatic niche modelling was used to forecast the current and future suitability of the weed and its prospective agents. These models helped to identify areas most at risk of invasion and predict how climate change might affect biocontrol efficacy, showing differing impacts in the Northern and Southern Hemispheres. Further, a scoring system was developed to prioritize non-target plants for host-specificity testing, focusing on species within the family Iridaceae. Most taxa prioritized are native to the Americas, South Africa and eastern Asia, highlighting the importance of collaboration across regions when dealing with global invaders with complex phylogeographic distributions. Finally, a germination study confirmed that I. pseudacorus seeds from Argentina germinate more than Belgian seeds, especially in absence of cold stratification, suggesting increased performances of invasive populations and an adaptation to warmer temperatures. Nonetheless, impact assessments conducted on one of the three biocontrol agents demonstrated that it could cause juvenile mortality, suggesting it may effectively reduce seedling recruitment, though further research on agents targeting reproductive structures is recommended. Part II of the thesis addresses an often understudied aspect of post-release evaluations: ecosystem recovery following biological control. To do so, a case study is presented, investigating the biological control of water fern, Azolla filiculoides Lamb. (Salviniaceae), in Belgium. The results of this study are used to discuss the shortcomings of different experimental designs, the lack of baseline data in invasion ecology, and the intricacies of defining and quantifying ecosystem recovery in light of legacy effects and context-dependency. , Wereldwijde verandering versnelt de degradatie van habitats en de uitstervingscijfers, met een onevenredige impact op zoetwaterecosystemen. Biologische invasies, een kernonderdeel van klimaatverandering, worden erkend als een belangrijke drijvende kracht achter dit fenomeen. Invasieve uitheemse waterplanten (IAAPs) zijn een duidelijk voorbeeld, omdat ze ecologische dynamiek verstoren en economische en sociale activiteiten belemmeren die afhankelijk zijn van zoetwaterecosystemen. De beste aanpak voor het beheer van IAAPs is het voorkomen van hun introductie en het nemen van snelle maatregelen bij het detecteren van besmettingen. Helaas worden de meeste IAAPs pas herkend nadat ze zich hebben verspreid, wat uitroeiing moeilijk maakt en dure, voortdurende beheersing vereist door handmatige, mechanische of chemische middelen, die minder effectief worden naarmate besmettingen toenemen. In dit opzicht biedt klassieke biologische bestrijding een duurzaam alternatief tegen IAAPs. Deze methode berust op de veronderstelling dat invasieve soorten zijn geïntroduceerd in een omgeving die vrij is van hun natuurlijke vijanden, waardoor ze hun middelen konden richten op groei en reproductie. Bijgevolg omvat onkruidbestrijding het introduceren van een dergelijke vijand - een zogenaamde biocontrole-agent - om de populatie van de plant te reguleren, idealiter door de dichtheid ervan onder een kritische drempel te verlagen en zo de impact ervan te minimaliseren. Biocontroleprogramma's moeten strikte protocollen volgen om a) de selectie van de meest geschikte agens te waarborgen; b) hun vestiging en effectiviteit bij het beheersen van hun doel te garanderen; c) de risico's van hun vrijlating te minimaliseren; en d) hun impact na vrijlating te monitoren. Dit proefschrift bestaat uit twee delen, gericht op respectievelijk pre-release studies (Deel I, Hoofdstukken 1-4) en post-release evaluaties (Deel II, Hoofdstuk 5). Deel I onderzoekt de biologische bestrijding van Iris pseudacorus L. (Iridaceae) - gele lis - een Euraziatische plant die gematigde moeraslanden wereldwijd heeft gekoloniseerd. De eerste stap bestond uit het uitvoeren van veldonderzoeken in het inheemse verspreidingsgebied van het onkruid, wat leidde tot de selectie van drie kandidaat-biocontroleagenten. Als tweede stap werd bioklimatisch nis-modelleren gebruikt om de huidige en toekomstige geschiktheid van het onkruid en zijn potentiële agens te voorspellen. Deze modellen hielpen gebieden met het grootste risico op invasie te identificeren en voorspelden hoe klimaatverandering de effectiviteit van biocontrole zou kunnen beïnvloeden, met verschillende effecten op het noordelijk en zuidelijk halfrond. Daarnaast werd een scoresysteem ontwikkeld om niet-doelplanten te prioriteren voor host-specifieke tests, met de nadruk op soorten binnen de familie Iridaceae. De prioritaire taxa zijn inheems in Amerika, Zuid-Afrika en Oost-Azië, wat het belang van samenwerking tussen regio’s onderstreept bij het omgaan met mondiale invasieve soorten met complexe fylogeografische verspreiding. Ten slotte bevestigde een kiemingsstudie dat I. pseudacorus zaden uit Argentinië meer kiemen dan Belgische zaden, vooral bij afwezigheid van koude stratificatie, wat wijst op verhoogde prestaties van invasieve populaties en aanpassing aan warmere temperaturen. Nochtans toonden effectbeoordelingen van een van de drie biocontroleagens aan dat deze de mortaliteit van juvenielen kon veroorzaken, wat suggereert dat het effectief kan zijn bij het verminderen van de zaailingrekrutering. Toch wordt verder onderzoek naar agenten die zich richten op reproductieve structuren aanbevolen. Deel II van het proefschrift behandelt een vaak onderbelicht aspect van post-release evaluaties: ecosysteemherstel na biologische bestrijding. Om dit te realiseren wordt een casestudy aangeboden over de biologische bestrijding van watervaren, Azolla filiculoides Lamb. (Salviniaceae), in België. De resultaten van deze studie worden gebruikt voor de bespreking van de tekortkomingen van verschillende experimentele ontwerpen, het gebrek aan basisgegevens in invasie-ecologie en de complexiteit van het definiëren en kwantificeren van ecosysteemherstel in het licht van nalatenschapseffecten en contextafhankelijkheid. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2025
- Full Text:
- Date Issued: 2025-04-03
Investigating the basis of legitimation of English literary studies: a case study of a curriculum at a South African University
- Authors: Knoetze, Retha
- Date: 2025-04-03
- Subjects: Knowledge, Theory of , Critical thinking , English literature Study and teaching (Higher) South Africa , Neoliberalism South Africa , Critical literacy , Transformative learning
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/480050 , vital:78392 , DOI 10.21504/10962/480050
- Description: This study explored the kinds of knowledge, ways of knowing and ways of being that are valued in English literary studies. It did so by providing an analysis of what was needed to succeed in a specific English literary studies curriculum. The study used the Specialisation dimension of Legitimation Code Theory (LCT) to investigate what is legitimated in an English literature curriculum at the University of South Africa (UNISA) across three years of undergraduate study. The purpose of this analysis was twofold. Firstly, it aimed to make the academic literacy practices of English literary studies more explicit in order to inform pedagogy intended to enable epistemological and ontological access to the discipline. Secondly, the study aimed to facilitate critiques of the curriculum from a social justice perspective by finding ways to make the basis for legitimacy (the ways of being and knowing that are valued) in the curriculum more explicit to both the academics and the students. The study found that English literary studies, as practised at UNISA, was underpinned by what LCT refers to as a ‘cultivated gaze’. This aligns with the findings of previous LCT studies that looked at English literary studies using the dimension of Specialisation. A discipline that is underpinned by a cultivated gaze requires students to exhibit a specific disposition that develops through immersion in the field over an extended period in order to be considered a legitimate knower. The study also found that two orientations within the cultivated gaze were legitimated in the curriculum: an aesthetic orientation and a socio-critical orientation. This finding adds to the previous research because it helps us to better understand the kinds of dispositions that are valued in English literary studies and how these dispositions are cultivated over time. In addition, the study found that neoliberal factors such as massification, managerialism and academic casualisation caused misalignments between the intended curriculum and the practices employed to teach and assess the curriculum. This placed particular limitations on one of the aims of the curriculum which was to cultivate a socially oriented criticality. This finding has implications for how we teach Humanities curricula that aim to develop critical citizens. , Thesis (PhD) -- Faculty of Education, Centre for Higher Education Research, Teaching and Learning, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Knoetze, Retha
- Date: 2025-04-03
- Subjects: Knowledge, Theory of , Critical thinking , English literature Study and teaching (Higher) South Africa , Neoliberalism South Africa , Critical literacy , Transformative learning
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/480050 , vital:78392 , DOI 10.21504/10962/480050
- Description: This study explored the kinds of knowledge, ways of knowing and ways of being that are valued in English literary studies. It did so by providing an analysis of what was needed to succeed in a specific English literary studies curriculum. The study used the Specialisation dimension of Legitimation Code Theory (LCT) to investigate what is legitimated in an English literature curriculum at the University of South Africa (UNISA) across three years of undergraduate study. The purpose of this analysis was twofold. Firstly, it aimed to make the academic literacy practices of English literary studies more explicit in order to inform pedagogy intended to enable epistemological and ontological access to the discipline. Secondly, the study aimed to facilitate critiques of the curriculum from a social justice perspective by finding ways to make the basis for legitimacy (the ways of being and knowing that are valued) in the curriculum more explicit to both the academics and the students. The study found that English literary studies, as practised at UNISA, was underpinned by what LCT refers to as a ‘cultivated gaze’. This aligns with the findings of previous LCT studies that looked at English literary studies using the dimension of Specialisation. A discipline that is underpinned by a cultivated gaze requires students to exhibit a specific disposition that develops through immersion in the field over an extended period in order to be considered a legitimate knower. The study also found that two orientations within the cultivated gaze were legitimated in the curriculum: an aesthetic orientation and a socio-critical orientation. This finding adds to the previous research because it helps us to better understand the kinds of dispositions that are valued in English literary studies and how these dispositions are cultivated over time. In addition, the study found that neoliberal factors such as massification, managerialism and academic casualisation caused misalignments between the intended curriculum and the practices employed to teach and assess the curriculum. This placed particular limitations on one of the aims of the curriculum which was to cultivate a socially oriented criticality. This finding has implications for how we teach Humanities curricula that aim to develop critical citizens. , Thesis (PhD) -- Faculty of Education, Centre for Higher Education Research, Teaching and Learning, 2025
- Full Text:
- Date Issued: 2025-04-03
Investigating the mainstreaming of inclusive education in Teacher Education Practice for Pedagogical Proficiency through Education for Sustainable Development Change Projects in Southern Africa
- Authors: Souza, Ben
- Date: 2025-04-03
- Subjects: Inclusive education South Africa , Education for sustainable development , Teachers Training of South Africa , Teacher effectiveness , Critical realism , Social learning , Bioecological model
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/480106 , vital:78397 , DOI 10.21504/10962/480106
- Description: Globally, there are policy and practice efforts to mainstream inclusive education in different sub-fields of education. Part of the efforts have focused on investigating and finding practical mechanisms for the mainstreaming process in teacher education. Anecdotally, Education for Sustainable Development (ESD), with its focus on transformative education, collaborative learning, the community of practice and the whole institution approach, has the potential to mainstream inclusive education in teacher education practices if grounded in sociocultural realities rather than mere ideological framing. This situation is related to the need for more transformative capabilities for teacher educators to prepare teachers for inclusive pedagogical proficiency. However, in a southern African context, the outlook of the potential of pedagogical proficiency from the nexus of inclusive education, ESD and teacher education is disjointed. As such, this study focused on investigating the mainstreaming of inclusive education in teacher education practice for pedagogical proficiency through ESD Change Projects in Southern Africa, examining cases from Malawi, Tanzania and Eswatini. The study intended to gain an understanding of inclusive education and ESD in these countries, identify areas for strengthening inclusive education in teacher education practice, collaborate with teacher educators to develop strategies for mainstreaming inclusive education, and generate indicators for monitoring and evaluating inclusive education in teacher education contexts. The study used Urie Bronfenbrenner’s bioecological theory of human development and Lev Vygotsky’s sociocultural theory of learning as theoretical and analytical frameworks. The study also employed Roy Bhaskar’s critical realism philosophy as an underlabourer or a meta-theoretical framework. The study further adapted the Vygotsky-informed Engestrom’s expansive learning as a methodological torch. Together, these frameworks were used to analyse selected Change Projects within Sustainability Starts with Teachers (SST) programme, which focused on ESD capacity building in teacher educators from 11 southern African countries. In this regard, the research utilised a qualitative nested case study design. A formative interventionist research approach supported the cases in Malawi, Tanzania and Eswatini, with the study selecting SST Change Projects dealing with inclusive education through an initial scoping questionnaire. Data were generated through 12 in-depth interviews, nine workshops (involving 24 participants), document reviews, observations and reflective journals. Thematic ii analysis, employing a critical realist approach with abductive and retroductive reasoning, guided the reflexive presentation and discussion of research findings. The research findings revealed a common understanding of inclusive education as providing equal educational opportunities, ESD as supporting sustainable development, and teacher education as skill development. The research findings also highlighted the influence of teacher educators’ biopsychosocial characteristics on their perceptions of inclusive education, ESD and teacher education. Areas requiring improvement in teacher education systems included resources, attitude change, financing, cooperation, collaboration and leadership. Strategies for mainstreaming inclusive education in teacher education practice for pedagogical proficiency included curriculum implementation, policy shift, contextualisation and interactive systems. Monitoring and evaluation in all three cases focused on teaching practice, assessment, feedback tools, forums and curriculum implementation, but the absence of specific indicators for monitoring and evaluation was evident. These findings suggest multi-layered and complex implications for policy formulation, implementation practices and future research. Therefore, this thesis argues that, in a southern Africa context, the potential of pedagogical proficiency from the nexus of inclusive education, ESD and teacher education involves considering biopsychosocial characteristics to facilitate the capabilities of teacher educators. This situation necessitates utilising inclusivity mechanisms in pre-service teacher training grounded in biosocial and psychocultural realities of the region’s educational challenges. Consequently, this thesis proffers the Sustainable Inclusive Pedagogical Proficiency Process (SIP3) model as a framework to actualise the nexus of inclusive education, ESD and teacher education, and bring into focus its pedagogical proficiency potential which eventually helps with the mainstreaming process in teacher education practice via ESD. The study reported in this thesis contributes to international theory and practice development for inclusivity and ESD in teacher education practice, provides indicators for monitoring inclusive quality education in teacher education practice, and introduces a contextual dynamics model for comparative education research. The thesis is structured in eight chapters, introducing the study context, addressing literature gaps, presenting the theoretical framework, detailing research design, exploring specific cases in Malawi, Tanzania and Eswatini, discussing the research findings and its implications, and concluding with recommendations for change and future research. , Thesis (PhD) -- Faculty of Education, Secondary and Post School Education, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Souza, Ben
- Date: 2025-04-03
- Subjects: Inclusive education South Africa , Education for sustainable development , Teachers Training of South Africa , Teacher effectiveness , Critical realism , Social learning , Bioecological model
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/480106 , vital:78397 , DOI 10.21504/10962/480106
- Description: Globally, there are policy and practice efforts to mainstream inclusive education in different sub-fields of education. Part of the efforts have focused on investigating and finding practical mechanisms for the mainstreaming process in teacher education. Anecdotally, Education for Sustainable Development (ESD), with its focus on transformative education, collaborative learning, the community of practice and the whole institution approach, has the potential to mainstream inclusive education in teacher education practices if grounded in sociocultural realities rather than mere ideological framing. This situation is related to the need for more transformative capabilities for teacher educators to prepare teachers for inclusive pedagogical proficiency. However, in a southern African context, the outlook of the potential of pedagogical proficiency from the nexus of inclusive education, ESD and teacher education is disjointed. As such, this study focused on investigating the mainstreaming of inclusive education in teacher education practice for pedagogical proficiency through ESD Change Projects in Southern Africa, examining cases from Malawi, Tanzania and Eswatini. The study intended to gain an understanding of inclusive education and ESD in these countries, identify areas for strengthening inclusive education in teacher education practice, collaborate with teacher educators to develop strategies for mainstreaming inclusive education, and generate indicators for monitoring and evaluating inclusive education in teacher education contexts. The study used Urie Bronfenbrenner’s bioecological theory of human development and Lev Vygotsky’s sociocultural theory of learning as theoretical and analytical frameworks. The study also employed Roy Bhaskar’s critical realism philosophy as an underlabourer or a meta-theoretical framework. The study further adapted the Vygotsky-informed Engestrom’s expansive learning as a methodological torch. Together, these frameworks were used to analyse selected Change Projects within Sustainability Starts with Teachers (SST) programme, which focused on ESD capacity building in teacher educators from 11 southern African countries. In this regard, the research utilised a qualitative nested case study design. A formative interventionist research approach supported the cases in Malawi, Tanzania and Eswatini, with the study selecting SST Change Projects dealing with inclusive education through an initial scoping questionnaire. Data were generated through 12 in-depth interviews, nine workshops (involving 24 participants), document reviews, observations and reflective journals. Thematic ii analysis, employing a critical realist approach with abductive and retroductive reasoning, guided the reflexive presentation and discussion of research findings. The research findings revealed a common understanding of inclusive education as providing equal educational opportunities, ESD as supporting sustainable development, and teacher education as skill development. The research findings also highlighted the influence of teacher educators’ biopsychosocial characteristics on their perceptions of inclusive education, ESD and teacher education. Areas requiring improvement in teacher education systems included resources, attitude change, financing, cooperation, collaboration and leadership. Strategies for mainstreaming inclusive education in teacher education practice for pedagogical proficiency included curriculum implementation, policy shift, contextualisation and interactive systems. Monitoring and evaluation in all three cases focused on teaching practice, assessment, feedback tools, forums and curriculum implementation, but the absence of specific indicators for monitoring and evaluation was evident. These findings suggest multi-layered and complex implications for policy formulation, implementation practices and future research. Therefore, this thesis argues that, in a southern Africa context, the potential of pedagogical proficiency from the nexus of inclusive education, ESD and teacher education involves considering biopsychosocial characteristics to facilitate the capabilities of teacher educators. This situation necessitates utilising inclusivity mechanisms in pre-service teacher training grounded in biosocial and psychocultural realities of the region’s educational challenges. Consequently, this thesis proffers the Sustainable Inclusive Pedagogical Proficiency Process (SIP3) model as a framework to actualise the nexus of inclusive education, ESD and teacher education, and bring into focus its pedagogical proficiency potential which eventually helps with the mainstreaming process in teacher education practice via ESD. The study reported in this thesis contributes to international theory and practice development for inclusivity and ESD in teacher education practice, provides indicators for monitoring inclusive quality education in teacher education practice, and introduces a contextual dynamics model for comparative education research. The thesis is structured in eight chapters, introducing the study context, addressing literature gaps, presenting the theoretical framework, detailing research design, exploring specific cases in Malawi, Tanzania and Eswatini, discussing the research findings and its implications, and concluding with recommendations for change and future research. , Thesis (PhD) -- Faculty of Education, Secondary and Post School Education, 2025
- Full Text:
- Date Issued: 2025-04-03
Mainstreaming equitable decision-making under uncertainty at the Water User Association level using a reallocation model in the Western Cape, South Africa
- Authors: Xoxo, Beauten Sinetemba
- Date: 2025-04-03
- Subjects: Water-supply Management , Water-supply South Africa Western Cape , Right to water South Africa Western Cape , Water Distribution , Water resources development South Africa Western Cape , Uncertainty (Information theory) , Hydrology Statistical methods
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479963 , vital:78384 , DOI 10.21504/10962/479963
- Description: This thesis demonstrates how uncertainty can be explicitly incorporated into equitable decision-making in ungauged basins to support fair water reallocation strategies among conflicting uses and reduce vulnerability to water shortages. First, a methodological framework is developed to collect the required socio-economic data. Second, a role-playing game (RPG) is developed in collaboration with stakeholders to increase awareness and assess the implications of different allocation strategies and stakeholder actions. Thirdly, a reallocation decision-support system (Water-Sharing Tool) with dam storage and uncertainty is tested to inform strategic water planning under dry conditions. The study was carried out in collaboration with stakeholders in the upper reaches of the Koue Bokkeveld, Western Cape, South Africa. The study area is a winter rainfall area with commercial farming activities targeting the domestic and cross-border markets. Irrigation from numerous farm dams and run-of-river extraction compete with in-stream environmental protection targets for streamflow. The study area can be described as resource-poor in terms of institutional capacity, with water management decisions taken individually at a farm level. The key water users are the environment, farmers, lifestyle farmers (residents), and weekenders. The farmer group has three sub-groups: corporate-owned farms, family-owned commercial farms, and downstream less well-resourced farmers. Results from the user risk profiles show that the least influential actors reside downstream and are more vulnerable to water shortages, which could be attributed to upstream developments and their productivity-driven nature. The thesis pulls together the socio-economic data, the information contributed by the stakeholders during the RPG, uncertain natural runoff estimates, and water demands. It evaluates these using the Water Sharing Model to map water users’ vulnerability under four different management strategies and assesses equitable reallocation outcomes of the proposed strategies to different users. The magnitude and frequency of decision risks and the underlying uncertainty in the water supply are quantified. As expected, results suggest peak risks during months with the lowest streamflow, with negative implications for fruit production in the catchment. Results also showed the negative supply effects of upstream infrastructure development on downstream users and ecosystems. Game results with the farmers suggested different crop choices in dry periods between upstream and downstream farmers. Downstream farmers were surprisingly more willing to forego their dry season entitlements under water-sharing strategies that resulted in serious production losses upstream, prioritising social stability over their own profits. Farmers reflected on the game as an educational tool to enhance system understanding. The study confirms that decision-makers' understanding of the implications of water allocation decisions and the surrounding uncertainty is critical to meeting justice/fairness objectives. , Thesis (PhD) -- Faculty of Science, Institute for Water Research, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Xoxo, Beauten Sinetemba
- Date: 2025-04-03
- Subjects: Water-supply Management , Water-supply South Africa Western Cape , Right to water South Africa Western Cape , Water Distribution , Water resources development South Africa Western Cape , Uncertainty (Information theory) , Hydrology Statistical methods
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479963 , vital:78384 , DOI 10.21504/10962/479963
- Description: This thesis demonstrates how uncertainty can be explicitly incorporated into equitable decision-making in ungauged basins to support fair water reallocation strategies among conflicting uses and reduce vulnerability to water shortages. First, a methodological framework is developed to collect the required socio-economic data. Second, a role-playing game (RPG) is developed in collaboration with stakeholders to increase awareness and assess the implications of different allocation strategies and stakeholder actions. Thirdly, a reallocation decision-support system (Water-Sharing Tool) with dam storage and uncertainty is tested to inform strategic water planning under dry conditions. The study was carried out in collaboration with stakeholders in the upper reaches of the Koue Bokkeveld, Western Cape, South Africa. The study area is a winter rainfall area with commercial farming activities targeting the domestic and cross-border markets. Irrigation from numerous farm dams and run-of-river extraction compete with in-stream environmental protection targets for streamflow. The study area can be described as resource-poor in terms of institutional capacity, with water management decisions taken individually at a farm level. The key water users are the environment, farmers, lifestyle farmers (residents), and weekenders. The farmer group has three sub-groups: corporate-owned farms, family-owned commercial farms, and downstream less well-resourced farmers. Results from the user risk profiles show that the least influential actors reside downstream and are more vulnerable to water shortages, which could be attributed to upstream developments and their productivity-driven nature. The thesis pulls together the socio-economic data, the information contributed by the stakeholders during the RPG, uncertain natural runoff estimates, and water demands. It evaluates these using the Water Sharing Model to map water users’ vulnerability under four different management strategies and assesses equitable reallocation outcomes of the proposed strategies to different users. The magnitude and frequency of decision risks and the underlying uncertainty in the water supply are quantified. As expected, results suggest peak risks during months with the lowest streamflow, with negative implications for fruit production in the catchment. Results also showed the negative supply effects of upstream infrastructure development on downstream users and ecosystems. Game results with the farmers suggested different crop choices in dry periods between upstream and downstream farmers. Downstream farmers were surprisingly more willing to forego their dry season entitlements under water-sharing strategies that resulted in serious production losses upstream, prioritising social stability over their own profits. Farmers reflected on the game as an educational tool to enhance system understanding. The study confirms that decision-makers' understanding of the implications of water allocation decisions and the surrounding uncertainty is critical to meeting justice/fairness objectives. , Thesis (PhD) -- Faculty of Science, Institute for Water Research, 2025
- Full Text:
- Date Issued: 2025-04-03
New insights on classical radio galaxies from MeerKAT and uGMRT
- Authors: Legodi, Portia Potasi
- Date: 2025-04-03
- Subjects: Radio galaxies , MeerKAT , Giant Metrewave Radio Telescope , Radio sources (Astronomy) , Radio interferometers
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479886 , vital:78376 , DOI 10.21504/10962/479886
- Description: The morphology of low and high-power sources has been the subject of a classification method for over four decades, leading to a comprehensive understanding of extragalactic radio sources. The morphological classification of radio galaxies is again a hot topic. This project aims to revisit the current classification scheme for classical radio galaxies and investigate the properties and origin of the new filamentary features which are becoming standard within and outside the radio lobes and which are suggestive of so far unexplored interactions between the radio plasma and the external medium. This thesis presents high-resolution and sensitivity studies of the nature of ten known radio galaxies: three FRIs (4C -03.43, 3C 403.1, 3C 198), three FRIIs (3C 105, 3C 227, 3C 445), tailed radio sources (CGCG046-067, NGC7503) and the FR0s (SDSS J 09157+1331, SDSS J 1120+0407). The total sample was selected from the 4C catalogue with well-defined criteria. To this aim, we are using the μJy sensitivity offered by the combination of uGMRT and MeerKAT in a frequency range from 500−1712 MHz. The radio images present several new morphological features in the radio emission at a ∼4′′ −10′′ angular resolution. Our radio maps validate the morphological classification. The sources exhibit remarkable features that raise new questions regarding the complex interaction between radio plasma emitted by host galaxies and the surrounding medium through which the jet propagates. The wealth of morphological details includes, for example, the detection of filaments in the emission from the lobes, the existence of diffuse emission beyond the hotspots, and multiple knot-like structures along the jet spine in some sources in the sample. Detailed descriptions of the overall radio structures are presented. We derived the equipartition parameters for each source in the sample, and after fitting their integrated spectrum, we estimated their global radiative ages. We find a broad range of radiative ages, i.e. 40.19−242.85 Myr. We derived in-band spectral index imaging for a few radio galaxies in the sample. This allowed us to make some preliminary considerations on the origin of the various radio features. , Thesis (PhD) -- Faculty of Science, Physics and Electronics, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Legodi, Portia Potasi
- Date: 2025-04-03
- Subjects: Radio galaxies , MeerKAT , Giant Metrewave Radio Telescope , Radio sources (Astronomy) , Radio interferometers
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479886 , vital:78376 , DOI 10.21504/10962/479886
- Description: The morphology of low and high-power sources has been the subject of a classification method for over four decades, leading to a comprehensive understanding of extragalactic radio sources. The morphological classification of radio galaxies is again a hot topic. This project aims to revisit the current classification scheme for classical radio galaxies and investigate the properties and origin of the new filamentary features which are becoming standard within and outside the radio lobes and which are suggestive of so far unexplored interactions between the radio plasma and the external medium. This thesis presents high-resolution and sensitivity studies of the nature of ten known radio galaxies: three FRIs (4C -03.43, 3C 403.1, 3C 198), three FRIIs (3C 105, 3C 227, 3C 445), tailed radio sources (CGCG046-067, NGC7503) and the FR0s (SDSS J 09157+1331, SDSS J 1120+0407). The total sample was selected from the 4C catalogue with well-defined criteria. To this aim, we are using the μJy sensitivity offered by the combination of uGMRT and MeerKAT in a frequency range from 500−1712 MHz. The radio images present several new morphological features in the radio emission at a ∼4′′ −10′′ angular resolution. Our radio maps validate the morphological classification. The sources exhibit remarkable features that raise new questions regarding the complex interaction between radio plasma emitted by host galaxies and the surrounding medium through which the jet propagates. The wealth of morphological details includes, for example, the detection of filaments in the emission from the lobes, the existence of diffuse emission beyond the hotspots, and multiple knot-like structures along the jet spine in some sources in the sample. Detailed descriptions of the overall radio structures are presented. We derived the equipartition parameters for each source in the sample, and after fitting their integrated spectrum, we estimated their global radiative ages. We find a broad range of radiative ages, i.e. 40.19−242.85 Myr. We derived in-band spectral index imaging for a few radio galaxies in the sample. This allowed us to make some preliminary considerations on the origin of the various radio features. , Thesis (PhD) -- Faculty of Science, Physics and Electronics, 2025
- Full Text:
- Date Issued: 2025-04-03
Physiological responses of savanna grasses, encroaching, and non-encroaching tree saplings to changing water availability and elevated atmospheric CO2
- Authors: Singili, Edith Joana
- Date: 2025-04-03
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479952 , vital:78383
- Description: Access restricted. Expected release in 2027. , Thesis (PhD) -- Faculty of Science, Botany, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Singili, Edith Joana
- Date: 2025-04-03
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479952 , vital:78383
- Description: Access restricted. Expected release in 2027. , Thesis (PhD) -- Faculty of Science, Botany, 2025
- Full Text:
- Date Issued: 2025-04-03
Scholar-activist transdisciplinary research praxis for blue justice in South Africa: perspectives from the South African Coastal Justice Network scholar-activist archive
- Authors: Pereira-Kaplan, Taryn Leigh
- Date: 2025-04-03
- Subjects: Student movements South Africa , Environmental justice , Ocean governance , Blue justice , Small-scale fisheries South Africa , Interdisciplinary research
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/480095 , vital:78396 , DOI 10.21504/10962/480095
- Description: This PhD thesis is an applied study on the research praxis of transdisciplinary (TD) scholar-activists contributing to social movements for environmental justice on the coasts and in the oceans (‘blue justice’). I wrote this thesis from my position as the coordinator of the Coastal Justice Network (CJN), which is a component of the Global Challenge Research Fund One Ocean Hub’s TD ocean governance programme. The CJN is a grouping of South African TD scholar-activists working collaboratively in processes of knowledge co-production with small-scale fishers (SSFs) and other coastal communities. These SSFs are leading resistances to blue injustice from the margins of ocean governance. Within a solidarity and environmental justice orientation to TD ocean research, CJN researchers and SSFs have responded together to a wide range of blue justice issues between 2020 and 2024 and, in doing so, have co-generated an activist archive. This thesis draws on the activist archive to surface core practices and priorities for research that contribute to movements for blue justice. The study was developed as a PhD portfolio through five papers, with an introduction and conclusion. The main aim of this study was to explore dimensions of scholar-activist TD research praxis and associated contributions to advancing blue justice in transformative ocean governance. In doing this, it sought to address some of the gaps in blue justice TD research, most notably the need for a deeper understanding of how to centre the voices and contributions of those most affected by environmental justice concerns. It also addresses the role of scholar-activist researchers practised as a form of political solidarity and reflexive co-engagement. The main research question is: How can scholar-activist transdisciplinary research praxis contribute to advancing blue justice in transformative ocean governance in South Africa? Methodologically, the study uses a form of activist ethnography, which is a scholar-activist methodology that includes an explicit political commitment to engagement and to generating knowledge for activism purposes. Methods within activist ethnographic research include participant and self-observation, critically reflexive “thick” descriptions of context and practice, interviews and conversations, direct political actions with activist partners and facilitation of mutual learning. At the centre of this work is the co-constructed scholar-activist archive, which offers a record of four years of such TD scholar-activist praxis. This thesis and the papers presented as part of the thesis all draw on the co-produced scholar-activist archive constructed out of the social movement work of the SSFs in collaboration with CJN researcher’s TD scholar-activist research praxis over four years, representing the type of activist ethnography referred to above. Compiling and organising the archive was one important level of analytical/synthesis work I undertook. I also drew on the archive to make visible key facets of blue justice work and reflected on this, making up three different levels of analytical work with the archive: 1) Constructing and organising the archive, 2) Selection of key foci in the archive, and 3) Meta-reflections. Through this approach, I address the main research question via four sub-questions, each the focus of a paper in this PhD by publication. Why is there a need to advance scholar-activist TD practice in transformative ocean governance research? This question is addressed in Paper 1 (Chapter 2 of this thesis). How can scholar-activists in blue justice support just and inclusive views of ocean governance? This question is addressed in Paper 2 (Chapter 3 of the thesis). What methods in blue justice research enable plural knowledges and perspectives for co-engagement? This question is addressed in Paper 3 (Chapter 4 of the thesis). How is blue justice resistance expressed and acknowledged as a key feature of inclusive ocean governance? This question is addressed in Paper 4 (Chapter 5 of the thesis). What emerges as key lessons for scholar-activist TD researchers in blue justice? This question is addressed in Paper 5 (Chapter 6 of the thesis) and in the meta-reflection in Chapter 7. The thesis as a whole offers: 1. Identification of a core practice, centred on “transformative space making” for care- ful, responsive and reflexive solidarity networks – ‘net-work’ – that allows community-based activists and social movements to leverage the kinds of research support they need when they need it. 2. Insight into participatory ocean governance and socially just ocean protection, practised through an ‘agonistic’ and counter-hegemonic knowledge co-production emergent from this form of TD scholar-activism. 3. Methods and guidance for these practices, with specific emphasis on agonistically plural and inclusive methods of blue resistance. 4. Insights into the positionality and ethical tensions of TD scholar-activist researchers. The study offers an empirical case of how a CJN, through TD scholar-activist praxis, can contribute to blue resistance and blue justice. The reflective chapter (Chapter 7) shows that this type of TD scholar-activist praxis is not without challenges and limitations; through a reflexive review of these, the study offers direction for further research. It also points to the roles of scholar-activists working in solidarity with SSFs in pursuit of blue justice. Overall, the thesis offers an orientation for TD researchers interested in aligning their research praxis with social movements working in counter-hegemonic ways for environmental justice. , Thesis (PhD) -- Faculty of Education, Secondary and Post School Education, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Pereira-Kaplan, Taryn Leigh
- Date: 2025-04-03
- Subjects: Student movements South Africa , Environmental justice , Ocean governance , Blue justice , Small-scale fisheries South Africa , Interdisciplinary research
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/480095 , vital:78396 , DOI 10.21504/10962/480095
- Description: This PhD thesis is an applied study on the research praxis of transdisciplinary (TD) scholar-activists contributing to social movements for environmental justice on the coasts and in the oceans (‘blue justice’). I wrote this thesis from my position as the coordinator of the Coastal Justice Network (CJN), which is a component of the Global Challenge Research Fund One Ocean Hub’s TD ocean governance programme. The CJN is a grouping of South African TD scholar-activists working collaboratively in processes of knowledge co-production with small-scale fishers (SSFs) and other coastal communities. These SSFs are leading resistances to blue injustice from the margins of ocean governance. Within a solidarity and environmental justice orientation to TD ocean research, CJN researchers and SSFs have responded together to a wide range of blue justice issues between 2020 and 2024 and, in doing so, have co-generated an activist archive. This thesis draws on the activist archive to surface core practices and priorities for research that contribute to movements for blue justice. The study was developed as a PhD portfolio through five papers, with an introduction and conclusion. The main aim of this study was to explore dimensions of scholar-activist TD research praxis and associated contributions to advancing blue justice in transformative ocean governance. In doing this, it sought to address some of the gaps in blue justice TD research, most notably the need for a deeper understanding of how to centre the voices and contributions of those most affected by environmental justice concerns. It also addresses the role of scholar-activist researchers practised as a form of political solidarity and reflexive co-engagement. The main research question is: How can scholar-activist transdisciplinary research praxis contribute to advancing blue justice in transformative ocean governance in South Africa? Methodologically, the study uses a form of activist ethnography, which is a scholar-activist methodology that includes an explicit political commitment to engagement and to generating knowledge for activism purposes. Methods within activist ethnographic research include participant and self-observation, critically reflexive “thick” descriptions of context and practice, interviews and conversations, direct political actions with activist partners and facilitation of mutual learning. At the centre of this work is the co-constructed scholar-activist archive, which offers a record of four years of such TD scholar-activist praxis. This thesis and the papers presented as part of the thesis all draw on the co-produced scholar-activist archive constructed out of the social movement work of the SSFs in collaboration with CJN researcher’s TD scholar-activist research praxis over four years, representing the type of activist ethnography referred to above. Compiling and organising the archive was one important level of analytical/synthesis work I undertook. I also drew on the archive to make visible key facets of blue justice work and reflected on this, making up three different levels of analytical work with the archive: 1) Constructing and organising the archive, 2) Selection of key foci in the archive, and 3) Meta-reflections. Through this approach, I address the main research question via four sub-questions, each the focus of a paper in this PhD by publication. Why is there a need to advance scholar-activist TD practice in transformative ocean governance research? This question is addressed in Paper 1 (Chapter 2 of this thesis). How can scholar-activists in blue justice support just and inclusive views of ocean governance? This question is addressed in Paper 2 (Chapter 3 of the thesis). What methods in blue justice research enable plural knowledges and perspectives for co-engagement? This question is addressed in Paper 3 (Chapter 4 of the thesis). How is blue justice resistance expressed and acknowledged as a key feature of inclusive ocean governance? This question is addressed in Paper 4 (Chapter 5 of the thesis). What emerges as key lessons for scholar-activist TD researchers in blue justice? This question is addressed in Paper 5 (Chapter 6 of the thesis) and in the meta-reflection in Chapter 7. The thesis as a whole offers: 1. Identification of a core practice, centred on “transformative space making” for care- ful, responsive and reflexive solidarity networks – ‘net-work’ – that allows community-based activists and social movements to leverage the kinds of research support they need when they need it. 2. Insight into participatory ocean governance and socially just ocean protection, practised through an ‘agonistic’ and counter-hegemonic knowledge co-production emergent from this form of TD scholar-activism. 3. Methods and guidance for these practices, with specific emphasis on agonistically plural and inclusive methods of blue resistance. 4. Insights into the positionality and ethical tensions of TD scholar-activist researchers. The study offers an empirical case of how a CJN, through TD scholar-activist praxis, can contribute to blue resistance and blue justice. The reflective chapter (Chapter 7) shows that this type of TD scholar-activist praxis is not without challenges and limitations; through a reflexive review of these, the study offers direction for further research. It also points to the roles of scholar-activists working in solidarity with SSFs in pursuit of blue justice. Overall, the thesis offers an orientation for TD researchers interested in aligning their research praxis with social movements working in counter-hegemonic ways for environmental justice. , Thesis (PhD) -- Faculty of Education, Secondary and Post School Education, 2025
- Full Text:
- Date Issued: 2025-04-03
Stimulating Namibian learners’ democratic participation and leadership in a senior primary rural school: a participatory action research study
- Authors: Kaongo, Trofimus Shipena
- Date: 2025-04-03
- Subjects: Student participation in administration Namibia , Democratic participation , Participatory action research , Education, Elementary Namibia , Agent (Philosophy)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/479974 , vital:78385
- Description: This study investigates the democratic participation and leadership opportunities of primary school learners within the Namibian education system, as outlined in the Basic Education Act. 3 of 2020. While the Act emphasises the involvement of secondary school learners in decision making, primary school learners are largely overlooked. Despite policies recognising children as democratic participants in education, implementation remains inconsistent, with secondary school learners often restricted to tokenistic leadership roles. To address this imbalance, the study explores leadership opportunities for primary school learners, seeking to understand the underlying factors contributing to the gap in democratic leadership at the primary school level. Grounded in a critical paradigm, this qualitative research draws upon Kurt Lewin's field theory and theory of change, using participatory action research (PAR) to promote transformation. The research followed a two-phase design. In phase 1, data was collected through individual interviews with teachers and focus group discussions with learners. Phase 2 adopted PAR to assess how engaging learners in the research process stimulated democratic participation and leadership development. Data were gathered through reflective journals maintained by both the learners and the researcher. Abductive analysis of the data, informed by Lewin’s theoretical framework, the Lundy model of participation, Hart’s ladder of participation and leadership theory, revealed varying understandings of democratic participation among participants. For many, it was equated with learners' freedom of expression, collective decision making and leadership autonomy. However, while learners were afforded opportunities to lead school-initiated activities such as cleaning campaigns, sports events and class monitoring, their involvement in the initiation of these activities remained limited. The study found that learner leadership could be significantly enhanced through greater involvement in collective decision making, motivation and recognition by teachers, and increased leadership autonomy. Nonetheless, barriers such as age differences, peer pressure, limited time and teachers’ negative attitudes impeded the realisation of these objectives. The study contributes significant insights into learner leadership in Africa, particularly in the under-researched context of senior primary school leadership. It highlights the need for greater equity in leadership opportunities between primary and secondary learners and advocates a transformative school environment that fosters multiple leadership pathways based on individual cognitive development. , Thesis (MEd) -- Faculty of Education, Secondary and Post School Education, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Kaongo, Trofimus Shipena
- Date: 2025-04-03
- Subjects: Student participation in administration Namibia , Democratic participation , Participatory action research , Education, Elementary Namibia , Agent (Philosophy)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/479974 , vital:78385
- Description: This study investigates the democratic participation and leadership opportunities of primary school learners within the Namibian education system, as outlined in the Basic Education Act. 3 of 2020. While the Act emphasises the involvement of secondary school learners in decision making, primary school learners are largely overlooked. Despite policies recognising children as democratic participants in education, implementation remains inconsistent, with secondary school learners often restricted to tokenistic leadership roles. To address this imbalance, the study explores leadership opportunities for primary school learners, seeking to understand the underlying factors contributing to the gap in democratic leadership at the primary school level. Grounded in a critical paradigm, this qualitative research draws upon Kurt Lewin's field theory and theory of change, using participatory action research (PAR) to promote transformation. The research followed a two-phase design. In phase 1, data was collected through individual interviews with teachers and focus group discussions with learners. Phase 2 adopted PAR to assess how engaging learners in the research process stimulated democratic participation and leadership development. Data were gathered through reflective journals maintained by both the learners and the researcher. Abductive analysis of the data, informed by Lewin’s theoretical framework, the Lundy model of participation, Hart’s ladder of participation and leadership theory, revealed varying understandings of democratic participation among participants. For many, it was equated with learners' freedom of expression, collective decision making and leadership autonomy. However, while learners were afforded opportunities to lead school-initiated activities such as cleaning campaigns, sports events and class monitoring, their involvement in the initiation of these activities remained limited. The study found that learner leadership could be significantly enhanced through greater involvement in collective decision making, motivation and recognition by teachers, and increased leadership autonomy. Nonetheless, barriers such as age differences, peer pressure, limited time and teachers’ negative attitudes impeded the realisation of these objectives. The study contributes significant insights into learner leadership in Africa, particularly in the under-researched context of senior primary school leadership. It highlights the need for greater equity in leadership opportunities between primary and secondary learners and advocates a transformative school environment that fosters multiple leadership pathways based on individual cognitive development. , Thesis (MEd) -- Faculty of Education, Secondary and Post School Education, 2025
- Full Text:
- Date Issued: 2025-04-03
Three decades of change: exploring colonial legacies and shifts within processes of admission at South African Universities of Technology
- Kemm-Stols, Lyndall Elizabeth
- Authors: Kemm-Stols, Lyndall Elizabeth
- Date: 2025-04-03
- Subjects: Education, Higher South Africa , Technical education South Africa , Universities and colleges Admission , Decolonization , Social realism , Educational equalization South Africa
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/480040 , vital:78391 , DOI 10.21504/10962/480040
- Description: Given South Africa’s complex legacy of inequality, access to higher education remains a priority for the government, higher education institutions and the public. And while significant progress has been made in terms of increased participation and expanded opportunities, particularly at Universities of Technology (UoTs), disparities remain. This particular type of institution has played a key role in contributing to the redress agenda through their less stringent admission requirements, but they remain underexamined in broader higher education literature. Despite these contributions, marginalised or working-class communities in rural and impoverished areas continue to face significant challenges in accessing higher education. Set against a global backdrop where similar patterns of admission have emerged, it becomes important to understand how and why the processes of admission in UoTs have emerged through the last three decades. In so doing, this study seeks to contribute to the conversation around social justice in higher education, offering insights that could reshape the understanding of why things are the way they are. This study draws on Critical Realism more broadly and the tools of Social Realism specifically to explore how South Africa’s particular colonial legacy remains one of many hidden mechanisms on which we build our reality. Using various documentation and qualitative data generated through interviews, which was analysed using Social Realism’s morphogenetic framework, I have endeavoured to tell the story of how, Universities of Technologies and their processes of admission have changed over the last three decades. In so doing I have shown, through using a metaphor of ‘The House Modernity Built’ how although much change has happened, hidden structures and cultures have worked to maintain a certain status quo, thereby constraining redress. This study has outlined the emergence of UoTs and their processes of admission as we understand them today. Through this I have presented an argument which suggests that the higher education sector continues to be structured in a hierarchical manner with UoTs located at the bottom. Mechanisms manifesting from a broader neoliberal environment, such as the higher education funding formula and global university ranking systems have placed UoTs in a position where they need to ‘catch up’, having consequences on the way in which processes of admission have been conceptualised. This has seen English becoming a marker of quality, and diploma offerings being replaced with degree equivalents carrying higher admission criteria, all of which work to compromise the redress needed in South African higher education. , Thesis (PhD) -- Faculty of Education, Centre for Higher Education Research, Teaching and Learning, 2025
- Full Text:
- Date Issued: 2025-04-03
- Authors: Kemm-Stols, Lyndall Elizabeth
- Date: 2025-04-03
- Subjects: Education, Higher South Africa , Technical education South Africa , Universities and colleges Admission , Decolonization , Social realism , Educational equalization South Africa
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/480040 , vital:78391 , DOI 10.21504/10962/480040
- Description: Given South Africa’s complex legacy of inequality, access to higher education remains a priority for the government, higher education institutions and the public. And while significant progress has been made in terms of increased participation and expanded opportunities, particularly at Universities of Technology (UoTs), disparities remain. This particular type of institution has played a key role in contributing to the redress agenda through their less stringent admission requirements, but they remain underexamined in broader higher education literature. Despite these contributions, marginalised or working-class communities in rural and impoverished areas continue to face significant challenges in accessing higher education. Set against a global backdrop where similar patterns of admission have emerged, it becomes important to understand how and why the processes of admission in UoTs have emerged through the last three decades. In so doing, this study seeks to contribute to the conversation around social justice in higher education, offering insights that could reshape the understanding of why things are the way they are. This study draws on Critical Realism more broadly and the tools of Social Realism specifically to explore how South Africa’s particular colonial legacy remains one of many hidden mechanisms on which we build our reality. Using various documentation and qualitative data generated through interviews, which was analysed using Social Realism’s morphogenetic framework, I have endeavoured to tell the story of how, Universities of Technologies and their processes of admission have changed over the last three decades. In so doing I have shown, through using a metaphor of ‘The House Modernity Built’ how although much change has happened, hidden structures and cultures have worked to maintain a certain status quo, thereby constraining redress. This study has outlined the emergence of UoTs and their processes of admission as we understand them today. Through this I have presented an argument which suggests that the higher education sector continues to be structured in a hierarchical manner with UoTs located at the bottom. Mechanisms manifesting from a broader neoliberal environment, such as the higher education funding formula and global university ranking systems have placed UoTs in a position where they need to ‘catch up’, having consequences on the way in which processes of admission have been conceptualised. This has seen English becoming a marker of quality, and diploma offerings being replaced with degree equivalents carrying higher admission criteria, all of which work to compromise the redress needed in South African higher education. , Thesis (PhD) -- Faculty of Education, Centre for Higher Education Research, Teaching and Learning, 2025
- Full Text:
- Date Issued: 2025-04-03
A critical microethnographic investigation of anomalies in a grade 4 reading comprehension activity
- Authors: Kitchen, Tracy Lee
- Date: 2025-04-02
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/478526 , vital:78194
- Description: Access restricted. Expected release date in 2027. , Thesis (PhD) -- Faculty of Humanities, Linguistics and Applied Language Studies, 2025
- Full Text:
- Date Issued: 2025-04-02
- Authors: Kitchen, Tracy Lee
- Date: 2025-04-02
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/478526 , vital:78194
- Description: Access restricted. Expected release date in 2027. , Thesis (PhD) -- Faculty of Humanities, Linguistics and Applied Language Studies, 2025
- Full Text:
- Date Issued: 2025-04-02
A large multiscale detailed modelling of aptamers as anticancer therapeutics
- Authors: Mokgopa, Kabelo Phuti
- Date: 2025-04-02
- Subjects: Aptamer , MicroRNA , Drug discovery , Python (Computer program language) , Molecular dynamics , Cheminformatics , Bioinformatics
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/479174 , vital:78267
- Description: Cancer remains a leading cause of death worldwide, characterized by uncontrolled cell growth and spread. The challenge of effectively treating cancer has spurred interest in novel therapeutic strategies that target specific biological or biochemical mechanisms involved in cancer progression. Although many enzymes have been labelled as inducers of cancer development, microRNAs (miRNAs) are also emerging as significant contributors to cancer progression. This is because miRNAs play a crucial role in regulating gene expression, while cancer develops and grows due to genetic mutations, variations, and alterations. Among these miRNAs, miRNA-10b is notable for its involvement in promoting cancer cell proliferation, migration, and metastasis across various cancers, including breast cancer, glioblastoma, and esophageal squamous cell carcinoma. For this reason, we propose inhibiting miRNA-10b using RNA aptamers as a novel and promising approach for developing new anti-cancer therapeutics. RNA aptamers are short, non-coded, synthetic, and single-stranded nucleic acid molecules capable of binding to a wide range of targets, including metal ions, chemical compounds, proteins, cells, and microorganisms. They are used for a range of applications due to their well-known specificity and selectivity, starting from drug delivery to diagnostics. In this project we aimed to design and discover novel RNA aptamers that can effectively inhibit miRNA-10b using advanced computational methods. However, major challenges were encountered due to the lack of databases or tools available to design and predict secondary and tertiary structures of RNA aptamers at a large scale. Furthermore, no tools were available to perform high throughput virtual screening of these aptamers against macromolecular targets at a large scale. Prompted by that, we developed the T_SELEX program, which encompasses the various algorithms and tools dedicated to designing RNA aptamer sequences, predicting their secondary and tertiary structures, and, lastly, virtually screening aptamers. These algorithms and advanced tools are designed to handle the complexities of aptamer selection and virtual screening. By employing virtual screening methods, the aptamer discovery process was streamlined, offering a cost-effective and efficient alternative to traditional SELEX techniques. Prior to the main purpose application, the T_SELEX program was tested by designing aptamers for targeting HIV-1 protease, and a few applications were also done to assess its aptamer design approach. The study explored RNA aptamer sequences, revealing important insights into nucleotide composition, sequence patterns, and their role in aptamer efficacy and design. Analysis of secondary and tertiary structure predictions showed that Minimum Free Energy (MFE) values do not always correlate with structural compactness or complexity, with aptamers of similar MFE values exhibiting variations in attributes like loop size and guanine content. A novel Sequence Similarity Check (SSC) algorithm is introduced focused on internal sequence comparisons and secondary structures, revealing that aptamers with similar base compositions could have distinct folding states and stability. The Base Randomization Algorithm (BRA) generated RNA aptamer libraries was further benchmarked, highlighting a critical threshold for aptamer length and demonstrating Gaussian distribution in base compositions. Virtual screening of aptamers using the T_SELEX program against pre-miRNA-10b and their mature 5p and 3p arm, identified aptamers557 and 899 as effective binders for the 3p and 5p arms, respectively. Extensive quantum mechanical and molecular dynamics simulations confirmed the stability of the aptamer-RNA complexes. Due to the understanding of the flexibility of these RNA-RNA complexes, we further proposed the stability matrices method as a calculus-based method to evaluate the relative stability of the complexes without being biased during MD analysis. MM-GBSA calculations supported docking results, identifying aptamers like aptamers557, aptamer274 and aptamer734 as strong inhibitors of the 3p arm. Overall, this project has proposed novel approaches for aptamer in silico design and validation, particularly in targeting miRNA-10b for cancer therapy. , Thesis (MSc) -- Faculty of Science, Chemistry, 2025
- Full Text:
- Date Issued: 2025-04-02
- Authors: Mokgopa, Kabelo Phuti
- Date: 2025-04-02
- Subjects: Aptamer , MicroRNA , Drug discovery , Python (Computer program language) , Molecular dynamics , Cheminformatics , Bioinformatics
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/479174 , vital:78267
- Description: Cancer remains a leading cause of death worldwide, characterized by uncontrolled cell growth and spread. The challenge of effectively treating cancer has spurred interest in novel therapeutic strategies that target specific biological or biochemical mechanisms involved in cancer progression. Although many enzymes have been labelled as inducers of cancer development, microRNAs (miRNAs) are also emerging as significant contributors to cancer progression. This is because miRNAs play a crucial role in regulating gene expression, while cancer develops and grows due to genetic mutations, variations, and alterations. Among these miRNAs, miRNA-10b is notable for its involvement in promoting cancer cell proliferation, migration, and metastasis across various cancers, including breast cancer, glioblastoma, and esophageal squamous cell carcinoma. For this reason, we propose inhibiting miRNA-10b using RNA aptamers as a novel and promising approach for developing new anti-cancer therapeutics. RNA aptamers are short, non-coded, synthetic, and single-stranded nucleic acid molecules capable of binding to a wide range of targets, including metal ions, chemical compounds, proteins, cells, and microorganisms. They are used for a range of applications due to their well-known specificity and selectivity, starting from drug delivery to diagnostics. In this project we aimed to design and discover novel RNA aptamers that can effectively inhibit miRNA-10b using advanced computational methods. However, major challenges were encountered due to the lack of databases or tools available to design and predict secondary and tertiary structures of RNA aptamers at a large scale. Furthermore, no tools were available to perform high throughput virtual screening of these aptamers against macromolecular targets at a large scale. Prompted by that, we developed the T_SELEX program, which encompasses the various algorithms and tools dedicated to designing RNA aptamer sequences, predicting their secondary and tertiary structures, and, lastly, virtually screening aptamers. These algorithms and advanced tools are designed to handle the complexities of aptamer selection and virtual screening. By employing virtual screening methods, the aptamer discovery process was streamlined, offering a cost-effective and efficient alternative to traditional SELEX techniques. Prior to the main purpose application, the T_SELEX program was tested by designing aptamers for targeting HIV-1 protease, and a few applications were also done to assess its aptamer design approach. The study explored RNA aptamer sequences, revealing important insights into nucleotide composition, sequence patterns, and their role in aptamer efficacy and design. Analysis of secondary and tertiary structure predictions showed that Minimum Free Energy (MFE) values do not always correlate with structural compactness or complexity, with aptamers of similar MFE values exhibiting variations in attributes like loop size and guanine content. A novel Sequence Similarity Check (SSC) algorithm is introduced focused on internal sequence comparisons and secondary structures, revealing that aptamers with similar base compositions could have distinct folding states and stability. The Base Randomization Algorithm (BRA) generated RNA aptamer libraries was further benchmarked, highlighting a critical threshold for aptamer length and demonstrating Gaussian distribution in base compositions. Virtual screening of aptamers using the T_SELEX program against pre-miRNA-10b and their mature 5p and 3p arm, identified aptamers557 and 899 as effective binders for the 3p and 5p arms, respectively. Extensive quantum mechanical and molecular dynamics simulations confirmed the stability of the aptamer-RNA complexes. Due to the understanding of the flexibility of these RNA-RNA complexes, we further proposed the stability matrices method as a calculus-based method to evaluate the relative stability of the complexes without being biased during MD analysis. MM-GBSA calculations supported docking results, identifying aptamers like aptamers557, aptamer274 and aptamer734 as strong inhibitors of the 3p arm. Overall, this project has proposed novel approaches for aptamer in silico design and validation, particularly in targeting miRNA-10b for cancer therapy. , Thesis (MSc) -- Faculty of Science, Chemistry, 2025
- Full Text:
- Date Issued: 2025-04-02
An evaluation of commercially available and extemporaneously prepared topical cannabidiol products within the context of the South African regulatory framework
- Authors: Bennett, Alyson Sebastiane
- Date: 2025-04-02
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479208 , vital:78270
- Description: Access restricted. Expected release date in 2026. , Thesis (PhD) -- Faculty of Pharmcy, Pharmacy, 2025
- Full Text:
- Date Issued: 2025-04-02
- Authors: Bennett, Alyson Sebastiane
- Date: 2025-04-02
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/479208 , vital:78270
- Description: Access restricted. Expected release date in 2026. , Thesis (PhD) -- Faculty of Pharmcy, Pharmacy, 2025
- Full Text:
- Date Issued: 2025-04-02
An investigation on the effects of Afrocentric missense variations on the structure and function of CYP2A6 protein
- Authors: Makombe, Chipo Perpetual
- Date: 2025-04-02
- Subjects: Missense mutation , Structural dynamics , Enzyme activity , Drugs Metabolism , CYP2A6
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/479119 , vital:78262
- Description: Pharmacogenomics, the foundation of personalized medicine distinguishes patients into different categories based on their response to the risk of a disease. Cytochrome P450 (CYPs) proteins are a family of enzymes critical in the metabolism of drugs and other substances. Genetic polymorphisms in CYPs can result in different enzymatic activity in individuals influencing the efficacy and toxicity of drugs. One of the CYPs which primarily metabolizes nicotine and other pharmaceutical drugs such as Artemisinin and Artesunate, Pilocarpine, Valproic Acid and Letrozole is CYP2A6. The gene encoding the protein is highly polymorphic and this can affect the rate of metabolism of drugs in individuals. Previously most studies unveiled connections between CYP2A6 variants and nicotine. Implications concerning the effects of specific missense variations in CYP2A6 drug metabolism have deficiencies. This study aimed to critically examine the structural and functional implications of 13 CYP2A6 allele variations on CYP2A6 protein using Bioinformatics techniques. Methods used were template selection, mutagenesis, parameter assignment and protonation. Molecular Dynamics to get insights regarding protein behavior at an atomic level, clustering to identify conformations during a simulation and DSSP for secondary structure analysis to monitor how secondary structures evolve. Berendsen and Parinello-Rahman barostats at production run were used for comparison. A global analysis was conducted to identify structural transitions (RMSD, RMSF, and Rg), clustering, and secondary structure prediction. Results from Berendsen barostat were inconsistent compared to Parrinello-Rahman barostat implying that CYP2A6 is sensitive to the pressure coupling parameter for precise and accurate results. Our clustering results showed each system in one conformation, fluctuations and shifts on the C-D, H-I loops and F, G, and L helices on variants I149M, F118l, K476R, and E390K_N418D_E419D. This indicated a potential loss of function limiting the protein’s ability to conformational flexibility for catalysis and substrate recognition. Certain regions of CYP2A6 became more rigid due to variations, which could have a negative impact on the catalytic activity, regulatory interactions, and general function of the enzyme in metabolism. Globally the variations did not cause large changes to the protein, there is need for a local analysis using Dynamic Residue Networks to study how residue interactions affect the function of CYP2A6. , Thesis (MSc) -- Faculty of Science, Biochemistry, Microbiology and Bioinformatics, 2025
- Full Text:
- Date Issued: 2025-04-02
- Authors: Makombe, Chipo Perpetual
- Date: 2025-04-02
- Subjects: Missense mutation , Structural dynamics , Enzyme activity , Drugs Metabolism , CYP2A6
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/479119 , vital:78262
- Description: Pharmacogenomics, the foundation of personalized medicine distinguishes patients into different categories based on their response to the risk of a disease. Cytochrome P450 (CYPs) proteins are a family of enzymes critical in the metabolism of drugs and other substances. Genetic polymorphisms in CYPs can result in different enzymatic activity in individuals influencing the efficacy and toxicity of drugs. One of the CYPs which primarily metabolizes nicotine and other pharmaceutical drugs such as Artemisinin and Artesunate, Pilocarpine, Valproic Acid and Letrozole is CYP2A6. The gene encoding the protein is highly polymorphic and this can affect the rate of metabolism of drugs in individuals. Previously most studies unveiled connections between CYP2A6 variants and nicotine. Implications concerning the effects of specific missense variations in CYP2A6 drug metabolism have deficiencies. This study aimed to critically examine the structural and functional implications of 13 CYP2A6 allele variations on CYP2A6 protein using Bioinformatics techniques. Methods used were template selection, mutagenesis, parameter assignment and protonation. Molecular Dynamics to get insights regarding protein behavior at an atomic level, clustering to identify conformations during a simulation and DSSP for secondary structure analysis to monitor how secondary structures evolve. Berendsen and Parinello-Rahman barostats at production run were used for comparison. A global analysis was conducted to identify structural transitions (RMSD, RMSF, and Rg), clustering, and secondary structure prediction. Results from Berendsen barostat were inconsistent compared to Parrinello-Rahman barostat implying that CYP2A6 is sensitive to the pressure coupling parameter for precise and accurate results. Our clustering results showed each system in one conformation, fluctuations and shifts on the C-D, H-I loops and F, G, and L helices on variants I149M, F118l, K476R, and E390K_N418D_E419D. This indicated a potential loss of function limiting the protein’s ability to conformational flexibility for catalysis and substrate recognition. Certain regions of CYP2A6 became more rigid due to variations, which could have a negative impact on the catalytic activity, regulatory interactions, and general function of the enzyme in metabolism. Globally the variations did not cause large changes to the protein, there is need for a local analysis using Dynamic Residue Networks to study how residue interactions affect the function of CYP2A6. , Thesis (MSc) -- Faculty of Science, Biochemistry, Microbiology and Bioinformatics, 2025
- Full Text:
- Date Issued: 2025-04-02