https://commons.ru.ac.za/vital/access/manager/Index en-us 5 https://commons.ru.ac.za/vital/access/manager/Repository/vital:4148 Wed 12 May 2021 22:47:13 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:4154 Wed 12 May 2021 19:07:50 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:29112 Wed 12 May 2021 18:53:14 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:4145 Wed 12 May 2021 16:39:32 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:4172 Wed 12 May 2021 16:06:18 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:3886 20% of its initial activity after 9 consecutive reuse cycles. When exposed to fixed concentrations of carbofuran or demeton-S-methyl sulfone, immobilised acetylcholinesterase showed similar inhibition characteristics to that of the free enzyme. The decrease in enzyme activity observed after immobilisation to the nanofibres may have been due to several reasons which include some enzyme molecules being immobilised in structural conformations which reduced substrate access to the catalytic site, participation of the catalytic residues in immobilisation and enzyme denaturation due to the reaction conditions used for acetylcholinesterase immobilisation. Similar observations have been widely reported in literature and this is one of the major drawbacks of enzyme immobilisation. In conclusion, nylon-6:chitosan electrospun nanofibres were shown to be suitable supports for facile acetylcholinesterase immobilisation and the immobilised enzyme has potential for use in pesticide detection. Future recommendations for this study include a comparative study of the GA cross-linking method for AChE immobilisation which will lead to more intensely bound enzyme molecules to prevent non-specific binding. An investigation into the effect of inhibitors on stored immobilised AChE, as well as reactivation and reuse studies, may also be useful for determining the cost-effectiveness of reusing immobilised AChE for pesticide detection in environmental water samples. Several models have been designed for the determination of the kinetic parameters for immobilised enzymes. These take into account the mass transfer resistance as well as the overall charge of the immobilisation matrix. The use of these models to analyse experimental data will give a clear understanding of the effects of immobilisation on enzyme activity]]> Thu 13 May 2021 08:19:14 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:4153 Thu 13 May 2021 07:14:07 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:29165 Thu 13 May 2021 07:11:47 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:4096 Thu 13 May 2021 05:48:14 SAST ]]>