Visual based finger interactions for mobile phones
- Authors: Kerr, Simon
- Date: 2010 , 2010-03-15
- Subjects: User interfaces (Computer systems) , Mobile communication systems -- Design and construction , Cell phones -- Software , Mobile communication systems -- Technological innovations , Information display systems , Cell phones -- Technological innovations
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4652 , http://hdl.handle.net/10962/d1006621 , User interfaces (Computer systems) , Mobile communication systems -- Design and construction , Cell phones -- Software , Mobile communication systems -- Technological innovations , Information display systems , Cell phones -- Technological innovations
- Description: Vision based technology such as motion detection has long been limited to the domain of powerful processor intensive systems such as desktop PCs and specialist hardware solutions. With the advent of much faster mobile phone processors and memory, a plethora of feature rich software and hardware is being deployed onto the mobile platform, most notably onto high powered devices called smart phones. Interaction interfaces such as touchscreens allow for improved usability but obscure the phone’s screen. Since the majority of smart phones are equipped with cameras, it has become feasible to combine their powerful processors, large memory capacity and the camera to support new ways of interacting with the phone which do not obscure the screen. However, it is not clear whether or not these processor intensive visual interactions can in fact be run at an acceptable speed on current mobile handsets or whether they will offer the user a better experience than the current number pad and direction keys present on the majority of mobile phones. A vision based finger interaction technique is proposed which uses the back of device camera to track the user’s finger. This allows the user to interact with the mobile phone with mouse based movements, gestures and steering based interactions. A simple colour thresholding algorithm was implemented in Java, Python and C++. Various benchmarks and tests conducted on a Nokia N95 smart phone revealed that on current hardware and with current programming environments only native C++ yields results plausible for real time interactions (a key requirement for vision based interactions). It is also shown that different lighting levels and background environments affects the accuracy of the system with background and finger contrast playing a large role. Finally a user study was conducted to ascertain the overall user’s satisfaction between keypad interactions and the finger interaction techniques concluding that the new finger interaction technique is well suited to steering based interactions and in time, mouse style movements. Simple navigation is better suited to the directional keypad.
- Full Text:
- Date Issued: 2010
- Authors: Kerr, Simon
- Date: 2010 , 2010-03-15
- Subjects: User interfaces (Computer systems) , Mobile communication systems -- Design and construction , Cell phones -- Software , Mobile communication systems -- Technological innovations , Information display systems , Cell phones -- Technological innovations
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4652 , http://hdl.handle.net/10962/d1006621 , User interfaces (Computer systems) , Mobile communication systems -- Design and construction , Cell phones -- Software , Mobile communication systems -- Technological innovations , Information display systems , Cell phones -- Technological innovations
- Description: Vision based technology such as motion detection has long been limited to the domain of powerful processor intensive systems such as desktop PCs and specialist hardware solutions. With the advent of much faster mobile phone processors and memory, a plethora of feature rich software and hardware is being deployed onto the mobile platform, most notably onto high powered devices called smart phones. Interaction interfaces such as touchscreens allow for improved usability but obscure the phone’s screen. Since the majority of smart phones are equipped with cameras, it has become feasible to combine their powerful processors, large memory capacity and the camera to support new ways of interacting with the phone which do not obscure the screen. However, it is not clear whether or not these processor intensive visual interactions can in fact be run at an acceptable speed on current mobile handsets or whether they will offer the user a better experience than the current number pad and direction keys present on the majority of mobile phones. A vision based finger interaction technique is proposed which uses the back of device camera to track the user’s finger. This allows the user to interact with the mobile phone with mouse based movements, gestures and steering based interactions. A simple colour thresholding algorithm was implemented in Java, Python and C++. Various benchmarks and tests conducted on a Nokia N95 smart phone revealed that on current hardware and with current programming environments only native C++ yields results plausible for real time interactions (a key requirement for vision based interactions). It is also shown that different lighting levels and background environments affects the accuracy of the system with background and finger contrast playing a large role. Finally a user study was conducted to ascertain the overall user’s satisfaction between keypad interactions and the finger interaction techniques concluding that the new finger interaction technique is well suited to steering based interactions and in time, mouse style movements. Simple navigation is better suited to the directional keypad.
- Full Text:
- Date Issued: 2010
A narrative analysis of young black South African women's stories about the recent divorce of their parents
- Authors: Lotter, Jaclyn Oehley
- Date: 2010
- Subjects: Divorce -- South Africa Divorce -- Psychological aspects -- South Africa Children of divorced parents -- South Africa Families -- South Africa Divorce therapy -- South Africa Adolescent psychotherapy -- South Africa Youth, Black -- Counseling of -- South Africa Psychology -- Biographical methods -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3011 , http://hdl.handle.net/10962/d1002520
- Description: The global rise in divorce since the 1960s has brought into question the idealised view of the nuclear family which has for centuries been awarded special status in western societies and has been regarded as the primary social institution. According to contemporary research parental divorce has become a reality for every one in six children in South Africa. Until recently, little consideration had been given to how divorce affects black South African families, as it was considered to be an occurrence which only took place in white, mainly middle-class, families. The proportion of black South African couples divorcing has been increasing over the past decade, and in 2008 was said to contribute to 35% of all divorce in South Africa. Most research to date on the children of divorce has focused on young white children and adolescents and is largely concerned with those who have found parental divorce particularly difficult, and are manifesting adjustment and other behavioural problems. The research which has been done on the effects of parental divorce on young adults focuses mainly on clinical studies with middle-class families based either in the United States or in the United Kingdom. This research, using an experience-centred, life-story narrative approach, explores the stories which young black South African adult women between the ages of 18 and 25 tell, to give account to the ways in which recent parental divorce has affected their lives, views on family life, and what it has meant to them. The use of an experience-centred, life-story narrative approach allows for a process of rupture, acceptance and re-storying to be accounted for, as the participant’s narrative shifts from past, to present and the future. Five young black women from a South African university each participated in two narratively sequenced semi-structured interviews based on McAdams’ personal narratives protocol, which includes six core themes, namely: Key Events, Significant People, Stresses and Problems, Personal Meanings and Life-Lessons, Future Script, and Life Theme. Crossley’s narrative analysis was then used to identify emergent themes and images in each individual narrative, after which they were woven together into a coherent story linked to previous literature. This study found that divorce involves a highly complex transition and reconfiguration process perhaps not fully accounted for in the existing idea and images associated with it. Despite being young adult women who had moved away from home and were engaged in their own lives, it became apparent that parental divorce was still a difficult and complicated experience, but that it is possible to tell both pessimistic and optimistic stories of parental divorce and its consequences.
- Full Text:
- Date Issued: 2010
- Authors: Lotter, Jaclyn Oehley
- Date: 2010
- Subjects: Divorce -- South Africa Divorce -- Psychological aspects -- South Africa Children of divorced parents -- South Africa Families -- South Africa Divorce therapy -- South Africa Adolescent psychotherapy -- South Africa Youth, Black -- Counseling of -- South Africa Psychology -- Biographical methods -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3011 , http://hdl.handle.net/10962/d1002520
- Description: The global rise in divorce since the 1960s has brought into question the idealised view of the nuclear family which has for centuries been awarded special status in western societies and has been regarded as the primary social institution. According to contemporary research parental divorce has become a reality for every one in six children in South Africa. Until recently, little consideration had been given to how divorce affects black South African families, as it was considered to be an occurrence which only took place in white, mainly middle-class, families. The proportion of black South African couples divorcing has been increasing over the past decade, and in 2008 was said to contribute to 35% of all divorce in South Africa. Most research to date on the children of divorce has focused on young white children and adolescents and is largely concerned with those who have found parental divorce particularly difficult, and are manifesting adjustment and other behavioural problems. The research which has been done on the effects of parental divorce on young adults focuses mainly on clinical studies with middle-class families based either in the United States or in the United Kingdom. This research, using an experience-centred, life-story narrative approach, explores the stories which young black South African adult women between the ages of 18 and 25 tell, to give account to the ways in which recent parental divorce has affected their lives, views on family life, and what it has meant to them. The use of an experience-centred, life-story narrative approach allows for a process of rupture, acceptance and re-storying to be accounted for, as the participant’s narrative shifts from past, to present and the future. Five young black women from a South African university each participated in two narratively sequenced semi-structured interviews based on McAdams’ personal narratives protocol, which includes six core themes, namely: Key Events, Significant People, Stresses and Problems, Personal Meanings and Life-Lessons, Future Script, and Life Theme. Crossley’s narrative analysis was then used to identify emergent themes and images in each individual narrative, after which they were woven together into a coherent story linked to previous literature. This study found that divorce involves a highly complex transition and reconfiguration process perhaps not fully accounted for in the existing idea and images associated with it. Despite being young adult women who had moved away from home and were engaged in their own lives, it became apparent that parental divorce was still a difficult and complicated experience, but that it is possible to tell both pessimistic and optimistic stories of parental divorce and its consequences.
- Full Text:
- Date Issued: 2010
Prohibition & resistance: a socio-political exploration of the changing dynamics of the southern African cannabis trade, c. 1850 - the present
- Authors: Paterson, Craig
- Date: 2010
- Subjects: Cannabis -- Government policy -- Africa, Southern -- History -- 19th century Cannabis -- Government policy -- Africa, Southern -- History -- 20th century Cannabis -- Law and legislation -- Africa, Southern -- History -- 20th century Drugs -- Government policy -- Africa, Southern -- History -- 19th century Drugs -- Government policy -- Africa, Southern -- History -- 20th century Drugs -- Law and legislation -- Africa, Southern -- History -- 19th century Drugs -- Law and legislation -- Africa, Southern -- History -- 20th century Drug traffic -- Africa, Southern -- History -- 19th century Drug traffic -- Africa, Southern -- History -- 20th century Substance abuse -- Social aspects -- Africa, Southern
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2551 , http://hdl.handle.net/10962/d1002403
- Description: Looking primarily at the social and political trends in South Africa over the course of the last century and a half, this thesis explores how these trends have contributed to the establishment of the southern Africa cannabis complex. Through an examination of the influence which the colonial paradigm based on Social Darwinian thinking had on the understanding of the cannabis plant in southern Africa, it is argued that cannabis prohibition and apartheid laws rested on the same ideological foundation. This thesis goes on to argue that the dynamics of cannabis production and trade can be understood in terms of the interplay between the two themes of ‘prohibition’ and ‘resistance’. Prohibition is not only understood to refer to cannabis laws, but also to the proscription of inter-racial contact and segregation dictated by the apartheid regime. Resistance, then, refers to both resistance to apartheid and resistance to cannabis laws in this thesis. Including discussions on the hippie movement and development of the world trade, the anti-apartheid movement, the successful implementation of import substitution strategies in Europe and North America from the 1980’s, and South Africa’s incorporation into the global trade, this thesis illustrates how the apartheid system (and its collapse) influenced the region’s cannabis trade.
- Full Text:
- Date Issued: 2010
- Authors: Paterson, Craig
- Date: 2010
- Subjects: Cannabis -- Government policy -- Africa, Southern -- History -- 19th century Cannabis -- Government policy -- Africa, Southern -- History -- 20th century Cannabis -- Law and legislation -- Africa, Southern -- History -- 20th century Drugs -- Government policy -- Africa, Southern -- History -- 19th century Drugs -- Government policy -- Africa, Southern -- History -- 20th century Drugs -- Law and legislation -- Africa, Southern -- History -- 19th century Drugs -- Law and legislation -- Africa, Southern -- History -- 20th century Drug traffic -- Africa, Southern -- History -- 19th century Drug traffic -- Africa, Southern -- History -- 20th century Substance abuse -- Social aspects -- Africa, Southern
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2551 , http://hdl.handle.net/10962/d1002403
- Description: Looking primarily at the social and political trends in South Africa over the course of the last century and a half, this thesis explores how these trends have contributed to the establishment of the southern Africa cannabis complex. Through an examination of the influence which the colonial paradigm based on Social Darwinian thinking had on the understanding of the cannabis plant in southern Africa, it is argued that cannabis prohibition and apartheid laws rested on the same ideological foundation. This thesis goes on to argue that the dynamics of cannabis production and trade can be understood in terms of the interplay between the two themes of ‘prohibition’ and ‘resistance’. Prohibition is not only understood to refer to cannabis laws, but also to the proscription of inter-racial contact and segregation dictated by the apartheid regime. Resistance, then, refers to both resistance to apartheid and resistance to cannabis laws in this thesis. Including discussions on the hippie movement and development of the world trade, the anti-apartheid movement, the successful implementation of import substitution strategies in Europe and North America from the 1980’s, and South Africa’s incorporation into the global trade, this thesis illustrates how the apartheid system (and its collapse) influenced the region’s cannabis trade.
- Full Text:
- Date Issued: 2010
Bioethanol production from waste paper through fungal biotechnology
- Authors: Voigt, Paul George
- Date: 2010
- Subjects: Biomass energy , Cellulose -- Biodegradation , Waste paper -- Recycling , Biomass chemicals -- Economic aspects , Renewable energy sources , Fungi -- Biotechnology , Enzymes -- Biotechnology
- Language: English
- Type: Thesis , MSc , Masters
- Identifier: vital:3861 , http://hdl.handle.net/10962/d1013447
- Description: Bioethanol is likely to be a large contributor to the fuel sector of industry in the near future. Current research trends are geared towards utilizing food crops as substrate for bioethanol fermentation; however, this is the source of much controversy. Utilizing food crops for fuel purposes is anticipated to cause massive food shortages worldwide. Cellulose is the most abundant renewable resource on earth and is subject to a wide array of scientific study in order to utilize the glucose contained within it. Waste paper has a high degree of cellulose associated with it, which makes it an ideal target for cellulose biotechnology with the ultimate end goal of bioethanol production. This study focussed on producing the necessary enzymes to hydrolyse the cellulose found in waste paper and using the sugars produced to produce ethanol. The effects of various printing inks had on the production of sugars and the total envirorunental impact of the effluents produced during the production line were also examined. It was found that the fungus Trichoderma longibrachiatum DSM 769 grown in Mandel's medium with waste newspaper as the sole carbon source at 28 °C for 6 days produced extracellular cellulase enzymes with an activity of 0.203 ± 0.009 FPU.ml⁻¹, significantly higher activity as compared to other paper sources. This extracellular cellulase was used to hydrolyse waste newspaper and office paper, with office paper yielding the highest degree of sugar production with an end concentration of 5.80 ± 0.19 g/1 at 40 °C. Analysis by HPLC showed that although glucose was the major product at 4.35 ± 0.12 g/1, cellobiose was also produced in appreciable amounts (1.97 ± 0.71 g/1). The sugar solution was used as a substrate for Saccharomyces cerevisiae DSM 1333 and ethanol was produced at a level of 1.79 ± 0.26 g/1, the presence of which was confirmed by a 600 MHz NMR spectrum. It was found that cellobiose was not fermented by this strain of S. cerevisiae. Certain components of inks (the PAHs phenanthrene and naphthalene) were found to have a slight inhibitory effect (approximately 15% decrease) on the cellulase enzymes at very high concentrations (approximately 600 μg/1 in aqueous medium), while anthracene had no effect. Whole newsprint ink was shown not to sorb glucose. The environmental analysis of the effluents produced showed that in order for the effluents to be discharged into an aqueous ecosystem they would have to be diluted up to 200 times. They were also shown to have the potential to cause severe machinery damage if reused without proper treatment.
- Full Text:
- Date Issued: 2010
- Authors: Voigt, Paul George
- Date: 2010
- Subjects: Biomass energy , Cellulose -- Biodegradation , Waste paper -- Recycling , Biomass chemicals -- Economic aspects , Renewable energy sources , Fungi -- Biotechnology , Enzymes -- Biotechnology
- Language: English
- Type: Thesis , MSc , Masters
- Identifier: vital:3861 , http://hdl.handle.net/10962/d1013447
- Description: Bioethanol is likely to be a large contributor to the fuel sector of industry in the near future. Current research trends are geared towards utilizing food crops as substrate for bioethanol fermentation; however, this is the source of much controversy. Utilizing food crops for fuel purposes is anticipated to cause massive food shortages worldwide. Cellulose is the most abundant renewable resource on earth and is subject to a wide array of scientific study in order to utilize the glucose contained within it. Waste paper has a high degree of cellulose associated with it, which makes it an ideal target for cellulose biotechnology with the ultimate end goal of bioethanol production. This study focussed on producing the necessary enzymes to hydrolyse the cellulose found in waste paper and using the sugars produced to produce ethanol. The effects of various printing inks had on the production of sugars and the total envirorunental impact of the effluents produced during the production line were also examined. It was found that the fungus Trichoderma longibrachiatum DSM 769 grown in Mandel's medium with waste newspaper as the sole carbon source at 28 °C for 6 days produced extracellular cellulase enzymes with an activity of 0.203 ± 0.009 FPU.ml⁻¹, significantly higher activity as compared to other paper sources. This extracellular cellulase was used to hydrolyse waste newspaper and office paper, with office paper yielding the highest degree of sugar production with an end concentration of 5.80 ± 0.19 g/1 at 40 °C. Analysis by HPLC showed that although glucose was the major product at 4.35 ± 0.12 g/1, cellobiose was also produced in appreciable amounts (1.97 ± 0.71 g/1). The sugar solution was used as a substrate for Saccharomyces cerevisiae DSM 1333 and ethanol was produced at a level of 1.79 ± 0.26 g/1, the presence of which was confirmed by a 600 MHz NMR spectrum. It was found that cellobiose was not fermented by this strain of S. cerevisiae. Certain components of inks (the PAHs phenanthrene and naphthalene) were found to have a slight inhibitory effect (approximately 15% decrease) on the cellulase enzymes at very high concentrations (approximately 600 μg/1 in aqueous medium), while anthracene had no effect. Whole newsprint ink was shown not to sorb glucose. The environmental analysis of the effluents produced showed that in order for the effluents to be discharged into an aqueous ecosystem they would have to be diluted up to 200 times. They were also shown to have the potential to cause severe machinery damage if reused without proper treatment.
- Full Text:
- Date Issued: 2010
Messages from the deep : water divinities, dreams and diviners in Southern Africa
- Authors: Bernard, Penelope Susan
- Date: 2010
- Subjects: Dreams -- Religious aspects , Zulu (African people) -- Religion , Xhosa (African people) -- Religion , Shamanism -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2112 , http://hdl.handle.net/10962/d1007644 , Dreams -- Religious aspects , Zulu (African people) -- Religion , Xhosa (African people) -- Religion , Shamanism -- South Africa
- Description: This thesis is a comparative regional study of a complex of beliefs and practices regarding the water divinities in southern Africa. These snake and mermaid-like divinities, which are said to work in conjunction with one's ancestors, are believed to be responsible for the calling and training of certain diviner-healers by taking them underwater for periods of time. In addition to granting healing knowledge, these divinities are associated with fertility, water and rain, and the origins of humanity. The research combines comparative ethnography with the anthropology of extraordinary experience (AEE), and focuses particularly on the Zulu, Cape Nguni, Shona and Khoisan groups. The use of the 'radical participation' method, as recommended by AEE, was facilitated by the author being identified as having a ' calling' from these water divinities, which subsequently resulted in her initiation under the guidance of a Zulu isangoma (diviner-healer) who had reputedly been taken underwater. The research details the rituals that were performed and how dreams are used to guide the training process of izangoma. This resulted in the research process being largely dream-directed, in that the author traces how the izangoma responded to various dreams she had and how these responses opened new avenues for understanding the phenomenon of the water divinities. The comparative study thus combines literature sources, field research and dream-directed experiences, and reveals a complex of recurring themes, symbols and norms pertaining to the water divinities across the selected groups. In seeking to explain both the commonalities and differences between these groups, the author argues for a four-level explanatory model that combines both conventional anthropological theory and extraordinary experience. Responses to the author's dream-led experiences are used to throw light on the conflicting discourses of morality regarding traditional healers and the water divinities in the context of political-economic transformations relating to capitalism and the moral economy; to illuminate the blending of ideas and practices between Zulu Zionists and diviner-healer traditions; and to link up with certain issues relating to San rock art, rain-making and healing rituals, which contribute to the debates regarding trance-induced rock art in southern Africa.
- Full Text:
- Date Issued: 2010
- Authors: Bernard, Penelope Susan
- Date: 2010
- Subjects: Dreams -- Religious aspects , Zulu (African people) -- Religion , Xhosa (African people) -- Religion , Shamanism -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2112 , http://hdl.handle.net/10962/d1007644 , Dreams -- Religious aspects , Zulu (African people) -- Religion , Xhosa (African people) -- Religion , Shamanism -- South Africa
- Description: This thesis is a comparative regional study of a complex of beliefs and practices regarding the water divinities in southern Africa. These snake and mermaid-like divinities, which are said to work in conjunction with one's ancestors, are believed to be responsible for the calling and training of certain diviner-healers by taking them underwater for periods of time. In addition to granting healing knowledge, these divinities are associated with fertility, water and rain, and the origins of humanity. The research combines comparative ethnography with the anthropology of extraordinary experience (AEE), and focuses particularly on the Zulu, Cape Nguni, Shona and Khoisan groups. The use of the 'radical participation' method, as recommended by AEE, was facilitated by the author being identified as having a ' calling' from these water divinities, which subsequently resulted in her initiation under the guidance of a Zulu isangoma (diviner-healer) who had reputedly been taken underwater. The research details the rituals that were performed and how dreams are used to guide the training process of izangoma. This resulted in the research process being largely dream-directed, in that the author traces how the izangoma responded to various dreams she had and how these responses opened new avenues for understanding the phenomenon of the water divinities. The comparative study thus combines literature sources, field research and dream-directed experiences, and reveals a complex of recurring themes, symbols and norms pertaining to the water divinities across the selected groups. In seeking to explain both the commonalities and differences between these groups, the author argues for a four-level explanatory model that combines both conventional anthropological theory and extraordinary experience. Responses to the author's dream-led experiences are used to throw light on the conflicting discourses of morality regarding traditional healers and the water divinities in the context of political-economic transformations relating to capitalism and the moral economy; to illuminate the blending of ideas and practices between Zulu Zionists and diviner-healer traditions; and to link up with certain issues relating to San rock art, rain-making and healing rituals, which contribute to the debates regarding trance-induced rock art in southern Africa.
- Full Text:
- Date Issued: 2010
Time course of performance changes and fatigue markers during training for the ironman triathlon
- Authors: Joiner, Alexander Jason
- Date: 2010
- Subjects: Ironman triathlons -- Training , Physical education and training -- Physiological aspects , Endurance sports -- Training , Sports -- Physiological aspects , Fatigue
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5133 , http://hdl.handle.net/10962/d1005212 , Ironman triathlons -- Training , Physical education and training -- Physiological aspects , Endurance sports -- Training , Sports -- Physiological aspects , Fatigue
- Description:
Suboptimal preparation for the Ironman triathlon can have detrimental effects on mental and physical condition. The purpose of this longitudinal investigation was to examine the relationship between a number of performance changes and fatigue markers during training for an Ironman as well as immediately after the event, in an attempt to better understand the effects of ultraendurance training. Eighteen athletes training for the Ironman; South Africa, 2009 were recruited for the study. Over the 6 month data collection period body mass, training load (TRIMP and Session x RPE methods), physiological responses (waking heart rate, postural dizziness, sleep ratings), changes in psychological state (profile of mood states - POMS), reported immunological responses (symptoms of illness), biochemical changes (salivary cortisol and alpha amylase) and performance (8 km submaximal running time trial (TT) and race day performance) were measured. These responses were compared to a control sample (n=15). Results show a significant increase (p<0.05) in training load (3899.4 ± 2517.8) four weeks prior to the event. Fatigue scores significantly increased (p<0.05) concurrently with this significant increase (p<0.05) in training. TT performance did not significantly (p<0.05) alter during the time course of training. It was however strongly correlated to training load (R2=0.85) and modestly related to race performance (R2=0.65). The signs and symptoms of upper respiratory tract infections (URTI) were prevalent during the training period, decreasing during the taper and race period. Large standard deviations were found within the majority of the responses. During the final two weeks of preparation, tension scores were significantly increased (p<0.05) while training load significantly decreased (p<0.05) during the final week of preparation. Cortisol increased significantly (p<0.05) immediately post race (0.507±0.15
- Full Text:
- Date Issued: 2010
- Authors: Joiner, Alexander Jason
- Date: 2010
- Subjects: Ironman triathlons -- Training , Physical education and training -- Physiological aspects , Endurance sports -- Training , Sports -- Physiological aspects , Fatigue
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5133 , http://hdl.handle.net/10962/d1005212 , Ironman triathlons -- Training , Physical education and training -- Physiological aspects , Endurance sports -- Training , Sports -- Physiological aspects , Fatigue
- Description:
Suboptimal preparation for the Ironman triathlon can have detrimental effects on mental and physical condition. The purpose of this longitudinal investigation was to examine the relationship between a number of performance changes and fatigue markers during training for an Ironman as well as immediately after the event, in an attempt to better understand the effects of ultraendurance training. Eighteen athletes training for the Ironman; South Africa, 2009 were recruited for the study. Over the 6 month data collection period body mass, training load (TRIMP and Session x RPE methods), physiological responses (waking heart rate, postural dizziness, sleep ratings), changes in psychological state (profile of mood states - POMS), reported immunological responses (symptoms of illness), biochemical changes (salivary cortisol and alpha amylase) and performance (8 km submaximal running time trial (TT) and race day performance) were measured. These responses were compared to a control sample (n=15). Results show a significant increase (p<0.05) in training load (3899.4 ± 2517.8) four weeks prior to the event. Fatigue scores significantly increased (p<0.05) concurrently with this significant increase (p<0.05) in training. TT performance did not significantly (p<0.05) alter during the time course of training. It was however strongly correlated to training load (R2=0.85) and modestly related to race performance (R2=0.65). The signs and symptoms of upper respiratory tract infections (URTI) were prevalent during the training period, decreasing during the taper and race period. Large standard deviations were found within the majority of the responses. During the final two weeks of preparation, tension scores were significantly increased (p<0.05) while training load significantly decreased (p<0.05) during the final week of preparation. Cortisol increased significantly (p<0.05) immediately post race (0.507±0.15
- Full Text:
- Date Issued: 2010
A critical analysis of discourses constructing portfolio assessment practices in three Eastern Cape schools
- Authors: Jones, Barbara Ellen
- Date: 2010
- Subjects: Discourse analysis -- South Africa -- Eastern Cape -- Case studies Competency-based education -- South Africa -- Eastern Cape -- Case studies Educational tests and measurements -- South Africa -- Eastern Cape -- Case studies Education -- Evaluation -- South Africa -- Eastern Cape -- Case studies Curriculum evaluation -- South Africa -- Eastern Cape -- Case studies
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1468 , http://hdl.handle.net/10962/d1003349
- Description: In 1998, South Africa introduced outcomes-based education to affect the shifts in pedagogy considered necessary following the move to democracy in 1994. Across the globe, the use of the portfolio to assess learners is increasingly being promoted as a form of progressive pedagogy. Hence, its adoption by the country as a new form of assessment practice was warranted. However, how the portfolio is constructed and perceived by educators within the classroom can become problematic in practice. This was apparent in the South African context and justifies research into how the portfolio is constructed as an assessment method in educational policy and by educators in the classrooms. The Curriculum Guide Directive text and the transcripts of twenty-one interviews carried out in three Eastern Cape schools served as the source of data for this study. Discourses make up a powerful framework of spoken, written and symbolic texts of institutional bureaucracies. Within these institutions human subjects are defined and constructed. Therefore, discourse construction acts as institutional forms of knowledge which can exude power over the individual if not made transparent. I used critical discourse analysis to uncover the discourses that were embedded within the Curriculum Guide Directive and to identify the discourses entrenched within the educators’ perceptions of the portfolio. The discourses in the Curriculum Guide Directive suggest the construction of the portfolio as a method of surveillance to track whether the educators are preparing learners for the school leaving examination and instructing the educators to provide evidence in the form of a portfolio. Analysis of the transcripts of the twenty one interviews with educators from three schools in the Eastern Cape indicated that the portfolio was not seen as a form of assessment by the educators, but as a form of discipline put in place by the Department of Education to ensure that they are preparing the learners for the school leaving examination and are able to produce proof of this preparation in the form of a portfolio. The discourses from the research highlighted that the school leaving examination is hegemonised into South African educational pedagogy as a form of traditional assessment and is not challenged even when it is no longer bringing about the dominant ideological goals. The portfolio was not seen as an assessment method by the educators nor represented as such in the Curriculum Guide Directive. Therefore, the job of the portfolio became that of a policing tool. Thus, a school curriculum which has been introduced with the intent of furthering social justice can become exclusive in practice, even with the best intentions.
- Full Text:
- Date Issued: 2010
- Authors: Jones, Barbara Ellen
- Date: 2010
- Subjects: Discourse analysis -- South Africa -- Eastern Cape -- Case studies Competency-based education -- South Africa -- Eastern Cape -- Case studies Educational tests and measurements -- South Africa -- Eastern Cape -- Case studies Education -- Evaluation -- South Africa -- Eastern Cape -- Case studies Curriculum evaluation -- South Africa -- Eastern Cape -- Case studies
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1468 , http://hdl.handle.net/10962/d1003349
- Description: In 1998, South Africa introduced outcomes-based education to affect the shifts in pedagogy considered necessary following the move to democracy in 1994. Across the globe, the use of the portfolio to assess learners is increasingly being promoted as a form of progressive pedagogy. Hence, its adoption by the country as a new form of assessment practice was warranted. However, how the portfolio is constructed and perceived by educators within the classroom can become problematic in practice. This was apparent in the South African context and justifies research into how the portfolio is constructed as an assessment method in educational policy and by educators in the classrooms. The Curriculum Guide Directive text and the transcripts of twenty-one interviews carried out in three Eastern Cape schools served as the source of data for this study. Discourses make up a powerful framework of spoken, written and symbolic texts of institutional bureaucracies. Within these institutions human subjects are defined and constructed. Therefore, discourse construction acts as institutional forms of knowledge which can exude power over the individual if not made transparent. I used critical discourse analysis to uncover the discourses that were embedded within the Curriculum Guide Directive and to identify the discourses entrenched within the educators’ perceptions of the portfolio. The discourses in the Curriculum Guide Directive suggest the construction of the portfolio as a method of surveillance to track whether the educators are preparing learners for the school leaving examination and instructing the educators to provide evidence in the form of a portfolio. Analysis of the transcripts of the twenty one interviews with educators from three schools in the Eastern Cape indicated that the portfolio was not seen as a form of assessment by the educators, but as a form of discipline put in place by the Department of Education to ensure that they are preparing the learners for the school leaving examination and are able to produce proof of this preparation in the form of a portfolio. The discourses from the research highlighted that the school leaving examination is hegemonised into South African educational pedagogy as a form of traditional assessment and is not challenged even when it is no longer bringing about the dominant ideological goals. The portfolio was not seen as an assessment method by the educators nor represented as such in the Curriculum Guide Directive. Therefore, the job of the portfolio became that of a policing tool. Thus, a school curriculum which has been introduced with the intent of furthering social justice can become exclusive in practice, even with the best intentions.
- Full Text:
- Date Issued: 2010
The identification and evaluation of key sustainable development indicators and the development of a conceptual decision-making model for capital investment within Gold Fields Limited (GFL)
- Authors: Jacobs, Phillip A H
- Date: 2010
- Subjects: Industrial management -- Environmental aspects -- South Africa -- Case studies Social responsibility of business -- South Africa -- Case studies Environmental protection -- South Africa -- Case studies Sustainable development -- South Africa -- Case studies Business ethics -- South Africa -- Case studies Industries -- Social aspects -- South Africa -- Case studies Industries -- Moral and ethical aspects -- South Africa -- Case studies Gold Fields Ltd -- Case studies
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:820 , http://hdl.handle.net/10962/d1008304
- Description: The current trends in sustainable development (SO) were examined in this study, which brought about the realisation that SO has become a business imperative. Mining, which is a highly impacting industry, is faced with the dilemma of implementing the principles of SO despite the realisation that its activities are severely limited by· the finite nature of the resource it is capitalising on. This reality, however, does not detract from the non-negotiable requirement for the industry to meet the increasing pressures to act responsibly towards the environment and the community in which it operates. Gold Fields has stepped up to the plate and has already taken several steps to achieve this end. These include the adoption of SO in its Vision, Values and strategies and the development and implementation of a SO framework to ensure the integration of the principles of SO into the business. Furthermore, Gold Fields has also entered into voluntary activities that further cement the commitment the company has towards so. These other initiatives include, inter alia, its International Council on Mining and Metals membership, UN Global Compact participation, becoming a signatory to the cyanide code, IS014001, and so on. This study focussed on several indicator categories and the identification of a set of supporting sustainable development indicators (SOls) for each, which included environmental, social, economic, technological, and ethics, legal and corporate governance (not in order of priority). These indicators were assessed by a carefully selected group of respondents whose collective wisdom and expertise were used to identify and weight supporting SOls for each of the indicator categories. These supporting SOls were in turn used to develop a model that is able to assist in the business's decision making processes when capital investment is being considered . A water treatment project that is currently being considered by Gold Fields was utilised to demonstrate how the decision making model can be applied to two different scenarios. The result clearly and successfully demonstrated that by proactively taking environmental, economic, social, technological, and ethics, legal and corporate governance considerations into account, a gold mining company is able to increase the level of SO of a capital investment project.
- Full Text:
- Date Issued: 2010
- Authors: Jacobs, Phillip A H
- Date: 2010
- Subjects: Industrial management -- Environmental aspects -- South Africa -- Case studies Social responsibility of business -- South Africa -- Case studies Environmental protection -- South Africa -- Case studies Sustainable development -- South Africa -- Case studies Business ethics -- South Africa -- Case studies Industries -- Social aspects -- South Africa -- Case studies Industries -- Moral and ethical aspects -- South Africa -- Case studies Gold Fields Ltd -- Case studies
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:820 , http://hdl.handle.net/10962/d1008304
- Description: The current trends in sustainable development (SO) were examined in this study, which brought about the realisation that SO has become a business imperative. Mining, which is a highly impacting industry, is faced with the dilemma of implementing the principles of SO despite the realisation that its activities are severely limited by· the finite nature of the resource it is capitalising on. This reality, however, does not detract from the non-negotiable requirement for the industry to meet the increasing pressures to act responsibly towards the environment and the community in which it operates. Gold Fields has stepped up to the plate and has already taken several steps to achieve this end. These include the adoption of SO in its Vision, Values and strategies and the development and implementation of a SO framework to ensure the integration of the principles of SO into the business. Furthermore, Gold Fields has also entered into voluntary activities that further cement the commitment the company has towards so. These other initiatives include, inter alia, its International Council on Mining and Metals membership, UN Global Compact participation, becoming a signatory to the cyanide code, IS014001, and so on. This study focussed on several indicator categories and the identification of a set of supporting sustainable development indicators (SOls) for each, which included environmental, social, economic, technological, and ethics, legal and corporate governance (not in order of priority). These indicators were assessed by a carefully selected group of respondents whose collective wisdom and expertise were used to identify and weight supporting SOls for each of the indicator categories. These supporting SOls were in turn used to develop a model that is able to assist in the business's decision making processes when capital investment is being considered . A water treatment project that is currently being considered by Gold Fields was utilised to demonstrate how the decision making model can be applied to two different scenarios. The result clearly and successfully demonstrated that by proactively taking environmental, economic, social, technological, and ethics, legal and corporate governance considerations into account, a gold mining company is able to increase the level of SO of a capital investment project.
- Full Text:
- Date Issued: 2010
Brood cycles in queenless colonies of Apis mellifera capensis
- Authors: Crous, Kendall Lauren
- Date: 2010
- Subjects: Cape honeybee , Bee culture -- Queen rearing , Bees -- Breeding
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5652 , http://hdl.handle.net/10962/d1005335 , Cape honeybee , Bee culture -- Queen rearing , Bees -- Breeding
- Description: In a honeybee colony, the loss of a queen is considered to be a serious occurrence and, if a new queen is not produced, ultimately doomed. However, in colonies of Apis mellifera capensis (Cape honeybees), numerous pathways are available for a colony which unexpectedly losses a queen. At the onset of this experiment, four colonies of A. m. capensis were dequeened. Following this photographs of all brood frames in each colony were taken and the contents of the cells analysed. Cells were chosen at random but once selected were repeatedly analysed for the duration of the experiment. The contents of a total of 44 888 individual cells were analysed. Any queen cells constructed during the sampling period were removed, maintaining a queenless state. In each colony, as predicted, the removal of the queen evoked a variety of responses in an attempt to rectify the sudden loss. However, ultimately three of the four colonies absconded, leaving little by way of stores. Three of the four colonies initially attempted to rear a new queen while one colony was immediately invaded by a presumed foreign queen and hence any attempt at queen cell construction ceased. An increased number of queen cells in the swarm position were recorded in all colonies. The invasion of a colony by a foreign queen was considered to be a new pathway available for queenless colonies of A.m capensis. Worker policing and suspected brood cannibalism was prevalent in all sampled colonies yet in addition, the transfer of eggs and larvae from cell to cell was also observed which may have increased the suspected cases of policing and cannibalism. It was unclear whether an egg or larva had been consumed or simply moved to another cell on the brood frame. All colonies contained eggs from laying workers to varying degrees, based on the length of each individual sampling period which varied between colonies due to differences in absconding dates. A steady breakdown in the effectiveness of the division of labour amongst the worker bees was observed in each of the colonies highlighting the vital role of a queen. This breakdown was clearly seen in the reduction in general housekeeping within a colony. An increase in stores indicated a possible increase in the number of forager bees, thereby reducing the number of worker bees available for other duties. Pathways available to broodright colonies and strategies used following sudden queen loss are discussed.
- Full Text:
- Date Issued: 2010
- Authors: Crous, Kendall Lauren
- Date: 2010
- Subjects: Cape honeybee , Bee culture -- Queen rearing , Bees -- Breeding
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5652 , http://hdl.handle.net/10962/d1005335 , Cape honeybee , Bee culture -- Queen rearing , Bees -- Breeding
- Description: In a honeybee colony, the loss of a queen is considered to be a serious occurrence and, if a new queen is not produced, ultimately doomed. However, in colonies of Apis mellifera capensis (Cape honeybees), numerous pathways are available for a colony which unexpectedly losses a queen. At the onset of this experiment, four colonies of A. m. capensis were dequeened. Following this photographs of all brood frames in each colony were taken and the contents of the cells analysed. Cells were chosen at random but once selected were repeatedly analysed for the duration of the experiment. The contents of a total of 44 888 individual cells were analysed. Any queen cells constructed during the sampling period were removed, maintaining a queenless state. In each colony, as predicted, the removal of the queen evoked a variety of responses in an attempt to rectify the sudden loss. However, ultimately three of the four colonies absconded, leaving little by way of stores. Three of the four colonies initially attempted to rear a new queen while one colony was immediately invaded by a presumed foreign queen and hence any attempt at queen cell construction ceased. An increased number of queen cells in the swarm position were recorded in all colonies. The invasion of a colony by a foreign queen was considered to be a new pathway available for queenless colonies of A.m capensis. Worker policing and suspected brood cannibalism was prevalent in all sampled colonies yet in addition, the transfer of eggs and larvae from cell to cell was also observed which may have increased the suspected cases of policing and cannibalism. It was unclear whether an egg or larva had been consumed or simply moved to another cell on the brood frame. All colonies contained eggs from laying workers to varying degrees, based on the length of each individual sampling period which varied between colonies due to differences in absconding dates. A steady breakdown in the effectiveness of the division of labour amongst the worker bees was observed in each of the colonies highlighting the vital role of a queen. This breakdown was clearly seen in the reduction in general housekeeping within a colony. An increase in stores indicated a possible increase in the number of forager bees, thereby reducing the number of worker bees available for other duties. Pathways available to broodright colonies and strategies used following sudden queen loss are discussed.
- Full Text:
- Date Issued: 2010
The political economy of development aid: an investigation of three donor-funded HIV/AIDS programmes broadcast by Malawi television from 2004 to 2007
- Authors: Mulonya, Rodrick K A R
- Date: 2010
- Subjects: Economic assistance -- Malawi -- Management , Public service television programs -- Malawi , AIDS (Disease) and mass media -- Malawi , HIV infections -- Information services -- Malawi , Communication in public health -- Malawi , Economic assistance -- Social aspects -- Malawi , Malawi -- Social conditions , Mass media -- Social aspects -- Malawi , Mass media criticism -- Malawi
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3471 , http://hdl.handle.net/10962/d1002926
- Description: Development aid in most of the developing countries can sometimes compromise the principles of public service broadcasting (PSB). This may be true when reflected against the tension between donor financed programmes in Malawi and the mandate of Television Malawi (TVM). Although the donor intentions are noble, the strings attached to the funding are sometimes retrogressive to the role of PSBs. A case in point is how donors dictate terms on the HIV/Aids communication strategies at TVM. Producers receive money from donors with strings attached on how the money should be used and accounted for. If producers deviate they are sanctioned through withholding funding, shifting schedules and reducing the funding frequency. The donors also dictate who to interview on what subject, how to conduct capacity building. Some scholars have researched much on the impact of commercialisation of the media. This study is a departure from these traditional interferences; it interrogates the interest of philanthropy tendencies by international donors in the three chosen HIV/Aids programmes broadcast by TVM. The study investigates the extent of pressure exerted by donors on the producers of HIV/Aids programmes in Malawi. Thus, the study seeks to illicit specifics in the power relationship between the donor and the producer hence the study employs the political economy of development aid as applied to the public service broadcasting and communication for development. The study employed qualitative research methods and techniques (in-depth interviews, case study and document analysis). The study reveals how donor ideologies dominate the Aids messages-content output of the texts constructed. The study argues that cultural alienation of the Malawian audiences retards efforts of donors in combating HIV infection rate.
- Full Text:
- Date Issued: 2010
- Authors: Mulonya, Rodrick K A R
- Date: 2010
- Subjects: Economic assistance -- Malawi -- Management , Public service television programs -- Malawi , AIDS (Disease) and mass media -- Malawi , HIV infections -- Information services -- Malawi , Communication in public health -- Malawi , Economic assistance -- Social aspects -- Malawi , Malawi -- Social conditions , Mass media -- Social aspects -- Malawi , Mass media criticism -- Malawi
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3471 , http://hdl.handle.net/10962/d1002926
- Description: Development aid in most of the developing countries can sometimes compromise the principles of public service broadcasting (PSB). This may be true when reflected against the tension between donor financed programmes in Malawi and the mandate of Television Malawi (TVM). Although the donor intentions are noble, the strings attached to the funding are sometimes retrogressive to the role of PSBs. A case in point is how donors dictate terms on the HIV/Aids communication strategies at TVM. Producers receive money from donors with strings attached on how the money should be used and accounted for. If producers deviate they are sanctioned through withholding funding, shifting schedules and reducing the funding frequency. The donors also dictate who to interview on what subject, how to conduct capacity building. Some scholars have researched much on the impact of commercialisation of the media. This study is a departure from these traditional interferences; it interrogates the interest of philanthropy tendencies by international donors in the three chosen HIV/Aids programmes broadcast by TVM. The study investigates the extent of pressure exerted by donors on the producers of HIV/Aids programmes in Malawi. Thus, the study seeks to illicit specifics in the power relationship between the donor and the producer hence the study employs the political economy of development aid as applied to the public service broadcasting and communication for development. The study employed qualitative research methods and techniques (in-depth interviews, case study and document analysis). The study reveals how donor ideologies dominate the Aids messages-content output of the texts constructed. The study argues that cultural alienation of the Malawian audiences retards efforts of donors in combating HIV infection rate.
- Full Text:
- Date Issued: 2010
An analysis of emerging forms of social organisation and agency in the aftermath of 'fast track' land reform in Zimbabwe
- Authors: Murisa, Tendai
- Date: 2010
- Subjects: Land reform -- Zimbabwe Right of property -- Zimbabwe Zimbabwe -- Economic conditions Zimbabwe -- Social conditions -- 1980- Zimbabwe -- Politics and government -- 1980- Zimbabwe -- Social life and customs Land settlement -- Social aspects -- Zimbabwe Group identity -- Zimbabwe Social change -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3293 , http://hdl.handle.net/10962/d1003081
- Description: The fast track land reform programme resulted in a fundamental reorganisation of rural relations in Zimbabwe, changing the landscape in an irreversible way with people from diverse backgrounds converging on former white-owned farms. This thesis tells the story of how the newly resettled land beneficiaries are organising themselves socially in response to various economic challenges. It makes a contribution towards understanding how redistributive land reforms and local government restructuring influence rural social organisation and agency. Furthermore the study examines local perceptions on the meanings of the „farm‟ and „land redistribution‟. An utterance by one war veteran “what used to be your farm is now our land and you are free to take your farm but leave our land” provides an alternative rendition to contestations of restitution versus a purely farm productionist discourse. The study, through an analysis of primary and secondary data, provides a fresh understanding of the social outcomes of fast track. It traces the evolution of land and agrarian reforms in post-independence Zimbabwe and the political and social economic context that led to „fast track‟. Through an analysis of field findings the thesis is able to define the dominant social groups that were resettled during fast track and the challenges they face in utilising the land. The findings show that the majority of the land beneficiaries were from the customary areas, with limited agricultural experiences. Local cooperation within informal networks and local farmer groups has been identified as one of the ways in which social reproduction is being organised. These groups are responsible for enhancing production capacity but they face a number of constraints. The study derives its theoretical foundation from the post 1980s debates on rural society dominated by Mafeje (1993, 2003), Rahmato (1991) and Mamdani (1996). The debates centred on how institutions of inclusion, authority and cooperation such as the lineage groups, local farmer groups and traditional authority remain relevant in the organisation of post-independent rural African society especially in a context of increased commoditisation of rural relations of production. Using theoretical insights derived from analysing the role of the lineage groups in the allocation of critical resources such as land and the influence of traditional authority (indirect rule) as a form of local government, the study examines how social organisation is emerging in areas where neither lineage nor traditional authority are not dominant. The thesis of rural cooperation through local groups as advanced by Rahmato (1991) and Moyo (2002) provides partial insights into the response mechanisms that land beneficiaries invoke in this instance. It is not necessarily an autonomous space of organisation but rather the state is actively involved through various functionaries including extension officers who invariably advance a very productionist approach. The state‟s monopoly through its local functionaries hides its political cooptation effect by emphasising organisation for production without questioning the manner in which that production is externally controlled through limited rights over land, the state‟s monopoly over inputs supply and markets for commodities. Whilst land reform has been driven by local participation through land occupations, local government reform has been technocratically determined through Ministerial directives. There is however little innovation in the form of local government that is being introduced. It expands the fusion of authority between elected Rural District Councils and unelected traditional authority functionaries. The forms of social organisation and agency that have emerged remain subordinated to the state with no links to other networks of rural producers‟ associations and urban civil society organisations. These developments form part of a longheld tradition within the Zimbabwean state where the legitimacy of local organisation and authority is usurped to service the interests of the state. Thus whilst land reform has to a certain extent accommodated the majority poor, the ensuing local government and agrarian reforms are more focused on limiting their participation in broader processes of political engagement around distribution and accumulation and their own governance.
- Full Text:
- Date Issued: 2010
- Authors: Murisa, Tendai
- Date: 2010
- Subjects: Land reform -- Zimbabwe Right of property -- Zimbabwe Zimbabwe -- Economic conditions Zimbabwe -- Social conditions -- 1980- Zimbabwe -- Politics and government -- 1980- Zimbabwe -- Social life and customs Land settlement -- Social aspects -- Zimbabwe Group identity -- Zimbabwe Social change -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3293 , http://hdl.handle.net/10962/d1003081
- Description: The fast track land reform programme resulted in a fundamental reorganisation of rural relations in Zimbabwe, changing the landscape in an irreversible way with people from diverse backgrounds converging on former white-owned farms. This thesis tells the story of how the newly resettled land beneficiaries are organising themselves socially in response to various economic challenges. It makes a contribution towards understanding how redistributive land reforms and local government restructuring influence rural social organisation and agency. Furthermore the study examines local perceptions on the meanings of the „farm‟ and „land redistribution‟. An utterance by one war veteran “what used to be your farm is now our land and you are free to take your farm but leave our land” provides an alternative rendition to contestations of restitution versus a purely farm productionist discourse. The study, through an analysis of primary and secondary data, provides a fresh understanding of the social outcomes of fast track. It traces the evolution of land and agrarian reforms in post-independence Zimbabwe and the political and social economic context that led to „fast track‟. Through an analysis of field findings the thesis is able to define the dominant social groups that were resettled during fast track and the challenges they face in utilising the land. The findings show that the majority of the land beneficiaries were from the customary areas, with limited agricultural experiences. Local cooperation within informal networks and local farmer groups has been identified as one of the ways in which social reproduction is being organised. These groups are responsible for enhancing production capacity but they face a number of constraints. The study derives its theoretical foundation from the post 1980s debates on rural society dominated by Mafeje (1993, 2003), Rahmato (1991) and Mamdani (1996). The debates centred on how institutions of inclusion, authority and cooperation such as the lineage groups, local farmer groups and traditional authority remain relevant in the organisation of post-independent rural African society especially in a context of increased commoditisation of rural relations of production. Using theoretical insights derived from analysing the role of the lineage groups in the allocation of critical resources such as land and the influence of traditional authority (indirect rule) as a form of local government, the study examines how social organisation is emerging in areas where neither lineage nor traditional authority are not dominant. The thesis of rural cooperation through local groups as advanced by Rahmato (1991) and Moyo (2002) provides partial insights into the response mechanisms that land beneficiaries invoke in this instance. It is not necessarily an autonomous space of organisation but rather the state is actively involved through various functionaries including extension officers who invariably advance a very productionist approach. The state‟s monopoly through its local functionaries hides its political cooptation effect by emphasising organisation for production without questioning the manner in which that production is externally controlled through limited rights over land, the state‟s monopoly over inputs supply and markets for commodities. Whilst land reform has been driven by local participation through land occupations, local government reform has been technocratically determined through Ministerial directives. There is however little innovation in the form of local government that is being introduced. It expands the fusion of authority between elected Rural District Councils and unelected traditional authority functionaries. The forms of social organisation and agency that have emerged remain subordinated to the state with no links to other networks of rural producers‟ associations and urban civil society organisations. These developments form part of a longheld tradition within the Zimbabwean state where the legitimacy of local organisation and authority is usurped to service the interests of the state. Thus whilst land reform has to a certain extent accommodated the majority poor, the ensuing local government and agrarian reforms are more focused on limiting their participation in broader processes of political engagement around distribution and accumulation and their own governance.
- Full Text:
- Date Issued: 2010
Revolt as a strategy of de-reification in contemporary performance practice
- Authors: Smit, Sonja
- Date: 2010
- Subjects: Mantero, Vera Bouwer, Jaco, 1973- Performance art -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2147 , http://hdl.handle.net/10962/d1002379
- Description: This thesis investigates the concepts of revolt and de-reification and how these can be perceived and implemented within the context of performance. The argument focuses on the ability of revolt to question and unsettle processes of reification which in turn manifest a strategy of de-reification. I investigate the potential in contemporary performance practice to challenge prevailing modes of perception and restore the production of desire to the spectator through strategies of de-reification. This research is approached through a qualitative process which entails a reading and application of critical texts to the analysis. This reading/application is engaged in a dialogue with the interpretative and experiential aspects of the two works selected for analysis. Chapter One functions as an introduction to the concept of reification and the necessity of a process geared towards de-reification through revolt. I argue that revolt is already embedded in avant-garde artistic practices through an experimental and questioning approach to artistic practice and the production of meaning. Chapter Two is an analysis of Vera Mantero’s solo work, one mysterious Thing said e.e cummings* (1996). This is structured around two identifiable elements, one being the resistance to signification (designification), which is argued as a strategy of revolt within the piece. The second is the notion of abjection, which works doubly to aid the resistance to signification as well as working as a strategy of revolt by its implication in the work. Chapter Three analyses Jaco Bouwer’s Untitled (2008), specifically dealing with the notion of absence as a strategy of revolt and de-reification. The discussion is focused on the potential complication of desire through absence as enacting a larger project of revolt. As in Chapter Two, this is similarly related to the fragmentation of signs through designification which emphasises the strategy of absence. This thesis concludes with the idea that meaning-making in performance can be considered a process. Instead, the lack or failure of meaning within these selected performance practices enables a return to the individual (performer and spectator) as the agent of desire.
- Full Text:
- Date Issued: 2010
- Authors: Smit, Sonja
- Date: 2010
- Subjects: Mantero, Vera Bouwer, Jaco, 1973- Performance art -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2147 , http://hdl.handle.net/10962/d1002379
- Description: This thesis investigates the concepts of revolt and de-reification and how these can be perceived and implemented within the context of performance. The argument focuses on the ability of revolt to question and unsettle processes of reification which in turn manifest a strategy of de-reification. I investigate the potential in contemporary performance practice to challenge prevailing modes of perception and restore the production of desire to the spectator through strategies of de-reification. This research is approached through a qualitative process which entails a reading and application of critical texts to the analysis. This reading/application is engaged in a dialogue with the interpretative and experiential aspects of the two works selected for analysis. Chapter One functions as an introduction to the concept of reification and the necessity of a process geared towards de-reification through revolt. I argue that revolt is already embedded in avant-garde artistic practices through an experimental and questioning approach to artistic practice and the production of meaning. Chapter Two is an analysis of Vera Mantero’s solo work, one mysterious Thing said e.e cummings* (1996). This is structured around two identifiable elements, one being the resistance to signification (designification), which is argued as a strategy of revolt within the piece. The second is the notion of abjection, which works doubly to aid the resistance to signification as well as working as a strategy of revolt by its implication in the work. Chapter Three analyses Jaco Bouwer’s Untitled (2008), specifically dealing with the notion of absence as a strategy of revolt and de-reification. The discussion is focused on the potential complication of desire through absence as enacting a larger project of revolt. As in Chapter Two, this is similarly related to the fragmentation of signs through designification which emphasises the strategy of absence. This thesis concludes with the idea that meaning-making in performance can be considered a process. Instead, the lack or failure of meaning within these selected performance practices enables a return to the individual (performer and spectator) as the agent of desire.
- Full Text:
- Date Issued: 2010
Isolation of xylanolytic multi-enzyme complexes from Bacillus subtilis SJ01
- Authors: Jones, Sarah Melissa Jane
- Date: 2010
- Subjects: Bacillus subtilis , Xylans , Multienzyme complexes , Botanical chemistry , Cellulose , Hemicellulose , Polysaccharides
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3974 , http://hdl.handle.net/10962/d1004033 , Bacillus subtilis , Xylans , Multienzyme complexes , Botanical chemistry , Cellulose , Hemicellulose , Polysaccharides
- Description: Cellulose and hemicellulose account for a large portion of the world‘s plant biomass. In nature, these polysaccharides are intertwined forming complex materials that require multiple enzymes to degrade them. Multi-enzyme complexes (MECs) consist of a number of enzymes working in close proximity and synergistically to degrade complex substrates with higher efficiency than individual enzymes. The cellulosome is a cellulolytic MEC produced by anaerobic bacteria that has been studied extensively since its discovery in 1983. The aim of this study was to purify a cellulolytic and/or hemicellulolytic MEC from an aerobic bacterium of the Bacillus genus. Several bacterial isolates were identified using morphological characteristics and 16S rDNA sequencing, and screened for their ability to degrade cellulose and xylan using a MEC. The isolate that produced a high molecular weight protein fraction with the greatest ability to degrade Avicel®, carboxymethyl cellulose (CMC) and birchwood xylan was identified as Bacillus subtilis SJ01. An optimised growth medium, consisting of vitamins, trace elements, birchwood xylan (as the carbon source), and yeast and ammonium sulphate (as the nitrogen sources), increased the production of CMCase and xylanase enzymes from this bacterium. The removal of a competing bacterial strain from the culture and the inhibition of proteases also increased enzyme activities. A growth curve of B. subtilis SJ01 indicated that xylanase production was highest in early stationary growth phase and thus 84 hours was chosen as the best cell harvesting time. To purify the MECs produced by B. subtilis SJ01 size-exclusion chromatography on a Sephacryl S-400 column was used. It was concluded that (for the purposes of this study) the best method of concentrating the culture supernatant prior to loading onto Sephacryl S-400 was the use of ultrafiltration with a 50 kDa cut-off membrane. Two MECs, named C1 and C2 of 371 and 267 kDa, respectively, were purified from the culture supernatant of B. subtilis SJ01. Electrophoretic analysis revealed that these MECs consisted of 16 and 18 subunits, respectively, 4 of which degraded birchwood xylan and 5 of which degraded oat spelt xylan. The MECs degraded xylan substrates (C1: 0.24 U/mg, C2: 0.14 U/mg birchwood xylan) with higher efficiency than cellulose substrates (C1: 0.002 U/mg, C2: 0.01 U/mg CMC), and could therefore be considered xylanosomes. Interestingly, the MECs did not bind to insoluble birchwood xylan or Avicel® and did not contain glycosylated proteins, which are common features of cellulosomes. This study is, therefore, important in revealing the presence of MECs that differ from the cellulosome and that may have particular application in industries requiring high xylanase activity, such as the paper and pulp industry. The abundant genetic information available on B. subtilis means that this organism could also be used for genetic engineering of cellulolytic/hemicellulolytic MECs.
- Full Text:
- Date Issued: 2010
- Authors: Jones, Sarah Melissa Jane
- Date: 2010
- Subjects: Bacillus subtilis , Xylans , Multienzyme complexes , Botanical chemistry , Cellulose , Hemicellulose , Polysaccharides
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3974 , http://hdl.handle.net/10962/d1004033 , Bacillus subtilis , Xylans , Multienzyme complexes , Botanical chemistry , Cellulose , Hemicellulose , Polysaccharides
- Description: Cellulose and hemicellulose account for a large portion of the world‘s plant biomass. In nature, these polysaccharides are intertwined forming complex materials that require multiple enzymes to degrade them. Multi-enzyme complexes (MECs) consist of a number of enzymes working in close proximity and synergistically to degrade complex substrates with higher efficiency than individual enzymes. The cellulosome is a cellulolytic MEC produced by anaerobic bacteria that has been studied extensively since its discovery in 1983. The aim of this study was to purify a cellulolytic and/or hemicellulolytic MEC from an aerobic bacterium of the Bacillus genus. Several bacterial isolates were identified using morphological characteristics and 16S rDNA sequencing, and screened for their ability to degrade cellulose and xylan using a MEC. The isolate that produced a high molecular weight protein fraction with the greatest ability to degrade Avicel®, carboxymethyl cellulose (CMC) and birchwood xylan was identified as Bacillus subtilis SJ01. An optimised growth medium, consisting of vitamins, trace elements, birchwood xylan (as the carbon source), and yeast and ammonium sulphate (as the nitrogen sources), increased the production of CMCase and xylanase enzymes from this bacterium. The removal of a competing bacterial strain from the culture and the inhibition of proteases also increased enzyme activities. A growth curve of B. subtilis SJ01 indicated that xylanase production was highest in early stationary growth phase and thus 84 hours was chosen as the best cell harvesting time. To purify the MECs produced by B. subtilis SJ01 size-exclusion chromatography on a Sephacryl S-400 column was used. It was concluded that (for the purposes of this study) the best method of concentrating the culture supernatant prior to loading onto Sephacryl S-400 was the use of ultrafiltration with a 50 kDa cut-off membrane. Two MECs, named C1 and C2 of 371 and 267 kDa, respectively, were purified from the culture supernatant of B. subtilis SJ01. Electrophoretic analysis revealed that these MECs consisted of 16 and 18 subunits, respectively, 4 of which degraded birchwood xylan and 5 of which degraded oat spelt xylan. The MECs degraded xylan substrates (C1: 0.24 U/mg, C2: 0.14 U/mg birchwood xylan) with higher efficiency than cellulose substrates (C1: 0.002 U/mg, C2: 0.01 U/mg CMC), and could therefore be considered xylanosomes. Interestingly, the MECs did not bind to insoluble birchwood xylan or Avicel® and did not contain glycosylated proteins, which are common features of cellulosomes. This study is, therefore, important in revealing the presence of MECs that differ from the cellulosome and that may have particular application in industries requiring high xylanase activity, such as the paper and pulp industry. The abundant genetic information available on B. subtilis means that this organism could also be used for genetic engineering of cellulolytic/hemicellulolytic MECs.
- Full Text:
- Date Issued: 2010
The use of response surface methodology and artificial neural networks for the establishment of a design space for a sustained release salbutamol sulphate formulation
- Authors: Chaibva, Faith Anesu
- Date: 2010
- Subjects: Salbutamol sulphate Artificial intelligence -- Medical applications Neural networks (Computer science) Response surfaces (Statistics) Pharmaceutical biotechnology -- Quality contro Drugs -- Design Pharmacokinetics Drugs -- Dosage forms Drugs -- Controlled release
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3845 , http://hdl.handle.net/10962/d1010432
- Description: Quality by Design (QbD) is a systematic approach that has been recommended as suitable for the development of quality pharmaceutical products. The QbD approach commences with the definition of a quality target drug profile and predetermined objectives that are then used to direct the formulation development process with an emphasis on understanding the pharmaceutical science and manufacturing principles that apply to a product. The design space is directly linked to the use of QbD for formulation development and is a multidimensional combination and interaction of input variables and process parameters that have been demonstrated to provide an assurance of quality. The objective of these studies was to apply the principles of QbD as a framework for the optimisation of a sustained release (SR) formulation of salbutamol sulphate (SBS), and for the establishment of a design space using Response Surface Methodology (RSM) and Artificial Neural Networks (ANN). SBS is a short-acting ♭₂ agonist that is used for the management of asthma and chronic obstructive pulmonary disease (COPD). The use of a SR formulation of SBS may provide clinical benefits in the management of these respiratory disorders. Ashtalin®8 ER (Cipla Ltd., Mumbai, Maharashtra, India) was selected as a reference formulation for use in these studies. An Ishikawa or Cause and Effect diagram was used to determine the impact of formulation and process factors that have the potential to affect product quality. Key areas of concern that must be monitored include the raw materials, the manufacturing equipment and processes, and the analytical and assessment methods employed. The conditions in the laboratory and manufacturing processes were carefully monitored and recorded for any deviation from protocol, and equipment for assessment of dosage form performance, including dissolution equipment, balances and hardness testers, underwent regular maintenance. Preliminary studies to assess the potential utility of Methocel® Kl OOM, alone and in combination with other matrix forming polymers, revealed that the combination of this polymer with xanthan gum and Carbopol® has the potential to modulate the release of SBS at a specific rate, for a period of 12 hr. A central composite design using Methocel® KlOOM, xanthan gum, Carbopol® 974P and Surelease® as the granulating fluid was constructed to fully evaluate the impact of these formulation variables on the rate and extent of SBS release from manufactured formulations. The results revealed that although Methocel® KlOOM and xanthan gum had the greatest retardant effect on drug release, interactions between the polymers used in the study were also important determinants of the measureable responses. An ANN model was trained for optimisation using the data generated from a central composite study. The efficiency of the network was optimised by assessing the impact of the number of nodes in the hidden layer using a three layer Multi Layer Perceptron (MLP). The results revealed that a network with nine nodes in the hidden layer had the best predictive ability, suitable for application to formulation optimisation studies. Pharmaceutical optimisation was conducted using both the RSM and the trained ANN models. The results from the two optimisation procedures yielded two different formulation compositions that were subjected to in vitro dissolution testing using USP Apparatus 3. The results revealed that, although the formulation compositions that were derived from the optimisation procedures were different, both solutions gave reproducible results for which the dissolution profiles were indeed similar to that of the reference formulation. RSM and ANN were further investigated as possible means of establishing a design space for formulation compositions that would result in dosage forms that have similar in vitro release test profiles comparable to the reference product. Constraint plots were used to determine the bounds of the formulation variables that would result in the manufacture of dosage forms with the desired release profile. ANN simulations with hypothetical formulations that were generated within a small region of the experimental domain were investigated as a means of understanding the impact of varying the composition of the formulation on resultant dissolution profiles. Although both methods were suitable for the establishment of a design space, the use of ANN may be better suited for this purpose because of the manner in which ANN handles data. As more information about the behaviour of a formulation and its processes is generated during the product Iifecycle, ANN may be used to evaluate the impact of formulation and process variables on measureable responses. It is recommended that ANN may be suitable for the optimisation of pharmaceutical formulations and establishment of a design space in line with ICH Pharmaceutical Development [1], Quality Risk Management [2] and Pharmaceutical Quality Systems [3]
- Full Text:
- Date Issued: 2010
- Authors: Chaibva, Faith Anesu
- Date: 2010
- Subjects: Salbutamol sulphate Artificial intelligence -- Medical applications Neural networks (Computer science) Response surfaces (Statistics) Pharmaceutical biotechnology -- Quality contro Drugs -- Design Pharmacokinetics Drugs -- Dosage forms Drugs -- Controlled release
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3845 , http://hdl.handle.net/10962/d1010432
- Description: Quality by Design (QbD) is a systematic approach that has been recommended as suitable for the development of quality pharmaceutical products. The QbD approach commences with the definition of a quality target drug profile and predetermined objectives that are then used to direct the formulation development process with an emphasis on understanding the pharmaceutical science and manufacturing principles that apply to a product. The design space is directly linked to the use of QbD for formulation development and is a multidimensional combination and interaction of input variables and process parameters that have been demonstrated to provide an assurance of quality. The objective of these studies was to apply the principles of QbD as a framework for the optimisation of a sustained release (SR) formulation of salbutamol sulphate (SBS), and for the establishment of a design space using Response Surface Methodology (RSM) and Artificial Neural Networks (ANN). SBS is a short-acting ♭₂ agonist that is used for the management of asthma and chronic obstructive pulmonary disease (COPD). The use of a SR formulation of SBS may provide clinical benefits in the management of these respiratory disorders. Ashtalin®8 ER (Cipla Ltd., Mumbai, Maharashtra, India) was selected as a reference formulation for use in these studies. An Ishikawa or Cause and Effect diagram was used to determine the impact of formulation and process factors that have the potential to affect product quality. Key areas of concern that must be monitored include the raw materials, the manufacturing equipment and processes, and the analytical and assessment methods employed. The conditions in the laboratory and manufacturing processes were carefully monitored and recorded for any deviation from protocol, and equipment for assessment of dosage form performance, including dissolution equipment, balances and hardness testers, underwent regular maintenance. Preliminary studies to assess the potential utility of Methocel® Kl OOM, alone and in combination with other matrix forming polymers, revealed that the combination of this polymer with xanthan gum and Carbopol® has the potential to modulate the release of SBS at a specific rate, for a period of 12 hr. A central composite design using Methocel® KlOOM, xanthan gum, Carbopol® 974P and Surelease® as the granulating fluid was constructed to fully evaluate the impact of these formulation variables on the rate and extent of SBS release from manufactured formulations. The results revealed that although Methocel® KlOOM and xanthan gum had the greatest retardant effect on drug release, interactions between the polymers used in the study were also important determinants of the measureable responses. An ANN model was trained for optimisation using the data generated from a central composite study. The efficiency of the network was optimised by assessing the impact of the number of nodes in the hidden layer using a three layer Multi Layer Perceptron (MLP). The results revealed that a network with nine nodes in the hidden layer had the best predictive ability, suitable for application to formulation optimisation studies. Pharmaceutical optimisation was conducted using both the RSM and the trained ANN models. The results from the two optimisation procedures yielded two different formulation compositions that were subjected to in vitro dissolution testing using USP Apparatus 3. The results revealed that, although the formulation compositions that were derived from the optimisation procedures were different, both solutions gave reproducible results for which the dissolution profiles were indeed similar to that of the reference formulation. RSM and ANN were further investigated as possible means of establishing a design space for formulation compositions that would result in dosage forms that have similar in vitro release test profiles comparable to the reference product. Constraint plots were used to determine the bounds of the formulation variables that would result in the manufacture of dosage forms with the desired release profile. ANN simulations with hypothetical formulations that were generated within a small region of the experimental domain were investigated as a means of understanding the impact of varying the composition of the formulation on resultant dissolution profiles. Although both methods were suitable for the establishment of a design space, the use of ANN may be better suited for this purpose because of the manner in which ANN handles data. As more information about the behaviour of a formulation and its processes is generated during the product Iifecycle, ANN may be used to evaluate the impact of formulation and process variables on measureable responses. It is recommended that ANN may be suitable for the optimisation of pharmaceutical formulations and establishment of a design space in line with ICH Pharmaceutical Development [1], Quality Risk Management [2] and Pharmaceutical Quality Systems [3]
- Full Text:
- Date Issued: 2010
Analysis of the anti-cancer activity of novel indigenous algal compounds in breast cancer: towards the development of a model for screening anti-cancer stem cell activity
- Authors: Lawson, Jessica Clair
- Date: 2010
- Subjects: Breast -- Cancer , Breast -- Cancer -- Chemotherapy , Breast -- Cancer -- Treatment , Red algae , Brown algae , Algae -- Biotechnology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3925 , http://hdl.handle.net/10962/d1003984 , Breast -- Cancer , Breast -- Cancer -- Chemotherapy , Breast -- Cancer -- Treatment , Red algae , Brown algae , Algae -- Biotechnology
- Description: Breast cancer, the most common malignancy diagnosed in women, is one of the leading causes of death in women worldwide. In South Africa only 32% of women diagnosed with advanced breast cancer survive more than five years. The search for new chemotherapeutic agents capable of effectively treating breast cancer is therefore essential. Recent evidence supporting the cancer stem cell theory of cancer development for breast cancer challenges the current theories of cancer development and hence treatment. Cancer stem cells are a small subpopulation of tumour cells that possess properties of both cancer cells and stem cells and are believed to be the tumour-initiating population of many cancers. Cancer stem cells are inherently resistant to many chemotherapeutic agents and in this way have been associated with repopulation of tumours after chemotherapy. This phenomenon is proposed as a possible mechanism for cancer relapse after treatment. Cancer stem cells have also been implicated in metastasis, the major cause of mortality in cancer patients. Therefore, any treatment that is capable of targeting and removing breast cancer stem cells may have the theoretical potential to effectively treat breast cancer. However, there are currently no such treatments available for clinical use. We were provided access to a library of novel indigenous small molecules isolated from red and brown algae found off the Eastern Cape of South Africa. The aim of this project was to analyse the anti-cancer and anti-cancer stem cell properties of the compounds in this library and to identify „hit‟ compounds which could form the basis for future development into new anti-cancer drugs. Ten novel compounds of algal origin were tested for cytotoxicity, by determining their ability to inhibit the growth of MCF12A breast epithelial cells and MCF7 breast cancer cells using the colorimetric MTT [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] cell proliferation assay. All but one of the compounds tested exhibited cytotoxicity towards the MCF7 cancer cell line, with IC50 values (the concentration of the compound that leads to a 50% inhibition in cell growth) of between 3 μM and 90 μM. The chemotherapeutic drug paclitaxel was used as a positive control. Four of the compounds (RUMB-001, RUMB-002, RUMB-007 and RUMB-010/saragaquinoic acid) were significantly more toxic to the MCF7 cancer cell line, than the „normal‟ MCF12A breast cells and were selected as priority compounds for further analyses. In addition, two other compounds were selected as priority compounds, one highly cytotoxic towards both MCF12A and MCF7 cell lines (RUMB-015) and one which was non toxic to either cell line (RUMB-017/018). Preliminary studies into the mechanism of cytotoxicity using Western blot analysis for poly (ADP-ribose) polymerase (PARP) cleavage and Hoechst 33342 immunostaining in MCF-7 cells were largely unsuccessful. The Hoechst 33342 immunostaining assay did provide tentative evidence that selected priority compounds were capable of inducing apoptosis, although these assays will need to be repeated using a less subjective assay to confirm the results. The priority compounds were subsequently investigated for their cytotoxic effect on the cancer stem cell-enriched side population in MCF7 cells. The ability of the priority compounds to selectively target the cancer stem cell containing side population was assessed using two complementary flow cytometry-based techniques – namely the Hoechst 33342-exclusion assay, and fluorescent immunostaining for the expression of the putative cancer stem cell marker, ABCG2+. The ABCG2+ staining assay was a novel technique developed during the course of this study. It remains to be fully validated, but it may provide a new and reliable way to identify and analyse cancer stem cell containing side population cells. The MCF7 cells were treated with the compounds and the proportion of putative cancer stem cells compared with the size of the population in untreated cells was assessed. Three compounds (RUMB-010, RUMB-015 and RUMB-017/018) capable of reducing the proportion of side population cells within the MCF7 cell line were identified. Taking these data together, we identified two potential „hit‟ compounds which should be prioritised for future research. These are compounds RUMB-010/sargaquinoic acid and RUMB-017/018. RUMB-010 is of interest as it was shown to target the putative cancer stem cell population, in addition to the bulk MCF7 tumour line, but was relatively less toxic to the „normal‟ MCF12A cell line. RUMB-017/018 is of interest due to the ability to selectively target the cancer stem cell enriched side population, while having little effect on the normal (MCF12A) or bulk tumour (MCF7) cell lines tested. These compounds will be important as „hit‟ compounds for drug development and as tool compounds to study cancer and cancer stem cell biology.
- Full Text:
- Date Issued: 2010
- Authors: Lawson, Jessica Clair
- Date: 2010
- Subjects: Breast -- Cancer , Breast -- Cancer -- Chemotherapy , Breast -- Cancer -- Treatment , Red algae , Brown algae , Algae -- Biotechnology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3925 , http://hdl.handle.net/10962/d1003984 , Breast -- Cancer , Breast -- Cancer -- Chemotherapy , Breast -- Cancer -- Treatment , Red algae , Brown algae , Algae -- Biotechnology
- Description: Breast cancer, the most common malignancy diagnosed in women, is one of the leading causes of death in women worldwide. In South Africa only 32% of women diagnosed with advanced breast cancer survive more than five years. The search for new chemotherapeutic agents capable of effectively treating breast cancer is therefore essential. Recent evidence supporting the cancer stem cell theory of cancer development for breast cancer challenges the current theories of cancer development and hence treatment. Cancer stem cells are a small subpopulation of tumour cells that possess properties of both cancer cells and stem cells and are believed to be the tumour-initiating population of many cancers. Cancer stem cells are inherently resistant to many chemotherapeutic agents and in this way have been associated with repopulation of tumours after chemotherapy. This phenomenon is proposed as a possible mechanism for cancer relapse after treatment. Cancer stem cells have also been implicated in metastasis, the major cause of mortality in cancer patients. Therefore, any treatment that is capable of targeting and removing breast cancer stem cells may have the theoretical potential to effectively treat breast cancer. However, there are currently no such treatments available for clinical use. We were provided access to a library of novel indigenous small molecules isolated from red and brown algae found off the Eastern Cape of South Africa. The aim of this project was to analyse the anti-cancer and anti-cancer stem cell properties of the compounds in this library and to identify „hit‟ compounds which could form the basis for future development into new anti-cancer drugs. Ten novel compounds of algal origin were tested for cytotoxicity, by determining their ability to inhibit the growth of MCF12A breast epithelial cells and MCF7 breast cancer cells using the colorimetric MTT [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] cell proliferation assay. All but one of the compounds tested exhibited cytotoxicity towards the MCF7 cancer cell line, with IC50 values (the concentration of the compound that leads to a 50% inhibition in cell growth) of between 3 μM and 90 μM. The chemotherapeutic drug paclitaxel was used as a positive control. Four of the compounds (RUMB-001, RUMB-002, RUMB-007 and RUMB-010/saragaquinoic acid) were significantly more toxic to the MCF7 cancer cell line, than the „normal‟ MCF12A breast cells and were selected as priority compounds for further analyses. In addition, two other compounds were selected as priority compounds, one highly cytotoxic towards both MCF12A and MCF7 cell lines (RUMB-015) and one which was non toxic to either cell line (RUMB-017/018). Preliminary studies into the mechanism of cytotoxicity using Western blot analysis for poly (ADP-ribose) polymerase (PARP) cleavage and Hoechst 33342 immunostaining in MCF-7 cells were largely unsuccessful. The Hoechst 33342 immunostaining assay did provide tentative evidence that selected priority compounds were capable of inducing apoptosis, although these assays will need to be repeated using a less subjective assay to confirm the results. The priority compounds were subsequently investigated for their cytotoxic effect on the cancer stem cell-enriched side population in MCF7 cells. The ability of the priority compounds to selectively target the cancer stem cell containing side population was assessed using two complementary flow cytometry-based techniques – namely the Hoechst 33342-exclusion assay, and fluorescent immunostaining for the expression of the putative cancer stem cell marker, ABCG2+. The ABCG2+ staining assay was a novel technique developed during the course of this study. It remains to be fully validated, but it may provide a new and reliable way to identify and analyse cancer stem cell containing side population cells. The MCF7 cells were treated with the compounds and the proportion of putative cancer stem cells compared with the size of the population in untreated cells was assessed. Three compounds (RUMB-010, RUMB-015 and RUMB-017/018) capable of reducing the proportion of side population cells within the MCF7 cell line were identified. Taking these data together, we identified two potential „hit‟ compounds which should be prioritised for future research. These are compounds RUMB-010/sargaquinoic acid and RUMB-017/018. RUMB-010 is of interest as it was shown to target the putative cancer stem cell population, in addition to the bulk MCF7 tumour line, but was relatively less toxic to the „normal‟ MCF12A cell line. RUMB-017/018 is of interest due to the ability to selectively target the cancer stem cell enriched side population, while having little effect on the normal (MCF12A) or bulk tumour (MCF7) cell lines tested. These compounds will be important as „hit‟ compounds for drug development and as tool compounds to study cancer and cancer stem cell biology.
- Full Text:
- Date Issued: 2010
An investigation into the journalistic identities of news workers at the state owned Lentsoe La Basotho/Lesotho Today Newspaper
- Authors: Kotele, Mothepane
- Date: 2010
- Subjects: Lentsoe La Basotho/Lesotho Today Mass media -- Economic aspects -- Lesotho Mass media -- Social aspects -- Lesotho Mass media -- Employees Journalists -- Lesotho Sotho newspapers Journalistic ethics -- Lesotho
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3449 , http://hdl.handle.net/10962/d1002903
- Description: Informed by the political economy framework and the public service role of media in democracy, the main objective of the study was to use in-depth semi-structured interviews to understand news-workers’ professional journalistic identities in relation to their status as government employees and the understanding of their public service role as outlined in the paper’s mission statement. The main interest was to understand the complexity of negotiating these role identities. Through reference to the theories of journalism professionalism, the study highlighted the extent to which news-workers in the small newsroom of Lentsoe la Basotho/Lesotho Today see themselves as public service journalists in a democratic country. The interest was borne partly out of the views of the paper’s critics who see it as not serving the public but rather promoting the activities and policies of the government of the day, thus falling short of its democratic role. The contention of the study was that as a public service newspaper, the paper should have news-workers who do impartial journalism and reflect the public’s right to know in their reporting. The findings of the study suggests that news-workers at Lentsoe la Basotho/Lesotho Today continuously have to strive to negotiate the potential conflict between being a professional and working for a government-controlled newspaper. While they sometimes lay claim to being journalists, the reality is that in their political coverage they end up adopting the role of government mouthpieces.
- Full Text:
- Date Issued: 2010
- Authors: Kotele, Mothepane
- Date: 2010
- Subjects: Lentsoe La Basotho/Lesotho Today Mass media -- Economic aspects -- Lesotho Mass media -- Social aspects -- Lesotho Mass media -- Employees Journalists -- Lesotho Sotho newspapers Journalistic ethics -- Lesotho
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3449 , http://hdl.handle.net/10962/d1002903
- Description: Informed by the political economy framework and the public service role of media in democracy, the main objective of the study was to use in-depth semi-structured interviews to understand news-workers’ professional journalistic identities in relation to their status as government employees and the understanding of their public service role as outlined in the paper’s mission statement. The main interest was to understand the complexity of negotiating these role identities. Through reference to the theories of journalism professionalism, the study highlighted the extent to which news-workers in the small newsroom of Lentsoe la Basotho/Lesotho Today see themselves as public service journalists in a democratic country. The interest was borne partly out of the views of the paper’s critics who see it as not serving the public but rather promoting the activities and policies of the government of the day, thus falling short of its democratic role. The contention of the study was that as a public service newspaper, the paper should have news-workers who do impartial journalism and reflect the public’s right to know in their reporting. The findings of the study suggests that news-workers at Lentsoe la Basotho/Lesotho Today continuously have to strive to negotiate the potential conflict between being a professional and working for a government-controlled newspaper. While they sometimes lay claim to being journalists, the reality is that in their political coverage they end up adopting the role of government mouthpieces.
- Full Text:
- Date Issued: 2010
Indigenous technology and culture in the technology curriculum : starting the conversation : a case study
- Authors: Vandeleur, Sonja
- Date: 2010
- Subjects: Curriculum evaluation -- South Africa Curriculum planning -- South Africa Education -- Curricula -- South Africa Technology -- Study and teaching -- South Africa Indigenous peoples -- Education -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1573 , http://hdl.handle.net/10962/d1003455
- Description: Since the collapse of apartheid and the first democratic elections of 1994, education in South Africa has undergone fundamental transformation and part of this transformation was the reconstruction of the school curriculum. The new curriculum, known as Curriculum 2005 and developed in 1997, introduced Technology as a new learning area. This study is based on the inclusion of ‘indigenous technology and culture’, a new aspect introduced in a revision of Curriculum 2005. The broad goal of the study was to examine and explore pedagogic practice in relation to the inclusion of ‘indigenous technology and culture’ in the revised National Curriculum Statement for Technology. The study was informed by an examination of literature pertaining to philosophy of technology, indigenous knowledge systems and technology education. The review of the literature highlighted the contested nature of ‘indigenous knowledge systems’. Philosophies on the nature of technological knowledge were reviewed in order to explore the meaning of ‘technology’, and a comparative review of curriculum reform in regard to technology education in various parts of the world was conducted. This study presented an attempt to determine the rationale for the inclusion of ‘indigenous technology and culture’ in the revised National Curriculum Statement for Technology in South Africa and to explore and examine what teachers’ existing practices were in this regard. It also examined a process of participatory co-engagement with a focus group of teachers. This process was an attempt to implement ‘indigenous technology and culture’ of the curriculum in a more meaningful way. A case study approach using an in-depth, interpretive design was used. A questionnaire, document analysis, interviews and focus group discussions were used to conduct the investigation. What emerged from the data analysis was that there was unanimous support for the inclusion of ‘indigenous technology and culture’ in the technology curriculum, but implementation had been problematic. This was partly due to difficulties with the interpretation of this aspect in the curriculum as well as a lack of meaningful teaching and learning for various reasons. The study revealed that teachers face multiple dilemmas in implementing ‘indigenous technology and culture’ as an assessment standard. These dilemmas are pedagogical, political, conceptual, professional and cultural in nature. The intentions of the study were to build a comprehensive understanding of ‘indigenous technology and culture’ and to determine how a focus group of teachers were dealing with this new inclusion. The interpretive study concluded with implications and recommendations for further studies.
- Full Text:
- Date Issued: 2010
- Authors: Vandeleur, Sonja
- Date: 2010
- Subjects: Curriculum evaluation -- South Africa Curriculum planning -- South Africa Education -- Curricula -- South Africa Technology -- Study and teaching -- South Africa Indigenous peoples -- Education -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1573 , http://hdl.handle.net/10962/d1003455
- Description: Since the collapse of apartheid and the first democratic elections of 1994, education in South Africa has undergone fundamental transformation and part of this transformation was the reconstruction of the school curriculum. The new curriculum, known as Curriculum 2005 and developed in 1997, introduced Technology as a new learning area. This study is based on the inclusion of ‘indigenous technology and culture’, a new aspect introduced in a revision of Curriculum 2005. The broad goal of the study was to examine and explore pedagogic practice in relation to the inclusion of ‘indigenous technology and culture’ in the revised National Curriculum Statement for Technology. The study was informed by an examination of literature pertaining to philosophy of technology, indigenous knowledge systems and technology education. The review of the literature highlighted the contested nature of ‘indigenous knowledge systems’. Philosophies on the nature of technological knowledge were reviewed in order to explore the meaning of ‘technology’, and a comparative review of curriculum reform in regard to technology education in various parts of the world was conducted. This study presented an attempt to determine the rationale for the inclusion of ‘indigenous technology and culture’ in the revised National Curriculum Statement for Technology in South Africa and to explore and examine what teachers’ existing practices were in this regard. It also examined a process of participatory co-engagement with a focus group of teachers. This process was an attempt to implement ‘indigenous technology and culture’ of the curriculum in a more meaningful way. A case study approach using an in-depth, interpretive design was used. A questionnaire, document analysis, interviews and focus group discussions were used to conduct the investigation. What emerged from the data analysis was that there was unanimous support for the inclusion of ‘indigenous technology and culture’ in the technology curriculum, but implementation had been problematic. This was partly due to difficulties with the interpretation of this aspect in the curriculum as well as a lack of meaningful teaching and learning for various reasons. The study revealed that teachers face multiple dilemmas in implementing ‘indigenous technology and culture’ as an assessment standard. These dilemmas are pedagogical, political, conceptual, professional and cultural in nature. The intentions of the study were to build a comprehensive understanding of ‘indigenous technology and culture’ and to determine how a focus group of teachers were dealing with this new inclusion. The interpretive study concluded with implications and recommendations for further studies.
- Full Text:
- Date Issued: 2010
Goat production in the Northern Cape: what are the impacts of farmer training?
- Authors: Burgess, Roberta
- Date: 2010
- Subjects: Northern Cape (South Africa) Animal culture -- South Africa -- Northern Cape -- Management Animal culture -- Economic aspects -- South Africa -- Northern Cape Goat farmers -- South Africa -- Northern Cape -- Education
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:747 , http://hdl.handle.net/10962/d1003868
- Description: The main millennium development goal is to eradicate extreme poverty and hunger in the world by 2015. South Africa is one of the countries that signed this pledge at the millennium summit in 2000. The Department of Agriculture, has initiated a number of projects to assist farmers and communities with reaching this goal. In the Northern Cape, the commercialization of goats is one such anchor project. The major activities of this project is to train farmers in applied and appropriate production practices, assist them with accessing markets and supporting them with improved livestock. The main aim of this research was to evaluate whether the project has achieved its outcome and whether this outcome, is having an impact on the livelihoods of the farmers involved in the project. After reviewing the literature on evaluation research and programme evaluation across time, the different paradigms and approaches, participatory methodology was used in this study. A case study approach was applied to evaluate the outcomes and effects of the training received by farmers, as part of the commercialization of goats programme. The results of the study proved that the training received by members of the Campbell Agricultural Co-operative Enterprises, changed their goat production practices. The training also had a secondary impact. Trained farmers were imparting (technology transfer) their acquired knowledge to other members of the co-operative. This appeared very effective as all members of the cooperative were farming more scientifically. These changes to the production practices had a positive impact on the livelihoods of the farmers, as their animals were of a better quality and they were able to sell their animals at different markets and for better prices. This has however not commercialized their farming operations as they are still faced with many challenges. These challenges are the lack of a proper marketing structure, access to financial assistance, proper infrastructure and adequate transport for the animals to mention a few. If the Department of Agriculture and Land Affairs is serious about assisting these farmers to move from the status of “emerging farmer” to “commercial farmer” they will have to look at addressing the problems experienced with financial access and access to adequate and appropriate transport.
- Full Text:
- Date Issued: 2010
- Authors: Burgess, Roberta
- Date: 2010
- Subjects: Northern Cape (South Africa) Animal culture -- South Africa -- Northern Cape -- Management Animal culture -- Economic aspects -- South Africa -- Northern Cape Goat farmers -- South Africa -- Northern Cape -- Education
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:747 , http://hdl.handle.net/10962/d1003868
- Description: The main millennium development goal is to eradicate extreme poverty and hunger in the world by 2015. South Africa is one of the countries that signed this pledge at the millennium summit in 2000. The Department of Agriculture, has initiated a number of projects to assist farmers and communities with reaching this goal. In the Northern Cape, the commercialization of goats is one such anchor project. The major activities of this project is to train farmers in applied and appropriate production practices, assist them with accessing markets and supporting them with improved livestock. The main aim of this research was to evaluate whether the project has achieved its outcome and whether this outcome, is having an impact on the livelihoods of the farmers involved in the project. After reviewing the literature on evaluation research and programme evaluation across time, the different paradigms and approaches, participatory methodology was used in this study. A case study approach was applied to evaluate the outcomes and effects of the training received by farmers, as part of the commercialization of goats programme. The results of the study proved that the training received by members of the Campbell Agricultural Co-operative Enterprises, changed their goat production practices. The training also had a secondary impact. Trained farmers were imparting (technology transfer) their acquired knowledge to other members of the co-operative. This appeared very effective as all members of the cooperative were farming more scientifically. These changes to the production practices had a positive impact on the livelihoods of the farmers, as their animals were of a better quality and they were able to sell their animals at different markets and for better prices. This has however not commercialized their farming operations as they are still faced with many challenges. These challenges are the lack of a proper marketing structure, access to financial assistance, proper infrastructure and adequate transport for the animals to mention a few. If the Department of Agriculture and Land Affairs is serious about assisting these farmers to move from the status of “emerging farmer” to “commercial farmer” they will have to look at addressing the problems experienced with financial access and access to adequate and appropriate transport.
- Full Text:
- Date Issued: 2010
The relationship between leadership style and employee engagement in Sasol Gas, South Africa
- Authors: Duma, Thobile
- Date: 2010
- Language: English
- Type: text , Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10962/64034 , vital:28524
- Description: The main purpose of this study was to determine whether there is a significant relationship between leadership styles of Sasol Gas leaders and their followers’ level of employee engagement. The instruments used in the study were the Multifactor Leadership Questionnaire (MLQ) (Form 5X) and the Utrecht Work Engagement Scale (UWES 9). The MLQ (5X) was completed by the followers and leaders of Sasol Gas to determine whether Sasol Gas leaders use transactional, transformational and/or laissez-faire leadership styles. The work engagement instrument was completed by each follower to determine current levels of employee engagement. Descriptive statistics were obtained and correlations completed for the data to determine whether the different leadership styles exhibit different levels of engagement. The MLQ survey results indicate that leaders of Sasol Gas have more transformational than transactional leadership styles. The UWES 9 measured the three factors of vigour, dedication, and absorption; dedication had the highest mean compared to vigour and absorption, indicating that Sasol Gas employees strongly identify themselves with their work because it is experienced as meaningful, inspiring, and challenging. This research found a statistically significant, albeit weak, relationship between transformational and transactional leadership styles and employee engagement. This is a positive reflection of the current Sasol Gas leaders because empirical evidence shows that transformational leadership behaviours are more successful for attaining and fulfilling goals during organisational change.
- Full Text:
- Date Issued: 2010
- Authors: Duma, Thobile
- Date: 2010
- Language: English
- Type: text , Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10962/64034 , vital:28524
- Description: The main purpose of this study was to determine whether there is a significant relationship between leadership styles of Sasol Gas leaders and their followers’ level of employee engagement. The instruments used in the study were the Multifactor Leadership Questionnaire (MLQ) (Form 5X) and the Utrecht Work Engagement Scale (UWES 9). The MLQ (5X) was completed by the followers and leaders of Sasol Gas to determine whether Sasol Gas leaders use transactional, transformational and/or laissez-faire leadership styles. The work engagement instrument was completed by each follower to determine current levels of employee engagement. Descriptive statistics were obtained and correlations completed for the data to determine whether the different leadership styles exhibit different levels of engagement. The MLQ survey results indicate that leaders of Sasol Gas have more transformational than transactional leadership styles. The UWES 9 measured the three factors of vigour, dedication, and absorption; dedication had the highest mean compared to vigour and absorption, indicating that Sasol Gas employees strongly identify themselves with their work because it is experienced as meaningful, inspiring, and challenging. This research found a statistically significant, albeit weak, relationship between transformational and transactional leadership styles and employee engagement. This is a positive reflection of the current Sasol Gas leaders because empirical evidence shows that transformational leadership behaviours are more successful for attaining and fulfilling goals during organisational change.
- Full Text:
- Date Issued: 2010
The value of a Rhodes University degree and securing employment
- Authors: Chambers, Tracy Laura
- Date: 2010
- Subjects: Rhodes University -- Graduate students -- Employment Education, Higher -- South Africa Graduate students -- Employment -- South Africa Labor supply -- Effect of education on -- South Africa Economic development -- Effect of education on -- South Africa Labor market -- South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:769 , http://hdl.handle.net/10962/d1003890
- Description: In South Africa, reform policies and frameworks introduced since the 1994 democratic election have sought to bring about change to a highly ineffective and unfair system. In spite of all the changes which have occurred, however, there is evidence to suggest that the system is still not functioning as effectively as it might, given that a relatively large number of graduates remain unemployed in a country with a high skills shortage. This thesis aims to explore the experiences of graduates from one university, Rhodes University in the Eastern Cape, as they enter the job market. It does this through the administration of a first job destination survey administered at the 2009 Graduation Ceremonies held in Grahamstown. Analysis of the survey takes into account the idea that it is not a degree per se, or even a degree from a prestigious university which brings employment, but also the social, cultural and human capital that graduates can bring to their job search.
- Full Text:
- Date Issued: 2010
- Authors: Chambers, Tracy Laura
- Date: 2010
- Subjects: Rhodes University -- Graduate students -- Employment Education, Higher -- South Africa Graduate students -- Employment -- South Africa Labor supply -- Effect of education on -- South Africa Economic development -- Effect of education on -- South Africa Labor market -- South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:769 , http://hdl.handle.net/10962/d1003890
- Description: In South Africa, reform policies and frameworks introduced since the 1994 democratic election have sought to bring about change to a highly ineffective and unfair system. In spite of all the changes which have occurred, however, there is evidence to suggest that the system is still not functioning as effectively as it might, given that a relatively large number of graduates remain unemployed in a country with a high skills shortage. This thesis aims to explore the experiences of graduates from one university, Rhodes University in the Eastern Cape, as they enter the job market. It does this through the administration of a first job destination survey administered at the 2009 Graduation Ceremonies held in Grahamstown. Analysis of the survey takes into account the idea that it is not a degree per se, or even a degree from a prestigious university which brings employment, but also the social, cultural and human capital that graduates can bring to their job search.
- Full Text:
- Date Issued: 2010