Developing an attractant for monitoring fruit-feeding moths in citrus orchards
- Authors: Goddard, Mathew Keith
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2981 , vital:20349
- Description: Fruit-piercing moths are a sporadic pest of citrus, especially in the Eastern Cape Province of South Africa, where the adults can cause significant damage in outbreak years. Currently the only way in which to successfully control fruit-feeding moths within the orchards is the use of repellent lights. However, growers confuse fruit-piercing moths with fruit-sucking moths that don‘t cause primary damage, and there is no way of monitoring which moth species are attacking the fruit in the orchards during the night. In a previous study, banana was shown to be the most attractive bait for a variety of fruit-feeding moth species. Therefore the aim of this study was to determine the population dynamics of fruit-feeding moths develop a cost-effective alternative to the use of fresh banana as a bait for fruit-piercing moths. Fresh banana was compared to nine alternative synthetic attractants, frozen banana and a control under field conditions in several orchards in the Eastern Cape Province. Once again, banana was shown to be the most attractive bait. Some 23 species of fruit-feeding moth species were sampled in the traps, but there was only two fruit-piercing species, Serrodes partita (Fabricius) (Lepidoptera: Noctuidae) and Eudocima sp. Surprisingly S. partita, which was thought to be the main pest, comprised only 6.9% of trap catches. Serrodes partita, is a sporadic pest, only becoming problematic every five to 10 years after good rainfall in the Little Karoo region that causes flushes of their larval host, wild plum, Pappea capensis (Ecklon and Zeyher). During these outbreaks, damage to fruit can range from 70 to 90% and this is especially so for soft skinned citrus. A study on the morphology of the proboscis confirmed that only two species of fruit-piercing moths were present. Trap catches over three citrus growing seasons was linked to fruit damage found within several orchards. Once again fruit-piercing moth damage was relatively low in comparison to other types of damage such as mechanical and undefined damage. There was a very weak correlation between S. partita trap catches and damage, but generally damage was recorded two to three weeks after a peak in S. partita trap catches. Climatic conditions were also recorded and compared to weekly trap catches of S. partita, and while temperature and wind direction had no influence on moth populations, precipitation in the orchards was weakly correlated with trap catches. This study has shown that in non-outbreak seasons, the main fruit-piercing moth, S. partita comprises a small percentage of fruit-feeding moths in citrus orchards, but that growers are unable to determine the difference between fruit-piercing species and the harmless fruit-sucking species. Further fresh banana remains the best method for attracting fruit-piecing moths to traps, but this is not cost effective and thus a commercially viable protocol for monitoring these species remains elusive.
- Full Text:
- Date Issued: 2016
- Authors: Goddard, Mathew Keith
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2981 , vital:20349
- Description: Fruit-piercing moths are a sporadic pest of citrus, especially in the Eastern Cape Province of South Africa, where the adults can cause significant damage in outbreak years. Currently the only way in which to successfully control fruit-feeding moths within the orchards is the use of repellent lights. However, growers confuse fruit-piercing moths with fruit-sucking moths that don‘t cause primary damage, and there is no way of monitoring which moth species are attacking the fruit in the orchards during the night. In a previous study, banana was shown to be the most attractive bait for a variety of fruit-feeding moth species. Therefore the aim of this study was to determine the population dynamics of fruit-feeding moths develop a cost-effective alternative to the use of fresh banana as a bait for fruit-piercing moths. Fresh banana was compared to nine alternative synthetic attractants, frozen banana and a control under field conditions in several orchards in the Eastern Cape Province. Once again, banana was shown to be the most attractive bait. Some 23 species of fruit-feeding moth species were sampled in the traps, but there was only two fruit-piercing species, Serrodes partita (Fabricius) (Lepidoptera: Noctuidae) and Eudocima sp. Surprisingly S. partita, which was thought to be the main pest, comprised only 6.9% of trap catches. Serrodes partita, is a sporadic pest, only becoming problematic every five to 10 years after good rainfall in the Little Karoo region that causes flushes of their larval host, wild plum, Pappea capensis (Ecklon and Zeyher). During these outbreaks, damage to fruit can range from 70 to 90% and this is especially so for soft skinned citrus. A study on the morphology of the proboscis confirmed that only two species of fruit-piercing moths were present. Trap catches over three citrus growing seasons was linked to fruit damage found within several orchards. Once again fruit-piercing moth damage was relatively low in comparison to other types of damage such as mechanical and undefined damage. There was a very weak correlation between S. partita trap catches and damage, but generally damage was recorded two to three weeks after a peak in S. partita trap catches. Climatic conditions were also recorded and compared to weekly trap catches of S. partita, and while temperature and wind direction had no influence on moth populations, precipitation in the orchards was weakly correlated with trap catches. This study has shown that in non-outbreak seasons, the main fruit-piercing moth, S. partita comprises a small percentage of fruit-feeding moths in citrus orchards, but that growers are unable to determine the difference between fruit-piercing species and the harmless fruit-sucking species. Further fresh banana remains the best method for attracting fruit-piecing moths to traps, but this is not cost effective and thus a commercially viable protocol for monitoring these species remains elusive.
- Full Text:
- Date Issued: 2016
A psychobiographical study of Charlize Theron
- Authors: Prenter, Tracey
- Date: 2016
- Subjects: Theron, Charlize -- Psychology , Motion picture actors and actresses -- South Africa -- Biography , Motion picture actors and actresses -- United States -- Biography , Psychology -- Biographical methods
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3272 , http://hdl.handle.net/10962/d1020843
- Description: Psychobiographers study the lives of extraordinary, prominent, and enigmatic individuals. Psychobiographical research advances our insight into the uniqueness and complexity of the human personality and therefore makes a substantial contribution towards one of the major objectives of the field of psychology. Purposive sampling was employed to select Charlize Theron as the subject of this psychobiographical study. As the only South African who has won an Oscar, Theron is an exceptional individual who demonstrates tenacity and a will to succeed despite significant traumatic events in her childhood. The case study data was organised and analysed according to the general analytic approach developed by Huberman and Miles (2002) and one of Alexander’s (1990) strategies, namely questioning the data. Erikson’s psychosocial theory (1950, 1963, 1995) was selected to guide this study because it recognises the impact of socio-cultural influences on developmental processes and provides a comprehensive, staged framework for studying Theron’s personality development. This study contributes to the development of psychobiographical research in South Africa.
- Full Text:
- Date Issued: 2016
- Authors: Prenter, Tracey
- Date: 2016
- Subjects: Theron, Charlize -- Psychology , Motion picture actors and actresses -- South Africa -- Biography , Motion picture actors and actresses -- United States -- Biography , Psychology -- Biographical methods
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3272 , http://hdl.handle.net/10962/d1020843
- Description: Psychobiographers study the lives of extraordinary, prominent, and enigmatic individuals. Psychobiographical research advances our insight into the uniqueness and complexity of the human personality and therefore makes a substantial contribution towards one of the major objectives of the field of psychology. Purposive sampling was employed to select Charlize Theron as the subject of this psychobiographical study. As the only South African who has won an Oscar, Theron is an exceptional individual who demonstrates tenacity and a will to succeed despite significant traumatic events in her childhood. The case study data was organised and analysed according to the general analytic approach developed by Huberman and Miles (2002) and one of Alexander’s (1990) strategies, namely questioning the data. Erikson’s psychosocial theory (1950, 1963, 1995) was selected to guide this study because it recognises the impact of socio-cultural influences on developmental processes and provides a comprehensive, staged framework for studying Theron’s personality development. This study contributes to the development of psychobiographical research in South Africa.
- Full Text:
- Date Issued: 2016
Eye of a needle
- Authors: Fick, Cornelia
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6003 , http://hdl.handle.net/10962/d1021210
- Description: Most of my stories are about interpersonal relationships between the sexes, as well as intrapersonal processes, such as growing old. I have a deep connection to such themes because of my background as a general nurse and midwife; meeting too many abused women in hospitals, and the broader community. Because patterns of abuse tend to become invisible, I use experimental forms of storytelling as well as sharp, ironic and dark humour as a way to make this side of life more visible. My reading has shown me how experimental forms can render seemingly timeless or ageless topics in a fresh, vital way.
- Full Text:
- Date Issued: 2016
- Authors: Fick, Cornelia
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6003 , http://hdl.handle.net/10962/d1021210
- Description: Most of my stories are about interpersonal relationships between the sexes, as well as intrapersonal processes, such as growing old. I have a deep connection to such themes because of my background as a general nurse and midwife; meeting too many abused women in hospitals, and the broader community. Because patterns of abuse tend to become invisible, I use experimental forms of storytelling as well as sharp, ironic and dark humour as a way to make this side of life more visible. My reading has shown me how experimental forms can render seemingly timeless or ageless topics in a fresh, vital way.
- Full Text:
- Date Issued: 2016
Notebook of unremembered poems
- Authors: McKeown, Jean Wallace
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6013 , http://hdl.handle.net/10962/d1021233
- Description: My poems contain narrative elements and explore themes of identity, motherhood, sexuality, and fear of relinquishing control. Sharon Olds, in her book Stag’s Leap, sums up my intention: “and I saw again how blessed my life has been, / first, to have been able to love, / then, to have the parting now behind me.” My collection chronicles a path towards acceptance of self from childhood onwards, and, more than that, a pleasure and pride in self, and I have tried to find the forms which will reflect this path in the reader’s own experience. Most of the poems are written in a conversational voice and a free-form style which gives me creative licence to explore transition and transformation.
- Full Text:
- Date Issued: 2016
- Authors: McKeown, Jean Wallace
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6013 , http://hdl.handle.net/10962/d1021233
- Description: My poems contain narrative elements and explore themes of identity, motherhood, sexuality, and fear of relinquishing control. Sharon Olds, in her book Stag’s Leap, sums up my intention: “and I saw again how blessed my life has been, / first, to have been able to love, / then, to have the parting now behind me.” My collection chronicles a path towards acceptance of self from childhood onwards, and, more than that, a pleasure and pride in self, and I have tried to find the forms which will reflect this path in the reader’s own experience. Most of the poems are written in a conversational voice and a free-form style which gives me creative licence to explore transition and transformation.
- Full Text:
- Date Issued: 2016
An erosion and sediment delivery model for semi-arid catchments
- Authors: Bryson, Louise Kay
- Date: 2016
- Subjects: Sedimentation and deposition , Erosion , Watershed management -- South Africa , Water-supply -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6056 , http://hdl.handle.net/10962/d1020331
- Description: Sedimentation has become a significant environmental threat in South Africa as it intensifies water management problems in the water-scarce semi-arid regions of the country. As South Africa already allocates 98% of available water, the loss of storage capacity in reservoirs and degraded water quality has meant that a reliable water supply is compromised. The overall aim of this thesis was to develop a catchment scale model that represents the sediment dynamics of semi-arid regions of South Africa as a simple and practically applicable tool for water resource managers. Development of a conceptual framework for the model relied on an understanding of both the sediment dynamics of South African catchments and applicable modelling techniques. Scale was an issue in both cases as most of our understanding of the physical processes of runoff generation and sediment transport has been derived from plot scale studies. By identifying defining properties of semi-arid catchments it was possible to consider how temporal and spatial properties at higher levels emerged from properties at lower levels. These properties were effectively represented by using the Pitman rainfall-runoff model disaggregated to a daily timescale, the Modified Universal Soil Loss Equation (MUSLE) model incorporating probability function theory and through the representation of sediment storages across a semi-distributed catchment. The model was tested on two small and one large study catchment in the Karoo, South Africa, with limited observed data. Limitations to the model were found to be the large parameter data set and the dominance of structural constraints with an increase in catchment size. The next steps in model development will require a reduction of the parameter data set and an inclusion of an in-stream component for sub-catchments at a larger spatial scale. The model is applicable in areas such as South Africa where water resource managers need a simple model at the catchment scale in order to make decisions. This type of model provides a simple representation of the stochastic nature of erosion and sediment delivery over large spatial and temporal scales.
- Full Text:
- Date Issued: 2016
- Authors: Bryson, Louise Kay
- Date: 2016
- Subjects: Sedimentation and deposition , Erosion , Watershed management -- South Africa , Water-supply -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6056 , http://hdl.handle.net/10962/d1020331
- Description: Sedimentation has become a significant environmental threat in South Africa as it intensifies water management problems in the water-scarce semi-arid regions of the country. As South Africa already allocates 98% of available water, the loss of storage capacity in reservoirs and degraded water quality has meant that a reliable water supply is compromised. The overall aim of this thesis was to develop a catchment scale model that represents the sediment dynamics of semi-arid regions of South Africa as a simple and practically applicable tool for water resource managers. Development of a conceptual framework for the model relied on an understanding of both the sediment dynamics of South African catchments and applicable modelling techniques. Scale was an issue in both cases as most of our understanding of the physical processes of runoff generation and sediment transport has been derived from plot scale studies. By identifying defining properties of semi-arid catchments it was possible to consider how temporal and spatial properties at higher levels emerged from properties at lower levels. These properties were effectively represented by using the Pitman rainfall-runoff model disaggregated to a daily timescale, the Modified Universal Soil Loss Equation (MUSLE) model incorporating probability function theory and through the representation of sediment storages across a semi-distributed catchment. The model was tested on two small and one large study catchment in the Karoo, South Africa, with limited observed data. Limitations to the model were found to be the large parameter data set and the dominance of structural constraints with an increase in catchment size. The next steps in model development will require a reduction of the parameter data set and an inclusion of an in-stream component for sub-catchments at a larger spatial scale. The model is applicable in areas such as South Africa where water resource managers need a simple model at the catchment scale in order to make decisions. This type of model provides a simple representation of the stochastic nature of erosion and sediment delivery over large spatial and temporal scales.
- Full Text:
- Date Issued: 2016
Genetic and biological characterisation of a novel South African Cydia pomonella granulovirus (CpGV-SA) isolate
- Motsoeneng, Boitumelo Madika
- Authors: Motsoeneng, Boitumelo Madika
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:20503 , http://hdl.handle.net/10962/d1021266
- Description: The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is the primary pest of pome fruit cultivated worldwide. The control of this insect pest has been dependent on the frequent use of broad-spectrum chemical pesticides, which has led to the development of resistance in pest populations and negative effects on human health and the environment. The Betabaculovirus of C. pomonella has successfully been applied as a biological control agent in integrated pest management (IPM) programmes for the suppression of pest populations worldwide. Previously, all Cydia pomonella granulovirus (CpGV) biopesticides were based on a Mexican isolate (CpGV-M) and although these products are highly efficient at controlling C. pomonella, resistance cases have been reported across Europe. The identification of novel CpGV isolates as additional or alternative control agents to manage resistance is therefore necessary. This study aimed to genetically and biologically characterise a novel South African C. pomonella granulovirus isolate and to test its virulence against neonate larvae. Based on the morphology of the occlusion bodies observed using transmission electron microscopy, granuloviruses were recovered from diseased and dead larvae collected from an orchard in South Africa where no virus applications had been made. DNA was extracted and the identification of the isolated granulovirus was achieved through the PCR amplification and sequencing of the lef-8, lef-9, granulin and egt genes. Submission of the gene sequences to BLAST revealed high percentage identities to sequences from various CpGV isolates, resulting in the naming of the isolate in this study as the South African Cydia pomonella granulovirus (CpGV-SA) isolate. Phylogenetic analysis based on the single nucleotide polymorphisms (SNPs) detected in the lef-8, lef-9 and granulin nucleotide sequences grouped the South African isolate with CpGV-E2 (genome type B) and CpGV-S (genome type E). The CpGV-SA isolate was further genetically characterised by restriction endonuclease analysis and complete sequencing of the genomic DNA. Differences were observed for the BamHI, EcoRI, PstI and XhoI profiles of CpGV-SA in comparison to the respective profiles generated for CpGV-M extracted from a biopesticide, Carpovirusine® (Arysta Lifescience, France). Several genetic variations between the complete genome sequence of CpGV-SA and the reference isolate, CpGV-M1, as well as a recent genome submission of CpGV-M, both representing genome type A were observed. The complete genome analysis confirmed that CpGV-SA is genetically different from the Mexican CpGV isolate, used in thedevelopment of most biopesticides. In silico restriction profiles of the genome sequence obtained for CpGV-SA and genome sequences of genetically different CpGV isolates originating from Mexico (M1 and M), England (E2), Canada (S) and Iran (I12 and I07), available on the NCBI’s GenBank database confirmed that CpGV-SA is of mixed genotypes. Furthermore, the South African isolate shared the single common difference found in the pe38 gene of resistance overcoming isolates, which was the absence of an internal 24 nucleotide repeat present in CpGV-M1. In addition to the common difference, SNPs detected in the pe38 gene grouped the isolate with the CpGV-S isolate, suggesting that the CpGV-SA isolate is predominantly of genome type E. To determine the biological activity of CpGV-SA against neonate C. pomonella larvae, surface bioassays were conducted alongside CpGV-M (Carpovirusine®) bioassays. The LC50 and LC90 values for the South African isolate were 1.6 × 103 and 1.2 × 105 OBs/ml respectively. The LT50 was determined to be 135 hours. These values were similar to the values obtained for CpGV-M (Carpovirusine®). The results in this study suggest that a novel South African CpGV isolate of mixed genotypes, potentially able to overcome resistance in C. pomonella, with biological activity similar to CpGV-M (Carpovirusine®) and important for the control of C. pomonella was recovered. The CpGV-SA isolate could therefore potentially be developed into a biopesticide for use in resistance management strategies against C. pomonella populations in South Africa.
- Full Text:
- Date Issued: 2016
- Authors: Motsoeneng, Boitumelo Madika
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:20503 , http://hdl.handle.net/10962/d1021266
- Description: The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is the primary pest of pome fruit cultivated worldwide. The control of this insect pest has been dependent on the frequent use of broad-spectrum chemical pesticides, which has led to the development of resistance in pest populations and negative effects on human health and the environment. The Betabaculovirus of C. pomonella has successfully been applied as a biological control agent in integrated pest management (IPM) programmes for the suppression of pest populations worldwide. Previously, all Cydia pomonella granulovirus (CpGV) biopesticides were based on a Mexican isolate (CpGV-M) and although these products are highly efficient at controlling C. pomonella, resistance cases have been reported across Europe. The identification of novel CpGV isolates as additional or alternative control agents to manage resistance is therefore necessary. This study aimed to genetically and biologically characterise a novel South African C. pomonella granulovirus isolate and to test its virulence against neonate larvae. Based on the morphology of the occlusion bodies observed using transmission electron microscopy, granuloviruses were recovered from diseased and dead larvae collected from an orchard in South Africa where no virus applications had been made. DNA was extracted and the identification of the isolated granulovirus was achieved through the PCR amplification and sequencing of the lef-8, lef-9, granulin and egt genes. Submission of the gene sequences to BLAST revealed high percentage identities to sequences from various CpGV isolates, resulting in the naming of the isolate in this study as the South African Cydia pomonella granulovirus (CpGV-SA) isolate. Phylogenetic analysis based on the single nucleotide polymorphisms (SNPs) detected in the lef-8, lef-9 and granulin nucleotide sequences grouped the South African isolate with CpGV-E2 (genome type B) and CpGV-S (genome type E). The CpGV-SA isolate was further genetically characterised by restriction endonuclease analysis and complete sequencing of the genomic DNA. Differences were observed for the BamHI, EcoRI, PstI and XhoI profiles of CpGV-SA in comparison to the respective profiles generated for CpGV-M extracted from a biopesticide, Carpovirusine® (Arysta Lifescience, France). Several genetic variations between the complete genome sequence of CpGV-SA and the reference isolate, CpGV-M1, as well as a recent genome submission of CpGV-M, both representing genome type A were observed. The complete genome analysis confirmed that CpGV-SA is genetically different from the Mexican CpGV isolate, used in thedevelopment of most biopesticides. In silico restriction profiles of the genome sequence obtained for CpGV-SA and genome sequences of genetically different CpGV isolates originating from Mexico (M1 and M), England (E2), Canada (S) and Iran (I12 and I07), available on the NCBI’s GenBank database confirmed that CpGV-SA is of mixed genotypes. Furthermore, the South African isolate shared the single common difference found in the pe38 gene of resistance overcoming isolates, which was the absence of an internal 24 nucleotide repeat present in CpGV-M1. In addition to the common difference, SNPs detected in the pe38 gene grouped the isolate with the CpGV-S isolate, suggesting that the CpGV-SA isolate is predominantly of genome type E. To determine the biological activity of CpGV-SA against neonate C. pomonella larvae, surface bioassays were conducted alongside CpGV-M (Carpovirusine®) bioassays. The LC50 and LC90 values for the South African isolate were 1.6 × 103 and 1.2 × 105 OBs/ml respectively. The LT50 was determined to be 135 hours. These values were similar to the values obtained for CpGV-M (Carpovirusine®). The results in this study suggest that a novel South African CpGV isolate of mixed genotypes, potentially able to overcome resistance in C. pomonella, with biological activity similar to CpGV-M (Carpovirusine®) and important for the control of C. pomonella was recovered. The CpGV-SA isolate could therefore potentially be developed into a biopesticide for use in resistance management strategies against C. pomonella populations in South Africa.
- Full Text:
- Date Issued: 2016
Investigating a mathematics recovery program for assessment and intervention with groups of Grade 4 learners
- Authors: Wasserman, Anelia
- Date: 2016
- Subjects: Mathematics -- Study and teaching (Primary) , Mathematics -- Evaluation
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2065 , http://hdl.handle.net/10962/d1020340
- Description: This study reports on the findings of my research, which was based on an intervention focused on recovery of early arithmetic strategies with one Grade 4 class of learners in a township school in Port Elizabeth in the Eastern Cape. Learners came from poor socio-economic backgrounds and initial evaluations showed that the majority of learners still relied on concrete methods, like tally counting, to perform addition and subtraction calculations even with numbers less than 10. This is not uncommon in the South African context especially with learners in low Socio-economic Status (SES) schools. The results of numerous assessments including the Department of Education’s Annual National Assessments point to a crisis in primary mathematics education where intermediate phase learners are generally operating several grade levels below the grade they are in. A large drop in mathematics performance is seen in the ANA results in grade 4 learners (the first grade of the transition from foundation phase to intermediate phase). Within this context, and my background in learning support for students, my research aimed to understand the possibilities and constraints of the implementation of a recovery program adapted from the widely implemented work of Wright et al. (2006, 2012). The primary adaptation made to the MR program involved administering the assessments and intervention with groups of (rather than individual) learners. Within the context of the many low SES under-resourced schools in SA, individualised interview based assessments and recovery is not seen as a possible remediation strategy. Drawing on a socio- constructivist perspective, my study used action research with one class of 23 learners and found that adaptation of the MR program for a group, based on eight recovery sessions, was useful for enabling some progress for all learners in terms of their early arithmetic strategies and conceptual place value. Although the need for a longer recovery period is acknowledged, the adapted program enabled some progress in levels and stages of conceptual knowledge (as conceptualized by Wright et al.’s (2006) Learning Framework in Number) for these two domains. The study concludes with some reflections and recommendations for the future.
- Full Text:
- Date Issued: 2016
- Authors: Wasserman, Anelia
- Date: 2016
- Subjects: Mathematics -- Study and teaching (Primary) , Mathematics -- Evaluation
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2065 , http://hdl.handle.net/10962/d1020340
- Description: This study reports on the findings of my research, which was based on an intervention focused on recovery of early arithmetic strategies with one Grade 4 class of learners in a township school in Port Elizabeth in the Eastern Cape. Learners came from poor socio-economic backgrounds and initial evaluations showed that the majority of learners still relied on concrete methods, like tally counting, to perform addition and subtraction calculations even with numbers less than 10. This is not uncommon in the South African context especially with learners in low Socio-economic Status (SES) schools. The results of numerous assessments including the Department of Education’s Annual National Assessments point to a crisis in primary mathematics education where intermediate phase learners are generally operating several grade levels below the grade they are in. A large drop in mathematics performance is seen in the ANA results in grade 4 learners (the first grade of the transition from foundation phase to intermediate phase). Within this context, and my background in learning support for students, my research aimed to understand the possibilities and constraints of the implementation of a recovery program adapted from the widely implemented work of Wright et al. (2006, 2012). The primary adaptation made to the MR program involved administering the assessments and intervention with groups of (rather than individual) learners. Within the context of the many low SES under-resourced schools in SA, individualised interview based assessments and recovery is not seen as a possible remediation strategy. Drawing on a socio- constructivist perspective, my study used action research with one class of 23 learners and found that adaptation of the MR program for a group, based on eight recovery sessions, was useful for enabling some progress for all learners in terms of their early arithmetic strategies and conceptual place value. Although the need for a longer recovery period is acknowledged, the adapted program enabled some progress in levels and stages of conceptual knowledge (as conceptualized by Wright et al.’s (2006) Learning Framework in Number) for these two domains. The study concludes with some reflections and recommendations for the future.
- Full Text:
- Date Issued: 2016
Exploration, monetization, disillusion: a history of upstream oil development in the onshore Algoa basin
- Authors: James, Jonathan Scott
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3870 , vital:20551
- Description: The onshore Algoa basin has, since the mid-1960s, been an area of interest for oil and gas exploration. Despite the general lack of knowledge and publicly available information on the topic, a large amount of geological and geophysical data has been collected on the region owing to the oil and gas exploration. The intended aim of this thesis is to compile and construct a historical narrative of the oil and gas exploration that took place within the onshore Algoa basin, and to then contextualize that localized narrative within the greater macro-narrative of the global oil and gas industry. This thesis is primarily concerned with the time period beginning in the early 1960s up to mid-2014, however reference is also made to events pre-1960. For the purposes of compartmentalizing the various areas of research covered, the thesis has been divided into three broad areas of interest: the geology of the onshore Algoa basin, the global oil market and its impact on exploration therein, and the attempts to monetize the leases that came to be purchased post-exploration. The narrative on the geology of the onshore Algoa basin is aimed at providing a summarized account of the most important details pertaining to the search for petroleum systems in simplified, yet accurate, language. The aspects of the geology which command the most attention are those which are necessary in functioning petroleum systems such as suitable permeabilities, porosities, reservoir rocks, trapping mechanisms and cap rocks. The global oil and gas market is also used to contextualize the search for oil and gas within the onshore Algoa basin and is explained against the backdrop of the global oil trade and the sanctions imposed on the apartheid state. Furthermore, the analysis of the attempts to monetize leases within the onshore Algoa basin will provide a financial reference point to the shortcomings of the exploration and monetization efforts. The purpose of this thesis is to construct a historical narrative of the onshore Algoa basin which not only gives an accurate portrayal of the exploration efforts that have taken place thus far, but to also provide a enough detail of those exploration efforts to indicate the future of the onshore Algoa basin as an exploration play.
- Full Text:
- Date Issued: 2016
- Authors: James, Jonathan Scott
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3870 , vital:20551
- Description: The onshore Algoa basin has, since the mid-1960s, been an area of interest for oil and gas exploration. Despite the general lack of knowledge and publicly available information on the topic, a large amount of geological and geophysical data has been collected on the region owing to the oil and gas exploration. The intended aim of this thesis is to compile and construct a historical narrative of the oil and gas exploration that took place within the onshore Algoa basin, and to then contextualize that localized narrative within the greater macro-narrative of the global oil and gas industry. This thesis is primarily concerned with the time period beginning in the early 1960s up to mid-2014, however reference is also made to events pre-1960. For the purposes of compartmentalizing the various areas of research covered, the thesis has been divided into three broad areas of interest: the geology of the onshore Algoa basin, the global oil market and its impact on exploration therein, and the attempts to monetize the leases that came to be purchased post-exploration. The narrative on the geology of the onshore Algoa basin is aimed at providing a summarized account of the most important details pertaining to the search for petroleum systems in simplified, yet accurate, language. The aspects of the geology which command the most attention are those which are necessary in functioning petroleum systems such as suitable permeabilities, porosities, reservoir rocks, trapping mechanisms and cap rocks. The global oil and gas market is also used to contextualize the search for oil and gas within the onshore Algoa basin and is explained against the backdrop of the global oil trade and the sanctions imposed on the apartheid state. Furthermore, the analysis of the attempts to monetize leases within the onshore Algoa basin will provide a financial reference point to the shortcomings of the exploration and monetization efforts. The purpose of this thesis is to construct a historical narrative of the onshore Algoa basin which not only gives an accurate portrayal of the exploration efforts that have taken place thus far, but to also provide a enough detail of those exploration efforts to indicate the future of the onshore Algoa basin as an exploration play.
- Full Text:
- Date Issued: 2016
Dominion: architecture as a symbol of authority in the Eastern Cape Colonial Frontier
- Authors: Mnyila, Desmond
- Date: 2016
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: http://hdl.handle.net/10962/4256 , vital:20639
- Description: My mini thesis is an exploration of architecture as part of the landscape of Grahamstown and how ideas of dominion and subordination of the non- white citizens of this town were asserted or communicated through space. I concur with theories about architectural buildings as objects that express power and reinforce power relations in any given society. Markus (1993) goes into great lengths to explain how buildings are primarily about power and town planning is a means of control. The area under consideration is very rich in history especially during the period that interested me which is the nineteenth century as this was a period of the establishment of Grahamstown, firstly, as a military establishment and then as a small town serving as a residential area for the British Settlers who arrived in 1820. Throughout the mini thesis I have unpacked the nature of power itself by referring to Njoh (2009) who refers to different categories of the use and especially the abuse or demonstration of power. It wouldn’t do justice to an area as rich in history as the area which is now referred to as the Albany to not dwell into some of the events that were played out here, some of which had consequences and implications for the rest of South Africa. After 1820, the town developed as more buildings of domestic houses, churches, houses of officials, prisons and schools were built. In the thesis I unpack the different architectural styles like the Georgian, Victorian and Cape Dutch styles that formed a significant part of this small town. I draw attention to the ideas of dominion that Njoh elucidates, which were played out in the building of the town architectural structures. Architecture demonstrated British might and power through the imposition of British and European architectural styles on an African landscape. The sheer magnitude of the buildings, I argue, was carefully planned and the use of durable materials, often stones that were imported from abroad, was a carefully orchestrated move to demonstrate British wealth and power through intimidation and seduction. Thomas Baines was one of the artists who spent some time in Grahamstown and made a series of the landscape of this town. My interest in Baines for purposes of this thesis is the manner in which he represented Grahamstown and how he was propagator of British imperialism under the guise of ‘spreading civilisation’ among the ‘back ward’ inhabitants of this continent. My painting practice is influenced by and responds to the vacant land theory especially Baines’ works which were executed to present a Grahamstown as a purely British town ‘emptying’ it of all traces of non- British non- European dwellings or citizens. My practice brings back the layers of history that I have witnessed and the painting surface is slowly built up with water metaphorically destroying the solid structures that were built in the nineteenth century in Grahamstown. As a person who has lived through apartheid and a new dispensation in South Africa, this is reflected in my paintings with a tension between aesthetically pleasing painting styles and disturbing rough surface textures. Anselm Kiefer is the artist who has influenced my work in the manner of working he prefers and also in his tendency to look back at past periods in history.
- Full Text:
- Date Issued: 2016
- Authors: Mnyila, Desmond
- Date: 2016
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: http://hdl.handle.net/10962/4256 , vital:20639
- Description: My mini thesis is an exploration of architecture as part of the landscape of Grahamstown and how ideas of dominion and subordination of the non- white citizens of this town were asserted or communicated through space. I concur with theories about architectural buildings as objects that express power and reinforce power relations in any given society. Markus (1993) goes into great lengths to explain how buildings are primarily about power and town planning is a means of control. The area under consideration is very rich in history especially during the period that interested me which is the nineteenth century as this was a period of the establishment of Grahamstown, firstly, as a military establishment and then as a small town serving as a residential area for the British Settlers who arrived in 1820. Throughout the mini thesis I have unpacked the nature of power itself by referring to Njoh (2009) who refers to different categories of the use and especially the abuse or demonstration of power. It wouldn’t do justice to an area as rich in history as the area which is now referred to as the Albany to not dwell into some of the events that were played out here, some of which had consequences and implications for the rest of South Africa. After 1820, the town developed as more buildings of domestic houses, churches, houses of officials, prisons and schools were built. In the thesis I unpack the different architectural styles like the Georgian, Victorian and Cape Dutch styles that formed a significant part of this small town. I draw attention to the ideas of dominion that Njoh elucidates, which were played out in the building of the town architectural structures. Architecture demonstrated British might and power through the imposition of British and European architectural styles on an African landscape. The sheer magnitude of the buildings, I argue, was carefully planned and the use of durable materials, often stones that were imported from abroad, was a carefully orchestrated move to demonstrate British wealth and power through intimidation and seduction. Thomas Baines was one of the artists who spent some time in Grahamstown and made a series of the landscape of this town. My interest in Baines for purposes of this thesis is the manner in which he represented Grahamstown and how he was propagator of British imperialism under the guise of ‘spreading civilisation’ among the ‘back ward’ inhabitants of this continent. My painting practice is influenced by and responds to the vacant land theory especially Baines’ works which were executed to present a Grahamstown as a purely British town ‘emptying’ it of all traces of non- British non- European dwellings or citizens. My practice brings back the layers of history that I have witnessed and the painting surface is slowly built up with water metaphorically destroying the solid structures that were built in the nineteenth century in Grahamstown. As a person who has lived through apartheid and a new dispensation in South Africa, this is reflected in my paintings with a tension between aesthetically pleasing painting styles and disturbing rough surface textures. Anselm Kiefer is the artist who has influenced my work in the manner of working he prefers and also in his tendency to look back at past periods in history.
- Full Text:
- Date Issued: 2016
Hearing things
- Authors: Claassen, José
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6019 , http://hdl.handle.net/10962/d1021243
- Description: William Carlos Williams wrote: "It isn't what the poet says that counts as a work of art, it's what he makes, with such intensity of purpose that it lives with an intrinsic movement of its own to verify its authenticity." I would like to think that my poems only borrow life from my pen, taking on an identity and music of their own with the help of some ‘making’ on my part. My poems embrace a continuum of human experience from the intrapersonal to the societal. Using imagistic and cinematic forms, they preserve the vitality of their sources, from the music of cityscapes, to the texture of emotions, to the narratives of particular characters.
- Full Text:
- Date Issued: 2016
- Authors: Claassen, José
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6019 , http://hdl.handle.net/10962/d1021243
- Description: William Carlos Williams wrote: "It isn't what the poet says that counts as a work of art, it's what he makes, with such intensity of purpose that it lives with an intrinsic movement of its own to verify its authenticity." I would like to think that my poems only borrow life from my pen, taking on an identity and music of their own with the help of some ‘making’ on my part. My poems embrace a continuum of human experience from the intrapersonal to the societal. Using imagistic and cinematic forms, they preserve the vitality of their sources, from the music of cityscapes, to the texture of emotions, to the narratives of particular characters.
- Full Text:
- Date Issued: 2016
Movement patterns of Cape stumpnose, Rhabdosargus holubi (Sparidae), in the Kowie Estuary, South Africa
- Authors: Grant, Gareth Neil
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/982 , vital:20010
- Description: Rhabdosargus holubi is an ubiquitous estuary-dependent fishery species endemic to southern Africa. Although aspects of its recruitment dynamics from sea to estuary have received considerable research attention, little is known about its movement behaviour within estuarine systems. Fishery surveys have also revealed that R. holubi is a dominant species in catches within several temperate estuaries in South Africa. Therefore, investigating movement behaviour is fundamental to understanding the ecology of this species and providing a means for implementing effective fisheries management strategies. This study made use of passive acoustic telemetry to quantify the movement patterns of juvenile R. holubi in the Kowie Estuary, in the Eastern Cape Province of South Africa. Previous studies have revealed that surgically implanted transmitters may have adverse effects on the physiology and behaviour of tagged fish. Therefore, due to the small size of juvenile R. holubi, an a priori transmitter-effect experiment was undertaken. This experiment revealed that juvenile R. holubi (146-217 mm fork length) were not adversely affected by the surgical implantation of acoustic transmitters (7 mm Ø, 1.6 g out of water) and hence acoustic telemetry was well suited to monitoring the movements of this species. A 4-month telemetry study evaluated the movement patterns of 21 juveniles (140-190 mm fork length) tagged in three equal batches along the length of the estuary. Specific aspects of their movement included space use patterns, habitat connectivity, and the influences of geophysical cycles and environmental variables. Tagged fish exhibited high residency, which varied between each release site area. The mean length of estuary used was greater for the upper batch (15.65 ± 6.49 km) than the middle and lower batches (7.36 ± 3.68 and 2.67 ± 2 km, respectively). In terms of habitat connectivity, once the tagged fish left the estuary, they generally did not return. Tagged R. holubi spent the majority (83%) of their monitoring periods within the estuarine environment, with the sea (16%) and riverine (1%) environments being utilised to a lesser extent. Diel and tidal cycles influenced the movement patterns of juvenile R. holubi. However, the high levels of residency meant that fine-scale movements were difficult to detect due to the acoustic receiver deployment configuration (i.e. receivers moored one kilometer apart). The influence of environmental variables on the movement of individuals, modelled with general linear mixed models (GLMMs), revealed that the effects of river inflow and water temperature were greatest. Increased river inflow caused individuals to use larger portions of the estuary. Increased water temperature, particularly river temperature, caused individuals to shift their distributions downstream (towards the mouth of the estuary). This study highlighted the importance of estuarine nursery habitats to R. holubi, suggesting that a loss or reduction of healthy estuarine habitats may lead to significant declines in the abundance of this species. Furthermore, extreme resident behaviour suggests that no-take area closures (estuarine protected areas) would be an effective management strategy for this important fishery species.
- Full Text:
- Date Issued: 2016
- Authors: Grant, Gareth Neil
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/982 , vital:20010
- Description: Rhabdosargus holubi is an ubiquitous estuary-dependent fishery species endemic to southern Africa. Although aspects of its recruitment dynamics from sea to estuary have received considerable research attention, little is known about its movement behaviour within estuarine systems. Fishery surveys have also revealed that R. holubi is a dominant species in catches within several temperate estuaries in South Africa. Therefore, investigating movement behaviour is fundamental to understanding the ecology of this species and providing a means for implementing effective fisheries management strategies. This study made use of passive acoustic telemetry to quantify the movement patterns of juvenile R. holubi in the Kowie Estuary, in the Eastern Cape Province of South Africa. Previous studies have revealed that surgically implanted transmitters may have adverse effects on the physiology and behaviour of tagged fish. Therefore, due to the small size of juvenile R. holubi, an a priori transmitter-effect experiment was undertaken. This experiment revealed that juvenile R. holubi (146-217 mm fork length) were not adversely affected by the surgical implantation of acoustic transmitters (7 mm Ø, 1.6 g out of water) and hence acoustic telemetry was well suited to monitoring the movements of this species. A 4-month telemetry study evaluated the movement patterns of 21 juveniles (140-190 mm fork length) tagged in three equal batches along the length of the estuary. Specific aspects of their movement included space use patterns, habitat connectivity, and the influences of geophysical cycles and environmental variables. Tagged fish exhibited high residency, which varied between each release site area. The mean length of estuary used was greater for the upper batch (15.65 ± 6.49 km) than the middle and lower batches (7.36 ± 3.68 and 2.67 ± 2 km, respectively). In terms of habitat connectivity, once the tagged fish left the estuary, they generally did not return. Tagged R. holubi spent the majority (83%) of their monitoring periods within the estuarine environment, with the sea (16%) and riverine (1%) environments being utilised to a lesser extent. Diel and tidal cycles influenced the movement patterns of juvenile R. holubi. However, the high levels of residency meant that fine-scale movements were difficult to detect due to the acoustic receiver deployment configuration (i.e. receivers moored one kilometer apart). The influence of environmental variables on the movement of individuals, modelled with general linear mixed models (GLMMs), revealed that the effects of river inflow and water temperature were greatest. Increased river inflow caused individuals to use larger portions of the estuary. Increased water temperature, particularly river temperature, caused individuals to shift their distributions downstream (towards the mouth of the estuary). This study highlighted the importance of estuarine nursery habitats to R. holubi, suggesting that a loss or reduction of healthy estuarine habitats may lead to significant declines in the abundance of this species. Furthermore, extreme resident behaviour suggests that no-take area closures (estuarine protected areas) would be an effective management strategy for this important fishery species.
- Full Text:
- Date Issued: 2016
Meat and its meanings: representations of meat-eating in selected works of South African literature
- Authors: Coetzer, Theo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3860 , vital:20550
- Description: This thesis is situated within the burgeoning field of literary animal studies. Its aim is to analyse critically the way in which animals-as-meat are represented in South African literature. While meat pervades our lives and literature, there exists very little scholarship that considers literary depictions of meat. The thesis suggests that literary texts can offer useful reflections of the cultural environments in which they are immersed and, furthermore, can encourage what J. M. Coetzee calls the ‘sympathetic imagination’ in relation to animals. The dissertation offers close readings of three primary texts, while also drawing on a broader range of local fiction. Chapter 1 discusses Eben Venter’s Trencherman, with a specific focus on Venter’s use of the plaasroman and literary dystopia. Both genres are important to the novel’s ubiquitous depictions of meat, serving to illustrate some of the destructive, and irreversible, excesses associated with traditional Afrikaner culture in South Africa. Meat consumption is not only depicted as being among these harmful excesses, but also comes to represent them collectively. Chapter 2 offers a reading of Zakes Mda’s The Madonna of Excelsior, paying particular attention to its representation of the intersection between the objectification of women’s bodies and the transformation of animals into meat. In my approach to this text, I make use of Carol J. Adams’ notion of the ‘absent referent’. I suggest that while Mda ostensibly considers the subjugation of both women and animals, the novel does not ultimately demonstrate concern for animals in their own right. The final chapter considers the representation of suffering in Damon Galgut’s The Beautiful Screaming of Pigs. I argue that Galgut’s text is alone among the three primary texts in its attention to the animal suffering inextricably linked to meat production. The novel depicts this suffering as being comparable to human suffering, while simultaneously demonstrating humans’ indifference to their animal fellows. The dissertation concludes that while meat is infused with a range of meanings in South African literature, the most obvious and intrinsic one – the fact of animal death and animal suffering – is the one most often ignored.
- Full Text:
- Date Issued: 2016
- Authors: Coetzer, Theo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3860 , vital:20550
- Description: This thesis is situated within the burgeoning field of literary animal studies. Its aim is to analyse critically the way in which animals-as-meat are represented in South African literature. While meat pervades our lives and literature, there exists very little scholarship that considers literary depictions of meat. The thesis suggests that literary texts can offer useful reflections of the cultural environments in which they are immersed and, furthermore, can encourage what J. M. Coetzee calls the ‘sympathetic imagination’ in relation to animals. The dissertation offers close readings of three primary texts, while also drawing on a broader range of local fiction. Chapter 1 discusses Eben Venter’s Trencherman, with a specific focus on Venter’s use of the plaasroman and literary dystopia. Both genres are important to the novel’s ubiquitous depictions of meat, serving to illustrate some of the destructive, and irreversible, excesses associated with traditional Afrikaner culture in South Africa. Meat consumption is not only depicted as being among these harmful excesses, but also comes to represent them collectively. Chapter 2 offers a reading of Zakes Mda’s The Madonna of Excelsior, paying particular attention to its representation of the intersection between the objectification of women’s bodies and the transformation of animals into meat. In my approach to this text, I make use of Carol J. Adams’ notion of the ‘absent referent’. I suggest that while Mda ostensibly considers the subjugation of both women and animals, the novel does not ultimately demonstrate concern for animals in their own right. The final chapter considers the representation of suffering in Damon Galgut’s The Beautiful Screaming of Pigs. I argue that Galgut’s text is alone among the three primary texts in its attention to the animal suffering inextricably linked to meat production. The novel depicts this suffering as being comparable to human suffering, while simultaneously demonstrating humans’ indifference to their animal fellows. The dissertation concludes that while meat is infused with a range of meanings in South African literature, the most obvious and intrinsic one – the fact of animal death and animal suffering – is the one most often ignored.
- Full Text:
- Date Issued: 2016
Measuring changes in residential segregation in Port Elizabeth, South Africa, from 1996-2011
- Authors: Odayar, Luveshni
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3641 , vital:20532
- Description: Racial segregation and inequality were the prime objectives of the colonial and apartheid spatial planning policies that governed the spatial development of the South African urban landscape. Since South Africa’s democratic transition after 1994, efforts have been made by government to facilitate racial integration and transform the apartheid urban fabric. Research into post-apartheid segregation is crucial to assess the success of governmental actions enacted thus far, while informing future spatial planning strategies. Most studies of these processes have relied solely on dichotomous indices to measure the extent of segregation between two groups. Few have utilized multi-group indices, which are more suited to investigating changes in multi-ethnic cases. Furthermore, the segregation indices used are typically aspatial. This study investigates the extent to which the segregationist legacy prevails in Port Elizabeth, a place widely considered to be the founding city of urban racial segregation. It also aims to investigate the impact of geographical scale on segregation. Theil’s multi-group Entropy Index and Duncan and Duncan’s Dissimilarity Index were derived from racial population data from the 1996, 2001 and 2011 National Censuses, and GIS was used to map changes in racial diversity both at tract level and across a range of geographical scales. To generate a more holistic understanding of the observed trends, statistical tests were performed to relate the observed patterns to socio-economic determinants, and feedback from the relevant government department was obtained. The results from the entropy indices reveal that segregation levels in Port Elizabeth have declined but still remain high: 84.6 % (1996), 79.2 % (2001) to 71.5 % (2011). This is a similar trend to those found in Cape Town and Johannesburg. The integration that did occur was characterized by one-way patterns of movement into former-White areas; this was predominantly confined to the Indian population, and to a lesser extent, the Coloured population. The vast majority of the African population remains confined to their former designated location areas. The White population remains the most segregated group, despite most of the racial integration occurring in former-White areas. Interview results and statistical tests revealed that persisting racial discrepancies in socio-economic factors (income, employment and education) can be considered major drivers influencing the observed trends in racial integration and persisting desegregation, as well as self-segregation.
- Full Text:
- Date Issued: 2016
- Authors: Odayar, Luveshni
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3641 , vital:20532
- Description: Racial segregation and inequality were the prime objectives of the colonial and apartheid spatial planning policies that governed the spatial development of the South African urban landscape. Since South Africa’s democratic transition after 1994, efforts have been made by government to facilitate racial integration and transform the apartheid urban fabric. Research into post-apartheid segregation is crucial to assess the success of governmental actions enacted thus far, while informing future spatial planning strategies. Most studies of these processes have relied solely on dichotomous indices to measure the extent of segregation between two groups. Few have utilized multi-group indices, which are more suited to investigating changes in multi-ethnic cases. Furthermore, the segregation indices used are typically aspatial. This study investigates the extent to which the segregationist legacy prevails in Port Elizabeth, a place widely considered to be the founding city of urban racial segregation. It also aims to investigate the impact of geographical scale on segregation. Theil’s multi-group Entropy Index and Duncan and Duncan’s Dissimilarity Index were derived from racial population data from the 1996, 2001 and 2011 National Censuses, and GIS was used to map changes in racial diversity both at tract level and across a range of geographical scales. To generate a more holistic understanding of the observed trends, statistical tests were performed to relate the observed patterns to socio-economic determinants, and feedback from the relevant government department was obtained. The results from the entropy indices reveal that segregation levels in Port Elizabeth have declined but still remain high: 84.6 % (1996), 79.2 % (2001) to 71.5 % (2011). This is a similar trend to those found in Cape Town and Johannesburg. The integration that did occur was characterized by one-way patterns of movement into former-White areas; this was predominantly confined to the Indian population, and to a lesser extent, the Coloured population. The vast majority of the African population remains confined to their former designated location areas. The White population remains the most segregated group, despite most of the racial integration occurring in former-White areas. Interview results and statistical tests revealed that persisting racial discrepancies in socio-economic factors (income, employment and education) can be considered major drivers influencing the observed trends in racial integration and persisting desegregation, as well as self-segregation.
- Full Text:
- Date Issued: 2016
The last stop
- Authors: Mofokeng, Thabiso
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/64567 , vital:28559
- Description: My novella is set in the taxi industry. Its main characters are a wealthy taxi owner, a poor taxi driver from another African country, and the taxi driver's girlfriend. The story is partly a ghost story and partly crime fiction, it combines gritty realism with magical elements. It shows what happens between people in times of taxi violence. As the plot develops, the driver finds out that his boss is sleeping with his girlfriend. In revenge, the boss bribes some policemen to arrest the driver and beat him, and he dies in the police cells. But it turns out that the detective investigating the driver’s death is not quite impartial, nor is he of this world only.
- Full Text:
- Date Issued: 2016
- Authors: Mofokeng, Thabiso
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/64567 , vital:28559
- Description: My novella is set in the taxi industry. Its main characters are a wealthy taxi owner, a poor taxi driver from another African country, and the taxi driver's girlfriend. The story is partly a ghost story and partly crime fiction, it combines gritty realism with magical elements. It shows what happens between people in times of taxi violence. As the plot develops, the driver finds out that his boss is sleeping with his girlfriend. In revenge, the boss bribes some policemen to arrest the driver and beat him, and he dies in the police cells. But it turns out that the detective investigating the driver’s death is not quite impartial, nor is he of this world only.
- Full Text:
- Date Issued: 2016
A review of generalized linear models for count data with emphasis on current geospatial procedures
- Authors: Michell, Justin Walter
- Date: 2016
- Subjects: Spatial analysis (Statistics) , Bayesian statistical decision theory , Geospatial data , Malaria -- Botswana -- Statistics , Malaria -- Botswana -- Research -- Statistical methods
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:5582 , http://hdl.handle.net/10962/d1019989
- Description: Analytical problems caused by over-fitting, confounding and non-independence in the data is a major challenge for variable selection. As more variables are tested against a certain data set, there is a greater risk that some will explain the data merely by chance, but will fail to explain new data. The main aim of this study is to employ a systematic and practicable variable selection process for the spatial analysis and mapping of historical malaria risk in Botswana using data collected from the MARA (Mapping Malaria Risk in Africa) project and environmental and climatic datasets from various sources. Details of how a spatial database is compiled for a statistical analysis to proceed is provided. The automation of the entire process is also explored. The final bayesian spatial model derived from the non-spatial variable selection procedure using Markov Chain Monte Carlo simulation was fitted to the data. Winter temperature had the greatest effect of malaria prevalence in Botswana. Summer rainfall, maximum temperature of the warmest month, annual range of temperature, altitude and distance to closest water source were also significantly associated with malaria prevalence in the final spatial model after accounting for spatial correlation. Using this spatial model malaria prevalence at unobserved locations was predicted, producing a smooth risk map covering Botswana. The automation of both compiling the spatial database and the variable selection procedure proved challenging and could only be achieved in parts of the process. The non-spatial selection procedure proved practical and was able to identify stable explanatory variables and provide an objective means for selecting one variable over another, however ultimately it was not entirely successful due to the fact that a unique set of spatial variables could not be selected.
- Full Text:
- Date Issued: 2016
- Authors: Michell, Justin Walter
- Date: 2016
- Subjects: Spatial analysis (Statistics) , Bayesian statistical decision theory , Geospatial data , Malaria -- Botswana -- Statistics , Malaria -- Botswana -- Research -- Statistical methods
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:5582 , http://hdl.handle.net/10962/d1019989
- Description: Analytical problems caused by over-fitting, confounding and non-independence in the data is a major challenge for variable selection. As more variables are tested against a certain data set, there is a greater risk that some will explain the data merely by chance, but will fail to explain new data. The main aim of this study is to employ a systematic and practicable variable selection process for the spatial analysis and mapping of historical malaria risk in Botswana using data collected from the MARA (Mapping Malaria Risk in Africa) project and environmental and climatic datasets from various sources. Details of how a spatial database is compiled for a statistical analysis to proceed is provided. The automation of the entire process is also explored. The final bayesian spatial model derived from the non-spatial variable selection procedure using Markov Chain Monte Carlo simulation was fitted to the data. Winter temperature had the greatest effect of malaria prevalence in Botswana. Summer rainfall, maximum temperature of the warmest month, annual range of temperature, altitude and distance to closest water source were also significantly associated with malaria prevalence in the final spatial model after accounting for spatial correlation. Using this spatial model malaria prevalence at unobserved locations was predicted, producing a smooth risk map covering Botswana. The automation of both compiling the spatial database and the variable selection procedure proved challenging and could only be achieved in parts of the process. The non-spatial selection procedure proved practical and was able to identify stable explanatory variables and provide an objective means for selecting one variable over another, however ultimately it was not entirely successful due to the fact that a unique set of spatial variables could not be selected.
- Full Text:
- Date Issued: 2016
The role of leadership in shaping organisational culture in a school in Namibia.
- Authors: Nangolo, Filemon
- Date: 2016
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2075 , http://hdl.handle.net/10962/d1021303
- Description: The purpose of this study was to investigate the role of leadership in shaping the culture of a rural combined school in Oshikoto Region, northern Namibia. This school had previously performed poorly in terms of Grade 10 examination statistics for many years. However, after the appointment of a new Principal, the academic performance of the school has improved tremendously over for the past five years. Having been a teacher, Head of Department, Deputy Principal, Principal, and later an Inspector of Education, I had long held the view that the academic performance of the school depended entirely on the type of leadership the school had. Equally, I have always been aware of the negative impact of the past segregation and the discriminatory education provided by the colonial masters in South Africa and South West Africa/Namibia. The poor education provided to South Africans and the then South West Africa /Namibia posed a serious challenge to the leadership and management of schools as the majority of Principals, if not all, were poorly and inadequately trained to provide the necessary skills needed for one to be a successful Principal. Given this background, I tried to use a case study to explore the role of leadership in shaping the culture of the organisation. Research suggests that there is a strong relationship between organisational culture and school effectiveness. This study was guided by Schein’s three levels of organisational culture namely artifacts, espoused values and basic assumptions (Schein, 1992, p. 17). From these arise the following research questions: What do artifacts (symbols, structures, procedures and policies) reveal about the school culture? What are the espoused values shaping the school culture? What basic assumptions underpin the leadership of the school culture? How does leadership contribute to the creation and maintenance of a positive organisational culture at a school? The data was collected by using three different data collection methods namely, observation, document analysis and interviewing different people who hold leadership positions in the school. These were the Principal, a Head of Department, School Board chairperson, a teacher in the school management team (SMT) and a class monitor. With regard to data analysis, I first immersed myself in the data and I developed themes that formed the basis of my discussion with my research questions in mind. The analysis revealed that leadership plays a pivotal role in shaping the culture at the case study school. However, it emerged that despite the existence of a strong culture at the case study school, as with all other organisations, there are some challenges that limit the school to realise its goal of 100 % A-B symbols in all subjects.
- Full Text:
- Date Issued: 2016
- Authors: Nangolo, Filemon
- Date: 2016
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2075 , http://hdl.handle.net/10962/d1021303
- Description: The purpose of this study was to investigate the role of leadership in shaping the culture of a rural combined school in Oshikoto Region, northern Namibia. This school had previously performed poorly in terms of Grade 10 examination statistics for many years. However, after the appointment of a new Principal, the academic performance of the school has improved tremendously over for the past five years. Having been a teacher, Head of Department, Deputy Principal, Principal, and later an Inspector of Education, I had long held the view that the academic performance of the school depended entirely on the type of leadership the school had. Equally, I have always been aware of the negative impact of the past segregation and the discriminatory education provided by the colonial masters in South Africa and South West Africa/Namibia. The poor education provided to South Africans and the then South West Africa /Namibia posed a serious challenge to the leadership and management of schools as the majority of Principals, if not all, were poorly and inadequately trained to provide the necessary skills needed for one to be a successful Principal. Given this background, I tried to use a case study to explore the role of leadership in shaping the culture of the organisation. Research suggests that there is a strong relationship between organisational culture and school effectiveness. This study was guided by Schein’s three levels of organisational culture namely artifacts, espoused values and basic assumptions (Schein, 1992, p. 17). From these arise the following research questions: What do artifacts (symbols, structures, procedures and policies) reveal about the school culture? What are the espoused values shaping the school culture? What basic assumptions underpin the leadership of the school culture? How does leadership contribute to the creation and maintenance of a positive organisational culture at a school? The data was collected by using three different data collection methods namely, observation, document analysis and interviewing different people who hold leadership positions in the school. These were the Principal, a Head of Department, School Board chairperson, a teacher in the school management team (SMT) and a class monitor. With regard to data analysis, I first immersed myself in the data and I developed themes that formed the basis of my discussion with my research questions in mind. The analysis revealed that leadership plays a pivotal role in shaping the culture at the case study school. However, it emerged that despite the existence of a strong culture at the case study school, as with all other organisations, there are some challenges that limit the school to realise its goal of 100 % A-B symbols in all subjects.
- Full Text:
- Date Issued: 2016
Selfhood, identity and madness in the works of Milan Kundera and Peter Carey
- Authors: Graven, Ashley Holm
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3904 , vital:20554
- Description: Despite all the critical attention Milan Kundera’s and Peter Carey’s fiction has received, relatively little has been said about the way in which these authors problematise selfhood. In this study, I argue that these two writers share a preoccupation with the strictures placed on the individual by his/her location in language and discourse. I show that they deconstruct subjectivity with a view to intimating the possibility of momentarily transcending discursive control, and thereby inhabiting authentic selfhood. In addition, I demonstrate that both authors draw attention to the nature of language through their thematisation of madness, and I then trace the implications of this nexus between language and madness for the reader, who of course is a subject in language. My contention in this regard is that Carey and Kundera seek to instil in the reader a self-reflexive awareness of the ways in which his/her location in language shapes his/her perception of others. In turn, this awareness charges the reader with the responsibility of questioning his/her judgements, and thereby enables him/her to negotiate a measure of authenticity from his/her position in language.
- Full Text:
- Date Issued: 2016
- Authors: Graven, Ashley Holm
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3904 , vital:20554
- Description: Despite all the critical attention Milan Kundera’s and Peter Carey’s fiction has received, relatively little has been said about the way in which these authors problematise selfhood. In this study, I argue that these two writers share a preoccupation with the strictures placed on the individual by his/her location in language and discourse. I show that they deconstruct subjectivity with a view to intimating the possibility of momentarily transcending discursive control, and thereby inhabiting authentic selfhood. In addition, I demonstrate that both authors draw attention to the nature of language through their thematisation of madness, and I then trace the implications of this nexus between language and madness for the reader, who of course is a subject in language. My contention in this regard is that Carey and Kundera seek to instil in the reader a self-reflexive awareness of the ways in which his/her location in language shapes his/her perception of others. In turn, this awareness charges the reader with the responsibility of questioning his/her judgements, and thereby enables him/her to negotiate a measure of authenticity from his/her position in language.
- Full Text:
- Date Issued: 2016
The interface between nurse and patient in health care: exploring the use of emotional labour among nurses in Mthatha
- Authors: Maqabuka, Qawekzi
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/1477 , vital:20061
- Description: In exploring the use of emotional labour among nurses within the nurse/patient relationship, this study employed the conceptual framework of ‘emotional labour’ associated with Arlie Hochschild-as a means of examining the “nature” of the nurse patient interface, including the dynamics, challenges and intricacies that shape this relationship of care. The portrayal of emotional care offered to patients dealing with suffering and illness by nurses as an entirely natural activity for women is related to the devaluation of emotional labour. The focus of this study is how nurses manage their emotional involvement with patients to provide quality services. The study was conducted in Mthatha in the former Transkei in the Eastern Cape Province with nurses who worked St Mary’s Life Group and the Nelson Mandela Academic Hospital. A qualitative research design and qualitative ethnographic research methodology was chosen as suitable for answering the research question. Data was collected using semi-structured interviews and a focus group, and transcribed verbatim. Data analysis included identifying consistent emotional labour themes in the responses. The study’s main findings revealed that emotional labour strategies of surface acting and deep acting were utilised as a means of meeting organisational rules established by management of the two health care institutions that were investigated. Nurses understood that only desirable traits like include friendliness, smiling and proving a calming environment for patients should be exhibited. It was revealed that nurses often used sentimental work and emotion work in performing their tasks as this made their work easier. Lastly, the research revealed that external factors like overcrowding and shortages in personnel, accompanied by the emotional demands on nurses’ work has adverse effect on nurses work environment. The dissertation has contributed to the limited body of knowledge about emotional labour in the South African context and the lived experiences of nurses deploying their labour to patients.
- Full Text:
- Date Issued: 2016
- Authors: Maqabuka, Qawekzi
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/1477 , vital:20061
- Description: In exploring the use of emotional labour among nurses within the nurse/patient relationship, this study employed the conceptual framework of ‘emotional labour’ associated with Arlie Hochschild-as a means of examining the “nature” of the nurse patient interface, including the dynamics, challenges and intricacies that shape this relationship of care. The portrayal of emotional care offered to patients dealing with suffering and illness by nurses as an entirely natural activity for women is related to the devaluation of emotional labour. The focus of this study is how nurses manage their emotional involvement with patients to provide quality services. The study was conducted in Mthatha in the former Transkei in the Eastern Cape Province with nurses who worked St Mary’s Life Group and the Nelson Mandela Academic Hospital. A qualitative research design and qualitative ethnographic research methodology was chosen as suitable for answering the research question. Data was collected using semi-structured interviews and a focus group, and transcribed verbatim. Data analysis included identifying consistent emotional labour themes in the responses. The study’s main findings revealed that emotional labour strategies of surface acting and deep acting were utilised as a means of meeting organisational rules established by management of the two health care institutions that were investigated. Nurses understood that only desirable traits like include friendliness, smiling and proving a calming environment for patients should be exhibited. It was revealed that nurses often used sentimental work and emotion work in performing their tasks as this made their work easier. Lastly, the research revealed that external factors like overcrowding and shortages in personnel, accompanied by the emotional demands on nurses’ work has adverse effect on nurses work environment. The dissertation has contributed to the limited body of knowledge about emotional labour in the South African context and the lived experiences of nurses deploying their labour to patients.
- Full Text:
- Date Issued: 2016
Codeswitching online: a case study of a bilingual online maths programme for grade 7 learners in Diepsloot, Johannesburg
- Authors: von Witt, Nathalia
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3983 , vital:20577
- Description: There is an education crisis in South Africa. Mathematics and literacy are at the forefront of the problem, as particularly evidenced by Annual National Assessment results (Department of Basic Education, 2014; Spaull, 2014). This research is motivated by the unequal access to quality learning which stems from learners learning through a poorly-understood second language with little to no cognitive academic language proficiency. The vast majority of South African learners learn through their second language, English, from Grade 4 onwards. English is the language of South Africa’s political economy and is a global lingua franca; however, the understanding of concepts and content learnt at school is vital if one is to have any hope of putting one’s English to good use. This research aimed to find a way to equip learners both with English proficiency and mathematical understanding simultaneously. This was done by implementing and evaluating an experimental bilingual course in an existing mathematics programme in the township of Diepsloot in Johannesburg, South Africa. This research used design-based research methodology, using both qualitative and quantitative research methods. This methodology was chosen as it allows theory and practice to intersect in a real-life setting, and for the successes and shortcomings of this intersection to be evaluated. This study encompasses both the evaluation and creation of the bilingual online mathematics course. The course is made bilingual through the creation of bilingual videos with the use of translanguaging and the creation of a bilingual glossary of terms. The videos were created using a translanguaging ‘model’ informed by theories of basic interpersonal communication skills and cognitive academic language proficiency (Cummins, 1981), common underlying proficiency (Cummins, 1991), codeswitching (Setati, 1998; Ncoko et al., 2000) and translanguaging (Makalela 2015; Creese and Blackledge, 2010a). The aim of this research was to create a successful translanguaging model which facilitates learners’ ability to conceptualise in their first language and then discuss and understand the concept in their second language.
- Full Text:
- Date Issued: 2016
- Authors: von Witt, Nathalia
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3983 , vital:20577
- Description: There is an education crisis in South Africa. Mathematics and literacy are at the forefront of the problem, as particularly evidenced by Annual National Assessment results (Department of Basic Education, 2014; Spaull, 2014). This research is motivated by the unequal access to quality learning which stems from learners learning through a poorly-understood second language with little to no cognitive academic language proficiency. The vast majority of South African learners learn through their second language, English, from Grade 4 onwards. English is the language of South Africa’s political economy and is a global lingua franca; however, the understanding of concepts and content learnt at school is vital if one is to have any hope of putting one’s English to good use. This research aimed to find a way to equip learners both with English proficiency and mathematical understanding simultaneously. This was done by implementing and evaluating an experimental bilingual course in an existing mathematics programme in the township of Diepsloot in Johannesburg, South Africa. This research used design-based research methodology, using both qualitative and quantitative research methods. This methodology was chosen as it allows theory and practice to intersect in a real-life setting, and for the successes and shortcomings of this intersection to be evaluated. This study encompasses both the evaluation and creation of the bilingual online mathematics course. The course is made bilingual through the creation of bilingual videos with the use of translanguaging and the creation of a bilingual glossary of terms. The videos were created using a translanguaging ‘model’ informed by theories of basic interpersonal communication skills and cognitive academic language proficiency (Cummins, 1981), common underlying proficiency (Cummins, 1991), codeswitching (Setati, 1998; Ncoko et al., 2000) and translanguaging (Makalela 2015; Creese and Blackledge, 2010a). The aim of this research was to create a successful translanguaging model which facilitates learners’ ability to conceptualise in their first language and then discuss and understand the concept in their second language.
- Full Text:
- Date Issued: 2016
Bio-prospecting a Soil Metagenomic Library for Carbohydrate Active Esterases
- Authors: Shezi, Ntombifuthi
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4172 , http://hdl.handle.net/10962/d1021266
- Description: Lignocellulosic biomass is a promising renewable resource on earth. Plant biomass contains fermentable sugars and other moieties that can be converted to biofuels or other chemicals. Enzymatic hydrolysis of these biopolymers is significant in the liberation of sugars for fermentation into desired products. Owing to its complex structure, synergistic action of enzymes is required for its degradation. Enzymes that are involved in biomass degradation include cellulases, hemicellulases and the accessory enzymes acetyl xylan esterases and ferulic acid esterases. Ferulic acid esterases (FAEs, EC 3.1.1.73), represent a subclass of carboxylester hydrolases (EC 3.1.1.-) that catalyse the release of hydroxycinnamic acids (such as ferulic acid, p-coumaric, ferulic, sinapic and caffeic acid) that are generally found esterified to polysaccharides, such as arabinoxylans. Hydroxycinnamic acids have widespread potential applications due to their antimicrobial, photoprotectant and antioxidant properties, as well as their use as flavour precursors. Therefore, this interesting group of FAEs has a potentially wide variety of applications in agriculture, food and pharmaceutical industries. In the search for novel biocatalysts, metagenomics is considered as an alternative approach to conventional microbe screening, therefore, searching for novel biocatalysts from a soil metagenome that harbours a unique diversity of biocatalyst is significant. The aim of this study was to extract DNA from soil associated with cattle manure and construct a soil metagenomic library using a fosmid based plasmid vector and subsequently functionally screen for ferulic acid esterases using ethyl ferulate as a model substrate. A total of 59 recombinant fosmids conferring ferulic acid esterase phenotypes were identified (Hit rate 1:3122) and the two fosmids that consistently showed high FAE activities were selected for further study. Following nucleotide sequencing and translational analysis, two fae encoding open reading frames (FAE9 and FAE27) of approximately 274 and 322 aa, respectively, were identified. The amino acid sequence of the two ORFs contained a classical conserved esterase/lipase G-x-S-x-G sequence motif. The two genes (fae9 and fae27) were successfully expressed in Escherichia coli BL21 (DE3) and the purified enzymes exhibited respective temperature optima of 50 °C and 40 °C, and respective pH optima of 6.0 and 7.0. Further biochemical characterisation showed that FAE9 and FAE27 have high substrate specificity, following the fact that EFA is the preferred substrate for FAE9 (kcat/Km value of 128 s−1.mM-1) and also the preferred substrate for FAE27 (kcat/Km value of 137 s−1.mM-1). This work proves that soil is a valuable environmental source for novel esterase screening through functional based metagenomic approach. Therefore, this method may be used to screen for other valuable enzymes from environmental sources using inexpensive natural sources to encourage the screening of specific enzymes. Biochemistry of the two isolated enzymes makes these enzymes to be useful in industrial applications due to broad substrate activity that could replace the specialised enzymes to complete plant biomass degradation.
- Full Text:
- Date Issued: 2016
- Authors: Shezi, Ntombifuthi
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4172 , http://hdl.handle.net/10962/d1021266
- Description: Lignocellulosic biomass is a promising renewable resource on earth. Plant biomass contains fermentable sugars and other moieties that can be converted to biofuels or other chemicals. Enzymatic hydrolysis of these biopolymers is significant in the liberation of sugars for fermentation into desired products. Owing to its complex structure, synergistic action of enzymes is required for its degradation. Enzymes that are involved in biomass degradation include cellulases, hemicellulases and the accessory enzymes acetyl xylan esterases and ferulic acid esterases. Ferulic acid esterases (FAEs, EC 3.1.1.73), represent a subclass of carboxylester hydrolases (EC 3.1.1.-) that catalyse the release of hydroxycinnamic acids (such as ferulic acid, p-coumaric, ferulic, sinapic and caffeic acid) that are generally found esterified to polysaccharides, such as arabinoxylans. Hydroxycinnamic acids have widespread potential applications due to their antimicrobial, photoprotectant and antioxidant properties, as well as their use as flavour precursors. Therefore, this interesting group of FAEs has a potentially wide variety of applications in agriculture, food and pharmaceutical industries. In the search for novel biocatalysts, metagenomics is considered as an alternative approach to conventional microbe screening, therefore, searching for novel biocatalysts from a soil metagenome that harbours a unique diversity of biocatalyst is significant. The aim of this study was to extract DNA from soil associated with cattle manure and construct a soil metagenomic library using a fosmid based plasmid vector and subsequently functionally screen for ferulic acid esterases using ethyl ferulate as a model substrate. A total of 59 recombinant fosmids conferring ferulic acid esterase phenotypes were identified (Hit rate 1:3122) and the two fosmids that consistently showed high FAE activities were selected for further study. Following nucleotide sequencing and translational analysis, two fae encoding open reading frames (FAE9 and FAE27) of approximately 274 and 322 aa, respectively, were identified. The amino acid sequence of the two ORFs contained a classical conserved esterase/lipase G-x-S-x-G sequence motif. The two genes (fae9 and fae27) were successfully expressed in Escherichia coli BL21 (DE3) and the purified enzymes exhibited respective temperature optima of 50 °C and 40 °C, and respective pH optima of 6.0 and 7.0. Further biochemical characterisation showed that FAE9 and FAE27 have high substrate specificity, following the fact that EFA is the preferred substrate for FAE9 (kcat/Km value of 128 s−1.mM-1) and also the preferred substrate for FAE27 (kcat/Km value of 137 s−1.mM-1). This work proves that soil is a valuable environmental source for novel esterase screening through functional based metagenomic approach. Therefore, this method may be used to screen for other valuable enzymes from environmental sources using inexpensive natural sources to encourage the screening of specific enzymes. Biochemistry of the two isolated enzymes makes these enzymes to be useful in industrial applications due to broad substrate activity that could replace the specialised enzymes to complete plant biomass degradation.
- Full Text:
- Date Issued: 2016