An ideological analysis of the construction of masculinity in the South African superhero comic book, Kwezi
- Authors: Reyneke, Brendon George
- Date: 2020
- Subjects: Mkize, Loyiso, 1987- -- Kwezi , Superheroes -- South Africa , Comic books, strips, etc. -- South Africa , Graphic novels -- South Africa , Masculinity in literature , Violence in literature , Superheroes, Black
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/144533 , vital:38354
- Description: In 2014, South African artist and comic book illustrator, Loyiso Mkize created Kwezi, South Africa’s first superhero comic book. His comic features the titular Kwezi as a young, black man living alone on the outskirts of Gold City who discovers he has superpowers. Along with Kwezi, the comic is populated by predominantly black African characters – both good and bad. The creation of Kwezi is an important step in the development of comic books in South Africa as it draws from the cultural and physical landscape of the country and speaks to young black people without them having to look outside of the country for superheroes to identify with. Stuart Hall (Hall, 1997, pp. 272-274) asserts that attempts to reclaim the black subject in popular culture tend to go through two phases. In the first phase blackness is liberated from negative representations and is replaced with more positive depictions. Thereafter though, the black subject is produced inside contemporary “regimes of representation”. In this thesis, I will show how Mkize’s representation of Kwezi follows Stuart Hall’s description of the reclamation of black subjectivity. Using narrative theory, visual social semiotics and Thompson’s modes of operational ideology I will show how in his attempt to represent African blackness positively, Mkize overlooks normative genre representations of masculinity and produces a story of a South African that remains unliberated from patriarchy and hegemonic masculinity. Mkize reproduces many of the hegemonic discourses concerning the masculine body, the power difference between male and female characters and subscribes to the justified, violent actions of the masculine superhero. Typically, in superhero comics there is an erasure of the ordinary man in favour of an excessive and powerful one-dimensional masculine ideal (Brown, 1999, pp. 31-32) At the end of my analysis I will show that Kwezi is constructed in this way as a physically strong and muscular, violent and emotionless, self-made man who is in control and overcomes all obstacles.
- Full Text:
- Date Issued: 2020
- Authors: Reyneke, Brendon George
- Date: 2020
- Subjects: Mkize, Loyiso, 1987- -- Kwezi , Superheroes -- South Africa , Comic books, strips, etc. -- South Africa , Graphic novels -- South Africa , Masculinity in literature , Violence in literature , Superheroes, Black
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/144533 , vital:38354
- Description: In 2014, South African artist and comic book illustrator, Loyiso Mkize created Kwezi, South Africa’s first superhero comic book. His comic features the titular Kwezi as a young, black man living alone on the outskirts of Gold City who discovers he has superpowers. Along with Kwezi, the comic is populated by predominantly black African characters – both good and bad. The creation of Kwezi is an important step in the development of comic books in South Africa as it draws from the cultural and physical landscape of the country and speaks to young black people without them having to look outside of the country for superheroes to identify with. Stuart Hall (Hall, 1997, pp. 272-274) asserts that attempts to reclaim the black subject in popular culture tend to go through two phases. In the first phase blackness is liberated from negative representations and is replaced with more positive depictions. Thereafter though, the black subject is produced inside contemporary “regimes of representation”. In this thesis, I will show how Mkize’s representation of Kwezi follows Stuart Hall’s description of the reclamation of black subjectivity. Using narrative theory, visual social semiotics and Thompson’s modes of operational ideology I will show how in his attempt to represent African blackness positively, Mkize overlooks normative genre representations of masculinity and produces a story of a South African that remains unliberated from patriarchy and hegemonic masculinity. Mkize reproduces many of the hegemonic discourses concerning the masculine body, the power difference between male and female characters and subscribes to the justified, violent actions of the masculine superhero. Typically, in superhero comics there is an erasure of the ordinary man in favour of an excessive and powerful one-dimensional masculine ideal (Brown, 1999, pp. 31-32) At the end of my analysis I will show that Kwezi is constructed in this way as a physically strong and muscular, violent and emotionless, self-made man who is in control and overcomes all obstacles.
- Full Text:
- Date Issued: 2020
An analysis of how geogebra can be used as a visualisation tool by selected teachers to develop conceptual understanding of the properties of geometric shapes in grade 9 learners: a case study in Namibia
- Authors: Mwiikeni, Eramus
- Date: 2017
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/6989 , vital:21207
- Description: According to Rosken & Rolka (2006), learning mathematics through visualisations can be a powerful tool to explore mathematical problems and give meaning to mathematical concepts and relationships between them. “Visualisation can reduce the complexity of mathematical problems when dealing with a multitude of information” (p.458). This case study focused on using GeoGebra as a visualisation tool to teach angle properties in Grade 9 geometry. This study set out to analyse how GeoGebra visualisations can be used by selected teachers to teach for conceptual understanding. The research is based on a constructivist view of learning and is oriented within an interpretive paradigm. The methodology used is a qualitative case study. The study was conducted in one school and involved 3 mathematics teachers who were purposefully selected because they showed willingness to use technology in their teaching. I used classroom observations and interviews to collect the data. The study identified a number of factors from the participants that related to using GeoGebra in teaching for conceptual understanding. These include the effective use of dynamic visuals to build on prior knowledge, using multiple representations through image generation and image transformation to make connections and using visuals to justify mathematics ideas. The results from this study indicated that GeoGebra can indeed be used effectively as a teaching tool to teach for conceptual understanding in mathematics.
- Full Text:
- Date Issued: 2017
- Authors: Mwiikeni, Eramus
- Date: 2017
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/6989 , vital:21207
- Description: According to Rosken & Rolka (2006), learning mathematics through visualisations can be a powerful tool to explore mathematical problems and give meaning to mathematical concepts and relationships between them. “Visualisation can reduce the complexity of mathematical problems when dealing with a multitude of information” (p.458). This case study focused on using GeoGebra as a visualisation tool to teach angle properties in Grade 9 geometry. This study set out to analyse how GeoGebra visualisations can be used by selected teachers to teach for conceptual understanding. The research is based on a constructivist view of learning and is oriented within an interpretive paradigm. The methodology used is a qualitative case study. The study was conducted in one school and involved 3 mathematics teachers who were purposefully selected because they showed willingness to use technology in their teaching. I used classroom observations and interviews to collect the data. The study identified a number of factors from the participants that related to using GeoGebra in teaching for conceptual understanding. These include the effective use of dynamic visuals to build on prior knowledge, using multiple representations through image generation and image transformation to make connections and using visuals to justify mathematics ideas. The results from this study indicated that GeoGebra can indeed be used effectively as a teaching tool to teach for conceptual understanding in mathematics.
- Full Text:
- Date Issued: 2017
Waterloo Bay near Mouth of Fish River
- Subjects: Waterloo Bay (South Africa) -- Pictorial works
- Type: Image
- Identifier: vital:14124 , http://hdl.handle.net/10962/d1018048 , MS 10 542 , CD 55
- Full Text: false
- Subjects: Waterloo Bay (South Africa) -- Pictorial works
- Type: Image
- Identifier: vital:14124 , http://hdl.handle.net/10962/d1018048 , MS 10 542 , CD 55
- Full Text: false
The Bender Gestalt Test: an investigation into problems concerning administration and scoring and its application to low-educated adults
- Authors: Dyall, Kate
- Date: 1996
- Subjects: Bender-Gestalt Test , Psychological tests , Brain-damaged children -- Psychological testing
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2968 , http://hdl.handle.net/10962/d1002477 , Bender-Gestalt Test , Psychological tests , Brain-damaged children -- Psychological testing
- Description: The study investigates .the use of the Bender Gestalt Test (BGT) amongst low-educated adults. Three versions of the BGT are used in this study; the original 'copy' version as well as the 'immediate' and 'delayed'recall versions. This is done so as to expand the ability of the BGT to identify neurological impairment and to differentiate between this and functional impairment. A literature review explores the problems of standardization in the administration, scoring and application of all three versions of the test Suggestions are made to correct the problems identified and a novel system of scoring the recall versions are proposed, which allows for the comparison of results of the three versions of the test and which is based on Lacks's (1984) and Weiss's (1970) systems. Administration procedures were also developed to suit the context of the study. The copy, immediate and delayed versions of the BGT were administered to a group of 184 low-educated adults. Statistical analyses revealed significant education effects for the sample tested with regards to both test scores and performance time. The finding of an education effect for performance time is discussed at length, as some literature regards excessive time as a neurological indicator. An anomaly for the group with no education was found to exist, with the scores of these subjects not Significantly different from those with 4-6 years of education. Possible reasons for this were explored. In addition, the findings of this research revealed a plateau effect with those having less than 6 years of education scoring substantially lower than those with 7 years and more. The scores of adults with 7 and more years of education level out with no significant differences between educational levels. This appears to suggest that education effects rather than the developmental maturity level proposed by Koppitz, are involved. In addition, the scores of low-educated adults on the expanded Bender Gestalt Test were significantly lower than those of children with similar educational levels, in other studies. These findings and possible explanations are discussed. The study concludes by suggesting new research areas and emphasizing the urgent need for separate normative data on the expanded BGT for low-educated adults, and the establishment of appropriate 'cut-off' points.
- Full Text:
- Date Issued: 1996
- Authors: Dyall, Kate
- Date: 1996
- Subjects: Bender-Gestalt Test , Psychological tests , Brain-damaged children -- Psychological testing
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2968 , http://hdl.handle.net/10962/d1002477 , Bender-Gestalt Test , Psychological tests , Brain-damaged children -- Psychological testing
- Description: The study investigates .the use of the Bender Gestalt Test (BGT) amongst low-educated adults. Three versions of the BGT are used in this study; the original 'copy' version as well as the 'immediate' and 'delayed'recall versions. This is done so as to expand the ability of the BGT to identify neurological impairment and to differentiate between this and functional impairment. A literature review explores the problems of standardization in the administration, scoring and application of all three versions of the test Suggestions are made to correct the problems identified and a novel system of scoring the recall versions are proposed, which allows for the comparison of results of the three versions of the test and which is based on Lacks's (1984) and Weiss's (1970) systems. Administration procedures were also developed to suit the context of the study. The copy, immediate and delayed versions of the BGT were administered to a group of 184 low-educated adults. Statistical analyses revealed significant education effects for the sample tested with regards to both test scores and performance time. The finding of an education effect for performance time is discussed at length, as some literature regards excessive time as a neurological indicator. An anomaly for the group with no education was found to exist, with the scores of these subjects not Significantly different from those with 4-6 years of education. Possible reasons for this were explored. In addition, the findings of this research revealed a plateau effect with those having less than 6 years of education scoring substantially lower than those with 7 years and more. The scores of adults with 7 and more years of education level out with no significant differences between educational levels. This appears to suggest that education effects rather than the developmental maturity level proposed by Koppitz, are involved. In addition, the scores of low-educated adults on the expanded Bender Gestalt Test were significantly lower than those of children with similar educational levels, in other studies. These findings and possible explanations are discussed. The study concludes by suggesting new research areas and emphasizing the urgent need for separate normative data on the expanded BGT for low-educated adults, and the establishment of appropriate 'cut-off' points.
- Full Text:
- Date Issued: 1996
Grazing sequence pattern and species selection by cattle in the Dohne sourveld
- Authors: Daines, Thomas
- Date: 1976
- Subjects: Grasses -- Research -- South Africa -- Cape of Good Hope , Grasses -- South Africa -- Cape of Good Hope -- Identification , Grazing -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4257 , http://hdl.handle.net/10962/d1010955
- Description: A detailed investigation into the harvesting of the standing grass crop within camps by cattle showed the following: 1. Cattle have a definite order of preference for the components of the Dohne Sourveld grass sward. The most palatable species in order of preference (based on Corrected Species Importance rating) are Themeda triandra, Tristachya hispida, Andropogon appendiculatus, Alloteropsis semialata and Heteropogon contortus. The least preferred species is Elionurus argenteus. 2. The key species, which is defined as the most abundant and productive palatable species, has been identified as Themeda triandra. 3. In harvesting the grass crop, cattle not only have a set preference for species, but also have a set grazing sequence pattern. This can be divided into three main phases. On entering the camp the animals first select the species of their choice, "creaming" these plants (grazed less than 50%) to obtain the most nutritious feed. In their daily search for food they spread their grazing over an increasingly wider area and over a wider range of species. When they can no longer obtain their daily requirements from this "creaming" operation of the more preferred species they return to the most preferred species , which are then heavily grazed (more than 50%) producing the common pattern of area grazing within a camp. These areas increase in size as the cattle graze down the species of their choice, moving to less preferred species as soon as they can no longer obtain their daily intake requirements from the more preferred species. Finally when they have no alternative they graze the least preferred species. 4. Increasing the stocking density does not alter the grazing sequence pattern or change the order of preference for the component species in the sward. It only increases the rate of utilization of the more preferred species and the various phases in the grazing sequence pattern are reached more rapidly. 5. At higher stocking densities the palatable species are more evenly utilized than at lower stocking densities. 6. Cattle graze a greater variety of species when the sward is young and actively growing. As the maturity of the sward increases the cattle concentrate on the more preferred species and are more selective in their grazing. However, their order of preference remains the same. Species that are highly preferred early in the season retain their ranking when the sward is mature. The only exception to this rule is Alloteropsis semialata. Early in the growing season this species is high on the preference list, but as it matures less is taken until when fully mature, it is rejected by cattle. 7. Elionurus argenteus is the least preferred of all the species in the Dohne Sourveld. Cattle will graze this plant as a last resort when grazing a camp early in the season, but reject the plant when it is fully mature. 8. To retain vigour and production of the Dohne Sourveld results of this investigation support the view that grazing management should be based on the principle of controlled selective grazing. Having shown that the more preferred species are over-utilized when attempting to utilize the least preferred species any grazing management that calls for total utilization of all standing vegetation should be rejected. 9. The results of this investigation supports a multicamp system for the Dohne Sourveld where high stocking densities are employed. This will result in the rapid and even utilization of the more preferred species. Management should be based on short period of stay and long period of absence. Cattle should be moved as soon as the key species have been well utilized, but not overgrazed. Cattle should only be returned to the camp when key species have made adequate regrowth following grazing. 10. A method, that is simple to use, is proposed for determining utilization of the veld and key species at different times of the year using the relationship between percentage of ungrazed plants and utilization of the sward and of Themeda triandra, the key grazing species in the sward. 11. Results show that when rested camps are grazed after May, the number of Themeda triandra inflorescences are drastically reduced. Veld that is specifically rested for the production of Themeda triandra seed should be rested from March of the year preceding expected seeding.
- Full Text:
- Date Issued: 1976
- Authors: Daines, Thomas
- Date: 1976
- Subjects: Grasses -- Research -- South Africa -- Cape of Good Hope , Grasses -- South Africa -- Cape of Good Hope -- Identification , Grazing -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4257 , http://hdl.handle.net/10962/d1010955
- Description: A detailed investigation into the harvesting of the standing grass crop within camps by cattle showed the following: 1. Cattle have a definite order of preference for the components of the Dohne Sourveld grass sward. The most palatable species in order of preference (based on Corrected Species Importance rating) are Themeda triandra, Tristachya hispida, Andropogon appendiculatus, Alloteropsis semialata and Heteropogon contortus. The least preferred species is Elionurus argenteus. 2. The key species, which is defined as the most abundant and productive palatable species, has been identified as Themeda triandra. 3. In harvesting the grass crop, cattle not only have a set preference for species, but also have a set grazing sequence pattern. This can be divided into three main phases. On entering the camp the animals first select the species of their choice, "creaming" these plants (grazed less than 50%) to obtain the most nutritious feed. In their daily search for food they spread their grazing over an increasingly wider area and over a wider range of species. When they can no longer obtain their daily requirements from this "creaming" operation of the more preferred species they return to the most preferred species , which are then heavily grazed (more than 50%) producing the common pattern of area grazing within a camp. These areas increase in size as the cattle graze down the species of their choice, moving to less preferred species as soon as they can no longer obtain their daily intake requirements from the more preferred species. Finally when they have no alternative they graze the least preferred species. 4. Increasing the stocking density does not alter the grazing sequence pattern or change the order of preference for the component species in the sward. It only increases the rate of utilization of the more preferred species and the various phases in the grazing sequence pattern are reached more rapidly. 5. At higher stocking densities the palatable species are more evenly utilized than at lower stocking densities. 6. Cattle graze a greater variety of species when the sward is young and actively growing. As the maturity of the sward increases the cattle concentrate on the more preferred species and are more selective in their grazing. However, their order of preference remains the same. Species that are highly preferred early in the season retain their ranking when the sward is mature. The only exception to this rule is Alloteropsis semialata. Early in the growing season this species is high on the preference list, but as it matures less is taken until when fully mature, it is rejected by cattle. 7. Elionurus argenteus is the least preferred of all the species in the Dohne Sourveld. Cattle will graze this plant as a last resort when grazing a camp early in the season, but reject the plant when it is fully mature. 8. To retain vigour and production of the Dohne Sourveld results of this investigation support the view that grazing management should be based on the principle of controlled selective grazing. Having shown that the more preferred species are over-utilized when attempting to utilize the least preferred species any grazing management that calls for total utilization of all standing vegetation should be rejected. 9. The results of this investigation supports a multicamp system for the Dohne Sourveld where high stocking densities are employed. This will result in the rapid and even utilization of the more preferred species. Management should be based on short period of stay and long period of absence. Cattle should be moved as soon as the key species have been well utilized, but not overgrazed. Cattle should only be returned to the camp when key species have made adequate regrowth following grazing. 10. A method, that is simple to use, is proposed for determining utilization of the veld and key species at different times of the year using the relationship between percentage of ungrazed plants and utilization of the sward and of Themeda triandra, the key grazing species in the sward. 11. Results show that when rested camps are grazed after May, the number of Themeda triandra inflorescences are drastically reduced. Veld that is specifically rested for the production of Themeda triandra seed should be rested from March of the year preceding expected seeding.
- Full Text:
- Date Issued: 1976
Semantiese velde en die onderrig en aanleer van Xhosa-woordeskat
- Authors: Venter, Johannes Andreas
- Date: 1980
- Subjects: Xhosa language -- Semantics , Xhosa language -- Vocabulary , Xhosa language -- Study and teaching
- Language: Afrikaans
- Type: Thesis , Masters , MA
- Identifier: vital:3606 , http://hdl.handle.net/10962/d1003814 , Xhosa language -- Semantics , Xhosa language -- Vocabulary , Xhosa language -- Study and teaching
- Description: Uit Inleiding: Hoe belangrik is die aanleer van woordeskat by die verwerwing van 'n vreemde taal? Indien dit van besondere belang is, kan met reg gevra word: watter beginsels moet in ag geneem word by die opstel van metodes en tegnieke om die aanleer van die woordeskat met optimum effektiwiteit te laat plaasvind? Wat is die teoretiese basis vir sulke metodes en tegnieke? Hierdie is enkele vrae wat in hierdie ondersoek aan die orde sal kom. Die begrip "semantiese velde" of "betekenisvelde" is 'n idee wat in die jongste tyd heelwat aandag geniet by linguiste. Die konsep "betekenisveld" veronderstel twee kenmerke van die leksikon: 1. Dat die betekenisaspek van elke individuele leksikale item, kragtens kontras bestaan en dat hierdie kontras tussen betekeniskomponente van leksikale items in die leksikon teregkom, veral binne 'n betekenisveldsiening. 2. Dat die leksikon kragtens die relasies tussen die leksikale items 'n georganiseerde en gestruktureerde versameling is. In ons ondersoek sal dit dus hoofsaaklik gaan oor: 1. Bestaande metodes en tegnieke om woordeskat te onderrig. 2. Bestaande toerieë oor die gestruktureerdheid van woordeskat. 3. Die moontlikhede wat hierdie teorieë inhou, om die onderrig van woordeskat meer effektief te laat plaasvind.
- Full Text:
- Date Issued: 1980
- Authors: Venter, Johannes Andreas
- Date: 1980
- Subjects: Xhosa language -- Semantics , Xhosa language -- Vocabulary , Xhosa language -- Study and teaching
- Language: Afrikaans
- Type: Thesis , Masters , MA
- Identifier: vital:3606 , http://hdl.handle.net/10962/d1003814 , Xhosa language -- Semantics , Xhosa language -- Vocabulary , Xhosa language -- Study and teaching
- Description: Uit Inleiding: Hoe belangrik is die aanleer van woordeskat by die verwerwing van 'n vreemde taal? Indien dit van besondere belang is, kan met reg gevra word: watter beginsels moet in ag geneem word by die opstel van metodes en tegnieke om die aanleer van die woordeskat met optimum effektiwiteit te laat plaasvind? Wat is die teoretiese basis vir sulke metodes en tegnieke? Hierdie is enkele vrae wat in hierdie ondersoek aan die orde sal kom. Die begrip "semantiese velde" of "betekenisvelde" is 'n idee wat in die jongste tyd heelwat aandag geniet by linguiste. Die konsep "betekenisveld" veronderstel twee kenmerke van die leksikon: 1. Dat die betekenisaspek van elke individuele leksikale item, kragtens kontras bestaan en dat hierdie kontras tussen betekeniskomponente van leksikale items in die leksikon teregkom, veral binne 'n betekenisveldsiening. 2. Dat die leksikon kragtens die relasies tussen die leksikale items 'n georganiseerde en gestruktureerde versameling is. In ons ondersoek sal dit dus hoofsaaklik gaan oor: 1. Bestaande metodes en tegnieke om woordeskat te onderrig. 2. Bestaande toerieë oor die gestruktureerdheid van woordeskat. 3. Die moontlikhede wat hierdie teorieë inhou, om die onderrig van woordeskat meer effektief te laat plaasvind.
- Full Text:
- Date Issued: 1980
A hydrogeological investigation of Grahamstown, assessing both the dynamics and quality of the local groundwater system
- Authors: Smetherham, Kyle Norman
- Date: 2019
- Subjects: Hydrogeology -- South Africa -- Makhanda , Water quality -- South Africa -- Makhanda , Groundwater -- Quality -- South Africa -- Makhanda
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/94444 , vital:31045
- Description: In many parts of South Africa, complete allocation of surface water reservoirs together with current drought conditions has led to serious water shortages and subsequent awareness regarding the importance to save water. Grahamstown is no different, with water problems relating to low supply and high demand being compounded by insufficient treatment capacity and aging infrastructure. Groundwater is an alternative water resource that could potentially act as a supplementary and/or emergency supply to the town, reducing the reliability on surface reservoirs. Groundwater however, is a hidden resource and requires an understanding of various aquifer properties and continuous monitoring and modelling so not to permanently disrupt the natural system but rather achieve sustainable management. Grahamstown is situated towards the northern extent of the Cape Fold Belt (CFB) system, within a synclinal fold structure. The local geology forms two local aquifer systems beneath Grahamstown that directly influence both the dynamics and quality of the groundwater. These underground reservoirs are the Witpoort and Dwyka aquifers and can be described as a semi-confined, fractured, quartzitic sandstone aquifer and an unconfined, fractured, tillite aquifer, respectively. Separating these aquifer systems is a shale aquitard, although due to the fractured nature of the rocks in the region there is most likely some groundwater interaction between them. Evaluation of geological formations together with the monitoring of 31 local boreholes presented a valuable conceptualisation of the local system and allowed for the application of methods to estimate recharge. Recharge estimation is one of the most crucial factors when managing aquifer systems as it can be used to determine what proportion of rainfall contributes to the subsurface reservoir and therefore, the sustainable amount that can be extracted. Various methods have been developed to estimate recharge, however due to the uncertainty surrounding groundwater systems, especially fractured aquifers, it was important to apply multiple methods to validate results. The water-table fluctuation (WTF) and cumulative rainfall departure (CRD) are two methods that were used in the present study to determine recharge. These methods rely on water-table changes in boreholes and specifically how they respond to rainfall events. Along with the WTF and CRD methods, a modelling approach was also used to estimate recharge which focused on the dynamics of a natural groundwater outlet, termed the Fairview Spring. This natural spring system is located just outside the main town of Grahamstown, within the Witpoort aquifer system, and is an important water resource to many residents due to poor supply and quality of municipal water. Monitoring the discharge of this spring allowed for the development of a model which attempts to recreate the discharge conditions observed. Along with groundwater recharge, other processes added to the model include evapotranspiration, storage, interflow spring outflow and groundwater outflow. Several different model simulation scenarios provided valuable insight into the greater groundwater dynamics. In terms of groundwater quality, nine borehole samples and one spring sample were analysed for major ions (Ca, Na, K, Cl, Mg, SO4, HCO3), metals (Cu, Fe, Mn) as well as pH and electrical conductivity. Overall electrical conductivity levels and major ion concentrations were lower in the Witpoort aquifer indicating a better groundwater quality compared to that of the Dwyka aquifer. Of the three metals included in the analysis, Mn proved to be the most significant and the highest concentrations were produced for samples that intersected the shale aquitard unit, suggesting that Mn-containing groundwater is drawn from this geological layer. Development of a supplementary and/or emergency groundwater supply requires careful consideration of the geology, quantity, quality, and recharge in the study site. All these aspects were assessed as well as deliberation into the potential infrastructural costs involved. Through conceptualisation of the system; evidence gathered during basic monitoring; and a simple spring model, the current study aimed to explore certain management strategies and recommend potential options going forward. The hidden nature of the resource together with the heterogeneity of fracture networks creates an inevitable uncertainty surrounding the system. Proper development and management of the aquifer can only be achieved if the system is continually monitored, modelled and utilised sustainably.
- Full Text:
- Date Issued: 2019
- Authors: Smetherham, Kyle Norman
- Date: 2019
- Subjects: Hydrogeology -- South Africa -- Makhanda , Water quality -- South Africa -- Makhanda , Groundwater -- Quality -- South Africa -- Makhanda
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/94444 , vital:31045
- Description: In many parts of South Africa, complete allocation of surface water reservoirs together with current drought conditions has led to serious water shortages and subsequent awareness regarding the importance to save water. Grahamstown is no different, with water problems relating to low supply and high demand being compounded by insufficient treatment capacity and aging infrastructure. Groundwater is an alternative water resource that could potentially act as a supplementary and/or emergency supply to the town, reducing the reliability on surface reservoirs. Groundwater however, is a hidden resource and requires an understanding of various aquifer properties and continuous monitoring and modelling so not to permanently disrupt the natural system but rather achieve sustainable management. Grahamstown is situated towards the northern extent of the Cape Fold Belt (CFB) system, within a synclinal fold structure. The local geology forms two local aquifer systems beneath Grahamstown that directly influence both the dynamics and quality of the groundwater. These underground reservoirs are the Witpoort and Dwyka aquifers and can be described as a semi-confined, fractured, quartzitic sandstone aquifer and an unconfined, fractured, tillite aquifer, respectively. Separating these aquifer systems is a shale aquitard, although due to the fractured nature of the rocks in the region there is most likely some groundwater interaction between them. Evaluation of geological formations together with the monitoring of 31 local boreholes presented a valuable conceptualisation of the local system and allowed for the application of methods to estimate recharge. Recharge estimation is one of the most crucial factors when managing aquifer systems as it can be used to determine what proportion of rainfall contributes to the subsurface reservoir and therefore, the sustainable amount that can be extracted. Various methods have been developed to estimate recharge, however due to the uncertainty surrounding groundwater systems, especially fractured aquifers, it was important to apply multiple methods to validate results. The water-table fluctuation (WTF) and cumulative rainfall departure (CRD) are two methods that were used in the present study to determine recharge. These methods rely on water-table changes in boreholes and specifically how they respond to rainfall events. Along with the WTF and CRD methods, a modelling approach was also used to estimate recharge which focused on the dynamics of a natural groundwater outlet, termed the Fairview Spring. This natural spring system is located just outside the main town of Grahamstown, within the Witpoort aquifer system, and is an important water resource to many residents due to poor supply and quality of municipal water. Monitoring the discharge of this spring allowed for the development of a model which attempts to recreate the discharge conditions observed. Along with groundwater recharge, other processes added to the model include evapotranspiration, storage, interflow spring outflow and groundwater outflow. Several different model simulation scenarios provided valuable insight into the greater groundwater dynamics. In terms of groundwater quality, nine borehole samples and one spring sample were analysed for major ions (Ca, Na, K, Cl, Mg, SO4, HCO3), metals (Cu, Fe, Mn) as well as pH and electrical conductivity. Overall electrical conductivity levels and major ion concentrations were lower in the Witpoort aquifer indicating a better groundwater quality compared to that of the Dwyka aquifer. Of the three metals included in the analysis, Mn proved to be the most significant and the highest concentrations were produced for samples that intersected the shale aquitard unit, suggesting that Mn-containing groundwater is drawn from this geological layer. Development of a supplementary and/or emergency groundwater supply requires careful consideration of the geology, quantity, quality, and recharge in the study site. All these aspects were assessed as well as deliberation into the potential infrastructural costs involved. Through conceptualisation of the system; evidence gathered during basic monitoring; and a simple spring model, the current study aimed to explore certain management strategies and recommend potential options going forward. The hidden nature of the resource together with the heterogeneity of fracture networks creates an inevitable uncertainty surrounding the system. Proper development and management of the aquifer can only be achieved if the system is continually monitored, modelled and utilised sustainably.
- Full Text:
- Date Issued: 2019
Popular politics in the rural Western Cape, South Africa: a case study of Ruiterbos
- Authors: Ghedi Alasow, Jonis
- Date: 2019
- Subjects: Political participation -- South Africa -- Cape Town , South Africa -- Politics and government -- 1994- , Land tenure -- Political aspects -- South Africa , Land use, Rural -- Political aspects -- South Africa
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/96165 , vital:31246
- Description: This thesis argues that the philosophical foundations upon which human beings have been engaged have, across various schools of thought, made the mistake of presuming that some people are more modern than others. This suggestion is refuted throughout this thesis. To do this, intellectual traditions that take the fundamental rationality of all human beings as an indispensable starting point are engaged to argue for the need to acknowledge that everyone in the ‘now’ is indeed, of the ‘now’. This thesis connects these debates about modernity, rationality and humanity to the contemporary discussions around rural politics with particular reference to Ruiterbos in the Western Cape province of South Africa. By means of detailed empirical and ethnographic research, this thesis illustrates the issues around which people in Ruiterbos are politicised. Via this case study, the a priori assumption that rural politics will necessarily manifest itself only with respect to questions of land and agrarian reform or labour relations is complicated. The two issues around which people in Ruiterbos, during the time of this research, seem to be politicised – housing and education – are surfaced throughout this thesis. The thesis argues that the findings in this case study call for an expansion of the issues that are traditionally considered when the question of rural politics is raised. The often historicist approach that limits the possibilities for politics in rural areas should be suspended for an approach that takes popular politics and political agents in rural areas seriously. The thesis finally argues that the conclusions that are reached with respect to questions of modernity and rural politics ought to be adopted to allow for more detailed and thorough explanations of popular politics in places like Ruiterbos.
- Full Text:
- Date Issued: 2019
- Authors: Ghedi Alasow, Jonis
- Date: 2019
- Subjects: Political participation -- South Africa -- Cape Town , South Africa -- Politics and government -- 1994- , Land tenure -- Political aspects -- South Africa , Land use, Rural -- Political aspects -- South Africa
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/96165 , vital:31246
- Description: This thesis argues that the philosophical foundations upon which human beings have been engaged have, across various schools of thought, made the mistake of presuming that some people are more modern than others. This suggestion is refuted throughout this thesis. To do this, intellectual traditions that take the fundamental rationality of all human beings as an indispensable starting point are engaged to argue for the need to acknowledge that everyone in the ‘now’ is indeed, of the ‘now’. This thesis connects these debates about modernity, rationality and humanity to the contemporary discussions around rural politics with particular reference to Ruiterbos in the Western Cape province of South Africa. By means of detailed empirical and ethnographic research, this thesis illustrates the issues around which people in Ruiterbos are politicised. Via this case study, the a priori assumption that rural politics will necessarily manifest itself only with respect to questions of land and agrarian reform or labour relations is complicated. The two issues around which people in Ruiterbos, during the time of this research, seem to be politicised – housing and education – are surfaced throughout this thesis. The thesis argues that the findings in this case study call for an expansion of the issues that are traditionally considered when the question of rural politics is raised. The often historicist approach that limits the possibilities for politics in rural areas should be suspended for an approach that takes popular politics and political agents in rural areas seriously. The thesis finally argues that the conclusions that are reached with respect to questions of modernity and rural politics ought to be adopted to allow for more detailed and thorough explanations of popular politics in places like Ruiterbos.
- Full Text:
- Date Issued: 2019
Queens, pseudoqueens and laying workers reproductive competition in the Cape Honeybee (Apis mellifera capensis Eschscholtz)
- Authors: Muerrle, Thomas Martin
- Date: 2008
- Subjects: Honeybee Honeybee -- Reproduction
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5750 , http://hdl.handle.net/10962/d1005437
- Description: In honeybees (Apis mellifera L.) the queen monopolises reproduction. However, especially after queen loss, workers can lay eggs, but are unable to mate. They produce haploid male offspring (drones) from unfertilised eggs via arrhenotokous parthenogenesis. In contrast, workers of the honeybee subspecies Apis mellifera capensis Eschscholtz typically produce diploid female offspring from unfertilised eggs thelytokously. After queen loss and without queen-derived brood A. m. capensis colonies can successfully requeen from worker-derived brood. This, however, is a relatively rare event in wild populations. Moreover, workerderived queens were described to be smaller, more worker-like and reproductively inferior. On the other hand, the fixation of the thelytokous trait relies mainly on sufficient numbers of viable drones produced by worker-derived queens. Small numbers of reproductively inferior worker-derived queens in A. m. capensis populations would be clearly counterintuitive. It is therefore necessary to quantify the significance of worker-dependant queen rearing pathways on the individual (queen) and on population level.Reproductive inferiority of worker-derived queens could not be confirmed on the individual (queen) level when comparing parameters indicating potential reproductive success of queen- and worker-derived queens. Queen- and worker-derived queens clearly showed a congruent range of reproductive performance. In queen rearing preference tests, increased acceptance of worker-derived female larvae was exactly counterbalanced by increased mortality, resulting in an equal number of eclosing virgin queens from an equal number of grafts in both test groups. Larval survival and successful eclosion is a prerequisite for a queen’s reproductive success. I found no difference in eclosion success for queen- and worker-derived virgin queens, indicating a similar potential for reproductive success in both queen types. Assessments of the developmental patterns of colonies headed by both queen and worker-derived queens in long-term experiments revealed no significant differences in reproductive success. Colonies headed by queen-derived queens and colonies headed by worker-derived queens could not be separated when comparing the different developmental pathways observed or from differences in worker-force. Reproductive dominance in A. m. capensis appeared tobe determined by a function of relative compositional and absolute quantitative pheromonal patterns, where individuals, which produce compositionally most queen-like blends in highest quantities, occupy top positions. Queen- and worker-derived virgin queens occupied intermediate positions between pseudoqueens and mated queens. However, no significant differences between the pheromonal status of queen- and worker-derived virgin queens were observed, suggesting a similar range of reproductive dominance for both queen types. In behavioural bioassays queen- and worker-derived virgin queens appeared to be similarly attractive to clustering workers and to drones in a drone congregation area, indicating no differences in potential reproductive success for queens from both origins for those parameters. The significant influence of the queen substance 9-ODA on attractiveness to workers and drones was confirmed. Rare requeening events from worker-derived female brood in queenless A.m. capensis do not satisfactorily explain the fixation of the thelytokous trait at a population level. I observed A. m. capensis worker ovipositing into empty artificial queen cell cups in queen-right colonies. The queen was confined behind a queen excluder grid in a separate compartment of the colony, to imitate reduced pheromonal flow, similar to swarming or superseding colonies. Eggs oviposited by workers in artificial queen cell cups were readily accepted for queen rearing and successful eclosion of viable virgin queens was observed. Consequently I suggested an alternative worker-dependant reproductive pathway in A. m. capensis, which was never described before: In swarming or superseding queenright colonies, laying workers may directly compete with the queen for reproductive success by ovipositing (instead of the queen) into natural queen cell cups. At a population level this reproductive tactic may result in large numbers of worker-derived queens of high reproductive quality in natural populations of A. m. capensis.
- Full Text:
- Date Issued: 2008
- Authors: Muerrle, Thomas Martin
- Date: 2008
- Subjects: Honeybee Honeybee -- Reproduction
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5750 , http://hdl.handle.net/10962/d1005437
- Description: In honeybees (Apis mellifera L.) the queen monopolises reproduction. However, especially after queen loss, workers can lay eggs, but are unable to mate. They produce haploid male offspring (drones) from unfertilised eggs via arrhenotokous parthenogenesis. In contrast, workers of the honeybee subspecies Apis mellifera capensis Eschscholtz typically produce diploid female offspring from unfertilised eggs thelytokously. After queen loss and without queen-derived brood A. m. capensis colonies can successfully requeen from worker-derived brood. This, however, is a relatively rare event in wild populations. Moreover, workerderived queens were described to be smaller, more worker-like and reproductively inferior. On the other hand, the fixation of the thelytokous trait relies mainly on sufficient numbers of viable drones produced by worker-derived queens. Small numbers of reproductively inferior worker-derived queens in A. m. capensis populations would be clearly counterintuitive. It is therefore necessary to quantify the significance of worker-dependant queen rearing pathways on the individual (queen) and on population level.Reproductive inferiority of worker-derived queens could not be confirmed on the individual (queen) level when comparing parameters indicating potential reproductive success of queen- and worker-derived queens. Queen- and worker-derived queens clearly showed a congruent range of reproductive performance. In queen rearing preference tests, increased acceptance of worker-derived female larvae was exactly counterbalanced by increased mortality, resulting in an equal number of eclosing virgin queens from an equal number of grafts in both test groups. Larval survival and successful eclosion is a prerequisite for a queen’s reproductive success. I found no difference in eclosion success for queen- and worker-derived virgin queens, indicating a similar potential for reproductive success in both queen types. Assessments of the developmental patterns of colonies headed by both queen and worker-derived queens in long-term experiments revealed no significant differences in reproductive success. Colonies headed by queen-derived queens and colonies headed by worker-derived queens could not be separated when comparing the different developmental pathways observed or from differences in worker-force. Reproductive dominance in A. m. capensis appeared tobe determined by a function of relative compositional and absolute quantitative pheromonal patterns, where individuals, which produce compositionally most queen-like blends in highest quantities, occupy top positions. Queen- and worker-derived virgin queens occupied intermediate positions between pseudoqueens and mated queens. However, no significant differences between the pheromonal status of queen- and worker-derived virgin queens were observed, suggesting a similar range of reproductive dominance for both queen types. In behavioural bioassays queen- and worker-derived virgin queens appeared to be similarly attractive to clustering workers and to drones in a drone congregation area, indicating no differences in potential reproductive success for queens from both origins for those parameters. The significant influence of the queen substance 9-ODA on attractiveness to workers and drones was confirmed. Rare requeening events from worker-derived female brood in queenless A.m. capensis do not satisfactorily explain the fixation of the thelytokous trait at a population level. I observed A. m. capensis worker ovipositing into empty artificial queen cell cups in queen-right colonies. The queen was confined behind a queen excluder grid in a separate compartment of the colony, to imitate reduced pheromonal flow, similar to swarming or superseding colonies. Eggs oviposited by workers in artificial queen cell cups were readily accepted for queen rearing and successful eclosion of viable virgin queens was observed. Consequently I suggested an alternative worker-dependant reproductive pathway in A. m. capensis, which was never described before: In swarming or superseding queenright colonies, laying workers may directly compete with the queen for reproductive success by ovipositing (instead of the queen) into natural queen cell cups. At a population level this reproductive tactic may result in large numbers of worker-derived queens of high reproductive quality in natural populations of A. m. capensis.
- Full Text:
- Date Issued: 2008
Uphuhliswano lwezikolo zaseRhini
- Authors: Badat, Saleem
- Date: 2007-02-23 , 2014-06-13
- Language: English
- Type: Text
- Identifier: vital:7625 , http://hdl.handle.net/10962/d1011845
- Description: Address at the Department of Education Grahamstown District Matric Awards, PJ Olivier High School, 23 February 2007
- Full Text:
- Date Issued: 2007-02-23
- Authors: Badat, Saleem
- Date: 2007-02-23 , 2014-06-13
- Language: English
- Type: Text
- Identifier: vital:7625 , http://hdl.handle.net/10962/d1011845
- Description: Address at the Department of Education Grahamstown District Matric Awards, PJ Olivier High School, 23 February 2007
- Full Text:
- Date Issued: 2007-02-23
Recovery and molecular identification of Aichi virus 1, enteric human bocaviruses and enteric human adenoviruses in untreated sewage and mussel samples collected in the Eastern Cape Province of South Africa
- Authors: Onosi, Oikwathaile
- Date: 2019
- Subjects: Sewage -- Analysis -- South Africa -- Eastern Cape , Sewage -- Microbiology -- South Africa -- Eastern Cape , Viral pollution of water -- South Africa -- Eastern Cape , Sewage disposal in rivers, lakes, etc. -- South Africa -- Eastern Cape , Enteroviruses -- South Africa -- Eastern Cape , Picornaviruses -- South Africa -- Eastern Cape , Aichi virus 1 , Parvoviruses -- South Africa -- Eastern Cape , Adenoviruses -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/69456 , vital:29539
- Description: Gastroenteritis, commonly known as diarrhoeal disease, is one of the top killers responsible for substantial human morbidity and mortality especially in third world countries where most people do not have access to potable water and where hygiene levels are low. Many bacterial, viral and protozoal agents are known causes of gastroenteritis and viral gastroenteritis is responsible for over 70% of cases. Rotaviruses are the main causes of viral gastroenteritis and are responsible for most of the cases worldwide. Other viral agents associated with this disease include human noroviruses, Aichi virus 1, enteric human bocavirus, enteric human adenovirus and many other emerging viral agents such as klassivirus, Saffold virus, cosavirus and others. In 2009 the South African government introduced a rotavirus vaccine, RotaRixTM into the expanded programme on immunisation (EPI). More than a 50% decrease in diarrhoea related morbidity and mortality due to rotavirus infections was noted during surveillance studies on the efficacy of the vaccine. However, over 40% of cases of gastroenteritis are of unknown aetiology. The present study aimed to perform a preliminary study to investigate the presence of Aichi virus 1 and enteric human bocaviruses in the Eastern Cape Province by the use of molecular techniques. Furthermore, the study aimed to add to the limited molecular data about enteric adenoviruses in South Africa. Samples used in this study were swab samples collected from Belmont Valley Wastewater Treatment Plant in Grahamstown, South Africa, as well as mussel samples collected from the Swartkops River in Port Elizabeth, South Africa. Both raw sewage and shellfish give a broad idea of what microbes are circulating in the communities. In the present study, twenty swabs and twenty mussel samples were prepared by centrifugation, sonication and filtration. Samples were then subjected to transmission electron microscopy (TEM) analysis, for which the electron micrographs revealed presence of viral particles with diameters ranging from around 20 nm to just over 100 nm. Viral nucleic acids were extracted from 140 μL of the twenty swabs and twenty mussels samples using the QIAamp® Viral RNA Mini Kit, following manufacturer‟s instructions. For detection of Aichi virus 1 from the swab and mussel samples three reverse transcriptase- polymerase chain reaction (RT-PCR) assays using the Verso 1-Step RT-PCR Hot-Start Kit were developed. The first RT-PCR assay targeted amplification of the highly conserved 5′ UTR using published primers. However, despite many amplification attempts no positive results were obtained from both swab and mussel samples. It was only after the addition of DMSO (to a final concentration of 10%) that one swab sample was positive for this assay. In addition, a 2-step RT-PCR was developed using the Maxima H Minus First Strand cDNA Synthesis Kit. By using this 2-step RT-PCR assay, an additional swab sample was positive for the Aichi virus 1 5′ UTR. Using Basic Logarithm Alignment Search Tool (BLAST) analysis these two samples were 98% identical to an Aichi virus isolate from South Korea. The second one-step RT-PCR assay targeted amplification of the 266 bp partial 3CD coding region of Aichi virus 1 using published primers. By using this assay, positive results were obtained from both the swab and mussel samples, which when analysed by BLAST were all 99% identical to various Aichi virus 1 isolates in GenBank. A phylogenetic tree constructed based on this region showed that isolates from the present study clustered with Genotype B isolates in GenBank. The third assay was a semi-nested RT-PCR assay that targeted amplification of the hypervariable VP1 coding region of Aichi virus 1 using a combination of published primers and those designed in the present study. Amplicons which were 472 bp in size were produced from two swab samples. When analysed by BLAST, these two swab samples had percentage identities of 98% to an Aichi virus isolate from South Korea. A phylogenetic tree constructed based on this region showed that isolates from the present study clustered with Genotype B isolates in GenBank. This was consistent with phylogenetic results discussed above which were based on the partial 3CD region. For detection of enteric human bocaviruses from the swab and mussel samples a nested polymerase chain reaction (PCR) assay, using the Ampliqon Taq PCR kit (Ampliqon Bio Reagents and Molecular Diagnostics, Denmark) was developed based on PCR amplification of the 382 bp partial VP1/VP2 coding region using published primers. A total of six swab samples and six mussel samples were analysed for which five swabs and six mussel samples gave positive results. When analysed by BLAST, the swab samples had percentage identities of between 98% and 99% to an enteric human bocavirus 3 strain from China while the mussel samples were all 99% identical to an enteric human bocavirus 2 isolate from Australia. A phylogenetic tree constructed based on this VP1/VP2 region showed that isolates from the present study clustered with human bocavirus 2 and human bocavirus 3 isolates in GenBank for those isolated from swab samples and mussel samples respectively. Lastly, for detection of enteric human adenoviruses from the swab and mussel samples a nested PCR assay, using the Ampliqon Taq PCR kit (Ampliqon Bio Reagents and Molecular Diagnostics, Denmark) was developed. This reaction was based on PCR amplification of the 168 bp partial hexon coding region using published primers for which ten swab samples gave positive results. When analysed by BLAST, the swab samples had percentage identities of between 96% and 99% to enteric human adenoviruses in GenBank. A phylogenetic tree constructed based on the hexon coding region showed that isolates from the present study clustered with subtypes C, D and F which are associated with gastroenteritis worldwide. Despite several amplification attempts no positive results were obtained from mussel samples. The results from the present study show that Aichi virus 1, enteric bocaviruses and enteric adenoviruses are present in the Eastern Cape Province of South Africa. These viruses could possibly be responsible for enteric infections in South Africa. Although only a few samples were analysed, this study is the first to confirm the presence of Aichi virus 1 and enteric bocaviruses in South Africa and provides a platform for further investigation into prevalence and epidemiology of these viruses in the country.
- Full Text:
- Date Issued: 2019
- Authors: Onosi, Oikwathaile
- Date: 2019
- Subjects: Sewage -- Analysis -- South Africa -- Eastern Cape , Sewage -- Microbiology -- South Africa -- Eastern Cape , Viral pollution of water -- South Africa -- Eastern Cape , Sewage disposal in rivers, lakes, etc. -- South Africa -- Eastern Cape , Enteroviruses -- South Africa -- Eastern Cape , Picornaviruses -- South Africa -- Eastern Cape , Aichi virus 1 , Parvoviruses -- South Africa -- Eastern Cape , Adenoviruses -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/69456 , vital:29539
- Description: Gastroenteritis, commonly known as diarrhoeal disease, is one of the top killers responsible for substantial human morbidity and mortality especially in third world countries where most people do not have access to potable water and where hygiene levels are low. Many bacterial, viral and protozoal agents are known causes of gastroenteritis and viral gastroenteritis is responsible for over 70% of cases. Rotaviruses are the main causes of viral gastroenteritis and are responsible for most of the cases worldwide. Other viral agents associated with this disease include human noroviruses, Aichi virus 1, enteric human bocavirus, enteric human adenovirus and many other emerging viral agents such as klassivirus, Saffold virus, cosavirus and others. In 2009 the South African government introduced a rotavirus vaccine, RotaRixTM into the expanded programme on immunisation (EPI). More than a 50% decrease in diarrhoea related morbidity and mortality due to rotavirus infections was noted during surveillance studies on the efficacy of the vaccine. However, over 40% of cases of gastroenteritis are of unknown aetiology. The present study aimed to perform a preliminary study to investigate the presence of Aichi virus 1 and enteric human bocaviruses in the Eastern Cape Province by the use of molecular techniques. Furthermore, the study aimed to add to the limited molecular data about enteric adenoviruses in South Africa. Samples used in this study were swab samples collected from Belmont Valley Wastewater Treatment Plant in Grahamstown, South Africa, as well as mussel samples collected from the Swartkops River in Port Elizabeth, South Africa. Both raw sewage and shellfish give a broad idea of what microbes are circulating in the communities. In the present study, twenty swabs and twenty mussel samples were prepared by centrifugation, sonication and filtration. Samples were then subjected to transmission electron microscopy (TEM) analysis, for which the electron micrographs revealed presence of viral particles with diameters ranging from around 20 nm to just over 100 nm. Viral nucleic acids were extracted from 140 μL of the twenty swabs and twenty mussels samples using the QIAamp® Viral RNA Mini Kit, following manufacturer‟s instructions. For detection of Aichi virus 1 from the swab and mussel samples three reverse transcriptase- polymerase chain reaction (RT-PCR) assays using the Verso 1-Step RT-PCR Hot-Start Kit were developed. The first RT-PCR assay targeted amplification of the highly conserved 5′ UTR using published primers. However, despite many amplification attempts no positive results were obtained from both swab and mussel samples. It was only after the addition of DMSO (to a final concentration of 10%) that one swab sample was positive for this assay. In addition, a 2-step RT-PCR was developed using the Maxima H Minus First Strand cDNA Synthesis Kit. By using this 2-step RT-PCR assay, an additional swab sample was positive for the Aichi virus 1 5′ UTR. Using Basic Logarithm Alignment Search Tool (BLAST) analysis these two samples were 98% identical to an Aichi virus isolate from South Korea. The second one-step RT-PCR assay targeted amplification of the 266 bp partial 3CD coding region of Aichi virus 1 using published primers. By using this assay, positive results were obtained from both the swab and mussel samples, which when analysed by BLAST were all 99% identical to various Aichi virus 1 isolates in GenBank. A phylogenetic tree constructed based on this region showed that isolates from the present study clustered with Genotype B isolates in GenBank. The third assay was a semi-nested RT-PCR assay that targeted amplification of the hypervariable VP1 coding region of Aichi virus 1 using a combination of published primers and those designed in the present study. Amplicons which were 472 bp in size were produced from two swab samples. When analysed by BLAST, these two swab samples had percentage identities of 98% to an Aichi virus isolate from South Korea. A phylogenetic tree constructed based on this region showed that isolates from the present study clustered with Genotype B isolates in GenBank. This was consistent with phylogenetic results discussed above which were based on the partial 3CD region. For detection of enteric human bocaviruses from the swab and mussel samples a nested polymerase chain reaction (PCR) assay, using the Ampliqon Taq PCR kit (Ampliqon Bio Reagents and Molecular Diagnostics, Denmark) was developed based on PCR amplification of the 382 bp partial VP1/VP2 coding region using published primers. A total of six swab samples and six mussel samples were analysed for which five swabs and six mussel samples gave positive results. When analysed by BLAST, the swab samples had percentage identities of between 98% and 99% to an enteric human bocavirus 3 strain from China while the mussel samples were all 99% identical to an enteric human bocavirus 2 isolate from Australia. A phylogenetic tree constructed based on this VP1/VP2 region showed that isolates from the present study clustered with human bocavirus 2 and human bocavirus 3 isolates in GenBank for those isolated from swab samples and mussel samples respectively. Lastly, for detection of enteric human adenoviruses from the swab and mussel samples a nested PCR assay, using the Ampliqon Taq PCR kit (Ampliqon Bio Reagents and Molecular Diagnostics, Denmark) was developed. This reaction was based on PCR amplification of the 168 bp partial hexon coding region using published primers for which ten swab samples gave positive results. When analysed by BLAST, the swab samples had percentage identities of between 96% and 99% to enteric human adenoviruses in GenBank. A phylogenetic tree constructed based on the hexon coding region showed that isolates from the present study clustered with subtypes C, D and F which are associated with gastroenteritis worldwide. Despite several amplification attempts no positive results were obtained from mussel samples. The results from the present study show that Aichi virus 1, enteric bocaviruses and enteric adenoviruses are present in the Eastern Cape Province of South Africa. These viruses could possibly be responsible for enteric infections in South Africa. Although only a few samples were analysed, this study is the first to confirm the presence of Aichi virus 1 and enteric bocaviruses in South Africa and provides a platform for further investigation into prevalence and epidemiology of these viruses in the country.
- Full Text:
- Date Issued: 2019
The theory of economic underdevelopment and its applicability to the Rhodesian economy
- Authors: Clarke, Duncan G, 1948-
- Date: 1969
- Subjects: Zimbabwe -- Economic conditions , Economic development -- Zimbabwe , Developing countries -- Economic conditions
- Language: English
- Type: Thesis , Honours , BCom
- Identifier: vital:1114 , http://hdl.handle.net/10962/d1014691
- Description: According to the canons of conventional economic philosophy the process of economic interpretation should be value neutral and strictly fall within the bounds of normative science. This approach is concerned not with goal setting but only with the technical possibilities of alternative means of successful tactics in a given overall strategy. It is the author's thesis that such premises patently ignore the fundamental truths of development problems, and that there exists a genuine need to bridge the gap that demarcates theory from practicality and truth from illusion. To seek "development" implies a challenge to the "status quo" of menial existence and perpetual servitude to the inhospitable forces of ones own environment. This attitude is in itself a value judgement, and in underdeveloped societies it is more than a mere academic quibble. Accordingly, this paper not only implicitly assumes "development" to be a desirable goal but also that it is necessary, and the objective of this study of an underdeveloped community shall be to examine the theoretical relevance, or otherwise, of general and partial theories of underdevelopment against the quantitative and qualitative evidence of the course of events that have in the past, and are likely in the future, to influence the development of the "Rhodesian economy".
- Full Text:
- Date Issued: 1969
- Authors: Clarke, Duncan G, 1948-
- Date: 1969
- Subjects: Zimbabwe -- Economic conditions , Economic development -- Zimbabwe , Developing countries -- Economic conditions
- Language: English
- Type: Thesis , Honours , BCom
- Identifier: vital:1114 , http://hdl.handle.net/10962/d1014691
- Description: According to the canons of conventional economic philosophy the process of economic interpretation should be value neutral and strictly fall within the bounds of normative science. This approach is concerned not with goal setting but only with the technical possibilities of alternative means of successful tactics in a given overall strategy. It is the author's thesis that such premises patently ignore the fundamental truths of development problems, and that there exists a genuine need to bridge the gap that demarcates theory from practicality and truth from illusion. To seek "development" implies a challenge to the "status quo" of menial existence and perpetual servitude to the inhospitable forces of ones own environment. This attitude is in itself a value judgement, and in underdeveloped societies it is more than a mere academic quibble. Accordingly, this paper not only implicitly assumes "development" to be a desirable goal but also that it is necessary, and the objective of this study of an underdeveloped community shall be to examine the theoretical relevance, or otherwise, of general and partial theories of underdevelopment against the quantitative and qualitative evidence of the course of events that have in the past, and are likely in the future, to influence the development of the "Rhodesian economy".
- Full Text:
- Date Issued: 1969
Cranial ontogeny of the sole, dagetichthys marginatus (soleidae), with considerations on the feeding ability of larvae and early juveniles
- Ende, Stephan Siegfried Werner
- Authors: Ende, Stephan Siegfried Werner
- Date: 2008
- Subjects: Soleidae , Fishes -- Larvae -- Food , Flatfishes -- Nutrition , Artemia
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5250 , http://hdl.handle.net/10962/d1005093 , Soleidae , Fishes -- Larvae -- Food , Flatfishes -- Nutrition , Artemia
- Description: The overall aim of this study was to gain a better understanding of the feeding mechanisms of the larvae and early juveniles of the sole, Dagetichthys marginatus (Soleidae), with which to assess the suitability of current feeding protocols and to facilitate the development of an appropriate pelleted feed. This was achieved by examining the ontogeny of the cranium of laboratory reared sole, with particular emphasis on those elements associated with feeding and by comparing the cranium of juvenile fish with that of adult fish. At 4 dah (days after hatch) Dagetichthys marginatus larvae develop the first rudimentary branchial arches that facilitates the capture and ingestion of food items. Subsequent development of cranial structures, such as the oral jaws, suspensorium, neurocranium, hyoid and branchial arches and the opercular apparatus enables the larvae, at 16 dah, to switch from ram feeding to suction feeding on live prey. The use of live Artemia nauplii from 4 to 16 dah is therefore appropriate. The first morphological asymmetries developed at 16 dah in the dentaries and at 22 dah the maxillae and the premaxillae began to show asymmetries. Teeth were present only on the blind side of the oral jaw elements and during this period (16 to 22 dah) the existing elements began to ossify. From 16 to 35 dah the standard feeding protocol consists of a combination of pelagic (Artemia metanauplii) and benthic prey (dead, frozen Artemia nauplii) and from 25 dah onwards a sinking pellet is provided. The time (dah) at which frozen Artemia and sinking pellets were provided, appropriately corresponded to the initiation of benthic feeding behaviour. However at this stage the use of pelagic Artemia metanauplii is inappropriate and unnecessary. At 31 dah the cranial morphology resembled that of adult fish. Adult D. marginatus display extreme asymmetries among the elements of the oral jaws, the suspensorium and certain elements of the neurocranium. Elements on the blind side are larger and more robust than those on the ocular side and are adapted for feeding, while those on the ocular side appear to have a respiratory function. From 31 dah the cranial elements are identical to those of adult fish, suggesting that no further feeding behavioural changes occurred and that a sinking pellet, of which the nutrient composition meets the requirements of the fish, would be appropriate for ongrowing.
- Full Text:
- Date Issued: 2008
- Authors: Ende, Stephan Siegfried Werner
- Date: 2008
- Subjects: Soleidae , Fishes -- Larvae -- Food , Flatfishes -- Nutrition , Artemia
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5250 , http://hdl.handle.net/10962/d1005093 , Soleidae , Fishes -- Larvae -- Food , Flatfishes -- Nutrition , Artemia
- Description: The overall aim of this study was to gain a better understanding of the feeding mechanisms of the larvae and early juveniles of the sole, Dagetichthys marginatus (Soleidae), with which to assess the suitability of current feeding protocols and to facilitate the development of an appropriate pelleted feed. This was achieved by examining the ontogeny of the cranium of laboratory reared sole, with particular emphasis on those elements associated with feeding and by comparing the cranium of juvenile fish with that of adult fish. At 4 dah (days after hatch) Dagetichthys marginatus larvae develop the first rudimentary branchial arches that facilitates the capture and ingestion of food items. Subsequent development of cranial structures, such as the oral jaws, suspensorium, neurocranium, hyoid and branchial arches and the opercular apparatus enables the larvae, at 16 dah, to switch from ram feeding to suction feeding on live prey. The use of live Artemia nauplii from 4 to 16 dah is therefore appropriate. The first morphological asymmetries developed at 16 dah in the dentaries and at 22 dah the maxillae and the premaxillae began to show asymmetries. Teeth were present only on the blind side of the oral jaw elements and during this period (16 to 22 dah) the existing elements began to ossify. From 16 to 35 dah the standard feeding protocol consists of a combination of pelagic (Artemia metanauplii) and benthic prey (dead, frozen Artemia nauplii) and from 25 dah onwards a sinking pellet is provided. The time (dah) at which frozen Artemia and sinking pellets were provided, appropriately corresponded to the initiation of benthic feeding behaviour. However at this stage the use of pelagic Artemia metanauplii is inappropriate and unnecessary. At 31 dah the cranial morphology resembled that of adult fish. Adult D. marginatus display extreme asymmetries among the elements of the oral jaws, the suspensorium and certain elements of the neurocranium. Elements on the blind side are larger and more robust than those on the ocular side and are adapted for feeding, while those on the ocular side appear to have a respiratory function. From 31 dah the cranial elements are identical to those of adult fish, suggesting that no further feeding behavioural changes occurred and that a sinking pellet, of which the nutrient composition meets the requirements of the fish, would be appropriate for ongrowing.
- Full Text:
- Date Issued: 2008
Structure and interaction studies of beta-amyloid in the search for new lead compounds for the treatment of Alzheimer’s disease
- Authors: Mtini, Onke
- Date: 2020
- Subjects: Alzheimer's disease -- Chemotherapy , Alzheimer's disease -- Treatment , Amyloid beta-protein , Oxidative stress , Protein binding , South African Natural Compounds Database
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/167574 , vital:41493
- Description: Alzheimer’s disease (AD) is the most devastating neurodegenerative disorder that effects the aging population worldwide. In this study three hypotheses of AD are explored, the β-amyloid cascade hypothesis, the β-amyloid metal binding hypothesis and the oxidative stress hypothesis are explored. In the first case compounds from the South African Natural Compounds Database (SANCDB) are docked to models of β-amyloid fibrils and the properties of these fibrils under pulling simulations are compared to a known small molecule disruptor of β-amyloid, wgx-50. In these simulations SANCDB compounds are identified that disrupt β-amyloid in a similar manner to wgx-50. In these simulations the disruption to the free energy of binding of chains to the fibrils is quantified. For metal binding and oxidative stress hypotheses, problems in simulation arise due to only fragments of β-amyloid being present in the Research Collaboratory for Structural Bioinformatics protein data bank (RCSB PDB), as determined from NMR experiments. In this work, β-amyloid is set up under periodic boundary conditions to simulate a fibril under reasonable computational time. Within these periodic boundary conditions, β-amyloid has been solvated in copper and zinc rich environments and diffusion of these metals around the fibrils has been explored. The localization of these metals (in simulation only using van der Waal’s and electrostatic terms) around the fibril has led us to explore other possible metal binding sites. Metal bound to the infinite fibril has been optimized at the QM/MM level and some of the reactive oxygen species in the presence of the fibril are quantified.
- Full Text:
- Date Issued: 2020
- Authors: Mtini, Onke
- Date: 2020
- Subjects: Alzheimer's disease -- Chemotherapy , Alzheimer's disease -- Treatment , Amyloid beta-protein , Oxidative stress , Protein binding , South African Natural Compounds Database
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/167574 , vital:41493
- Description: Alzheimer’s disease (AD) is the most devastating neurodegenerative disorder that effects the aging population worldwide. In this study three hypotheses of AD are explored, the β-amyloid cascade hypothesis, the β-amyloid metal binding hypothesis and the oxidative stress hypothesis are explored. In the first case compounds from the South African Natural Compounds Database (SANCDB) are docked to models of β-amyloid fibrils and the properties of these fibrils under pulling simulations are compared to a known small molecule disruptor of β-amyloid, wgx-50. In these simulations SANCDB compounds are identified that disrupt β-amyloid in a similar manner to wgx-50. In these simulations the disruption to the free energy of binding of chains to the fibrils is quantified. For metal binding and oxidative stress hypotheses, problems in simulation arise due to only fragments of β-amyloid being present in the Research Collaboratory for Structural Bioinformatics protein data bank (RCSB PDB), as determined from NMR experiments. In this work, β-amyloid is set up under periodic boundary conditions to simulate a fibril under reasonable computational time. Within these periodic boundary conditions, β-amyloid has been solvated in copper and zinc rich environments and diffusion of these metals around the fibrils has been explored. The localization of these metals (in simulation only using van der Waal’s and electrostatic terms) around the fibril has led us to explore other possible metal binding sites. Metal bound to the infinite fibril has been optimized at the QM/MM level and some of the reactive oxygen species in the presence of the fibril are quantified.
- Full Text:
- Date Issued: 2020
Narratiewe strategieë by die ondersoek van die verlede in twee romans, naamlik Lijken op liefde (1997) deur Astrid H. Roemer en Duiwelskloof (1998) deur André P. Brink
- Authors: Potgieter, Maretha
- Date: 2002
- Subjects: Roemer, Astrid. Lijken op liefde , Brink, André Philippus, 1935- Duiwelskloof
- Language: Afrikaans
- Type: Thesis , Masters , MA
- Identifier: vital:3578 , http://hdl.handle.net/10962/d1002151 , Roemer, Astrid. Lijken op liefde , Brink, André Philippus, 1935- Duiwelskloof
- Description: This study investigates some of the narrative strategies used by two novels, namely Lijken op Liefde (1997) by the Surinam born author Astrid H. Roemer and Duiwelskloof (1998) by the South African André P. Brink, in their exploration of and dialogue with traumatic pasts. Both texts are written within so-called postcolonial time frames, delve into the personal and collective past and attempt to provide a corrective on knowledges that used to be deemed not useful, unimportant or have been forgotten by official historiography. The theoretical base of the study is an eclectic mixture of both postcolonial and postmodern theories, throwing light on the strategies employed by these novels in order to understand, problematise and creatively exploit the past. , Hierdie studie kyk na sommige van die narratiewe strategieë binne twee romans, naamlik Lijken op Liefde (1997) deur die Surinaams-gebore outeur Astrid H. Roemer en Duiwelskloof (1998) deur die Suid-Afrikaner André P. Brink, in hulle ondersoek na en dialoog met die traumatiese verlede. Beide tekste is geskryf binne sogenaamde postkoloniale tydvakke, delf in die persoonlike en kollektiewe verlede, en verskaf ’n korrektief op die dinge wat as onbruikbaar of onbelangrik beskou is, of vergete geraak het binne die offisiële historiografie. Die teoretiese basis van die studie is ’n eklektiese mengsel van beide postkoloniale en postmoderne teorieë wat lig werp op die strategieë wat hierdie tekste gebruik om die verlede te verstaan, te problematiseer en kreatief te ontgin.
- Full Text:
- Date Issued: 2002
- Authors: Potgieter, Maretha
- Date: 2002
- Subjects: Roemer, Astrid. Lijken op liefde , Brink, André Philippus, 1935- Duiwelskloof
- Language: Afrikaans
- Type: Thesis , Masters , MA
- Identifier: vital:3578 , http://hdl.handle.net/10962/d1002151 , Roemer, Astrid. Lijken op liefde , Brink, André Philippus, 1935- Duiwelskloof
- Description: This study investigates some of the narrative strategies used by two novels, namely Lijken op Liefde (1997) by the Surinam born author Astrid H. Roemer and Duiwelskloof (1998) by the South African André P. Brink, in their exploration of and dialogue with traumatic pasts. Both texts are written within so-called postcolonial time frames, delve into the personal and collective past and attempt to provide a corrective on knowledges that used to be deemed not useful, unimportant or have been forgotten by official historiography. The theoretical base of the study is an eclectic mixture of both postcolonial and postmodern theories, throwing light on the strategies employed by these novels in order to understand, problematise and creatively exploit the past. , Hierdie studie kyk na sommige van die narratiewe strategieë binne twee romans, naamlik Lijken op Liefde (1997) deur die Surinaams-gebore outeur Astrid H. Roemer en Duiwelskloof (1998) deur die Suid-Afrikaner André P. Brink, in hulle ondersoek na en dialoog met die traumatiese verlede. Beide tekste is geskryf binne sogenaamde postkoloniale tydvakke, delf in die persoonlike en kollektiewe verlede, en verskaf ’n korrektief op die dinge wat as onbruikbaar of onbelangrik beskou is, of vergete geraak het binne die offisiële historiografie. Die teoretiese basis van die studie is ’n eklektiese mengsel van beide postkoloniale en postmoderne teorieë wat lig werp op die strategieë wat hierdie tekste gebruik om die verlede te verstaan, te problematiseer en kreatief te ontgin.
- Full Text:
- Date Issued: 2002
Heterologous expression of the helicoverpa armigera stunt virus in Saccharomyces cerevisiae
- Authors: Venter, Philip Arno
- Date: 2002
- Subjects: Helicoverpa armigera Saccharomyces cerevisiae
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3895 , http://hdl.handle.net/10962/d1003811
- Description: Lepidopteran insects like Helicoverpa armigera, more commonly known as the cotton bollworm, are economically important pests of a wide variety of crops throughout the world. The Helicoverpa armigera stunt virus (HaSV), a tetravirus with a bipartite single-stranded positive-sense RNA genome, has great potential as a biological pesticide against H. armigera. The larger genomic strand of this virus (RNA1) encodes the viral replicase, while the other (RNA2) encodes the 71 kDa capsid protein precursor (p71). 240 copies of p71 assemble into a procapsid with the concomitant encapsidation of the viral RNA. This is followed by a complex maturation event that is characterized by the autoproteolytic cleavage of p71 into the 64 kDa capsid protein (P64) and a 7 kDa peptide (p7). The rearrangements that occur during maturation results in the formation of mature HaSV capsids that can thereupon deliver RNA to other susceptible host cells. The principal objective of the research described in this study was to demonstrate that this virus could be assembled in Saccharomyces cerevisiae. S. cerevisiae expression vectors were constructed for the production of p71. This protein was detected in cell lysates from two different strains of S. cerevisiae, both containing either chromosomal or episomal copies of an expression cassette for P71. A number of factors relating to the expression of P71 (e.g. strains used, expression loci and expression rate) and the preparation of protein extracts from S. cerevisiae (e.g. the presence of various protease inhibitors and salt concentrations) were examined to attain optimal levels of soluble p71. A small fraction of the optimized soluble p71 was shown to be in the form of virus-like particles (VLPs), with a yield of ≤10⁷ VLPs from a 1.5l culture of P71⁺ cells. These particles were exclusively in the procapsid form, had a similar buoyant density to that of wild-type HaSV and could undergo maturation when the pH was reduced to 5. S. cerevisiae vectors were constructed for the episomal expression of the HaSV genomic RNAs. These vectors directed the transcription of RNA1 and RNA2 transcripts, which had similar sizes to those of the HaSV genomic RNAs. Mature HaSV particles were purified from cells, transgenic for P71, RNA1 and RNA2, by way of two different virus purification protocols that were developed during this study. RT-PCR analyses on RNA-extracts from these particles demonstrated that RNA transcripts, which were produced in trans with p71, could be encapsidated by HaSV capsids in S. cerevisiae. A droplet-feed bioassay on H. armigera larvae demonstrated that the S. cerevisiae-derived HaSV particles caused impaired larval development. This response was correlated with the detection of HaSV RNA2 in RNA extractions from larvae that were used in this bioassay. The results that were generated through the course of this study, provided proof for the concept of the non-host production of infectious HaSV particles from S. cerevisiae. This work could serve as a foundation for future research on the development of an expression system for the large-scale production of this virus as a biopesticide.
- Full Text:
- Date Issued: 2002
- Authors: Venter, Philip Arno
- Date: 2002
- Subjects: Helicoverpa armigera Saccharomyces cerevisiae
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3895 , http://hdl.handle.net/10962/d1003811
- Description: Lepidopteran insects like Helicoverpa armigera, more commonly known as the cotton bollworm, are economically important pests of a wide variety of crops throughout the world. The Helicoverpa armigera stunt virus (HaSV), a tetravirus with a bipartite single-stranded positive-sense RNA genome, has great potential as a biological pesticide against H. armigera. The larger genomic strand of this virus (RNA1) encodes the viral replicase, while the other (RNA2) encodes the 71 kDa capsid protein precursor (p71). 240 copies of p71 assemble into a procapsid with the concomitant encapsidation of the viral RNA. This is followed by a complex maturation event that is characterized by the autoproteolytic cleavage of p71 into the 64 kDa capsid protein (P64) and a 7 kDa peptide (p7). The rearrangements that occur during maturation results in the formation of mature HaSV capsids that can thereupon deliver RNA to other susceptible host cells. The principal objective of the research described in this study was to demonstrate that this virus could be assembled in Saccharomyces cerevisiae. S. cerevisiae expression vectors were constructed for the production of p71. This protein was detected in cell lysates from two different strains of S. cerevisiae, both containing either chromosomal or episomal copies of an expression cassette for P71. A number of factors relating to the expression of P71 (e.g. strains used, expression loci and expression rate) and the preparation of protein extracts from S. cerevisiae (e.g. the presence of various protease inhibitors and salt concentrations) were examined to attain optimal levels of soluble p71. A small fraction of the optimized soluble p71 was shown to be in the form of virus-like particles (VLPs), with a yield of ≤10⁷ VLPs from a 1.5l culture of P71⁺ cells. These particles were exclusively in the procapsid form, had a similar buoyant density to that of wild-type HaSV and could undergo maturation when the pH was reduced to 5. S. cerevisiae vectors were constructed for the episomal expression of the HaSV genomic RNAs. These vectors directed the transcription of RNA1 and RNA2 transcripts, which had similar sizes to those of the HaSV genomic RNAs. Mature HaSV particles were purified from cells, transgenic for P71, RNA1 and RNA2, by way of two different virus purification protocols that were developed during this study. RT-PCR analyses on RNA-extracts from these particles demonstrated that RNA transcripts, which were produced in trans with p71, could be encapsidated by HaSV capsids in S. cerevisiae. A droplet-feed bioassay on H. armigera larvae demonstrated that the S. cerevisiae-derived HaSV particles caused impaired larval development. This response was correlated with the detection of HaSV RNA2 in RNA extractions from larvae that were used in this bioassay. The results that were generated through the course of this study, provided proof for the concept of the non-host production of infectious HaSV particles from S. cerevisiae. This work could serve as a foundation for future research on the development of an expression system for the large-scale production of this virus as a biopesticide.
- Full Text:
- Date Issued: 2002
Victoria Mounted Troops
- Type: Image
- Identifier: vital:14227 , http://hdl.handle.net/10962/d1018151 , MS 10 542 , CD 55
- Description: Troops participating in the celebration of Queen Victoria’s Diamond Jubilee 1897.
- Full Text: false
- Type: Image
- Identifier: vital:14227 , http://hdl.handle.net/10962/d1018151 , MS 10 542 , CD 55
- Description: Troops participating in the celebration of Queen Victoria’s Diamond Jubilee 1897.
- Full Text: false
Teachers’ narratives of their experience of teaching Fetal Alcohol Spectrum Disorders (FASD) -affected children in mainstream schooling
- Authors: Makasi-Simukonda, Mihlali
- Date: 2022-10-14
- Subjects: Fetal alcohol spectrum disorders South Africa Buffalo City , Inclusive education South Africa Buffalo City , Teachers of children with disabilities South Africa Buffalo City , Children of prenatal alcohol abuse South Africa Buffalo City Social conditions , Social constructionism
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406183 , vital:70246
- Description: This study considers the experiences of teachers working with Fetal Alcohol Spectrum Disorders (FASD)-affected children in mainstream schooling. FASD refers to a range of conditions in children resulting from maternal alcohol consumption during pregnancy. Prevalence rates in South Africa are said to be among the highest in the world. There is a paucity of research on FASD-affected children in the Eastern Cape. This study was thus conducted in the Buffalo City Metropolitan Municipality (BCMM). Teachers told stories of their experiences of teaching undiagnosed FASD-affected children in mainstream schooling. The stories reveal the quality of life for children in classrooms and socio-contextual influences of their experiences at school, as well as the responsibilisation of teachers in these settings. This qualitative research is situated within a social constructionism paradigm. The social model of disability was utilised as a theoretical framework for this study to represent the systemic barriers against which FASD-affected learner support in the mainstream school can be conceptualised. A sample of five (5) Black female teachers were interviewed. Data gathering was done by conducting telephonic interviews using the single question inducing narrative, known as SQUIN. A thematic analysis of the data was conducted for the purpose of identifying themes within the data collected. Four superordinate themes emerged from the data, viz. narratives of FASD-affected learners, narratives of parental involvement, narratives of the role of teachers and narratives of stakeholder support. This study reveals a significant level of responsibilisation of teachers. Teachers appear to play a bigger role than just being teachers and they positioned themselves as playing a parental role in relation to FASD-affected learners. FASD-affected learners were positioned as “the problem” in the mainstream classroom whereas the environment is a disabling factor to FASD-affected learners. There is a need for a holistic approach in working with FASD-affected learners from all relevant stakeholders. Key recommendations are made for effective inclusion of FASD-affected learners in mainstream schooling. Future research recommendations include repeating this study in a different geographical area, a focus on senior phase learners and/or a more diverse sample. The findings assist in the development of policies of inclusive education in mainstream schooling and supportive strategies to enhance the developmental trajectory of FASD-affected children. , Thesis (MA) -- Faculty of Humanities, Psychology, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Makasi-Simukonda, Mihlali
- Date: 2022-10-14
- Subjects: Fetal alcohol spectrum disorders South Africa Buffalo City , Inclusive education South Africa Buffalo City , Teachers of children with disabilities South Africa Buffalo City , Children of prenatal alcohol abuse South Africa Buffalo City Social conditions , Social constructionism
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406183 , vital:70246
- Description: This study considers the experiences of teachers working with Fetal Alcohol Spectrum Disorders (FASD)-affected children in mainstream schooling. FASD refers to a range of conditions in children resulting from maternal alcohol consumption during pregnancy. Prevalence rates in South Africa are said to be among the highest in the world. There is a paucity of research on FASD-affected children in the Eastern Cape. This study was thus conducted in the Buffalo City Metropolitan Municipality (BCMM). Teachers told stories of their experiences of teaching undiagnosed FASD-affected children in mainstream schooling. The stories reveal the quality of life for children in classrooms and socio-contextual influences of their experiences at school, as well as the responsibilisation of teachers in these settings. This qualitative research is situated within a social constructionism paradigm. The social model of disability was utilised as a theoretical framework for this study to represent the systemic barriers against which FASD-affected learner support in the mainstream school can be conceptualised. A sample of five (5) Black female teachers were interviewed. Data gathering was done by conducting telephonic interviews using the single question inducing narrative, known as SQUIN. A thematic analysis of the data was conducted for the purpose of identifying themes within the data collected. Four superordinate themes emerged from the data, viz. narratives of FASD-affected learners, narratives of parental involvement, narratives of the role of teachers and narratives of stakeholder support. This study reveals a significant level of responsibilisation of teachers. Teachers appear to play a bigger role than just being teachers and they positioned themselves as playing a parental role in relation to FASD-affected learners. FASD-affected learners were positioned as “the problem” in the mainstream classroom whereas the environment is a disabling factor to FASD-affected learners. There is a need for a holistic approach in working with FASD-affected learners from all relevant stakeholders. Key recommendations are made for effective inclusion of FASD-affected learners in mainstream schooling. Future research recommendations include repeating this study in a different geographical area, a focus on senior phase learners and/or a more diverse sample. The findings assist in the development of policies of inclusive education in mainstream schooling and supportive strategies to enhance the developmental trajectory of FASD-affected children. , Thesis (MA) -- Faculty of Humanities, Psychology, 2022
- Full Text:
- Date Issued: 2022-10-14
Issues and challenges facing school libraries: a case study of selected primary schools in Gauteng Province, South Africa
- Paton-Ash, Margaret Sanderson
- Authors: Paton-Ash, Margaret Sanderson
- Date: 2012
- Subjects: School libraries -- South Africa -- Gauteng Libraries and state -- South Africa -- Gauteng Libraries and schools -- South Africa -- Gauteng Library planning -- South Africa -- Gauteng Libraries and education -- South Africa -- Gauteng
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1660 , http://hdl.handle.net/10962/d1003543
- Description: Fewer than 8% of schools in South Africa have functioning libraries. There is no national policy for school libraries which compels School Governing Bodies and principals to have a library in their schools. This qualitative study, based on grounded theory, investigated ten primary schools in Gauteng that had libraries, or were in the process of setting up a library with the intention of providing a rich description of the issues and challenges facing these schools. The schools were chosen on the basis of location (Soweto and Johannesburg), and the school fees that were paid in a continuum from low/no fee paying schools to the fee paying ex Model-C schools. The resourcing of the school library, the operation of the school library and the role of the library were examined. The findings highlight the lack of a national policy, of school library posts, the theft of computers as a result of the lack of security, as well as the lack of understanding by teachers of the role the library in teaching and learning. Furthermore, there appears to be a disjuncture between the trends evident in the international literature on school libraries and what is actually happening in the primary school libraries in my study. Most significantly low/no fee paying schools with libraries were the exception and those that did have libraries were as a result of the enthusiasm and efforts of one or two individuals with the backing of the principal. Ex-Model C schools had the advantage in terms of the provision and staffing of school libraries as they had the funds to support them and, in some cases, a school librarian, provided that the principal supported the idea of a school library. In all the schools, the belief in the importance of the library regarding reading and literacy was the main motivating factor in establishing or maintaining the school library. A growing public awareness of the value of school libraries was an encouraging trend that emerged from my study.
- Full Text:
- Date Issued: 2012
- Authors: Paton-Ash, Margaret Sanderson
- Date: 2012
- Subjects: School libraries -- South Africa -- Gauteng Libraries and state -- South Africa -- Gauteng Libraries and schools -- South Africa -- Gauteng Library planning -- South Africa -- Gauteng Libraries and education -- South Africa -- Gauteng
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1660 , http://hdl.handle.net/10962/d1003543
- Description: Fewer than 8% of schools in South Africa have functioning libraries. There is no national policy for school libraries which compels School Governing Bodies and principals to have a library in their schools. This qualitative study, based on grounded theory, investigated ten primary schools in Gauteng that had libraries, or were in the process of setting up a library with the intention of providing a rich description of the issues and challenges facing these schools. The schools were chosen on the basis of location (Soweto and Johannesburg), and the school fees that were paid in a continuum from low/no fee paying schools to the fee paying ex Model-C schools. The resourcing of the school library, the operation of the school library and the role of the library were examined. The findings highlight the lack of a national policy, of school library posts, the theft of computers as a result of the lack of security, as well as the lack of understanding by teachers of the role the library in teaching and learning. Furthermore, there appears to be a disjuncture between the trends evident in the international literature on school libraries and what is actually happening in the primary school libraries in my study. Most significantly low/no fee paying schools with libraries were the exception and those that did have libraries were as a result of the enthusiasm and efforts of one or two individuals with the backing of the principal. Ex-Model C schools had the advantage in terms of the provision and staffing of school libraries as they had the funds to support them and, in some cases, a school librarian, provided that the principal supported the idea of a school library. In all the schools, the belief in the importance of the library regarding reading and literacy was the main motivating factor in establishing or maintaining the school library. A growing public awareness of the value of school libraries was an encouraging trend that emerged from my study.
- Full Text:
- Date Issued: 2012
A green approach for the synthesis of symmetrical and unsymmetrical 1,2,4,5-tetraoxanes as anti-protozoal agents
- Authors: Cossa, Teresa Manuel
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/192786 , vital:45264
- Description: Thesis (PhD) -- Faculty of Science, Chemistry, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Cossa, Teresa Manuel
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/192786 , vital:45264
- Description: Thesis (PhD) -- Faculty of Science, Chemistry, 2021
- Full Text:
- Date Issued: 2021-10-29