The role of seasonality, environmental correlates and edge effects on the diversity and abundance of small mammals in Afromontane forest patches, Eastern Cape, South Africa
- Authors: Junkuhn, Kyle Peter
- Date: 2015
- Subjects: Upland ecology -- South Africa -- Eastern Cape , Mammal populations -- South Africa -- Eastern Cape , Forest animals -- Adaptation -- South Africa -- Eastern Cape , Forest animals -- Climatic factors -- South Africa -- Eastern Cape , Forest animals -- Effect of habitat modification on -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4270 , http://hdl.handle.net/10962/d1018188
- Description: The Eastern Cape contains the majority of the remaining forested areas in South Africa (95.8 percent Coastal forests and 47.6 percent Afromontane forests), however these occur in fragments. Due to the rapid rise in the human population and its needs, conversion of forests to agricultural land and the extraction of timber increases, natural vegetation are lost and this therefore leads to forests becoming fragmented into small forest patches. One of the main consequences of forest fragmentation is loss of contiguous habitat, which is the dominant threat to species globally as it negatively affects both species richness and genetic diversity. This research investigates the effects that forest fragmentation has on small mammal diversity and abundance. The study sampled small mammals in Southern Mistbelt Afromontane forest patches in the Eastern Cape. The first aim of this study was to identify which environmental variable, or combinations of variables, affect the diversity and abundance of small mammals in fragmented forest patches in the Eastern Cape during the austral summer. The second aim was to compare seasonal changes in small mammal diversity and abundance at a forest edge compared to the forest core at Beggars Bush, an Afromontane forest in the Eastern Cape, South Africa. To identify the environmental impacts that different forests have on small mammals, nine different forests were chosen in the Eastern Cape and sampled during the summer in 2013. These nine forests were: Fort Fordyce Forest, Thaba Ndoda Forest, Hogsback Forest, Dassie Kraans Forest, Langeni Forest, Burchell’s Reserve Forest, Maiden Dam Forest and Kagaberg Forest. At each site, three transects of 30 Sherman Traps each were used. Trapping periods within each forest patch lasted between three to five consecutive nights and traps were inspected once a day in the morning. Nine environmental variables were initially identified and after being tested for normality and colinearity, five variables were chosen. These variables were Altitude, Mean Annual Temperature, Gradient, Patch Size and Mean annual potential evaporation. The number of individuals captures and species richness were then incorporated as dependent variables for best subset multiple regression model selection using the Akaike Information Criterion (AIC). For the second aim, the same trapping methods were used when comparing seasonal changes to diversity and abundance within the forest core. However at the forest edge, due to the small size of the forest, only 20 Sherman traps were used in each transect with five traps (25 percent) placed in the grassland and 15 (75 percent) placed in the forest along each transect. This study was one of few to survey small mammal diversity and abundance in fragmented forests in the Eastern Cape. Gradient, patch size and mean annual potential evaporation were the variables that best predicted the individual number of small mammal captures while only gradient best explained species richness. It does however, need to be noted that capture rates and species richness were very low and this would therefore effect the analysis of environmental variables. Future studies should have a larger sample size of forest patches and include more microhabitat environmental variables to determine their effects on small mammal diversity and abundance. However, it should be noted that through climatic extinction filtering, forest mammals are resilient generalists that can tolerate fragmentation effects. Furthermore, it was found that forest edges appear to play a significant role in small mammal diversity and abundance in the Beggars Bush Afromontane forest. Some species were habitat specialists such as Rhabdomys pumilio preferring the grassland habitat and Graphiurus murinus and Aethomys namaquensis the forest habitat, while Myosorex varius was the only species that was not habitat dependant. It was found that there was a greater diversity and abundance at the forest edge compared to within the forest core throughout most of the seasons. One possible flaw was that the sampling methods were different at the edge and within the forest and therefore future studies should ensure that the method stays uniform throughout the study.
- Full Text:
- Date Issued: 2015
- Authors: Junkuhn, Kyle Peter
- Date: 2015
- Subjects: Upland ecology -- South Africa -- Eastern Cape , Mammal populations -- South Africa -- Eastern Cape , Forest animals -- Adaptation -- South Africa -- Eastern Cape , Forest animals -- Climatic factors -- South Africa -- Eastern Cape , Forest animals -- Effect of habitat modification on -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4270 , http://hdl.handle.net/10962/d1018188
- Description: The Eastern Cape contains the majority of the remaining forested areas in South Africa (95.8 percent Coastal forests and 47.6 percent Afromontane forests), however these occur in fragments. Due to the rapid rise in the human population and its needs, conversion of forests to agricultural land and the extraction of timber increases, natural vegetation are lost and this therefore leads to forests becoming fragmented into small forest patches. One of the main consequences of forest fragmentation is loss of contiguous habitat, which is the dominant threat to species globally as it negatively affects both species richness and genetic diversity. This research investigates the effects that forest fragmentation has on small mammal diversity and abundance. The study sampled small mammals in Southern Mistbelt Afromontane forest patches in the Eastern Cape. The first aim of this study was to identify which environmental variable, or combinations of variables, affect the diversity and abundance of small mammals in fragmented forest patches in the Eastern Cape during the austral summer. The second aim was to compare seasonal changes in small mammal diversity and abundance at a forest edge compared to the forest core at Beggars Bush, an Afromontane forest in the Eastern Cape, South Africa. To identify the environmental impacts that different forests have on small mammals, nine different forests were chosen in the Eastern Cape and sampled during the summer in 2013. These nine forests were: Fort Fordyce Forest, Thaba Ndoda Forest, Hogsback Forest, Dassie Kraans Forest, Langeni Forest, Burchell’s Reserve Forest, Maiden Dam Forest and Kagaberg Forest. At each site, three transects of 30 Sherman Traps each were used. Trapping periods within each forest patch lasted between three to five consecutive nights and traps were inspected once a day in the morning. Nine environmental variables were initially identified and after being tested for normality and colinearity, five variables were chosen. These variables were Altitude, Mean Annual Temperature, Gradient, Patch Size and Mean annual potential evaporation. The number of individuals captures and species richness were then incorporated as dependent variables for best subset multiple regression model selection using the Akaike Information Criterion (AIC). For the second aim, the same trapping methods were used when comparing seasonal changes to diversity and abundance within the forest core. However at the forest edge, due to the small size of the forest, only 20 Sherman traps were used in each transect with five traps (25 percent) placed in the grassland and 15 (75 percent) placed in the forest along each transect. This study was one of few to survey small mammal diversity and abundance in fragmented forests in the Eastern Cape. Gradient, patch size and mean annual potential evaporation were the variables that best predicted the individual number of small mammal captures while only gradient best explained species richness. It does however, need to be noted that capture rates and species richness were very low and this would therefore effect the analysis of environmental variables. Future studies should have a larger sample size of forest patches and include more microhabitat environmental variables to determine their effects on small mammal diversity and abundance. However, it should be noted that through climatic extinction filtering, forest mammals are resilient generalists that can tolerate fragmentation effects. Furthermore, it was found that forest edges appear to play a significant role in small mammal diversity and abundance in the Beggars Bush Afromontane forest. Some species were habitat specialists such as Rhabdomys pumilio preferring the grassland habitat and Graphiurus murinus and Aethomys namaquensis the forest habitat, while Myosorex varius was the only species that was not habitat dependant. It was found that there was a greater diversity and abundance at the forest edge compared to within the forest core throughout most of the seasons. One possible flaw was that the sampling methods were different at the edge and within the forest and therefore future studies should ensure that the method stays uniform throughout the study.
- Full Text:
- Date Issued: 2015
Case study : profitability drivers in the South African airline industry : a comparative analysis of SAA and Comair
- Authors: Batidzirai, Davison Herbert
- Date: 2015
- Subjects: South African Airways , Comair Limited , Airlines -- South Africa , Corporate profits -- South Africa , Organizational effectiveness -- South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:846 , http://hdl.handle.net/10962/d1017191
- Full Text:
- Date Issued: 2015
- Authors: Batidzirai, Davison Herbert
- Date: 2015
- Subjects: South African Airways , Comair Limited , Airlines -- South Africa , Corporate profits -- South Africa , Organizational effectiveness -- South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:846 , http://hdl.handle.net/10962/d1017191
- Full Text:
- Date Issued: 2015
Formulation, development and assessment of tenofovir disoproxil fumarate-loaded pellets
- Authors: Dube, Tawanda
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10962/54690 , vital:26600
- Description: Tenofovir disoproxil fumarate (TDF) is a novel nucleotide analog reverse transcriptase inhibitor that is recommended by the WHO for use in first line treatment of HIV infections. Due to the high dose of TDF for anti-retroviral treatment the formulation of a pellet dosage form may improve patient adherence by incorporation of a large dose in a relatively small dosage form. TDF is currently only available in tablet form. A simple, sensitive, selective, rapid, accurate, precise, stability indicating reversed-phase HPLC method was developed and validated in accordance with ICH guidelines and was successfully used for the analysis of TDF raw material and pharmaceutical dosage forms. Preformulation studies included an investigation of TDF-excipient and excipient-excipient interactions with all materials that could potentially be used to produce extruded and spheronized pellets. Nuclear Magnetic Resonance spectroscopy (NMR), Infrared Spectroscopy (IR), Differential Scanning Colorimetry (DSC) and Thermogravimetric analysis were used for identification and purity testing of TDF and all excipients. DSC data revealed that no potential interactions between TDF and the excipients occurred suggesting that incompatibility reactions were unlikely during manufacture and storage. These findings were confirmed by IR analysis that revealed that no physical interaction was likely between any of the excipients used and TDF. DSC data also reveal the existence of the α and β-polymorphs of TDF as evidenced by two enthalpy changes observed on the resultant thermograms. The existence of two polymorphs is unlikely to result in incompatibility and was confirmed by IR analysis. The IR spectra reveal that all characteristic peaks for TDF were present in 1:1 binary mixtures. Therefore TDF is compatible with all excipients tested and thermal analysis confirmed the stability of TDF under manufacturing conditions. The temperature of degradation temperature established through DSC analysis confirmed that degradation during manufacture is unlikely as the temperature of manufacture is lower than that at which degradation occurs. Extrusion and spheronization were the processes used to manufacture TDF pellets as it is a simple and economic approach for production. The effects of extruder and spheronizer speed, amount of spheronization aid and diluents on the pellet size, shape, flow properties and TDF release characteristics were examined. In order to decrease the complexity of analysis and reduce the cost of development a Taguchi orthogonal array design of experiments was successfully applied to evaluate the impact of formulation variables on product characteristics and predict an optimized formulation with a minimum number of experiments. The use of Response Surface Methodology for the development and optimization of pharmaceutical systems, including the optimization of formulation composition, manufacturing processes and/or analytical methods is well established. However the application of RSM requires that accurate, precise and reproducible experimental conditions are used for the generation of reliable data and RSM use is limited due to sensitivity to experimental variability. The benefits of using RSM for formulation optimization include the fact that more than one variable can be investigated at a time and large amounts of information can be generated at the same time ensuring a more efficient process with respect to time and cost. An added advantage of this approach is that mathematical relationships can be generated for the models that are produced and provide formulation scientists with an indication of whether the effect(s) between factors are synergistic or antagonistic. There are several statistical design approaches that use RSM and a Taguchi orthogonal array design was selected for use in this optimization process as fewer experiments are required to generate data for the same number of factors to be investigated when compared to other statistical designs such as Central Composite (CCD) and Box-Behnken designs. The use of RSM clearly demonstrates the impact of different input variables on the % TDF released at 45 min and % TDF loaded into the particles. The amount of sorbitol and Kollidon® CL-M were the only significant variables that affected the % TDF released at 45 min and both excipients had an overall synergistic effect on the in vitro release of TDF. The prediction and manufacture of an optimized formulation led to the production of pellets that met predetermined specifications which was successfully achieved using RSM. The development of a TDF containing pellet dosage form has been achieved and the formulation, manufacture and characterization of the dosage form reveal that the product has the potential to be further developed.
- Full Text:
- Date Issued: 2015
- Authors: Dube, Tawanda
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10962/54690 , vital:26600
- Description: Tenofovir disoproxil fumarate (TDF) is a novel nucleotide analog reverse transcriptase inhibitor that is recommended by the WHO for use in first line treatment of HIV infections. Due to the high dose of TDF for anti-retroviral treatment the formulation of a pellet dosage form may improve patient adherence by incorporation of a large dose in a relatively small dosage form. TDF is currently only available in tablet form. A simple, sensitive, selective, rapid, accurate, precise, stability indicating reversed-phase HPLC method was developed and validated in accordance with ICH guidelines and was successfully used for the analysis of TDF raw material and pharmaceutical dosage forms. Preformulation studies included an investigation of TDF-excipient and excipient-excipient interactions with all materials that could potentially be used to produce extruded and spheronized pellets. Nuclear Magnetic Resonance spectroscopy (NMR), Infrared Spectroscopy (IR), Differential Scanning Colorimetry (DSC) and Thermogravimetric analysis were used for identification and purity testing of TDF and all excipients. DSC data revealed that no potential interactions between TDF and the excipients occurred suggesting that incompatibility reactions were unlikely during manufacture and storage. These findings were confirmed by IR analysis that revealed that no physical interaction was likely between any of the excipients used and TDF. DSC data also reveal the existence of the α and β-polymorphs of TDF as evidenced by two enthalpy changes observed on the resultant thermograms. The existence of two polymorphs is unlikely to result in incompatibility and was confirmed by IR analysis. The IR spectra reveal that all characteristic peaks for TDF were present in 1:1 binary mixtures. Therefore TDF is compatible with all excipients tested and thermal analysis confirmed the stability of TDF under manufacturing conditions. The temperature of degradation temperature established through DSC analysis confirmed that degradation during manufacture is unlikely as the temperature of manufacture is lower than that at which degradation occurs. Extrusion and spheronization were the processes used to manufacture TDF pellets as it is a simple and economic approach for production. The effects of extruder and spheronizer speed, amount of spheronization aid and diluents on the pellet size, shape, flow properties and TDF release characteristics were examined. In order to decrease the complexity of analysis and reduce the cost of development a Taguchi orthogonal array design of experiments was successfully applied to evaluate the impact of formulation variables on product characteristics and predict an optimized formulation with a minimum number of experiments. The use of Response Surface Methodology for the development and optimization of pharmaceutical systems, including the optimization of formulation composition, manufacturing processes and/or analytical methods is well established. However the application of RSM requires that accurate, precise and reproducible experimental conditions are used for the generation of reliable data and RSM use is limited due to sensitivity to experimental variability. The benefits of using RSM for formulation optimization include the fact that more than one variable can be investigated at a time and large amounts of information can be generated at the same time ensuring a more efficient process with respect to time and cost. An added advantage of this approach is that mathematical relationships can be generated for the models that are produced and provide formulation scientists with an indication of whether the effect(s) between factors are synergistic or antagonistic. There are several statistical design approaches that use RSM and a Taguchi orthogonal array design was selected for use in this optimization process as fewer experiments are required to generate data for the same number of factors to be investigated when compared to other statistical designs such as Central Composite (CCD) and Box-Behnken designs. The use of RSM clearly demonstrates the impact of different input variables on the % TDF released at 45 min and % TDF loaded into the particles. The amount of sorbitol and Kollidon® CL-M were the only significant variables that affected the % TDF released at 45 min and both excipients had an overall synergistic effect on the in vitro release of TDF. The prediction and manufacture of an optimized formulation led to the production of pellets that met predetermined specifications which was successfully achieved using RSM. The development of a TDF containing pellet dosage form has been achieved and the formulation, manufacture and characterization of the dosage form reveal that the product has the potential to be further developed.
- Full Text:
- Date Issued: 2015
The Ecca type section (Permian, South Africa) : an outcrop analogue study of conventional and unconventional hydrocarbon reservoirs
- Authors: Campbell, Stuart Alexander
- Date: 2015
- Subjects: Hydrocarbon reservoirs -- Ecca Group , Geology, Stratigraphic -- Permian , Paleogeography -- Ecca Group , Shale gas -- Ecca Group , Shale gas reservoirs -- Ecca Group , Ecca Group , Ripon Formation (South Africa) , Collingham Formation (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5083 , http://hdl.handle.net/10962/d1018199
- Description: The Karoo Basin of South Africa holds an estimated 906 billion to 11 trillion cubic meters of unconventional shale gas within the shales of the Whitehill and Collingham formations of the Ecca Group. Evaluation of this potential resource has been limited due to the lack of exploration and a scarcity of existing drill core data. In order to circumnavigate this problem this study was undertaken to evaluate the potential target horizons exposed in outcrops along the southern portion of the Karoo Basin, north of Grahamstown in the Eastern Cape Province. Detailed field logging was done on the exposed Whitehill and Collingham formations as well as a possible conventional sandstone (turbidite) reservoir, the Ripon Formation, along road cuttings of the Ecca Pass. Palaeocurrent data, jointing directions and fossil material were also documented. Samples were analysed for mineralogy, porosity, permeability, and total organic carbon content (TOC). The extensively weathered black shales of the Whitehill Formation contain a maximum TOC value of 0.9% and the Collingham Formation shales contain a maximum TOC value of 0.6%. The organic lithic arkose sandstones of the Ripon Formation are classified as ‘tight rock’ with an average porosity of 1% and an average permeability of 0.05 mD. The Whitehill Formation in the southern portion of the Karoo Basin has experienced organic matter loss due to low grade metamorphism as well as burial to extreme depths, thus reducing shale gas potential. The Ripon Formation is an unsuitable conventional reservoir along the southern basin boundary due to extensive cementation and filling of pore spaces.
- Full Text:
- Date Issued: 2015
- Authors: Campbell, Stuart Alexander
- Date: 2015
- Subjects: Hydrocarbon reservoirs -- Ecca Group , Geology, Stratigraphic -- Permian , Paleogeography -- Ecca Group , Shale gas -- Ecca Group , Shale gas reservoirs -- Ecca Group , Ecca Group , Ripon Formation (South Africa) , Collingham Formation (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5083 , http://hdl.handle.net/10962/d1018199
- Description: The Karoo Basin of South Africa holds an estimated 906 billion to 11 trillion cubic meters of unconventional shale gas within the shales of the Whitehill and Collingham formations of the Ecca Group. Evaluation of this potential resource has been limited due to the lack of exploration and a scarcity of existing drill core data. In order to circumnavigate this problem this study was undertaken to evaluate the potential target horizons exposed in outcrops along the southern portion of the Karoo Basin, north of Grahamstown in the Eastern Cape Province. Detailed field logging was done on the exposed Whitehill and Collingham formations as well as a possible conventional sandstone (turbidite) reservoir, the Ripon Formation, along road cuttings of the Ecca Pass. Palaeocurrent data, jointing directions and fossil material were also documented. Samples were analysed for mineralogy, porosity, permeability, and total organic carbon content (TOC). The extensively weathered black shales of the Whitehill Formation contain a maximum TOC value of 0.9% and the Collingham Formation shales contain a maximum TOC value of 0.6%. The organic lithic arkose sandstones of the Ripon Formation are classified as ‘tight rock’ with an average porosity of 1% and an average permeability of 0.05 mD. The Whitehill Formation in the southern portion of the Karoo Basin has experienced organic matter loss due to low grade metamorphism as well as burial to extreme depths, thus reducing shale gas potential. The Ripon Formation is an unsuitable conventional reservoir along the southern basin boundary due to extensive cementation and filling of pore spaces.
- Full Text:
- Date Issued: 2015
NGOs and the depoliticisation of development : the case of GADRA education in Grahamstown
- Authors: Nqaba, Patronella Pinky
- Date: 2015
- Subjects: GADRA Education (Grahamstown, South Africa) , Non-governmental organizations -- South Africa -- Grahamstown , Non-governmental organizations -- Political aspects , Education -- South Africa -- History , Right to education -- South Africa , Educational change -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2890 , http://hdl.handle.net/10962/d1017865
- Description: Non-governmental organisations (NGOs) have been criticised for depoliticising development through focusing on alleviating suffering rather than on addressing the root causes of poverty and underdevelopment. This research explores whether and how NGOs can act in ways that do not depoliticise development. The research focuses on education NGOs and in particular on the NGO GADRA education in Grahamstown, South Africa, to provide insights into ways in which politically conscious leadership of NGOs attempt to deal with the contradictions that are inherent in this field of work. This research provides a brief history of the South African Education system as a means to set a basis for the discussion of the role of education NGOs in the country. Furthermore it looks at the work that is done by GADRA education in the Grahamstown community. The thesis makes the argument that education NGOs can act in ways that do not depoliticise development because by providing access to education for people who are structurally excluded from education, they contribute to shifting power. This research found that although the leadership of GADRA Education acknowledge that they are confronted with great challenges in terms of how to bring about changes in the education system, they are hopeful that advocating for quality education will bring about the potential for the disruption of power relations as they exist between the state organs and the public.
- Full Text:
- Date Issued: 2015
- Authors: Nqaba, Patronella Pinky
- Date: 2015
- Subjects: GADRA Education (Grahamstown, South Africa) , Non-governmental organizations -- South Africa -- Grahamstown , Non-governmental organizations -- Political aspects , Education -- South Africa -- History , Right to education -- South Africa , Educational change -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2890 , http://hdl.handle.net/10962/d1017865
- Description: Non-governmental organisations (NGOs) have been criticised for depoliticising development through focusing on alleviating suffering rather than on addressing the root causes of poverty and underdevelopment. This research explores whether and how NGOs can act in ways that do not depoliticise development. The research focuses on education NGOs and in particular on the NGO GADRA education in Grahamstown, South Africa, to provide insights into ways in which politically conscious leadership of NGOs attempt to deal with the contradictions that are inherent in this field of work. This research provides a brief history of the South African Education system as a means to set a basis for the discussion of the role of education NGOs in the country. Furthermore it looks at the work that is done by GADRA education in the Grahamstown community. The thesis makes the argument that education NGOs can act in ways that do not depoliticise development because by providing access to education for people who are structurally excluded from education, they contribute to shifting power. This research found that although the leadership of GADRA Education acknowledge that they are confronted with great challenges in terms of how to bring about changes in the education system, they are hopeful that advocating for quality education will bring about the potential for the disruption of power relations as they exist between the state organs and the public.
- Full Text:
- Date Issued: 2015
The role of civil society in advancing education rights : the case of Gadra Education, Grahamstown, South Africa
- Authors: Msindo, Esteri Makotore
- Date: 2015
- Subjects: Gadra Education (Grahamstown, South Africa) , Right to education -- South Africa , State departments of education -- South Africa , Educational change -- South Africa , Social justice -- South Africa , Civil society -- South Africa , Social contract
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3392 , http://hdl.handle.net/10962/d1016500
- Description: This thesis has identified and analysed the role of an NGO called Gadra Education in advancing education rights to the less advantaged people of Grahamstown in South Africa. Gadra Education’s role has been identified as twofold. Firstly as an educational NGO, Gadra Education’s initiatives directly impact on the lives of the less economically and socially privileged learners who, due to their previous learning environment in state schools, do not achieve academic results that ensure entry into tertiary level. Secondly its role is identified in its nature as an organisation that emerged due to the deficiencies in the state schooling system. It therefore stands de facto as a critical institution for critique of the state’s education system. The thesis concludes that without confronting the Department of Education or collaborating with it, Gadra Education offers a significant alternative approach which can potentially influence the state to improve the state schooling system. Its strategy of non-confrontation to the state, informal and non-corporatist is advantageous as an NGO that focuses on the actual provision of education. It focuses on instilling Ubuntu values of sharing and giving that are of critical significance in teaching and learning. The context of the thesis is located broadly within socio-economic rights and specifically on education rights. In South Africa where the state has not adequately met the educational obligations for the economically and socially less privileged citizens, the emergence of educational NGOs that focus on providing education to the poor is of vital importance. Although other NGOs that confront the state are important in pushing the state to deliver especially on school infrastructure, teacher deployment and other educational challenges, Gadra Education model ensures academic success for the learner. Lessons can be drawn from Gadra Education which can be potentially useful to state schools and other NGOs that seek to advance education rights to disadvantaged communities.
- Full Text:
- Date Issued: 2015
- Authors: Msindo, Esteri Makotore
- Date: 2015
- Subjects: Gadra Education (Grahamstown, South Africa) , Right to education -- South Africa , State departments of education -- South Africa , Educational change -- South Africa , Social justice -- South Africa , Civil society -- South Africa , Social contract
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3392 , http://hdl.handle.net/10962/d1016500
- Description: This thesis has identified and analysed the role of an NGO called Gadra Education in advancing education rights to the less advantaged people of Grahamstown in South Africa. Gadra Education’s role has been identified as twofold. Firstly as an educational NGO, Gadra Education’s initiatives directly impact on the lives of the less economically and socially privileged learners who, due to their previous learning environment in state schools, do not achieve academic results that ensure entry into tertiary level. Secondly its role is identified in its nature as an organisation that emerged due to the deficiencies in the state schooling system. It therefore stands de facto as a critical institution for critique of the state’s education system. The thesis concludes that without confronting the Department of Education or collaborating with it, Gadra Education offers a significant alternative approach which can potentially influence the state to improve the state schooling system. Its strategy of non-confrontation to the state, informal and non-corporatist is advantageous as an NGO that focuses on the actual provision of education. It focuses on instilling Ubuntu values of sharing and giving that are of critical significance in teaching and learning. The context of the thesis is located broadly within socio-economic rights and specifically on education rights. In South Africa where the state has not adequately met the educational obligations for the economically and socially less privileged citizens, the emergence of educational NGOs that focus on providing education to the poor is of vital importance. Although other NGOs that confront the state are important in pushing the state to deliver especially on school infrastructure, teacher deployment and other educational challenges, Gadra Education model ensures academic success for the learner. Lessons can be drawn from Gadra Education which can be potentially useful to state schools and other NGOs that seek to advance education rights to disadvantaged communities.
- Full Text:
- Date Issued: 2015
The effects of labour law on small firms in South Africa : perceptions of employers in the hospitality sector in Pretoria, Gauteng
- Authors: MacNeill, Jessica Dawn
- Date: 2015
- Subjects: Labor laws and legislation -- South Africa , Small business -- South Africa -- Pretoria , Small business -- South Africa -- Pretoria -- Personnel management , Hospitality industry -- South Africa -- Pretoria -- Personnel management , Manpower policy -- South Africa
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3405 , http://hdl.handle.net/10962/d1018934
- Description: The South African government has attempted to find a balance of interests between the employer and the employee by the introduction of the Labour Relations Act in 1995 and the Basic Conditions of Employment Act in 1997. It is critical to the health of the South African economy that these labour laws do not impact small businesses to the extent that the Gross Domestic Product of the country is negatively affected. There are conflicting reports as to how these labour laws affect small businesses. It is therefore important for government to be able to understand, define and measure the impact of its labour laws on small businesses, in order for it to strategise corrective measures, which may include reconsidering the application of the legislative directive, regulated flexibility, if required. The study was limited in the sense that it was solely based on evidence collected from employers. An interpretivist approach was applied as a research methodology to data collected through in-depth interviews. The main findings of the empirical analysis demonstrate that labour legislation does not heavily impact small firms. It was thus determined that extensive measures were not needed with regard to correcting the framework of regulated flexibility.
- Full Text:
- Date Issued: 2015
- Authors: MacNeill, Jessica Dawn
- Date: 2015
- Subjects: Labor laws and legislation -- South Africa , Small business -- South Africa -- Pretoria , Small business -- South Africa -- Pretoria -- Personnel management , Hospitality industry -- South Africa -- Pretoria -- Personnel management , Manpower policy -- South Africa
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3405 , http://hdl.handle.net/10962/d1018934
- Description: The South African government has attempted to find a balance of interests between the employer and the employee by the introduction of the Labour Relations Act in 1995 and the Basic Conditions of Employment Act in 1997. It is critical to the health of the South African economy that these labour laws do not impact small businesses to the extent that the Gross Domestic Product of the country is negatively affected. There are conflicting reports as to how these labour laws affect small businesses. It is therefore important for government to be able to understand, define and measure the impact of its labour laws on small businesses, in order for it to strategise corrective measures, which may include reconsidering the application of the legislative directive, regulated flexibility, if required. The study was limited in the sense that it was solely based on evidence collected from employers. An interpretivist approach was applied as a research methodology to data collected through in-depth interviews. The main findings of the empirical analysis demonstrate that labour legislation does not heavily impact small firms. It was thus determined that extensive measures were not needed with regard to correcting the framework of regulated flexibility.
- Full Text:
- Date Issued: 2015
Structure of the nucleus ¹¹⁴Sn using gamma-ray coincidence data
- Authors: Oates, Sean Benjamin
- Date: 2015
- Subjects: High spin physics , Nuclear structure , Nuclear shell theory , Neutron counters , Decay schemes (Radioactivity) , Coincidence circuits , Collective excitations , Anisotropy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5562 , http://hdl.handle.net/10962/d1019870
- Full Text:
- Date Issued: 2015
- Authors: Oates, Sean Benjamin
- Date: 2015
- Subjects: High spin physics , Nuclear structure , Nuclear shell theory , Neutron counters , Decay schemes (Radioactivity) , Coincidence circuits , Collective excitations , Anisotropy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5562 , http://hdl.handle.net/10962/d1019870
- Full Text:
- Date Issued: 2015
The interrelationships between foreign direct investment and economic growth in Africa
- Authors: Bolani, Lindelwa Mandisa
- Date: 2015
- Subjects: Investments, Foreign -- Africa , Economic development -- Africa , Africa -- Economic conditions -- 1960- , Africa -- Foreign economic relations , Gross domestic product -- Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1123 , http://hdl.handle.net/10962/d1019885
- Description: There has been a long search for the keys to development and economic growth in Africa. This study investigates the relationship between FDI and economic growth over the period 2000-2012 using data from 48 African countries. On the aggregate regional level FDI and economic growth were found to be positively correlated during this period. Using panel data econometric techniques and the Panel Granger Causality test, results revealed that a bi-directional causality relationship existed between FDI and GDP. Thus, the results suggest that GDP is a requirement for increased investment, and at the same time is the result of increased foreign investment. Thus, the conclusion is that African policy makers are justified in increasing their attempts to create an attractive business environment for foreign investors, as it is beneficial for economic growth.
- Full Text:
- Date Issued: 2015
- Authors: Bolani, Lindelwa Mandisa
- Date: 2015
- Subjects: Investments, Foreign -- Africa , Economic development -- Africa , Africa -- Economic conditions -- 1960- , Africa -- Foreign economic relations , Gross domestic product -- Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1123 , http://hdl.handle.net/10962/d1019885
- Description: There has been a long search for the keys to development and economic growth in Africa. This study investigates the relationship between FDI and economic growth over the period 2000-2012 using data from 48 African countries. On the aggregate regional level FDI and economic growth were found to be positively correlated during this period. Using panel data econometric techniques and the Panel Granger Causality test, results revealed that a bi-directional causality relationship existed between FDI and GDP. Thus, the results suggest that GDP is a requirement for increased investment, and at the same time is the result of increased foreign investment. Thus, the conclusion is that African policy makers are justified in increasing their attempts to create an attractive business environment for foreign investors, as it is beneficial for economic growth.
- Full Text:
- Date Issued: 2015
Link between ghost artefacts, source suppression and incomplete calibration sky models
- Authors: Nunhokee, Chuneeta Devi
- Date: 2015
- Subjects: Interferometry , Calibration
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5556 , http://hdl.handle.net/10962/d1017900
- Description: Calibration is a fundamental step towards producing radio interferometric images. However, naive calibration produces calibration artefacts, in the guise of spurious emission, buried in the thermal noise. This work investigates these calibration artefacts, henceforth referred to as “ghosts”. A 21 cm observation with the Westerbork Synthesis Radio Telescope yielded similar ghost sources, and it was anticipated that they were due to calibrating with incomplete sky models. An analytical ghost distribution of a two-source scenario is derived to substantiate this theory and to seek answers to the related bewildering features (regular ghost pattern, points spread function-like sidelobes, independent of model flux). The theoretically predicted ghost distribution qualitatively matches with the observational ones and shows high dependence on the array geometry. The theory draws the conclusion that both the ghost phenomenon and suppression of the unmodelled flux have the same root cause. In addition, the suppression of the unmodelled flux is studied as functions of unmodelled flux, differential gain solution interval and the number of sources subjected to direction-dependent gains. These studies summarise that the suppression rate is constant irrespective of the degree of incompleteness of the calibration sky model. In the presence of a direction-dependent effect, the suppression drastically increases; however, this increase can be compensated for by using longer solution intervals.
- Full Text:
- Date Issued: 2015
- Authors: Nunhokee, Chuneeta Devi
- Date: 2015
- Subjects: Interferometry , Calibration
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5556 , http://hdl.handle.net/10962/d1017900
- Description: Calibration is a fundamental step towards producing radio interferometric images. However, naive calibration produces calibration artefacts, in the guise of spurious emission, buried in the thermal noise. This work investigates these calibration artefacts, henceforth referred to as “ghosts”. A 21 cm observation with the Westerbork Synthesis Radio Telescope yielded similar ghost sources, and it was anticipated that they were due to calibrating with incomplete sky models. An analytical ghost distribution of a two-source scenario is derived to substantiate this theory and to seek answers to the related bewildering features (regular ghost pattern, points spread function-like sidelobes, independent of model flux). The theoretically predicted ghost distribution qualitatively matches with the observational ones and shows high dependence on the array geometry. The theory draws the conclusion that both the ghost phenomenon and suppression of the unmodelled flux have the same root cause. In addition, the suppression of the unmodelled flux is studied as functions of unmodelled flux, differential gain solution interval and the number of sources subjected to direction-dependent gains. These studies summarise that the suppression rate is constant irrespective of the degree of incompleteness of the calibration sky model. In the presence of a direction-dependent effect, the suppression drastically increases; however, this increase can be compensated for by using longer solution intervals.
- Full Text:
- Date Issued: 2015
Performance management of health professionals : an evaluation research study of health services in the subdistrict of Buffalo City in the Provincial Department of Health in the Eastern Cape
- Authors: Booi, Mlungisi Wellington
- Date: 2015
- Subjects: South Africa -- Department of Health , Batho Pele (Program) , Public health personnel -- South Africa -- Buffalo City , Employees -- Rating of -- South Africa -- Buffalo City , Public health -- South Africa -- Buffalo City -- Evaluation , Customer services -- South Africa -- Buffalo City -- Evaluation , Employees -- Training of -- South Africa -- Buffalo City , Employee-management relations in government -- South Africa -- Buffalo City
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:859 , http://hdl.handle.net/10962/d1020017
- Description: Integrative Summary In 1997 the Minister of Public Service and Administration of South Africa, Zola Skweyiya, introduced the White Paper on Transforming Public Service Delivery(Department of Public Service and Administration(DPSA,1997: 1). It was premised on what was called Batho Pele (DPSA, 1997: 9), giving effect to Section 197 of the Constitution, Act 108 of 1996 as amended. A linkage to performance management can be found from the assertion made in the White Paper that in future the Batho Pele would form the basis of any assessment of the performance of individual staff and that would contribute to improving the delivery of service (DPSA, 1997:16). Another factor that contributed to the utilisation of a performance management system was the recommendation put forward by the Public Service Commission of South Africa( PSC,2004: 16),that called for public institutions to adopt a clear, comprehensive performance management and evaluation framework for the delivery of public service. That was expected to turn around the ailing public institutions whose record of serving the public with pride and dignity was on the decline. The research aim was to evaluate the effectiveness of the performance management system in creating and resolving role conflict with health professionals employed by the Buffalo City sub-district. The research goal was divided into three objectives, namely: evaluation of the effectiveness of the performance management system as a managerial tool for health managers to manage their subordinates including both professional and support staff, evaluation of the effectiveness of the performance system from the perspective of the health professional as a subordinate and to analyze the functioning and effectiveness of the system in creating and resolving role conflict between professional conduct and organisational requirements. The respondents noted that the implementation of the system was not matched with appointment at managerial level with officials having relevant managerial capacity. Further to that resources at the coal face of service delivery continued to deteriorate and became very scarce and there is no documented proof that has been found of an effort made to check the compatibility of the system to health professionals. The literature identified negative effects that have emanated from the implementation of the performance management system. The study was conducted by interviewing health professionals from the entry level to the management level within the Buffalo City sub-district from three mini hospitals or Health Care Centres. The respondents indicated that there are positives that have been achieved by the implementation of the system, such as the skills gap identification as well as specific targets contained in annual performance contracts. These targets help motivate health professional to focus on that particular direction of activities. Unfortunately it has been clearly documented that the performance management system has also contributed to the development of role conflict in a number of professionals. It has been documented that the system has not been crafted with a health professional in mind and, as such, appears to favour quantity rather than quality of health services as advocated by the codes of practice of different health professionals. There are a number of recommendations that were put forward by the respondents to try to salvage the system and in mitigation of the identified negative factors. For the improvement of the system, the Eastern Cape Provincial Department of Health has to firstly customise the annual contracts to contain only the fundamental information. Secondly need to improve the transparency pertaining to financial pressures facing the Health Department. Thirdly the provision of essential enablers, need to be prioritised. Fourthly there is a need to upgrade the system to incorporate 360 degree feedback. Further research recommendations include a bigger sample incorporating different research methods and to also incorporate searches for measures that can be implemented to improve the system to be more relevant to health professionals. This document is organised and presented in three sections. The first section is the evaluation report with a review of literature, research methodology, findings, discussion and conclusion. The second section is where an indepth literature review is located and the last section details the research methodology.
- Full Text:
- Date Issued: 2015
- Authors: Booi, Mlungisi Wellington
- Date: 2015
- Subjects: South Africa -- Department of Health , Batho Pele (Program) , Public health personnel -- South Africa -- Buffalo City , Employees -- Rating of -- South Africa -- Buffalo City , Public health -- South Africa -- Buffalo City -- Evaluation , Customer services -- South Africa -- Buffalo City -- Evaluation , Employees -- Training of -- South Africa -- Buffalo City , Employee-management relations in government -- South Africa -- Buffalo City
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:859 , http://hdl.handle.net/10962/d1020017
- Description: Integrative Summary In 1997 the Minister of Public Service and Administration of South Africa, Zola Skweyiya, introduced the White Paper on Transforming Public Service Delivery(Department of Public Service and Administration(DPSA,1997: 1). It was premised on what was called Batho Pele (DPSA, 1997: 9), giving effect to Section 197 of the Constitution, Act 108 of 1996 as amended. A linkage to performance management can be found from the assertion made in the White Paper that in future the Batho Pele would form the basis of any assessment of the performance of individual staff and that would contribute to improving the delivery of service (DPSA, 1997:16). Another factor that contributed to the utilisation of a performance management system was the recommendation put forward by the Public Service Commission of South Africa( PSC,2004: 16),that called for public institutions to adopt a clear, comprehensive performance management and evaluation framework for the delivery of public service. That was expected to turn around the ailing public institutions whose record of serving the public with pride and dignity was on the decline. The research aim was to evaluate the effectiveness of the performance management system in creating and resolving role conflict with health professionals employed by the Buffalo City sub-district. The research goal was divided into three objectives, namely: evaluation of the effectiveness of the performance management system as a managerial tool for health managers to manage their subordinates including both professional and support staff, evaluation of the effectiveness of the performance system from the perspective of the health professional as a subordinate and to analyze the functioning and effectiveness of the system in creating and resolving role conflict between professional conduct and organisational requirements. The respondents noted that the implementation of the system was not matched with appointment at managerial level with officials having relevant managerial capacity. Further to that resources at the coal face of service delivery continued to deteriorate and became very scarce and there is no documented proof that has been found of an effort made to check the compatibility of the system to health professionals. The literature identified negative effects that have emanated from the implementation of the performance management system. The study was conducted by interviewing health professionals from the entry level to the management level within the Buffalo City sub-district from three mini hospitals or Health Care Centres. The respondents indicated that there are positives that have been achieved by the implementation of the system, such as the skills gap identification as well as specific targets contained in annual performance contracts. These targets help motivate health professional to focus on that particular direction of activities. Unfortunately it has been clearly documented that the performance management system has also contributed to the development of role conflict in a number of professionals. It has been documented that the system has not been crafted with a health professional in mind and, as such, appears to favour quantity rather than quality of health services as advocated by the codes of practice of different health professionals. There are a number of recommendations that were put forward by the respondents to try to salvage the system and in mitigation of the identified negative factors. For the improvement of the system, the Eastern Cape Provincial Department of Health has to firstly customise the annual contracts to contain only the fundamental information. Secondly need to improve the transparency pertaining to financial pressures facing the Health Department. Thirdly the provision of essential enablers, need to be prioritised. Fourthly there is a need to upgrade the system to incorporate 360 degree feedback. Further research recommendations include a bigger sample incorporating different research methods and to also incorporate searches for measures that can be implemented to improve the system to be more relevant to health professionals. This document is organised and presented in three sections. The first section is the evaluation report with a review of literature, research methodology, findings, discussion and conclusion. The second section is where an indepth literature review is located and the last section details the research methodology.
- Full Text:
- Date Issued: 2015
What limits an invasive biotic and abiotic effects on the distribution of the invasive mussel mytilus galloprovincialis on the South African coastline
- Authors: Hall, Madison
- Date: 2015
- Subjects: Perna , Mytilus galloprovincialis , Mussels -- South Africa , Introduced organisms -- Environmental aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5927 , http://hdl.handle.net/10962/d1017805
- Full Text:
- Date Issued: 2015
- Authors: Hall, Madison
- Date: 2015
- Subjects: Perna , Mytilus galloprovincialis , Mussels -- South Africa , Introduced organisms -- Environmental aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5927 , http://hdl.handle.net/10962/d1017805
- Full Text:
- Date Issued: 2015
Teacher leadership : a study in a township high school
- Authors: Kumalo, Elizabeth Nomso
- Date: 2015
- Subjects: Educational leadership -- South Africa -- Eastern Cape , Teacher participation in administration -- South Africa -- Eastern Cape , Teacher effectiveness -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2038 , http://hdl.handle.net/10962/d1017349
- Full Text:
- Date Issued: 2015
- Authors: Kumalo, Elizabeth Nomso
- Date: 2015
- Subjects: Educational leadership -- South Africa -- Eastern Cape , Teacher participation in administration -- South Africa -- Eastern Cape , Teacher effectiveness -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2038 , http://hdl.handle.net/10962/d1017349
- Full Text:
- Date Issued: 2015
The transition of Rhodes University graduates into the South African labour market : a case study of the 2010 cohort
- Authors: Ntikinca, Kanyiso Lungani
- Date: 2015
- Subjects: Rhodes University -- Graduate students , Labor market -- South Africa , College graduates -- Employment -- South Africa , Labor supply -- Research -- South Africa , Market segmentation -- South Africa , Employability -- South Africa , Race discrimination -- South Africa , Sex discrimination -- South Africa
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3395 , http://hdl.handle.net/10962/d1018198
- Description: Recent studies have shown that graduates from historically White universities (HWUs) experience better labour market outcomes than graduates from historically Black universities (HBUs). This is a result of the legacy of apartheid which promoted racial inequality in all spheres of South African society, more especially in higher education and the labour market. Post-1994, government dedicated large amounts for the restructuring of the higher education sector of South Africa in order to level out the playing field. However, graduates from HWUs still experience better labour market success than graduates from HBUs. That said, there is limited information about the labour market outcomes and experiences of graduates from a former White university (especially graduates from Rhodes University). Therefore, the central aim of this dissertation is to show that graduates from a historically White university (Rhodes University) experience varying and unequal outcomes in the South African labour market on account of (among other factors) their chosen fields of study, race and sex. This study is informed by the heterodox labour market approach, which is partly inspired by the critical realist account of the labour market. As a result, this theoretical framework allowed the researcher to use the Labour Market Segmentation (LMS) theory as a tool to inform this analysis. The study has adopted a quantitative survey design and has incorporated some of the key methodological lessons learned from the collection of international graduate tracer studies. The findings from this study indicated that ‘field of study’ is a strong determiner of the outcomes of Rhodes graduates in the labour market. This was visible in the persistence of a skills bias towards commerce and science graduates. Evidently, even when we controlled for race and sex, graduates from the commerce and science faculties experience better labour market outcomes than humanities graduates. This is a result of a skills biased South African economy, which has a higher demand for certain skills over others. However, the findings from this study also show evidence of pre-labour market discrimination and inequality (based on race and sex) in the supply-side institutions such as the family, schooling and university. The findings also show continuities and discontinuities of labour market discrimination (based on race and sex) in the outcomes of Rhodes graduates in the South African labour market. More importantly, this dissertation indicates that Rhodes graduates experience varying outcomes in the labour market as a result of (among other factors) their chosen fields of study, race and sex.
- Full Text:
- Date Issued: 2015
- Authors: Ntikinca, Kanyiso Lungani
- Date: 2015
- Subjects: Rhodes University -- Graduate students , Labor market -- South Africa , College graduates -- Employment -- South Africa , Labor supply -- Research -- South Africa , Market segmentation -- South Africa , Employability -- South Africa , Race discrimination -- South Africa , Sex discrimination -- South Africa
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3395 , http://hdl.handle.net/10962/d1018198
- Description: Recent studies have shown that graduates from historically White universities (HWUs) experience better labour market outcomes than graduates from historically Black universities (HBUs). This is a result of the legacy of apartheid which promoted racial inequality in all spheres of South African society, more especially in higher education and the labour market. Post-1994, government dedicated large amounts for the restructuring of the higher education sector of South Africa in order to level out the playing field. However, graduates from HWUs still experience better labour market success than graduates from HBUs. That said, there is limited information about the labour market outcomes and experiences of graduates from a former White university (especially graduates from Rhodes University). Therefore, the central aim of this dissertation is to show that graduates from a historically White university (Rhodes University) experience varying and unequal outcomes in the South African labour market on account of (among other factors) their chosen fields of study, race and sex. This study is informed by the heterodox labour market approach, which is partly inspired by the critical realist account of the labour market. As a result, this theoretical framework allowed the researcher to use the Labour Market Segmentation (LMS) theory as a tool to inform this analysis. The study has adopted a quantitative survey design and has incorporated some of the key methodological lessons learned from the collection of international graduate tracer studies. The findings from this study indicated that ‘field of study’ is a strong determiner of the outcomes of Rhodes graduates in the labour market. This was visible in the persistence of a skills bias towards commerce and science graduates. Evidently, even when we controlled for race and sex, graduates from the commerce and science faculties experience better labour market outcomes than humanities graduates. This is a result of a skills biased South African economy, which has a higher demand for certain skills over others. However, the findings from this study also show evidence of pre-labour market discrimination and inequality (based on race and sex) in the supply-side institutions such as the family, schooling and university. The findings also show continuities and discontinuities of labour market discrimination (based on race and sex) in the outcomes of Rhodes graduates in the South African labour market. More importantly, this dissertation indicates that Rhodes graduates experience varying outcomes in the labour market as a result of (among other factors) their chosen fields of study, race and sex.
- Full Text:
- Date Issued: 2015
The effect of extracellular Hsp90β and TGF-β1 on colon cancer biology
- Authors: Perks, Tamarin
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/55896 , vital:26753
- Description: The TGF-β signaling pathway is known to be one of the most commonly mutated pathways in human cancers, while Hsp90 is a bone fide drug target that is involved in regulating the conformation and activity of many oncoproteins. The role of intracellular Hsp90 in cancer has thus far been established and there is a growing link between extracellular Hsp90 and cancer metastasis, as well as the role of TGF-β in metastasis. This study aimed to analyse the interaction between Hsp90 (both intracellular and extracellular) and the TGF-β machinery in cancer cells, as well as to determine the effect of these proteins on cellular responses on the biology of cancer cells. This was achieved by studying the expression of Hsp90; TGF-βRII and TGF-β1 in cancer cell lines of various origins using flow cytometry, ELISA, and western blot analysis. The genetically paired SW480 and SW620 colon cancer cell lines, derived from a primary tumour and lymph node metastasis, respectively, were selected for further study due to differences in expression levels and activation of the TGF-β1 pathway. SW480 cells expressed double the level of TGF-βRII compared to SW620 cells, while SW620 expressed two times more extracellular TGF-β1 than SW480 cells. A direct interaction between TGF-β1 and Hsp90β was determined in vitro, and confirmed in vivo in SW620 cells. Growth, adhesion and migration were analysed in SW480 and SW620 cells. SW480 cells adhered significantly faster than SW620 cells, while SW620 cells had a greater rate of migration. Inhibiting the TGF-β pathway, specifically TGF-βRI, using SB 431542, as well as inhibiting Hsp90 with novobiocin, caused an increase in migration in SW480 cells. Only the addition of TGF-β1 in combination with Hsp90 as well as SB 431542 caused an increase in migration in SW620 cells. The canonical TGF-β1/TGF-βRI/TGF-βRII pathway may be constitutively active in SW620 cells and the inhibition of TGF-βRI may suggest an alternate pathway or receptor in both SW480 and SW620 cells.
- Full Text:
- Date Issued: 2015
- Authors: Perks, Tamarin
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/55896 , vital:26753
- Description: The TGF-β signaling pathway is known to be one of the most commonly mutated pathways in human cancers, while Hsp90 is a bone fide drug target that is involved in regulating the conformation and activity of many oncoproteins. The role of intracellular Hsp90 in cancer has thus far been established and there is a growing link between extracellular Hsp90 and cancer metastasis, as well as the role of TGF-β in metastasis. This study aimed to analyse the interaction between Hsp90 (both intracellular and extracellular) and the TGF-β machinery in cancer cells, as well as to determine the effect of these proteins on cellular responses on the biology of cancer cells. This was achieved by studying the expression of Hsp90; TGF-βRII and TGF-β1 in cancer cell lines of various origins using flow cytometry, ELISA, and western blot analysis. The genetically paired SW480 and SW620 colon cancer cell lines, derived from a primary tumour and lymph node metastasis, respectively, were selected for further study due to differences in expression levels and activation of the TGF-β1 pathway. SW480 cells expressed double the level of TGF-βRII compared to SW620 cells, while SW620 expressed two times more extracellular TGF-β1 than SW480 cells. A direct interaction between TGF-β1 and Hsp90β was determined in vitro, and confirmed in vivo in SW620 cells. Growth, adhesion and migration were analysed in SW480 and SW620 cells. SW480 cells adhered significantly faster than SW620 cells, while SW620 cells had a greater rate of migration. Inhibiting the TGF-β pathway, specifically TGF-βRI, using SB 431542, as well as inhibiting Hsp90 with novobiocin, caused an increase in migration in SW480 cells. Only the addition of TGF-β1 in combination with Hsp90 as well as SB 431542 caused an increase in migration in SW620 cells. The canonical TGF-β1/TGF-βRI/TGF-βRII pathway may be constitutively active in SW620 cells and the inhibition of TGF-βRI may suggest an alternate pathway or receptor in both SW480 and SW620 cells.
- Full Text:
- Date Issued: 2015
Perceived breach and violation of the psychological contract in a collectivistic culture
- Van der Merwe, Sophie Wilhelmine
- Authors: Van der Merwe, Sophie Wilhelmine
- Date: 2015
- Subjects: Collectivism , Employees -- Attitudes , Contracts -- Psychological aspects , Industrial relations , Breach of contract , Personnel management -- Psychological aspects , Organizational behavior , Psychology, Industrial
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:855 , http://hdl.handle.net/10962/d1017523
- Description: The significance of relationships on economic actions and employee behaviour makes it critical for employers to understand the dynamics of employment through mutual obligations (Rousseau, 1990; Guest, 2004b). The psychological contract affords a broad platform to study the employment relationship (Thomas et al., 2010), and is an important tool for organisational success (McDermott et al., 2013). The literature review contained in this study indicates the differences in contracting environments due to the prevailing cultural orientation. However, there is a dearth of research in collectivistic culture, to which this study will add. The literature also makes a distinction between perceptions of breach and violation. While perceptions of breach of the psychological contract is the perception that the employer has not met all obligations and promises, violation is the emotional and affective state following breach (Morrison & Robinson, 1997) and results in negative or deviant behaviours (Chiu & Peng, 2008). Both breach and violation perceptions negatively affect employee behaviours and attitudes in the workplace (Aggarwal & Bhargava, 2014). This research assumed a constructivist paradigm and builds understanding of the outcomes of breach and violation of the psychological contract on employees’ working life in a collectivistic environment. Primary data collection was by in-depth semi-structured, one on one interviews with five employees of a state-subsidised organisation in East London, making use of convenience sampling. Follow up interviews were conducted, resulting in 7ₑ/₄ hours of interviewing time. Cultural orientation was ascertained through the use of a questionnaire. The findings of this study confirmed that the type of psychological contract entered into influences the outcomes of perceptions of breach and violation. Both dimensions of collectivism, namely institutional and in-group, were practised in this environment, which also impacted on both the individual’s experience and outcomes for the organisation. The latter was influenced by commitment to organisational goals and supervisory or collegial relationships. Most notable of the results is the effect of expectations of transitional justice on experience of the psychological contract. Practical implications and recommendations for future research are made. This research is presented in three sections; firstly the research is presented in the format of an academic paper and includes a concise summary of literature and research method. The second section is an expanded literature review of the psychological contract and its influencing factors, as well as the outcomes of breach and violation. The last section describes and justifies in detail the design of the research and the research procedure followed. , Alternate name: Van der Merwe, Somine
- Full Text:
- Date Issued: 2015
- Authors: Van der Merwe, Sophie Wilhelmine
- Date: 2015
- Subjects: Collectivism , Employees -- Attitudes , Contracts -- Psychological aspects , Industrial relations , Breach of contract , Personnel management -- Psychological aspects , Organizational behavior , Psychology, Industrial
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:855 , http://hdl.handle.net/10962/d1017523
- Description: The significance of relationships on economic actions and employee behaviour makes it critical for employers to understand the dynamics of employment through mutual obligations (Rousseau, 1990; Guest, 2004b). The psychological contract affords a broad platform to study the employment relationship (Thomas et al., 2010), and is an important tool for organisational success (McDermott et al., 2013). The literature review contained in this study indicates the differences in contracting environments due to the prevailing cultural orientation. However, there is a dearth of research in collectivistic culture, to which this study will add. The literature also makes a distinction between perceptions of breach and violation. While perceptions of breach of the psychological contract is the perception that the employer has not met all obligations and promises, violation is the emotional and affective state following breach (Morrison & Robinson, 1997) and results in negative or deviant behaviours (Chiu & Peng, 2008). Both breach and violation perceptions negatively affect employee behaviours and attitudes in the workplace (Aggarwal & Bhargava, 2014). This research assumed a constructivist paradigm and builds understanding of the outcomes of breach and violation of the psychological contract on employees’ working life in a collectivistic environment. Primary data collection was by in-depth semi-structured, one on one interviews with five employees of a state-subsidised organisation in East London, making use of convenience sampling. Follow up interviews were conducted, resulting in 7ₑ/₄ hours of interviewing time. Cultural orientation was ascertained through the use of a questionnaire. The findings of this study confirmed that the type of psychological contract entered into influences the outcomes of perceptions of breach and violation. Both dimensions of collectivism, namely institutional and in-group, were practised in this environment, which also impacted on both the individual’s experience and outcomes for the organisation. The latter was influenced by commitment to organisational goals and supervisory or collegial relationships. Most notable of the results is the effect of expectations of transitional justice on experience of the psychological contract. Practical implications and recommendations for future research are made. This research is presented in three sections; firstly the research is presented in the format of an academic paper and includes a concise summary of literature and research method. The second section is an expanded literature review of the psychological contract and its influencing factors, as well as the outcomes of breach and violation. The last section describes and justifies in detail the design of the research and the research procedure followed. , Alternate name: Van der Merwe, Somine
- Full Text:
- Date Issued: 2015
A sociological analysis of the production, marketing and distribution of contemporary popular music by Zambian musicians
- Authors: Kazadi, Kanyabu Solomon
- Date: 2015
- Subjects: Popular music -- Zambia , Musicians -- Zambia , Sound recording industry -- Zambia , Popular music -- Production and direction -- Zambia , Popular music -- Marketing , Intellectual property -- Zambia , Copyright -- Royalties -- Zambia
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3404 , http://hdl.handle.net/10962/d1018933
- Description: The purpose of this research was to gather information about the production, marketing and distribution of Zambian contemporary music by Zambian musicians. Very little information has been documented about the development of the Zambian music industry, particularly from the perspective of those within the industry. As a result this study attempted to add to this knowledge. To achieve this Pierre Bourdieu’s theoretical concepts of ‘fields’ and ‘habitus’ were used to gain an understanding of what affects the creation of art forms such as music as well as the structures and underlying processes within the music industry. The concept of ‘fields’ usefully framed an explanation of the struggles and connections within the various fields in the industry and a view of the Zambian music industry in relation to the international industry. To gather the data necessary for this research a qualitative approach was utilised involving semistructured in-depth questionnaires from twenty-three interviewees. These interviewees were selected from various sectors of the music industry in an attempt to gain a holistic perspective of the industry in the 21st century. There were four subgroups: the artists (singers, rappers and instrumentalists), managers, radio DJs, and a miscellaneous group made up of the remaining participants, a Sounds Arcade manager, a music journalist, the National Arts Council Chairperson, a Zambia Music Copyright Protection Society (ZAMCOPS) administrator, and the then President of the Zambia Association of Musicians (ZAM). With the limited exposure to formal musical, instrumental and production training, musicians, instrumentalists, managers and studio production personnel interviewed had had to learn their craft on-the-job. This limited knowledge appears to add to the hindrance of the development of careers and the industry, particularly in terms of how to register and distribute music correctly to earn royalties and protect their intellectual property against piracy. From an institutional level piracy is being addressed more forcefully with the introduction of holograms and the tightening of policies and structures to do with the music industry.
- Full Text:
- Date Issued: 2015
- Authors: Kazadi, Kanyabu Solomon
- Date: 2015
- Subjects: Popular music -- Zambia , Musicians -- Zambia , Sound recording industry -- Zambia , Popular music -- Production and direction -- Zambia , Popular music -- Marketing , Intellectual property -- Zambia , Copyright -- Royalties -- Zambia
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3404 , http://hdl.handle.net/10962/d1018933
- Description: The purpose of this research was to gather information about the production, marketing and distribution of Zambian contemporary music by Zambian musicians. Very little information has been documented about the development of the Zambian music industry, particularly from the perspective of those within the industry. As a result this study attempted to add to this knowledge. To achieve this Pierre Bourdieu’s theoretical concepts of ‘fields’ and ‘habitus’ were used to gain an understanding of what affects the creation of art forms such as music as well as the structures and underlying processes within the music industry. The concept of ‘fields’ usefully framed an explanation of the struggles and connections within the various fields in the industry and a view of the Zambian music industry in relation to the international industry. To gather the data necessary for this research a qualitative approach was utilised involving semistructured in-depth questionnaires from twenty-three interviewees. These interviewees were selected from various sectors of the music industry in an attempt to gain a holistic perspective of the industry in the 21st century. There were four subgroups: the artists (singers, rappers and instrumentalists), managers, radio DJs, and a miscellaneous group made up of the remaining participants, a Sounds Arcade manager, a music journalist, the National Arts Council Chairperson, a Zambia Music Copyright Protection Society (ZAMCOPS) administrator, and the then President of the Zambia Association of Musicians (ZAM). With the limited exposure to formal musical, instrumental and production training, musicians, instrumentalists, managers and studio production personnel interviewed had had to learn their craft on-the-job. This limited knowledge appears to add to the hindrance of the development of careers and the industry, particularly in terms of how to register and distribute music correctly to earn royalties and protect their intellectual property against piracy. From an institutional level piracy is being addressed more forcefully with the introduction of holograms and the tightening of policies and structures to do with the music industry.
- Full Text:
- Date Issued: 2015
Language policy and practice in Eastern Cape courtrooms with reference to interpretation in selected cases
- Authors: Mpahlwa, Matthew Xola
- Date: 2015
- Subjects: Translating and interpreting , Translating and interpreting -- Errors , Court interpreting and translating -- South Africa -- Eastern Cape , Translators -- Training of -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3656 , http://hdl.handle.net/10962/d1018658
- Description: This study seeks to find what problems and process of interpreting are experienced by professional interpreters in the criminal justice system in South Africa. This study commences with an outlook of the origins and development of types of interpretation and then proceeds with critical review of scholarly literature dealing with interpretation in multilingual courtroom. This study explores the flawed language policy and its impracticality for the Eastern Cape courtrooms. This study undertakes a critical analysis of the current legislation (Bills & Acts).This study explores the extent to which the court automatic review proceedings act as a gatekeeper in ensuring against prejudice that can result in the non-use and use of indigenous languages in the trial courtroom within the Eastern Cape jurisdiction. Furthermore this study focuses on cases taken for review based on mis-understanding, mis-communication and wrongful interpretation that result in irregularities that appear on court records. This study also investigates the primary barriers for the use of African languages as languages of record in the courtroom. An eclectic sociolinguistic approach which encompasses the ethnography of speaking, and discourse analysis (observation in the courtroom) is used as a methodology in this study. Furthermore, the analysis of case-law forms part of the methodology alongside court observation.This study saw court actors from different spheres of the legal profession give their personal views and encounters with regards the art and the state of court interpreting in the province of the Eastern Cape.This state of affairs may have disastrous and far-reaching effects in that incorrect and/or imperfect translation may relate to the very facts that are crucial for the determination of the case. At the end recommendations are given on how to remedy the current state of affairs.
- Full Text:
- Date Issued: 2015
- Authors: Mpahlwa, Matthew Xola
- Date: 2015
- Subjects: Translating and interpreting , Translating and interpreting -- Errors , Court interpreting and translating -- South Africa -- Eastern Cape , Translators -- Training of -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3656 , http://hdl.handle.net/10962/d1018658
- Description: This study seeks to find what problems and process of interpreting are experienced by professional interpreters in the criminal justice system in South Africa. This study commences with an outlook of the origins and development of types of interpretation and then proceeds with critical review of scholarly literature dealing with interpretation in multilingual courtroom. This study explores the flawed language policy and its impracticality for the Eastern Cape courtrooms. This study undertakes a critical analysis of the current legislation (Bills & Acts).This study explores the extent to which the court automatic review proceedings act as a gatekeeper in ensuring against prejudice that can result in the non-use and use of indigenous languages in the trial courtroom within the Eastern Cape jurisdiction. Furthermore this study focuses on cases taken for review based on mis-understanding, mis-communication and wrongful interpretation that result in irregularities that appear on court records. This study also investigates the primary barriers for the use of African languages as languages of record in the courtroom. An eclectic sociolinguistic approach which encompasses the ethnography of speaking, and discourse analysis (observation in the courtroom) is used as a methodology in this study. Furthermore, the analysis of case-law forms part of the methodology alongside court observation.This study saw court actors from different spheres of the legal profession give their personal views and encounters with regards the art and the state of court interpreting in the province of the Eastern Cape.This state of affairs may have disastrous and far-reaching effects in that incorrect and/or imperfect translation may relate to the very facts that are crucial for the determination of the case. At the end recommendations are given on how to remedy the current state of affairs.
- Full Text:
- Date Issued: 2015
An investigation into the feasibility of incorporating ketoconazole into solid lipid microparticles
- Authors: Jhundoo, Henusha Devi
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10962/54701 , vital:26601
- Description: One of the major challenges of the oral administration of ketoconazole (KTZ), an inhibitor of sterol 14α demethylase, used in the management of systemic and topical mycoses in immuno-compromised and paediatric patients is the lack of availability of liquid dosage forms. In order to overcome this challenge, extemporaneous preparations have been manufactured by care-givers and health care providers by crushing or breaking solid oral dosage forms of KTZ and mixing with a vehicle to produce a liquid dosage form that can be swallowed by patients. However, the use of extemporaneous preparations may lead to under or over-dosing if the care-givers are not guided accordingly. Furthermore, the dearth of information on the stability of these KTZ-containing extemporaneous preparations may lead to ineffective antifungal therapy and complicate the problems of resistance as it is difficult to estimate the shelf-lives of these extemporaneous products under varying storage conditions due to the susceptibility of KTZ to chemical degradation. Therefore, there is a need for formulation scientists to develop novel drug delivery systems that avoid the need for extemporaneous preparations, possess well-established limits of stability and minimize the risks of systemic adverse effects to facilitate KTZ therapy. The use of solid lipid microparticles (SLM) as potential carriers for the oral administration of KTZ was investigated since solid lipid carriers are known to exhibit the advantages of traditional colloidal carriers. The research undertaken in these studies aimed to investigate the feasibility of developing and manufacturing solid lipid microparticles (SLM), using a simple micro-emulsion technique, as a carrier for KTZ. Prior to pre-formulation, formulation development and optimization studies of KTZ-loaded SLM, it was necessary to develop and validate an analytical method for the in vitro quantitation and characterization of KTZ in aqueous dispersions of SLM during development and assessment studies. An accurate, precise, specific and sensitive reversed-phase high performance liquid chromatographic (RP-HPLC) method coupled with UV detection at 206 nm was developed, optimized and validated for the analysis of KTZ in formulations. Formulation development studies were preceded by solubility studies of KTZ in different lipids. Labrafil® M2130 CS was found to exhibit the best solubilising potential for KTZ. Pre-formulation studies were also designed to determine the polymorphic behavior and the crystallinity of KTZ and Labrafil® M2130 CS that was used for subsequent manufacture of the solid lipid carriers. DSC and FTIR studies revealed that there were no changes in the crystallinity of KTZ or Labrafil® M2130 CS following exposure to a temperature of 60°C for 1 hour. In addition the potential for physicochemical interaction of KTZ with the lipid Labrafil® M2130 CS was investigated using DSC and FTIR and the results revealed that KTZ was molecularly dispersed in Labrafil® M2130 CS and that it is unlikely that KTZ would interact with the lipid. It was therefore established that KTZ and Labrafil® M2130 CS were thermo-stable at a temperature of 60°C and thus a micro-emulsion technique could be used to manufacture the KTZ-loaded SLM. Drug-free and KTZ-loaded SLM were prepared using a modified micro-emulsion technique that required the use of an Ultra-Turrax® homogenizer set at 24 000 rpm for 5 minutes followed by the use of the Erweka GmbH homogenizer. SLM were characterized in terms of particle size (PS), zeta potential (ZP), shape and surface morphology using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In addition drug loading capacity (DLC) and encapsulation efficiency (EE) of SLM for KTZ were assessed using RP-HPLC. Formulation development and optimization studies of KTZ-loaded SLM were initially aimed at selecting an emulsifying system that was able to stabilize the SLM in an aqueous dispersion. Successful formulations were selected based on their ability to remain physically stable on the day of manufacture. Pluronic® F68 used in combination with Lutrol® E40, Soluphor® P, Soluplus® produced unstable dispersions on the day of manufacture and these combinations were not investigated further. However, the formulation of a stable KTZ-loaded SLM dispersion was accomplished by use of a combination of Pluronic® F68, Tween 80 and sodium cholate as the surfactant system. Increasing amounts of Labrafil® M2130 CS resulted in the production of particles with low DLC and EE, a large PS and a relatively unchanged ZP. An optimum concentration of 10% w/v Labrafil® M2130 CS was selected to manufacture the KTZ-loaded SLM. Studies to determine the influence of KTZ loading on the quality of SLM revealed that concentrations of KTZ > 5% w/v led to a reduction in DLC and EE and an increase in PS with minimal impact on the ZP. Stability studies conducted at 25°C/65% RH and 40°C/75% RH for up to 30 days following manufacture revealed that batch SLM 15 manufactured using 10% w/v Labrafil® M2130 CS, 5% w/v KTZ and a combination of 4% w/v Pluronic® F-68, 2% w/v Tween 80 and 1% w/v sodium cholate produced the most stable dosage form when stored at 25°C/65% RH for up to 30 days. However, storage at 40°C/75% RH resulted in instability of the formulation. An aqueous dispersion of KTZ-loaded SLM has been developed and assessed and may offer an alternative to extemporaneous preparations used for KTZ therapy in paediatric and immuno-compromised patients.
- Full Text:
- Date Issued: 2015
- Authors: Jhundoo, Henusha Devi
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10962/54701 , vital:26601
- Description: One of the major challenges of the oral administration of ketoconazole (KTZ), an inhibitor of sterol 14α demethylase, used in the management of systemic and topical mycoses in immuno-compromised and paediatric patients is the lack of availability of liquid dosage forms. In order to overcome this challenge, extemporaneous preparations have been manufactured by care-givers and health care providers by crushing or breaking solid oral dosage forms of KTZ and mixing with a vehicle to produce a liquid dosage form that can be swallowed by patients. However, the use of extemporaneous preparations may lead to under or over-dosing if the care-givers are not guided accordingly. Furthermore, the dearth of information on the stability of these KTZ-containing extemporaneous preparations may lead to ineffective antifungal therapy and complicate the problems of resistance as it is difficult to estimate the shelf-lives of these extemporaneous products under varying storage conditions due to the susceptibility of KTZ to chemical degradation. Therefore, there is a need for formulation scientists to develop novel drug delivery systems that avoid the need for extemporaneous preparations, possess well-established limits of stability and minimize the risks of systemic adverse effects to facilitate KTZ therapy. The use of solid lipid microparticles (SLM) as potential carriers for the oral administration of KTZ was investigated since solid lipid carriers are known to exhibit the advantages of traditional colloidal carriers. The research undertaken in these studies aimed to investigate the feasibility of developing and manufacturing solid lipid microparticles (SLM), using a simple micro-emulsion technique, as a carrier for KTZ. Prior to pre-formulation, formulation development and optimization studies of KTZ-loaded SLM, it was necessary to develop and validate an analytical method for the in vitro quantitation and characterization of KTZ in aqueous dispersions of SLM during development and assessment studies. An accurate, precise, specific and sensitive reversed-phase high performance liquid chromatographic (RP-HPLC) method coupled with UV detection at 206 nm was developed, optimized and validated for the analysis of KTZ in formulations. Formulation development studies were preceded by solubility studies of KTZ in different lipids. Labrafil® M2130 CS was found to exhibit the best solubilising potential for KTZ. Pre-formulation studies were also designed to determine the polymorphic behavior and the crystallinity of KTZ and Labrafil® M2130 CS that was used for subsequent manufacture of the solid lipid carriers. DSC and FTIR studies revealed that there were no changes in the crystallinity of KTZ or Labrafil® M2130 CS following exposure to a temperature of 60°C for 1 hour. In addition the potential for physicochemical interaction of KTZ with the lipid Labrafil® M2130 CS was investigated using DSC and FTIR and the results revealed that KTZ was molecularly dispersed in Labrafil® M2130 CS and that it is unlikely that KTZ would interact with the lipid. It was therefore established that KTZ and Labrafil® M2130 CS were thermo-stable at a temperature of 60°C and thus a micro-emulsion technique could be used to manufacture the KTZ-loaded SLM. Drug-free and KTZ-loaded SLM were prepared using a modified micro-emulsion technique that required the use of an Ultra-Turrax® homogenizer set at 24 000 rpm for 5 minutes followed by the use of the Erweka GmbH homogenizer. SLM were characterized in terms of particle size (PS), zeta potential (ZP), shape and surface morphology using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In addition drug loading capacity (DLC) and encapsulation efficiency (EE) of SLM for KTZ were assessed using RP-HPLC. Formulation development and optimization studies of KTZ-loaded SLM were initially aimed at selecting an emulsifying system that was able to stabilize the SLM in an aqueous dispersion. Successful formulations were selected based on their ability to remain physically stable on the day of manufacture. Pluronic® F68 used in combination with Lutrol® E40, Soluphor® P, Soluplus® produced unstable dispersions on the day of manufacture and these combinations were not investigated further. However, the formulation of a stable KTZ-loaded SLM dispersion was accomplished by use of a combination of Pluronic® F68, Tween 80 and sodium cholate as the surfactant system. Increasing amounts of Labrafil® M2130 CS resulted in the production of particles with low DLC and EE, a large PS and a relatively unchanged ZP. An optimum concentration of 10% w/v Labrafil® M2130 CS was selected to manufacture the KTZ-loaded SLM. Studies to determine the influence of KTZ loading on the quality of SLM revealed that concentrations of KTZ > 5% w/v led to a reduction in DLC and EE and an increase in PS with minimal impact on the ZP. Stability studies conducted at 25°C/65% RH and 40°C/75% RH for up to 30 days following manufacture revealed that batch SLM 15 manufactured using 10% w/v Labrafil® M2130 CS, 5% w/v KTZ and a combination of 4% w/v Pluronic® F-68, 2% w/v Tween 80 and 1% w/v sodium cholate produced the most stable dosage form when stored at 25°C/65% RH for up to 30 days. However, storage at 40°C/75% RH resulted in instability of the formulation. An aqueous dispersion of KTZ-loaded SLM has been developed and assessed and may offer an alternative to extemporaneous preparations used for KTZ therapy in paediatric and immuno-compromised patients.
- Full Text:
- Date Issued: 2015
Public understanding of malaria in pregnancy : selected Dar es Salaam audiences' reception of the health education film Chumo
- Authors: Nkwera, Godfrey
- Date: 2015
- Subjects: Chumo (Television program) , Malaria in pregnancy -- Tanzania -- Dar es Salaam , Health education -- Tanzania -- Dar es Salaam , Communication in medicine -- Tanzania -- Dar es Salaam , Sex role -- Tanzania -- Dar es Salaam , Health behavior -- Tanzania -- Dar es Salaam , Health behavior -- Sex differences -- Tanzania -- Dar es Salaam
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3544 , http://hdl.handle.net/10962/d1017785
- Description: This study examines the impact of a health education film, Chumo, in Dar es Salaam on knowledge about malaria in pregnancy. Specifically, the study examines the meanings that the selected audiences make after watching the film. Drawing on the tradition of ‘reception studies’, the data for this study was generated through focus group discussions. These discussions were preceded by thematic analysis of the film and its script. An analysis of the audiences’ responses reveals that Chumo, mostly, successfully conveyed new knowledge about malaria in pregnancy, and reinforced existing knowledge bases about the disease. The audiences were able to ‘decipher’ most of the preferred meanings (of the producers) with regard to the disease, particularly in relation to the transmission of the disease and its prevention in pregnant woman. For example, the analysis indicates that both women and men become more aware of the importance of attending antenatal care sessions at local clinics (hereafter ANC). An interesting finding is that men, mostly, expressed a reluctance to attend ANC with their wives because they fear having to undergo HIV/AIDS testing. Men also expressed the sentiment that attending ANC is a women’s responsibility. The discussion groups also raised issues about the use of insecticide-treated nets - some people believe that using them will affect their health because of the chemicals used to treat the nets. From the reception analysis, various other meanings and themes, relating to the choice of storylines and gender stereotypes used in the story, were raised in discussion. The study attempted to assess whether the storyline was advantageous in conveying the core educational messages, or if some elements of the storyline either ‘got in the way’ or reinforced gender roles in ways that may or may not be helpful in terms of combatting malaria in pregnancy. The study also found that Chumo stimulated interpersonal communication, which may trigger behaviour change. It can be demonstrated, at least for the participants in these focus groups, that the film motivated positive attitudes towards behaviour change, i.e. created at least some intention to change. However, interpersonal communication and attitude to change are not, of course, actual change of behaviour: these elements only indicate the possibility of behaviour change in the future. Further study needs to be undertaken to explore whether the actual change took place and whether the change is a result of the exposure to Chumo.
- Full Text:
- Date Issued: 2015
- Authors: Nkwera, Godfrey
- Date: 2015
- Subjects: Chumo (Television program) , Malaria in pregnancy -- Tanzania -- Dar es Salaam , Health education -- Tanzania -- Dar es Salaam , Communication in medicine -- Tanzania -- Dar es Salaam , Sex role -- Tanzania -- Dar es Salaam , Health behavior -- Tanzania -- Dar es Salaam , Health behavior -- Sex differences -- Tanzania -- Dar es Salaam
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3544 , http://hdl.handle.net/10962/d1017785
- Description: This study examines the impact of a health education film, Chumo, in Dar es Salaam on knowledge about malaria in pregnancy. Specifically, the study examines the meanings that the selected audiences make after watching the film. Drawing on the tradition of ‘reception studies’, the data for this study was generated through focus group discussions. These discussions were preceded by thematic analysis of the film and its script. An analysis of the audiences’ responses reveals that Chumo, mostly, successfully conveyed new knowledge about malaria in pregnancy, and reinforced existing knowledge bases about the disease. The audiences were able to ‘decipher’ most of the preferred meanings (of the producers) with regard to the disease, particularly in relation to the transmission of the disease and its prevention in pregnant woman. For example, the analysis indicates that both women and men become more aware of the importance of attending antenatal care sessions at local clinics (hereafter ANC). An interesting finding is that men, mostly, expressed a reluctance to attend ANC with their wives because they fear having to undergo HIV/AIDS testing. Men also expressed the sentiment that attending ANC is a women’s responsibility. The discussion groups also raised issues about the use of insecticide-treated nets - some people believe that using them will affect their health because of the chemicals used to treat the nets. From the reception analysis, various other meanings and themes, relating to the choice of storylines and gender stereotypes used in the story, were raised in discussion. The study attempted to assess whether the storyline was advantageous in conveying the core educational messages, or if some elements of the storyline either ‘got in the way’ or reinforced gender roles in ways that may or may not be helpful in terms of combatting malaria in pregnancy. The study also found that Chumo stimulated interpersonal communication, which may trigger behaviour change. It can be demonstrated, at least for the participants in these focus groups, that the film motivated positive attitudes towards behaviour change, i.e. created at least some intention to change. However, interpersonal communication and attitude to change are not, of course, actual change of behaviour: these elements only indicate the possibility of behaviour change in the future. Further study needs to be undertaken to explore whether the actual change took place and whether the change is a result of the exposure to Chumo.
- Full Text:
- Date Issued: 2015