https://commons.ru.ac.za/vital/access/manager/Index en-us 5 https://commons.ru.ac.za/vital/access/manager/Repository/vital:72613 Wed 22 May 2024 03:13:34 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:72799 Tue 28 May 2024 14:19:00 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:72801 Tue 28 May 2024 14:18:01 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:72802 Tue 28 May 2024 14:17:31 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:72803 10 cells/cm. Each experiment normally includes a blank control, containing medium without the cells.28,45 Non-contaminated HeLa cells (6.57 x 105 cells per well) in media were allowed to grow in the incubator under an atmosphere of 5% CO2 at 37 0C for 24 h. To each well was dispensed 200 µL of HeLa culture, containing 6.57 x 105 cells under LabEAir laminar flow hood (Vivid Air, South Africa); 20 µL of resazurin dye (Sigma TOX-8) and test compound (50 µL) were added, which were then incubated in the presence of 5% CO2 at 37 0C for 24 hours in a shaker, to enhance the distribution of the dye. The absorbance of each well was measured with Bio-tek Power Wave X fluorometer (Beijing, China), and increases in fluorescence was monitored at a wavelength of 590 nm, using an excitation wavelength of 560 nm.]]> Tue 28 May 2024 14:16:45 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:45519 Tue 07 May 2024 12:28:00 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:45516 n-hexane extract (IC50=25.18 μg/mL). It also showed mild cytotoxicity (IC50>100 μg/mL; selectivity index >4) compared to the reference drug emetine (IC50=0.013 μg/mL). The known sterol, ergostan-5,7,22-trien-3-ol, was isolated and characterized from the extract. Conclusion: This study reporting for the first time the antiplasmodial activity of P. ostreatus revealed its nutraceutical potential in the management of malaria.]]> Tue 07 May 2024 12:27:04 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:45331 Tue 07 May 2024 12:25:34 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:45163 Tue 07 May 2024 12:24:14 SAST ]]> https://commons.ru.ac.za/vital/access/manager/Repository/vital:72745 Thu 23 May 2024 15:57:08 SAST ]]>